CN217809446U - High-throughput full-automatic extractor for exosome - Google Patents

Abstract

The utility model relates to a full-automatic appearance that draws of exosome high flux relates to medical instrument's technical field, it includes liquid level automatic detection mechanism, multi-axis manipulator mechanism, disinfection filtering mechanism and the consumptive material that is used for the sample in-process to make used experiment consumptive material to collect and collects the mechanism, wherein liquid level automatic detection mechanism can effectual detection sample liquid level, accurate calculation sample draws and the addition volume, multi-axis manipulator mechanism then realizes that the sample draws and the removal of consumptive material is automatic, disinfection filtering mechanism has then guaranteed the health of drawing the in-process, cross contamination has effectively been prevented. This application realizes the full-automatic extraction of exosome, places sample and various experiment consumptive materials after, starts the instrument, and other process actions all adopt full-automatic mechanization, do not have artificial participation, and many samples once extract, have both improved efficiency, have guaranteed the health of extraction process again, guarantee the uniformity that the sample extracted the result when having reduced the cross infection risk.

Description

High-throughput full-automatic extractor for exosome

Technical Field

The application relates to the technical field of medical equipment, in particular to a high-flux full-automatic extractor for exosomes.

Background

Exosomes are extracellular vesicles produced by cells and contain important intercellular communication media, such as nucleic acids, proteins, and the like. The role of exosomes in immune responses has been documented in a large body of literature and clinical data. In addition, in the research of exosome and disease treatment, cardiovascular diseases, neurodegenerative diseases, early cancer diagnosis, new coronary pneumonia and other respiratory diseases are closely related to exosome. Meanwhile, the exosome can be applied to disease diagnosis and drug therapy carriers.

It can be seen that the exosome has a very deep application prospect for clinical medicine and advanced medical research. As exosome extraction needs a relatively professional biological medical experiment foundation, manual extraction is not beneficial to large-scale popularization, and a mature stable extraction kit does not exist at present. In view of the important effects of exosome in medical diagnosis and drug therapy, a mechanism capable of efficiently extracting exosome is urgently needed for eliminating manual interference of manual extraction, so that the extraction result is ensured to be real and reliable, and cross contamination is avoided.

In view of the above-mentioned related art, the inventors consider that there is a drawback that the extraction of exosomes is not accurate and efficient enough.

Disclosure of Invention

The utility model provides a full-automatic appearance that draws of exosome is drawed to accurate high efficiency aims at improving present traditional exosome and draws method step numerous drawback, promotes the true reliability of drawing the result.

The application provides a full-automatic appearance that draws of exosome high flux includes following technical scheme, the full-automatic appearance that draws of exosome high flux, including the base, be provided with on the base:

the device comprises a placing platform, a positioning device and a control device, wherein the placing platform is used for placing experimental equipment, an experimental sample and an experimental reagent;

the liquid level detection mechanism detects the liquid level of the experimental sample and records the position;

the multi-axis manipulator mechanism comprises a first support frame and a second support frame, one end of the second support frame is connected to the first support frame in a sliding mode, a sampling assembly is arranged on the second support frame in a sliding mode, and an included angle is formed between the motion track of the second support frame and the motion track of the sampling assembly;

the sampling assembly comprises a sixth driving part and a sampling part, and the sampling part is driven by the sixth driving part to reciprocate up and down;

the disinfection and filtration mechanism comprises a material shifting assembly, a pressurizing and pressure maintaining assembly and a disinfection assembly, the material shifting assembly is connected to the base in a sliding mode, the disinfection assembly is arranged on the material shifting assembly, and the pressurizing and pressure maintaining assembly is arranged on a sliding path of the material shifting assembly on the base; the material shifting assembly moves the experimental sample to a corresponding position, the pressurizing and pressure maintaining assembly pressurizes and filters the experimental sample, and the sterilizing assembly sterilizes the pressurizing and pressure maintaining assembly and other mechanical structures contacting the sample;

and the consumable collecting mechanism is used for collecting the used experimental consumables in the sampling process.

The traditional exosome extraction steps are various and often comprise sampling, pipetting, filtering, disinfection and the like; the sampling and pipetting process is very fine, the experiment operation requirement is high, the precision is often insufficient when the manual extraction is carried out, and time and labor are consumed; generally, conventional experimental equipment includes reagent tubes, reagent racks, tip heads, etc., and a placing platform is provided for placing the above experimental equipment.

Through adopting above-mentioned technical scheme, liquid level automatic checkout mechanism can detect the liquid level of experiment sample to its position of record, then cooperate multi-axis manipulator mechanism in order to realize the accurate of experiment sample volume and draw.

The multi-shaft mechanical arm mechanism is connected with the first support frame in a sliding mode through the second support frame, the sampling assembly is connected with the second support frame in a sliding mode to achieve moving sampling in a plane, the sampling assembly moves up and down on the first driving piece to achieve three-dimensional sampling, automatic sampling is achieved, operation is stable, and precision is high; the test tube that the mechanism was retrieved to the consumptive material used when carrying out the automatic extraction separation to the exosome, experiment consumptive materials such as filter membrane pipe in time retrieve, the follow-up reuse of being convenient for or concentrate unified processing. The mechanism realizes the full-automatic extraction process of exosome, effectively avoids pollution and errors caused by manual operation, and ensures the real reliability of the extraction result.

