CN203981681U - Olaquindox metabolite residue detects ELISA kit - Google Patents
Olaquindox metabolite residue detects ELISA kit Download PDFInfo
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- CN203981681U CN203981681U CN201420337323.5U CN201420337323U CN203981681U CN 203981681 U CN203981681 U CN 203981681U CN 201420337323 U CN201420337323 U CN 201420337323U CN 203981681 U CN203981681 U CN 203981681U
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- olaquindox
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- TURHTASYUMWZCC-UHFFFAOYSA-N Olaquindox [BAN:INN] Chemical group C1=CC=C2N([O-])C(C)=C(C(=O)NCCO)[N+](=O)C2=C1 TURHTASYUMWZCC-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 238000008157 ELISA kit Methods 0.000 title claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 229950010210 olaquindox Drugs 0.000 claims abstract description 24
- 238000002965 ELISA Methods 0.000 claims abstract description 17
- 238000010790 dilution Methods 0.000 claims abstract description 17
- 239000012895 dilution Substances 0.000 claims abstract description 17
- 239000012530 fluid Substances 0.000 claims abstract description 16
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims abstract description 14
- 239000000243 solution Substances 0.000 claims abstract description 13
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 6
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims abstract description 6
- 239000011159 matrix material Substances 0.000 claims abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 241000251468 Actinopterygii Species 0.000 claims description 6
- 241000238557 Decapoda Species 0.000 claims description 6
- 235000015277 pork Nutrition 0.000 claims description 6
- VOZKAJLKRJDJLL-UHFFFAOYSA-N 2,4-diaminotoluene Chemical compound CC1=CC=C(N)C=C1N VOZKAJLKRJDJLL-UHFFFAOYSA-N 0.000 claims description 5
- 241000287828 Gallus gallus Species 0.000 claims description 5
- 239000004793 Polystyrene Substances 0.000 claims description 4
- 229920002223 polystyrene Polymers 0.000 claims description 4
- UPUZGXILYFKSGE-UHFFFAOYSA-N quinoxaline-2-carboxylic acid Chemical compound C1=CC=CC2=NC(C(=O)O)=CN=C21 UPUZGXILYFKSGE-UHFFFAOYSA-N 0.000 claims description 4
- 239000000427 antigen Substances 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000007790 solid phase Substances 0.000 claims description 3
- 230000004060 metabolic process Effects 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract 1
- 230000002860 competitive effect Effects 0.000 abstract 1
- 229940005654 nitrite ion Drugs 0.000 abstract 1
- 238000004451 qualitative analysis Methods 0.000 abstract 1
- 238000004445 quantitative analysis Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 17
- 238000005538 encapsulation Methods 0.000 description 12
- 229920003023 plastic Polymers 0.000 description 12
- 239000004033 plastic Substances 0.000 description 11
- 239000000758 substrate Substances 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 5
- 239000012488 sample solution Substances 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- BJPNADFNSANIPF-UHFFFAOYSA-N 3-methylquinoxaline-2-carboxylic acid Chemical compound C1=CC=C2N=C(C(O)=O)C(C)=NC2=C1 BJPNADFNSANIPF-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000012916 chromogenic reagent Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 231100000703 Maximum Residue Limit Toxicity 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of olaquindox metabolite residue and detect ELISA kit.Kit comprises box body, lid, is arranged at fixed seat at bottom and top holder in box body, on described fixed seat at bottom, there is the recessed bottle of various reagent bottles position, be used for placing olaquindox metabolin antibody working fluid, ELIAS secondary antibody working fluid, nitrite ion, stop buffer, concentrated cleaning solution, olaquindox metabolin titer, matrix dilution and Tween-20 reserve liquid, on described top holder, there are various draw-in grooves or groove, for placing ELISA Plate, capillary strip, suction pipe, colorimetric card and pH test paper.This utility model adopts indirect competitive ELISA method to carry out the qualitative and quantitative analysis of olaquindox metabolite residue, has the advantages such as simple to operate, highly sensitive, with low cost, is applicable to the field quick detection of batch samples.
