CN220450199U - PCR nucleic acid detection system - Google Patents

Abstract

The utility model belongs to the technical field of molecular diagnosis, and particularly discloses a PCR (polymerase chain reaction) nucleic acid detection system which comprises a clamping module, an extraction module, a liquid distribution module, a PCR amplification module and an ultraviolet lamp module, wherein the clamping module is used for clamping and fixing a sampling tube; the extraction module is used for extracting nucleic acid; the liquid preparation module is used for mixing the sample and the reagent; the ultraviolet lamp module is used for curing the sealant; the PCR amplification module is used for PCR amplification and detection; the reaction carrier of the PCR amplification module is a multichannel microfluidic chip. The PCR nucleic acid detection system provided by the utility model reduces the equipment volume, the detection cost and the detection time, the full-automatic integrated system for detecting the nucleic acid, which is applicable to a rapid detection scene, is realized.

Description

PCR nucleic acid detection system

Technical Field

The utility model belongs to the technical field of molecular diagnosis, and particularly relates to a PCR (polymerase chain reaction) nucleic acid detection system.

Background

Molecular biology techniques are used for gene screening or diagnosis, and mainly comprise techniques of molecular hybridization, fluorescence qualitative/quantitative, sequencing and the like, and the techniques mainly comprise the operation processes of sample nucleic acid extraction, PCR reaction system preparation, PCR detection and the like. The current rapid increase in sample size creates significant pressure and challenges for molecular detection. The introduction of an automatic molecular detection platform can certainly release the pressure of detection personnel to a great extent, and experimental errors such as artificial yin and artificial yang are avoided. At the same time, standardized mechanical operation can lead to shorter running time of the whole detection and more stable result. The existing full-automatic integrated equipment for detecting nucleic acid is large in size, high in liquid preparation cost, long in detection time, low in automation degree and not suitable for a scene of rapid detection.

Disclosure of Invention

The utility model aims to provide a PCR nucleic acid detection system for solving the technical problems.

The technical scheme provided by the utility model is as follows: the PCR nucleic acid detection system comprises a clamping module, an extraction module, a liquid preparation module, a PCR amplification module and an ultraviolet lamp module, wherein the clamping module is used for clamping and fixing a sampling tube; the extraction module is used for extracting nucleic acid; the liquid preparation module is used for mixing the sample and the reagent to prepare liquid after extraction; the ultraviolet lamp module is used for curing the sealant; the PCR amplification module is used for PCR amplification and detection; reaction carrier of PCR amplification module is a multichannel microfluidic chip; the PCR amplification module is arranged between the clamping module and the ultraviolet lamp module;

the multichannel microfluidic chip comprises a liquid injection hole, a gas outlet hole, a plurality of reaction cavities and a communication cavity sealed by sealant, wherein two ends of each reaction cavity are respectively provided with a sample injection end and a gas outlet end, the gas outlet ends and the reaction cavities form a U-shaped structure in a return bend arrangement mode, each sample injection end is connected with the communication cavity arranged at one end of the multichannel microfluidic chip through the liquid injection hole with a trapezoid longitudinal section, and each gas outlet end is connected with the communication cavity through the gas outlet hole arranged at the tail end of the gas outlet end.

Preferably, the extraction module comprises a magnetic rod and a magnetic rod sleeve sleeved outside the magnetic rod, and the magnetic rod has magnetic force when electrified; the liquid preparation module comprises a single tube or multiple tube groove for placing a single tube or multiple tubes with pre-buried reagents, and a sealant groove for placing sealant.

Preferably, the taper of the filling hole is 0.9 (+ -0.1): 2.7 (+ -0.1), and the aperture of the filling hole 801 is 1.8+ -0.2 mm.

Preferably, the gun head module comprises a limit base and a limit piece for limiting the gun head box.

Preferably, the mechanical arm module is used for opening and closing the cover of the sampling tube and loading and unloading the needle, and comprises a needle loading piece, an unloading piece and a gripper module.

Preferably, the sampling tube comprises a sampling tube seat, and the sampling tube seat is provided with a sampling tube groove which is opened upwards and used for placing a sampling tube.

Preferably, the ultraviolet lamp module comprises a rotating arm, an ultraviolet lamp, a connecting shaft and a rotating seat, wherein the rotating seat is fixedly connected to an operating platform of the detection box, the rotating arm rotates on the rotating seat through the connecting shaft, and the ultraviolet lamp is embedded in the bottom of the rotating arm and faces downwards.

