CN215924933U - Nucleic acid extraction and fluorescence PCR detection system - Google Patents

Abstract

The utility model discloses a nucleic acid extraction and fluorescence PCR detection system, which comprises: the device comprises an extraction strip, an extraction strip loading device for loading the extraction strip, a sample rack loading device for loading and unloading a sample, an extraction strip transfer gripper for driving the extraction strip to move to a preset position, a nucleic acid extraction device for purifying the sample to obtain nucleic acid, a PCR detection device for performing fluorescence PCR detection on the nucleic acid and a control device; the extraction strip loading device, the sample frame loading device, the extraction strip transferring gripper, the nucleic acid extraction device and the PCR detection device are all connected with the control device. The method can solve the problems of poor flexibility and low detection efficiency of a nucleic acid extraction detection system, and enables the sample detection process to be more flexible and convenient.

Description

Nucleic acid extraction and fluorescence PCR detection system

Technical Field

The utility model relates to the technical field of molecular diagnosis, in particular to a nucleic acid extraction and fluorescence PCR detection system.

Background

The molecular diagnosis has high sensitivity, good specificity, timeliness and convenience, is widely applied to clinic, and puts higher requirements on the automation degree, flexibility and flux of an extraction system in the diagnosis of certain projects.

At present, a batch sample introduction mode is mostly adopted in a nucleic acid extraction system on the market, meanwhile, detection items are few, manual modes such as sample supply, reagent strip extraction supply and consumable material abandonment are mostly adopted, the market demand of items with a large diagnosis quantity is not met, in the conventional nucleic acid extraction system, a sample to be detected needs to be coded and tested in advance or manually scanned with a bar code, a test position is manually placed, the test flow is complex, and the manual labor intensity is high. Moreover, the batch sample introduction mode can cause that the detection operation of the new sample cannot be performed in time when the new sample needs to be detected, and the detection operation of the new sample can be performed only after the detection of the previous batch of samples is completed, which can cause that the flexibility of the system is poor and the detection efficiency is low.

Therefore, how to improve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction detection system is a technical problem to be solved urgently by those skilled in the art.

SUMMERY OF THE UTILITY MODEL

In view of this, the present invention provides a nucleic acid extraction and fluorescence PCR detection system, which can effectively solve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction detection system, so that the sample detection process is more flexible and convenient.

In order to achieve the above purpose, the utility model provides the following technical scheme:

a nucleic acid extraction and fluorescence PCR detection system, comprising: the device comprises an extraction strip, an extraction strip loading device, a sample rack loading device, an extraction strip transfer gripper, a nucleic acid extraction device, a PCR detection device and a control device, wherein the extraction strip loading device is used for loading the extraction strip and has the function of adding the extraction strip on line;

the extraction strip loading device, the sample rack loading device, the extraction strip transfer gripper, the nucleic acid extraction device and the PCR detection device are all connected with the control device.

Preferably, the extraction strip loading device includes: the channel and a pushing hand device used for pushing the extraction strip to move in the channel;

one end of the channel is a grabbing position of the extracting strip, the pushing device can move transversely, and the pushing device is connected with the control device.

Preferably, the grabbing part is provided with a lifting device which can lift and is used for separating the extraction strips, and the lifting device is connected with the control device.

Preferably, the sample rack loading device includes: the system comprises a sample rack for loading samples, a loading pushing handle, an emergency treatment pushing handle, a scanner, a transfer pushing handle and a loading pushing handle, wherein the transfer pushing handle is used for transferring the samples to the scanning position of the scanner and moving the scanned samples to the sampling position, and the loading pushing handle is used for transporting the sampled samples back to a sample recovery area;

the loading pushing handle, the emergency treatment pushing handle, the scanner, the transfer pushing handle and the loading pushing handle are all connected with the control device.

Preferably, the extraction strip transfer gripper comprises: the foldable gripper device is used for gripping the extraction strip, the gripper lifting device is used for driving the gripper lifting device to move up and down, and the gripper transverse moving device is used for driving the gripper lifting device to move transversely;

the gripper device, the gripper lifting device and the gripper transverse moving device are all connected with the control device.

Preferably, the nucleic acid extraction apparatus comprises: the device comprises a sealing membrane puncturing device for puncturing a sealing membrane of the extraction strip, a filling device for adding the sample to be detected to the extraction strip, a reagent needle, a magnetic bead cleaning device, a high-temperature dissociation device for performing high-temperature incubation operation on the sample, and a purification liquid magnetic absorption device for performing magnetic absorption and purification on the sample to obtain nucleic acid purification liquid;

the reagent needle is used for adding a corresponding reagent into the extraction strip or the PCR tube and adding the nucleic acid purification solution of the extraction strip into the PCR tube.

Preferably, the membrane sealing and puncturing device comprises: the driving assembly is connected with the puncture device, when the puncture device moves to a puncture station, the puncture device punctures the sealing film of the extraction strip, and the driving assembly is connected with the control device.

Preferably, the filling device comprises: a sample needle for sucking the sample and a sample moving device for driving the sample needle to perform spatial movement, wherein the sample needle is connected with the sample moving device, and the sample moving device is connected with the control device.

Preferably, the high temperature dissociation device comprises an incubation heating block for heating the reaction chamber of the extraction strip and a heating block driving device, and the incubation heating block is connected with the control device.

Preferably, the magnetic bead washing device includes: the washing liquid needle, a washing liquid driving device for driving the washing liquid needle to move spatially, a magnet device for selectively applying a magnetic field to the reaction bin of the extraction strip, and a magnet driving device for driving the magnet device to rotate;

the magnet driving device is connected with the magnet device, and the lotion needle is connected with the lotion driving device;

the magnet driving device and the lotion driving device are both connected with the control device.

Preferably, the device also comprises a refrigerated reagent supply device with a refrigeration function for supplying different reagents.

Preferably, the device further comprises a discarding device for recycling the discarded extraction strips, and the discarding device is connected with the control device.

Preferably, the PCR detection device comprises a fluorescent PCR detection device for performing fluorescent PCR detection of different items on the sealed PCR tube;

the fluorescence PCR detection device is connected with the control device.

Preferably, the fluorescent PCR detection apparatus includes: the device comprises a shell, a photometric component and a temperature control component, wherein the shell is provided with a plurality of test positions for placing the PCR tubes, the temperature control component is correspondingly arranged at each test position so as to enable the test positions to be mutually independent to carry out temperature lifting control, and the photometric component and the temperature control component are both connected with a control device;

the light measuring assembly comprises a light source assembly for providing a test light source and a driving part for driving the light source assembly to move; the number of the light source components is multiple, the types of light sources provided by the light source components are different, and the light source components can irradiate the test positions under the driving of the driving part.

When the nucleic acid extraction and fluorescence PCR detection system provided by the utility model is used, firstly, the control device can control the extraction strip loading device to automatically load the extraction strip, control the sample frame loading device to load a sample to be detected, then control the operation of the nucleic acid extraction device, add the sample, reagents required by reaction and the like into the extraction strip, move the extraction strip, incubate, clean and other purification operations on the sample and the reagents in the extraction strip to obtain a nucleic acid purification solution, finally, perform fluorescence PCR detection on the nucleic acid by using the PCR detection device, and feed back the detection result to the control device in real time. The position of the extraction strip among the devices can be moved by the extraction strip transfer gripper, and the extraction strip can be spatially moved by controlling the extraction strip transfer gripper, so that the extraction strip can reach a required position to perform corresponding operation reaction and the like. Thus, the automatic operation of nucleic acid extraction and detection can be realized, the manual operation intensity can be reduced, and the detection efficiency of the system can be improved.