Description
Technical field
The utility model relates to the detection kit in a kind of medicine bioengineering field, is specifically related to a kind of ELISA detection kit for working sample solution olaquindox metabolite residue.
Background technology
Olaquindox (Olaquindox) claim again olaquindox, Olaquindox, early 1970s to develop synthetic feed addictive by West Germany's Baeyer pharmaceutical factory, because it has broad-spectrum antimicrobial effect and growth promoting function, be widely used in the control and prevention of disease of livestock and poultry cultivation, antibacterial therapy and aquatic livestock.Yet large number of biological toxicity test shows: excessive olaquindox can be residual in animal body, has obvious cumulative toxicity, the toxic and side effect such as carcinogenic, aberration inducing effect and DNA damage.In view of the harm that human health is caused, the EC2788/98 of EU Committee resolution is classified olaquindox as forbidding medicine, also stipulates olaquindox forbidding fishing medicine in domestic NY5070-2002 criterion.But olaquindox itself is unstable, can become ten multi-products by short time intracellular metabolite in animal body, wherein 3-Jia based quinoxaline-2-carboxylic acid (MQCA) is one of major metabolite, it is relatively stable in vivo, one of residual marker of international food code council identification, so the common object using olaquindox metabolin MQCA as retention analysis and monitoring.China Ministry of Agriculture in 2003 has stipulated that the maximum residue limit of MQCA in muscle and liver organization is respectively 4 μ g/kg and 50 μ g/kg.
The method for detecting residue of olaquindox and metabolin thereof mainly comprises thin-layered chromatography, high performance liquid chromatography, mass spectroscopy, high efficiency liquid phase-MS etc., these methods are sensitive and accurate, high specificity, degree of separation is good, but need expensive instrument and equipment, sample pre-treatments is complicated, loaded down with trivial details time-consuming, testing cost is high, can not execute-in-place, and need professional and technical personnel; By comparison, enzyme-linked immunoassay method has high specificity, highly sensitive, easy and simple to handle, testing cost is low, be suitable for the advantages such as screening of batch sample, and equipment needed thereby is few, simple to operate easy to learn, with low cost, can meet better China's aquaculture, food processing enterprises, medicament residue supervision department etc. and carry out testing.
Summary of the invention
The olaquindox metabolite residue that the purpose of this utility model has been to provide a kind of small volume and less weight detects ELISA kit, use this kit easy and simple to handle, highly sensitive, cost is low, good stability, can carry out the mensuration of olaquindox metabolite content in a plurality of samples of one-time continuous, reduce the needed time of sample detection.
The technical solution of the utility model is:
A kind of olaquindox metabolite residue detects ELISA kit, comprise box body and be connected in the lid on box body, the lid of opening one side with on box body, be respectively equipped with corresponding lid dop and box body draw-in groove, it is characterized in that: described box body inside is provided with top holder and fixed seat at bottom from top to bottom successively, and wherein fixed seat at bottom is divided into A by bottom interval, B two parts, A is partly provided with 7 the recessed bottle of reagent bottle positions, and B is partly provided with 5 the recessed bottle of reagent bottle positions, in the recessed bottle of 7 reagent bottles position of A part, is placed with respectively olaquindox metabolin antibody working fluid, ELIAS secondary antibody working fluid, developer A liquid, developer B liquid, stop buffer, concentrated cleaning solution and olaquindox metabolism titer, be placed with respectively chicken dilution in the recessed bottle of 5 reagent bottles position of B part, pork dilution, flesh of fish dilution, shrimp dilution and Tween-20 reserve liquid, described top holder is divided into A by handle, B two parts, A is partly provided with 2 draw-in grooves, and B is partly provided with 6 grooves, is placed with respectively 96 hole ELISA Plate and enzyme mark reaction capillary strip in 2 draw-in grooves of A part, in 6 grooves of B part, is placed with respectively 1.0mL suction pipe, 100 μ L micro pipettes, 60 μ L micro pipettes, 50 μ L micro pipettes, colorimetric card and pH test paper are placed with matrix typical curve and instructions above top holder B part.