Preferably, the device further comprises a detection box and a filtering module, wherein a clamping module, an extraction module, a liquid preparation module and a PCR amplification module are arranged in the detection box, and the filtering module is fixed at the top of the detection box.

Preferably, the device further comprises a gun head waste collection tank for collecting waste and a waste needle drawer for collecting waste, wherein the detection tank comprises a base and an operation table, the waste collection tank is arranged on the operation table, a slot is formed in the base and used for installing the waste needle drawer, and the waste needle drawer is arranged below the waste collection tank and is communicated with the waste needle drawer.

The utility model has the beneficial effects that:

1. the PCR nucleic acid detection system provided by the utility model integrates the processes of clamping, liquid preparation, nucleic acid extraction and nucleic acid amplification detection, has small equipment volume and low detection cost, and aims at on-site and rapid detection, thereby greatly reducing the cost of reagent detection; compared with the existing detection system which needs 2 hours to finish detection, the PCR nucleic acid detection system provided by the utility model can realize rapid and convenient detection from 'sample in' to 'result out' in 45 minutes.

2. The extraction module in the PCR nucleic acid detection system provided by the utility model is characterized in that a magnetic rod sleeve is sleeved outside the magnetic rod, the magnetic rod sleeve moves up and down to vibrate and mix the liquid in the deep hole plate uniformly; the single tube or multiple tube slot in the PCR nucleic acid detection system provided by the utility model is used for placing the single tube or multiple tubes with pre-buried reagents, the micro-reaction system is arranged in the single tube or the multi-connected tube, and is uniformly mixed by the gun head, so that no bubbles are generated during uniform mixing.

3. The multichannel microfluidic chip has a unique structure, a PCR reaction system is injected into the liquid injection hole, air in the reaction cavity is discharged through the air outlet hole in the liquid injection process, the liquid injection hole is of a truncated cone structure with a wide upper part and a narrow lower part, and the aperture with a wide upper part can increase the fault tolerance to the positioning of the mechanical arm module. Even the deviation in 0.5mm takes place when the arm module is fixed a position, the rifle head still can slide to the bottom of annotating the liquid hole along wide hole site to the rifle head is laminated with annotating liquid Kong Yanmi, avoids annotating the pressure and leaks when liquid, and liquid emits.

After liquid injection, the multichannel microfluidic chip is injected with sealant at the communication cavity for sealing, and the sealing mode of sealing the communication cavity through the sealant is unique. The liquid injection hole and the air outlet hole of the multichannel microfluidic chip adopt a mode that the U-shaped opening is upward, so that on one hand, sample liquid is prevented from flowing downwards to leak under the gravity condition, and on the other hand, the liquid injection hole and the air outlet hole of the multichannel microfluidic chip are positioned on the same plane, so that the sealant can be conveniently smeared and sealed. For the multichannel micro-fluidic chip, the sealing glue mode is easy to realize, and a set of mechanical arm module and gun head consumable can be shared, so that the volume of the machine is reduced.

4. The detection time of the nucleic acid extraction and detection integrated machine mainly depends on the time of nucleic acid extraction and nucleic acid amplification detection, wherein the nucleic acid extraction mainly depends on reagents and has little relation with hardware. Therefore, the utility model improves the efficiency of nucleic acid amplification detection, and based on the ultra-fast PCR amplification detection module, the total detection time from the sample to the result is completed within 45 minutes.

5. The PCR nucleic acid detection system provided by the utility model reduces the equipment volume, the detection cost and the detection time, and realizes a full-automatic integrated system for nucleic acid detection, which is applicable to a rapid detection scene.

Drawings

FIG. 1 is a top view of the present utility model;

FIG. 2 is a front view of the present utility model;

FIG. 3 is a schematic view of a sampling tube module of the present utility model;

FIG. 4 is a schematic view of a clamping module according to the present utility model;

FIG. 5 is a schematic diagram of an extraction module according to the present utility model;

FIG. 6 is a schematic diagram of a liquid dispensing module according to the present utility model;

FIG. 7 is a schematic view of a gun head module according to the present utility model;

FIG. 8 is a schematic diagram of a PCR amplification module according to the present utility model;

FIG. 9 is a schematic diagram of a multi-channel microfluidic chip according to the present utility model;

FIG. 10 is a schematic diagram of an ultraviolet lamp module according to the present utility model;

FIG. 11 is a schematic diagram of a robot arm module according to the present utility model;

FIG. 12 is a detection flow of the present utility model.

