CN212504894U - Gene detecting apparatus - Google Patents

Gene detecting apparatus Download PDF

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Publication number
CN212504894U
CN212504894U CN202022322757.6U CN202022322757U CN212504894U CN 212504894 U CN212504894 U CN 212504894U CN 202022322757 U CN202022322757 U CN 202022322757U CN 212504894 U CN212504894 U CN 212504894U
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module
assembly
installation chamber
gene
logistics transportation
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陈健
周德江
李�杰
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Chengdu Hanchen Guangyi Bioengineering Co ltd
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Chengdu Hanchen Guangyi Bioengineering Co ltd
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Abstract

The utility model discloses a gene detection device, which relates to the gene detection field, comprising a safety cabinet, an air flow control system, a first processing module for processing an amplification reagent and a PCR plate, a second processing module for processing the reagent, a gun head and a gene sample, a sample adding module for adding the amplification reagent into a micropore of the PCR plate, a purification processing module for processing the gene sample, an amplification detection module for gene amplification and detection, a heat sealing machine for sealing a film on the PCR plate, a logistics transportation module A, a logistics transportation module B and a logistics transportation module C; through the utility model discloses a gene detection equipment reaches automatic pollution-free efficient gene detection, and simultaneously in the testing process, only a small part involves manual operation, and manual operation intensity is low.

Description

Gene detecting apparatus
Technical Field
The utility model relates to a gene detection field especially relates to a gene detection equipment.
Background
The gene detection refers to the analysis of the base sequence of a specific DNA fragment, namely the arrangement mode of adenine, thymine, cytosine and guanine; the gene is a genetic basic unit, carries DNA or RNA sequence of genetic information, transmits the genetic information to the next generation through replication, and guides the synthesis of protein to express the genetic information carried by the gene, thereby controlling the character expression of organism individuals. The gene detection is a technique for detecting DNA by blood, other body fluids or cells, and is a method for taking peripheral venous blood or other tissue cells of a detected person, amplifying the gene information, detecting DNA molecular information in the cells of the detected person by a specific device, and analyzing whether the gene type, the gene defect and the expression function contained in the DNA molecular information are normal or not, so that people can know the gene information of themselves, determine the cause of disease or predict the risk of the body suffering from a certain disease, and the gene detection can diagnose the disease and can also be used for predicting the risk of the disease.
The gene detection equipment in the prior art cannot realize automation of the whole detection process, so that the gene detection efficiency is low.
Therefore, it is urgently required to develop a gene testing apparatus to solve the above problems.
SUMMERY OF THE UTILITY MODEL
The object of the present invention is to provide a gene testing device for solving the above problems.
The utility model discloses a following technical scheme realizes above-mentioned purpose:
a genetic testing device comprising:
a safety cabinet; the safety cabinet is divided into an upper layer and a lower layer which are relatively isolated, each layer of the safety cabinet is divided into three relatively isolated installation chambers, the installation chambers on the upper layer are respectively a first installation chamber A, a first installation chamber B and a first installation chamber C, and the installation chambers on the lower layer are respectively a second installation chamber A, a second installation chamber B and a second installation chamber C; a first switching door assembly A is arranged between the first installation chamber A and the second installation chamber A, a first switching door assembly B is arranged between the first installation chamber B and the second installation chamber B, a first switching door assembly C is arranged between the first installation chamber C and the second installation chamber C, a second switching door assembly A is arranged between the second installation chamber A and the second installation chamber B, and a second switching door assembly B is arranged between the second installation chamber B and the second installation chamber C;
an airflow control system; the air flow control system is used for controlling the air pressure conditions of the first installation chamber A, the first installation chamber B and the first installation chamber C;
a first processing module for processing amplification reagents and the PCR plate;
the second processing module is used for processing the reagent, the gun head and the gene sample;
the sample adding module is used for adding an amplification reagent into the micropores of the PCR plate;
the purification processing module is used for processing the gene sample;
an amplification detection module for gene amplification and detection;
a heat sealer for sealing the PCR plate;
a logistics transportation module A;
a logistics transportation module B;
a logistics transportation module C;
the sample adding module and the first processing module are installed in a first installation chamber A, the purifying processing module, the second processing module and the heat sealing machine are installed in a first installation chamber B, the amplification detection module is installed in a first installation chamber C, and the logistics transportation module A, the logistics transportation module B and the logistics transportation module C are installed in the second installation chamber A, the second installation chamber B and the second installation chamber C respectively; the logistics transportation module A, the logistics transportation module B and the logistics transportation module C are respectively positioned below the sample adding module, the purification processing module and the amplification detection module; during transportation, the PCR plate with the amplification reagent passes through the first switch door assembly A and is arranged at the transfer action end of the logistics transportation module A through the sample adding module, when the logistics transportation module A transfers the PCR plate to the second switch door assembly A, the PCR plate is arranged at the transfer action end of the logistics transportation module B, when the logistics transportation module B transfers the PCR plate to the first switch door assembly B, the PCR plate enters the first installation chamber B through the purification treatment module, the purified gene sample is added into the PCR plate, after the heat sealing machine carries out heat sealing, the PCR plate with the gene sample is arranged at the transfer action end of the logistics transportation module B through the first switch door assembly B of the purification treatment module, when the logistics transportation module B transfers the PCR plate to the second switch door assembly B, the PCR plate is arranged at the transfer action end of the logistics transportation module C, when the logistics transportation module C transfers the PCR plate to the first switch door assembly C, and (3) entering the first installation chamber C through the purification processing module, and carrying out gene detection on the gene sample in the PCR plate by the amplification detection module.
The beneficial effects of the utility model reside in that: through the utility model discloses a gene detection equipment reaches automatic pollution-free efficient gene detection, and simultaneously in the testing process, only a small part involves manual operation, and manual operation intensity is low.
Drawings
Fig. 1 is a schematic perspective view of the present invention;
fig. 2 is a schematic perspective view of the sample adding module of the present invention;
fig. 3 is a schematic structural diagram 1 of a second processing module according to the present invention;
fig. 4 is a schematic structural diagram of a second processing module of the present invention, fig. 2;
fig. 5 is a schematic structural view of a heating module in the second processing module of the present invention;
fig. 6 is a schematic structural view of a rack D in a second process module according to the present invention;
fig. 7 is a schematic structural diagram of an opening and closing assembly in a second processing module according to the present invention;
FIG. 8 is a schematic illustration of the opening and closing assembly of the purification process module of the present invention;
FIG. 9 is a schematic diagram of the structure of the purification processing module of the present invention, FIG. 3;
fig. 10 is a schematic structural diagram 4 of a second processing module of the present invention;
FIG. 11 is a schematic diagram of a purification module of the purification processing module of the present invention;
FIG. 12 is a schematic view of the heat seal case of the present invention;
fig. 13 is a schematic structural view of an induced draft assembly in a second processing module according to the present invention;
FIG. 14 is a schematic structural view of a transfer element B in the purification treatment module of the present invention;
FIG. 15 is a schematic view of the use of a transfer element B in the purification process module of the present invention;
FIG. 16 is a schematic diagram of the amplification and detection module of the present invention;
FIG. 17 is a schematic structural view of a translation module C of the amplification detection module of the present invention;
fig. 18 is a schematic structural diagram of a logistics transportation module a and a logistics transportation module B of the present invention;
fig. 19 is a schematic view of the logistics transportation module a and the logistics transportation module B of the present invention during transportation;
fig. 20 is a schematic view of the logistics transportation module B and the logistics transportation module C of the present invention before transportation;
fig. 21 is a schematic diagram of the logistics transportation module B and the logistics transportation module C after transportation according to the present invention;
fig. 22 is a schematic view of the airflow direction of the airflow control system of the present invention;
wherein corresponding reference numerals are:
1-a first installation chamber A, 2-a first installation chamber B, 3-a first installation chamber C, 4-a second installation chamber A, 5-a second installation chamber B, 6-a second installation chamber C, 7-a first switching door assembly A, 8-a first switching door assembly B, 9-a first switching door assembly C, 10-a second switching door assembly A, 11-a second switching door assembly B, 18-a high-efficiency filter A, 19-an induced draft fan A, 22-a bracket, 23-a lifting assembly B, 24-a translation assembly E, 26-a code scanner, 29-a liquid transfer assembly B and 30-a translation assembly I;
12-transfer assembly, 1201-X movement assembly, 1202-Y movement assembly, 1203-Z movement assembly, 1204-pipette a, 1205-gripper C;
13-a first treatment module, 1301-a rack a, 1302-a rack B, 1303-a cooling module a;
14-a second processing module, 1401-a rack C, 1402-a rack D, 1403-a rack E, 1404-a rack F, 1405-a temporary rack, 1406-a heating module, 1408-an air inducing component, 1409-a spring plate, 1410-a placement groove, 1411-a middle indexing part, 1412-a vibrating part A;
15-amplification detection module, 1501-optical detector, 1502-heating base, 1503-heat cover component, 1504-translation component C;
16-jaw a, 1601-jaw control a;
17-purification module, 170-1 thermal oscillation chamber, 1702-magnetic attraction piece, 1703-translation component A, 1704-reaction mounting frame, 1705-vibration piece B;
20-an opening and closing assembly, 2001-a lifting assembly A, 2002-a clamping jaw B, 2003-a fixed block A2004-a fixed block B, 2005-a translation assembly B, 2006-a clamping ring, 2007-a high-friction cushion;
21-translation assembly D, 2101-motor D, 2102-driving wheel D, 2103-driven wheel D, 2104-driving belt D, 2105-mounting plate D, 2106-sliding rail;
25-solid-liquid waste discharge piece, 2501-translation assembly G, 2502-cover plate;
28-heat seal enclosure, 2801 third switch assembly, 2802 heat sealer, 2803 mount plate F, 2804 translation assembly H;
31-a liquid receiving assembly; 3101 the liquid receiving plate, 3102 the liquid receiving groove, 3103 the mounting shaft, 3104 the liquid receiving frame, 3105 the groove, 3106 the boss, and 3107 the torsion spring.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "inner", "outer", "left", "right", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships that are conventionally placed when the products of the present invention are used, or orientations or positional relationships that are conventionally understood by those skilled in the art, and are merely for convenience of description of the present invention and simplifying the description, but do not indicate or imply that the device or element that is referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed" and "connected" are to be interpreted broadly, and for example, "connected" may be a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; the connection may be direct or indirect via an intermediate medium, and may be a communication between the two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
The following describes in detail embodiments of the present invention with reference to the accompanying drawings.