Because the extraction strip loading device of the device has the function of adding the extraction strips on line, the previous extraction strips are immediately interrupted to push the transportation operation when new extraction strips are not required to be added every time, the automation degree and the continuous conveying effect of the extraction strip loading device are favorably improved, and the sample detection efficiency is favorably improved.

In conclusion, the nucleic acid extraction and fluorescence PCR detection system provided by the utility model can effectively solve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction and detection system, so that the sample detection process is more flexible and convenient.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a schematic diagram of a nucleic acid extraction and fluorescence PCR detection system according to the present invention;

FIG. 2 is a schematic view of the structure of an extraction strip;

FIG. 3 is a schematic structural diagram of a pick-up bar loading apparatus;

FIG. 4 is a schematic structural diagram of a sample rack loading device;

FIG. 5 is a schematic diagram of the pick-up bar transfer gripper;

FIG. 6 is a schematic view of the structure of the membrane rupturing device;

FIG. 7 is a schematic view of the structure of a reagent needle;

FIG. 8 is a schematic view of a magnetic bead washing apparatus;

FIG. 9 is a schematic view of the structure of the discarding apparatus;

FIG. 10 is a schematic structural view of a PCR tube transfer device;

FIG. 11 is a schematic structural view of a transfer gripper for PCR tubes;

FIG. 12 is a schematic structural diagram of a fluorescence PCR detection device;

FIG. 13 is a schematic view of the filling device;

FIG. 14 is a schematic view of the structure of a cryogenic cracking unit;

FIG. 15 is a schematic structural view of a pyrolysis apparatus;

FIG. 16 is a schematic view of a magnetic attraction device for purified liquid;

FIG. 17 is a schematic view of the consumable supply apparatus;

FIG. 18 is a schematic diagram of a TIP configuration;

FIG. 19 is a schematic view of the construction of a PCR cap;

FIG. 20 is a schematic view of the structure of a PCR tube;

FIG. 21 is a schematic view showing the configuration of a chilled reagent supply apparatus.

In fig. 1-21:

1 is an extraction strip, 101 is a hanger, 102 is 300ul TIP, 103 is a dissociation reagent, 104 is a label, 105 is a washing solution, 106 is a nucleic acid release solution, 107 is a magnetic bead, 108 is 2ml TIP, 109 is a reaction chamber, 2 is an extraction strip loading device, 201 is a first channel, 202 is a second channel, 203 is a first pusher device, 204 is a second pusher device, 205 is a transfer device, 206 is a loading port, 207 is a grasping site, 208 is a jacking device, 209 is an avoidance hole, 3 is a sample rack loading device, 301 is a sample rack, 302 is a loading pusher, 303 is an emergency pusher, 304 is a scanner, 305 is a transfer pusher, 306 is a loading pusher, 307 is a sampling site, 4 is an extraction strip transfer gripper, 401 is a gripper device, 402 is a gripper lifting device, 403 is a gripper device, 5 is a sample tube, 6 is a purification solution device, 601 is a front and back pusher, 602 is a magnet, 7 is a sealing membrane device, 7 is a magnetic attraction membrane device, 7 is a puncture device, and the sample tube is a magnetic membrane device, 701 is a puncture outfit, 702 is a cleaning device, 703 is a puncture fore-and-aft push hand, 704 is a scanning instrument, 8 is a filling device, 801 is a sample needle, 802 is a sample moving device, 8021 is a sample vertical motion mechanical arm, 8022 is a sample fore-and-aft motion mechanical arm, 803 is a first fore-and-aft push hand, 9 is a low-temperature lysis device, 901 is a second fore-and-aft push hand, 902 is an incubation heating block, 10 is a magnetic bead cleaning device, 1001 is a wash needle, 1002 is a wash liquid driving device, 1003 is a magnet device, 1004 is a fourth fore-and-aft push hand, 11 is a high-temperature dissociation device, 1101 is a third fore-and-aft push hand, 12 is a reagent needle, 1201 is a needle device, 1202 is a needle driving device, 12021 is a needle horizontal motion mechanical arm, 12022 is a needle fore-and-aft motion mechanical arm, 12023 is a needle vertical motion mechanical arm, 13 is a fluorescence PCR detection device, 1301 is a photometric component, 1302 is a pressure cylinder, 1303 is a test position, 1304, a pressure cylinder is placed at a place, 1305 is PCR tube abandon port, 14 is PCR tube transfer device, 1401 is PCR tube placing hole, 1402 is transfer block, 15 is PCR tube transfer gripper, 1501 is PCR gripper, 1502 is PCR horizontal motion mechanical arm, 1503 is PCR forward and backward motion mechanical arm, 1504 is PCR vertical motion mechanical arm, 16 is cold storage reagent supply device, 1601 is pipette hole, 17 is consumable supply device, 1701 is TIP consumable rack, 1702 is PCR consumable rack, 1703 is TIP, 1704 is PCR tube, 1705 is PCR cover, 18 is abandon device, 1801 is waste liquid needle, 1802 is open and close gripper, 1803 is fifth forward and backward pushing hand.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The core of the utility model is to provide a nucleic acid extraction and fluorescence PCR detection system, which can effectively solve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction detection system, and enables the sample detection process to be more flexible and convenient.

Please refer to fig. 1 to fig. 21.

The embodiment provides a nucleic acid extraction and fluorescence PCR detection system, which comprises: the device comprises an extraction strip 1, an extraction strip loading device 2, a sample rack loading device 3, an extraction strip transfer gripper 4, a nucleic acid extraction device, a PCR detection device and a control device, wherein the extraction strip loading device 2 is used for loading the extraction strip 1 and has the function of adding the extraction strip 1 on line, the sample rack loading device 3 is used for loading and unloading a single sample or a plurality of samples, the extraction strip transfer gripper 4 is used for driving the extraction strip 1 to move to a preset position, the nucleic acid extraction device is used for purifying the samples to obtain nucleic acid, and the PCR detection device and the control device are used for carrying out fluorescence PCR detection on the nucleic acid; the extraction strip loading device 2, the sample rack loading device 3, the extraction strip transfer gripper 4, the nucleic acid extraction device and the PCR detection device are all connected with the control device.

The extraction strip 1 has a structure as shown in FIG. 2, and includes a plurality of reagent wells for holding reagents, and a plurality of empty wells, wherein the types of reagents held on the extraction strip 1 include a dissociation reagent 103, a washing solution 105, a nucleic acid release solution 106, magnetic beads 107, and the like, and a sealing film is provided on the top of the reagent wells, and therefore, when the extraction strip 1 is subjected to an addition operation, a pricking operation must be performed in advance. The end of the extraction strip 1 is provided with a reaction bin 109, the extraction strip 1 is also provided with 2ml of TIP108 and 300ul of TIP102, inner holes above the two TIPs can be matched with the same TIP adapter, two ends of the extraction strip 1 are provided with hangers 101, and in addition, the extraction strip 1 is provided with a recognizable mark 104.

In the actual operation process, the structures, sizes, positions, and the like of the extraction strip loading device 2, the sample rack loading device 3, the extraction strip transfer gripper 4, the nucleic acid extraction device, the PCR detection device, and the control device can be determined according to actual conditions and actual requirements.

