CN115572673A

CN115572673A - Full-automatic sample library preparation system and application

Info

Publication number: CN115572673A
Application number: CN202211187163.6A
Authority: CN
Inventors: 王小锐; 昌雄; 刘良勇; 许腾; 李永军
Original assignee: Beijing Weiyuan Medical Laboratory Co ltd; Guangzhou Vision Gene Technology Co ltd; Guangzhou Weiyuan Intelligent Manufacturing Technology Co ltd; Guangzhou Weiyuan Medical Laboratory Co ltd; Guangzhou Weiyuan Medical Technology Co ltd; Nanjing Weiyuan Medical Laboratory Co ltd; Shenzhen Weiyuan Medical Technology Co ltd; Weiyuan Shenzhen Medical Research Center Co ltd; Guangzhou Weiyuan Medical Equipment Co ltd
Current assignee: Beijing Weiyuan Medical Laboratory Co ltd; Guangzhou Vision Gene Technology Co ltd; Guangzhou Weiyuan Intelligent Manufacturing Technology Co ltd; Guangzhou Weiyuan Medical Equipment Co ltd; Guangzhou Weiyuan Medical Laboratory Co ltd; Guangzhou Weiyuan Medical Technology Co ltd; Shenzhen Weiyuan Medical Technology Co ltd
Priority date: 2022-09-28
Filing date: 2022-09-28
Publication date: 2023-01-06

Abstract

The invention relates to a full-automatic sample library preparation system and application, and belongs to the technical field of nucleic acid detection automation equipment. A fully automated sample library preparation system, comprising: the system can complete the transfer of liquid and the movement of pore plates (including pore plates used for tests such as a deep pore plate, a PCR plate, an ELISA plate and the like) through the operation of a liquid transfer mechanical arm and a plate rotating mechanical arm of the operation module on a workbench, and can complete corresponding procedures for samples according to the requirements of nucleic acid detection processes. And the independent compartment-dividing treatment of the circulating temperature control module and the detection module can be realized by opening and closing the temperature control sealing compartment door and the detection sealing compartment door in the compartment-dividing module, so that the cross contamination risk is effectively reduced.

Description

Full-automatic sample library preparation system and application

Technical Field

The invention relates to the technical field of nucleic acid detection automation equipment, in particular to a full-automatic sample library preparation system and application.

Background

With the rapid development of molecular biology technology, genetic biotechnology has been widely applied to clinical detection, medical research, disease prevention and control, food safety and sanitation, and other fields. In the face of complex and various molecular biological technologies such as nucleic acid extraction, PCR reaction, gene library establishment, gene sequencing and the like, a large amount of experiment personnel with biological technologies are required to be invested, and meanwhile aerosol pollution generated in the experiment process also brings great interference to clinical detection.

Therefore, in order to relieve the pressure of experimenters and avoid experimental errors such as false negative and false positive caused by manual operation as much as possible, a full-automatic pipetting station with standard flow, stability, high speed, safety and reliability is urgently needed in the market.

However, conventional pipetting stations have the following problems: 1. the device only has a single pipetting or extracting function, and does not have equipment integrating pipetting and transferring plates, nucleic acid extraction, shaking incubation, PCR reaction and enzyme labeling quantification, so that the complete process of full-automatic gene library building cannot be realized; 2. the existing automatic pipetting workstation is not designed according to the 'bin dividing' type required by a molecular laboratory, a sample area, a consumable area, a reaction area, a waste area and the like are arranged on a working table surface in a staggered mode in a transverse and longitudinal directions, a completely independent bin dividing space for a PCR reaction area, an enzyme labeling quantification area and a waste area is avoided, and higher cross contamination risks are brought to the experimental process.

Moreover, most of liquid transfer workstations lack a high-efficiency filtering anti-pollution system, and aerosol pollution generated in the experimental process is not rapidly and effectively treated.

Disclosure of Invention

Therefore, a need exists for a full-automatic sample library preparation system, which can reduce the risk of cross contamination in the experimental process by the cooperation of independent warehouse-separated working modules, aiming at the problem that the automatic gene library building workstation has the risk of cross contamination.

The invention discloses a full-automatic sample library preparation system, which comprises:

the operation module comprises a liquid transferring mechanical arm, a plate rotating mechanical arm and a workbench, wherein the plate surface of the workbench is used for placing detection reagents and samples, the liquid transferring mechanical arm and the plate rotating mechanical arm are arranged above the workbench, the liquid transferring mechanical arm is used for performing liquid transferring operation on the reagents or the samples placed on the workbench, the plate rotating mechanical arm is used for transferring pore plates placed on the workbench in the vertical and horizontal directions, and the workbench is further provided with a vibration incubation area for placing the pore plates to be vibrated and incubated; a circulating temperature control inlet and a detection inlet are formed in the workbench;

the bin dividing module is arranged below the workbench and comprises a circulating temperature control module and a detection module, the circulating temperature control module comprises a temperature control sealing partition plate, a temperature control piece and a temperature control sealing bin door, the temperature control piece is arranged in a temperature control sealing cavity formed by the temperature control sealing partition plate, and the temperature control sealing bin door is arranged at a position matched with the circulating temperature control inlet; the detection module comprises a detection sealing partition plate, a detection element and a detection sealing bin door, wherein the detection element is arranged in a detection sealing cavity formed by the detection sealing partition plate, and the detection sealing bin door is arranged at a position matched with the detection inlet;

the garbage bin is arranged in a garbage closed cavity formed by the garbage partition plates below the bin dividing module, one end of the garbage channel is opened in the garbage bin, and the other end of the garbage channel is opened in a waste object area of the workbench;

the control system comprises a main control board which is in signal connection or electric connection with the operation module and the bin dividing module so as to control the operation module and the bin dividing module.

According to the full-automatic sample library preparation system, the liquid transfer and the movement of the pore plates (including pore plates used for tests such as deep pore plates, PCR plates, enzyme label plates and the like) can be completed through the operation of the liquid transfer mechanical arm and the plate rotating mechanical arm of the operation module on the workbench, and the corresponding procedures of the samples are completed according to the requirements of a nucleic acid detection process. And the independent compartment-dividing treatment of the circulating temperature control module and the detection module can be realized by opening and closing the temperature control sealed compartment door and the detection sealed compartment door, so that the cross contamination risk is effectively reduced.

