CN107807237A - A kind of food inspection kit for being used to detect organophosphor - Google Patents
A kind of food inspection kit for being used to detect organophosphor Download PDFInfo
- Publication number
- CN107807237A CN107807237A CN201711324557.0A CN201711324557A CN107807237A CN 107807237 A CN107807237 A CN 107807237A CN 201711324557 A CN201711324557 A CN 201711324557A CN 107807237 A CN107807237 A CN 107807237A
- Authority
- CN
- China
- Prior art keywords
- organophosphor
- buffer solution
- liquid
- detect
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of food inspection kit for being used to detect organophosphor, including box body, the box body leading flank bottom is provided with ELISA Plate room, the side of the box body is provided with drop rifle room, the tray interior is set side by side with standard testing strips room and reative cell, the reaction chamber surface is provided with drip hole, the standard testing strips room and the top of reative cell set abacus, the putting hole of solution reagent bottle is provided with the orifice plate, the putting hole includes coating buffer solution, conventional liquid, confining liquid, cleaning solution, buffer solution, ELIAS secondary antibody buffer solution, nitrite ion, terminate liquid, realize the experimental reaction of primary of kit, ensure accuracy when solution uses, ELISA Plate and drop rifle are integrated into kit, increase its universality.
Description
Technical field
The present invention relates to organophosphor detection technique field, specially a kind of food inspection reagent for being used to detect organophosphor
Box.
Background technology
Organophosphorus pesticide is mostly phosphoric acid ester or group thiophosphate compound, has various in style, strong drug action and desinsection
Compose the features such as wide, 70-eighties of 20th century, such medicine is widely used in the prevention and control of plant diseases, pest control in agricultural production, afterwards because of its poison
Property height largely disabled, wherein, in China's disabling comprehensively, fenifrothion has been big in European Union and Australia for parathion, parathion-methyl
Leah disables, with the improvement of living standards and environmental consciousness enhancing, people pay attention to Pesticide Residue further, establish simultaneously
Improve residues of pesticides detection and supervision mechanism demand it is growing day by day, organophosphorus pesticide analysis method mainly includes：It is high
Effect liquid phase chromatogram method, gas chromatography, gas chromatography tandem mass spectrometry method, these analysis methods are because with efficient, sensitive and accurate
The features such as be widely used, it is but higher to equipment and technical requirements, and sample pretreatment process is complicated, is not suitable on a large scale
Quick screening.
The detection for residues of pesticides all rests on the stage that can only once detect a sample mostly at present, so
Once sample size is a lot, it must many times be detected, not only reduce detection efficiency, also reduce the accuracy of detection.
In the prior art scheme, Application No. 201320068623.3, patent name are surveyed for high flux organophosphorus pesticide
Box is tried, the technical solution that the patent provides is that testing cassete box body is respectively arranged with support frame and packaging bag from the bottom up, institute
State and be embedded with eluent bottle, developer bottle and substrate bottle in support frame respectively, ELISA Plate is provided with packaging bag.And only by enzyme mark
The detection of plate can not be effectively accomplished preferable measurement data, can only draw depositing for single phosphorus content from simple reaction
Lacking accuracy, and easily disturbed by environmental factor by target detection.
The content of the invention
In order to overcome the shortcomings of prior art, the present invention provides a kind of food inspection reagent for being used to detect organophosphor
Box, the experimental reaction of primary of kit is realized, ensures accuracy when solution uses, ELISA Plate and drop rifle are integrated into reagent
In box, increase its universality, effectively can solve the problems, such as in background technology.
The technical solution adopted for the present invention to solve the technical problems is：A kind of food inspection examination for being used to detect organophosphor
Agent box, including box body, the box body leading flank bottom are provided with ELISA Plate room, and the side of the box body is provided with drop rifle room, institute
State tray interior and be set side by side with standard testing strips room and reative cell, the reaction chamber surface is provided with drip hole, the standard testing strips
Room and the top of reative cell set abacus, and the putting hole of solution reagent bottle is provided with the orifice plate, and the putting hole includes
It is coated with buffer solution, conventional liquid, confining liquid, cleaning solution, buffer solution, ELIAS secondary antibody buffer solution, nitrite ion, terminate liquid.