Described ELISA Plate is made by polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and be placed in ELISA Plate draw-in groove, and ELISA Plate is comprised of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated 3-Jia based quinoxaline-2-carboxylic acid detectable antigens on it.
The beneficial effects of the utility model are: the required instrument of kit is less, and operation is simple, and can be fast, accurately, in bulk for sample solution olaquindox metabolite content quantitatively, qualitative detection.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model, and Fig. 2 is the structural representation of fixed seat at bottom in the utility model, and Fig. 3 is the structural representation of top holder in the utility model.
Drawing explanation: 1, box body, 2, lid, 3, fixed seat at bottom, 4, top holder, 5, box body draw-in groove, 6, lid dop, 7, base interval, 8, olaquindox metabolin antibody working fluid bottle position, 9, ELIAS secondary antibody working fluid bottle position, 10, developer A liquid bottle position, 11, developer B liquid bottle position, 12, stop buffer bottle position, 13, concentrated cleaning solution bottle position, 14, olaquindox metabolin titer bottle position, 15, chicken dilution bottle position, 16, pork dilution bottle position, 17, flesh of fish dilution bottle position, 18, shrimp dilution bottle position, 19, Tween-20 reserve liquid bottle position, 20, handle, 21, ELISA Plate draw-in groove, 22, enzyme mark reaction capillary strip draw-in groove, 23, 1.0ml suction pipe groove, 24, 100 μ l micro pipette grooves, 25, 60 μ l micro pipette grooves, 26, 50 μ l micro pipette grooves, 27, colorimetric card groove, 28, pH test paper groove.
Embodiment
Describe by reference to the accompanying drawings embodiment in detail.A kind of olaquindox metabolite residue detects ELISA kit, comprise box body 1, lid 2, fixed seat at bottom 3 and top holder 4 in box body 1 are set, on described box body 1, there is box body draw-in groove 5, corresponding with the lid dop 6 on lid 2, for switch kit.
Described fixed seat at bottom 3 is divided into A, B two parts by bottom interval 7, and A is partly provided with 7 the recessed bottle of reagent bottle positions, and B is partly provided with 5 the recessed bottle of reagent bottle positions.A part olaquindox metabolin antibody working fluid bottle position 8 is placed with the 6ml olaquindox metabolin antibody working fluid of white plastic reagent bottle encapsulation, ELIAS secondary antibody working fluid bottle position 9 is placed with the anti-mouse ELIAS secondary antibody of 6ml rabbit (RaMIgG-HRP) of red plastic reagent bottle encapsulation, developer A liquid bottle position 10 is placed with the 4ml substrate developer A liquid of black plastic reagent bottle encapsulation, developer B liquid bottle position 11 is placed with the 4ml substrate developer B liquid of green plastic reagent bottle encapsulation, stop buffer bottle position 12 is placed with the 7ml 2M sulfuric acid solution of yellow plastic reagent bottle encapsulation, concentrated cleaning solution bottle position 13 is placed with the 40ml concentrated cleaning solution (10 *) of translucent plastic reagent bottle encapsulation, olaquindox metabolin titer bottle position 14 is placed with the 2ml olaquindox metabolin standard solution (1mg/ml) of white transparent plastic reagent bottle encapsulation.B part chicken dilution bottle position 15 is placed with the 50ml chicken meat sample dilution of the translucent plastic reagent bottle encapsulation of red cap, pork dilution bottle position 16 is placed with the 50ml pork sample diluting liquid of the translucent plastic reagent bottle encapsulation of yellow cap, flesh of fish dilution bottle position 17 is placed with the 20ml flesh of fish sample diluting liquid of the translucent plastic reagent bottle encapsulation of white cap, shrimp dilution bottle position 18 is placed with the 20ml shrimp sample diluting liquid of the translucent plastic reagent bottle encapsulation of grey cap, Tween-20 reserve liquid bottle position 19 is placed with the 3ml Tween-20 surfactant of the black plastic reagent bottle encapsulation of black caps.
