CN203981685U - A kind of immunological detecting kit of tetracycline - Google Patents
A kind of immunological detecting kit of tetracycline Download PDFInfo
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- CN203981685U CN203981685U CN201420336227.9U CN201420336227U CN203981685U CN 203981685 U CN203981685 U CN 203981685U CN 201420336227 U CN201420336227 U CN 201420336227U CN 203981685 U CN203981685 U CN 203981685U
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- 239000004098 Tetracycline Substances 0.000 title claims abstract description 43
- 235000019364 tetracycline Nutrition 0.000 title claims abstract description 43
- 150000003522 tetracyclines Chemical class 0.000 title claims abstract description 43
- 229960002180 tetracycline Drugs 0.000 title claims abstract description 42
- 229930101283 tetracycline Natural products 0.000 title claims abstract description 42
- 230000001900 immune effect Effects 0.000 title claims abstract description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 239000012895 dilution Substances 0.000 claims abstract description 18
- 238000010790 dilution Methods 0.000 claims abstract description 18
- 239000012530 fluid Substances 0.000 claims abstract description 16
- 238000002965 ELISA Methods 0.000 claims abstract description 15
- 239000000243 solution Substances 0.000 claims abstract description 14
- 238000012360 testing method Methods 0.000 claims abstract description 7
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- 239000011159 matrix material Substances 0.000 claims abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 210000004185 liver Anatomy 0.000 claims description 7
- 235000013336 milk Nutrition 0.000 claims description 7
- 239000008267 milk Substances 0.000 claims description 7
- 210000004080 milk Anatomy 0.000 claims description 7
- 210000003205 muscle Anatomy 0.000 claims description 7
- VOZKAJLKRJDJLL-UHFFFAOYSA-N 2,4-diaminotoluene Chemical compound CC1=CC=C(N)C=C1N VOZKAJLKRJDJLL-UHFFFAOYSA-N 0.000 claims description 5
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
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- 238000000576 coating method Methods 0.000 claims description 3
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- 239000007790 solid phase Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 3
- 230000002860 competitive effect Effects 0.000 abstract description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract 1
- 229940005654 nitrite ion Drugs 0.000 abstract 1
- 238000004451 qualitative analysis Methods 0.000 abstract 1
- 238000004445 quantitative analysis Methods 0.000 abstract 1
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- 244000144972 livestock Species 0.000 description 3
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- 230000003115 biocidal effect Effects 0.000 description 2
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- SGKRLCUYIXIAHR-AKNGSSGZSA-N (4s,4ar,5s,5ar,6r,12ar)-4-(dimethylamino)-1,5,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4a,5,5a,6-tetrahydro-4h-tetracene-2-carboxamide Chemical compound C1=CC=C2[C@H](C)[C@@H]([C@H](O)[C@@H]3[C@](C(O)=C(C(N)=O)C(=O)[C@H]3N(C)C)(O)C3=O)C3=C(O)C2=C1O SGKRLCUYIXIAHR-AKNGSSGZSA-N 0.000 description 1
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- 241000894006 Bacteria Species 0.000 description 1
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- KXDHJXZQYSOELW-UHFFFAOYSA-N Carbamic acid Chemical compound NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 231100000703 Maximum Residue Limit Toxicity 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
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- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- KIPLYOUQVMMOHB-MXWBXKMOSA-L [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O Chemical compound [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O KIPLYOUQVMMOHB-MXWBXKMOSA-L 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
- 210000003445 biliary tract Anatomy 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000004976 chemiluminescence spectroscopy Methods 0.000 description 1
- CYDMQBQPVICBEU-UHFFFAOYSA-N chlorotetracycline Natural products C1=CC(Cl)=C2C(O)(C)C3CC4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-UHFFFAOYSA-N 0.000 description 1
- 229960004475 chlortetracycline Drugs 0.000 description 1
- CYDMQBQPVICBEU-XRNKAMNCSA-N chlortetracycline Chemical compound C1=CC(Cl)=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-XRNKAMNCSA-N 0.000 description 1
- 235000019365 chlortetracycline Nutrition 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 229960003722 doxycycline Drugs 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 235000020251 goat milk Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229960004023 minocycline Drugs 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 229940063650 terramycin Drugs 0.000 description 1
- 229940040944 tetracyclines Drugs 0.000 description 1
- 210000001635 urinary tract Anatomy 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a kind of immunological detecting kit of tetracycline.Kit comprises box body, lid, is arranged at fixed seat at bottom and top holder in box body, on described fixed seat at bottom, there is the recessed bottle of various reagent bottles position, be used for placing tetracycline antibody working fluid, ELIAS secondary antibody working fluid, nitrite ion, stop buffer, concentrated cleaning solution, tetracycline titer, matrix dilution and Tween-20 reserve liquid, on described top holder, there are various draw-in grooves or groove, for placing ELISA Plate, capillary strip, suction pipe, colorimetric card and pH test paper.This utility model adopts indirect competitive ELISA method to carry out the qualitative and quantitative analysis of tetracycline content, has the advantages such as simple to operate, highly sensitive, with low cost, is applicable to the field quick detection of batch samples.