Reference numerals: sampling tube module 1, sampling tube holder 101, sampling tube slot 102, clamping module 2, clamping box 201, fixing block 202, moving block 203, driving piece 204, extraction module 3, magnetic rod 301, magnetic sleeve rod 302, deep hole plate 303, heating module 304, liquid preparation module 4, liquid preparation seat 401, single tube or multi-tube slot 402, sealant slot 403, gun head module 6, projection 601, limit base 602, limit plate 603, ball 604, waste collection slot 7, PCR amplification module 8, liquid injection hole 801, gas outlet 802, reaction cavity 803, communication cavity 804, ultraviolet lamp module 9, rotating arm 901, ultraviolet lamp 902, connecting shaft, rotating seat 904, filter module 10, mechanical arm module 11, needle loading piece 1101, needle removing piece 1102, gripper module 1103, code scanning module 1104, waste needle drawer 12, detection box 13, base 1301, console 1302, single tube or multi-tube 15, sealant 16, gun head 17.

Detailed Description

The following is a further detailed description of the embodiments:

as shown in fig. 1 to 12, the present embodiment provides a PCR nucleic acid detection system, which includes a detection box 13, a sampling tube module 1, a clamping module 2, an extraction module 3, a liquid preparation module 4, a gun head module 6, a PCR amplification module 8, an ultraviolet lamp module 9, a filter module 10, a mechanical arm module 11, a waste collection tank 7, and a waste needle drawer 12.

Fig. 1 is a top view of the present utility model, fig. 2 is a front view of the present utility model, and as shown in fig. 1 and 2, in this embodiment, the detection box 13 includes a base 1301 and an operation table 1302, on the operation table 1302, the waste collection tank 7 is elongated in a top view, the sampling tube module 1 and the clamping module 2 are disposed on one side of the waste collection tank 7, and the gun head module 6 is disposed on the other side of the waste collection tank 7; the PCR amplification module 8 is arranged close to the sampling tube module 1 and between the clamping module 2 and the ultraviolet lamp module 9, the liquid preparation module 4 is arranged close to the gun head module 6, and the extraction module 3 is arranged close to the liquid preparation module 4.

(1) With respect to the sampling tube module 1

FIG. 3 is a schematic view of a sampling tube module according to the present utility model. As shown in fig. 3, the sampling tube module 1 includes a sampling tube holder 101, and a sampling tube slot 102 opened upward is provided on the sampling tube holder 101, and the sampling tube slot 102 is used for placing the sampling tube 14. In this embodiment, 8 sampling tube slots 102 are integrally connected to the sampling tube holder 101, so that 8 sampling tubes 14 with different specifications can be compatible.

(2) With respect to the clamping module 2

Fig. 4 is a schematic view of the clamping module of the present utility model, as shown in fig. 4, the clamping module 2 is used to clamp and fix the sampling tube 14, so as to facilitate the mechanical arm module 11 to rotate the sampling tube 14 to open or close the cover. As shown in fig. 4, the clamping module 2 includes a clamping case 201, a fixed block 202, a moving block 203, and a driving member 204.

A clamping groove which is opened upward is provided in the clamping box 201, and a fixed block 202 and a moving block 203 which can approach or separate from the fixed block 202 are arranged in the clamping groove. The driving piece 204 is used for driving the moving block 203 to approach or separate from the fixed block 202. In this embodiment, the driving member 204 includes a clamping motor, and a transmission shaft is connected to an output shaft of the clamping motor. The moving block 203 is matched with the clamping groove, and a transmission shaft is in threaded connection with the moving block 203 to drive the moving block 203 to move only along the direction of the output shaft of the clamping motor.

When the sampling tube 14 is placed in the clamping groove in operation, the clamping motor is started, the output shaft and the transmission shaft of the clamping motor rotate, and the transmission shaft drives the moving block 203 to approach or separate from the fixed block 202, so that the action of clamping or loosening the sampling tube 14 is realized.