When the nucleic acid extraction and fluorescence PCR detection system provided by the utility model is used, firstly, the control device can control the extraction strip loading device 2 to automatically load the extraction strip 1, control the sample rack loading device 3 to load a sample to be detected, then control the operation of the nucleic acid extraction device, add the sample, reagents required by reaction and the like into the extraction strip 1, move the extraction strip 1, incubate, clean and other purification operations on the sample and the reagents in the extraction strip 1 to obtain a nucleic acid purification solution, finally, perform fluorescence PCR detection on the nucleic acid by using the PCR detection device, and feed back the detection result to the control device in real time. The position of the extraction bar 1 among the devices can be moved by the extraction bar transfer gripper 4, and the spatial movement of the extraction bar 1 can be realized by controlling the extraction bar transfer gripper 4, so that the extraction bar 1 reaches a required position to perform corresponding operation reaction and the like. Thus, the automatic operation of nucleic acid extraction and detection can be realized, the manual operation intensity can be reduced, and the detection efficiency of the system can be improved.

Because the extraction strip loading device 2 of the device has the function of adding the extraction strips 1 on line, namely when a new extraction strip 1 is not required to be added every time, the previous extraction strip 1 is immediately interrupted to push the transportation operation, and the automation degree and the continuous conveying effect of the extraction strip loading device 2 are favorably improved, so that the sample detection efficiency is favorably improved.

In conclusion, the nucleic acid extraction and fluorescence PCR detection system provided by the utility model can effectively solve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction and detection system, so that the sample detection process is more flexible and convenient.

On the basis of the above embodiment, it is preferable that the extraction bar loading means 2 includes: the channel and a pushing hand device used for pushing the extraction strip 1 to move in the channel; one end of the channel is a grabbing part 207 for taking out the strips 1, the pushing device can move transversely, and the pushing device is connected with the control device.

Preferably, the channels may include a first channel 201 and a second channel 202 arranged in parallel with the first channel 201 to facilitate placement of more extraction strips 1. A first pushing hand device 203 may be arranged in the first channel 201, a second pushing hand device 204 may be arranged in the second channel 202, the first pushing hand device 203 is used for pushing the extraction strip 1 to move in the first channel 201, the second pushing hand device 204 is used for pushing the extraction strip 1 to move in the second channel 202, and a transfer device 205 may be arranged for transferring the extraction strip 1 from the first channel 201 to the second channel 202, the transfer device 205 may be arranged at the other end of the first channel 201 and the second channel 202, the first pushing hand device 203, the second pushing hand device 204 and the transfer device 205 are all connected with the control device.

Preferably, both the first pusher means 203 and the second pusher means 204 are movable laterally and in elevation, in order to make the placement of the extraction bar 1 more randomized. Of course, the pushing device may not have a lifting function, for example, a sensor may be provided, so that the sensor may be triggered each time the extraction bar 1 is newly added, and the pushing device returns to the initial position to perform the loading operation. A draw bar loading device 2 may be arranged in front of the system to facilitate placement of the draw bars 1. Any position of the first channel 201 and the second channel 202 can be used as a loading port 206 of the extraction strip 1.

For example, the left end of the second channel 202 may be set as the grasping place 207 of the extraction bar 1, and the transfer device 205 may be set at the right ends of the first channel 201 and the second channel 202.

In the actual application process, the shapes, structures, sizes, positions, etc. of the extraction bar 1, the first channel 201, the second channel 202, the first pushing hand device 203, the second pushing hand device 204, and the transfer device 205 can be determined according to actual conditions and actual requirements.

When the extraction bar 1 is loaded by the extraction bar loading device 2, firstly, the extraction bar 1 is loaded at the loading port 206 of the first channel 201, then, the first pushing hand device 203 can push the extraction bar 1 to move in the first channel 201 in the process of transverse movement, when the extraction bar 1 reaches the right end position of the first channel 201, the extraction bar 1 can be transferred into the second channel 202 through the transfer device 205, then, the extraction bar 1 is transferred to the left end from the right end of the second channel 202 through the second pushing hand device 204, and when the extraction bar 1 reaches the grabbing position 207 of the second channel 202, the extraction bar transfer gripper 4 can grab the extraction bar 1, so as to conveniently carry out nucleic acid extraction operation on the extraction bar 1.

The moving, conveying and storing operations of the extraction bar 1 can be realized in the first channel 201 and the second channel 202, and the first pushing device 203 and the second pushing device 204 of the device can perform lifting movement in addition to the transverse moving operation, so that when the extraction bar 1 is newly added in the first channel 201 or the second channel 202, the first pushing device 203 or the second pushing device 204 can continue the current action of transversely pushing the extraction bar 1 without immediately returning to the initial position.

After the operation of adding the extraction bar 1 is finished, the first pushing device 203 or the second pushing device 204 is controlled to descend first to avoid collision with the extraction bar 1, then the first pushing device 203 or the second pushing device 204 is moved to the initial position, and when the newly added extraction bar 1 needs to be pushed again, the first pushing device 203 or the second pushing device 204 is controlled to ascend, and the newly added extraction bar 1 is pushed again to move. Therefore, the device can realize the online adding operation of the extraction strips 1, and the prior pushing and transporting operation is immediately interrupted without adding new extraction strips 1 every time, thereby being beneficial to improving the automation degree and the using effect of the device.

Preferably, the grabbing part 207 is provided with a lifting device 208 capable of lifting and used for separating the extraction bar 1, and the lifting device 208 is connected with the control device.

It should be noted that the jacking device 208 is arranged at the grabbing position 207, so as to separate the extraction bar 1 which is tightly attached front and back, and the extraction bar 1 jacked by the jacking device 208 can be grabbed by the extraction bar transfer gripper 4 more conveniently and accurately.

It should be noted that, if the first pushing handle device 203 and the second pushing handle device 204 can move both laterally and vertically, an avoiding hole 209 for allowing the first pushing handle device 203 and the second pushing handle device 204 to pass through after the first pushing handle device 203 and the second pushing handle device 204 are lowered may be provided on the extraction bar 1. This ensures that the first pusher 203 and the second pusher 204 can smoothly avoid the extraction bar 1 when they descend, so as to avoid collision or overturn of the extraction bar 1.

Preferably, the sample rack loading device 3 includes: a sample rack 301 for loading single or multiple samples, a loading pusher 302, an emergency pusher 303, a scanner 304, a transfer pusher 305, and a load-out pusher 306, the transfer pusher 305 being used to transfer the samples to the scanning site of the scanner 304 and move the scanned samples to the sampling site 307, the load-out pusher 306 being used to transport the sampled samples back to the sample recovery area; the loading pusher 302, emergency pusher 303, scanner 304, transfer pusher 305, and load pusher 306 are all connected to the control device. The sample rack loading device 3 may be arranged in front of the present system to facilitate placement of the sample rack 301. Wherein, a plurality of sample tubes 5 can be placed on the sample rack 301, and the sample tubes 5 are used for placing sample liquid.