Meanwhile, in the full-automatic sample library preparation system, the inlets of the circulating temperature control module and the detection module are arranged below the workbench, and the circulating temperature control inlet and the detection inlet are formed in the workbench, so that the warehouse-dividing sealing treatment is realized, and the working modes that a plate rotating mechanical arm and the like can rotate a plate in a linear direction but cannot perform broken line or curve motion are facilitated.

It can be understood that the temperature control member can be selected from a conventional thermal cycler (ODTC), the detection element can be selected from an enzyme-linked immunosorbent assay (ELIASA) and the like, and the temperature control member can be arranged according to the specific nucleic acid detection requirements; and for the oscillation incubation area, an oscillation system and a heating system are arranged according to the conventional method.

In one embodiment, the circulating temperature control module further comprises a temperature control air draft system, and the air draft system is arranged on the temperature control sealing partition plate and used for exhausting and filtering air in the temperature control sealed cavity.

The technical personnel in the field know that when the ODTC is started, the inside of the ODTC needs to exchange heat to complete the temperature rise and drop circulation, at the moment, the heat is completely dissipated in a temperature control sealed cavity formed by a temperature control sealed clapboard, and when the temperature in the sealed cavity rises to the low point 50 ℃ of the heat circulation temperature of the ODTC, the ODTC is difficult to or cannot reach 50 ℃, and the experimental result is influenced. At this moment, the air draft system is started, and the ODTC air draft system can set the rotating speed of the fan so as to match the experiment requirements. For example, a relatively low rotation speed of about 1500rpm may be selected, and the setting may be made according to actual conditions depending on the air volume of the fan. Accordingly, heat generated by ODTC thermal cycle is taken away by the air exhaust system, and the temperature rising and falling efficiency of ODTC can be effectively improved, so that the thermal cycle time is reduced, and the preparation efficiency of the whole library is further improved.

More importantly, after the ODTC process ends, a large amount of aerosol is generated at the instant the ODTC opens the thermal cap. Starting exhaust system this moment, ODTC uncaps the aerosol that produces in the twinkling of an eye and can be taken away by exhaust system, and the effectual accumulation and the settlement of having avoided the aerosol, the result of gene sequencing is more reliable.

In one embodiment, the waste module further comprises a waste air draft system, and the air draft system is arranged on the waste partition plate and used for exhausting and filtering air in the waste closed cavity.

Further, when the abandonment module need be maintained, the exhaust system who starts the abandonment module is toward outer convulsions, and 3500rpm is preferred to the fan rotational speed this moment, can be in the inside air current that forms in the past to the back in abandonment module space, then keep apart the aerosol pollution source that filters the discarded rubbish and bring effectively, effectively reduce discarded rubbish and produce cross contamination's risk to the experiment.

In one embodiment, the temperature-controlled sealing bin gate and the detection sealing bin gate each comprise: the device comprises a mounting frame, a sliding rail, a sliding block, a first connecting rod, a second connecting rod, an elastic part, a driving motor, a bin door panel and a sealing part, wherein the mounting frame is fixed on the temperature control sealing partition plate or the detection sealing partition plate; the first connecting rod is fixed in on the slider, the storehouse door plant through two at least second connecting rods with first connecting rod is articulated fixed, the elastic component is located first connecting rod or second connecting rod with between the door plant, work as the storehouse door plant is located open state position under the elastic component spring action, the storehouse door plant constitutes the parallelogram of slope with first connecting rod and two second connecting rods, be equipped with spacing wheel on the door plant of storehouse, be equipped with the locating part on the mounting bracket, work as the storehouse door plant is located close state position, spacing wheel support lean on in the locating part, the storehouse door plant overcomes the elasticity of elastic component moves up, constitutes the rectangle with first connecting rod and two second connecting rods, the sealing member is located storehouse door plant upper surface, work as the storehouse door plant is located close state position, the sealing member be extruded in the workstation lower surface, and encircle circulation accuse temperature entry or detect the entry setting.

The inventor found in previous research that most conventional pipetting stations do not perform partition design experiments according to laboratory requirements, or the so-called partition does not achieve the effect of real partition, and a compact electrically controllable sealing door which achieves better partition sealing effect in a smaller space area may be lacked. According to the temperature control sealing bin door and the detection sealing bin door, through the design of the connecting rod mechanism, the two actions of forward pushing and closing of the bin door can be completed through the control of one motor, and the better sealing effect is achieved.

In one embodiment, the temperature control sealing bin door and the detection sealing bin door further comprise a push plate, a lead screw and a nut, the lead screw is connected with the output end of the driving motor, the nut is sleeved on the lead screw, and the first connecting rod is connected with the nut through the push plate. The matching of the push plate, the lead screw and the nut can improve the stability and reliability of the forward pushing and closing of the bin door.

In one of them embodiment, accuse temperature seals the door and detects sealed door and still includes optical coupling pair and opto-coupler separation blade, the opto-coupler is to locating on the mounting bracket, the opto-coupler separation blade is located on the push pedal, the storehouse door plant is followed slide rail direction removes and has closed state position and open the state position, works as the storehouse door plant is located closed state position, the opto-coupler separation blade is kept away from optical coupling pair works as the storehouse door plant is located open the state position, the opto-coupler separation blade shelters from optical coupling pair. The accessible opto-coupler is to signal identification door state, the control of the control system of being convenient for.

In one embodiment, the circulating temperature control module is further provided with a cleaning cover, and the cleaning cover is fixed on the temperature control sealing partition plate and is opened towards the horizontal direction. When the experiment is completed, the user can manually open the cleaning cap, so as to open the hot cap of the ODTC, spray alcohol or other solvents related to the inhibition of nucleic acid products on the hot cap, and wipe the hot cap by using dust-free paper, so that aerosol products on the ODTC hot cap can be removed.

In one embodiment, an ultraviolet disinfection lamp is arranged in the top of the operation module, the temperature control closed cavity and/or the garbage closed cavity.

