CN203981679U - Malachite green vestigial detects ELISA kit - Google Patents
Malachite green vestigial detects ELISA kit Download PDFInfo
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- CN203981679U CN203981679U CN201420336230.0U CN201420336230U CN203981679U CN 203981679 U CN203981679 U CN 203981679U CN 201420336230 U CN201420336230 U CN 201420336230U CN 203981679 U CN203981679 U CN 203981679U
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- malachite green
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- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 title claims abstract description 50
- 229940107698 malachite green Drugs 0.000 title claims abstract description 48
- 238000008157 ELISA kit Methods 0.000 title claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000002965 ELISA Methods 0.000 claims abstract description 17
- 238000010790 dilution Methods 0.000 claims abstract description 17
- 239000012895 dilution Substances 0.000 claims abstract description 17
- 239000012530 fluid Substances 0.000 claims abstract description 15
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- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 6
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- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 6
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- 108090000790 Enzymes Proteins 0.000 claims description 11
- 241000238557 Decapoda Species 0.000 claims description 7
- VOZKAJLKRJDJLL-UHFFFAOYSA-N 2,4-diaminotoluene Chemical compound CC1=CC=C(N)C=C1N VOZKAJLKRJDJLL-UHFFFAOYSA-N 0.000 claims description 5
- 239000013505 freshwater Substances 0.000 claims description 5
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
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- 229920002223 polystyrene Polymers 0.000 claims description 3
- 239000007790 solid phase Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract 1
- 230000002860 competitive effect Effects 0.000 abstract 1
- 229940005654 nitrite ion Drugs 0.000 abstract 1
- 238000004451 qualitative analysis Methods 0.000 abstract 1
- 238000004445 quantitative analysis Methods 0.000 abstract 1
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- 239000000758 substrate Substances 0.000 description 7
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- 238000002835 absorbance Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
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- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- HUMNYLRZRPPJDN-UHFFFAOYSA-N benzaldehyde Chemical compound O=CC1=CC=CC=C1 HUMNYLRZRPPJDN-UHFFFAOYSA-N 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 239000012916 chromogenic reagent Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
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- 108090000765 processed proteins & peptides Proteins 0.000 description 2
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- JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 208000037088 Chromosome Breakage Diseases 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000907663 Siproeta stelenes Species 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000003429 antifungal agent Substances 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
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- 235000021384 green leafy vegetables Nutrition 0.000 description 1
- RNQBLUNNAYFBIW-NPULLEENSA-M hexadecyl(trimethyl)azanium (2S)-2-(6-methoxynaphthalen-2-yl)propanoate Chemical compound COc1ccc2cc(ccc2c1)[C@H](C)C([O-])=O.CCCCCCCCCCCCCCCC[N+](C)(C)C RNQBLUNNAYFBIW-NPULLEENSA-M 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
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- 230000001771 impaired effect Effects 0.000 description 1
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- 210000003205 muscle Anatomy 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
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- 238000012216 screening Methods 0.000 description 1
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- 239000012086 standard solution Substances 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- AAAQKTZKLRYKHR-UHFFFAOYSA-N triphenylmethane Chemical compound C1=CC=CC=C1C(C=1C=CC=CC=1)C1=CC=CC=C1 AAAQKTZKLRYKHR-UHFFFAOYSA-N 0.000 description 1
- 239000002690 trypanocidal agent Substances 0.000 description 1
- 230000000654 trypanocidal effect Effects 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
- 235000005074 zinc chloride Nutrition 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model discloses a kind of malachite green vestigial and detect ELISA kit.Kit comprises box body, lid, is arranged at fixed seat at bottom and top holder in box body, on described fixed seat at bottom, there is the recessed bottle of various reagent bottles position, be used for placing malachite green antibody working fluid, ELIAS secondary antibody working fluid, nitrite ion, stop buffer, concentrated cleaning solution, malachite green titer, matrix dilution and Tween-20 reserve liquid, on described top holder, there are various draw-in grooves or groove, for placing ELISA Plate, capillary strip, suction pipe, colorimetric card and pH test paper.This utility model adopts indirect competitive ELISA method to carry out the qualitative and quantitative analysis of malachite green vestigial, has the advantages such as simple to operate, highly sensitive, with low cost, is applicable to the field quick detection of batch samples.