Description
Technical field
The utility model relates to the detection kit in a kind of medicine bioengineering field, is specifically related to a kind of immunological detecting kit for working sample solution tetracycline residual content.
Background technology
Tetracycline medication (Tetracycline, TCs) be the class alkalescence broad-spectrum antibiotic being produced by streptomycete, in chemical constitution, belong to the derivant of many ring aphthacene Carboxylamide parent nucleus, comprise aureomycin, terramycin, tetracycline and demethylchlortetra cylinum, and semisynthetic Doxycycline and minocycline etc.Such medicine can be combined with the A position of bacterial ribosome 30S subunit specifically, stop aminoacyl-tRNA in this locational connection, thereby suppress peptide chain growth, affect bacterioprotein synthesize.Tetracycline medication is mainly used in the non-bacterial infections such as Li Keshi body, Chlamydia, mycoplasma and Spirochaeta recurrentis, and treats the multi-infection disease at the positions such as responsive microbial respiratory tract, biliary tract, urinary tract and skin soft tissue.In Production of Livestock and Poultry, tetracycline medication is extensively used as medicine and medicated premix, for preventing and treating infection of digestive canal, promote growth and improving milk production of cow.
Along with TCs quilt is a large amount of, be widely used in livestock culture industry, its food residue causing brings great potential safety hazard to human physical and mental health.People takes in TCs for a long time can cause the various chronic and property accumulated diseases, main manifestations is the infringement to stomach, intestines, liver, and the dyeing of tooth, also can cause allergic reaction, suprainfection, teratogenesis tire etc., also can cause the imbalance of colony balance and the generation of antibody-resistant bacterium in human body intestinal canal simultaneously.Therefore, countries in the world are extremely paid close attention to TCs residue detection.Japan requires telracycline family residual quantity in import livestock and poultry meat must not exceed 0.05mg/kg, Australia's regulation Tetracyclines total amount must not be greater than 0.3mg/kg, European Union's uniform limit is 0.01mg/kg, the regulation TCs of united states drug Surveillance Authority residual total amount in animal muscle tissue is no more than 0.02mg/kg, is 0.3mg/kg in milk.The Ministry of Agriculture of China has revised " animal food herbal medicine maximum residue limit(MRL) " in 2002, wherein in regulation ox/goat milk and all animal muscles, the high residue amount of TCs is 0.1mg/kg, also has a phase limes reacting dose regulation to tetracycline in animal's liver, kidney, egg is residual.
In food, teracycline antibiotic residues detection method has high performance liquid chromatography, capillary electrophoresis, electrochemical analysis, chemiluminescence and Liquid Chromatography-Mass Spectrometry etc., these methods require high to the pre-treatment of testing sample, and operate loaded down with trivial detailsly, need accurate instrument and equipment.And the feature such as that immunological method has is quick, sensitive, high specificity is one of effective ways of detection of veterinary drugs in food.Therefore, develop quick, sensitive, efficient tetracycline residue detection indirect competitive ELISA kit for ensureing that animal food safety tool is of great significance.