Described top holder 4 is divided into A, B two parts by handle 20, and A is partly provided with 2 draw-in grooves, and B is partly provided with 6 grooves.The ELISA Plate draw-in groove 21 of A part is placed an ELISA Plate of being made by polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and ELISA Plate is comprised of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated 3-Jia based quinoxaline-2-carboxylic acid detectable antigens on it, enzyme mark reaction capillary strip draw-in groove 22 is placed with 24-36 bar enzyme mark reaction in bulk capillary strip, during use, be arranged on the outer frame support of ELISA Plate, make kit measurement capacity expand 2-3 doubly.The 1.0ml suction pipe groove 23 of B part is placed with the suction pipe of 50 1.0ml capacity, sampling for sample substrate, 100 μ l micro pipette grooves 24 are placed with the micro pipette of 50 100 μ l capacity, be used for adding stop buffer, 60 μ l micro pipette grooves 25 are placed with the micro pipette of 50 60 μ l capacity, be used for adding chromogenic reagent solution, 50 μ l micro pipette grooves 26 are placed with the micro pipette of 50 50 μ l capacity, be used for adding olaquindox metabolin antibody working fluid and ELIAS secondary antibody working fluid, colorimetric card groove 27 is placed with the colorimetric card under different substrates condition, show corresponding absorbance and the olaquindox metabolite residue content of different colours reagent wells, general reckoning for sample size, pH test paper groove 28 is placed different types of pH test paper, be used for measuring the pH value of different solutions.The surface of B part groove is also placed with matrix typical curve and instructions, the absorbance substitution typical curve that microplate reader is measured, can Accurate Determining sample solution in olaquindox metabolite residue content.
In animal Edible tissues, olaquindox metabolite content is measured:
1) chicken, pork, fish, shrimp sample pre-treatments: take the equal quality sample of 1.00 ± 0.05g to 50ml polystyrene centrifuge tube, add 8ml ethyl acetate, add 1ml deionized water, with the oscillator 5min that vibrates; Add again 2mol/L H
2sO
4solution 1ml, vibration 30s, more than 3000r/min, room temperature (20 ~ 25) ℃ centrifugal 5min; Get 4ml supernatant to the clean glass test tube of 10ml, in 50 ~ 60 ℃ of water-bath nitrogen, flow down and dry up; Add 1ml normal hexane, with the whirling motion of vortex instrument, 30s dissolves dry thing, adds 1ml redissolution liquid working fluid, with vortex instrument whirling motion 2min, and more than 3000r/min, room temperature (20 ~ 25) ℃ centrifugal 5min; Remove upper strata normal hexane phase, take off layer water 50 μ l for analyzing.
2) operation steps of kit: 1. required reagent is taken out from cold storage environment, more than being placed in equilibrium at room temperature 20min, note must shaking up before every kind of liquid reagent is used; 2. take out the capillary strip of requirement, no capillary strip is put in former Fresco Bag and together with the drying agent providing and resealed, be stored in 2-8 ℃; 3. number: the corresponding micropore of sample is numbered according to the order of sequence, and it is parallel that each sample is done 2 holes, and record the position at sample well place; 4. add sample: add sample 50 μ l in corresponding micropore, add immediately ELIAS secondary antibody working fluid 50 μ l/holes, then add olaquindox metabolin antibody working fluid 50 μ l/holes, vibration mixes gently, puts in 37 ℃ of lucifuge environment and reacts 30min; 5. wash plate: liquid in hole is dried, with wash operating solution 300 μ l/ holes, fully wash 5 times, every minor tick 10s, pats dry with thieving paper; 6. colour developing: add substrate chromogenic reagent solution 60 μ l/ holes (each 30 μ l of A liquid and B liquid), vibration mixes gently, puts 37 ℃ of lucifuge environment reaction 15-20min; 7. measure: add stop buffer 100 μ l/ holes, vibration mixes gently, sets microplate reader in 450nm place, measures every hole absorbance.