Optionally, the liquid level automatic detection mechanism comprises a liquid level detection up-down moving assembly, and the liquid level detection up-down moving assembly comprises a moving plate, a second driving piece and a detector mounting plate; the second driving piece comprises a first motor and a first screw rod, the first screw rod is perpendicular to the moving plate and is connected with the first screw rod, a first lifting block is arranged on the first screw rod, and the detector mounting plate is connected with the first lifting block; the detector is arranged on the detector mounting plate and used for detecting the liquid level position of the experiment sample.

Through adopting above-mentioned technical scheme, thereby first motor drive first lead screw rotates and drives first elevator to reciprocate to the detector mounting panel that makes and be connected with first elevator reciprocates, thereby drives the detector and reciprocates and detect the liquid level height of experiment sample.

Optionally, the liquid level automatic detection mechanism further comprises a liquid level detection horizontal movement assembly, the liquid level detection horizontal movement assembly comprises a first mounting frame, and a first driving piece and a first transmission piece which are arranged on the first mounting frame, and the first driving piece is connected with the first transmission piece;

the moving plate is connected with the first mounting rack in a sliding manner and is fixedly connected with the first conveying piece; the moving plate is driven by the first driving piece to horizontally move along with the transmission of the first driving piece.

Through adopting above-mentioned technical scheme, set up the liquid level and detect the liquid level that the horizontal migration subassembly realized different experimental samples on the horizontal direction and detect.

Optionally, the automatic liquid level detection mechanism further comprises a sample grabbing and rotating assembly, wherein the sample grabbing and rotating assembly comprises a third driving piece, a third transmission piece, a rotating shaft and a grabbing piece; one end of the third transmission piece is connected with the third driving piece, and the other end of the third transmission piece is connected with the rotating shaft.

The grabbing piece comprises a clamping jaw and a first cylinder, the rotating shaft is connected with the first cylinder, and the clamping jaw is symmetrically arranged on two sides of the first cylinder.

By adopting the technical scheme, the third driving part drives the third transmission part to drive the rotating shaft to rotate, the rotating shaft rotates to drive the grabbing part connected with the rotating shaft to rotate, and the clamping jaws are driven by the first air cylinder to mutually approach or separate, so that the clamping and positioning of the reagent tube for containing the experimental sample are realized, and the detector arranged on the detector mounting plate can conveniently and accurately measure the liquid level of the experimental sample; the clamping jaw is more adaptive to the outer wall of the reagent tube, and the reagent tube is convenient to stably clamp.

Optionally, the side surfaces of the clamping jaws which are close to each other are arranged in an arc shape.

Optionally, the sides of the clamping jaws close to each other can also be provided with wave shapes, horseshoe shapes, conical shapes and the like.

Through adopting above-mentioned technical scheme, the clamping jaw is more the adaptation with the outer wall of reagent pipe, plays skid-proof effect, and convenient stable clamp is got.

Optionally, a third support frame is further connected to the second support frame in a sliding manner, a clamping assembly is arranged on the third support frame, the clamping assembly comprises a clamping piece and a fourth driving piece, and the fourth driving piece drives the clamping piece to clamp the experimental device.

Optionally, the material shifting assembly includes an operating platform, the operating platform is connected to the base in a sliding manner, and a plurality of positioning holes are formed in the operating platform.

Through adopting above-mentioned technical scheme, the operation platform of connection on the base that slides can realize the removal to the experiment sample position, and the experimental device can be fixed to the locating hole in addition, and the experimental device here specifically is support and filter membrane pipe assembly.

Optionally, the pressurizing and pressure maintaining assembly is arranged above one end of the operation platform, the pressurizing and pressure maintaining assembly includes a support member, a cover plate and a seventh driving member, and the seventh driving member includes an air compressor; the air compressor is arranged on the supporting piece, the cover plate is connected with the second cylinder air compressor, and the cover plate is driven by the air compressor and the air compressor to move up and down in a reciprocating mode.

Optionally, the cover plate is provided with a plurality of pressurizing holes, and the pressurizing holes are communicated with the pressure pump through air pipes.

By adopting the technical scheme, when a sample placed in the filter membrane tube assembly moves to the position right below the pressurizing and pressure maintaining assembly at the material displacement assembly, the air compressor drives the cover plate to move downwards to be matched with the filter membrane tube filled with the sample, and the pressure pump generates pressure to apply the pressure to the sample in the filter membrane tube through the pressurizing hole, so that the filtering operation is completed.

Optionally, the sterilizing assembly comprises a sterilizing reagent bottle and an extrusion piece, and the sterilizing reagent bottle contains a sterilizing reagent; the extrusion part comprises a third air cylinder and an extrusion block; one end of the extrusion block is connected with the third cylinder, and the other end of the extrusion block is connected with the disinfection reagent bottle.

Through adopting above-mentioned technical scheme, the disinfection reagent (if etc.) that holds in, under the drive of third cylinder, extrusion piece extrusion disinfection reagent bottle, disinfection reagent bottle takes place deformation and extrudes the disinfection reagent of inside and spray to disinfect.

Optionally, the consumable collecting mechanism comprises a transmission assembly, a recycling tank and a storage cavity;

the recycling tank is arranged between the placing platform and the operating platform;

the transmission assembly comprises a fifth driving piece and a transmission belt, the transmission belt is arranged at the bottom of the recycling groove, and the transmission belt is communicated with the recycling groove and the storage cavity.

