CN219059002U - Multi-station nucleic acid extractor - Google Patents

Multi-station nucleic acid extractor Download PDF

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Publication number
CN219059002U
CN219059002U CN202223142071.4U CN202223142071U CN219059002U CN 219059002 U CN219059002 U CN 219059002U CN 202223142071 U CN202223142071 U CN 202223142071U CN 219059002 U CN219059002 U CN 219059002U
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China
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plate
magnetic rod
silica gel
module
nucleic acid
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CN202223142071.4U
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Chinese (zh)
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蒋如飞
杨小岚
宋建军
潘超
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Wuhan Naci Biotechnology Co ltd
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Wuhan Naci Biotechnology Co ltd
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Abstract

The utility model discloses a multi-station nucleic acid extractor which comprises a workbench, a magnetic rod sleeve module, a magnetic rod module and a heating module, wherein the magnetic rod sleeve module, the magnetic rod module and the heating module are arranged on the workbench, the heating module comprises a fixed plate, a hole groove plate, a heating block, an upper silica gel plate, a lower silica gel plate, a heat insulation plate and a temperature measuring circuit, the heat insulation plate is made of heat insulation materials, the upper silica gel plate and the lower silica gel plate are respectively made of heat conducting silica gel and heat insulation silica gel, the hole groove plate is provided with a plurality of holes, the fixed plate is used for receiving the lower silica gel plate, the lower silica gel plate is used for receiving the heating block, the heating block is used for receiving the upper silica gel plate, the temperature measuring circuit is contacted with the bottom surface of the hole groove plate and is used for sending the temperature of the hole groove plate to a controller, and therefore the heating value of the heating block is adjusted through the controller. The silica gel plate can uniformly transfer heat to the well plate, and prevent the well plate from being locally overheated or nonuniform in temperature distribution, so that the nucleic acid extraction reagent in the deep well plate can be uniformly heated, and the quality of extracted nucleic acid is improved.