It should be noted that the transfer pusher 305 may transfer the sample rack 301 loaded by the loading pusher 302 or the emergency treatment pusher 303 to a barcode scanning position of the scanner 304, and after the sample tube 5 completes the scanning operation, the sample rack 301 is moved, so that the sample tubes 5 on the sample rack 301 are sequentially moved to a sampling position 307, so that after the subsequent filling device 8 samples the sample tubes 5, the sample rack 301 is moved to a return position, and finally, the loading pusher 306 is used to push the sample rack 301 to a sample recovery area. When some samples need emergency detection, the samples can be put into an emergency medical operator 303 for queue-insertion detection operation. When a new sample to be detected needs to be detected, the sample to be detected can be placed on the sample rack loading device at any time, and the detection operation is not required to be carried out after all the previous batch of preset samples are detected, so that the sample detection efficiency and the use flexibility are improved.

The shape, structure, size, material, position, etc. of the sample rack 301, the sample tube 5, the loading pusher 302, the emergency pusher 303, the scanner 304, the transferring pusher 305, and the loading pusher 306 can be determined according to actual conditions and actual requirements during actual operation.

On the basis of the above embodiment, it is preferable that the extraction bar transfer grip 4 includes: the foldable gripper device 401 is used for gripping the extraction bar 1, the gripper lifting device 402 is used for driving the gripper lifting device 401 to move up and down, and the gripper transverse moving device 403 is used for driving the gripper lifting device 402 to move transversely, the gripper device 401 is arranged on the gripper lifting device 402, and the gripper lifting device 402 is connected with the gripper transverse moving device 403 in a sliding mode; the gripper 401, the gripper lifting device 402 and the gripper traverse device 403 are connected to the control device. Therefore, by controlling the opening and closing of the gripper 401 and the spatial movement of the gripper 401, the extraction bar 1 can be effectively gripped, and the extraction bar 1 is driven to move, so that the extraction bar 1 reaches a required position to perform corresponding operation.

It should be noted that the extraction strip transfer gripper 4 may be disposed in front of the sealing membrane puncturing device 7, the filling device 8, the low-temperature lysis device 9, the magnetic bead cleaning device 10, the high-temperature dissociation device 11, the purified liquid magnetic attracting device 6, and the discarding device 18. The extraction strip transfer gripper 4 grabs the extraction strip 1 from the grabbing position of the extraction strip 1 on the extraction strip loading device 2, and transfers the extraction strip 1 to the front of the sealing film puncturing device 7 after the extraction strip 1 is successfully grabbed, so as to facilitate the sealing film puncturing operation. By passing

In the actual operation process, the shapes, structures, sizes, positions, and the like of the hand grip 401, the hand grip lifting device 402, and the hand grip lateral moving device 403 may be determined according to actual conditions and actual requirements.

On the basis of the above embodiment, it is preferable that the nucleic acid extracting apparatus includes: the device comprises a sealing film puncturing device 7 for puncturing a sealing film of the extraction strip 1, a filling device 8 for adding a sample to be detected to the extraction strip 1, a reagent needle 12, a magnetic bead cleaning device 10, a high-temperature dissociation device 11 for performing high-temperature incubation operation on the sample, and a purification liquid magnetic absorption device 6 for performing magnetic absorption and purification on the sample to obtain a nucleic acid purification liquid; the reagent needle 12 is used to add a corresponding reagent into the extraction strip 1 or the PCR tube 1704 and to add the nucleic acid purification solution of the extraction strip 1 into the PCR tube 1704. The reagent needle 12 may be used to take the PCR cap 1705, snap the PCR cap 1705 to the PCR tube 1704, and transfer the snapped PCR tube 1704.

Preferably, the seal-film puncturing device 7 includes: the puncture device 701 comprises a puncture device 701 and a driving assembly, wherein the driving assembly is connected with the puncture device 701 to drive the puncture device 701 to move, when the puncture device 701 moves to a puncture station, the puncture device 701 punctures a sealing film of the extraction strip 1, and the driving assembly is connected with a control device. Therefore, the puncture device 701 punctures the sealing film on the extraction strip 1, and the problem that the sealing film on the top of the extraction strip 1 is punctured before the reagent in the extraction strip 1 is taken is effectively solved.

In addition, the sealing membrane puncturing device 7 may further include a cleaning device 702, the cleaning device 702 is connected to the control device, and the cleaning device 702 is configured to clean the puncture device 701 when the puncture device 701 moves to the cleaning station. The puncture device 701 is cleaned by the cleaning device 702, so that the puncture device 701 can be reused, consumables are effectively reduced, and cost of a user is reduced. And the sealing film puncturing device 7 has simple structure, safety and reliability, small system pollution risk and convenient maintenance.

It should be noted that the sealing membrane puncturing device 7 may be disposed near the refrigerated reagent supplying device 16 to facilitate the subsequent addition of the refrigerated reagent. The sealing film puncturing device 7 may be provided with a puncturing front and rear pushing hand 703, which puncturing front and rear pushing hand 703 is adapted to cooperate with the extraction strip transfer grip 4 for enabling a three-dimensional spatial movement of the extraction strip 1. Therefore, the front and rear puncturing push hands 703 can push the extraction bar 1 from the extraction bar transfer hand 4 into the sealing film puncturing device 7, push the extraction bar 1 back to the extraction bar transfer hand 4 after the puncturing operation is completed, and transfer the extraction bar 1 to the front of the filling device 8 by the extraction bar transfer hand 4.

Wherein the sealing-film puncturing device 7 can be provided with a scanning instrument 704 for identifying the marking 104 on the extraction strip 1. When the sealing film of the extraction strip 1 is punctured, the reagent needle 12 can obtain the TIP1703 from the consumable supply device 17, suck the relevant reagent for participating in the reaction from the refrigerated reagent supply device 16, and add the reagent into the reaction bin 109 of the extraction strip 1, and finally, the reagent needle 12 can discard the used TIP1703 for the next operation.

In the actual application process, the shapes, structures, sizes, positions and the like of the puncturing device, the cleaning device 702, the pushing hands 703 before and after puncturing, and the scanning instrument can be determined according to actual conditions and actual requirements.

Preferably, the filling device 8 comprises: a sample needle 801 for sucking a sample and a sample moving device 802 for driving the sample needle 801 to move spatially, the sample needle 801 is connected to the sample moving device 802, and the sample moving device 802 is connected to the control device.

It should be noted that the sample moving device 802 may include a sample back-and-forth movement mechanical arm 8022 for driving the sample needle 801 to move back and forth and a sample vertical movement mechanical arm 8021 for driving the sample needle 801 to move up and down, and in addition, a first back-and-forth pushing hand 803 may be further provided, and the first back-and-forth pushing hand is used in cooperation with the extraction strip transfer gripper 4 to realize the three-dimensional space movement of the extraction strip 1, so as to ensure that the extraction strip 1 can move to the filling position of the filling device 8. Thus, the first front and rear pusher can push the extraction bar 1 from the extraction bar transfer grip 4 into the filling device 8, after the filling operation has been completed, the extraction bar 1 is pushed back into the extraction bar transfer grip 4, and then the extraction bar transfer grip 4 can transfer the extraction bar 1 to the front of the cryogenic cracking device 9.

In addition, it should be noted that the bottom of the sample needle 801 is vertically provided with a TIP adapter for taking 2ml of TIP108 on the extraction strip 1. When the first front and back pushing hands push the extraction strip 1 into the filling device 8, the sample needle 801 can obtain 2ml of TIP108 from the extraction strip 1, the sample needle 801 moves forward to above the sampling place 307 of the sample rack loading device 3, then, the sample moving device 802 drives the sample needle 801 to move to extract a sample to be tested, and the sample is added into the reaction bin 109 of the extraction strip 1, and then, the sample needle 801 extracts a required reagent from a reagent hole on the extraction strip 1 and adds the required reagent into the reaction bin 109, wherein the required reagent comprises: magnetic beads 107 and nucleic acid releasing liquid 106 required by the reaction are pumped and mixed evenly in the reaction chamber 109 to complete the filling operation. Finally, the sample needle 801 withdraws 2ml of TIP108 onto the extraction strip 1.