Description
Technical field
The utility model relates to the detection kit in a kind of medicine bioengineering field, is specifically related to a kind of ELISA detection kit for working sample solution Malachite Green residual content.
Background technology
Malachite green (Malachite Green, MG) have another name called Viride Nitens, aniline green, Victoria green or China green etc., belong to triphenylmethane dye, polymerization under the condition that to be a part benzaldehyde and two molecule xylidins exist at the concentrated sulphuric acid or zinc chloride and the green crystals that forms.The formation of malachite green impede protein peptide in the time of cell division, makes intracellular amino acid cannot be converted into protein peptides, and cell division is suppressed, thereby produces antibacterial insecticidal action.There is people to find that malachite green can make some organize as far back as 1913 painted, destroy pathogen and do not cause that host damages, so malachite green is used as to antiseptic, trypanocides and other medical application.Due to good anti-bacterial effect, low price, malachite green is widely used as pest repellant and germifuge, with the mould in the external parasite of killing aquatic animal, protozoan and fish-egg, and the saprolegniasis of prevention fish, branchiomycosis, ich etc.Sometimes in order to make the impaired fish extending life of squama, deposit in pond in transportation neutralization, also often use malachite green.
But malachite green and metabolic product concealed malachite green thereof be accumulation in vivo easily, is difficult for metabolism.Research is also found: malachite green and concealed malachite green have multiple toxicity, comprises carcinogenicity, causes gene mutation, causes chromosome breakage, teratogenesis tire and respiratory tract toxicity etc.Given this, many countries such as the U.S., Canada, Japan, European Union have all forbidden the antifungal drug as fish by malachite green, and China was also listed malachite green in " veterinary drug and the compound inventory thereof of food animal forbidding " in May, 2002.
Malachite green physics and chemistry detection method comprises gas chromatography (GC), efficient fluidity chromatogram (HPLC), gas chromatography mass spectrometry (GC-MS) and LC-MS (HPLC-MS) etc., these methods are sensitive and accurate, high specificity, degree of separation is good, but need expensive instrument and equipment, sample pre-treatments complexity, loaded down with trivial details time-consuming, testing cost is high, can not execute-in-place, and need professional and technical personnel; By comparison, enzyme-linked immunoassay method has high specificity, highly sensitive, easy and simple to handle, testing cost is low, be suitable for the advantages such as the screening of batch sample, and equipment needed thereby is few, simple to operate easy to learn, with low cost, can meet better China's aquaculture, food processing enterprises, government function supervision department etc. and carry out testing.
Summary of the invention
The malachite green vestigial that the purpose of this utility model has been to provide a kind of small volume and less weight detects ELISA kit, use this kit easy and simple to handle, highly sensitive, cost is low, good stability, can carry out the mensuration of the multiple sample Malachite Green of one-time continuous content, reduce the needed time of sample detection.