As shown in fig. 1, the gene assaying device comprises:
a safety cabinet; the safety cabinet is divided into an upper layer and a lower layer which are relatively isolated, each layer of the safety cabinet is divided into three relatively isolated installation chambers, the installation chambers on the upper layer are respectively a first installation chamber A1, a first installation chamber B2 and a first installation chamber C3, and the installation chambers on the lower layer are respectively a second installation chamber A4, a second installation chamber B5 and a second installation chamber C6; a first switch door assembly A7 is arranged between the first installation chamber A1 and the second installation chamber A4, a first switch door assembly B8 is arranged between the first installation chamber B2 and the second installation chamber B5, a first switch door assembly C9 is arranged between the first installation chamber C3 and the second installation chamber C6, a second switch door assembly A10 is arranged between the second installation chamber A4 and the second installation chamber B5, and a second switch door assembly B11 is arranged between the second installation chamber B5 and the second installation chamber C6;
an airflow control system; the air flow control system is used for controlling the air pressure of the first mounting chamber A1, the first mounting chamber B2 and the first mounting chamber C3;
a first processing module 13 for processing amplification reagents and PCR plates;
a second processing module 14 for processing the reagent, the tip and the gene sample;
the sample adding module is used for adding an amplification reagent into the micropores of the PCR plate;
the purification processing module is used for processing the gene sample;
an amplification detection module 15 for gene amplification and detection;
a heat sealer 2802 for sealing the PCR plate;
a logistics transportation module A;
a logistics transportation module B;
a logistics transportation module C;
wherein the sample adding module and the first processing module 13 are installed in a first installation chamber A1, the purification processing module, the second processing module 14 and the heat sealing machine are installed in a first installation chamber B2, the amplification detection module 15 is installed in a first installation chamber C3, and the logistics transportation module A, the logistics transportation module B and the logistics transportation module C are respectively installed in a second installation chamber A4, a second installation chamber B5 and a second installation chamber C6; the logistics transportation module A, the logistics transportation module B and the logistics transportation module C are respectively positioned below the sample adding module, the purification processing module and the amplification detection module 15; during transportation, the PCR plate with the amplification reagent passes through the first switch door assembly A7 and is arranged at the transferring action end of the logistics transportation module A through the sample adding module, when the logistics transportation module A transfers the PCR plate to the second switch door assembly A10, the PCR plate is arranged at the transferring action end of the logistics transportation module B, when the logistics transportation module B transfers the PCR plate to the first switch door assembly B8, the PCR plate enters the first installation chamber B2 through the purification treatment module, the purified gene sample is added into the PCR plate, after the heat sealing machine 2802 is subjected to heat sealing, the PCR plate with the gene sample is arranged at the transferring action end of the logistics transportation module B through the first switch door assembly B8 of the purification treatment module, when the logistics transportation module B transfers the PCR plate to the second switch door assembly B11, the PCR plate is arranged at the transferring action end of the logistics transportation module C, when the logistics transportation module C transfers the PCR plate to the first switch door assembly C9, the amplification detection module 15 performs gene detection on the gene sample in the PCR plate by passing the purification processing module into the first installation chamber C3.
As shown in fig. 1, fig. 2, fig. 3, fig. 4, and fig. 9, each of the loading module, the purification processing module, and the amplification detection module includes a transfer assembly 12, a clamping jaw a16 for transferring a PCR plate, and a clamping jaw control a1601 for controlling the opening and closing of the clamping jaw a16, wherein the loading module and the purification processing module further include a pipetting member a1204 for transferring liquid, and each transfer assembly 12 includes:
an X-direction moving component 1201 for X-direction movement; the X-direction moving assembly comprises a motor A, a driving wheel A, a driven wheel A, a transmission belt A and a mounting plate A, wherein the mounting plate A is fixedly connected with the transmission belt A, a rotating shaft of the motor A is fixedly connected with a rotating center of the driving wheel A, and the driving wheel A and the driven wheel A are transmitted through the transmission belt A;
a Y-direction moving assembly 1202 for Y-direction movement; the Y-direction moving assembly comprises a motor B, a driving wheel B, a driven wheel B, a transmission belt B and a mounting plate B, the mounting plate B is fixedly connected with the transmission belt B, a rotating shaft of the motor B is fixedly connected with a rotating center of the driving wheel B, and the driving wheel B and the driven wheel B are transmitted through the transmission belt B;
a Z-direction moving assembly 1203 for Z-direction movement; the Z-direction moving assembly comprises a motor C, a lead screw nut mechanism A and a mounting plate C, a rotating shaft of the motor C is fixedly connected with a lead screw A of the lead screw nut mechanism A, the mounting plate C is fixedly connected with a nut A of the lever nut mechanism A, and the clamping jaw control piece A1601 and the liquid-moving piece A1204 are fixedly mounted on the mounting plate C;
wherein, the X-direction moving assembly 1201 of the sample adding module and the amplification detection module is arranged on the mounting plate B, and the Z-direction moving assembly 1203 is fixedly arranged on the mounting plate A; purification process module Y-direction moving assembly 1202 is mounted on mounting plate a and Z-direction moving assembly 1203 is fixedly mounted on mounting plate B.
In the present embodiment, in the first installation chamber a 1: the sample adding module changes the position of the liquid transferring piece A1204 in three directions of XYZ through the transferring component 12, so that the amplification reagents are transferred to the micropores of the PCR plate, the clamping jaw control piece A1601 controls the clamping jaw A16 to open and close to grab the PCR plate, and the PCR is transferred to pass through the first opening and closing door component A7 and then placed at the transferring action end of the logistics transportation module A; in the first installation chamber B2: after the purification treatment module carries out purification treatment on the gene sample, the purification treatment module controls a PCR plate on the opening and closing grabbing logistics transportation module B transfer end of a clamping jaw A16 to pass through a first switch door assembly B8 to be transferred to a first installation chamber B2 through a clamping jaw control piece A1601, the lifted and treated gene sample is placed in a micropore of the PCR plate through a liquid transferring piece A1204, then the membrane sealing plate is sealed on the PCR plate filled with the gene sample through a heat sealing machine 2802, and finally the PCR plate filled with the gene sample is placed at the transfer end of the logistics transportation module B through a clamping jaw A16 and passes through a first switch door assembly B8; within the first mounting compartment C3: the amplification detection module 15 controls the PCR plate on the transfer action end of the opening and closing grasping logistics transportation module C of the clamping jaw A16 through the clamping jaw control piece A1601 to transfer into the first installation chamber C3 through the first opening and closing door assembly C9, and the amplification and optical detection are carried out on the gene sample through the amplification detection module 15.
As shown in fig. 2, the first processing module 13 includes at least two sets of racks a1301, B1302, and a cooling module a1303 for cooling amplification reagents, the test tubes with amplification reagents are placed on the rack a1301, the number of amplification reagents on one set of racks a1301 is not less than the number of amplification reagents required by a gene sample experiment, the PCR plate is placed on the rack B1302, an opening is provided on a side surface of the first installation chamber A1, both the rack a1301 and the rack B1302 can be placed in the first installation chamber A1 in a drawer-type manner, and the cooling module a1303 is installed right below the rack a 1301.