(3) With respect to the extraction module 3

Fig. 5 is a schematic diagram of an extraction module in the present utility model, wherein the extraction module 3 is designed based on a magnetic bead method, and is used for extracting nucleic acid, and as shown in fig. 5, the extraction module 3 comprises a magnetic rod 301, a magnetic rod sleeve 302, a deep hole plate 303, a heating module 304 and an extraction base. Wherein, magnetic rod sleeve 302 and deep hole plate 303 are disposable consumables. The magnetic rod 301 and the magnetic rod sleeve 302 can move along the x axis (the extraction base is integrally connected with a sliding rail) and the z axis in fig. 5 (the magnetic rod sleeve 302 is sleeved outside the magnetic rod 301, and the magnetic rod sleeve 302 moves up and down to vibrate and mix the liquid in the deep hole plate 303); the magnetic rod 301 has magnetic force when being electrified and does not have magnetic force when being powered off, and the magnetic force state of the magnetic rod 301 is switched by the on-off state, so that the adsorption transfer of the magnetic beads is realized. The magnetic rod 301 is used for providing magnetic force, and the magnetic rod sleeve 302 is sleeved on the magnetic rod 301 when in adsorption and transfer, and the magnetic beads are adsorbed on the magnetic rod sleeve 302 by the attraction of the magnetic rod 301. The heating module 304 heats the liquid in the deep-hole plate 303 to meet the extracted temperature condition requirement.

(4) With respect to the liquid dispensing module 4

Fig. 6 is a schematic diagram of a liquid dispensing module according to the present utility model, and the liquid dispensing module 4 includes a liquid dispensing seat 401, and 2 rows of single-tube or multi-tube slots 402 and 2 sealant slots 403 disposed on the liquid dispensing seat 401. The single tube or multi-tube slot 402 is used for placing a single tube or multi-tube 15 with pre-buried reagents, in this embodiment, the multi-tube is taken as an example, and a micro-reaction system is arranged in the multi-tube, and the micro-reaction system is uniformly mixed by a gun head, so that no bubbles are generated during uniform mixing. The sealant groove 403 is used for placing the sealant 16.

In other alternative embodiments, the manner in which the gun head applies the sealant may be replaced with a dedicated dispensing pump. The sealant 16 may be replaced with other curable liquids.

(5) Gun head module 6

Fig. 7 is a schematic view of a gun head module according to the present utility model. As shown in fig. 7, the gun head module comprises a gun head box, a limiting base 602 and a limiting piece for limiting the gun head box. The gun head box can be matched with 1000 mu l gun heads, 200 mu l gun heads and/or 50 mu l gun heads with other specifications for use, and the bottom side surface of the gun head box is integrally connected with a protrusion 601. In this embodiment, the limiting member at least includes a set of limiting plates 603 arranged opposite to each other, the limiting base 602 is a cuboid, and four sides of the limiting base 602 are respectively and fixedly connected with a limiting plate 603. The limiting plate 603 is provided with a limiting hole, a spring and a ball 604 are accommodated in the limiting hole, the diameter of the ball 604 is larger than that of the limiting hole, one end of the spring is connected with the inner side wall of the limiting plate 603, and the other end of the spring is connected with the ball 604, and in the embodiment, the connection mode is bonding.

When the empty box is in a state, the round beads 604 protrude from the limiting holes under the action of the springs, when the gun head box is pressed down, the round beads 604 move into the limiting plates 603/the limiting holes under the action of the pressure of the gun head box protrusions 601, when the gun head box is placed at bottom, the gun head box protrusions 601 move to the lower sides of the round beads 604, the round beads 604 clamp the gun head box, and the gun head box is fixed on the limiting base 602. The limiting mode adopted in the embodiment is a soft limiting mode, and the gun head box can be lifted by manually slightly and forcefully lifting the gun head box. In other alternative embodiments, the method may be changed to a hard limit method, for example, locking by using a buckle, and the gun head box can be taken out only by canceling the limit.

In other alternative embodiments, the limiting plate 603 is provided with an elastic clamping block capable of fixing the gun head box.

(6) PCR amplification Module 8

The PCR amplification module 8 is arranged between the clamping module 2 and the ultraviolet lamp module 9, and the PCR amplification module 8 is used for PCR amplification and detection. FIG. 8 is a schematic diagram of a PCR amplification module according to the present utility model, where the PCR amplification module 8 includes a multichannel microfluidic chip, a main control module, a temperature control module, and a photo-detection module, as shown in FIG. 8.