The shape, structure, size, position, etc. of the sample needle 801 and the sample moving device 802 can be determined according to actual conditions and actual requirements during actual use.

It should be noted that the nucleic acid extraction apparatus may further include a low temperature lysis apparatus 9, because the sample solution generally needs to be subjected to a low temperature incubation operation of the low temperature lysis apparatus 9, the low temperature incubation operation is favorable for binding the nucleic acid with the magnetic bead 107, then the magnetic bead 107 is subjected to a cleaning operation by the magnetic bead cleaning apparatus 10 to remove substances other than the bound nucleic acid and the magnetic bead 107, and then the high temperature incubation operation by the high temperature dissociation apparatus 11 is favorable for separating the nucleic acid from the magnetic bead 107. However, depending on the nucleic acid releasing solution 106, there may be a case where the low-temperature incubation operation by the low-temperature lysis apparatus 9 is unnecessary, and in this case, the extraction strip transfer grip 4 may be controlled to directly transfer the extraction strip 1 from the filling apparatus 8 to the front of the magnetic bead washing apparatus 10 without transferring the extraction strip to the low-temperature lysis apparatus 9.

Preferably, the high temperature dissociation device 11 comprises an incubation heating block 902 for heating the reaction chamber 109 of the extraction strip 1, the incubation heating block 902 being connected to the control device.

It should be noted that a heating block driving device connected to the incubation heating block 902 may be provided, and the heating block driving device is connected to the control device, and the heating block driving device is used to drive the incubation heating block 902 to perform a spatial movement so as to move the heating block closer to or away from the reaction chamber 109.

It should be noted that the structure of the low temperature cracking device 9 is similar to that of the high temperature dissociation device 11, mainly the difference of the heating temperature of the incubation heating block 902 is that the heating temperature of the low temperature cracking device 9 is lower, and the heating temperature of the high temperature dissociation device 11 is higher. When the incubation operation needs to be performed on the reaction chamber 109, the operation of the heating block driving device may be controlled to drive the incubation heating block 902 to be close to the reaction chamber 109, so as to perform the heating operation on the reaction chamber 109, and after the incubation operation is completed, the incubation heating block 902 is controlled to be separated from the reaction chamber 109. During the heating process of the incubation heating block 902, the sample solution releases nucleic acid under the action of the nucleic acid releasing solution 106, and the nucleic acid is attached to the magnetic beads 107.

In addition, it should be noted that the cryogenic cracking device 9 may be configured with a second front and rear pushing hand 901, and the second front and rear pushing hand 901 is used in cooperation with the extraction strip transferring gripper 4 to realize the three-dimensional space motion of the extraction strip 1. Therefore, the second front and rear pushing hands 901 can push the extraction strip 1 from the extraction strip transfer hand 4 into the low temperature lysis device 9, and after the low temperature incubation operation is completed, the extraction strip 1 is pushed back to the extraction strip transfer hand 4, and the extraction strip transfer hand 4 transfers the extraction strip 1 to the front of the magnetic bead washing device 10.

Preferably, the magnetic bead washing apparatus 10 includes: a washing reagent needle 1001, a washing reagent driving device 1002 for driving the washing reagent needle 1001 to move spatially, a magnet device 1003 for selectively applying a magnetic field to the reaction chamber 109 of the extraction strip 1, and a magnet driving device for driving the magnet device 1003 to rotate; the magnet driving device is connected with the magnet device 1003, and the washing liquid needle 1001 is connected with the washing liquid driving device 1002; the magnet drive and the washing liquid drive 1002 are both connected to the control device.

It should be noted that the magnetic bead washing apparatus 10 may be configured with a fourth front and back pushing handle 1004, and the fourth front and back pushing handle 1004 is used in cooperation with the extraction strip transfer hand 4 to realize the three-dimensional spatial movement of the extraction strip 1. Therefore, the fourth back-and-forth pusher 1004 can push the extraction strip 1 from the extraction strip transfer hand 4 into the magnetic bead washing apparatus 10 to perform a magnetic attraction operation. Also, the wash needle 1001 is provided with a TIP adapter in the vertical direction, which is used to take 2ml of TIP108 on the extraction strip 1.

In the magnetic attraction operation, first, 2ml of TIP108 on the extraction strip 1 may be aligned with the wash needle 1001, and the wash needle 1001 is moved downward to take 2ml of TIP 108; then, the extraction strip 1 is moved to a magnetic attraction position, one of the first magnet and the second magnet is rotated to the magnetic attraction position by rotating the magnet device 1003, so that the magnetic beads 107 attached with nucleic acids in the reaction chamber 109 of the extraction strip 1 are accumulated on the side wall of the reaction chamber 109 under the action of magnetic force, when magnetic attraction is performed, the reaction chamber 109 of the extraction strip 1 is positioned below the washing liquid needle 1001, the washing liquid needle 1001 downwards sucks the liquid in the reaction chamber 109, the magnetic beads 107 are left on the inner wall of the reaction chamber 109 under the action of magnetic force, and then the magnet is rotated to be away from the magnetic attraction position and avoid the movement space of the extraction strip 1; then, the extraction strip 1 is pushed to make the empty bin on the extraction strip 1 aligned with the lotion needle 1001, and the lotion needle 1001 spits out the liquid sucked in 2ml of TIP 108; pushing the extraction strip 1 to make the reagent bin on the extraction strip 1, where the lotion 105 is placed, align with the lotion needle 1001, and the lotion needle 1001 sucks the lotion 105; pushing the extraction strip 1 to align the reaction chamber 109 on the extraction strip 1 with the washing needle 1001, adding 2ml of washing solution 105 in the TIP108 into the reaction chamber 109 by the washing needle 1001, and sucking and stirring the magnetic beads 107 and the washing solution 105 in the reaction chamber 109; then, one of the first magnet and the second magnet is turned to a magnetic attraction position, so that the magnetic beads 107 with the nucleic acid attached in the reaction chamber 109 of the extraction strip 1 are accumulated on the side wall of the reaction chamber 109 under the action of magnetic force; the washing liquid needle 1001 downwards pumps away the liquid in the reaction bin 109, the magnetic beads 107 are left on the inner wall of the reaction bin 109 under the action of magnetic force, and then the magnet is rotated to be away from the magnetic attraction position and avoid the movement space of the extraction strip 1; pushing the extraction strip 1 again to make the empty bin on the extraction strip 1 aligned with the lotion needle 1001, and discharging the liquid sucked in 2ml of TIP108 by the lotion needle 1001; then, the magnetic beads 107 can be cleaned for multiple times according to the requirements of the test items, the cleaned waste liquid is discharged to an empty bin on the extraction strip 1, and the cleaned magnetic beads 107 are left in the reaction bin 109; after the magnetic beads 107 are cleaned, pushing the extraction strip 1 to align the placement position of 2ml of TIP108 on the extraction strip 1 with the washing liquid needle 1001, and withdrawing 2ml of TIP108 onto the extraction strip 1 by the washing liquid needle 1001; then the extraction strip 1 is pushed, so that the 300ul TIP102 on the extraction strip 1 is placed at the position of the lotion needle 1001, and the lotion needle 1001 downwards obtains the 300ul TIP 102; continuing to push the extraction strip 1 to align the dissociation reagent 103 on the extraction strip 1 with the washing needle 1001, sucking the dissociation reagent 103 by the washing needle 1001, and separating the nucleic acid attached to the magnetic beads 107 from the magnetic beads 107 by the dissociation reagent 103; then, the extraction strip 1 is pushed again, the reaction bin 109 on the extraction strip 1 is aligned to the washing liquid needle 1001, the washing liquid needle 1001 adds 300ul of the dissociation reagent 103 in the TIP102 into the reaction bin 109, and the liquid in the reaction bin 109 is sucked, pumped and uniformly mixed; the extraction strip 1 is pushed again, so that the 300ul TIP102 on the extraction strip 1 is positioned at the lotion needle 1001, and the lotion needle 1001 withdraws the 300ul TIP102 on the extraction strip 1, thereby completing the whole magnetic attraction operation process.