Description

Multi-station nucleic acid extractor
Technical Field
The utility model belongs to the field of nucleic acid extractors, and in particular relates to a multi-station nucleic acid extractor.
Background
The nucleic acid extraction principle of some nucleic acid extractors on the market at present is based on a mature magnetic bead method, and mainly depends on electrostatic action, hydrophobic action and hydrogen bonding action. The cells or tissues are subjected to lysis and the DNA/RNA therein is released. The superparamagnetism silica nano magnetic beads subjected to surface modification are subjected to specific binding with nucleic acid to form a nucleic acid-magnetic bead complex. Then under the action of the external magnetic field, the compound is separated. Then washing off impurities which are not specifically adsorbed by the eluent, desalting and purifying to obtain the nucleic acid substance to be extracted.
In the process of extracting nucleic acid, it is necessary to heat the deep-well plate so that the solution in the deep-well plate is heated to a constant temperature and the extraction of nucleic acid is performed at the constant temperature. The conventional heating device of the current nucleic acid extractor basically uses a heating plate to heat a region at the bottom of a heated plate, and the heating plate is heated unevenly or has large temperature change, so that the heated plate is heated unevenly or has large temperature change, and the nucleic acid extracting solution in a deep pore plate arranged on the heated plate is heated unevenly and has temperature difference, so that the accuracy of an extraction result is directly influenced. In addition, when the heating plate of the existing nucleic acid extractor is heated, the heat loss is fast, so that the heating efficiency is low, and the comparison cost is caused.
Disclosure of Invention
Aiming at the defects or improvement demands of the prior art, the utility model provides a multi-station nucleic acid extractor which is compact in structure, and a heating module can uniformly heat a hole groove plate and has a good heating effect on a deep hole plate placed on the hole groove plate.
In order to achieve the above object, according to one aspect of the present utility model, there is provided a multi-station nucleic acid extraction apparatus, comprising a workbench, a magnetic rod sleeve module, a magnetic rod module and a heating module, wherein the magnetic rod sleeve module, the magnetic rod module and the heating module are arranged on the workbench, a plurality of station slots are arranged on the workbench, and the heating module is arranged in at least one station slot, and the multi-station nucleic acid extraction apparatus is characterized in that:
the heating module comprises a fixing plate, a hole groove plate, a heating block, an upper silica gel plate, a lower silica gel plate, a heat insulation plate and a temperature measuring circuit, wherein the heat insulation plate is made of heat insulation materials, the upper silica gel plate is made of heat conduction silica gel, the lower silica gel plate is made of heat insulation silica gel, the hole groove plate is provided with a plurality of deep hole pipes for accommodating deep hole plates, groove walls are in contact with the deep hole pipes, the fixing plate is installed on the workbench, the fixing plate is connected with the lower silica gel plate, the lower silica gel plate is connected with the heating block, the heating block is connected with the upper silica gel plate in an electric heating mode, the upper silica gel plate is connected with the hole groove plate in a surface contact mode, the heat insulation plate is fixedly sleeved on the hole groove plate and fixedly installed on the workbench, and the hole groove plate, the heat insulation plate and the fixing plate are respectively in sealing fit with the groove walls of the station groove, and the temperature measuring circuit is connected with the bottom surface of the hole groove plate in a sealing mode for detecting the temperature of the hole groove plate.
Preferably, the upper silica gel plate has two pieces and they are separately arranged for heating different areas of the cell plate;
the lower silica gel plate has two pieces and they are also arranged separately.
Preferably, the station slots are provided with four.
Preferably, the fixing plate and the perforated slot plate are both made of an aluminum alloy.
Preferably, the air self-cleaning system comprises a protective cover shell and an air self-cleaning system, wherein the protective cover shell is installed on the workbench, the air self-cleaning system comprises an ultraviolet sterilizing lamp, an exhaust fan and a filter element, the ultraviolet sterilizing lamp, the exhaust fan and the filter element are installed on the protective cover shell, a fresh air inlet hole is further formed in the workbench, and the filter element is installed at an air outlet of the protective cover shell so as to filter air exhausted by the exhaust fan.