When the operation module, the circulating temperature control module or the waste module needs to be cleaned and maintained, the ODTC ultraviolet lamp is arranged above the inside of the independent space, and the ultraviolet lamp can be turned on (the irradiation time of the ultraviolet lamp can be set, and intelligent timing can be realized), so that microorganisms in the space are killed, and a clean air system is effectively formed in the space. Preferably, the ultraviolet lamp is selected to have an irradiation dose of more than 100 000. Mu.W.s/cm ² 。

In one embodiment, the workbench comprises a refrigeration module area and a normal temperature area, and the refrigeration module area is provided with refrigeration equipment for keeping the refrigeration module area at a low temperature. It will be appreciated that the refrigeration module region functions like a conventional refrigerator in a laboratory for storing enzymes and substrates that require low temperature storage for the reaction process, and that conventionally the refrigeration module is typically used at a temperature of 2-8 ℃. The normal temperature zone is mainly used for storing reagents which need to be stored at normal temperature in the reaction process.

In one embodiment, the workbench is provided with a Tip storage area, a refrigeration module area, a normal temperature area, a vibration incubation area, a waste area and a consumable material placing area in sequence from one side to the opposite side. The functional areas of the workbench are arranged according to the positions, which is favorable for the nucleic acid detection process to carry out reagents and reaction steps, and avoids the problems of mutual influence and interference.

In one embodiment, the operation module further comprises a magnetic force adsorption system, the magnetic force adsorption system comprises a magnetic rod, a connecting frame, a guide rail and a motor, the magnetic rod is fixed on the connecting frame, the connecting frame is in sliding connection with the guide rail through the sliding block, and the connecting frame can move up and down along the guide rail under the driving of the motor and drives the magnetic rod to move up and down.

When nucleic acid extraction is carried out to needs, can arrange the deep hole board that contains the sample after the schizolysis in magnetic force adsorption system top (being the magnetic force adsorption zone of workstation promptly), then start driving motor, make the below bar magnet rise and descend along the guide rail slider through the link, reciprocate around the hole site of deep hole board repeatedly, the effect of magnetic attraction that receives the bar magnet that can both be abundant between downthehole liquid level of deep hole board and the bottom like this, the adsorption efficiency of magnetic bead is higher, to the sample of low concentration, the probability of detecting is bigger, thereby the sensitivity of reagent relevance ratio has been improved.

And the magnetic bar is controlled to move up and down to control the height of the magnetic beads adsorbed on the wall of the deep hole plate, when waste liquid in the hole needs to be removed (only pure magnetic beads adsorbed with nucleic acid are left after washing reagent is sucked away), the magnetic beads are moved to a position 2mm away from the bottom surface of the deep hole plate, at the moment, the liquid transfer mechanical arm is moved to a position above the hole where the waste liquid needs to be removed, the gun tip of the liquid transfer pump with TIPS is moved down to a position 0.1-0.2 mm higher than the bottom of the deep hole plate, the waste liquid can be accurately sucked up and cannot be taken away, eluent is added after the waste liquid is removed, the magnetic bar motor is reset, the magnetic beads with the nucleic acid are separated in the eluent, the magnetic bar of the magnetic rack is lifted up and down to move up and down, the magnetic beads dissociated in the reagent are adsorbed on the side wall of the magnetic bar, the liquid transfer mechanical arm is moved to a position above the hole where the liquid with the nucleic acid needs to be removed, the nucleic acid is sucked into the PCR reaction tube, and the extraction of the nucleic acid is completed under the condition that the deep hole plate does not need to be transferred.

In one embodiment, a partition plate is arranged between the bin dividing module and the waste module, the garbage channel comprises an upper channel and a lower channel which are detachably sleeved and connected, the upper channel is fixed on the table top of the workbench through an upper flange, and the lower channel is fixed on the partition plate through a lower flange.

In the conventional nucleic acid detection equipment, a Tips collection box is usually placed on a table board, however, when the kit is used for detection of mNGS and the like, the library building process is very long, more than 800 Tips are needed for statistics, and when the kit is placed on the table board, at least more than 6 plate positions of space are needed for collecting the waste Tips.

However, a new problem exists, a garbage channel leading from a working table to a garbage can at the bottom is easily polluted by Tips in the falling process (the mixture of reagents and nucleic acid is always remained in Tips, and when the Tips fall off, the free-falling body movement residues risk to be scattered around the channel).

The automatic sample library preparation system comprises a first layer of platform surface layer, a second layer of platform surface layer, a third layer of waste module layer, a garbage channel and a partition plate, wherein the first layer of platform surface layer is an operation module layer, the second layer of platform surface layer is a separation module layer, the third layer of platform surface layer is a waste module layer, the garbage channel penetrates through the three layers, the garbage channel is long inevitably, and the channel is difficult to be drawn out manually. When two upper channels and two lower channels are placed, the operation sequence is opposite to the taking sequence.

In one embodiment, the fully automatic sample library preparation system further comprises a main air draft module, the main air draft module comprises a fan and a filtering system, the main air draft module is installed at the top of the operation module, and the air of the workbench is exhausted through the filtering system by the fan.

When this full-automatic sample library preparation system need filter when maintaining, with the equipment door slip closure, open main air drafting module, wherein the fan is toward outer convulsions, fan motor rotational speed is about 3500RPM, operation module's cavity forms from down up air passage this moment, when taking out to the certain extent, interior cavity forms the negative pressure, make and be in slight "vacuum" state in the space, and often follow the high pressure to the low pressure principle according to gas, so gas in the operation space can not blow all around at will, reuse main air drafting module guide air current to keeping away from clean area direction to flow this moment, cooperation ultraviolet disinfection lamp disinfects the adsorption space surface, discharge outside the space after the filtration of 99.999% Hepa system filtration through filtration efficiency, thereby reach splendid filter effect.

The invention also discloses application of the full-automatic sample library preparation system in nucleic acid detection library construction.

The invention also discloses a library construction method, and the full-automatic sample library preparation system comprises the following steps: the sample to be detected is placed on the workbench, the liquid transfer mechanical arm is controlled to absorb sample solution and reagent, the rotating plate mechanical arm is controlled to transfer the pore plate to a required position, and meanwhile, the opening and closing of the temperature control sealing bin door and the detection sealing bin door are controlled to be matched with the transfer of the rotating plate mechanical arm, so that the automatic operation of sample nucleic acid extraction, fragmentation, joint connection, library amplification and library purification is carried out.

Compared with the prior art, the invention has the following beneficial effects:

the invention relates to an animated nucleic acid detection device, which can complete the transfer of liquid and the movement of pore plates (including pore plates used for tests such as deep pore plates, PCR plates, enzyme label plates and the like) by the operation of a liquid-transferring mechanical arm and a rotating plate mechanical arm of an operation module on a workbench, and complete corresponding procedures of samples according to the requirements of a nucleic acid detection process. And the independent bin-dividing treatment of the circulating temperature control module and the detection module can be realized by opening and closing the temperature control sealing bin door and the detection sealing bin door, so that the cross contamination risk is effectively reduced.