Summary of the invention
The purpose of this utility model has been to provide a kind of immunological detecting kit of tetracycline of small volume and less weight, uses this kit easy and simple to handle, highly sensitive, cost is low, good stability, can carry out the mensuration of tetracycline content in the multiple samples of one-time continuous, has reduced the needed time of sample detection.
The technical solution of the utility model is:
A kind of immunological detecting kit of tetracycline, comprise box body and be connected in the lid on box body, the lid of opening one side with on box body, be respectively equipped with corresponding lid dop and box body draw-in groove, it is characterized in that: described box body inside is provided with top holder and fixed seat at bottom from top to bottom successively, and wherein fixed seat at bottom is divided into A by bottom interval, B two parts, A part is provided with 7 the recessed bottle of reagent bottle positions, and B part is provided with 5 the recessed bottle of reagent bottle positions, in the recessed bottle of 7 reagent bottles position of A part, is placed with respectively tetracycline antibody working fluid, ELIAS secondary antibody working fluid, developer A liquid, developer B liquid, stop buffer, concentrated cleaning solution and tetracycline titer, be placed with respectively muscle dilution in the recessed bottle of 5 reagent bottles position of B part, liver dilution, milk dilution, feed dilution and Tween-20 reserve liquid, described top holder is divided into A by handle, B two parts, A part is provided with 2 draw-in grooves, and B part is provided with 6 grooves, is placed with respectively 96 hole ELISA Plate and enzyme mark reaction capillary strip in 2 draw-in grooves of A part, in 6 grooves of B part, is placed with respectively 1.0mL suction pipe, 100 μ L micro pipettes, 60 μ L micro pipettes, 50 μ L micro pipettes, colorimetric card and pH test paper are placed with matrix typical curve and instructions above top holder B part.
Described ELISA Plate is made up of polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and be placed in ELISA Plate draw-in groove, and ELISA Plate is made up of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated tetracycline coupled antigen on it.
The beneficial effects of the utility model are: the required instrument of kit is less, and operation is simple, and can be fast, accurately, in bulk for sample solution tetracycline content quantitatively, qualitative detection.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model, and Fig. 2 is the structural representation of fixed seat at bottom in the utility model, and Fig. 3 is the structural representation of top holder in the utility model.
Drawing explanation: 1, box body, 2, lid, 3, fixed seat at bottom, 4, top holder, 5, box body draw-in groove, 6, lid dop, 7, base interval, 8, tetracycline antibody working fluid bottle position, 9, ELIAS secondary antibody working fluid bottle position, 10, developer A liquid bottle position, 11, developer B liquid bottle position, 12, stop buffer bottle position, 13, concentrated cleaning solution bottle position, 14, tetracycline titer bottle position, 15, muscle dilution bottle position, 16, liver dilution bottle position, 17, milk dilution bottle position, 18, feed dilution bottle position, 19, Tween-20 reserve liquid bottle position, 20, handle, 21, ELISA Plate draw-in groove, 22, enzyme mark reaction capillary strip draw-in groove, 23, 1.0ml suction pipe groove, 24, 100 μ l micro pipette grooves, 25, 60 μ l micro pipette grooves, 26, 50 μ l micro pipette grooves, 27, colorimetric card groove, 28, pH test paper groove.
Embodiment
Describe by reference to the accompanying drawings embodiment in detail.An immunological detecting kit for tetracycline, comprises box body 1, lid 2, fixed seat at bottom 3 and top holder 4 in box body 1 is set, and on described box body 1, has box body draw-in groove 5, corresponding with the lid dop 6 on lid 2, for switch kit.