Through adopting above-mentioned technical scheme, used consumptive material when taking a sample at every turn needs in time to be changed, gets into from the accumulator, then arrives under transmission device's transportation and deposits the chamber and retrieve and deposit.

Optionally, the aperture of the notch of the recovery tank is gradually reduced from top to bottom.

Through adopting above-mentioned technical scheme, the accumulator plays the guide effect, and the place outside the accumulator can not drop to dumped experiment consumptive material.

To sum up, the exosome high-throughput full-automatic extractor provided by the application has the following beneficial effects: can be to the good sample liquid of layering, through special reagent, filter membrane and clean container support, through adding reagent, atmospheric pressure pressurization pressurize, filtration, sample automatic transfer to and steps such as disinfection that prevent cross infection realize exosome full automatization and draw, can effectual improvement exosome efficiency of drawing and the reliability of finally drawing the structure.

Drawings

Fig. 1 is a schematic diagram of an internal structure of an exosome high-throughput fully-automatic extractor disclosed in an embodiment of the present application;

fig. 2 is a schematic structural diagram of a placement platform, experimental equipment and the like of the exosome high-throughput full-automatic extractor disclosed in the embodiment of the present application;

fig. 3 is a schematic structural diagram of a first view angle of an automatic liquid level detection mechanism of an exosome high-throughput full-automatic extractor disclosed in the embodiment of the present application;

fig. 4 is a schematic structural diagram of a second view angle of an automatic liquid level detection mechanism of an exosome high-throughput full-automatic extractor disclosed in the embodiment of the present application;

FIG. 5 is a schematic diagram of a partial structure of an exosome high-throughput fully-automatic extractor disclosed in the embodiment of the present application;

FIG. 6 is an enlarged view at A in FIG. 5;

FIG. 7 is a schematic structural diagram of a sampling assembly and a TIP head mounting and dismounting assembly of the exosome high-throughput fully-automatic extractor disclosed in the embodiment of the present application;

FIG. 8 is an enlarged view at B in FIG. 5;

FIG. 9 is a schematic structural diagram of a disinfection and filtration mechanism of an exosome high-throughput fully-automatic extractor disclosed in the embodiment of the present application;

FIG. 10 is an enlarged view at C of FIG. 9;

fig. 11 is a schematic structural diagram of a consumable collecting mechanism of an exosome high-throughput full-automatic extractor disclosed in the embodiment of the present application.

Description of reference numerals:

1. a base; 10. a frame; 100. a fixing plate; 101. a partition plate; 102. a support plate; 1021. a limiting plate;

2. placing a platform; 20. mounting holes; 21. test tube rack, 211, test tube; 22. a collection tube holder; 221. a collection pipe; 23. TIP head holder; 231. a TIP header; 24. a filter membrane tube assembly; 241. a membrane filtering pipe; 242. a filter membrane tube support;

3. a liquid level automatic detection mechanism;

301. a first mounting bracket; 3010. accommodating grooves; 302. a second mounting bracket; 303. a third mounting bracket;

31. a liquid level detection horizontal movement assembly; 311. a substrate; 3111. a slide rail; 3112. a first origin sensor; 312. a first driving member; 313. a first transmission member; 3131. a driving wheel; 3132. a driven wheel; 3133. a conveyor belt;

32. a liquid level detection up-and-down moving assembly; 321. moving the plate; 322. a second driving member; 3221. a first motor; 3222. a first lead screw; 323. a detector mounting plate; 324. a lifting block; 325. a slider;

33. a sample capture rotation assembly; 331. a third driving member; 332. a third transmission member; 333. a rotating shaft; 334. grasping the part; 3341. a clamping jaw; 3342. a first cylinder; 335. a second origin sensor;

4. a multi-axis manipulator mechanism;

401. a first support frame; 402. a second support frame; 403. a third support frame; 4031. mounting a plate; 4032. a connecting frame;

41. a gripping assembly; 411. a clamping member; 412. a fourth drive member; 413. a second motor; 414. a second lead screw;

42. a sampling assembly; 421. sampling; 422. An air pump;

43. assembling and disassembling a TIP head; 431. a limiting sliding sleeve; 432. assembling and disassembling the cylinder;

5. a sterilizing and filtering mechanism;

51. a material displacement assembly; 511. an operating platform; 5111. a first operating region; 5112. a second operation area; 512. a third motor;

52. a pressurizing and pressure maintaining component; 521. a support member; 522. a cover plate; 5220. a pressurizing hole; 523. a second cylinder; 524. a sealing gasket;

53. a sterilizing assembly; 531. a sterilizing nozzle; 532. sterilizing the reagent bottle; 533. a guide seat; 534. extruding the block; 535. a third cylinder;

54. a reagent addition component; 541. a mounting base; 542. a silicone tube; 543. a peristaltic pump;

6. a consumable collection mechanism;

61. a recovery tank; 610. a guide port;

62. a transmission assembly; 621. a fifth driving member; 622. a conveyor belt;

63. a storage chamber;

64. a drawer; 641. a side plate; 642. a base plate; 643. and (4) a handle.

Detailed Description

The present application is described in further detail below with reference to fig. 1-11.

