CN109280611A - A kind of expansion RNA extracts kit - Google Patents
A kind of expansion RNA extracts kit Download PDFInfo
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- CN109280611A CN109280611A CN201811124186.6A CN201811124186A CN109280611A CN 109280611 A CN109280611 A CN 109280611A CN 201811124186 A CN201811124186 A CN 201811124186A CN 109280611 A CN109280611 A CN 109280611A
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- box body
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- connecting plate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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Abstract
The present invention relates to a kind of expansion RNA extracts kits, including box component, the pipe support component being flexibly connected with box component;The box component includes the first successively longitudinally-hinged box body, the second box body, third box body, the 4th box body, is hinged by connecting plate between first box body and second box body, the third box body and the 4th box body.The present invention provides a kind of expansion RNA extracts kits, can optimize preparation of reagents process, and convenient for distinguishing the storage of different reagents, reagent bottle and test tube in entire extraction process are placed at one, ensure that the accuracy of test data, improve work efficiency.
Description
Technical field
The present invention relates to experiment reagent box fields, and in particular to a kind of expansion RNA extracts kit.
Background technique
RNA is using a chain of DNA as template, and with base pair complementarity principle, one for transcribing and being formed is single-stranded, mainly
Function is the expression for realizing hereditary information on protein, is the bridge in hereditary information transmittance process.Kit is for various
The RNA extraction of plant, animal, microorganism, has portion to illustrate operation instructions in each kit.Experimenter only needs to press
Step operation on book as directed.It is very convenient without preparing other reagents when use, it is suitable for the rapidly extracting of RNA.
Extracted RNA integrality is good, with high purity, tests after can be used for molecular biology.Kit is also for holding detection chemistry
The box of the chemical reagent such as ingredient, medicament residue, viral species.The generation of kit makes experimenter get rid of heavy reagent
Process for preparation optimizes test program.
Currently, in order to meet the needs of test and accurate test data, relevant speciality personage is to being applicable in respective field
Kit done further improvement.But existing RNA extracts kit could not preferably optimize preparation of reagents process,
Completion cannot be concentrated to test, and the storage for the different reagents that are not easily distinguishable, affect the accuracy of test data.
Summary of the invention
The present invention provides a kind of expansion RNA extracts kits, can optimize preparation of reagents process, convenient for distinguishing different examinations
The storage of agent, reagent bottle and test tube in entire extraction process are placed at one, ensure that the accuracy of test data, improve
Working efficiency.
The technical solution adopted by the invention is as follows:
A kind of expansion RNA extracts kit, including box component, the pipe support component being flexibly connected with box component;Institute
Stating box component includes the first successively longitudinally-hinged box body, the second box body, third box body, the 4th box body, first box body
It is hinged by connecting plate between second box body, the third box body and the 4th box body.
The working principle and process of the technical program are as follows:
To extract first reagent bottle needed for RNA be put into the first box body, the second box body, third box body, in the 4th box body,
Pipe support component is placed in the top of box component, removes pipe support component in use process, group is installed on experimental bench;Successively
The first box body, the second box body, third box body, the 4th box body being hinged are unfolded by the good connecting plate in hinged place, are rotated freely
To the state of appropriate experimental;Centrifuge tube is placed on pipe support component, starts to test according to extraction step.
Further, the first box body, the second box body, third box body, the 4th box body are the square column type of same size.
Further, the width of connecting plate and the first box body, the second box body, third box body or the width of the 4th box body side
Unanimously.The equivalent width of first box body of square column type, the second box body, third box body, the 4th box body and connecting plate, so that box body
For component under the different conditions of connecting plate unfolded and folded, the first box body, the second box body, third box body, the 4th box body can be with
In one line or two rows two column are presented and wait various structures, the structure shape for selecting appropriate experimental to carry out convenient for experimenter side by side
State.
Further, the first box body, the second box body, third box body, the 4th tray interior are longitudinally set there are two reagent bottle
Frame.Reagent bottle frame design so that the first box body, the second box body, third box body, the 4th box body side not by box component
Shell capping, convenient for the reagent bottle in second layer reagent bottle frame of taking.
Further, the first box body longitudinal direction incline of the incline of connecting plate and the first box body and the second box body contact surface is cut with scissors
It connects, another incline and the first box body and the second box body contact surface of connecting plate are located at the first box body longitudinal direction incline cornerwise second
Box body longitudinal direction incline is hinged.