Preferably, the magnetic rod sleeve module comprises a magnetic rod sleeve and a magnetic rod sleeve driving mechanism arranged on the workbench, wherein the magnetic rod sleeve driving mechanism comprises a magnetic rod sleeve driving motor and a belt transmission mechanism, and the magnetic rod sleeve driving motor is connected with the magnetic rod sleeve through the belt transmission mechanism so as to be used for driving the magnetic rod sleeve to move up and down.
Preferably, the magnetic rod module comprises a magnetic rod and a magnetic rod driving mechanism arranged on the workbench, wherein the magnetic rod driving mechanism comprises a magnetic rod driving motor and a screw rod mechanism, and the magnetic rod driving motor is connected with the magnetic rod through the screw rod mechanism so as to be used for driving the magnetic rod to move up and down.
Preferably, the magnetic rod device further comprises a support and a support driving mechanism, wherein the support driving mechanism comprises a support driving motor and a belt transmission mechanism, the support driving motor is connected with the support through the belt transmission mechanism and used for driving the support to horizontally move, and the magnetic rod sleeve module and the magnetic rod module are installed on the support.
Preferably, a plurality of limit sensors are arranged on the bracket and used for limiting the moving stroke of the bracket, the magnetic rod of the magnetic rod module and the magnetic rod sleeve of the magnetic rod sleeve module.
In general, the above technical solutions conceived by the present utility model, compared with the prior art, enable the following beneficial effects to be obtained:
1) The upper silica gel plate of the heating module of the nucleic acid extraction instrument is made of heat-conducting silica gel, and the upper silica gel plate can uniformly transfer heat to the hole groove plate, so that local overheating or uneven temperature distribution of the hole groove plate is prevented, and therefore nucleic acid extraction reagents placed in a deep pore plate of the hole groove plate can be uniformly heated, and the quality of extracted nucleic acid is improved.
2) The magnetic rod sleeve module and the magnetic rod module of the nucleic acid extraction instrument are integrated on the support to form a whole, and are driven by the support driving mechanism to horizontally move, so that different stations can be operated to realize different operations, extraction of nucleic acid is convenient to conduct, the space layout is reasonable, the stations can be reduced, the instrument is more compact, and the requirements of the market on different operation spaces of the instrument are met.
3) The nucleic acid extractor has ultraviolet sterilizing and air self-cleaning system inside, and can run safely and reliably.
4) The nucleic acid extractor is provided with a plurality of safety limit sensors, so that the magnetic rod sleeve module, the magnetic rod module and the bracket move in a safety range during movement.
Drawings
FIG. 1 is a schematic illustration of the present utility model with the protective casing removed;
FIG. 2 is a schematic diagram of a heating module according to the present utility model;
FIG. 3 is a schematic illustration of the air self-cleaning system of the present utility model mounted on a protective enclosure.
Detailed Description
The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model. In addition, the technical features of the embodiments of the present utility model described below may be combined with each other as long as they do not collide with each other.
Referring to fig. 1 to 3, a multi-station nucleic acid extractor comprises a workbench 1, and a magnetic rod sleeve module, a magnetic rod module and a heating module 2 which are arranged on the workbench 1, wherein a plurality of station grooves are formed in the workbench 1, at least one station groove is internally provided with the heating module 2, the magnetic rod sleeve module and the magnetic rod module are all of the existing structure, the magnetic rod sleeve module comprises a magnetic rod sleeve and a magnetic rod sleeve driving mechanism arranged on the workbench 1, the magnetic rod sleeve driving mechanism comprises a magnetic rod sleeve driving motor 46 and a belt transmission mechanism 55, and the magnetic rod sleeve driving motor 46 is connected with the magnetic rod sleeve through the belt transmission mechanism 55 so as to be used for driving the magnetic rod sleeve to move up and down. The magnetic rod module comprises a magnetic rod and a magnetic rod driving mechanism arranged on the workbench 1, wherein the magnetic rod driving mechanism comprises a magnetic rod driving motor 46 and a screw rod mechanism, and the magnetic rod driving motor 46 is connected with the magnetic rod through the screw rod mechanism so as to be used for driving the magnetic rod to move up and down. The magnetic rod sleeve comprises a first base plate 31 and sleeves 34 which are integrally formed, and 96 sleeves 34 are preferable. The magnetic