In this embodiment, the first processing module further includes a cleaning tank for cleaning the pipetting piece a1204, after the pipetting piece a1204 transfers the amplification reagent once, the transfer assembly 12 transfers the pipetting end of the pipetting piece a1204 into the cleaning tank, the cleaning tank cleans the pipetting end of the pipetting piece a1204, and then the next amplification reagent pipetting operation is performed, at least two PCR plates are placed on the placing rack B1302, and the arrangement of at least two sets of placing racks a1301 ensures that the sample adding process can be continuously performed, so that the influence of manual replenishment of the amplification reagent is avoided, and the efficiency of gene detection is improved.
As shown in fig. 1, 3, 4, 5, 6, 9 and 10, the second processing module 14 comprises at least two sets of racks C1401, at least two sets of racks D1402, E1403, at least two sets of temporary racks 1405, a rack F1404, a vibrating piece a1412 for vibrating a magnetic bead solution, a heating module 1406 for heating a lysate and a cooling module B for cooling a purified gene sample, wherein the purifying reagent, the eight connecting pipes for purification reaction and the replacement tips a are placed on the racks C1401, the number of the purifying reagent, the number of the eight connecting pipes and the number of the replacement tips a on one set of racks C1401 are respectively not less than the number of the purifying reagent, the number of the eight connecting pipes and the number of the replacement tips a required by one group of gene sample experiments, the sample pipes containing the gene sample are placed on the racks D1402, the number of the gene sample on one set of racks D1402 is not less than the number of the gene sample required by one group of gene sample experiments, a washing liquid for eluting a gene sample is placed in a placing rack E1403, a replacement gun head B and a membrane sealing plate are both placed in a placing rack F1404, a PCR plate which is not filled with the purified gene sample is placed on a temporary storage rack 1405, a cooling module B is installed right below the temporary storage rack 1405, the side surface of a first installation chamber B2 is provided with an opening, the placing rack C1401, the placing rack D1402, the placing rack E1403 and the placing rack F1404 are all placed in a first installation chamber B2 in a drawer type mode through the opening, the second processing module further comprises a transfer position 1411, and the transfer position 1411 is used for placing an eight-connected pipe which is not filled with the gene sample.
In this embodiment, the heating module 1406 is used for heating the lysate to prevent the lysate from crystallizing, the height of the first end of the heating module 1406 is lower than that of the second end of the heating module 1406, when the placing rack C1401 is inserted into the first installation chamber B2, the magnetic bead solution is vibrated by the vibrating member a1412 passing through the first end and the second end of the heating module 1406 in sequence, so as to keep the magnetic bead solution in a uniform state and prevent the magnetic bead solution from being deposited and affecting the purification reaction, and the arrangement of at least two sets of placing racks C1401, D1402, E1403, 1405 and F1404 ensures that corresponding spare parts are still left in the first installation chamber B2 during the replacement time of the purification reaction or the purification reaction is not substantially impressive during the replacement time of the manual replacement, so as to ensure that all processes of the purification reaction in the first installation chamber B2 can be performed uninterruptedly, thereby achieving the purpose of improving the gene detection efficiency.
As shown in fig. 6, the rack D1402 is provided with a plurality of placement grooves 1410 for placing the sample tubes, one side of the placement groove 1410 is provided with a spring piece 1409, the direction in which the spring piece 1409 recovers deformation is directed toward the central axis of the placement groove 1410, and when the sample tubes are placed in the placement grooves 1410, the spring piece 1409 is in pressing contact with the side walls of the sample tubes.
In this embodiment, the sample tube is inserted and placed in the placing groove 1410 of the placing rack D1402, the side wall of the sample tube is in pressing contact with the spring piece 1409, the purpose of fixing the sample tube is achieved, the sample tube is prevented from shaking in the moving process, a plurality of barcodes are arranged on the placing rack, one placing groove 1410 is located between two adjacent barcodes, the placing rack C1401 is also provided with a plurality of barcodes, a test tube filled with purifying reagents is located between two adjacent barcodes, the gene detection device further comprises two barcodes 26 used for scanning and identifying barcodes, the specific position and information of each sample tube and the purifying reagents are identified through the barcodes, and internal reference samples used for comparing whether the test result has errors or not are also placed on the placing rack D1402.
The gene detection equipment further comprises a plurality of sets of fixing devices, the fixing devices are respectively used for fixing the placing rack A1301, the placing rack B1302, the placing rack C1401, the placing rack D1402 and the placing rack E1403, each fixing device comprises an in-place sensor and an electromagnet, and when the in-place sensors detect that the corresponding placing racks are inserted in place, the electromagnets adsorb and fix the corresponding placing racks.
In this embodiment, when the placing rack a1301 and the placing rack B1302 are inserted into the first installation chamber A1, and the in-place sensor recognizes that the placing rack a1301 and the placing rack B1302 are inserted in place, the electromagnet is energized, and the placing rack a1301 and the placing rack B1302 are attracted and fixed by the electromagnet; when the placing rack C1401, the placing rack D1402 and the placing rack E1403 are inserted into the first installation chamber B2, the in-place sensor recognizes that the placing rack C1401, the placing rack D1402 and the placing rack E1403 are inserted in place, the electromagnet is electrified, and the placing rack C1401, the placing rack D1402 and the placing rack E1403 are fixedly adsorbed by the electromagnet.
As shown in fig. 9, 10 and 13, the second processing module further includes at least two sets of translation assemblies F and at least two sets of air inducing assemblies 1408 for absorbing the aerosol generated from the gene sample, wherein the translation assemblies F are used for changing the distance between the air inlets of the air inducing assemblies 1408 and the temporary storage rack 1405, and the air inlets of the air inducing assemblies 1408 are located directly above the temporary storage rack 1405 during air induction.
In this embodiment, the induced air subassembly includes high efficiency filter C and draught fan D, and high efficiency filter C's gas outlet and draught fan D's air inlet intercommunication, the PCR board that does not fill with the gene sample is kept in temporary storage and is placed on keeping in frame 1405, and translation subassembly F removes high efficiency filter D's air inlet and makes it be located the PCR board directly over, starts draught fan D, absorbs the aerosol that the gene sample produced, avoids aerosol to cause the pollution of first installation room B2.
As shown in fig. 9, 10, and 11, the purification processing module further includes at least one purification module 17, a high-efficiency filter a18, and an induced draft fan a19, each purification module 17 includes a heating oscillation chamber 1701, a magnetic attraction piece 1702, a vibration piece B1705, a translation component a1703, and a heating piece, the heating oscillation chamber 1701 is provided with a reaction mounting rack 1704, eight tubes for performing purification reactions are placed on the reaction mounting rack 1704, a heating action end of the heating piece acts on the eight tubes, the heating piece is installed at a vibration action end of the vibration piece B1705, the translation component a1703 is used for moving the magnetic attraction piece 1702 to change an attraction force of the magnetic attraction piece 1702 on the magnetic beads in the eight tubes, an air outlet is provided at a side of the heating oscillation chamber 1701, one of the air inlets of the high-efficiency filter a18 is communicated with an air outlet of the heating oscillation chamber 1701, and an air inlet of the.
In this embodiment, an eight-connected tube is placed on a reaction mounting frame 1704, a heating element and a vibrating element B1705 are started, a gene sample in the eight-connected tube is heated for purification treatment, the vibrating element B enables the gene sample and a purification reagent in the eight-connected tube to be uniformly mixed, in the purification reaction process, an induced draft fan a19 is started, aerosol generated in the purification reaction process passes through an air inlet of an efficient filter a18, the aerosol is filtered by the efficient filter a18, after the purification reaction, a translation component a1703 moves a magnetic attraction element 1702 to be close to the eight-connected tube, the magnetic attraction element 1702 adsorbs magnetic beads in the eight-connected tube, when the magnetic beads are combined with the gene sample, the gene sample is separated from waste liquid, and then the waste liquid is sucked out and discharged through a liquid transfer; when the magnetic beads are not combined with the gene sample, the gene sample is separated from the magnetic beads, so that the pipetting element A1204 can conveniently suck and transfer the gene sample into a PCR plate filled with amplification reagents.
As shown in fig. 3, 7 and 8, the purification processing module further includes an opening and closing assembly 20 for opening and closing the tube cap in the sample tube, the opening and closing assembly includes a lifting assembly a2001, a clamping jaw B2002, a clamping jaw control member B for controlling the opening and closing of the clamping jaw B2002, and a tube fixing member for fixing the tube body in the sample tube, the lifting action end of the lifting assembly a2001 acts on the clamping jaw control member B, and the tube fixing member is disposed below the clamping jaw B2002.