After the magnetic attraction operation is completed, the fourth front and rear pushing handle 1004 pushes the extraction bar 1 back to the extraction bar transferring handle 4, and the extraction bar transferring handle 4 transfers the extraction bar 1 to the front of the high temperature dissociation device 11.

Preferably, the high temperature dissociation device 11 may be provided with a third front and rear push handle 1101, the third front and rear push handle 1101 being adapted to cooperate with the extraction strip transfer grip 4 to effect a three-dimensional spatial movement of the extraction strip 1. Therefore, the third front-back pushing handle 1101 can push the extraction strip 1 from the extraction strip transfer grip 4 to the high temperature dissociation device 11 for high temperature incubation, under the action of the dissociation reagent 103, the nucleic acid attached to the magnetic beads 107 can be separated from the magnetic beads 107 by the high temperature incubation for a certain period of time, after the high temperature incubation operation is completed, the extraction strip 1 is pushed back to the extraction strip transfer grip 4, and the extraction strip 1 is transferred by the extraction strip transfer grip 4 to the front of the magnetic absorption device 6 for the purification solution.

Preferably, the purification liquid magnetic attraction device 6 may be disposed within a movement range of the reagent needle 12 to facilitate the reagent needle 12 to suck the purification liquid in the reaction chamber 109 of the extraction strip 1. The purification liquid magnetic attraction means 6 may be provided with a front and rear purification pusher 601 and a magnet 602. Wherein, the front and rear purification pushing hands 601 are used in cooperation with the extraction strip transfer hand 4 to realize the three-dimensional space motion of the extraction strip 1. Therefore, the front and rear purification pusher 601 can push the extraction bar 1 from the extraction bar transfer gripper 4 into the magnetic purification liquid suction device 6 for purification operation, and after the purification operation is completed, push the extraction bar 1 back to the extraction bar transfer gripper 4, and the extraction bar transfer gripper 4 transfers the extraction bar 1 to the front of the discarding device 18. The magnet 602 is used for applying a magnetic force to the reaction chamber 109 of the extraction strip 1, so that the magnetic beads 107 in the reaction chamber 109 are accumulated on the side wall of the reaction chamber 109 under the action of the magnetic force, so that the nucleic acid is left in the liquid, and thus the nucleic acid purification liquid required for detection is obtained.

In addition to the above embodiments, it is preferable that the reagent supplying apparatus further includes a refrigerated reagent supplying device 16 having a refrigerating function for supplying different reagents.

The refrigerated reagent supplying apparatus 16 is provided with a cooling function to better hold the reagent to be refrigerated, and a liquid sucking hole 1601 may be provided at a top portion of the refrigerated reagent supplying apparatus 16, the liquid sucking hole 1601 being matched with a size of the reagent needle 12 so that the reagent needle 12 sucks the reagent.

Preferably, the system may further include a consumable supply device 17 for supplying the TIP1703 and the PCR tube 1704 and the PCR cover 1705, the consumable supply device 17 being provided with a TIP consumable rack 1701 for placing the TIP1703 and a PCR consumable rack 1702 for placing the PCR tube 1704 and the PCR cover 1705.

Preferably, the TIP1703 and the PCR cap 1705 are provided with internal holes above each other, which are matched with the size of the reagent needle 12, so that the reagent needle 12 can take the TIP1703 and the PCR cap 1705. Wherein the inner holes above the TIP1703 and the PCR cap 1705 can be matched with the TIP adapter holes of the reagent needle 12, so that the reagent needle 12 can take the TIP1703 and take the PCR cap 1705.

In addition, it should be added that the reagent needle 12 can adopt a structure of a horizontal, back and forth and vertical three-dimensional motion mechanical arm to realize spatial motion, the reagent needle 12 can be provided with a TIP adapter in the vertical direction, the TIP adapter is used for acquiring the TIP1703 or the PCR cover 1705, and the motion range of the reagent needle 12 is covered with: after the TIP1703 is obtained by the reagent needle 12, reagents on the refrigerated reagent supply device 16 may be added to the reaction chamber 109 or the PCR tube 1704 of the extraction strip 1, the nucleic acid purification solution in the reaction chamber 109 may be added to the PCR tube 1704 to obtain the PCR cap 1705, the PCR cap 1705 may be sealed with the PCR tube 1704, and the sealed PCR tube 1704 may be transferred to the PCR tube transfer device 14.

For example, the reagent needle 12 may take the TIP1703 from the consumable supply device 17, aspirate the nucleic acid purification solution from the reaction chamber 109 of the extraction strip 1, add the nucleic acid purification solution to the PCR tube 1704 in the consumable supply device 17, and discard the TIP1703 from the TIP discard port. After the TIP1703 is taken out from the consumable supply device 17, the reagent needle 12 sucks a reagent necessary for reaction from the cold storage reagent supply device 16, adds the reagent into the same PCR tube 1704, and discards the TIP1703 from the TIP disposal port. Then, the reagent needle 12 takes the PCR cap 1705 from the consumable supply device 17, and snaps the PCR cap 1705 onto the PCR tube 1704 to which the nucleic acid purification solution and the reagent have been added. Finally, the reagent needle 12 transfers the sealed PCR tube.

Preferably, the reagent needle 12 may include a needle unit 1201 and a needle driving unit for driving the needle unit 1201 to perform a spatial movement, and both the needle unit 1201 and the needle driving unit are connected to the control unit. The needle driving device may include a needle horizontal movement robot 12021 for horizontally moving the needle unit 1201, a needle forward and backward movement robot 12022 for forward and backward moving the needle unit 1201, and a needle vertical movement robot 12023 for vertically moving the needle unit 1201, so as to realize three-dimensional spatial movement of the needle unit 1201.

In addition to the above-described embodiments, it is preferable that a discarding device 18 for recovering the discarded extraction strips 1 is further included, and the discarding device 18 is connected to the control device.

Preferably, the discarding device 18 may comprise: the device comprises a waste liquid needle 1801 for sucking waste liquid of the extraction strip 1, an openable and closable gripper 1802 for gripping the waste extraction strip 1, an opening and closing driving device for driving the opening and closing gripper 1802 to open or close, and a lifting driving device for driving the waste liquid needle 1801 to move up and down, wherein the opening and closing driving device and the lifting driving device are connected with a control device.

