CN104988052A - Quick virus detection kit and using method thereof - Google Patents
Quick virus detection kit and using method thereof Download PDFInfo
- Publication number
- CN104988052A CN104988052A CN201510356978.6A CN201510356978A CN104988052A CN 104988052 A CN104988052 A CN 104988052A CN 201510356978 A CN201510356978 A CN 201510356978A CN 104988052 A CN104988052 A CN 104988052A
- Authority
- CN
- China
- Prior art keywords
- medicament
- detection kit
- virus detection
- kit
- sample detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/18—Togaviridae; Flaviviridae
- G01N2333/183—Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
Abstract
The invention relates to a quick virus detection kit and a using method thereof. The quick virus detection kit comprises a kit cover 1 and kit body 2, wherein a reagent storage and delivery device, a reagent mixing device, a quantitative suction device and a sample detection device are arranged in the kit body 2, the reagent storage and delivery device delivers reagents for the reagent mixing device, and the quantitative suction device is used for quantitatively sucking mixed reagents from the reagent mixing device to the sample detection device. The quick virus detection kit adopts a reagent packet to conduct encapsulation and metering on detection reagents, adopts the reagent storage and delivery device to perform delivery, enables reagent storage time to be longer, enables metering to be more accurate, enables the reagents not to be easily polluted and also enables an inspection result to be more accurate. In addition, the reagents can be repeatedly used for multiple times, and inspection efficiency is improved.
Description
Technical field
The present invention relates to a kind of medical detection kit, be specifically related to a kind of speed and survey virus detection kit and using method thereof.
Background technology
In recent years, African swine fever has been spanned to the ground such as Armenia, Ukraine, Russia in the Eurasia by the African continent, cause great threat to China.African swine fever once import into by raise pigs industrial belt come destructive strike.
Pestivirus suis copies in tenuigenin, can not aggegation red blood corpuscle, has related antigen with bovine diarrhea virus.This virus is responsive to ether, stronger to resistibilitys such as temperature, ultraviolet, chemostefilants.Utilizing RT-PCR to detect virus is a kind of very sensitive method.Detection ordinary method due to PCR product adopts agarose gel electrophoresis to carry out, observe under ultraviolet light after needing to carry out ethidium bromide staining after electrophoresis, but ethidium bromide has moderate toxicity and carinogenicity to people, easily causes environmental pollution, this also seriously limits the popularization of this method.
Summary of the invention
For solving the above-mentioned technical problem existed in prior art, the object of this invention is to provide a kind of speed and surveying virus detection kit and using method thereof.
Technical scheme of the present invention is as follows
A kind of speed surveys virus detection kit, comprise lid 1 and box body 2, medicament storage e Foerderanlage, medicament mixing device, quantitatively suction unit and sample detection means is provided with in described box body 2, medicament delivery device is medicament mixing device delivery of medicaments, and quantitative suction unit is used for quantitatively being drawn in sample detection means by the medicament mixed from medicament mixing device.
Described medicament storage e Foerderanlage comprises the unreeling wheel 4 be positioned on the left of box body 2, is wrapped in the medicament band 3 on unreeling wheel 4, is positioned at the winding wheel 7 on the upside of box body 2, is positioned at the squeegee roller 6 below winding wheel 7, is positioned at the seal opening device 5 of squeegee roller front end.
Described seal opening device is blade, scissors or sharp keen pin.
Described medicament storage device is provided with 1 ~ 5 cover.
Described medicament storage device is provided with 2 covers, and the medicament stored in a set of medicament band is antiviral antibody, and the medicament stored in another set of medicament band is enzyme plate.
Described medicament band is made up of the kit 31 and sealing 32 that medicament is housed.
The electromagnetic shaker 11 that described medicament mixing device comprises mixing tank 10 and is positioned at below mixing tank 10.
Described quantitative suction unit comprises pipeline 9 in from medicament mixing device to sample detection means and for providing the volume pump 8 of suction power.
Described sample detection means is sample detection bottle 12.
Described speed is surveyed virus detection kit and is also comprised the computer chip controlling winding wheel rolling, squeegee roller, seal opening device and volume pump and aspirate.