Described fixed seat at bottom 3 is divided into A, B two parts by bottom interval 7, and A part is provided with 7 the recessed bottle of reagent bottle positions, and B part is provided with 5 the recessed bottle of reagent bottle positions.A part tetracycline antibody working fluid bottle position 8 is placed with the 6ml tetracycline antibody working fluid of white plastic reagent bottle encapsulation, ELIAS secondary antibody working fluid bottle position 9 is placed with the anti-mouse ELIAS secondary antibody of 6ml rabbit (RaMIgG-HRP) of red plastic reagent bottle encapsulation, developer A liquid bottle position 10 is placed with the 4ml substrate developer A liquid of black plastic reagent bottle encapsulation, developer B liquid bottle position 11 is placed with the 4ml substrate developer B liquid of green plastic reagent bottle encapsulation, stop buffer bottle position 12 is placed with the 7ml 2M sulfuric acid solution of yellow plastic reagent bottle encapsulation, concentrated cleaning solution bottle position 13 is placed with the 40ml concentrated cleaning solution (10 ×) of translucent plastic reagent bottle encapsulation, tetracycline titer bottle position 14 is placed with the 2ml tetracycline standard solution (1mg/ml) of white transparent plastic reagent bottle encapsulation.B part muscle dilution bottle position 15 is placed with the 50ml muscle samples dilution of the translucent plastic reagent bottle encapsulation of red cap, liver dilution bottle position 16 is placed with the 50ml liver sample diluting liquid of the translucent plastic reagent bottle encapsulation of yellow cap, milk dilution bottle position 17 is placed with the 20ml milk sample dilution of the translucent plastic reagent bottle encapsulation of white cap, feed dilution bottle position 18 is placed with the 20ml Feed Sample dilution of the translucent plastic reagent bottle encapsulation of grey cap, Tween-20 reserve liquid bottle position 19 is placed with the 3ml Tween-20 surfactant of the black plastic reagent bottle encapsulation of black caps.
Described top holder 4 is divided into A, B two parts by handle 20, and A part is provided with 2 draw-in grooves, and B part is provided with 6 grooves.The ELISA Plate draw-in groove 21 of A part is placed an ELISA Plate of being made up of polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and ELISA Plate is made up of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated tetracycline coupled antigen on it, enzyme mark reaction capillary strip draw-in groove 22 is placed with 24-36 bar enzyme mark reaction in bulk capillary strip, when use, be arranged on the outer frame support of ELISA Plate, make kit measurement capacity expand 2-3 doubly.The 1.0ml suction pipe groove 23 of B part is placed with the suction pipe of 50 1.0ml capacity, for the sampling of sample substrate, 100 μ l micro pipette grooves 24 are placed with the micro pipette of 50 100 μ l capacity, be used for adding stop buffer, 60 μ l micro pipette grooves 25 are placed with the micro pipette of 50 60 μ l capacity, be used for adding chromogenic reagent solution, 50 μ l micro pipette grooves 26 are placed with the micro pipette of 50 50 μ l capacity, be used for adding tetracycline antibody working fluid and ELIAS secondary antibody working fluid, colorimetric card groove 27 is placed with the colorimetric card under different substrates condition, show corresponding absorbance and the tetracycline residual content of different colours reagent wells, for the general reckoning of sample size, pH test paper groove 28 is placed different types of pH test paper, be used for measuring the pH value of different solutions.The surface of B part groove is also placed with matrix typical curve and instructions, the absorbance substitution typical curve that microplate reader is measured, can Accurate Determining sample solution in tetracycline residual content.
Tetracycline assay in animal Edible tissues:
1) Sample pretreatment: take in chopping, the sample 1.0 ± 0.05g to 5ml of animal tissue centrifuge tube after homogeneous, add the Mcllvaine-EDTA sample extracting solution of 2ml pH4.0, whirlpool mixes 3～5min, the centrifugal 5min of 4000r/min under room temperature; Pipette 1ml supernatant in 5ml centrifuge tube, add 50 μ l 4mol/L NaOH, vibration mixes; Add again 1ml normal hexane, whirlpool mixing 30s; Leave standstill after 5min layering, draw at least 100 μ l lower floor solution and mix rear to be checked with equal-volume PBS damping fluid.