As a kind of preferable technical scheme of the present invention, the coating buffer solution is 0.05mol/L, pH9.6 carbonate
Buffer solution, the conventional liquid use 0.01mol/L, pH7.4 phosphate buffer, and the buffer solution uses 0.1mol/L,
PH8.5 phosphate buffer, the ELIAS secondary antibody buffer solution use PBS of the mass fraction for 2% skimmed milk power.
As a kind of preferable technical scheme of the present invention, the solution that confining liquid and the ELIAS secondary antibody buffer solution uses
Together, the cleaning solution uses the PBS containing 0.05%Tween-20, and the nitrite ion uses adjacent benzene disodium (anhydrous
Na2HPO47.36g, citric acid 5.16g, distilled water are settled to 1L), the terminate liquid uses 2mol/L aqueous sulfuric acids.
As a kind of preferable technical scheme of the present invention, the titer in the detection reagent detection uses 99.7% pair of sulphur
Phosphorus, 99.1% parathion-methyl and 99.0% fenifrothion.
As a kind of preferable technical scheme of the present invention, the use in the detection reagent detection is through horseradish peroxidase
The sheep anti-mouse igg antibody and horseradish peroxidase chemical luminous substrate ultra stable A liquid and B liquid of mark.
It is immune with haptens, competition half in the detection reagent detection as a kind of preferable technical scheme of the present invention
Antigen, enzyme mark haptens, monoclonal antibody are made by oneself by laboratory.
As a kind of preferable technical scheme of the present invention, being carried out using cell line 7B2 in the detection reagent detection is small
The preparation purifying of mouse ascites fluid, obtains wide general monoclonal antibody.
Compared with prior art, the beneficial effects of the invention are as follows：
By the present invention in that with configuring complete kit, while ELISA Plate and drop rifle are configured with, and passed through by dripping rifle to molten
The absorption of solution in liquid bottle, and by the way that by the test paper placement of standard testing strips room, in the reaction chamber, the solution for dripping rifle instills from drip hole,
So as to realize the experimental reaction of the primary of kit, ensure accuracy when solution uses, ELISA Plate and drop rifle are integrated into examination
In agent box, increase its universality, avoid the infection using other drop slings, influence testing result；
, can be in optimal antigen, anti-in the detection using the wide general monoclonal antibody easily prepared, and by specificity identification
The lower accurately detection light absorption value of bulk concentration effect, so as to reach the cross reacting rate under ideal state, the secretion of 7B2 cell lines
Wide general monoclonal antibody can identify sulphur phosphorus, parathion-methyl and fenifrothion simultaneously, so as to organophosphor detection in have
Higher sensitivity, specific a variety of organophosphors detect, and improve the detection speed of organophosphor in food.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
In figure：1- box bodys；2- ELISA Plates room；3- standard testing strips room；4- is coated with solution；5- routine liquid；6- confining liquids；7- is washed
Liquid；8- competes buffer solution；9- ELIAS secondary antibody buffer solutions；10- nitrite ions；11- terminate liquids；12- reative cells；13- imbibition rifles room；
14- drip hole；15- orifice plates.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
The explanation of following embodiment is refer to the attached drawing, can be to the specific embodiment implemented to the example present invention.
The direction and position term that the present invention is previously mentioned, for example, " on ", " in ", " under ", "front", "rear", "left", "right", " interior ", "
Outside ", " side " etc., only it is direction and position with reference to annexed drawings.Therefore, the direction and position term used is to illustrate
And understand the present invention, and it is not used to the limitation present invention.
Embodiment：
As shown in figure 1, be used to detect the food inspection kit of organophosphor the invention provides a kind of, experimental article include box
Body 1, the leading flank bottom of box body 1 are provided with ELISA Plate room 2, and the side of the box body 1 is provided with drop rifle room 13, the box body
Standard testing strips room 3 and reative cell 12 are set side by side with inside 1, the surface of reative cell 12 is provided with drip hole 14, the standard examination
Paper room 3 and the top of reative cell 12 set abacus 15, and the putting hole of solution reagent bottle is provided with the orifice plate 15, described to put
Putting hole includes coating buffer solution 4, conventional liquid 5, confining liquid 6, cleaning solution 7, competition buffer solution 8, ELIAS secondary antibody buffer solution 9, colour developing
Liquid 10, terminate liquid 11.