The material that described sample detection bottle is is polypropylene or glass material.
Preferably, it is that speed surveys Pestivirus suis detection kit that described speed surveys virus detection kit.
A kind of above-mentioned speed surveys the using method of virus detection kit, it is characterized in that, comprise the following steps, first be arranged on unreeling wheel 4 by medicament band 3, get testing sample and add in sample detection bottle 12, winding wheel 7 carries out in the process of rolling, medicament band is punctured by seal opening device 5, and squeezed pinch roller 6 extrudes, medicament enters in mixing tube 10 and mixes under the vibrations of electromagnetic shaker 11, the detection being drawn into complete paired samples in sample detection bottle 12 that then measured pump 8 is quantitative.
Compared to the prior art beneficial effect of the present invention is:
Speed of the present invention is surveyed virus detection kit and is adopted kit encapsulate detection reagent and measure, and adopt medicament storage e Foerderanlage to carry, make the medicament storage time longer, it is more accurate to measure, be not easy to pollute, also make assay more accurate, simultaneously can repeatedly Reusability by test kit, improve the efficiency of inspection.
Accompanying drawing explanation
Fig. 1 is the structural representation of the speed survey virus detection kit in embodiment 1;
Fig. 2 is the structural representation of medicament band;
Wherein: 1 is lid, 2 is box body, and 3 is medicament band, and 31 is kit, and 32 is sealing, and 4 is unreeling wheel, and 5 is seal opening device, and 6 is squeegee roller, and 7 is winding wheel, and 8 is volume pump, and 9 is pipeline, and 10 is mixing tank, and 11 is electromagnetic shaker, and 12 is sample detection bottle.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
A kind of speed surveys virus detection kit as shown in Figure 1, 2, comprise lid 1 and box body 2, medicament storage e Foerderanlage, medicament mixing device, quantitatively suction unit and sample detection means is provided with in described box body 2, medicament delivery device is medicament mixing device delivery of medicaments, and quantitative suction unit is used for quantitatively being drawn in sample detection means by the medicament mixed from medicament mixing device.
Described medicament storage e Foerderanlage comprises the unreeling wheel 4 be positioned on the left of box body 2, is wrapped in the medicament band 3 on unreeling wheel 4, is positioned at the winding wheel 7 on the upside of box body 2, is positioned at the squeegee roller 6 below winding wheel 7, is positioned at the seal opening device 5 of squeegee roller front end.
Described seal opening device is blade, and described medicament storage device is provided with 2 covers, and the medicament stored in a set of medicament band is antiviral antibody, and the medicament stored in another set of medicament band is enzyme plate.Described medicament band is made up of the kit 31 and sealing 32 that medicament is housed.The electromagnetic shaker 11 that described medicament mixing device comprises mixing tank 10 and is positioned at below mixing tank 10.Described quantitative suction unit comprises pipeline 9 in from medicament mixing device to sample detection means and for providing the volume pump 8 of suction power.Described sample detection means is sample detection bottle 12.Described speed is surveyed virus detection kit and is also comprised the computer chip controlling winding wheel rolling, squeegee roller, seal opening device and volume pump and aspirate.The material that described sample detection bottle is is polypropylene material.
A kind of above-mentioned speed surveys the using method of virus detection kit, it is characterized in that, comprise the following steps, first be arranged on unreeling wheel 4 by medicament band 3, get testing sample and add in sample detection bottle 12, winding wheel 7 carries out in the process of rolling, medicament band is punctured by seal opening device 5, and squeezed pinch roller 6 extrudes, medicament enters in mixing tube 10 and mixes under the vibrations of electromagnetic shaker 11, the detection being drawn into complete paired samples in sample detection bottle 12 that then measured pump 8 is quantitative.
Embodiment 2
A kind of speed surveys virus detection kit, comprise lid 1 and box body 2, medicament storage e Foerderanlage, medicament mixing device, quantitatively suction unit and sample detection means is provided with in described box body 2, medicament delivery device is medicament mixing device delivery of medicaments, and quantitative suction unit is used for quantitatively being drawn in sample detection means by the medicament mixed from medicament mixing device.