3) olaquindox metabolite content in working sample solution:
1. qualitative determination: after substrate colour developing 15-20min, by the blue depth of sample well and colorimetric card contrast, obtain olaquindox metabolite residue approximate content in different sample substrates.
2. quantitative measurement: add behind stop buffer 100 μ l/ holes, the absorbance substitution typical curve that microplate reader is measured, can Accurate Determining sample solution in olaquindox metabolite residue content.
Claims (2)
1. an olaquindox metabolite residue detects ELISA kit, comprise box body and be connected in the lid on box body, the lid of opening one side with on box body, be respectively equipped with corresponding lid dop and box body draw-in groove, it is characterized in that: described box body inside is provided with top holder and fixed seat at bottom from top to bottom successively, and wherein fixed seat at bottom is divided into A by bottom interval, B two parts, A is partly provided with 7 the recessed bottle of reagent bottle positions, and B is partly provided with 5 the recessed bottle of reagent bottle positions, in the recessed bottle of 7 reagent bottles position of A part, is placed with respectively olaquindox metabolin antibody working fluid, ELIAS secondary antibody working fluid, developer A liquid, developer B liquid, stop buffer, concentrated cleaning solution and olaquindox metabolism titer, be placed with respectively chicken dilution in the recessed bottle of 5 reagent bottles position of B part, pork dilution, flesh of fish dilution, shrimp dilution and Tween-20 reserve liquid, described top holder is divided into A by handle, B two parts, A is partly provided with 2 draw-in grooves, and B is partly provided with 6 grooves, is placed with respectively 96 hole ELISA Plate and enzyme mark reaction capillary strip in 2 draw-in grooves of A part, in 6 grooves of B part, is placed with respectively 1.0mL suction pipe, 100 μ L micro pipettes, 60 μ L micro pipettes, 50 μ L micro pipettes, colorimetric card and pH test paper are placed with matrix typical curve and instructions above top holder B part.
2. olaquindox metabolite residue according to claim 1 detects ELISA kit, it is characterized in that: described ELISA Plate is made by polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and be placed in ELISA Plate draw-in groove, and ELISA Plate is comprised of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, and each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated 3-first based quinoxaline-2-carboxylic acid detectable antigens on it.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420337323.5U CN203981681U (en) | 2014-06-23 | 2014-06-23 | Olaquindox metabolite residue detects ELISA kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420337323.5U CN203981681U (en) | 2014-06-23 | 2014-06-23 | Olaquindox metabolite residue detects ELISA kit |
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| Publication Number | Publication Date |
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| CN203981681U true CN203981681U (en) | 2014-12-03 |
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| CN201420337323.5U Expired - Fee Related CN203981681U (en) | 2014-06-23 | 2014-06-23 | Olaquindox metabolite residue detects ELISA kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104597178A (en) * | 2015-01-14 | 2015-05-06 | 华中农业大学 | 3-methylquinoxaline-2-carboxylic acid immunoaffinity column and preparation method thereof |
-
2014
- 2014-06-23 CN CN201420337323.5U patent/CN203981681U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104597178A (en) * | 2015-01-14 | 2015-05-06 | 华中农业大学 | 3-methylquinoxaline-2-carboxylic acid immunoaffinity column and preparation method thereof |
| CN104597178B (en) * | 2015-01-14 | 2016-05-11 | 华中农业大学 | A kind of 3-Jia based quinoxaline-2 carboxylic acid immune affinity column and preparation method thereof |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141203 |
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| CF01 | Termination of patent right due to non-payment of annual fee |