Drawings

FIG. 1 is a schematic diagram of a fully automated sample library preparation system.

FIG. 2 is a schematic diagram of a layered and bin-divided structure of a fully automatic sample library preparation system.

FIG. 3 is a schematic view of the stage partition arrangement.

Fig. 4 is a schematic structural diagram of a binning module.

Fig. 5 is an explosion diagram of the temperature-controlled sealing door and the detection sealing door.

FIG. 6 is a schematic diagram of the top side structure of the temperature-controlled sealing door and the detection sealing door.

FIG. 7 is a schematic view of the bottom side structure of the temperature-controlled sealing bin door and the detecting sealing bin door.

FIG. 8 is a schematic diagram of a magnetic adsorption system.

FIG. 9 is a schematic diagram of a discard module.

Figure 10 is a schematic view of the installation of the trash can and trash channel in the waste module.

FIG. 11 is a schematic structural view of the main draft module.

FIG. 12 is a schematic view of the direction of the exhaust flow of the main exhaust module.

FIG. 13 is a back schematic view of a fully automated sample library preparation system.

Wherein: 110. a pipetting mechanical arm; 120. a board rotating mechanical arm; 130. a work table; 140. a magnetic force adsorption zone 140; 141. a magnetic bar; 142. a connecting frame; 143. a guide rail; 144. a motor; 150. a circulating temperature control inlet; 160. detecting an entrance; 170. a waste region; 181. a Tip storage area; 182. refrigerating the module area; 183. a normal temperature zone; 184. a consumable material placement area; 185. a waste liquid zone; 190. oscillating the incubation area; 210. a circulating temperature control module; 211. a temperature control sealing partition plate; 212. a temperature control member (ODTC); 213. a temperature control air draft system; 214. cleaning the cover; 220. a detection module; 221. detecting a sealed bin gate; 231. a mounting frame; 232. a slide rail; 233. a slider; 2341. a first link; 2342. a second link; 2343. a limiting wheel; 2344. a limiting member; 235. an elastic member; 236. a drive motor; 237. a bin gate panel; 238. a seal member; 2391. a nut; 2392. pushing the plate; 241. a light coupling pair; 242. an optical coupling baffle plate; 310. a garbage partition plate; 320. a trash can; 331. an upper channel; 3311. an upper flange; 332. a lower channel; 3321. a lower flange; 340. a waste extraction system; 400. a main air draft module; 410. a fan; 420. a filtration system; 430. a top substrate; 510. an ultraviolet disinfection lamp.

Detailed Description

To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" or "mounted" to another element, it can be directly connected or mounted to the other element or intervening elements may also be present.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Example 1

A fully automated sample library preparation system, as shown in fig. 1, comprising: the system comprises an operation module, a bin dividing module, a waste module, a main air draft module 400 and a control system.

In this embodiment, the operation modules, the bin dividing modules, and the waste modules are arranged in layers from top to bottom, the first layer is the operation module, the second layer is the bin dividing module, the third layer (bottom layer) is the waste module, the first layer and the second layer are separated by the surface of the workbench 130, and the second layer and the third layer are separated by the partition plate, as shown in fig. 2.

The operation module comprises a liquid transferring mechanical arm 110, a plate rotating mechanical arm 120 and a workbench 130, wherein the plate surface of the workbench 130 is used for placing detection reagents and samples, the liquid transferring mechanical arm 110 and the plate rotating mechanical arm 120 are arranged above the workbench 130, the liquid transferring mechanical arm 110 is used for performing liquid transferring operation on the reagents or the samples placed on the workbench 130, the plate rotating mechanical arm 120 is used for transferring pore plates placed on the workbench 130 in the vertical and horizontal directions, the workbench 130 is further provided with a vibration incubation area 190 for placing the pore plates to be vibrated and incubated, and the vibration incubation area 190 is designed conventionally and comprises a heating system and a vibration system; the worktable 130 is provided with a circulating temperature control inlet 150 and a detection inlet 160.

As shown in fig. 3, in this embodiment, the workbench 130 includes a refrigeration module area 182 and a normal temperature area 183, and the refrigeration module area 182 is provided with a refrigeration device for keeping the refrigeration module area 182 at a low temperature. It will be appreciated that the refrigeration module region 182 functions like a conventional refrigerator in a laboratory for storing enzymes and substrates that require low temperature storage for the reaction process, and that conventionally, the refrigeration module is typically used at a temperature of 2-8 ℃. The constant temperature zone 183 is mainly used for storing reagents which need to be stored at normal temperature in the reaction process.

Specifically, the workbench 130 is sequentially provided with a Tip storage area 181, a refrigeration module area 182, a normal temperature area 183, an oscillation incubation area 190, a magnetic adsorption area 140 (under which a magnetic adsorption system is correspondingly provided), a waste area 170 and a consumable material placement area 184 from one side to the opposite side (e.g., facing the workbench from left to right). The Tip storage area 181 is distributed on the left rear side of the surface of the workbench 130, and is mainly used for carrying clean Tip consumables of various specifications required in an experimental process, and preferably generally loads tips of three specifications of 1000ul, 200ul and 50 ul. The normal temperature zone 183 is located on the right side of the refrigeration module zone 182, is mainly used for storing reagents which need to be stored at normal temperature in the reaction process, and is generally designed to be divided into 5 notches for storing more than 5 normal temperature reagents.

In this embodiment, the workbench 130 is further provided with a waste liquid area 185, the waste liquid area 185 is located behind the waste liquid area 170, waste liquid generated in the experiment process is discharged to the waste liquid area 185 through the liquid-transferring mechanical arm 110, preferably, the waste liquid area 185 is provided with a waste liquid barrel with a cover, and the barrel cover is provided with a small section of opening, so that waste liquid discharge is facilitated, and pollution of the waste liquid to the experiment process can be effectively reduced.

In this embodiment, the surface of the worktable 130 may be substantially divided into a clean area and a reaction area, the left three rows are the clean area, and the clean area mainly includes a Tip storage area 181, a refrigeration module area 182, a normal temperature area 183, and a ring temperature control inlet, because the temperature control sealing door corresponding to the ring temperature control inlet is usually in a closed state, and the left clean area usually stores clean tips and reagent samples, a relatively clean area is formed.

