CN203178282U

CN203178282U - ELIsA detection kit for crystal violet

Info

Publication number: CN203178282U
Application number: CN2013200261962U
Authority: CN
Inventors: 不公告发明人
Original assignee: ZHUOZHOU CITY HENGTONGDA SCIENCE AND TRADE CO Ltd
Current assignee: Hebei Elisa Biotech Co ltd
Priority date: 2013-01-18
Filing date: 2013-01-18
Publication date: 2013-09-04
Anticipated expiration: 2023-01-18

Abstract

The utility model relates to an ELIsA (enzyme linked immunosorbent assay) detection kit for quickly detecting crystal violet in aquatic food. The ELIsA detection kit comprises a kit body and a 96-hole microlon ELISA plate arranged in the kit as well as 10 bottles of reagents, and downward concave bottle positions for placing the reagents; and the ELIsA detection kit is characterized in that the microlon ELISA plate is a detection plate which adopts a 96-hole reagent plate as a solid-phase carrier, and antibodies of anti-mouse crystal violet are pre-coated on microporous strips of the kit; the 10 bottles of reagents comprise 6 bottles of standard substance solution, 1 bottle of crystal violet enzyme markers, 1 bottle of color development solution, 1 bottle of stop solution and 1 bottle of sample diluent respectively; and all reagent bottles are provided with corresponding downward concave bottle positions. Compared with the instrument analysis technology, the ELIsA detection kit has the characteristics of convenience in use, high sensitivity and the like, and can play an important role in the detection of crystal violet in aquatic food.

Description

The crystal violet enzyme-linked immunologic detecting kit

Technical field

The present invention relates to the immunological test field, specifically, relate to a kind of enzyme-linked immunologic detecting kit and detection method thereof that detects the crystal violet in the aquatic food.

Background technology

Crystal violet (gentian violet) belongs to triphenylmethane dye, possessing stronger toxic and side effect, is that country forbids using as disinfecting drug, because this dyestuff has special efficacy to the control of aquatic animal fungal infection, and the price cheapness has been bought easily, is therefore often abused in aquaculture.Studies show that the very fast crystal violet that transforms into recessiveness in the body of fish of most crystal violet is present in the muscle, rate of discharge is very low in vivo residual mostly.Therefore, countries in the world are as the skill barrier, and the input of interception China aquatic products is limited to 0.5ng/g under requiring to detect.Domestic media is also paid close attention to this very much.

The employed existing method of inspection of most of enterprise, commonly used have a liquid phase chromatography, exist operation more loaded down with trivial details, consuming time long, need with after derive, disadvantages such as shakiness appear reclaiming in misoperation, cause enterprise's outlet to be rebuffed.

The fast detecting crystal violet quick detection kit of my company can be measured crystal violet in the sample fast and accurately, is that enterprise provides the method to a large amount of sample rapid screening with cheap relatively cost and higher sensitivity and accuracy.

Summary of the invention

Problem to be solved in the utility model provides a kind of crystal violet detection kit, it is easy and simple to handle, detect quick, accurate, highly sensitive, cost is low, good stability, the technology content that needs is not high, can carry out the mensuration of crystal violet residual quantity in a plurality of samples of one-time continuous, has reduced the needed time of detection sample.

In order to achieve the above object, the utility model is by the following technical solutions:

The technical solution of the utility model is:

A kind of enzyme linked immunological kit that detects crystal violet residual quantity in the aquatic food, the recessed bottle position that comprises ELISA Plate, 10 bottles of reagent in 96 holes in box body and the box and put reagent, it is characterized in that: ELISA Plate is to adopt 96 hole agent plate as solid phase carrier, on the kit capillary strip, wrap by the detection of antibodies plate of crystal violet in advance, described 10 bottles of reagent are respectively 6 bottles of standard solutions, 1 bottle of crystal violet enzyme labeling thing, 1 bottle of colour developing liquid, 1 bottle of stop buffer, 1 bottle of sample diluting liquid, and described reagent bottle all is provided with corresponding recessed bottle position.

Further, described box body is carton box, the ELISA Plate in described 96 holes is the polystyrene ELISA Plate, be positioned in the vacuum aluminium foil bag, described standard solution is all used the Brown Glass Brown glass bottles and jars only of white cap, the enzyme labeling thing white translucent PE plastic bottle of the white cap of blue markings, colour developing liquid is with brown PE plastic bottle, stop buffer is made by plastic foam with the translucent PE plastic bottle of white cap, described recessed bottle position with the translucent white PE plastic bottle of the white cap of yellow mark, sample diluting liquid.