For the convenience of understanding the embodiments of the present application, the directions mentioned below are now explained, and the directions of X, Y and the Z axis are defined with reference to the drawings, and the terms such as up, down, left, right, and the like are used herein for better understanding of the present application and should not be construed as limiting the present application.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present application, "plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be interconnected within two elements or in a relationship where two elements interact with each other unless otherwise specifically limited. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

Example (b): referring to fig. 1 and 2, the exosome high-throughput full-automatic extractor comprises a base 1, wherein a placing platform 2, a liquid level automatic detection mechanism 3, a multi-axis manipulator mechanism 4, a disinfection filtering mechanism 5 and a consumable collecting mechanism 6 are arranged on the base 1. Place experimental sample, experimental reagent and experimental device on place platform 2, be provided with a plurality of mounting holes 20 on place platform 2 for above experimental device can stably fix on relevant position. After starting the exosome high-flux full-automatic extraction instrument, the liquid level automatic detection mechanism 3 can automatically move to the position where the sample is located and detect the liquid level height of the sample, and record the liquid level position, then the multi-axis manipulator mechanism 4 can clamp the support on the placing platform 2 and the filter membrane tube 241 and move to the operation platform 511 of the disinfection and filtration mechanism 5, then a series of operations such as sample transfer, filtration and pressure maintaining are carried out, and finally the target object is extracted. In each sampling, the TIP head 231 in contact with the sample or reagent needs to be sterilized and automatically replaced, the used filter membrane tube 241 is discarded, and the waste is recycled and transported out through the consumable collecting mechanism 6; the obtained target is transferred to the collection pipe 221 by the multi-axis robot mechanism 4, and the used stent is restored and restored by the multi-axis robot mechanism 4. The whole operation flow is full-automatic, manual intervention is not needed, the extraction efficiency is high, and cross contamination can be effectively reduced, so that the extraction structure is guaranteed to be real and reliable.

Referring to fig. 2, a plurality of experimental devices, specifically, reagents required for extracting exosomes, are placed on the placement platform 2, and the experimental devices include TIP heads 231 and TIP head holders 23, test tubes 211 and test tube holders 21, collection tube holders 22 and collection tubes 221, filter membrane tube assemblies 24, and the like. The filter membrane tube assembly 24 includes a filter membrane tube 241 and a filter membrane tube holder 242, and is mainly used for filtering an exosome sample. In order to strengthen the stability of the experimental reagent and the experimental equipment, a plurality of corresponding mounting holes 20 are formed in the placing platform 2, and the mounting holes 20 are circular and square.

Referring to fig. 3 and 4, the liquid level automatic detection mechanism 3 includes a liquid level detection horizontal movement module 31, a liquid level detection up-down movement module 32, a sample gripping rotation module 33, a first mounting bracket 301 on which the liquid level detection horizontal movement module 31 is mounted, a second mounting bracket 302 on which the liquid level detection up-down movement module 32 is mounted, and a third mounting bracket 303 on which the sample gripping rotation module 33 is mounted.

The liquid level detection horizontal moving component 31 drives the liquid level detection up-down moving component 32 and the sample grabbing rotating component 33 to move in the Y-axis direction, and the moving direction is consistent with the arrangement direction of the experimental sample on the placing platform 2; and detecting the experimental sample.

The liquid level detects and reciprocates subassembly 32 and liquid level detection horizontal component and slides and is connected, realizes detecting the liquid level of the different experimental samples on the place the platform 2. Specifically, the liquid level detection horizontal movement assembly 31 includes a substrate 311, a first driving member 312, and a first driving member 313; two first mounting brackets 301 are connected to base plate 311, are provided with holding tank 3010 on the first mounting bracket 301, and first driving piece 312 sets up in holding tank 3010, improves stability.

Specifically, the first driving member 312 is a motor, and the first driving member 313 includes a driving wheel 3131, a driven wheel 3132, and a transmission belt 3133 connecting the driving wheel 3131 and the driven wheel 3132. The belt 3133 is fixedly connected to the liquid level detection up-and-down moving assembly 32, and in this embodiment, a fixing block is fixed to the belt 3133, so that the liquid level detection up-and-down moving assembly 32 can move smoothly, and the accuracy of liquid level detection can be maintained.

With continued reference to fig. 3 and 4, the liquid level detection up-down moving unit 32 includes a moving plate 321, a second driving member 322, a detector mounting plate 323, and an elevating block 324 connecting the detector mounting plate 323 and the second driving member 322, and a detector (not shown) is fixedly mounted on the detector mounting plate 323. The second mounting block 302 is disposed on the moving plate 321, the second driving member 322 is disposed on the second mounting block 302, and the detector mounting plate 323 moves in the Z-axis direction by the driving of the second driving member 322. Specifically, the second driving member 322 includes a first motor 3221 and a first lead screw 3222, the lifting block 324 is connected to the lead screw, and the first motor 3221 drives the first lead screw 3222 to rotate, so as to drive the detector mounting plate 323 on the lifting block 324 to move up and down, thereby driving the detector to move. The detector 323 in the present embodiment is a laser sensor. Because the liquid levels of the experimental samples are layered, the color difference between different liquid level layers is obvious; when the liquid level of different colors passes through the laser sensor, the laser sensor can be triggered to give different signals, the position of the motor is transmitted to the control system, and the position coordinates are converted into position coordinates through calculation and conversion and are recorded and stored.