The main control module sends out specific instructions to drive other modules to operate, and receives feedback of each module to perform necessary real-time processing. The temperature control module is responsible for raising and lowering the temperature of the microfluidic chip and guaranteeing the temperature conditions required by the PCR amplification of the sample in the microfluidic chip. In the PCR amplification process, the photoelectric detection module excites fluorescent signals of a sample, collects and records the fluorescent signals, the data are fed back to the central control module for data analysis, and finally a detection result is obtained through algorithm judgment.

The multichannel microfluidic chip is a reaction carrier. Fig. 9 is a schematic diagram of a multichannel microfluidic chip according to the present utility model. As shown in fig. 9, the multi-channel microfluidic chip in this embodiment is preferably a 4-channel microfluidic chip, and the 4-channel microfluidic chip includes a liquid injection hole 801, an air outlet hole 802, a plurality of reaction chambers 803, and a communication chamber 804. The 4-channel microfluidic chip has a thickness range of 1+ -0.1 mm, a width range of 14.5+ -1 mm, and a length of the reaction chamber 803 of 28+ -1 mm. In this embodiment, one end of the reaction cavity 803 is a sample injection end arranged downwards, the other end of the reaction cavity 803 is an air outlet end arranged upwards and backwards, each sample injection end is connected with a communicating cavity 804 arranged at one end of the multichannel microfluidic chip through a liquid injection hole 80 with a trapezoid longitudinal section, and each air outlet end is connected with the communicating cavity 804 through an air outlet hole 802 arranged at the tail end of the air outlet end, the taper of the liquid injection hole 801 is 0.9 (+ -0.1): 2.7 (+ -0.1), and the aperture of the liquid injection hole 801 is 1.8+ -0.2 mm. The outlet end is provided with an outlet hole 802 with a pore diameter range of 0.5+/-0.1 mm.

During operation, the PCR reaction system is injected into the liquid injection hole 801 through the gun head, and in the liquid injection process, the air in the reaction cavity 803 is discharged through the air outlet hole 802. The reaction chamber has a volume of about 12. Mu.l. The liquid injection hole 801 is of a round platform structure with a wide upper part and a narrow lower part, and the aperture with a wide upper part can increase the fault tolerance for positioning the mechanical arm module and can be matched with the gun head better. Even if the deviation within 0.5mm occurs during the positioning of the mechanical arm module, the gun head can still slide to the bottom of the liquid injection hole 801 along the wide hole position, and the gun head is tightly attached to the liquid injection hole 801, so that the pressure leakage and the liquid discharge during liquid injection are avoided.

In sealing, the sealant 16 is injected into the communication chamber 804 to seal.

(7) Ultraviolet lamp module 9

The ultraviolet lamp module 9 is used for curing the sealant 16. FIG. 10 is a schematic diagram of an ultraviolet lamp module according to the present utility model; as shown in fig. 10, the ultraviolet lamp module 9 includes a rotating arm 901, an ultraviolet lamp 902, a connecting shaft 903, and a rotating base 904, the rotating base 904 is fixedly connected to an operation table 1302 of the detection box 13 by bolts, the rotating arm 901 rotates on the rotating base 904 by the connecting shaft 903, and the ultraviolet lamp 902 is embedded in the bottom of the rotating arm 901 and the irradiation surface faces downward. When in operation, under the drive of the connecting shaft 903, the rotating arm 901 rotates to a proper position, then the ultraviolet lamp 902 is turned on, and the irradiation is reset after completion. Because the ultraviolet lamp 902 is high in power, in order to protect other parts and users, the rotating arm 901 is designed to be made of opaque metal materials, and plays a role in shading light.

(8) Filter module 10

The filter module 10 is fixed on the top of the detection box 13 by bolts, and needs to keep running in the whole operation process of the detection system, so as to guide the air flow in the detection box 13 to finally reach the filter module 10 from the extraction module 3 through the PCR amplification module, and prevent the air flow diffusion in a possibly polluted area.

In this embodiment, the filter module 10 is a fan, and in other alternative embodiments, the filter module 10 uses a fan plus HEPA (high efficiency particulate), so that the air can be filtered by the filter screen to adsorb the particulate in addition to preventing the air flow in the area where the pollution may occur from diffusing, and the pollution is reduced.

(9) Mechanical arm module 11

The mechanical arm module 11 is used for opening and closing the cover of the sampling tube 14, and loading and unloading the needle. The robotic arm module 11 includes a single channel pipetting module, a gripper module 1103 and a code scanning module 1104. The single channel pipetting module consists of needle mount 1101 and needle release 1102.