In this embodiment, the sample tube is fixed by the tube fixing member, the distance between the clamping jaws B2002 is increased by the clamping jaw control member B, the lifting assembly a2001 lowers the clamping jaw control member B and the clamping jaws B2002 to the tube cover of the sample tube, the clamping jaw control member B decreases the distance between the clamping jaws B2002, so that the clamping jaws B2002 clamp the tube cover, and then the lifting assembly a2001 raises the heights of the clamping jaw control member B and the clamping jaws B2002 to achieve the purpose of opening the cover, and the reverse is the cover closing operation flow.
As shown in fig. 3, 7 and 8, the tube fixing member includes a fixing block a2003, a fixing block B2004 and a translation assembly B2005 for controlling a distance between the fixing block a2003 and the fixing block B2004, the fixing block B2004 is installed right below the clamping jaw B2002, a moving action end of the translation assembly B2005 acts on the fixing block a2003, side surfaces of the fixing block a2003, which are opposite to the fixing block B2004, are both arc surfaces, and a clamping ring 2006 for clamping and hanging the sample tube is arranged at an upper end of the arc surface of the fixing block a 2003.
In this embodiment, the sample tube is taken out of the placement groove of the placement rack D1402 by the purification processing module, and then transferred into the snap ring 2006 of the fixed block a2003, and then the fixed block a2003 is moved by the translation assembly B2005, so that the side wall of the sample tube is respectively in pressing contact with the arc surfaces of the fixed block a2003 and the fixed block B2004, thereby achieving the purpose of fixing the sample tube, meanwhile, after the gene sample is taken out by opening the cover, the tube cover is closed by covering, the translation assembly B2005 moves the fixed block a2003 away from the fixed block B2004, and finally the sample tube is placed in the placement groove of the placement rack D1402 when the sample tube is taken out correspondingly by the purification processing module.
As shown in fig. 7, a high-friction cushion 2007 is fixedly provided on the arc surfaces of the fixed block a2003 and the fixed block B2004.
In the present embodiment, the fixing action of the sample tube is enhanced by fixedly providing the high-friction cushion 2007 on the arc surfaces of the fixed block a2003 and the fixed block B2004 so as to enhance the pressing force between the sample tube and the fixed blocks a2003 and B2004 when the sample tube is fixed.
As shown in fig. 1, 3, 4, 9 and 10, the purification treatment module further includes two sets of transfer assemblies 12, a clamping jaw C1205 for transferring sample tubes and eight-connected tubes, a clamping jaw control member C for controlling the clamping jaw C1205 to open and close, and two sets of pipetting members a1204 for transferring liquid, three pipetting members a1204 of the purification treatment module are respectively used for transferring purification reagents, cleaning solutions and gene samples, the pipetting member a1204 for transferring cleaning solutions and the clamping jaw control member a1601 are mounted on the mounting plate C of the same transfer assembly 12, the pipetting member a1204 for transferring gene samples and the clamping jaw control member C are mounted on the mounting plate C of the same transfer assembly 12, and the pipetting member a1204 for transferring purification reagents is mounted on the mounting plate C of another transfer assembly 12.
In the present embodiment, the purification processing module comprises three sets of transfer assemblies 12, a clamping jaw C1205 for transferring sample tubes and eight-connected tubes, a clamping jaw control C for controlling the clamping jaw C1205 to open and close, and three sets of pipettes a1204 for transferring liquid, wherein the three pipettes a1204 are used for transferring purification reagents, cleaning solutions, and gene samples before purification, respectively, and wherein the pipettes a1204 and the clamping jaw control a1601 for transferring cleaning solutions are mounted on the mounting plate C of the same transfer assembly 12; a liquid transferring piece A1204 and a clamping jaw control piece C for transferring a gene sample before purification are arranged on the mounting plate C of the same transfer assembly 12, and a liquid transferring piece A1204 for transferring a purification reagent is arranged on the mounting plate C of the other transfer assembly 12, so that the complementary interference of the three transfer assemblies 12 in the whole purification process is ensured, and simultaneously, the simultaneous performance of multiple groups of experiments can also be ensured, the time in the purification reaction process is shortened, and the detection efficiency is improved, wherein the liquid transferring piece A1204 for transferring the purification reagent is ADP30110869 of TECAN; the second processing module further comprises a cleaning pool, the cleaning pool is used for cleaning the pipetting piece A1204 for transferring the gene sample before purification, after the pipetting piece A1204 transfers the amplification reagent for one time, the transfer component 12 transfers the pipetting end of the pipetting piece A1204 into the cleaning pool, the cleaning pool cleans the pipetting end of the pipetting piece A1204, and then the next amplification reagent pipetting operation is carried out; the replacement tip A is used for replacing the pipette tip of the pipetting piece A1204 for transferring the purifying reagent, and the replacement tip B is used for replacing the pipette tip of the pipetting piece A1204 for transferring the cleaning solution.
As shown in fig. 14 and 15, the purification processing module further includes a pipetting device B29 for micropipette, a translation device I30, and a liquid receiving device 31 for receiving liquid from the pipetting device B29, the pipetting device B29 is used for transferring purified gene samples, the liquid receiving device includes a liquid receiving plate 3101, an installation shaft 3103, a liquid receiving rack 3104, and a rotary driving member for driving the liquid receiving rack 3104 to rotate, a liquid receiving tank 3102 is provided on the liquid receiving plate 3101, the installation shaft 3103 and the translation device I30 are mounted on the same mounting plate C as the clamping jaw a1601, a first end of the liquid receiving rack 3104 is rotatably mounted on the installation shaft 3103, a second end of the liquid receiving rack 3104 is fixedly connected with the liquid receiving plate 3101, and the translation device I30 is used for changing the relative positions of the liquid receiving plate 3101 and the pipetting device B29 in the dripping direction of the pipetting needle.
In this embodiment, when micropipetting, the translation assembly I30 and the rotation driving member are simultaneously activated, the pipetting member B29 moves toward the dripping direction relative to the liquid receiving plate 3101, the rotation driving member drives the liquid receiving frame 3104 to rotate, and drives the liquid receiving plate 3101 to rotate toward the direction away from the pipetting member B29; when receiving liquid, the translation assembly I30 and the rotary driving piece are started simultaneously, the translation assembly I30 moves towards the reverse direction of the dropping direction relative to the liquid receiving plate 3101, the rotary driving piece drives the liquid receiving frame 3104 to rotate and drives the liquid receiving plate 3101 to rotate towards the direction close to the liquid transferring piece B29, and when the liquid receiving frame 3102 of the liquid receiving plate 3101 rotates to the position, the liquid receiving groove 3102 of the liquid receiving plate 3101 is positioned right below the needle head of the liquid transferring piece B29, and the liquid dropped from the liquid transferring piece B29 is collected.
As shown in fig. 14 and 15, the rotary driving member includes a groove 3105, a boss 3106 and a torsion spring 3107, the groove 3105 is provided on the liquid-receiving rack 3104, the boss 3106 is fixedly provided on the liquid-transferring member B29, the boss 3106 is always in pressing contact with the liquid-receiving rack 3104, a first end of the torsion spring 3107 is fixedly connected with a first end of the liquid-receiving rack 3104, a second end of the torsion spring 3107 is fixedly connected with the mounting shaft 3103, and the torsion spring 3107 is always in a stored energy state.
In this embodiment, when micropipetting, the translation assembly I30 is activated, the pipetting piece B29 is moved relative to the liquid receiving plate 3101 in the direction of dripping, at this time, the boss 3106 is moved away from the groove 3105 of the liquid receiving rack 3104, so that the distance between the pipetting piece B29 and the liquid receiving rack 3104 is increased, and since the liquid receiving rack 3104 is rotatably mounted on the mounting shaft 3103, the liquid receiving rack 3104 rotates the liquid receiving plate 3101 in the direction away from the pipetting piece B29, so that the liquid receiving plate 3101 is away from the position under the needle of the pipetting piece B29, and the pipetting piece B29 can perform normal pipetting operation, while the torsion spring 3107 is further deformed to store energy; when receiving liquid, the translation assembly I30 is started, the pipetting piece B29 moves in the opposite direction of the dripping direction relative to the liquid receiving plate 3101, at this time, the boss 3106 moves to be close to the groove 3105 of the liquid receiving rack 3104, the distance between the pipetting piece B29 and the liquid receiving rack 3104 is reduced, the extrusion acting force between the boss 3106 and the liquid receiving rack 3104 is reduced, therefore, the torsion spring 3107 recovers deformation to release energy, the liquid receiving rack 3104 is driven to rotate, the liquid receiving plate 3101 is driven to rotate in the direction close to the pipetting piece B29, when the torsion spring rotates to reach the position, the boss 3106 is positioned in the groove 3105 of the liquid receiving rack 3104, the liquid receiving groove 3102 of the liquid receiving plate 3101 is positioned under the needle head of the pipetting piece B29, the liquid dropped by the pipetting piece B29 is received, the liquid dropped in the pipetting piece B29 is avoided, and the pollution is caused to the sample and gene detection equipment in operation.