The three columns on the right side are reaction areas, which mainly comprise a shaking incubation area 190, a magnetic adsorption area 140, a waste area 170, a waste liquid area 185, a consumable material placing area 184 and a detection inlet 160 (corresponding to a detection sealing bin gate 221), wherein the experimental reaction processes of nucleic acid extraction, pipetting and the like are mainly completed in the areas, and a pollution source easily generated in the reaction process can relatively stay in the reaction areas, so that the isolation of a clean area and the reaction areas is realized.

The functional regions of the worktable 130 are arranged according to the above positions, which is beneficial to the nucleic acid detection process to carry out reagents and reaction steps, and avoids the problems of mutual influence and interference.

It can be understood that the pipetting robot 110 and the rotating plate robot 120 may be conventional apparatuses, and may be specifically adjusted according to actual experimental requirements. In this embodiment, the pipetting robot 110 includes 8 independently operated pipetting pumps, which can respectively realize the control in the three directions of X, Y and Z, and mainly complete the operation of taking tips from the Tip storage area 181, and then control the pipetting robot to move in the XYZ axes to transfer the samples and reagents to the wells of the reaction plate. The rotating plate mechanical arm 120 is located on the right side of the pipetting mechanical arm 110, and can also respectively realize the control in the X direction, the Y direction and the Z direction, and the main actions are as follows: the PCR plate is transferred from the surface of the worktable 130 to the circulating temperature control module 210 through the circulating temperature control inlet 150, the ELISA plate is transferred from the surface of the worktable 130 to the detection module 220 through the detection inlet 160, or the experimental consumables such as the deep hole plate, the PCR plate, the ELISA plate and the like are mutually transferred on the surface of the worktable 130.

The bin dividing module is arranged below the workbench 130, and as shown in fig. 4, comprises a circulating temperature control module 210 and a detection module 220, the circulating temperature control module 210 comprises a temperature control sealing partition plate 211, a temperature control member and a temperature control sealing bin door, the temperature control member is installed in a temperature control closed cavity formed by the temperature control sealing partition plate 211, and the temperature control sealing bin door is arranged at a position matched with the circulating temperature control inlet 150; the detection module 220 comprises a detection sealing partition plate, a detection element and a detection sealing bin door 221, wherein the detection element is installed in a detection sealing cavity formed by the detection sealing partition plate, and the detection sealing bin door 221 is arranged at a position matched with the detection inlet 160.

It is understood that the temperature control member can be selected from a conventional thermal cycler (ODTC), the detection element can be selected from a microplate reader, and the like, and can be arranged according to the specific nucleic acid detection requirements.

Specifically, the temperature-controlled closed cavity and the detection closed cavity can utilize the separation between the partition board and each module, or the partition board forms a closed space. Through the above-mentioned independent branch storehouse space design, collocation efficient filters air draft system and ultraviolet lamp system, effectively prevents the air cross talk each other between the three, can fully carry out various molecular experiments according to PCR laboratory subregion requirement.

The technical personnel in the field know that when the ODTC starts, the interior of the ODTC needs to exchange heat to complete the temperature rise and decrease circulation, at the moment, the heat is completely dissipated into a temperature control sealed cavity formed by the temperature control sealed partition plate 211, and when the temperature in the sealed cavity rises to the low point 50 ℃ of the thermal circulation temperature of the ODTC, the ODTC is difficult to or cannot reach 50 ℃, and the experimental result is influenced. At this point, the exhaust system is started, and the ODTC exhaust system can set the rotation speed of the fan 410 to match the experiment requirements. For example, a relatively low rotation speed of about 1500rpm may be selected, and the setting may be made according to actual conditions, depending on the air volume of the fan 410. Accordingly, heat generated by ODTC thermal cycle is taken away by the air exhaust system, and the temperature rise and fall efficiency of ODTC can be effectively improved, so that the thermal cycle time is reduced, and the preparation efficiency of the whole library is further improved.

More importantly, after the ODTC process ends, a large amount of aerosol is generated at the instant the ODTC opens the thermal cap. At the moment, the air draft system is started, the aerosol generated instantly when the ODTC is opened can be taken away by the air draft system, the accumulation and sedimentation of the aerosol are effectively avoided, and the gene sequencing result is more reliable.

Therefore, the circulating temperature control module 210 of the embodiment further comprises a temperature control air draft system 213, wherein the temperature control air draft system 213 is arranged on the temperature control sealing partition plate 211 and is used for exhausting and filtering the air in the temperature control sealed cavity.

The fan used for heat discharge in the air draft system can be set to a relatively low rotating speed, such as about 1500rpm, and can be set according to actual conditions according to the air volume of different fans 410. The fan can be set at a relatively high rotation speed, such as about 3500rpm, when the fan is used for preventing aerosol pollution.

In this embodiment, the circulating temperature control module 210 further includes a cleaning cover 214, and the cleaning cover 214 is fixed on the temperature control sealing partition 211 and is opened in the horizontal direction. When the experiment is completed, the user can manually open the cleaning cap 214 to open the thermal cap of the ODTC, spray alcohol or other solvent associated with the inhibitory nucleic acid product onto the thermal cap, and wipe the thermal cap with a piece of dust-free paper to remove the aerosol product from the ODTC thermal cap.

In this embodiment, as shown in fig. 5 to 7, in order to achieve a better partition sealing effect in a smaller space region, an electric sealing door with the following structure is adopted, that is, the temperature-controlled sealing door and the detection sealing door 221 both include: mounting bracket 231, slide rail 232, slider 233, first connecting rod 2341, second connecting rod 2342, elastic component 235, driving motor 236, door panel 237, sealing member 238, push pedal 2392, lead screw and nut 2391, mounting bracket 231 is fixed in accuse temperature sealed baffle 211 or detect on the sealed baffle, slide rail 232 and driving motor 236 install in mounting bracket 231, slider 233 can follow slide rail 232 slides, door panel 237 can be in driving motor 236 pulls down along slide rail 232 direction removal to have closed state position and open state position.