After the reagent needle 12 has added the nucleic acid purification solution of the extraction strip 1 to the PCR tube 1704, the extraction strip 1 may be discarded. The discarding apparatus 18 may be provided with a fifth front-rear pushing hand 1803. The fifth front-rear pusher 1803 is used in cooperation with the extraction bar transfer gripper 4 to realize three-dimensional spatial movement of the extraction bar 1. Therefore, the fifth front-rear pushing hand 1803 can push the extraction strip 1 from the extraction strip transfer hand 4 into the waste device 18, then the reaction bin 109 and each reagent bin on the extraction strip 1 are sequentially pushed below the waste liquid needle 1801, the waste needle can move in the vertical direction to draw away the residual liquid in the reaction bin 109 and each reagent bin, after the liquid drawing operation is completed, the fifth front-rear pushing hand 1803 pushes the extraction strip 1 to the waste position of the opening and closing hand 1802, and finally, the opening and closing hand 1802 opens to waste the extraction strip 1 at the position.

In addition to the above-mentioned embodiments, it is preferable that the PCR detecting device includes a fluorescent PCR detecting device 13 for performing fluorescent PCR detection of different items on the sealed PCR tube 1704, and the fluorescent PCR detecting device 13 is connected to the control device.

Preferably, the PCR detection device further comprises a PCR tube transmission device 14 and a PCR tube transfer gripper 15, and both the PCR tube transmission device 14 and the PCR tube transfer gripper 15 are connected with the control device. The PCR tube transfer device 14 is used for transferring the sealed PCR tube 1704 to the gripping position of the PCR tube transfer gripper 15, and the PCR tube transfer gripper 15 is used for transferring the PCR tube 1704 from the PCR tube transfer device 14 to the fluorescent PCR detection device 13.

The reagent needle 12 may transfer the sealed PCR tube to the PCR tube transfer device 14. The PCR tube transfer apparatus 14 is provided with a horizontally movable transfer block 1402, and PCR tubes 1704 may be placed in the PCR tube 1704 placement hole 1401 above the transfer block 1402, after which the PCR tubes 1704 may be transferred to the grasping position of the PCR tube transfer gripper 15.

Preferably, the fluorescent PCR detection device 13 includes: the device comprises a shell, a photometric component 1301 and a temperature control component, wherein a plurality of test positions 1303 for placing the PCR tube 1704 are arranged on the shell, the test positions 1303 are correspondingly provided with the temperature control component, so that the test positions 1303 can be independently controlled in temperature rise and fall, and the photometric component 1301 and the temperature control component are connected with a control device; the light measuring assembly 1301 comprises a light source assembly for providing a test light source and a driving component for driving the light source assembly to move; the number of the light source assemblies is multiple, the types of light sources provided by the light source assemblies are different, and the light source assemblies can irradiate the test positions 1303 under the driving of the driving part.

It should be noted that the fluorescence PCR detection apparatus 13 may further include a pressing cylinder 1302 for pressing the PCR tube 1704, the casing is provided with a magnetic attraction component, and the pressing cylinder 1302 is also provided with a magnet piece, so that the pressing cylinder 1302 is attracted to the casing to press the PCR tube. Because each test position 1303 is correspondingly provided with a temperature control component for controlling the temperature of the PCR tube 1704, each test position 1303 can independently control the temperature rise and fall, and thus the simultaneous online detection of different test items is realized.

It should be noted that, both the light measuring unit 1301 and the temperature control unit are connected to the control device, so that the fluorescence PCR detection device 13 can perform real-time fluorescence detection while the temperature is increasing or decreasing. Moreover, each test position 1303 is provided with an independent pressing cylinder 1302, the pressing cylinder 1302 has the function of pressing the PCR tube 1704, the pressing cylinder 1302 is quickly installed and fixed by means of magnetic attraction, and the matching of the PCR tube 1704 and a temperature control component can be ensured to be tight, so that the detection effect is improved.

In addition, the PCR tube transfer grip 15 is movable in three dimensions, and is used to grip the sealed PCR tube 1704 or the pressing cylinder 1302, so as to perform a detection operation in cooperation with the fluorescence PCR detection device 13. The PCR tube transferring gripper 15 may include a PCR gripper 1501 for gripping the sealed PCR tube 1704 and the pressure cylinder 1302, a PCR horizontal movement mechanical arm 1502 for driving the PCR gripper 1501 to move horizontally, a PCR forward and backward movement mechanical arm 1503 for driving the PCR gripper 1501 to move forward and backward, and a PCR vertical movement mechanical arm 1504 for driving the PCR gripper 1501 to move vertically. The PCR gripper 1501 can grip the pressure cylinder 1302 on the fluorescence PCR detection device 13, place the pressure cylinder 1302 at the pressure cylinder placement part 1304, grip the PCR tube 1704 from the PCR tube transport device 14, place the PCR tube 1704 at the test position 1303 on the fluorescence PCR detection device 13 where the pressure cylinder 1302 is opened, grip the pressure cylinder 1302 at the pressure cylinder placement part 1304, place the pressure cylinder 1302 at the pressure cylinder placement part 1704 after the fluorescence PCR detection is completed, grip the detected PCR tube 1704, move the PCR tube 1704 to the PCR tube discard opening 1305 for discarding, grip the pressure cylinder 1302 at the pressure cylinder placement part 1304, and place the pressure cylinder 1302 back to the initial position.

In addition, the nucleic acid extraction and fluorescence PCR detection system provided by the present invention includes a frame, and an extraction strip loading device 2, a sample rack loading device 3, an extraction strip transfer gripper 4, a sealing membrane puncturing device 7, a filling device 8, a low temperature lysis device 9, a magnetic bead cleaning device 10, a high temperature dissociation device 11, a purified liquid magnetic attraction device 6, a waste device 18, a consumable supply device 17, a refrigerated reagent supply device 16, a reagent needle 12, a PCR tube transfer device 14, a PCR tube transfer gripper 15, a fluorescence PCR detection device 13, etc. which are respectively disposed on the frame. The devices are integrated into a system, so that the integration degree of the system is high, and the automatic extraction of nucleic acid and the automatic detection of fluorescence PCR can be realized.

The system realizes automatic loading of the extraction reagent through the extraction strip loading device 2; the automatic loading and unloading of the sample to be tested are realized through the sample rack loading device 3; the automatic extraction operation of nucleic acid is realized through the extraction strip transfer gripper 4, the sealing membrane puncture device 7, the filling device 8, the low-temperature cracking device 9, the magnetic bead cleaning device 10, the high-temperature dissociation device 11, the purified liquid magnetic attraction device 6, the waste device 18, the consumable supply device 17, the refrigerated reagent supply device 16 and the filling device 8; the automatic detection operation of the fluorescence PCR of the nucleic acid is realized through the PCR tube transmission device 14, the PCR tube transfer gripper 15 and the fluorescence PCR detection device 13.

The system supports the on-machine detection of a sample to be detected at any time, is provided with a plurality of independent temperature rise drop photometric devices, supports the on-machine detection of a plurality of items at the same time, and realizes the functions of single random and random on-line detection of the sample to be detected. The detection waiting time of the sample is effectively reduced. In addition, the whole processes of nucleic acid extraction and fluorescence PCR detection of the system are not manually participated, the automation degree is high, manual errors in detection can be effectively avoided, the detection efficiency is improved, and the labor intensity is reduced.