2) operation steps of kit: 1. required reagent is taken out from cold storage environment, more than being placed in equilibrium at room temperature 20min, note must shaking up before every kind of liquid reagent uses; 2. take out the capillary strip of requirement, no capillary strip is put in former Fresco Bag and together with the drying agent providing and resealed, be stored in 2-8 DEG C; 3. numbering: corresponding sample micropore is numbered according to the order of sequence, and it is parallel that each sample does 2 holes, and record the position at sample well place; 4. add sample: add sample 50 μ l in corresponding micropore, add immediately ELIAS secondary antibody working fluid 50 μ l/holes, then add tetracycline antibody working fluid 50 μ l/holes, vibration mixes gently, puts in 37 DEG C of lucifuge environment and reacts 30min; 5. wash plate: liquid in hole is dried, with wash operating solution 300 μ l/ holes, fully wash 5 times, every minor tick 10s, pats dry with thieving paper; 6. colour developing: (l), vibration mixes each 30 μ of A liquid and B liquid gently, puts 37 DEG C of lucifuge environment reaction 15-20min to add substrate chromogenic reagent solution 60 μ l/ holes; 7. measure: add stop buffer 100 μ l/ holes, vibration mixes gently, sets microplate reader in 450nm place, measures every hole absorbance.
3) tetracycline content in working sample solution:
1. qualitative determination: after substrate colour developing 15-20min, by the blue sample well depth and colorimetric card contrast, obtain the residual approximate content of tetracycline in different sample substrates.
2. quantitative measurement: add behind stop buffer 100 μ l/ holes, the absorbance substitution typical curve that microplate reader is measured, can Accurate Determining sample solution in tetracycline residual content.
Claims (2)
1. the immunological detecting kit of a tetracycline, comprise box body and be connected in the lid on box body, the lid of opening one side with on box body, be respectively equipped with corresponding lid dop and box body draw-in groove, it is characterized in that: described box body inside is provided with top holder and fixed seat at bottom from top to bottom successively, and wherein fixed seat at bottom is divided into A by bottom interval, B two parts, A part is provided with 7 the recessed bottle of reagent bottle positions, and B part is provided with 5 the recessed bottle of reagent bottle positions, in the recessed bottle of 7 reagent bottles position of A part, is placed with respectively tetracycline antibody working fluid, ELIAS secondary antibody working fluid, developer A liquid, developer B liquid, stop buffer, concentrated cleaning solution and tetracycline titer, be placed with respectively muscle dilution in the recessed bottle of 5 reagent bottles position of B part, liver dilution, milk dilution, feed dilution and Tween-20 reserve liquid, described top holder is divided into A by handle, B two parts, A part is provided with 2 draw-in grooves, and B part is provided with 6 grooves, is placed with respectively 96 hole ELISA Plate and enzyme mark reaction capillary strip in 2 draw-in grooves of A part, in 6 grooves of B part, is placed with respectively 1.0mL suction pipe, 100 μ L micro pipettes, 60 μ L micro pipettes, 50 μ L micro pipettes, colorimetric card and pH test paper are placed with matrix typical curve and instructions above top holder B part.
2. the immunological detecting kit of tetracycline according to claim 1, it is characterized in that: described ELISA Plate is made up of polystyrene, adopt 96 hole agent plate as solid phase carrier, be put in aluminide-coating bag, and be placed in ELISA Plate draw-in groove, and ELISA Plate is made up of outer frame support and the dismantled and assembled enzyme mark reaction capillary strip being placed on it, and each dismantled and assembled enzyme mark reaction capillary strip has 12 reacting holes, pre-coated tetracycline coupled antigen on it.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420336227.9U CN203981685U (en) | 2014-06-23 | 2014-06-23 | A kind of immunological detecting kit of tetracycline |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420336227.9U CN203981685U (en) | 2014-06-23 | 2014-06-23 | A kind of immunological detecting kit of tetracycline |
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| Publication Number | Publication Date |
|---|---|
| CN203981685U true CN203981685U (en) | 2014-12-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420336227.9U Expired - Fee Related CN203981685U (en) | 2014-06-23 | 2014-06-23 | A kind of immunological detecting kit of tetracycline |
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| Country | Link |
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| CN (1) | CN203981685U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771263A (en) * | 2016-12-01 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | Methyltestosterone detection method and kit in a kind of chicken |
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2014
- 2014-06-23 CN CN201420336227.9U patent/CN203981685U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771263A (en) * | 2016-12-01 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | Methyltestosterone detection method and kit in a kind of chicken |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20141203 |