Fig. 11 is a schematic view of a robot module according to the present utility model. As shown in FIG. 11, needle stripper 1102 is nested outside of needle holder 1101. Needle holder 1101 is depressed to load gun 17, securing gun 17 to needle holder 1101. The needle removing member 1102 is driven by a motor and is movable downward, and when moved downward, the needle removing member is moved downward by a pushing force.

The gripper module 1103 realizes a gripping function by controlling the opening and closing amplitude, and simultaneously can rotate to realize a cover opening and closing function.

The code scanning module 1104 is arranged at the rear of the gripper module 1103, when the gripper module 1103 grabs the sampling tube 14, the code scanning module 1104 just faces the bottle body of the sampling tube 14, the gripper module 1103 rotates with the sampling tube 14, and the rotating code scanning module 1104 keeps a continuous scanning state until the code scanning module 1104 aims at the two-dimensional code of the bottle body of the sampling tube 14, so that the scanning is successful.

(10) Waste collection tank 7 and waste needle drawer 12

A waste collection tank 7 is disposed on the console 1302, the waste collection tank 7 opening upward.

The base 1301 is internally slotted for mounting a waste needle drawer 12, the waste needle drawer 12 can be manually withdrawn and cleaned of waste, the waste needle drawer 12 is below the waste collection tank 7 and the waste collection tank 7 is in communication with the waste needle drawer 12. The waste is mainly the waste gun head, and when the waste gun head is discarded, the waste gun head falls into the waste needle drawer 12 through the waste collecting tank 7.

(11) Experimental procedure of PCR detection System provided in this embodiment

FIG. 12 is a detection flow of the present utility model. As shown in fig. 12, the method specifically comprises the following steps:

s1, placing consumable materials by a user: consumable materials include sampling tube 14, tip box (including 1000. Mu.l tip box, 200. Mu.l/50. Mu.l tip box), multichannel microfluidic chip, sealant 16, multiplex tube 15, deep well plate 303, etc.

S2, code scanning and sampling:

(1) The robotic arm module 11 grabs the sampling tube 14 and the code scanning module 1104 identifies sample information on the sampling tube 14.

(2) After the sampling tube 14 is placed on the clamping groove in the clamping box 201, the mechanical arm module 11 performs rotary uncovering on the sampling tube 14 after the clamping module 2 fixes the bottle body of the sampling tube 14.

(3) The mechanical arm module 11 inserts the gun head 17 and moves the liquid in the sampling tube 14 into the deep hole plate 303 of the extraction module 3.

(4) The robotic arm module 11 withdraws the gun head 17 into the needle waste drawer 12 (this action is performed after each pipetting, which will not be described later), and the robotic arm module 11 rotates the cap for the sampling tube 14. The robotic arm module 11 replaces the sampling tube 14 within the sampling tube slot 102.

S3, extracting: and executing an extraction program to finally obtain an extracted nucleic acid sample.

S4, preparing a reaction system liquid: the mechanical arm module 11 inserts the gun head 17, and the deep hole plate 303 in the extraction module 3 is pipetted into the multi-connected pipe 15 in the liquid preparation module 4 and uniformly mixed with the pre-buried reagent in the multi-connected pipe 15 to obtain a reaction system. In the mixing process, in order to minimize the generation of bubbles, the mixing operation is performed by sucking and discharging the liquid a plurality of times while keeping the liquid below the liquid surface, and the speed of sucking and discharging is adjusted to be slow.

If bubbles are generated in this step, the liquid absorption in the subsequent step may be inaccurate, thereby affecting the experimental result.

S5, injecting liquid into the reaction system: the mechanical arm module 11 is inserted into the gun head 17, and is pipetted into the multichannel microfluidic chip of the PCR amplification module 8 from the multi-connected tube 15. In the process of injecting liquid, the gun head 17 needs to be ensured to be pressed against the liquid injection hole 801, and tight fitting is the key of liquid injection.

S6, sealing the reaction piece:

(1) The mechanical arm module 11 is inserted into the gun head 17, and is pipetted into the communication cavity 804 of the multichannel microfluidic chip of the PCR amplification module 8 from the sealant 16 of the liquid preparation module 4.

(2) The ultraviolet lamp module 9 rotates to the position above the multichannel microfluidic chip, the ultraviolet lamp 902 is turned on, the curing of the sealant 16 is completed after 3 seconds, and the ultraviolet lamp module 9 is reset.