The first driving member 313 is connected to the moving plate 321, and the liquid level detection up-down moving assembly 32 moves in the Y-axis direction with the driving member under the driving of the first driving member 312.

Further, in order to reduce the resistance of the conveyor 3133 to the liquid level detection up-down moving unit 32, a slide rail 3111 is provided on the base plate 311, and a slider 325 is provided between the moving plate 321 and the base plate 311. The moving plate 321 is slidably connected to the base plate 311 via the slider 325, and then horizontally moves under the driving of the conveyor 3133.

Further, a first origin sensor 3112 is disposed on the substrate 311 to position and limit the moving plate 321, so as to achieve accurate detection.

A third mount 303 is provided on the second mount 302, and a sample grip rotation assembly 33 is provided on the third mount 303.

The sample grip rotation assembly 33 comprises a third drive member 331, a third transmission member 332, a rotation shaft 333 and a grip member 334. The third driving member 331 is disposed on the third mounting frame 303, the rotating shaft 333 passes through the third mounting frame 303 and is connected to the third transmission member 332, and is driven by the third driving member 331 to rotate, and the lower end of the rotating shaft 333 is provided with a gripping member 334. The gripping member 334 includes a clamping jaw 3341 and a first cylinder 3342, the clamping jaw 3341 is symmetrically disposed on two sides of the first cylinder 3342, and the clamping jaw 3341 grips the reagent tube of the experimental sample under the driving of the first cylinder 3342.

Further, in order to fit the arc of the wall of the test tube 211 of the reagent tube more, and to achieve stable clamping, the surfaces of the clamping jaws 3341 that are close to each other are set to be arc-shaped.

Further, a second origin sensor 335 is disposed on the third mounting bracket 303, particularly, near a connection of the rotation shaft 333 and the third mounting bracket 303. Accurate positioning of the sample capture rotation assembly 33 is achieved.

In the embodiment of the present invention, the third driving element 331 is a motor, and the third transmission element 332 is a transmission belt 3133, a driving wheel 3131 and a driven wheel 3132, and the implementation principle and the connection relationship thereof are conventional technical means, which are not described herein again.

Therefore, before the liquid level height position is detected, the first air cylinder 3342 is required to drive the clamping jaw 3341 to clamp the test tube 211 filled with the experimental sample, a signal is generated when the laser sensor rotates to the position, when the laser sensor detects the liquid level of one experimental sample, the liquid level of the next experimental sample is detected, the third driving piece 331 stops working, and the first air cylinder 3342 drives the clamping jaw 3341 to loosen; the liquid level detection horizontal moving mechanism drives the sample rotary gripping mechanism to move, and the first air cylinder 3342 and the clamping jaw 3341 are not interfered with the test tube 211 when moving.

Referring to fig. 5, a frame 10 is disposed on the base 1 for mounting the multi-axis manipulator mechanism 4, the frame 10 includes three fixing plates 100 disposed on the base 1 in parallel and perpendicular to each other and partition plates 101 connecting the three fixing plates 100, specifically, the fixing plates 100 are L-shaped plates, and openings are further disposed on the fixing plates 100. The setting of the fixed plate 100 of L type can effectual improvement stability on base 1, secondly sets up the trompil, does benefit to the operating condition of heat dissipation and observation inside spare part.

Referring to fig. 6, the multi-axis robot mechanism 4 includes a first support 401, a second support 402, and a third support 403, the first support 401 is disposed on the top end of the fixing plate 100 (i.e., the end of the fixing plate 100 away from the base 1), one end of the first support 401 is slidably connected to the second support 402, and the third support 403 is slidably connected to the second support 402. The first support frame 401 is disposed in the X-axis direction, and the second support frame 402 is disposed in the Y-axis direction, which are perpendicular to each other. The second support frame 402 slides on the first support frame 401 in the X-axis direction, and the third support frame 403 slides on the second support frame 402 in the Y-axis direction.

The third support frame 403 comprises a mounting plate 4031 and a connecting frame 4032, one end of the mounting plate 4031 is connected with the second support frame 402 in a sliding manner, and the other end of the mounting plate 4032 is connected with the connecting frame 4032. The third support frame 403 is provided with a clamping assembly 41 and a sampling assembly 42, the clamping assembly 41 is arranged on the mounting plate 4031, the sampling assembly 42 is arranged on the connecting frame 4032, the clamping assembly 41 and the sampling assembly 42 are positioned on two sides of the second support frame 402, the space layout is reasonable, and the sampling and clamping steps are not affected.

The clamping assembly 41 includes a clamping member 411 and a fourth driving member 412, specifically, the fourth driving member 412 is a cylinder, and the clamping member 411 is two clamping blocks symmetrically disposed on two sides of the cylinder. The cylinder drives the clamping blocks to be close to or far away from each other, so that the experimental equipment can be clamped and released. In the embodiment of the application, the experimental equipment is mainly a filter membrane tube assembly.

In order to realize the movement of the clamping assembly 41 in the Z-axis direction, a second motor 413 and a second lead screw 414 are provided, one end of the second lead screw 414 is connected to the second motor 413, then the first lead screw 3222 is connected to the air cylinder, the second motor 413 drives the second lead screw 414 to rotate, and the rotation of the second lead screw 414 is converted into driving the air cylinder to move up or down.