rod comprises a second substrate 20 and rod bodies, the quantity of the rod bodies 25 is consistent with that of the sleeves 34, the rod bodies 25 are provided with medical grade stainless steel capillaries, the materials are stable and corrosion-resistant, the purity of the reagent can be ensured, and the magnetic rod sleeve is a plastic consumable. The magnetic rod sleeve is matched with the magnetic rod for use, and the magnetic rod sleeve is positioned right below the magnetic rod. The transmission mechanism is reasonably and spatially distributed by the two belt transmission mechanisms 55 and the one screw mechanism, the stations can be retracted to four stations, the instrument is more compact, and the requirements of the market on different operation spaces of the instrument are met.
The heating module 2 comprises a fixing plate 105, a slot plate 101, a heating block 103, an upper silica gel plate 104, a lower silica gel plate 107, a heat insulation plate 106 and a temperature measuring circuit 102, wherein the heat insulation plate 106 is made of a heat insulation material, and the heat insulation material can be a traditional heat insulation material such as glass fiber, asbestos, rock wool, silicate and the like, or a novel heat insulation material such as aerogel felt, a vacuum plate and the like. The upper silica gel plate 104 is made of heat-conducting silica gel, the lower silica gel plate 107 is made of heat-insulating silica gel, the hole groove plate 101 is provided with a plurality of deep holes for accommodating deep holes, the groove walls of the holes are in contact with the deep holes, the quantity and arrangement modes of the holes in the hole groove plate 101 are consistent with those of the deep holes, the fixing plate 105 is installed on the workbench 1, the fixing plate 105 is connected with the lower silica gel plate 107, the lower silica gel plate 107 is connected with the heating block 103, the heating block 103 heats in an electric heating mode, the heating block 103 is connected with the upper silica gel plate 104, the upper silica gel plate 104 is connected with the hole groove plate 101, the upper silica gel plate 104 is in surface contact with the hole groove plate 101, so that heat emitted by the heating block 103 is uniformly transmitted to the hole groove plate 101, the heat insulating plate 106 is fixedly sleeved on the hole groove plate 101 and fixedly installed on the workbench 1, heat insulating plate 101 is conducted between the hole groove plate 101 and the workbench 1, the heat insulating block 103 is heated in an electric heating mode, the heat insulating block 103 is connected with the upper silica gel plate 104 in an electric heating mode, and the heat insulating plate 103 can be sealed by the heat insulating plate 101 and the heat insulating plate 104, and the heat insulating plate 103 can be sealed by the heat insulating plate 101, and the heat insulating plate is sealed by the heat insulating plate 101. The upper silica gel plate 104, the heating block 103 and the lower silica gel plate 107 are clamped by the hole groove plate 101 and the fixing plate 105, and the structure is similar to a sandwich structure, so that a good effect of preventing heat loss can be achieved, the upper silica gel plate 104 is made of heat-conducting silica gel, the heat insulation performance is good, the temperature of the hole groove plate 101 can be kept within a set threshold range, and the temperature of the hole groove plate 101 is prevented from being influenced by temperature change of the heating block 103. The temperature measuring circuit 102 is in contact with the bottom surface of the perforated slot plate 101 for detecting the temperature of the perforated slot plate and transmitting the temperature of the perforated slot plate 101 to the controller 202, so that the heat generation amount of the heat block 103 is adjusted by the controller 202. The temperature measurement circuit 102 can transmit the temperature of the heating position to the controller 202, and can realize quantitative display and control of the temperature. The main control board and the driving board of the controller 202 are separately arranged, the main control board and the driving board are connected in a modularized mode through harness terminals which are convenient to separate, the main control board and the driving board are not mutually interfered, when one of the main control board and the driving board has a problem, the main control board and the driving board can be quickly replaced, and the time occupied by fault elimination is reduced. The fixing plate 105 and the well plate 101 are both made of aluminum alloy, so that the well plate 101 can conveniently heat the nucleic acid extraction reagent in the deep well plate. The utility model focuses on improving the heating module 2 of the nucleic acid extractor, so that the heating efficiency is high and the heat preservation effect is good.