In some embodiments, the rotational drive member is a rack and pinion gear fixedly mounted to the first end of the pipetting frame 3104, the rack and pinion gear are in meshing engagement, and the rack and pinion gear are fixedly mounted to the pipetting device B29.
When micro pipetting is carried out, the translation assembly I30 is started, the pipetting piece B29 moves towards the dripping direction relative to the liquid receiving plate 3101, at the moment, the rack moves along with the pipetting piece B29, the rack is meshed with the gear to drive the liquid receiving rack 3104 and the liquid receiving plate 3101 to rotate towards the direction far away from the pipetting piece B29, so that the liquid receiving plate 3101 is far away from the position below the needle head of the pipetting piece B29, and the pipetting piece B29 can carry out normal pipetting operation; when receiving liquid, the translation assembly I30 is started, the liquid transferring piece B29 moves in the opposite direction of the liquid receiving plate 3101 towards the dripping direction, at the moment, the rack moves in the opposite direction along with the liquid transferring piece B29, the rack is meshed with the gear to drive the liquid receiving rack 3104 and the liquid receiving plate 3101 to rotate towards the direction close to the liquid transferring piece B29, when the rack moves to the position, the liquid receiving groove 4 of the liquid receiving plate 3101 is positioned under the needle head of the liquid transferring piece B29, the liquid dropped from the liquid transferring piece B29 is received and collected, the liquid dropping in the liquid transferring piece B29 is avoided, and the pollution to a sample and gene detection equipment in operation is avoided.
As shown in fig. 1, 16 and 17, the amplification and detection module further comprises at least two sets of metal bath modules, at least two sets of optical detectors 1501 and at least two sets of translation assemblies C1504, each metal bath module comprises a hot cover assembly 1503 and a heating base 1502, the hot cover assembly 1503 is installed at the detection port of the optical detector 1501, and the moving action end of the translation assembly C1504 acts on one heating base 1502.
In this embodiment, the transfer assembly 12 transfers the PCR plate on the transfer end of the logistics transportation module C to the heating base 1502, the translation assembly C1504 moves the heating base 1502 to move the PCR plate to a position right below the heat cover assembly 1503, then metal bath is performed to amplify the gene sample, the optical detector 1501 performs optical detection analysis on the amplified gene sample, after amplification detection is completed, the translation assembly C1504 moves the heating base 1502 to move the PCR plate out, and then the transfer assembly 12 and the clamping jaw a separate the PCR plate from the heating base 1502 and perform waste disposal.
As shown in fig. 18, 19, 20 and 21, the logistics transportation module a, the logistics transportation module B and the logistics transportation module C each include a bracket 22 for receiving PCR plates, the logistics transportation module a and the logistics transportation module B further include a translation assembly D21, the translation assembly D21 includes a motor D2101, a sliding rail 2106, a driving wheel D2102, a driven wheel D2103, a driving belt D2104 and a mounting plate D2105, the mounting plate D2105 is fixedly connected with the driving belt D2104, a rotating shaft of the motor D2101 is fixedly connected with a rotating center of the driving wheel D2102, the driving wheel D2102 is driven by the driving wheel D2103 through the driving belt D2104, the mounting plate D2105 is slidably mounted in the sliding rail 2106, the sliding rail 2106 is fixedly mounted in the mounting chamber, wherein the logistics transportation module a and the logistics transportation module C include a lifting assembly B23 for lifting the height of the bracket 22, the lifting assembly B23 is fixedly mounted on the mounting plate D2105, the logistics transportation module B further includes a translation assembly E24, the translation assembly E24 is fixedly arranged on the mounting plate D2105, and two ends of a bracket in the logistics transportation module B are respectively used for receiving a PCR plate containing an amplification reagent and a PCR plate containing a gene sample; during transportation, the PCR plate filled with the amplification reagent is transported to the logistics transportation module B by the logistics transportation module A at the second switch door assembly A, the PCR plate filled with the gene sample is transported to the logistics transportation module C by the logistics transportation module B at the second switch door assembly B, and the lifting action end of the lifting assembly B of the logistics transportation module C is positioned under the first switch door assembly C9.
Clamping blocks are arranged on the brackets of the logistics transportation module A and the logistics transportation module C, grooves are arranged on the PCR plate and the film sealing plate, and the brackets of the logistics transportation module A and the logistics transportation module C can receive the PCR plate through the clamping blocks and the grooves; the transfer process from the logistics transportation module A to the logistics transportation module B is as follows: the first end of the bracket of the logistics transportation module B moves into the second installation chamber A4 through the translation assembly D21 and the translation assembly E24, the bracket of the logistics transportation module A receives the PCR plate, the height of the bracket is raised through the lifting assembly B23 to enable the bracket to be located above the bracket of the logistics transportation module B, then the PCR plate is moved through the translation assembly D21, the bracket in the logistics transportation module B is located right below the bracket of the logistics transportation module A, and the height of the bracket is lowered through the lifting assembly B23 of the logistics transportation module A to enable the PCR plate to be transferred to the logistics transportation module B from the logistics transportation module A;
the transfer flow from the logistics transportation module B to the logistics transportation module C is as follows: the lifting assembly B23 of the logistics transportation module C lowers the height of the bracket to enable the bracket to be positioned below the bracket in the logistics transportation module B, the second end of the bracket of the logistics transportation module C moves into the second installation chamber C6 through the translation assembly D21 and the translation assembly E24, the second end of the bracket in the logistics transportation module B is positioned right above the bracket of the logistics transportation module C, and then the lifting assembly B23 of the logistics transportation module C raises the height of the bracket, so that the PCR plate is transferred to the logistics transportation module C from the logistics transportation module B.
As shown in fig. 22, the airflow control system includes three airflow pieces, the three airflow pieces are respectively used for controlling the internal air pressures of the first installation chamber a1, the first installation chamber B2 and the first installation chamber C3, and each airflow piece includes an induced draft fan B and an induced draft fan C; the air outlet of the induced draft fan B is communicated with the air inlet of the installation chamber, the air inlet of the induced draft fan C is communicated with the air outlet of the installation chamber, and the air inlet of the induced draft fan B and the air inlet of the induced draft fan C are both provided with high-efficiency filters B; the air pressure of the first installation chamber a1, the first installation chamber B2 and the first installation chamber C3 is reduced in sequence, the air pressure of the first installation chamber a1 is greater than the air pressure outside the safety cabinet, and the air pressure of the first installation chamber C3 is less than the air pressure outside the safety cabinet.
In this embodiment, all be provided with high efficiency filter B through draught fan B's air inlet and draught fan C's air inlet in order to prevent air pollution, simultaneously draught fan B and draught fan C all can the speed governing, control first installation room A1, the pressure size of first installation room B2 and first installation room C3, need guarantee from first installation room A1, the pressure differential of first installation room B2 and first installation room C3 subtracts 5-20KPa progressively in proper order, the atmospheric pressure of first installation room A1 is greater than the outer atmospheric pressure of safety cabinet, the atmospheric pressure of first installation room C3 is less than the outer atmospheric pressure of safety cabinet.
In addition, when a scene of high-risk viruses and the like needs to be tested, the air in the first installation chamber B2 in the equipment needs to be prevented from polluting the outside air, the safety of testers is protected, the speed of the induced draft fan B and the induced draft fan C is adjusted to control the air pressure of the first installation chamber B2 to be smaller than the air pressure outside the safety cabinet, when the placing rack C1401, the placing rack D1402, the placing rack E1403 and the placing rack F1404 are pulled, the outside air flows into the equipment from the opening of the first installation chamber B2, but an exhaust fan for exhausting air is arranged at the opening of the first installation chamber B2, and the exhaust fan exhausts the outside air and does not pollute the first installation chamber B2; when the scene tests such as high sensitivity are needed, the first installation chamber B2 in the outside air pollution equipment needs to be avoided, and the air pressure of the first installation chamber B2 controlled by the speed regulation of the induced draft fan B and the induced draft fan C is greater than the air pressure outside the safety cabinet, so that the air inflow is avoided.
As shown in FIG. 1, FIG. 3 and FIG. 10, the gene assaying device further comprises a solid-liquid waste discharging unit 25, the solid-liquid waste discharging unit 25 is installed in the first installation chamber B2, the solid-liquid waste discharging unit 25 comprises a solid waste discharging chamber for discharging solid waste, a liquid waste discharging chamber for discharging liquid waste, a translation unit G2501 and a cover plate 2502, the moving action end of the translation unit G2501 acts on the cover plate 2502, and the cover plate 2502 covers the upper ends of the liquid waste discharging chamber and the solid waste discharging chamber.