Further, the third box body longitudinal direction incline of the incline of connecting plate and third box body and the 4th box body contact surface is cut with scissors
It connects, another incline and third box body and the 4th box body contact surface of connecting plate are located at third box body longitudinal direction incline the cornerwise 4th
Box body longitudinal direction incline is hinged.When the reagent type needed for testing is more, keeps the second box body and third box body motionless, pass through connection
Plate opens the first box body to, first box body, second box body, third box parallel with third box body with the second box body with the second box body
Body, the 4th box body are in one line, and experimenter can test in the same side, and reagent bottle is placed in box component, in experiment
Dropper etc. can be placed in the reagent frame of first layer, convenient for taking.When the reagent type needed for testing is less, by the first box body
It is rotated by connecting plate to the first box body and the 4th back-to-back state of box body with the 4th box body, at this time the first box body and the 4th box
Body and the second box body and third box body are in mirror settings, and it is real can to carry out multiple groups in order to two experimenters for box component at this time
It tests, each experimenter uses the box component of the same side, improves working efficiency, improves the utilization rate of kit.
Further, the pipe support component includes centrifuge tube shelf, the strut being flexibly connected with centrifuge tube shelf and the strut
The bottom frame of flexible connection;The centrifuge tube shelf bottom four corners are equipped with the groove being adapted to strut, and the bottom frame is close to centrifuge tube shelf
Side be equipped with the groove that is adapted to strut.Strut one end is connected to the groove on the inside of centrifuge tube shelf, other end connection and bottom frame
The groove of inside.
Further, the centrifuge tube shelf is equipped with centrifugation pore and labeled slots.
The technical program creatively by the first box body and the second box body, is provided with phase between third box body and the 4th box body
Mutually articulated connection plate facilitates experimenter to operate the case where box component is expanded to convenient for different experiments demand;Box when expansion
Reagent bottle frame in body component is external, takes convenient for experimenter, and reagent frame when closure prevents from being not used all towards inside
Kit by dust pollution, influence to test accuracy.Centrifuge tube shelf is flexibly connected with bottom frame by strut, and kit is saved
Space, and centrifuge tube is needed in refrigerated environment in some experimental procedures for extracting RNA, can will be placed in bottom frame at this time a small amount of
Ice cube does not need to increase additional means.Kit of the invention, which is more laid out, more to be rationalized, and is easy to use, and work is improved
Make efficiency.Centrifuge tube is placed in outside by centrifuge tube shelf, observes in real time convenient for laboratory technician, improves conventional efficient.
In conclusion compared with the prior art, the invention has the advantages that:
(1) kit is cut with scissors the first box body and the second box body, third box body and the 4th box body by connecting plate in the present invention
It connects, so that the first box body and the 4th box body rotation direction are larger in use process, in one line or two rows two column can be presented
Equal various structures, provide kinds of experiments structure and usage mode;The utilization rate of kit is improved, is taken reagent convenient for experimenter
Bottle, reagent bottle concentrate at one, improve conventional efficient, lower Human Error Rate;
(2) in the present invention box component and pipe support component design, preparation of reagents process can be optimized, convenient for the different examinations of differentiation
The storage of agent, reagent bottle and test tube in entire extraction process are placed at one, ensure that the accuracy of test data, improve
Working efficiency avoids influencing each other in use process, improves the utilization rate of box body, and optimization layout improves service efficiency;
(3) so that the reagent frame under unused state is arranged inwardly, which prevents ash for the design of connecting plate of the present invention
Dirt is fallen into kit, pollutes reagent;
(4) the configuration of the present invention is simple, it is easy to operate, preparation of reagents process can be optimized, convenient for distinguishing the storage of different reagents,
The accuracy that ensure that test data, improves work efficiency.
Detailed description of the invention
Fig. 1 be in the present invention box component side by side when structural schematic diagram
Fig. 2 is structure structure chart when box component is unfolded in the present invention
Fig. 3 is a kind of structural schematic diagram of expansion RNA extracts kit in the present invention
Fig. 4 is structural schematic diagram when pipe support component uses in the present invention
Fig. 5 is the structural schematic diagram of bottom frame and strut in pipe support component in the present invention
In the figure, it is marked as the first box body of 1-, the second box body of 2-, 3- third box body, the 4th box body of 4-, 5- connecting plate, 6- examination
Agent bottle frame, 7- centrifuge tube shelf, 8- strut, 9- bottom frame, 10- groove, 11- are centrifuged pore, 12- labeled slots.