The workbench 1 is a workbench for extracting instrument nucleic acid, is made of high-quality aluminum alloy, and is preferably provided with four station grooves, but is not limited to four station grooves. The heating module 2 is arranged in the station groove, the hole groove plate 101 for heating the deep hole plate by the heating module 2 is made of high-quality aluminum material, has good heat conducting property, can heat the nucleic acid extraction reagent in the deep hole plate, and meets the requirements of different stages of nucleic acid extraction on temperature.
Further, the upper silica gel plate 104 has two pieces and they are separately arranged for heating different areas of the well plate 101; the lower silicone plate 107 has two pieces and they are also separately disposed to facilitate their placement and replacement.
Further, the heating module 2 further includes a heat insulation board 106, the heat insulation board 106 is made of a heat insulation material, and the heat insulation board 106 is fixedly sleeved on the perforated slot board 101 and fixedly installed on the workbench 1, so as to insulate the perforated slot board 101 and the workbench 1.
Further, the nucleic acid extractor further comprises a protective cover shell and an air self-cleaning system, wherein the protective cover shell is arranged on the workbench 1, the air self-cleaning system comprises an ultraviolet sterilizing lamp 201, an exhaust fan 203 and a filter element 204 which are arranged on the protective cover shell, a fresh air inlet hole is further formed in the workbench 1, and the filter element 204 is arranged at an air outlet of the protective cover shell so as to filter air exhausted by the exhaust fan 203. When the instrument is in operation, the exhaust fan 203 exhausts air to the outside, the position of the fresh air inlet hole forms a negative pressure difference, the exhaust position of the exhaust fan 203 forms a positive pressure difference, air and aerosol in the anti-drop housing can not be discharged from the air inlet hole, and when the air is discharged outwards through the exhaust fan 203, a filter element 204 with high filtering precision filters out some substances which are possibly harmful; the ultraviolet sterilization lamp 201 can kill harmful substances possibly remaining in the reagent dropped on the table 1 when the apparatus is operated. The ultraviolet sterilizing lamp 201 and the air self-cleaning system can be operated safely and reliably continuously.
Further, the nucleic acid extraction instrument further comprises a support and a support driving mechanism, wherein the support driving mechanism comprises a support driving motor 53 and a belt transmission mechanism 55, the support driving motor 53 is connected with the support through the belt transmission mechanism 55 so as to be used for driving the support to horizontally move, and the magnetic rod sleeve module and the magnetic rod module are installed on the support. The support is installed on the workbench 1 through a horizontal first guide rail, a vertical second guide rail 16 is installed on the support, and the magnetic rod sleeve and the magnetic rod which are matched for use are respectively and preferably installed on the second guide rail 16 in a sliding mode through a sliding block. The support includes roof 35, curb plate 40 and crossbeam 51, roof 35 level setting, and the bottom surface of roof 35 is connected to the top surface of curb plate 40 and curb plate 40 is fixed in on the roof 35 through angle brace 50 to the vertical setting of curb plate 40, and the curb plate 40 is connected with crossbeam 51, and curb plate 40 is high-quality aluminum alloy material. The support is provided with a plurality of limit sensors 37, so that the limit sensors 37 are arranged at the limit positions of the support, the magnetic rod sleeve and the magnetic rod, and the limit positions of the support, the magnetic rod sleeve and the magnetic rod are used for limiting the moving stroke of the magnetic rod sleeve, the magnetic rod and the support, so that the problems that the movement cannot derail, collide with a machine and the like can be solved, and the movement in a safe range can be ensured during the movement.
The nucleic acid extractor is one integrated automatic nucleic acid preparing system and may be used in purifying nucleic acid from various biological samples, including whole blood, virus, tissue, plant, bacteria, cultured cell, etc. By means of an intelligent pre-installed extraction program, a matched nucleic acid extraction kit based on biological magnetic beads and consumable materials, the system can provide a high-efficiency, automatic and high-quality nucleic acid purification solution for a laboratory and is used for downstream gene analysis and molecular diagnosis.
It will be readily appreciated by those skilled in the art that the foregoing description is merely a preferred embodiment of the utility model and is not intended to limit the utility model, but any modifications, equivalents, improvements or alternatives falling within the spirit and principles of the utility model are intended to be included within the scope of the utility model.