The technical solution of the utility model is:
A kind of malachite green vestigial detects ELISA kit, comprise box body and be connected in the lid on box body, the lid of opening one side with on box body, be respectively equipped with corresponding lid dop and box body draw-in groove, it is characterized in that: described box body inside is provided with top holder and fixed seat at bottom from top to bottom successively, and wherein fixed seat at bottom is divided into A by bottom interval, B two parts, A part is provided with 7 the recessed bottle of reagent bottle positions, and B part is provided with 5 the recessed bottle of reagent bottle positions, in the recessed bottle of 7 reagent bottles position of A part, is placed with respectively malachite green antibody working fluid, ELIAS secondary antibody working fluid, developer A liquid, developer B liquid, stop buffer, concentrated cleaning solution and malachite green titer, be placed with respectively fresh-water fishes dilution in the recessed bottle of 5 reagent bottles position of B part, fish dilution, shrimp dilution, feed dilution and Tween-20 reserve liquid, described top holder is divided into A by handle, B two parts, A part is provided with 2 draw-in grooves, and B part is provided with 6 grooves, is placed with respectively 96 hole ELISA Plate and enzyme mark reaction capillary strip in 2 draw-in grooves of A part, in 6 grooves of B part, is placed with respectively 1.0mL suction pipe, 100 μ L micro pipettes, 60 μ L micro pipettes, 50 μ L micro pipettes, colorimetric card and pH test paper are placed with matrix typical curve and instructions above top holder B part.
Described ELISA Plate is made up of polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and be placed in ELISA Plate draw-in groove, and ELISA Plate is made up of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated malachite green coupled antigen on it.
The beneficial effects of the utility model are: the required instrument of kit is less, and operation is simple, and can be fast, accurately, in bulk for sample solution Malachite Green content quantitatively, qualitative detection.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model, and Fig. 2 is the structural representation of fixed seat at bottom in the utility model, and Fig. 3 is the structural representation of top holder in the utility model.
Drawing explanation: 1, box body, 2, lid, 3, fixed seat at bottom, 4, top holder, 5, box body draw-in groove, 6, lid dop, 7, base interval, 8, malachite green antibody working fluid bottle position, 9, ELIAS secondary antibody working fluid bottle position, 10, developer A liquid bottle position, 11, developer B liquid bottle position, 12, stop buffer bottle position, 13, concentrated cleaning solution bottle position, 14, malachite green titer bottle position, 15, fresh-water fishes dilution bottle position, 16, fish dilution bottle position, 17, shrimp dilution bottle position, 18, feed dilution bottle position, 19, Tween-20 reserve liquid bottle position, 20, handle, 21, ELISA Plate draw-in groove, 22, enzyme mark reaction capillary strip draw-in groove, 23, 1.0ml suction pipe groove, 24, 100 μ l micro pipette grooves, 25, 60 μ l micro pipette grooves, 26, 50 μ l micro pipette grooves, 27, colorimetric card groove, 28, pH test paper groove.
Embodiment
Describe by reference to the accompanying drawings embodiment in detail.A kind of malachite green vestigial detects ELISA kit, comprises box body 1, lid 2, fixed seat at bottom 3 and top holder 4 in box body 1 are set, and on described box body 1, has box body draw-in groove 5, corresponding with the lid dop 6 on lid 2, for switch kit.
Described fixed seat at bottom 3 is divided into A, B two parts by bottom interval 7, and A part is provided with 7 the recessed bottle of reagent bottle positions, and B part is provided with 5 the recessed bottle of reagent bottle positions.A part malachite green antibody working fluid bottle position 8 is placed with the 6ml malachite green antibody working fluid of white plastic reagent bottle encapsulation, ELIAS secondary antibody working fluid bottle position 9 is placed with the anti-mouse ELIAS secondary antibody of 6ml rabbit (RaMIgG-HRP) of red plastic reagent bottle encapsulation, developer A liquid bottle position 10 is placed with the 4ml substrate developer A liquid of black plastic reagent bottle encapsulation, developer B liquid bottle position 11 is placed with the 4ml substrate developer B liquid of green plastic reagent bottle encapsulation, stop buffer bottle position 12 is placed with the 7ml 2M sulfuric acid solution of yellow plastic reagent bottle encapsulation, concentrated cleaning solution bottle position 13 is placed with the 40ml concentrated cleaning solution (10 ×) of translucent plastic reagent bottle encapsulation, malachite green titer bottle position 14 is placed with the 2ml malachite green standard solution (1mg/ml) of white transparent plastic reagent bottle encapsulation.B part fresh-water fishes dilution bottle position 15 is placed with the 50ml fresh-water fishes sample diluting liquid of the translucent plastic reagent bottle encapsulation of red cap, fish dilution bottle position 16 is placed with the 50ml fish sample diluting liquid of the translucent plastic reagent bottle encapsulation of yellow cap, shrimp dilution bottle position 17 is placed with the 20ml shrimp sample diluting liquid of the translucent plastic reagent bottle encapsulation of white cap, feed dilution bottle position 18 is placed with the 20ml Feed Sample dilution of the translucent plastic reagent bottle encapsulation of grey cap, Tween-20 reserve liquid bottle position 19 is placed with the 3ml Tween-20 surfactant of the black plastic reagent bottle encapsulation of black caps.