Furthermore, the side surfaces of the two clamping blocks, which are close to each other, are provided with positioning blocks (not shown in the figure), so that the clamping blocks can be accurately positioned when clamping the experimental equipment, and the clamping movement process is kept stable.

The sampling assembly 42 includes a sixth driving member and a sampling member 421, the sixth driving member is connected to the sampling member 421 and enables the sampling member 421 to move in the Z-axis direction. Sampling piece 421 is the middle sampling column that is equipped with the gas pocket, and the sampling principle of this application is the negative pressure sample, specifically sets up to: one end of the sampling piece 421 is in interference fit with the TIP head 231 (the diameter of the sampling piece 421 is slightly larger than that of the TIP head 231, so that the TIP head 231 can be clamped on the sampling piece 421), and the other end of the sampling piece 421 is connected with the air pump 422, so that negative pressure is generated in the sampling column through air suction to suck a sample, and the sample is discharged through air exhaust. In the embodiment of the present application, the sixth driving component is specifically a screw motor, that is, the sampling component 421 is driven by the screw motor to realize the movement in the Z-axis direction.

Similarly, the movement of the sampling assembly 42 in the Z-axis is also required during the sampling process, and the specific arrangement and principle are the same as those of the gripping assembly 41, which will not be described in detail herein.

Referring to fig. 7, the TIP head 231, which has been in contact with the test reagent or the test sample, needs to be replaced every time a sample is taken, in order to realize automatic replacement. The sampling assembly 42 is provided with a TIP head assembling and disassembling assembly 43, the TIP head assembling and disassembling assembly 43 comprises a limiting sliding sleeve 431 and an assembling and disassembling cylinder 432, the limiting sliding sleeve 431 is sleeved on the sampling piece 421 in a sliding manner, and the limiting sliding sleeve 431 can be abutted with the upper end edge of the TIP head 231; the assembling and disassembling cylinder 432 is fixedly connected to the connecting frame 4032, and a piston rod of the assembling and disassembling cylinder 432 is connected with the discharging sliding sleeve 431. The working principle is as follows: after the piston rod of the mounting and dismounting cylinder 432 extends, the limiting sliding sleeve 431 can separate and withdraw the TIP head 231 from the sampling piece 421, so that the TIP head 231 can be dismounted.

Referring to fig. 1 and 6, when an exosome is extracted, a relevant reagent needs to be added, in order to realize the function of automatically adding the reagent, the reagent adding assembly 54 is provided, the reagent adding assembly 54 comprises an installation seat 541, a silicone tube 542 and a peristaltic pump 543, and the silicone tube 542 is arranged on the installation seat 541 in a penetrating manner; the peristaltic pump 543 is communicated with the silicone tube 542, and the peristaltic pump 543 is also directly communicated with a vessel loaded with a reagent solution, so as to realize the adding of a large amount of reagent solution.

Referring to fig. 5 and 8, the frame 10 further includes a support plate 102, and the support plate 102 is connected to the three fixing plates 100 to form a stable support structure for supporting the sterilizing filter mechanism 5. The sterilizing and filtering mechanism 5 includes a material displacement assembly 51, a pressurizing and pressure maintaining assembly 52 and a sterilizing assembly 53. The material shifting assembly 51 comprises an operating platform 511, the operating platform 511 is connected with the supporting plate 102 in a sliding manner, and a driving assembly is arranged on the operating platform 511 to drive the operating platform 511 to move on the base 1 along the X-axis direction. In the embodiment of the present application, the driving component is specifically a third motor 512; the operation platform 511 is divided into a first operation area 5111 and a second operation area 5112, and the sampling operation is completed in the first operation area 5111, and then the operation platform is transferred to the second operation area 5112 to add reagents, perform filtration and disinfection. The operation platform 511 is provided with a positioning hole (not shown) for stably fixing the filter membrane bracket and keeping the stability of the filter membrane bracket.

Further, to limit the transitional movement of the manipulation platform 511, a stopper plate 1021 is provided at one end of the support plate 102 close to the first manipulation zone 5111.

The sliding connection between the operation platform 511 and the support plate 102, and the moving principle of the operation platform 511 in the X-axis direction are the same as the liquid level detection horizontal moving assembly 31, and are not described herein again.

Referring to fig. 8 and 9, the pressurizing and pressure maintaining assembly 52 is disposed above one end of the operation platform 511, and specifically includes a support 521, a cover plate 522, and a seventh driving member including an air compressor and a second cylinder 523. The supporting member 521 is specifically an L-shaped supporting column, and two ends of the supporting column are respectively fixedly connected with the base 1 and the partition plate 101; the second cylinder 523 is arranged at the upper end of the supporting column, the air compressor is arranged behind the second cylinder 523 (not shown in the figure), and the cover plate 522 is arranged below the driving cylinder and driven by the second cylinder 523 to move up and down along the Z-axis direction. That is, the cover plate 522 is driven by the second cylinder 523 to perform downward pressing and lifting actions, the air compressor provides air pressure, and when the cover plate 522 is pressed downward and sealed with the filter membrane pipe support, the pressurizing and pressure maintaining functions are realized.