Specific embodiment
All features disclosed in this specification can be with any other than mutually exclusive feature and/or step
Mode combines.
In order to make those skilled in the art more fully understand technical solution of the present invention, below with reference to Fig. 1-3 and specifically
Embodiment the present invention is described in further detail.
Embodiment 1
A kind of expansion RNA extracts kit, including box component, the pipe support component being flexibly connected with box component;Institute
Stating box component includes the first successively longitudinally-hinged box body 1, the second box body 2, third box body 3, the 4th box body 4, and described first
It is hinged by connecting plate 5 between box body 1 and second box body 2, the third box body 3 and the 4th box body 4.It is first
To first extract reagent bottle needed for RNA be put into the first box body 1, the second box body 2, third box body 3, in the 4th box body 4, pipe support group
Part is placed in the top of box component, removes pipe support component in use process, group is installed on experimental bench;Successively mutually cut with scissors
The first box body 1, the second box body 2, the third box body 3, the 4th box body 4 connect is unfolded by the good connecting plate 5 in hinged place, is rotated freely
To the state of appropriate experimental;Centrifuge tube is placed on pipe support component, starts to test according to extraction step.
Embodiment 2
Based on embodiment 1, the first box body 1, the second box body 2, third box body 3, the 4th box body 4 are the pros of same size
Column type.
Embodiment 3
Based on embodiment 2, the width of connecting plate 5 and the first box body 1, the second box body 2,4 side of third box body 3 or the 4th box body
The equivalent width in face.First box body 1 of square column type, the second box body 2, third box body 3, the 4th box body 4 and connecting plate 5 width
Unanimously, so that box component is under the different conditions of connecting plate 5 unfolded and folded, the first box body 1, the second box body 2, third box
The in one line or two rows two column various structures such as side by side can be presented in body 3, the 4th box body 4, be suitable for convenient for experimenter's selection
Test the configuration state carried out.
Embodiment 4
Based on embodiment 1, the first box body 1, the second box body 2, third box body 3, inside the 4th box body 4 it is longitudinal set there are two
Reagent bottle frame 6.Reagent bottle frame 6 design so that the first box body 1, the second box body 2, third box body 3, the 4th box body 4 side not
It is covered by the shell of box component, convenient for the reagent bottle in second layer reagent bottle frame 6 of taking.
Embodiment 5
Based on embodiment 3, the first box body 1 of 2 contact surface of the incline of connecting plate 5 and the first box body 1 and the second box body is longitudinal
Incline is hinged, and another incline of connecting plate 5 and the first box body 1 and 2 contact surface of the second box body are located at the longitudinal incline pair of the first box body 1
The longitudinal incline of the second box body 2 of linea angulata is hinged.
Embodiment 6
Based on embodiment 3, the incline of connecting plate 5 and the third box body 3 of 4 contact surface of third box body 3 and the 4th box body are longitudinal
Incline is hinged, and another incline and third box body 3 and 4 contact surface of the 4th box body of connecting plate 5 are located at the longitudinal incline pair of third box body 3
The longitudinal incline of the 4th box body 4 of linea angulata is hinged.When the reagent type needed for testing is more, the second box body 2 and third box body 3 are kept
It is motionless, first box body 1 is opened with the second box body 2 to, first box parallel with third box body 3 with the second box body 2 by connecting plate 5
Body 1, the second box body 2, third box body 3, the 4th box body 4 are in one line, and experimenter can test in the same side, and reagent bottle is equal
It is placed in box component, dropper in experiment etc. can be placed in the reagent frame of first layer, convenient for taking.The reagent needed for testing
It is when type is less, the first box body 1 and the 4th box body 4 is back-to-back by the rotation of connecting plate 5 to the first box body 1 and the 4th box body 4
State, the first box body 1 and the 4th box body 4 and the second box body 2 and third box body 3 are in mirror settings at this time, at this time box component
Multiple groups experiment can be carried out in order to two experimenters, each experimenter uses the box component of the same side, improves work effect
Rate improves the utilization rate of kit.