In food inspection is carried out, coating buffer solution 4 is 0.05mol/L, pH9.6 carbonate buffer solution, and conventional liquid 5 is adopted
With 0.01mol/L, pH7.4 phosphate buffer, competition buffer solution 8 uses 0.1mol/L, pH8.5 phosphate buffer,
ELIAS secondary antibody buffer solution 9 uses PBS of the mass fraction for 2% skimmed milk power.Confining liquid 6 and ELIAS secondary antibody buffer solution 9 are adopted
Solution is same, and cleaning solution 7 uses the PBS containing 0.05%Tween-20, and nitrite ion 10 uses adjacent benzene disodium (anhydrous
Na2HPO47.36g, citric acid 5.16g, distilled water are settled to 1L), terminate liquid 11 uses 2mol/L aqueous sulfuric acids.
It is immune with haptens, competition haptens, enzyme mark in the preparation material detected, the detection reagent detection
Haptens, monoclonal antibody are made by oneself by laboratory, the titer in detection reagent detection using 99.7% parathion,
99.1% parathion-methyl and 99.0% fenifrothion, the sheep through horseradish peroxidase-labeled is used in the detection reagent detection
Dynamics and horseradish peroxidase chemical luminous substrate ultra stable A liquid and B liquid, adopted in the detection reagent detection
The preparation that mouse ascites are carried out with cell line 7B2 is purified, and obtains wide general monoclonal antibody.
In the present invention, specific operating method is：
Step 1: detection process, fresh fruit and vegetables are carried out to food：Weigh the sample that 5g is shredded in advance；
Step 2: adding 10mL acetonitrile mechanical shaking extraction 10min, 1g sodium chloride and 5g magnesium sulfate are added, vibration manually shakes up 3min,
5min is centrifuged under 4000r/min, takes 1mL supernatants to be dried up with nitrogen, adds the PB bufferings of the methanol containing volume fraction 10% in right amount
Liquid, vortex oscillation 1min, in 96 hole enzyme mark blackboards, add the parathion antigen diluted with coating buffer solution, 100 μ L/ holes, in 4
It is coated with DEG C overnight (about 12h), washing, adds the μ L/ holes of confining liquid 300,1h is incubated at 37 DEG C；
Step 3: washing, while add the pesticide standard solution of different gradient dilutions and each 50 μ of antibody-solutions of buffer solution dilution
L, mixed 1min is vibrated, 1h is incubated at 37 DEG C；
Step 4: washing again, chemical luminous substrate A liquid and B liquid (volume ratio 1 are taken respectively:1) it is, quiet under lucifuge, room temperature condition
20min is put, is mixed, is added by the amount in 150 μ L/ holes, after adding substrate, with lighting at chemiluminescence plate reader measure 425nm
Value.
In summary, the main characteristic of the invention lies in that：
By the present invention in that with configuring complete kit, while ELISA Plate and drop rifle are configured with, and passed through by dripping rifle to molten
The absorption of solution in liquid bottle, and by the way that by the test paper placement of standard testing strips room, in the reaction chamber, the solution for dripping rifle instills from drip hole,
So as to realize the experimental reaction of the primary of kit, ensure accuracy when solution uses, ELISA Plate and drop rifle are integrated into examination
In agent box, increase its universality, avoid the infection using other drop slings, influence testing result；
, can be in optimal antigen, anti-in the detection using the wide general monoclonal antibody easily prepared, and by specificity identification
The lower accurately detection light absorption value of bulk concentration effect, so as to reach the cross reacting rate under ideal state, the secretion of 7B2 cell lines
Wide general monoclonal antibody can identify sulphur phosphorus, parathion-methyl and fenifrothion simultaneously, so as to organophosphor detection in have
Higher sensitivity, specific a variety of organophosphors detect, and improve the detection speed of organophosphor in food.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the involved claim of limitation.
Claims (7)
- A kind of 1. food inspection kit for being used to detect organophosphor, it is characterised in that：Including box body（1）, the box body（1）Before Side bottom is provided with ELISA Plate room（2）, the box body（1）Side be provided with drop rifle room（13）, the box body（1）Inside is simultaneously Row are provided with standard testing strips room（3）And reative cell（12）, the reative cell（12）Surface is provided with drip hole（14）, the standard examination Paper room（3）And reative cell（12）Top set abacus（15）, the orifice plate（15）On be provided with the placement of solution reagent bottle Hole, the putting hole include coating buffer solution（4）, conventional liquid（5）, confining liquid（6）, cleaning solution（7）, competition buffer solution（8）, enzyme Mark secondary antibody buffer solution（9）, nitrite ion（10）, terminate liquid（11）.