Described top holder 4 is divided into A, B two parts by handle 20, and A part is provided with 2 draw-in grooves, and B part is provided with 6 grooves.The ELISA Plate draw-in groove 21 of A part is placed an ELISA Plate of being made up of polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and ELISA Plate is made up of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated malachite green coupled antigen on it, enzyme mark reaction capillary strip draw-in groove 22 is placed with 24-36 bar enzyme mark reaction in bulk capillary strip, when use, be arranged on the outer frame support of ELISA Plate, make kit measurement capacity expand 2-3 doubly.The 1.0ml suction pipe groove 23 of B part is placed with the suction pipe of 50 1.0ml capacity, for the sampling of sample substrate, 100 μ l micro pipette grooves 24 are placed with the micro pipette of 50 100 μ l capacity, be used for adding stop buffer, 60 μ l micro pipette grooves 25 are placed with the micro pipette of 50 60 μ l capacity, be used for adding chromogenic reagent solution, 50 μ l micro pipette grooves 26 are placed with the micro pipette of 50 50 μ l capacity, be used for adding malachite green antibody working fluid and ELIAS secondary antibody working fluid, colorimetric card groove 27 is placed with the colorimetric card under different substrates condition, show corresponding absorbance and the malachite green vestigial content of different colours reagent wells, for the general reckoning of sample size, pH test paper groove 28 is placed different types of pH test paper, be used for measuring the pH value of different solutions.The surface of B part groove is also placed with matrix typical curve and instructions, and the absorbance substitution typical curve that microplate reader is measured, can Accurate Determining sample solution Malachite Green residual content.
Fish, shrimp Edible tissues Malachite Green assay:
1) sample pre-treatments: get edibility muscle parts in the aquatic products such as fish, shrimp sample, homogenizer homogenate; After accurately taking 2g homogeneous, sample is in 15ml centrifuge tube, then adds 1ml mixed extract (p-toluenesulfonic acid 0.2g, oxammonium hydrochloride 0.1g, ammonium acetate 0.2g, adding distil water dissolves, and is settled to 500ml, be adjusted to pH4.5 with 2mol/L sodium hydroxide solution again) to centrifuge tube, vibration 1min; 3ml acetonitrile is joined in centrifuge tube to thermal agitation 2min; The centrifugal 5min of 4000r/min under room temperature, draws 100 μ l supernatants to be checked.
2) operation steps of kit: 1. required reagent is taken out from cold storage environment, more than being placed in equilibrium at room temperature 20min, note must shaking up before every kind of liquid reagent uses; 2. take out the capillary strip of requirement, no capillary strip is put in former Fresco Bag and together with the drying agent providing and resealed, be stored in 2-8 DEG C; 3. numbering: corresponding sample micropore is numbered according to the order of sequence, and it is parallel that each sample does 2 holes, and record the position at sample well place; 4. add sample: add sample 50 μ l in corresponding micropore, add immediately ELIAS secondary antibody working fluid 50 μ l/holes, then add malachite green antibody working fluid 50 μ l/holes, vibration mixes gently, puts in 37 DEG C of lucifuge environment and reacts 30min; 5. wash plate: liquid in hole is dried, with wash operating solution 300 μ l/ holes, fully wash 5 times, every minor tick 10s, pats dry with thieving paper; 6. colour developing: (l), vibration mixes each 30 μ of A liquid and B liquid gently, puts 37 DEG C of lucifuge environment reaction 15-20min to add substrate chromogenic reagent solution 60 μ l/ holes; 7. measure: add stop buffer 100 μ l/ holes, vibration mixes gently, sets microplate reader in 450nm place, measures every hole absorbance.