Described medicament storage e Foerderanlage comprises the unreeling wheel 4 be positioned on the left of box body 2, is wrapped in the medicament band 3 on unreeling wheel 4, is positioned at the winding wheel 7 on the upside of box body 2, is positioned at the squeegee roller 6 below winding wheel 7, is positioned at the seal opening device 5 of squeegee roller front end.
Described seal opening device is scissors, and described medicament storage device is provided with 2 covers, and the medicament stored in a set of medicament band is antiviral antibody, and the medicament stored in another set of medicament band is enzyme plate.Described medicament band is made up of the kit 31 and sealing 32 that medicament is housed.The electromagnetic shaker 11 that described medicament mixing device comprises mixing tank 10 and is positioned at below mixing tank 10.Described quantitative suction unit comprises pipeline 9 in from medicament mixing device to sample detection means and for providing the volume pump 8 of suction power.Described sample detection means is sample detection bottle 12.Described speed is surveyed virus detection kit and is also comprised the computer chip controlling winding wheel rolling, squeegee roller, seal opening device and volume pump and aspirate.The material that described sample detection bottle is is glass material.
A kind of above-mentioned speed surveys the using method of virus detection kit, it is characterized in that, comprise the following steps, first be arranged on unreeling wheel 4 by medicament band 3, get testing sample and add in sample detection bottle 12, winding wheel 7 carries out in the process of rolling, medicament band is punctured by seal opening device 5, and squeezed pinch roller 6 extrudes, medicament enters in mixing tube 10 and mixes under the vibrations of electromagnetic shaker 11, the detection being drawn into complete paired samples in sample detection bottle 12 that then measured pump 8 is quantitative.
Embodiment 3
A kind of speed surveys virus detection kit, comprise lid 1 and box body 2, medicament storage e Foerderanlage, medicament mixing device, quantitatively suction unit and sample detection means is provided with in described box body 2, medicament delivery device is medicament mixing device delivery of medicaments, and quantitative suction unit is used for quantitatively being drawn in sample detection means by the medicament mixed from medicament mixing device.
Described medicament storage e Foerderanlage comprises the unreeling wheel 4 be positioned on the left of box body 2, is wrapped in the medicament band 3 on unreeling wheel 4, is positioned at the winding wheel 7 on the upside of box body 2, is positioned at the squeegee roller 6 below winding wheel 7, is positioned at the seal opening device 5 of squeegee roller front end.
Described seal opening device is sharp keen pin, and described medicament storage device is provided with 4 covers, and the medicament stored in two cover medicament bands is antiviral antibody, and the medicament stored in other two cover medicament bands is enzyme plate.Described medicament band is made up of the kit 31 and sealing 32 that medicament is housed.The electromagnetic shaker 11 that described medicament mixing device comprises mixing tank 10 and is positioned at below mixing tank 10.Described quantitative suction unit comprises pipeline 9 in from medicament mixing device to sample detection means and for providing the volume pump 8 of suction power.Described sample detection means is sample detection bottle 12.Described speed is surveyed virus detection kit and is also comprised the computer chip controlling winding wheel rolling, squeegee roller, seal opening device and volume pump and aspirate.The material that described sample detection bottle is is polypropylene material.
A kind of above-mentioned speed surveys the using method of virus detection kit, it is characterized in that, comprise the following steps, first be arranged on unreeling wheel 4 by medicament band 3, get testing sample and add in sample detection bottle 12, winding wheel 7 carries out in the process of rolling, medicament band is punctured by seal opening device 5, and squeezed pinch roller 6 extrudes, medicament enters in mixing tube 10 and mixes under the vibrations of electromagnetic shaker 11, the detection being drawn into complete paired samples in sample detection bottle 12 that then measured pump 8 is quantitative.
Above-described embodiment is only the preferred embodiment of the present invention, and should not be construed as limitation of the invention, and those skilled in the art, according to announcement of the present invention, do not depart from improvement that scope makes and amendment all should within protection scope of the present invention.