- A kind of 2. food inspection kit for being used to detect organophosphor according to claim 1, it is characterised in that：The bag By buffer solution（4）For 0.05mol/L, pH9.6 carbonate buffer solution, the conventional liquid（5）Using 0.01mol/L, pH7.4's Phosphate buffer, the competition buffer solution（8）Using 0.1mol/L, pH8.5 phosphate buffer, the ELIAS secondary antibody delays Fliud flushing（9）Use PBS of the mass fraction for 2% skimmed milk power.
- A kind of 3. food inspection kit for being used to detect organophosphor according to claim 1, it is characterised in that：The envelope Close liquid（6）With ELIAS secondary antibody buffer solution（9）The solution of use is same, the cleaning solution（7）Using containing 0.05%Tween-20's PBS, the nitrite ion（10）Using adjacent benzene disodium (anhydrous Na 2HPO47.36g, citric acid 5.16g, distilled water constant volume To 1L), the terminate liquid（11）Using 2mol/L aqueous sulfuric acids.
- A kind of 4. food inspection kit for being used to detect organophosphor according to claim 1, it is characterised in that：The inspection Titer in test agent detection uses 99.7% parathion, 99.1% parathion-methyl and 99.0% fenifrothion.
- A kind of 5. food inspection kit for being used to detect organophosphor according to claim 1, it is characterised in that：The inspection Using the sheep anti-mouse igg antibody through horseradish peroxidase-labeled and horseradish peroxidase chemiluminescence bottom in test agent detection Thing ultra stable A liquid and B liquid.
- A kind of 6. food inspection kit for being used to detect organophosphor according to claim 1, it is characterised in that：The inspection Immune in test agent detection is made by oneself with haptens, competition haptens, enzyme mark haptens, monoclonal antibody by laboratory.
- A kind of 7. food inspection kit for being used to detect organophosphor according to claim 1, it is characterised in that：The inspection The preparation purifying of mouse ascites is carried out in test agent detection using cell line 7B2, obtains wide general monoclonal antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711324557.0A CN107807237A (en) | 2017-12-13 | 2017-12-13 | A kind of food inspection kit for being used to detect organophosphor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711324557.0A CN107807237A (en) | 2017-12-13 | 2017-12-13 | A kind of food inspection kit for being used to detect organophosphor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107807237A true CN107807237A (en) | 2018-03-16 |
Family
ID=61579901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711324557.0A Pending CN107807237A (en) | 2017-12-13 | 2017-12-13 | A kind of food inspection kit for being used to detect organophosphor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107807237A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1677108A (en) * | 2004-04-02 | 2005-10-05 | 韩丽君 | Enzyme-linked immunosorbentassay reagent kit for analyzing snout-killing sulfur-phosphous residual |
| CN101477123A (en) * | 2009-01-13 | 2009-07-08 | 华南农业大学 | ELISA reagent kit for detecting two oxyethyl group thiophosphate organophosphorus pesticide |
| CN203083922U (en) * | 2013-02-06 | 2013-07-24 | 江苏创生生物技术有限公司 | High-flux organophosphorus pesticide testing box |
| CN203981679U (en) * | 2014-06-23 | 2014-12-03 | 河南知微生物工程有限公司 | Malachite green vestigial detects ELISA kit |
| CN204039403U (en) * | 2014-04-17 | 2014-12-24 | 中国人民解放军军事医学科学院军事兽医研究所 | Portable animal pathogenic micro-organism rapid detection box |
| CN105044325A (en) * | 2015-06-18 | 2015-11-11 | 北京勤邦生物技术有限公司 | Enzyme-linked immunosorbent assay kit for detecting triazophos and application of enzyme-linked immunosorbent assay kit |
| CN205749526U (en) * | 2016-05-13 | 2016-11-30 | 中博瑞康（北京）生物技术有限公司 | A kind of ELISA detection kit |
| CN206648841U (en) * | 2016-11-03 | 2017-11-17 | 辽宁省环境监测实验中心 | The organic project sampling fixative case of water quality |
| CN207992230U (en) * | 2017-12-13 | 2018-10-19 | 百奥森（江苏）食品安全科技有限公司 | A kind of food inspection kit for detecting organophosphor |
-
2017