Embodiment 7
Based on embodiment 1, the pipe support component includes centrifuge tube shelf 7, the strut being flexibly connected with centrifuge tube shelf 78 and institute
State the bottom frame 9 of the flexible connection of strut 8;7 bottom four corners of centrifuge tube shelf are equipped with the groove 10 being adapted to strut 8, the bottom frame 9
The groove 10 being adapted to strut 8 is equipped with close to the side of centrifuge tube shelf 7.8 one end of strut is connected to the groove of 7 inside of centrifuge tube shelf
10, the groove 10 of other end connection and 9 inside of bottom frame.
Embodiment 8
Based on embodiment 7, the centrifuge tube shelf 7 is equipped with centrifugation pore 11 and labeled slots 12.
The specific embodiment of the application above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
The limitation to the application protection scope therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, under the premise of not departing from technical scheme design, various modifications and improvements can be made, these belong to this
The protection scope of application.
Claims (8)
1. a kind of expansion RNA extracts kit, it is characterised in that: including box component, the pipe being flexibly connected with box component
Frame component;The box component includes successively vertical hinged the first box body (1), the second box body (2), third box body (3), the 4th
Box body (4), between first box body (1) and second box body (2), the third box body (3) and the 4th box body (4)
It is hinged by connecting plate (5).
2. a kind of expansion RNA extracts kit according to claim 1, it is characterised in that: the first box body (1), second
Box body (2), third box body (3), the 4th box body (4) are the square column type of same size.
3. a kind of expansion RNA extracts kit according to claim 2, it is characterised in that: the width of connecting plate (5) with
First box body (1), the second box body (2), third box body (3) or the equivalent width of the 4th box body (4) side.
4. a kind of expansion RNA extracts kit according to claim 1, it is characterised in that: the first box body (1), second
The internal overlapping of box body (2), third box body (3), the 4th box body (4) is equipped with two reagent bottle frames (6).
5. a kind of expansion RNA extracts kit according to claim 3, it is characterised in that: the vertical side of connecting plate (5)
Rib and the vertical incline of first box body (1) of the first box body (1) and the second box body (2) contact surface are hinged, and the another of connecting plate (5) erects
Second be located on the first box body (1) vertical incline diagonal line on incline and the first box body (1) and the second box body (2) contact surface
The vertical incline of box body (2) is hinged.
6. a kind of expansion RNA extracts kit according to claim 3, it is characterised in that: the vertical side of connecting plate (5)
Rib and the vertical incline of the third box body (3) of third box body (3) and the 4th box body (4) contact surface are hinged, and the another of connecting plate (5) erects
The 4th be located on third box body (3) vertical incline diagonal line on incline and third box body (3) and the 4th box body (4) contact surface
The vertical incline of box body (4) is hinged.
7. a kind of expansion RNA extracts kit according to claim 1, it is characterised in that: the pipe support component includes
Centrifuge tube shelf (7), the strut (8) being flexibly connected with centrifuge tube shelf (7), the bottom frame (9) being flexibly connected with the strut (8);Institute
It states centrifuge tube shelf (7) bottom four corners and is equipped with the groove (10) being adapted to strut (8), the bottom frame (9) is close to centrifuge tube shelf (7)
Side is equipped with the groove (10) being adapted to strut (8).
8. a kind of expansion RNA extracts kit according to claim 7, it is characterised in that: on the centrifuge tube shelf (7)
Equipped with centrifugation pore (11) and labeled slots (12).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811124186.6A CN109280611A (en) | 2018-09-26 | 2018-09-26 | A kind of expansion RNA extracts kit |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811124186.6A CN109280611A (en) | 2018-09-26 | 2018-09-26 | A kind of expansion RNA extracts kit |
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CN109280611A true CN109280611A (en) | 2019-01-29 |
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CN201811124186.6A Withdrawn CN109280611A (en) | 2018-09-26 | 2018-09-26 | A kind of expansion RNA extracts kit |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110157702A (en) * | 2019-05-24 | 2019-08-23 | 中国水产科学研究院南海水产研究所深圳试验基地 | It is a kind of for extracting the acquisition station of coral RNA sample |
-
2018
- 2018-09-26 CN CN201811124186.6A patent/CN109280611A/en not_active Withdrawn
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110157702A (en) * | 2019-05-24 | 2019-08-23 | 中国水产科学研究院南海水产研究所深圳试验基地 | It is a kind of for extracting the acquisition station of coral RNA sample |
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Application publication date: 20190129 |
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