Claims (10)
1. a speed surveys virus detection kit, it is characterized in that, described speed is surveyed virus detection kit and is comprised lid (1) and box body (2), medicament storage e Foerderanlage, medicament mixing device, quantitatively suction unit and sample detection means is provided with in described box body (2), medicament delivery device is medicament mixing device delivery of medicaments, and quantitative suction unit is used for quantitatively being drawn in sample detection means by the medicament mixed from medicament mixing device.
2. a kind of speed according to claim 1 surveys virus detection kit, it is characterized in that, described medicament storage e Foerderanlage comprises the unreeling wheel (4) being positioned at box body (2) left side, be wrapped in the medicament band (3) on unreeling wheel (4), be positioned at the winding wheel (7) of box body (2) upside, be positioned at the squeegee roller (6) of winding wheel (7) below, be positioned at the seal opening device (5) of squeegee roller front end.
3. a kind of speed according to claim 2 surveys virus detection kit, it is characterized in that, described medicament storage device is provided with 1 ~ 5 cover.
4. a kind of speed according to claim 3 surveys virus detection kit, and it is characterized in that, described medicament storage device is provided with 2 covers, and the medicament stored in a set of medicament band is antiviral antibody, and the medicament stored in another set of medicament band is enzyme plate.
5. a kind of speed according to claim 2 surveys virus detection kit, it is characterized in that, described medicament band is made up of the kit (31) and sealing (32) that medicament is housed.
6. a kind of speed according to claim 1 surveys virus detection kit, it is characterized in that, described medicament mixing device comprises mixing tank (10) and is positioned at the electromagnetic shaker (11) of mixing tank (10) below.
7. a kind of speed according to claim 1 surveys virus detection kit, it is characterized in that, described quantitative suction unit comprises pipeline (9) in from medicament mixing device to sample detection means and for providing the volume pump (8) of suction power.
8. a kind of speed according to claim 1 surveys virus detection kit, and it is characterized in that, described sample detection means is sample detection bottle (12).
9. a kind of speed according to claim 8 surveys virus detection kit, and it is characterized in that, the material that described sample detection bottle is is polypropylene or glass material.
10. survey the using method of virus detection kit as the speed as described in arbitrary in claim 1 ~ 9 for one kind, it is characterized in that, comprise the following steps, first medicament band (3) is arranged on unreeling wheel (4), getting testing sample adds in sample detection bottle (12), winding wheel (7) carries out in the process of rolling, medicament band is punctured by seal opening device (5), and squeezed pinch roller (6) extruding, medicament enters in mixing tube (10) and mixes under the vibrations of electromagnetic shaker (11), then the detection being drawn into complete paired samples in sample detection bottle (12) that measured pump (8) is quantitative.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510356978.6A CN104988052A (en) | 2015-06-25 | 2015-06-25 | Quick virus detection kit and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510356978.6A CN104988052A (en) | 2015-06-25 | 2015-06-25 | Quick virus detection kit and using method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN104988052A true CN104988052A (en) | 2015-10-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510356978.6A Pending CN104988052A (en) | 2015-06-25 | 2015-06-25 | Quick virus detection kit and using method thereof |
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| CN (1) | CN104988052A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202699599U (en) * | 2012-08-23 | 2013-01-30 | 成都恒瑞制药有限公司 | Medicine box outputting medicine in limited volume |
| CN202830011U (en) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | Automated nucleic acid extraction platform |
| CN103252006A (en) * | 2007-07-06 | 2013-08-21 | 维克多瑞传送设备有限公司 | Inhaler |
| CN103278627A (en) * | 2013-05-22 | 2013-09-04 | 扬州大学 | Multi-antigen ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting African swine fever virus antibody |
-
2015
- 2015-06-25 CN CN201510356978.6A patent/CN104988052A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103252006A (en) * | 2007-07-06 | 2013-08-21 | 维克多瑞传送设备有限公司 | Inhaler |
| CN202699599U (en) * | 2012-08-23 | 2013-01-30 | 成都恒瑞制药有限公司 | Medicine box outputting medicine in limited volume |
| CN202830011U (en) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | Automated nucleic acid extraction platform |
| CN103278627A (en) * | 2013-05-22 | 2013-09-04 | 扬州大学 | Multi-antigen ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting African swine fever virus antibody |
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Application publication date: 20151021 |
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