Specifically, the lead screw with the driving motor 236 output is connected, nut 2391 cover is located on the lead screw, first connecting rod 2341 is through push pedal 2392 connects nut 2391. By means of the matching of the push plate 2392, the lead screw and the nut 2391, the stability and reliability of forward pushing and closing of the bin door can be improved. The first connecting rod 2341 is fixed on the slider 233, the bin door plate 237 is hinged and fixed to the first connecting rod 2341 through at least two second connecting rods 2342, the elastic member 235 (in this embodiment, a tension spring) is arranged between the first connecting rod 2341 and the bin door plate 237, when the bin door plate 237 is located in the open state position, under the elastic action of the elastic member 235, the bin door plate 237, the first connecting rod 2341 and the two second connecting rods 2342 form an inclined parallelogram, the bin door plate 237 is provided with a limiting wheel 2343, the mounting frame 231 is provided with a limiting member 2344, when the bin door plate 237 is located in the closed state position, the limiting wheel 2343 abuts against the limiting member 2344, the bin door plate 237 overcomes the elastic force of the elastic member 235 to move upwards, the first connecting rod 2341 and the two second connecting rods 2342 form a rectangle, the sealing member 238 is arranged on the upper surface of the bin door plate 237, and when the bin door plate 237 is located in the closed state, the lower surface of the sealing member 238 is extruded on the workbench 130, and is arranged around the circulating temperature control inlet 150 or the detection inlet 160.

The working principle of the temperature control sealing bin gate and the detection sealing bin gate 221 is as follows: when the bin gate needs to be closed, the driving motor 236 is started to rotate, under the driving of the lead screw and the nut 2391, the push plate 2392 moves forwards (moves leftwards in fig. 6-7) along the guide rail 143, when the limiting wheel 2343 moves to touch the limiting part 2344, the driving motor 236 overcomes the elastic force of the elastic part 235 (a tension spring) to change the parallelogram formed by the first connecting rod 2341, the second connecting rod 2342 and the bin gate plate 237 into a rectangle, so that the bin gate plate 237 is lifted in the height direction (Z axis), and the sealing part 238 is in close contact with the lower surface of the workbench 130, so that the sealing effect is achieved. When the door needs to be opened, the driving motor 236 is started to rotate reversely, and under the action of reverse thrust and elastic force, the rectangular structure is restored to be a parallelogram structure, so that the door opening action is completed. The two actions of pushing forward and closing the bin gate are completed under the control of a motor.

Furthermore, a bearing fixing part can be additionally arranged at the end part of the screw rod of the driving motor 236, so that the screw rod can be effectively prevented from being deformed due to overlong screw rod, and the movement is prevented from being blocked.

Preferably, in order to achieve a compact electrically controllable door, the driving motor 236 of the door is preferably a 28-type screw motor, and the structural thickness of the whole door can be reduced within a range of 34mm on the premise of ensuring the thrust.

For discernment door state, the sealed door of accuse temperature still includes light couple to 241 and opto-coupler separation blade 242 with detecting sealed door 221, light couple is located to 241 on the mounting bracket 231, opto-coupler separation blade 242 is located on push pedal 2392, storehouse door plant 237 is followed slide rail 232 direction removal has closed state position and open state position, works as storehouse door plant 237 is located closed state position, opto-coupler separation blade 242 is kept away from light couple is to 241, works as storehouse door plant 237 is located open state position, opto-coupler separation blade 242 shelters from light couple is to 241. The state of the bin gate can be identified by the on and off of the light couple to 241 signal. Fig. 6-7 illustrate the position where the limiting wheel 2343 is close to the limiting member 2344 but not yet abutting against the limiting member 2344, i.e., the cartridge door plate 237 is not yet closed and sealed.

It can be understood that, for the oscillation incubation system, the oscillation incubation system can be disposed on the right side of the Tip storage area 181 on the surface of the working platform 130 according to the conventional arrangement, and the basic incubation requirement can be satisfied. In this embodiment, a magnetic adsorption system 140 is further disposed beside the oscillation incubation region, and cooperates with the oscillation incubation region to form a nucleic acid extraction region, so as to heat and oscillate a sample, increase the nucleic acid cracking speed of the sample, and sufficiently mix magnetic beads, thereby shortening the experimental process time, preferably, the temperature range of the heating system is set at 0-80 ℃, and the oscillation speed of the oscillation system is set at 1400rpm/s (oscillation frequency 25Hz, amplitude 3 mm).

For further, in this embodiment, as shown in fig. 8, the magnetic force adsorption system 140 includes a magnetic rod 141, a connecting frame 142, a guide rail 143, and a motor 144, the magnetic rod 141 is fixed on the connecting frame 142, the connecting frame 142 is slidably connected to the guide rail 143 through the slider 233, and the connecting frame 142 is driven by the motor 144 to move up and down along the guide rail 143 and drive the magnetic rod 141 to move up and down.

When nucleic acid extraction is needed, the deep hole plate containing the cracked sample can be placed above the magnetic adsorption system 140, then the motor 144 is started, the magnetic rod 141 below is made to ascend and descend along the guide rail 143 and the slide block 233 through the connecting frame 142, the deep hole plate repeatedly moves up and down around the hole position, the liquid level in the deep hole plate and the bottom can be fully attracted by the magnetic rod 141, the adsorption rate of the magnetic beads is higher, for the sample with low concentration, the detection probability is higher, and the sensitivity of the reagent detection rate is improved.

And the magnetic rod 141 is controlled to move up and down, the height of the magnetic beads adsorbed on the wall of the deep hole plate can be controlled, when waste liquid in the hole needs to be removed (only pure magnetic beads adsorbed with nucleic acid are left after the reagent is sucked away), the magnetic beads are moved to a position 2mm away from the bottom surface of the deep hole plate, at this time, the liquid transferring mechanical arm 110 is moved to the position above the hole where the waste liquid needs to be removed, the gun tip of the liquid transferring pump with TIPS is moved down to a position 0.1-0.2 mm higher than the bottom of the deep hole plate, the waste liquid can be accurately sucked away without the magnetic beads, after the waste liquid is removed, eluent is added, the magnetic rod 141 is reset, the magnetic beads with the nucleic acid are separated in the eluent, the magnetic rod 141 is lifted and moved up and down by the magnetic frame 141, the magnetic beads free in the reagent are adsorbed on the side wall, the liquid transferring mechanical arm 110 is moved to the position above the hole where the liquid with the nucleic acid needs to be removed, the nucleic acid is sucked into the PCR reaction tube, and thus, the extraction of the nucleic acid is completed under the condition that the deep hole plate does not need to be transferred.