In this embodiment, when the replacement lance tip a and the replacement lance tip B are used for discharging waste, the translation assembly G2501 moves the cover plate 2502, so that the solid waste discharge chamber is in an open state, the replacement lance tip a and the replacement lance tip B can be used for discharging waste into the solid waste discharge chamber, and after the waste discharge is completed, the translation assembly G2501 reversely moves the cover plate 2502 to close the solid waste discharge chamber; when liquid waste discharge is performed, the translation assembly G2501 moves the cover plate 2502 so that the liquid waste discharge chamber is in an open state, the liquid-transferring member a1204 discharges the liquid waste into the liquid waste discharge chamber, and after the discharge is completed, the translation assembly G2501 moves the cover plate 2502 in the reverse direction so that the solid waste discharge chamber is closed.
As shown in fig. 1, 10, and 12, the genetic testing apparatus further includes a heat sealing machine 2802 and a translation assembly H2804, the heat sealing machine 2802 and the translation assembly H2804 are installed in the heat sealing machine 28, the heat sealing machine 28 is provided with a third switch assembly 2801, the translation assembly H2804 is used for moving the film sealing plate and the PCR plate to the lower end of the heating plate of the heat sealing machine 2802, a moving action end of the translation assembly H2804 is fixedly provided with a mounting plate F2803, the PCR plate and the film sealing plate are placed on the mounting plate F2803, the mounting plate F2803 is in pressing contact with the third switch assembly 2801, and the rebound direction of the third switch assembly 2801 faces the heat sealing machine 2802.
As shown in FIG. 1, the gene assaying device further comprises a waste chamber installed in the second installation chamber C6, and a first opening and closing door assembly D is provided between the first installation chamber C3 and the waste chamber.
As shown in fig. 1, 2, 9 and 10, each of the first switching door assembly a7, the first switching door assembly B8, the first switching door assembly C9, the first switching door assembly D, the second switching door assembly a10 and the second switching door assembly B11 includes a door panel and an elastic member, two ends of the elastic member are respectively and fixedly connected with the first ends of the installation chamber and the door panel, the elastic members of the first switching door assembly a7, the first switching door assembly B8, the first switching door assembly C9 and the first switching door assembly D are deformed to drive the door panel to rotate upward, and the elastic members of the second switching door assembly a10 and the second switching door assembly B11 are deformed to drive the door panel to rotate toward the second installation chamber B5.
In the present embodiment, the transfer assembly 12 controls the downward movement of the clamping jaw a16, so that the first switching door assembly a7, the first switching door assembly B8, the first switching door assembly C9 and the first switching door assembly D are opened, and the PCR plate is transferred between the upper installation chamber and the lower installation chamber; after the transfer is completed, the transfer assembly 12 controls the clamping jaw A16 to move upwards, under the action of the elastic piece, the first switch door assembly A7, the first switch door assembly B8, the first switch door assembly C9 and the first switch door assembly D can be automatically closed, the second switch door assembly A10 and the second switch door assembly B11 are opened through the logistics transport module B, the PCR is transferred among the three installation chambers at the lower layer, after the transfer is completed, the logistics transport module B moves towards the direction far away from the second switch door assembly A10 or the second switch door assembly B11, and under the action of the elastic piece, the second switch door assembly A10 and the second switch door assembly B11 can be automatically closed.
The translation assembly A1703, the translation assembly B2005, the translation assembly C1504, the translation assembly E24, the translation assembly F, the translation assembly G2501, the translation assembly H2804 and the translation assembly I30 are all any one of an air cylinder, an oil cylinder, a linear motor, a screw-nut mechanism and a gear rack.
The utility model discloses gene detection equipment's work flow as follows:
inserting a placing rack A1301 and a placing rack B1302 into the first installation chamber A1, enabling the sample adding module to change the position of a liquid transferring piece A1204 in three directions of XYZ through a transferring assembly 12, so as to achieve the purpose of transferring amplification reagents into micropores of a PCR plate, then controlling a clamping jaw control piece A16 to grab the PCR plate filled with the amplification reagents, moving the PCR plate to be positioned right above a first switch door assembly A7 through the transferring assembly 12, then controlling a clamping jaw A16 to move downwards through the transferring assembly 12, enabling the first switch door assembly A7 to be opened, placing the PCR plate on a bracket 22 of a logistics transportation module A, then transferring the PCR plate to a logistics transportation module B through the logistics transportation module A, and enabling the logistics transportation module B to transfer the PCR plate to be right below a first switch door assembly B8;
in the first installation chamber B2, the gripper jaw a16 is moved by the transfer assembly 12 to be located right above the first opening and closing door assembly B8, then the gripper jaw a16 is controlled by the transfer assembly 12 to move downward, so that the first opening and closing door assembly B8 is opened and picks up the PCR plate into the first installation chamber B2, the rack C1401, the rack D1402, the rack E1403 and the rack F1404 are inserted, the transfer assembly 12 moves the gripper jaw C1205 to pick up the eight tubes and place the eight tubes in the middle index 1411, the transfer assembly 12 moves the pipette a1204 for transferring the purification reagents, the corresponding purification reagents are transferred into the eight tubes by the pipette a1204, then the transfer assembly 12 moves the gripper jaw C1205 to pick up the sample tubes to the opening and closing assembly 20 for opening the lid, then the gene samples in the sample tubes are transferred into the eight tubes by the pipette a for transferring the gene samples before purification 1204, and then the eight tubes are transferred to the purification module 17 for purification process, after the purification treatment is completed, transferring the purified gene sample into a PCR plate by a transfer element B29, transferring the PCR plate and a film sealing plate onto a mounting plate F2803 by a clamping jaw A, moving the film sealing plate and the PCR plate to the lower end of a heating plate of a heat sealing machine 2802 by a translation assembly H2804, heat sealing the film sealing plate and the PCR plate by the heat sealing machine 2802, transferring the heat sealed PCR by the clamping jaw A to be positioned right above a first switch door assembly B8, controlling the clamping jaw A16 to move downwards by a transfer assembly 12, so that the first switch door assembly B8 is opened, placing the PCR plate at the second end of a bracket 22 in a logistics transportation module B, transferring the PCR plate to a logistics transportation module C by the logistics transportation module B, wherein the transfer action end of the logistics transportation module C is positioned right below the first switch door assembly C9;
in the first installation chamber C3, the transfer assembly 12 moves the clamping jaw a16 to be located right above the first opening and closing door assembly C9, then the transfer assembly 12 controls the clamping jaw a16 to move downwards, so that the first opening and closing door assembly C9 is opened, a PCR plate is grabbed into the first installation chamber C3, the heat-sealed PCR plate is transferred onto the heating base 1502, the translation assembly C1504 moves the heating base 1502, so that the PCR plate moves right below the heat cover assembly 1503, then a metal bath is performed to amplify the gene sample, meanwhile, the optical detector 1501 performs optical detection analysis on the amplified gene sample, after the amplification detection is completed, the translation assembly C1504 moves the heating base 1502, so that the PCR plate is removed, and then the PCR plate is separated from the heating base 1502 through the transfer assembly 12 and the clamping jaw a, and waste discharge treatment is performed.
The technical scheme of the utility model is not limited to the restriction of above-mentioned specific embodiment, all according to the utility model discloses a technical scheme makes technical deformation, all falls into within the protection scope of the utility model.

Claims (21)

1. A gene assaying device, comprising:
a safety cabinet; the safety cabinet is divided into an upper layer and a lower layer which are relatively isolated, each layer of the safety cabinet is divided into three relatively isolated installation chambers, the installation chambers on the upper layer are respectively a first installation chamber A (1), a first installation chamber B (2) and a first installation chamber C (3), and the installation chambers on the lower layer are respectively a second installation chamber A (4), a second installation chamber B (5) and a second installation chamber C (6); a first switching door assembly A (7) is arranged between the first installation chamber A (1) and the second installation chamber A (4), a first switching door assembly B (8) is arranged between the first installation chamber B (2) and the second installation chamber B (5), a first switching door assembly C (9) is arranged between the first installation chamber C (3) and the second installation chamber C (6), a second switching door assembly A (10) is arranged between the second installation chamber A (4) and the second installation chamber B (5), and a second switching door assembly B (11) is arranged between the second installation chamber B (5) and the second installation chamber C (6);
an airflow control system; the air flow control system is used for controlling the air pressure conditions of the first installation chamber A (1), the first installation chamber B (2) and the first installation chamber C (3);
a first processing module (13) for processing amplification reagents and PCR plates;
a second processing module (14) for processing the reagent, the tip and the gene sample;
the sample adding module is used for adding an amplification reagent into the micropores of the PCR plate;
the purification processing module is used for processing the gene sample;
an amplification detection module (15) for gene amplification and detection;
a heat sealer (2802) for sealing the PCR plate;
a logistics transportation module A;
a logistics transportation module B;
a logistics transportation module C;
the sample adding module and the first processing module (13) are installed in a first installation chamber A (1), the purifying processing module, the second processing module (14) and the heat sealing machine are installed in a first installation chamber B (2), the amplification detection module (15) is installed in a first installation chamber C (3), and the logistics transportation module A, the logistics transportation module B and the logistics transportation module C are installed in a second installation chamber A (4), a second installation chamber B (5) and a second installation chamber C (6) respectively; the logistics transportation module A, the logistics transportation module B and the logistics transportation module C are respectively positioned below the sample adding module, the purification processing module and the amplification detection module (15); during transportation, the PCR plate with the amplification reagent passes through the first switch door component A (7) and is arranged at the transfer action end of the logistics transportation module A through the sample adding module, when the logistics transportation module A transfers the PCR plate to the second switch door component A (10), the PCR plate is arranged at the transfer action end of the logistics transportation module B, when the logistics transportation module B transfers the PCR plate to the first switch door component B (8), the PCR plate enters the first installation chamber B (2) through the purification treatment module, the purified gene sample is added into the PCR plate, after the heat sealing machine (2802) is subjected to heat sealing, the PCR plate with the gene sample is arranged at the transfer action end of the logistics transportation module B through the first switch door component B (8) of the purification treatment module, when the logistics transportation module B transfers the PCR plate to the second switch door component B (11), the PCR plate is arranged at the transfer action end of the logistics transportation module C, when the logistics transportation module C transfers the PCR plate to the first switch door component C (9), the gene sample in the PCR plate is subjected to gene detection by the amplification detection module (15) after entering the first installation chamber C (3) through the purification processing module.