Claims (9)

1. The utility model provides a multistation nucleic acid draws appearance, includes the workstation and sets up bar magnet cover module, bar magnet module and the heating module on the workstation, be provided with a plurality of station grooves on the workstation, at least one the station inslot sets up heating module, its characterized in that:
the heating module comprises a fixing plate, a hole groove plate, a heating block, an upper silica gel plate, a lower silica gel plate, a heat insulation plate and a temperature measuring circuit, wherein the heat insulation plate is made of heat insulation materials, the upper silica gel plate is made of heat conduction silica gel, the lower silica gel plate is made of heat insulation silica gel, the hole groove plate is provided with a plurality of deep hole pipes for accommodating deep hole plates, groove walls are in contact with the deep hole pipes, the fixing plate is installed on the workbench, the fixing plate is connected with the lower silica gel plate, the lower silica gel plate is connected with the heating block, the heating block is connected with the upper silica gel plate in an electric heating mode, the upper silica gel plate is connected with the hole groove plate in a surface contact mode, the heat insulation plate is fixedly sleeved on the hole groove plate and fixedly installed on the workbench, and the hole groove plate, the heat insulation plate and the fixing plate are respectively in sealing fit with the groove walls of the station groove, and the temperature measuring circuit is connected with the bottom surface of the hole groove plate in a sealing mode for detecting the temperature of the hole groove plate.
2. The apparatus of claim 1, wherein the upper silica gel plate has two pieces and is separately disposed for heating different areas of the well plate;
the lower silica gel plate has two pieces and they are also arranged separately.
3. The apparatus of claim 1, wherein the station well is provided with four stations.
4. The apparatus of claim 1, wherein the plate and the well plate are each made of an aluminum alloy.
5. The multi-station nucleic acid extractor of claim 1 further comprising a protective housing and an air self-cleaning system, wherein the protective housing is mounted on the table, the air self-cleaning system comprises an ultraviolet sterilizing lamp, an exhaust fan and a filter element mounted on the protective housing, the table is further provided with a fresh air inlet, and the filter element is mounted at an air outlet of the protective housing to filter air exhausted by the exhaust fan.
6. The multi-station nucleic acid extraction apparatus according to claim 1, wherein the magnetic rod sleeve module comprises a magnetic rod sleeve and a magnetic rod sleeve driving mechanism mounted on the workbench, the magnetic rod sleeve driving mechanism comprises a magnetic rod sleeve driving motor and a belt transmission mechanism, and the magnetic rod sleeve driving motor is connected with the magnetic rod sleeve through the belt transmission mechanism so as to drive the magnetic rod sleeve to move up and down.
7. The multi-station nucleic acid extraction apparatus according to claim 1, wherein the magnetic rod module comprises a magnetic rod and a magnetic rod driving mechanism mounted on the workbench, the magnetic rod driving mechanism comprises a magnetic rod driving motor and a screw mechanism, and the magnetic rod driving motor is connected with the magnetic rod through the screw mechanism for driving the magnetic rod to move up and down.
8. The multi-station nucleic acid extractor of claim 1 further comprising a rack and a rack drive mechanism, wherein the rack drive mechanism comprises a rack drive motor and a belt drive mechanism, the rack drive motor is connected with the rack through the belt drive mechanism for driving the rack to move horizontally, and the rack is provided with the magnetic rod sleeve module and the magnetic rod module.
9. The multi-station nucleic acid extractor of claim 8 wherein the support is provided with a plurality of limit sensors for limiting the travel of the support, the bars of the bar magnet module and the bar magnet housing of the bar magnet housing module.
CN202223142071.4U 2022-11-25 2022-11-25 Multi-station nucleic acid extractor Active CN219059002U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202223142071.4U CN219059002U (en) 2022-11-25 2022-11-25 Multi-station nucleic acid extractor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202223142071.4U CN219059002U (en) 2022-11-25 2022-11-25 Multi-station nucleic acid extractor

Publications (1)

Publication Number Publication Date
CN219059002U true CN219059002U (en) 2023-05-23

Family

ID=86346360

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202223142071.4U Active CN219059002U (en) 2022-11-25 2022-11-25 Multi-station nucleic acid extractor

Country Status (1)

Country Link
CN (1) CN219059002U (en)

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Address after: 18th Floor, Building A, No. 666 Shendun Fourth Road, Donghu New Technology Development Zone, Wuhan City, Hubei Province, 430200 (Wuhan Area of Free Trade Zone)

Patentee after: WUHAN NACI BIOTECHNOLOGY Co.,Ltd.

Address before: 430070 c201-202, 3rd floor, building B1, Guanggu biological city, Hongshan District, Wuhan City, Hubei Province

Patentee before: WUHAN NACI BIOTECHNOLOGY Co.,Ltd.

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