Referring to fig. 10, the cover plate 522 is provided with a pressurizing hole 5220 matched with the holder of the test tube 211, the pressurizing hole 5220 is connected to a pressure pump through an air tube, and the sample in the filter tube 241 is pressurized to pass through the filter tube 241, thereby realizing filtration.

Specifically, one side of the cover plate 522, which is far away from the driving cylinder, is provided with a sealing gasket 524, so as to reinforce the sealing performance between the cover plate 522 and the support of the filter membrane tube 241, thereby better realizing pressure application and pressure maintaining.

When the experimental sample needs to be filtered, the filter membrane tube 241 is clamped from the first operation area 5111 to the second operation area 5112 by the clamping assembly 41, and then moved to the position right below the cover plate 522 by the driving cylinder on the operation platform 511, and the driving cylinder for pressurizing and maintaining pressure drives the cover plate 522 to cover the filter membrane tube bracket for pressure filtration.

Referring to fig. 9 and 10, the sterilizing assembly 53 is disposed on the operation platform 511, and includes a sterilizing nozzle 531, a sterilizing reagent bottle 532, a guide holder 533 for fixing the sterilizing reagent bottle 532, a pressing block 534 for pressing the reagent bottle, and a third cylinder 535, and the sterilizing reagent bottle 532 contains a sterilizing reagent. The squeezing block 534 is driven by the third cylinder 535 to squeeze the sterilization reagent bottle 532, and the disinfection solution is sprayed out from the reagent bottle and sprayed out through the disinfection nozzle 531 to disinfect the cover plate 522.

Referring to fig. 11, the consumable collecting mechanism 6 includes a recovery tank 61, a transport assembly 62, and a storage chamber 63. The accumulator 61 sets up between place platform 2 and operation platform 511, and the bore of the notch of accumulator 61 down reduces gradually from last, forms a spigot 610, makes things convenient for the collection of consumptive material, makes the consumptive material steadily drop in accumulator 61. The transmission assembly 62 is embodied as a transmission belt assembly, and includes a fifth driving element 621 and a transmission belt 622, in this embodiment, the fifth driving element includes a motor and a transmission belt, and a driving wheel and a driven wheel, and the transmission belt 622 is driven by the fifth driving element to rotate, so as to realize a transmission function. The connection relationship and principle of the fifth driving member 621 are conventional technical means in the industry, and will not be described herein too much.

The conveying belt 622 is communicated with the recycling groove 61 and the storage cavity 63, the fifth driving piece 621 drives the conveying belt 622 to transmit, consumables falling into the recycling groove 61 are conveyed to the storage cavity 63 to be temporarily stored, and the consumables are conveniently taken out and discarded subsequently. To further facilitate the collection and storage of consumables, a drawer 64 is provided to cover the storage chamber 63. The drawer 64 includes three side panels 641 and a bottom panel 642. A sliding guide rail is arranged on one side of the side wall of the storage cavity 63, which is far away from the storage cavity 63, and a side plate 641 of the drawer 64 is connected with the sliding guide rail in a sliding manner, so that the drawer 64 can be opened and closed at any time. In order to facilitate opening and closing of the drawer 64, a handle 643 is provided on a side plate 641 of the drawer 64.

The beneficial effect of the setting position of the recycling tank 61 is that the multi-axis manipulator mechanism 4 reciprocates between the placing platform 2 and the operating platform 511 to sample and clamp consumables such as the test tube 211 and the filter membrane tube 241 bracket, and the consumables pass through the recycling tank 61 at every time, namely the TIP head 231 is used once and then can be discarded in the recycling tank 61 on the way, so that the moving path of the multi-axis manipulator mechanism 4 is saved, and the efficiency is improved.

The implementation principle of the embodiment of the application is as follows: after the exosome high-flux full-automatic extractor is started, the liquid level detection mechanism can detect the liquid level of the experimental sample on the placing platform 2 and record the position; then the clamping component 41 clamps and moves the filter membrane tube 241 and the support of the filter membrane tube 241, and clamps the filter membrane tube 241 from the placing platform 2 to the operating platform 511; the sampling component 42 performs sampling, and after each sampling, the used TIP head 231 is pushed out by the pushing component and falls into the consumable collecting mechanism 6; the reagent adding assembly 54 adds reagent into the filter membrane tube 241, moves to a corresponding position through the material shifting assembly 51 for pressurization, pressure maintaining and sterilization, and finally collects the reagent into the collecting tube 221 through the sampling assembly 42, thereby completing the whole extraction process. The whole process is full-automatic, the efficiency is high, the precision is high, the cross contamination is reduced, and the accuracy and the reliability of an exosome extraction result are effectively ensured.