3) working sample solution Malachite Green content:
1. qualitative determination: after substrate colour developing 15-20min, by the blue sample well depth and colorimetric card contrast, obtain the residual approximate content of different sample substrate Malachite Greens.
2. quantitative measurement: add behind stop buffer 100 μ l/ holes, the absorbance substitution typical curve that microplate reader is measured, can Accurate Determining sample solution Malachite Green residual content.
Claims (2)
1. a malachite green vestigial detects ELISA kit, comprise box body and be connected in the lid on box body, the lid of opening one side with on box body, be respectively equipped with corresponding lid dop and box body draw-in groove, it is characterized in that: described box body inside is provided with top holder and fixed seat at bottom from top to bottom successively, and wherein fixed seat at bottom is divided into A by bottom interval, B two parts, A part is provided with 7 the recessed bottle of reagent bottle positions, and B part is provided with 5 the recessed bottle of reagent bottle positions, in the recessed bottle of 7 reagent bottles position of A part, is placed with respectively malachite green antibody working fluid, ELIAS secondary antibody working fluid, developer A liquid, developer B liquid, stop buffer, concentrated cleaning solution and malachite green titer, be placed with respectively fresh-water fishes dilution in the recessed bottle of 5 reagent bottles position of B part, fish dilution, shrimp dilution, feed dilution and Tween-20 reserve liquid, described top holder is divided into A by handle, B two parts, A part is provided with 2 draw-in grooves, and B part is provided with 6 grooves, is placed with respectively 96 hole ELISA Plate and enzyme mark reaction capillary strip in 2 draw-in grooves of A part, in 6 grooves of B part, is placed with respectively 1.0mL suction pipe, 100 μ L micro pipettes, 60 μ L micro pipettes, 50 μ L micro pipettes, colorimetric card and pH test paper are placed with matrix typical curve and instructions above top holder B part.
2. malachite green vestigial according to claim 1 detects ELISA kit, it is characterized in that: described ELISA Plate is made up of polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and be placed in ELISA Plate draw-in groove, and ELISA Plate is made up of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, and each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated malachite green coupled antigen on it.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420336230.0U CN203981679U (en) | 2014-06-23 | 2014-06-23 | Malachite green vestigial detects ELISA kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420336230.0U CN203981679U (en) | 2014-06-23 | 2014-06-23 | Malachite green vestigial detects ELISA kit |
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| Publication Number | Publication Date |
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| CN203981679U true CN203981679U (en) | 2014-12-03 |
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| CN201420336230.0U Expired - Fee Related CN203981679U (en) | 2014-06-23 | 2014-06-23 | Malachite green vestigial detects ELISA kit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105954507A (en) * | 2016-04-26 | 2016-09-21 | 北京言必信科技有限公司 | ELISA kit |
| CN107807237A (en) * | 2017-12-13 | 2018-03-16 | 百奥森（江苏）食品安全科技有限公司 | A kind of food inspection kit for being used to detect organophosphor |
-
2014
- 2014-06-23 CN CN201420336230.0U patent/CN203981679U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105954507A (en) * | 2016-04-26 | 2016-09-21 | 北京言必信科技有限公司 | ELISA kit |
| CN107807237A (en) * | 2017-12-13 | 2018-03-16 | 百奥森（江苏）食品安全科技有限公司 | A kind of food inspection kit for being used to detect organophosphor |
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