As shown in fig. 9, the waste module includes a garbage partition plate 310, a garbage can 320 and a garbage passage, the garbage can 320 is disposed in a garbage sealed cavity formed by the garbage partition plate 310 below the bin dividing module, one end of the garbage passage is open to the garbage can 320, and the other end of the garbage passage is open to the waste area 170 of the working platform 130.

In the conventional nucleic acid detection equipment, a Tips collection box is usually placed on a table, however, when the kit is used for detection of mNGS and the like, because the library building process is very long, more than 800 Tips are needed for statistics, and when the kit is placed on the table, at least more than 6 plate positions of space are needed for collecting the waste Tips, in order to solve the problem, a large garbage can 320 is placed at the bottom of the equipment to collect the waste Tips, so that the space of the table is not occupied, and the problem that the waste Tips are easy to cause aerosol pollution on the table is solved.

However, a new problem exists, the garbage passage leading from the working table to the bottom garbage can 320 is easily polluted by Tips in the falling process (a mixture of reagents and nucleic acids often remains in Tips, and when the Tips fall off, the free-falling body movement residues risk to be scattered around the passage).

Therefore, in this embodiment, the waste tips need to be thrown away after the pipetting robot 110 completes the experiment, and then the waste tips can enter the trash can 320 of the third layer of waste module from the surface of the platform 130 through the trash channel, the trash can 320 is disposed in the trash sealed cavity formed by the trash partition plate 310, one end of the trash channel is open to the trash can 320, and the other end of the trash channel is open to the waste area 170 of the platform 130.

However, due to the bin-dividing design, the first layer of the platform surface layer of the full-automatic sample library preparation system is the operation module layer, the second layer is the bin-dividing module layer, the third layer is the waste module layer, and the garbage channel penetrates through the three layers, so that the garbage channel is long inevitably, the channel is difficult to draw out manually, and in order to solve the problem, the garbage channel is designed in a sectional mode.

Specifically, a partition plate is arranged between the warehouse dividing module and the waste module, the garbage channel comprises an upper channel 331 and a lower channel 332 which are detachably sleeved and connected, the upper channel 331 is fixed on the table top of the workbench 130 through an upper flange 3311, and the lower channel 332 is fixed on the partition plate through a lower flange 3321, as shown in fig. 10.

That is, the upper channel 331 is inserted into the lower channel 332 in the space of the second layer, the size of the outer wall of the upper channel 331 is smaller than the size of the inner wall of the lower channel 332, the upper channel 331 has a flange edge, so that a user can directly insert into the waste Tips channel hole of the table top, the upper flange 3311 is arranged in the middle of the Tips channel two, so that the user can directly insert into the channel hole formed in the waste material area 170 on the table top 130 of the table top, when the user needs to take off the upper channel 331 and the lower channel 332, the upper channel 331 is lifted up until the bottom edge of the upper channel 331 is higher than the table top of the table 130, the upper channel 331 can be removed in a translation manner, and after the upper channel 331 is removed, the lower channel 332 is lifted up until the edge of the lower channel 332 is higher than the table top of the partition layer of the second layer, so as to remove the lower channel 332 in a translation manner. The two upper and

lower lanes

331, 332 are placed in the reverse order of removal.

The waste module further comprises a waste air draft system 340, wherein the air draft system is arranged on the garbage partition plate 310 and used for exhausting and filtering the air in the garbage closed cavity.

The main exhaust module 400 includes a fan 410 and a filter system 420, the main exhaust module 400 is installed on the top base plate 430 of the top of the operation module, 2 exhaust fans 410 are provided, and the air of the working table 130 passes through the filter system 420 (Hepa system) and is exhausted by the fan 410, as shown in fig. 11.

When the full-automatic sample library preparation system needs filtering maintenance, the equipment door is closed in a sliding mode, the main air draft module 400 is opened, the fan 410 conducts air draft outwards, the rotating speed of the fan 410 is about 3500RPM, the cavity of the operation module forms an air passage from bottom to top at the moment, negative pressure is formed in the inner cavity when the air draft module is drawn to a certain degree, the space is in a slight vacuum state, the air tends to flow from high pressure to low pressure according to the principle that the air flows from high pressure to low pressure, the air in the operation space cannot be blown around at will, the air is guided to flow in the direction far away from a clean area by the main air draft module 400 at the moment, as shown in fig. 12, the surface of the adsorption space is disinfected by matching with an ultraviolet disinfection lamp 510, and the air is discharged out of the space after being filtered by a Hepa system with the filtering efficiency of 99.999%, and therefore the excellent filtering effect is achieved.

It will be appreciated that the main extraction module 400, the temperature controlled extraction system 213 and the waste extraction system 340 are all exhausted from different extraction filter exhaust systems, as shown in figure 13, to avoid the possibility of cross contamination.

The control system comprises a main control board which is in signal connection or electric connection with the operation module and the warehouse separating module to control the operation module and the warehouse separating module.

In the fully automatic sample library preparation system, the liquid transfer and the movement of the pore plates (including pore plates used for tests such as deep pore plates, PCR plates, ELISA plates and the like) can be completed by the operation of the liquid transfer mechanical arm 110 and the plate rotating mechanical arm 120 of the operation module on the workbench 130, and the corresponding procedures of the samples are completed according to the requirements of nucleic acid detection procedures. And the independent compartment-separated treatment of the circulating temperature control module 210 and the detection module 220 can be realized through the opening and closing of the temperature control sealed compartment door and the detection sealed compartment door 221, so that the cross contamination risk is effectively reduced.

Meanwhile, in the full-automatic sample library preparation system, the inlets of the circulating temperature control module 210 and the detection module 220 are arranged below the workbench 130, and the circulating temperature control inlet 150 and the detection inlet 160 are arranged on the workbench 130, so that the working mode of the warehouse-dividing sealing treatment is realized.

In consideration of the requirement of the equipment for disinfection, an ultraviolet disinfection lamp 510 is arranged in the top of the operation module, the temperature control closed cavity and/or the garbage closed cavity.

When the operation module, the circulating temperature control module 210 or the waste module needs cleaning and maintenance, the ODTC ultraviolet lamp is arranged above the inside of the independent space, and the ultraviolet lamp can be turned on (the irradiation time of the ultraviolet lamp can be set, and intelligent timing can be realized), so that microorganisms in the space are sterilized, and a clean air system is effectively formed in the space. Preferably, the ultraviolet lamp is selected to have an irradiation dose of more than 100 000. Mu.W.s/cm 2.