Further, described ELISA Plate is made up of outer frame support and removable 12 enzymes mark reaction capillary strips of being placed on it, each dismantled and assembled enzyme mark reaction capillary strip has 8 reacting holes, each reacting hole wraps in advance by the monoclonal antibody of mouse-anti crystal violet, the capacity of described standard solution reagent bottle is the 2ml/ bottle, and the capacity of enzyme labeling thing reagent bottle is 8ml, and the capacity of colour developing liquid reagent bottle is 12ml, the capacity of stop buffer reagent bottle is 12ml, and the capacity of sample diluting liquid reagent bottle is 60ml.

The beneficial effects of the utility model are the residual quantity that can detect crystal violet in the sample fast and effectively, and easy to operation.

Description of drawings

Figure l is the lateral longitudinal sectional view (the long 8.55cm of being) of the utility model elisa plate;

Fig. 2 is the side drawing in side sectional elevation (length is 12. 8cm) of the utility model elisa plate;

Fig. 3 is the vertical view of the utility model elisa plate;

Fig. 4 is the utility model reagent bottle longitudinal profile planimetric map;

Fig. 5 is the vertical view of the utility model fixed foam mould;

Fig. 6 is the side view of the utility model box body and fixed foam mould.

Embodiment:

Referring to accompanying drawing: the pre-bag of enzyme mark reaction capillary strip (2) is fixed on the outer frame support (1) of ELISA Plate by the monoclonal antibody of mouse-anti crystal violet (3), and enzyme mark reaction capillary strip (2) can be with requiring dismounting; The translucent plastic reagent bottle (4) of white cap is used for encapsulation 60ml sample diluting liquid; The translucent plastic reagent bottle (5) of blue markings cap is used for encapsulation crystal violet enzyme labeling thing; Brown plastics reagent bottle (6) is used for encapsulation 12ml colour developing liquid, and the white translucent plastic reagent bottle (7) of yellow mark cap is used for encapsulation 12ml stop buffer, and the brown glass reagent bottle (8) of white cap is used for the standard solution of encapsulation 2ml/ bottle; Polyfoam mould (9) has 10 bottle positions, placement location is followed successively by: 60ml sample diluting liquid bottle position (15), 8ml enzyme labeling thing bottle position (10), the 12ml liquid bottle position (11) of developing the color, 12ml stop buffer bottle position (12), the standard solution bottle position (13) of 6 bottles of various concentration of 2ml/ bottle, box body is carton box for (14).

Claims

1. crystal violet enzyme-linked immunologic detecting kit, the recessed bottle position that comprises ELISA Plate, 10 bottles of reagent in 96 holes in box body and the box and put reagent, it is characterized in that: ELISA Plate is to adopt 96 hole agent plate as solid phase carrier, on the kit capillary strip, wrap by the check-out console of mouse-anti crystal violet in advance, described 10 bottles of reagent are respectively 6 bottles of standard solutions, 1 bottle of crystal violet enzyme labeling thing, 1 bottle of colour developing liquid, 1 bottle of stop buffer, 1 bottle of sample diluting liquid, and described reagent bottle all is provided with corresponding recessed bottle position.

2. according to the described kit of claim l, it is characterized in that: described box body is carton box; The ELISA Plate in described 96 holes is the polystyrene ELISA Plate, is positioned in the vacuum aluminium foil bag; Described standard solution is all used the Brown Glass Brown glass bottles and jars only of white cap, the enzyme labeling thing white translucent PE plastic bottle of the white cap of blue markings, colour developing liquid is with brown PE plastic bottle, stop buffer is with the translucent white PE plastic bottle of the white cap of yellow mark, sample diluting liquid is made by plastic foam with the translucent PE plastic bottle of white cap, described recessed bottle position.

3. according to the described kit of claim l, it is characterized in that: described ELISA Plate is made up of outer frame support and removable 12 enzymes mark reaction capillary strips of being placed on it, each dismantled and assembled enzyme mark reaction capillary strip has 8 reacting holes, and each reacting hole wraps in advance by the antibody of mouse-anti crystal violet; The capacity of described standard solution reagent bottle is the 2ml/ bottle; The capacity of enzyme labeling thing reagent bottle is 8ml; The capacity of colour developing liquid reagent bottle is 12ml; The capacity of stop buffer reagent bottle is 12ml; The capacity of sample diluting liquid reagent bottle is 60ml.