2. The gene assaying device according to claim 1, wherein the loading module, the purification processing module and the amplification detecting module each comprise a transfer assembly (12), a jaw a (16) for transferring the PCR plate, and a jaw control a (1601) for controlling the jaw a (16) to open and close, wherein the loading module and the purification processing module further comprise a pipette a (1204) for transferring the liquid, and each transfer assembly (12) comprises:
an X-direction moving assembly (1201) for X-direction movement; the X-direction moving assembly comprises a motor A, a driving wheel A, a driven wheel A, a transmission belt A and a mounting plate A, wherein the mounting plate A is fixedly connected with the transmission belt A, a rotating shaft of the motor A is fixedly connected with a rotating center of the driving wheel A, and the driving wheel A and the driven wheel A are transmitted through the transmission belt A;
a Y-direction moving component (1202) for Y-direction movement; the Y-direction moving assembly comprises a motor B, a driving wheel B, a driven wheel B, a transmission belt B and a mounting plate B, the mounting plate B is fixedly connected with the transmission belt B, a rotating shaft of the motor B is fixedly connected with a rotating center of the driving wheel B, and the driving wheel B and the driven wheel B are transmitted through the transmission belt B;
a Z-direction moving component (1203) for Z-direction movement; the Z-direction moving assembly comprises a motor C, a lead screw nut mechanism A and a mounting plate C, a rotating shaft of the motor C is fixedly connected with a lead screw A of the lead screw nut mechanism A, the mounting plate C is fixedly connected with a nut A of the lever nut mechanism A, and a clamping jaw control piece A (1601) and a liquid transferring piece A (1204) are fixedly mounted on the mounting plate C;
the X-direction moving assembly (1201) of the sample adding module and the amplification detection module is arranged on the mounting plate B, and the Z-direction moving assembly (1203) is fixedly arranged on the mounting plate A; and a Y-direction moving assembly (1202) of the purification processing module is arranged on the mounting plate A, and a Z-direction moving assembly (1203) is fixedly arranged on the mounting plate B.
3. The gene testing apparatus according to claim 2, wherein the first processing module (13) comprises at least two sets of a rack A (1301), a rack B (1302) and a cooling module A (1303) for cooling amplification reagents, the test tubes containing the amplification reagents are placed on the rack A (1301), the number of the amplification reagents on one set of the rack A (1301) is not less than that required by a gene sample experiment, the PCR plate is placed on the rack B (1302), an opening is formed in a side surface of the first installation chamber A (1), both the rack A (1301) and the rack B (1302) can be placed in the first installation chamber A (1), and the cooling module A (1303) is installed right below the rack A (1301).
4. The gene detection device according to claim 3, wherein the second processing module (14) comprises at least two sets of placing racks C (1401), at least two sets of placing racks D (1402), placing racks E (1403), at least two sets of temporary storage racks (1405), placing racks F (1404), a vibrating piece A (1412) for vibrating a magnetic bead solution, a heating module (1406) for heating a lysate and a cooling module B for cooling a purified gene sample, wherein the purifying reagent, the eight connecting pipes for purification reaction and the replacement gun heads A are all placed on the placing racks C (1401), the number of the purifying reagent, the number of the eight connecting pipes and the number of the replacement gun heads A on one set of placing racks C (1401) are respectively not less than the number of the purifying reagent, the number of the eight connecting pipes and the number of the replacement gun heads A required by one group of gene sample experiments, the sample pipes containing the gene sample are placed on the placing racks D (1402), the number of gene samples on one set of rack D (1402) is not less than the number of gene samples required by a group of gene sample experiments, a cleaning solution for eluting the gene samples is placed in the rack E (1403), a replacement gun head B and a film sealing plate are both placed in the rack F (1404), a PCR plate not filled with purified gene samples is placed on a temporary storage rack (1405), a cooling module B is installed under the temporary storage rack (1405), an opening is formed in the side surface of a first installation chamber B (2), the rack C (1402), the rack D (1402), the rack E (1403) and the rack F (1404) can be placed in the first installation chamber B (2) in a drawer type mode.
5. The gene detection device according to claim 4, wherein the purification processing module further comprises at least one purification module (17), a high-efficiency filter A (18) and an induced draft fan A (19), each purification module (17) comprises a heating oscillation chamber (1701), a magnetic attraction member (1702), a vibration member B (1705), a translation assembly A (1703) and a heating member, the heating oscillation chamber (1701) is provided with a reaction mounting frame (1704), eight tubes for purification reaction are placed on the reaction mounting frame (1704), the heating action end of the heating member acts on the eight tubes, the heating member is mounted at the vibration action end of the vibration member B (1705), the translation assembly A (1703) is used for moving the magnetic attraction member (1702) to change the attraction force of the magnetic attraction member (1702) on the magnetic beads in the eight tubes, the side edge of the heating oscillation chamber (1701) is provided with an air outlet, one of the air inlets of the high-efficiency filter A (18) is communicated with an air outlet of the heating oscillation chamber (1701), the air inlet of the induced draft fan A (19) is communicated with the air outlet of the high-efficiency filter A (18).
6. The gene detecting apparatus according to claim 4, wherein the purification processing module further comprises an opening and closing assembly (20) for opening and closing the cap in the sample tube, the opening and closing assembly comprises a lifting assembly A (2001), a clamping jaw B (2002), a clamping jaw control member B for controlling the clamping jaw B (2002) to open and close, and a tube fixing member for fixing the tube in the sample tube, the lifting action end of the lifting assembly A (2001) acts on the clamping jaw control member B, and the tube fixing member is disposed below the clamping jaw B (2002).
7. The gene detection device according to claim 6, wherein the tube fixing member comprises a fixing block A (2003), a fixing block B (2004) and a translation assembly B (2005) for controlling the distance between the fixing block A (2003) and the fixing block B (2004), the fixing block B (2004) is installed right below the clamping jaw B (2002), the moving action end of the translation assembly B (2005) acts on the fixing block A (2003), the side faces of the fixing block A (2003) opposite to the fixing block B (2004) are both arc faces, and a clamping ring (2006) for clamping and hanging the sample tube is arranged at the upper end of the arc face of the fixing block A (2003).
8. The gene testing apparatus according to claim 2, wherein the amplification test module further comprises at least two sets of metal bath modules, at least two sets of optical testing instruments (1501) and at least two sets of translation modules C (1504), each metal bath module comprises a heat cover assembly (1503) and a heating base (1502), one set of heat cover assembly (1503) is installed at the testing port of one set of optical testing instruments (1501), and the moving action end of one set of translation modules C (1504) acts on one heating base (1502).
9. The gene detection device of claim 1, wherein each of the logistics transportation module A, the logistics transportation module B and the logistics transportation module C comprises a bracket (22) for receiving a PCR plate, each of the logistics transportation module A and the logistics transportation module B further comprises a translation assembly D (21), each translation assembly D (21) comprises a motor D (2101), a sliding rail (2106), a driving wheel D (2102), a driven wheel D (2103), a driving belt D (2104) and a mounting plate D (2105), each mounting plate D (2105) is fixedly connected with each driving belt D (2104), a rotating shaft of each motor D (2101) is fixedly connected with a rotating center of each driving wheel D (2102), each driving wheel D (2102) is in transmission with each driven wheel D (2103) through each driving belt D (2104), each mounting plate D (2105) is slidably mounted in each sliding rail (2106), each sliding rail (2106) is fixedly mounted in each mounting chamber, each logistics transportation module A and each logistics transportation module C comprises a lifting assembly for lifting and lowering the height of the brackets (22) B (23), the lifting component B (23) is fixedly arranged on the mounting plate D (2105), the logistics transportation module B further comprises a translation component E (24) used for moving the bracket (22), the translation component E (24) is fixedly arranged on the mounting plate D (2105), and two ends of the bracket in the logistics transportation module B are respectively used for receiving a PCR plate containing an amplification reagent and a PCR plate containing a gene sample; during transportation, the PCR plate filled with the amplification reagent is transferred to the logistics transportation module B by the logistics transportation module A at the second switch door assembly A, the PCR plate filled with the gene sample is transferred to the logistics transportation module C by the logistics transportation module B at the second switch door assembly B, and the lifting action end of the lifting assembly B of the logistics transportation module C is positioned under the first switch door assembly C (9).