Example 2

A library construction method adopts the full-automatic sample library preparation system and comprises the following steps:

the sample to be detected is placed on the workbench, the liquid transfer mechanical arm is controlled to absorb sample solution and reagent, the rotating plate mechanical arm is controlled to transfer the pore plate to a required position, and meanwhile, the opening and closing of the temperature control sealing bin door and the detection sealing bin door are controlled to be matched with the transfer of the rotating plate mechanical arm, so that the automatic operation of sample nucleic acid extraction, fragmentation, joint connection, library amplification and library purification is carried out.

That is, the full-automatic sample library preparation system of embodiment 1 can realize the whole process of library preparation such as nucleic acid purification, RNA fragmentation, cDNA one-strand synthesis (RNA reverse transcription), cDNA two-strand synthesis, dsDNA purification, DNA fragmentation, linker ligation, purification after ligation, library amplification, amplification product purification, library quality inspection, and the like.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is specific and detailed, but not to be understood as limiting the scope of the invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. A fully automated sample library preparation system, comprising:

the waste module comprises a waste partition plate, a waste bin and a waste channel, the waste bin is arranged in a waste closed cavity formed by the waste partition plate below the bin dividing module, one end of the waste channel is opened in the waste bin, and the other end of the waste channel is opened in a waste area of the workbench;

2. The full-automatic sample library preparation system according to claim 1, wherein the circulating temperature control module further comprises a temperature control air draft system, and the air draft system is arranged on the temperature control sealing partition plate and used for air draft filtration of the gas in the temperature control sealing cavity.

3. The full-automatic sample library preparation system of claim 1, wherein the waste module further comprises a waste air draft system, and the air draft system is arranged on the garbage partition plate and used for air draft filtration of the gas in the garbage closed cavity.

4. The fully automated sample library preparation system of claim 1, wherein the temperature-controlled sealed door and the detection sealed door each comprise: the device comprises a mounting frame, a sliding rail, a sliding block, a first connecting rod, a second connecting rod, an elastic part, a driving motor, a bin door panel and a sealing part, wherein the mounting frame is fixed on the temperature control sealing partition plate or the detection sealing partition plate; the first connecting rod is fixed in on the slider, the storehouse door plant through two at least second connecting rods with first connecting rod is articulated fixed, the elastic component is located first connecting rod or second connecting rod with between the door plant, work as the storehouse door plant is located open state position under the elastic component spring action, the storehouse door plant constitutes the parallelogram of slope with first connecting rod and two second connecting rods, be equipped with spacing wheel on the door plant of storehouse, be equipped with the locating part on the mounting bracket, work as the storehouse door plant is located close state position, spacing wheel support lean on in the locating part, the storehouse door plant overcomes the elasticity of elastic component moves up, constitutes the rectangle with first connecting rod and two second connecting rods, the sealing member is located storehouse door plant upper surface, work as the storehouse door plant is located close state position, the sealing member be extruded in the workstation lower surface, and encircle circulation accuse temperature entry or detect the entry setting.

5. The system according to claim 4, wherein the temperature-controlled sealing door and the detection sealing door further comprise a push plate, a lead screw and a nut, the lead screw is connected with the output end of the driving motor, the nut is sleeved on the lead screw, and the first connecting rod is connected with the nut through the push plate.

6. The system according to claim 5, wherein the temperature-controlled and detection-sealed doors further comprise a light coupling pair and an optical coupling blocking piece, the optical coupling pair is disposed on the mounting frame, the optical coupling blocking piece is disposed on the pushing plate, the bin door panel has a closed state position and an open state position along the sliding rail guide movement, when the bin door panel is located in the closed state position, the optical coupling blocking piece is far away from the light coupling pair, and when the bin door panel is located in the open state position, the optical coupling blocking piece blocks the light coupling pair.

7. The fully automatic sample library preparation system of claim 1, wherein the circulating temperature control module is further provided with a cleaning cover, and the cleaning cover is fixed on the temperature control sealing partition plate and is opened in the horizontal direction.

8. The system according to claim 1, wherein an ultraviolet disinfection lamp is disposed in the top of the operation module, the temperature-controlled closed cavity and/or the garbage closed cavity.

9. The fully automated sample library preparation system of claim 1, wherein the platform comprises a refrigeration module zone and a cold zone, the refrigeration module zone being provided with refrigeration equipment to maintain the refrigeration module zone at a low temperature.

10. The system for preparing a full-automatic sample library according to claim 1, wherein the workbench is provided with a Tip storage area, a refrigeration module area, a normal temperature area, a vibration incubation area, a waste area and a consumable material placing area in sequence from one side to the opposite side.

11. The system according to claim 1, wherein the operation module further comprises a magnetic adsorption system, the magnetic adsorption system comprises a magnetic rod, a connecting frame, a guide rail and a motor, the magnetic rod is fixed on the connecting frame, the connecting frame is slidably connected with the guide rail through the sliding block, and the connecting frame is driven by the motor to move up and down along the guide rail and drive the magnetic rod to move up and down.

12. The system of claim 1, wherein a spacer plate is disposed between the bin dividing module and the waste module, the waste channel comprises an upper channel and a lower channel detachably sleeved and connected with each other, the upper channel is fixed on the table top of the workbench through an upper flange, and the lower channel is fixed on the spacer plate through a lower flange.

13. The fully automatic sample library preparation system of claim 1, further comprising a main air draft module, wherein the main air draft module comprises a fan and a filtering system, the main air draft module is installed on the top of the operation module, and the air of the workbench is exhausted through the filtering system by the fan.

14. Use of the fully automated sample library preparation system of any one of claims 1-13 in nucleic acid detection library construction.

15. A method for library construction, wherein the fully automated sample library preparation system of any one of claims 1 to 13 is used, comprising the steps of: the sample to be detected is placed on the workbench, the liquid transfer mechanical arm is controlled to absorb sample solution and reagent, the rotating plate mechanical arm is controlled to transfer the pore plate to a required position, and meanwhile, the opening and closing of the temperature control sealing bin door and the detection sealing bin door are controlled to be matched with the transfer of the rotating plate mechanical arm, so that the automatic operation of sample nucleic acid extraction, fragmentation, joint connection, library amplification and library purification is carried out.