The embodiments of the present invention are preferred embodiments of the present application, and the protection scope of the present application is not limited thereby, wherein like parts are denoted by like reference numerals. Therefore: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims (10)

1. Full-automatic appearance that draws of exosome high flux, its characterized in that, including base (1), be provided with on base (1):

the device comprises a placing platform (2), wherein the placing platform (2) is used for placing experimental equipment, experimental samples and experimental reagents;

the automatic liquid level detection mechanism (3) detects the liquid level of the experimental sample and records the position of the liquid level;

the multi-axis manipulator mechanism (4) comprises a first support frame (401) and a second support frame (402), one end of the second support frame (402) is connected to the first support frame (401) in a sliding mode, a sampling assembly (42) is arranged on the second support frame (402) in a sliding mode, and an included angle is formed between the movement track of the second support frame (402) and the movement track of the sampling assembly (42);

the sampling assembly (42) comprises a sixth driving part and a sampling part (421), and the sampling part (421) is driven by the sixth driving part to reciprocate up and down;

the disinfection and filtration mechanism (5) comprises a material displacement component (51), a pressurizing and pressure maintaining component (52) and a disinfection component (53), the material displacement component (51) is connected to the base (1) in a sliding manner, the disinfection component (53) is arranged on the material displacement component (51), and the pressurizing and pressure maintaining component (52) is arranged on a sliding path of the material displacement component (51); the material shifting assembly (51) moves the experimental sample to a corresponding position, the pressurizing and pressure maintaining assembly (52) performs pressurizing and filtering on the experimental sample, and the sterilizing assembly (53) sterilizes the pressurizing and pressure maintaining assembly (52);

and the consumable collecting mechanism (6) is used for collecting experimental consumables used in the sampling process.

2. The exosome high-throughput full-automatic extraction instrument according to claim 1, characterized in that the liquid level automatic detection mechanism (3) comprises a liquid level detection up-and-down moving assembly (32), and the liquid level detection up-and-down moving assembly (32) comprises a moving plate (321), a second driving piece (322) and a detector mounting plate (323); the second driving part (322) comprises a first motor (3221) and a first lead screw (3222), the first lead screw (3222) is perpendicular to the moving plate (321), the first motor (3221) is connected with the first lead screw (3222), a first lifting block (324) is arranged on the first lead screw (3222), and the detector mounting plate (323) is connected with the first lifting block (324); the detector mounting plate (323) is provided with a detector, and the detector is used for detecting the liquid level position of the experimental sample.

3. An exosome high-throughput full-automatic extraction instrument according to claim 2, characterized in that the liquid level automatic detection mechanism (3) further comprises a liquid level detection horizontal moving assembly (31), the liquid level detection horizontal moving assembly (31) comprises a first mounting frame (301), and a first driving member (312) and a first transmission member (313) which are arranged on the first mounting frame (301), and the first driving member (312) is connected with the first transmission member (313);

the moving plate (321) is connected with the first mounting rack (301) in a sliding mode and is fixedly connected with the first transmission piece; the moving plate (321) is driven by the first driving piece (312) to move horizontally along with the transmission of the first transmission piece (313).

4. An exosome high-throughput full-automatic extraction instrument according to claim 1, characterized in that the liquid level automatic detection mechanism (3) further comprises a sample-grasping rotary component (33), and the sample-grasping rotary component (33) comprises a third driving member (331), a third transmission member (332), a rotating shaft (333) and a grasping member (334); one end of the third transmission piece (332) is connected with the third driving piece (331), and the other end of the third transmission piece is connected with the rotating shaft (333);

the grabbing piece (334) comprises a clamping jaw (3341) and a first air cylinder (3342), the rotating shaft (333) is connected with the first air cylinder (3342), and the clamping jaw (3341) is symmetrically arranged on two sides of the first air cylinder (3342).

5. An exosome high-throughput full-automatic extraction instrument according to claim 1, wherein a third support frame (403) is further connected to the second support frame (402) in a sliding manner, a gripping assembly (41) is arranged on the third support frame (403), the gripping assembly (41) comprises a gripping member (411) and a fourth driving member (412), and the fourth driving member (412) drives the gripping member (411) to grip an experimental material.

6. The exosome high-throughput full-automatic extraction instrument according to claim 1, characterized in that the material shifting assembly (51) comprises an operation platform (511), the operation platform (511) is connected to the base (1) in a sliding manner, and a plurality of positioning holes are arranged on the operation platform (511).

7. The exosome high-throughput full-automatic extractor according to claim 6, characterized in that the pressurizing and pressure-maintaining assembly (52) is arranged above one end of the operation platform (511), the pressurizing and pressure-maintaining assembly (52) comprises a support (521), a cover plate (522) and a second cylinder (523); the second cylinder (523) is arranged on the support member (521), the cover plate (522) is connected with the second cylinder (523), and the cover plate (522) is driven by the second cylinder (523) to move up and down in a reciprocating manner.

8. The exosome high-throughput full-automatic extraction instrument according to claim 1, characterized in that the disinfection component (53) comprises a disinfection reagent bottle (532) and an extrusion piece, the disinfection reagent bottle (532) contains disinfection reagent; the extrusion comprises a third cylinder (535) and an extrusion block (534); one end of the extrusion block (534) is connected with the third cylinder (535), and the other end of the extrusion block (534) is connected with the disinfection reagent bottle (532).

9. Exosome high-throughput fully-automatic extractor according to claim 1, characterized in that said consumable collecting mechanism (6) comprises a transport assembly (62), a recovery tank (61) and a storage chamber (63);

the recovery tank (61) is arranged between the placing platform (2) and the operating platform (511);

the transmission assembly (62) comprises a fifth driving piece (621) and a transmission belt (622), the transmission belt (622) is arranged at the bottom of the recycling groove (61), and the transmission belt (622) is communicated with the recycling groove (61) and the storage cavity (63).

10. The exosome high-throughput full-automatic extractor according to claim 9, characterized in that the notch aperture of the recovery tank (61) is gradually reduced from top to bottom.

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