It should be noted that, in this document, reference is made to the first channel 201 and the second channel 202, the first pushing device 203 and the second pushing device 204, the first magnet and the second magnet, the first front and back pushing handle 803 and the second front and back pushing handle 901, the third front and back pushing handle 1101, the fourth front and back pushing handle 1004, and the fifth front and back pushing handle 1803, wherein the first, second, third, fourth, and fifth are only for distinguishing the difference of the positions, and are not sequentially arranged.

It should be noted that the directions and positional relationships indicated by "up and down", "front and back", and the like in the present application are based on the directions and positional relationships shown in the drawings, and are only for the convenience of simplifying the description and facilitating the understanding, but do not indicate or imply that the device or element referred to must have a specific direction, be configured and operated in a specific direction, and thus, should not be construed as limiting the present invention.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. Any combination of all the embodiments provided by the present invention is within the scope of the present invention, and will not be described herein.

The nucleic acid extraction and fluorescence PCR detection system provided by the utility model is described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.

Claims (14)

1. A nucleic acid extraction and fluorescence PCR detection system, characterized by comprising: the device comprises an extraction strip (1), an extraction strip loading device (2) which has the function of adding the extraction strip (1) on line and is used for loading the extraction strip (1), a sample rack loading device (3) used for loading and unloading a sample, an extraction strip transfer gripper (4) used for driving the extraction strip (1) to move to a preset position, a nucleic acid extraction device used for purifying the sample to obtain nucleic acid, a PCR detection device used for carrying out fluorescence PCR detection on the nucleic acid and a control device;

the extraction strip loading device (2), the sample rack loading device (3), the extraction strip transfer gripper (4), the nucleic acid extraction device and the PCR detection device are all connected with the control device.

2. The nucleic acid extraction and fluorescence PCR detection system according to claim 1, wherein the extraction strip loading device (2) comprises: a channel and a pushing hand device for pushing the extraction strip (1) to move in the channel;

one end of the channel is a grabbing part (207) of the extraction bar (1), the pushing device can move transversely, and the pushing device is connected with the control device.

3. The nucleic acid extraction and fluorescence PCR detection system according to claim 2, wherein the grasping portion (207) is provided with a lifting device (208) capable of lifting and descending for separating the extraction strip (1), and the lifting device (208) is connected with the control device.

4. The nucleic acid extraction and fluorescence PCR detection system according to any one of claims 1 to 3, wherein the sample rack loading device (3) comprises: the system comprises a sample rack (301) for loading samples, a loading pushing hand (302), an emergency treatment pushing hand (303), a scanner (304), a transfer pushing hand (305) and a carrying-out pushing hand (306), wherein the transfer pushing hand (305) is used for transferring the samples to a scanning position of the scanner (304) and moving the scanned samples to a sampling position (307), and the carrying-out pushing hand (306) is used for carrying the sampled samples back to a sample recovery area;

the loading pushing hand (302), the emergency treatment pushing hand (303), the scanner (304), the transfer pushing hand (305) and the loading pushing hand (306) are all connected with the control device.

5. The nucleic acid extraction and fluorescence PCR detection system according to any one of claims 1 to 3, wherein the extraction strip transfer gripper (4) comprises: the foldable gripper device (401) is used for gripping the extraction bar (1), the gripper lifting device (402) is used for driving the gripper lifting device (401) to move up and down, and the gripper transverse moving device (403) is used for driving the gripper lifting device (402) to move transversely, the gripper device (401) is arranged on the gripper lifting device (402), and the gripper lifting device (402) is connected with the gripper transverse moving device (403) in a sliding mode;

the hand grip device (401), the hand grip lifting device (402) and the hand grip transverse moving device (403) are all connected with the control device.

6. The nucleic acid extraction and fluorescence PCR detection system according to any one of claims 1 to 3, wherein the nucleic acid extraction device comprises: the device comprises a membrane sealing puncturing device (7) for puncturing a membrane sealing of the extraction strip (1), a filling device (8) for adding the sample to be detected to the extraction strip (1), a reagent needle (12), a magnetic bead cleaning device (10), a high-temperature dissociation device (11) for performing high-temperature incubation operation on the sample, and a purification liquid magnetic absorption device (6) for performing magnetic absorption and purification on the sample to obtain a nucleic acid purification liquid;

the reagent needle (12) is used for adding a corresponding reagent into the extraction strip (1) or the PCR tube (1704) and for adding a nucleic acid purification solution of the extraction strip (1) into the PCR tube (1704).

7. The nucleic acid extraction and fluorescence PCR detection system according to claim 6, wherein the membrane sealing puncturing device (7) comprises: the device comprises a puncture device (701) and a driving assembly, wherein the driving assembly is connected with the puncture device (701) to drive the puncture device (701) to move, when the puncture device (701) moves to a puncture station, the puncture device (701) punctures a sealing film of the extraction strip (1), and the driving assembly is connected with the control device.

8. The nucleic acid extraction and fluorescence PCR detection system according to claim 6, wherein the filling device (8) comprises: a sample needle (801) for sucking the sample and a sample moving device (802) for driving the sample needle (801) to perform spatial movement, wherein the sample needle (801) is connected with the sample moving device (802), and the sample moving device (802) is connected with the control device.

9. The nucleic acid extraction and fluorescence PCR detection system according to claim 6, wherein the high temperature dissociation device (11) comprises an incubation heating block (902) for heating the reaction chamber (109) of the extraction strip (1), the incubation heating block (902) being connected to the control device.

10. The nucleic acid extraction and fluorescence PCR detection system according to claim 6, wherein the magnetic bead washing device (10) comprises: the washing liquid extraction device comprises a washing liquid needle (1001), a washing liquid driving device (1002) for driving the washing liquid needle (1001) to move spatially, a magnet device (1003) for selectively applying a magnetic field to a reaction bin (109) of the extraction strip (1), and a magnet driving device for driving the magnet device (1003) to rotate;

the magnet driving device is connected with the magnet device (1003), and the lotion needle (1001) is connected with the lotion driving device (1002);

the magnet driving device and the lotion driving device (1002) are both connected with the control device.

11. The nucleic acid extraction and fluorescence PCR detection system according to any one of claims 1 to 3, further comprising a chilled reagent supply device (16) having a cooling function for supplying different reagents.

12. The nucleic acid extraction and fluorescence PCR detection system according to any one of claims 1 to 3, further comprising a discarding device (18) for recovering the discarded extraction strip (1); the discarding device (18) is connected to the control device.

13. The nucleic acid extraction and fluorescence PCR detection system according to any one of claims 1 to 3, wherein the PCR detection apparatus includes a fluorescence PCR detection apparatus (13) for performing fluorescence PCR detection of different items on the sealed PCR tube (1704);

the fluorescent PCR detection device (13) is connected with the control device.

14. The nucleic acid extraction and fluorescence PCR detection system according to claim 13, wherein the fluorescence PCR detection device (13) comprises: the device comprises a shell, a photometric component (1301) and a temperature control component, wherein the shell is provided with a plurality of test positions (1303) for placing the PCR tube (1704), the temperature control component is correspondingly arranged at each test position (1303), so that the test positions (1303) are independent of each other to perform temperature rise and fall control, and the photometric component (1301) and the temperature control component are connected with a control device;

the light measuring assembly (1301) comprises a light source assembly for providing a test light source and a driving component for driving the light source assembly to move; the number of the light source components is multiple, the types of light sources which can be provided by the light source components are different, and the light source components can irradiate the test positions (1303) under the driving of the driving part.

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