10. The gene assaying device according to claim 1, wherein the airflow control system comprises three airflow members for controlling the internal air pressures of the first installation chamber a (1), the first installation chamber B (2), and the first installation chamber C (3), respectively, each airflow member comprising an induced draft fan B and an induced draft fan C; the air outlet of the induced draft fan B is communicated with the air inlet of the installation chamber, the air inlet of the induced draft fan C is communicated with the air outlet of the installation chamber, and the air inlet of the induced draft fan B and the air inlet of the induced draft fan C are both provided with high-efficiency filters B; the air pressure of the first installation chamber A (1), the air pressure of the first installation chamber B (2) and the air pressure of the first installation chamber C (3) are sequentially reduced, the air pressure of the first installation chamber A (1) is greater than the air pressure outside the safety cabinet, and the air pressure of the first installation chamber C (3) is smaller than the air pressure outside the safety cabinet.
11. The genetic testing device of claim 4, wherein the second processing module further comprises at least two sets of translation assemblies F and at least two sets of air inducing assemblies (1408) for absorbing the aerosol generated by the genetic sample, wherein one set of translation assemblies F is used for changing the distance between the air inlet of the air inducing assemblies (1408) and the temporary storage rack (1405), and the air inlet of one set of air inducing assemblies (1408) is positioned right above one set of temporary storage rack (1405) during air induction.
12. The genetic testing device according to claim 1, wherein the genetic testing device further comprises a solid-liquid waste discharge member (25), the solid-liquid waste discharge member (25) is installed in the first installation chamber B (2), the solid-liquid waste discharge member (25) comprises a solid waste discharge chamber for discharging solid waste, a liquid waste discharge chamber for discharging liquid waste, a translation member G (2501) and a cover plate (2502), the movement action end of the translation member G (2501) acts on the cover plate (2502), and the cover plate (2502) covers the upper ends of the liquid waste discharge chamber and the solid waste discharge chamber.
13. The gene assaying device according to claim 4, wherein the rack D (1402) is provided with a plurality of placing grooves (1410) for placing the sample tubes, one side of the placing grooves (1410) is provided with a spring piece (1409), the direction in which the spring piece (1409) recovers deformation is directed toward the central axis of the placing grooves (1410), and when the sample tubes are placed in the placing grooves (1410), the spring piece (1409) is in pressing contact with the side walls of the sample tubes.
14. The gene assaying device according to claim 2, wherein the purification treatment module further comprises two sets of transfer units (12), a jaw C (1205) for transferring the sample tube and the eight-linked tube, and a jaw control C for controlling the jaw C (1205) to open and close, and two sets of pipettes A (1204) for transferring the liquid, three pipettes A (1204) of the purification treatment module being used for transferring the purification reagent, the washing liquid, and the gene sample, respectively, a pipetting member A (1204) for transferring the cleaning liquid and a jaw control member A (1601) are mounted on a mounting plate C of the same transfer assembly (12), a pipetting member A (1204) for transferring a gene sample and a jaw control member C are mounted on a mounting plate C of the same transfer unit (12), a pipetting member A (1204) for transferring the purifying agent is mounted on a mounting plate C of another transfer unit (12).
15. The gene assaying device according to claim 1, further comprising a heat sealing cassette (28) and a translation assembly H (2804), the heat sealing machine (2802) and the translation assembly H (2804) being installed in the heat sealing cassette (28), the heat sealing cassette (28) being provided with a third switch assembly (2801), the translation assembly H (2804) being used to move the sealing plate and the PCR plate to a lower end of a heating plate of the heat sealing machine (2802), a moving action end of the translation assembly H (2804) being fixedly provided with a mounting plate F (2803), the PCR plate and the sealing plate being placed on the mounting plate F (2803), the mounting plate F (2803) being in pressing contact with the third switch assembly (2801), a spring back direction of the third switch assembly (2801) facing the heat sealing machine (2802).
16. The gene assaying device according to claim 14, wherein the purification processing module further comprises a pipetting member B (29) for micropipetting, a translation assembly I (30), and a liquid receiving assembly (31) for receiving liquid from the pipetting member B, the liquid receiving assembly comprising a liquid receiving plate (3101), the liquid transferring device comprises an installation shaft (3103), a liquid receiving frame (3104) and a rotary driving piece for driving the liquid receiving frame (3104) to rotate, wherein a liquid receiving groove (3102) is arranged on a liquid receiving plate (3101), the installation shaft (3103) and a translation assembly I (30) are arranged on the same installation plate C together with a clamping jaw control piece A (1601), the first end of the liquid receiving frame (3104) is rotatably arranged on the installation shaft (3103), the second end of the liquid receiving frame (3104) is fixedly connected with the liquid receiving plate (3101), and the translation assembly I (30) is used for changing the relative position of the liquid receiving plate (3101) and a liquid transferring piece B (29) in the liquid transferring needle liquid dropping direction.
17. The gene assaying device according to claim 16, wherein the rotary driving member comprises a groove (3105), a boss (3106) and a torsion spring (3107), the groove (3105) is disposed on the liquid-receiving rack (3104), the boss (3106) is fixedly disposed on the liquid-transferring member B (29), the boss (3106) is always in pressing contact with the liquid-receiving rack (3104), a first end of the torsion spring (3107) is fixedly connected with a first end of the liquid-receiving rack (3104), a second end of the torsion spring (3107) is fixedly connected with the mounting shaft (3103), and the torsion spring (3107) is always in a stored energy state.
18. The gene assaying device according to claim 4, further comprising a plurality of sets of fixing means for fixing the rack A (1301), the rack B (1302), the rack C (1401), the rack D (1402) and the rack E (1403), respectively, wherein each fixing means comprises an in-place sensor and an electromagnet, and when the in-place sensor detects that the corresponding rack is inserted in place, the electromagnet adsorbs and fixes the corresponding rack.
19. The gene assaying device according to claim 7, wherein a high friction cushion (2007) is fixedly provided on the arc surface of each of the fixed block A (2003) and the fixed block B (2004).
20. The gene assaying device according to claim 1, further comprising a waste chamber installed in the second installation chamber C (6), and a first opening/closing door assembly D is provided between the first installation chamber C (3) and the waste chamber.
21. The gene detecting equipment of claim 20, wherein each of the first switching door assembly a (7), the first switching door assembly B (8), the first switching door assembly C (9), the first switching door assembly D, the second switching door assembly a (10) and the second switching door assembly B (11) comprises a door plate and an elastic member, two ends of the elastic member are respectively and fixedly connected with the installation chamber and the first end of the door plate, the elastic members of the first switching door assembly a (7), the first switching door assembly B (8), the first switching door assembly C (9) and the first switching door assembly D drive the door plate to rotate towards the upper side when being restored to be deformed, and the elastic members of the second switching door assembly a (10) and the second switching door assembly B (11) drive the door plate to rotate towards the second installation chamber B (5) when being restored to be deformed.
CN202022322757.6U 2020-10-19 2020-10-19 Gene detecting apparatus Active CN212504894U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113375995A (en) * 2021-06-29 2021-09-10 上海汇像信息技术有限公司 Full-automatic filtration system
CN114350496A (en) * 2020-10-19 2022-04-15 成都瀚辰光翼生物工程有限公司 Gene detecting apparatus
CN114369524A (en) * 2022-03-18 2022-04-19 安永医疗科技常州有限公司 Nucleic acid detection system

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114350496A (en) * 2020-10-19 2022-04-15 成都瀚辰光翼生物工程有限公司 Gene detecting apparatus
WO2022083528A1 (en) * 2020-10-19 2022-04-28 成都瀚辰光翼生物工程有限公司 Genetic testing device
CN114350496B (en) * 2020-10-19 2023-12-08 成都瀚辰光翼生物工程有限公司 Gene detection apparatus
CN113375995A (en) * 2021-06-29 2021-09-10 上海汇像信息技术有限公司 Full-automatic filtration system
CN114369524A (en) * 2022-03-18 2022-04-19 安永医疗科技常州有限公司 Nucleic acid detection system

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