- 2017-12-13 CN CN201711324557.0A patent/CN107807237A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1677108A (en) * | 2004-04-02 | 2005-10-05 | 韩丽君 | Enzyme-linked immunosorbentassay reagent kit for analyzing snout-killing sulfur-phosphous residual |
| CN101477123A (en) * | 2009-01-13 | 2009-07-08 | 华南农业大学 | ELISA reagent kit for detecting two oxyethyl group thiophosphate organophosphorus pesticide |
| CN203083922U (en) * | 2013-02-06 | 2013-07-24 | 江苏创生生物技术有限公司 | High-flux organophosphorus pesticide testing box |
| CN204039403U (en) * | 2014-04-17 | 2014-12-24 | 中国人民解放军军事医学科学院军事兽医研究所 | Portable animal pathogenic micro-organism rapid detection box |
| CN203981679U (en) * | 2014-06-23 | 2014-12-03 | 河南知微生物工程有限公司 | Malachite green vestigial detects ELISA kit |
| CN105044325A (en) * | 2015-06-18 | 2015-11-11 | 北京勤邦生物技术有限公司 | Enzyme-linked immunosorbent assay kit for detecting triazophos and application of enzyme-linked immunosorbent assay kit |
| CN205749526U (en) * | 2016-05-13 | 2016-11-30 | 中博瑞康（北京）生物技术有限公司 | A kind of ELISA detection kit |
| CN206648841U (en) * | 2016-11-03 | 2017-11-17 | 辽宁省环境监测实验中心 | The organic project sampling fixative case of water quality |
| CN207992230U (en) * | 2017-12-13 | 2018-10-19 | 百奥森（江苏）食品安全科技有限公司 | A kind of food inspection kit for detecting organophosphor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107817354A (en) | A kind of chemiluminescence detection kit of interleukin 6 and preparation method thereof | |
| CN106093381B (en) | Rod method toxin rod method phenol monomethyl ether colloidal gold immuno-chromatography test paper strip | |
| WO2008014709A1 (en) | Analysis device for biologicla sample | |
| CN104569404A (en) | Method for detecting olaquindox by directly-competing TRFIA (Time Resolved Fluorescence Immunoassay) | |
| CN107727864A (en) | The protein chip of abnormal decarboxyprothrombin, kit and preparation method thereof in a kind of detection serum | |
| Aliotta et al. | Thrombocytopathies: not just aggregation defects—the clinical relevance of procoagulant platelets | |
| CN104849251B (en) | A kind of the time-resolved fluoroimmunoassay method and kit of quick detection gutter oil | |
| CN104109206A (en) | Monoclonal antibodies against duck Tembusu virus, antigen detection kit and application | |
| CN101307303B (en) | Kit for detecting clenobuterol hydrochloride residue and method for preparing same | |
| CN107966561A (en) | A kind of test strips and kit for detecting HIV antibody | |
| CN102226808A (en) | Trypsinogen-2 chemiluminescent immunoassay kit and preparation method thereof | |
| CN103288872A (en) | Methyl parathion hapten, and preparation method and application thereof | |
| CN102768278A (en) | Monoclonal antibody, enzyme-linked immunosorbent assay method and kit for detecting beta-receptor stimulant | |
| CN207992230U (en) | A kind of food inspection kit for detecting organophosphor | |
| CN102766212A (en) | Monoclonal antibody, enzyme-linked immunosorbent assay method and kit for detecting residues of fluoroquinolones | |
| CN102608316A (en) | Kit or test strip for detecting quinoxaline compound | |
| CN103018460A (en) | ELISA (enzyme-linked immuno sorbent assay) methods for detecting dibutyl phthalate content | |
| CN109180519B (en) | Olaquindox metabolite antigen, antibody, enzyme-linked immunosorbent assay kit and detection method | |
| CN107807237A (en) | A kind of food inspection kit for being used to detect organophosphor | |
| CN102590519A (en) | Kit or test strip for detecting aflatoxin | |
| CN103288661A (en) | Preparation method and application of malachite green hapten | |
| CN106771134B (en) | A kind of carp herpesvirusⅡtype sandwich ELISA detection kit and detection method | |
| CN207923895U (en) | The multi-test-line immunochromatographitest test strip of semidefinite quantization detection Hostathion pesticide | |
| CN105136779A (en) | Chemiluminescence immune kit for detection of aflatoxin M1 and application thereof | |
| CN107305212B (en) | A kind of immunological detection method and kit of organophosphate and carbamate pesticide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180316 |