S7, PCR amplification detection: in the PCR amplification module 8, PCR amplification and detection are completed, and a detection report is provided.

(12) Verification experiment

1. The experimental protocol was as follows:

the PCR nucleic acid provided by the utility model the detection system is experiment group 1;

a full-automatic nucleic acid extraction and purification instrument (nucleic acid extraction) +ABI 7500 real-time fluorescence quantitative PCR instrument (nucleic acid amplification detection) of the Bo GenePure Pro 32E is used as a control group 2;

nucleic acid extraction: positive quality control of monkey pox virus nucleic acid detection kit + nucleic acid extraction kit;

nucleic acid detection: PCR reaction liquid of a monkey pox virus nucleic acid detection kit;

2. the test group 1 and the control group 2 were subjected to nucleic acid extraction and nucleic acid amplification using the same sample and reagent, and the detection results of the two groups were compared, and specific detection results are shown in tables 1 and 2 below:

table 1 test results of experimental group 1 and control group 2

Table 2 comparison of detection times for experimental group 1 and control group 2

From the experimental results, it can be demonstrated that: the PCR nucleic acid detection system provided by the utility model can complete rapid and convenient detection within 45 minutes, and the detection result is consistent with the detection effect of the existing mature instrument, and the function and performance verification is feasible.

The foregoing describes in detail preferred embodiments of the present utility model. It should be understood that numerous modifications and variations can be made in accordance with the concepts of the utility model by one of ordinary skill in the art without undue burden. Therefore, all technical solutions which can be obtained by logic analysis, reasoning or limited experiments based on the prior art by the person skilled in the art according to the inventive concept shall be within the scope of protection defined by the claims.

Claims (9)

1. The PCR nucleic acid detection system is characterized by comprising a clamping module (2), an extraction module (3), a liquid preparation module (4), a PCR amplification module (8) and an ultraviolet lamp module (9), wherein the clamping module (2) is used for clamping and fixing a sampling tube (14); extraction module (3) for extracting nucleic acids; the liquid preparation module (4) is used for mixing the sample and the reagent; the ultraviolet lamp module (9) is used for curing the sealant (16); the PCR amplification module (8) is used for PCR amplification and detection; the reaction carrier of the PCR amplification module (8) is a multichannel microfluidic chip; the PCR amplification module (8) is arranged between the clamping module (2) and the ultraviolet lamp module (9);

the multichannel microfluidic chip comprises a liquid injection hole (801), a gas outlet hole (802), a plurality of reaction cavities (803) and a communication cavity (804) sealed by sealant (16), wherein two ends of each reaction cavity (803) are respectively provided with a sample injection end and a gas outlet end, the gas outlet ends and the reaction cavities (803) form a U-shaped structure in a return bend arrangement mode, each sample injection end is connected with the communication cavity (804) arranged at one end of the multichannel microfluidic chip through the liquid injection hole (801) with a trapezoid longitudinal section, and each gas outlet end is connected with the communication cavity (804) through the gas outlet hole (802) arranged at the tail end of the gas outlet end.

2. The PCR nucleic acid detection system according to claim 1, wherein the extraction module (3) comprises a magnetic rod (301) and a magnetic rod sleeve (302) sleeved outside the magnetic rod (301), and the magnetic rod (301) has magnetic force when energized; the liquid distribution module (4) comprises a single tube or multi-tube groove (402) for placing a single tube or multi-tube (15) with pre-buried reagents and a sealant groove (403) for placing sealant (16).

3. The PCR nucleic acid detecting system according to claim 1, wherein the taper of the liquid injection hole (801) is 0.9 (+ -0.1): 2.7 (+ -0.1), and the aperture of the liquid injection hole (801) is 1.8 (+ -0.2 mm).

4. The PCR nucleic acid detection system of claim 1, further comprising a gun head module (6), the gun head module (6) comprising a limit base (602) and a limit for limiting the gun head cartridge.

5. The PCR nucleic acid detection system in accordance with claim 1, further comprising a robotic arm module (11) for opening and closing the lid of the sampling tube (14), and for loading and unloading the needle, the robotic arm module (11) comprising a needle loading member (1101), an unloading member (1102) and a gripper module (1103).

6. The PCR nucleic acid detection system of claim 1, further comprising a sampling tube module (1), wherein the sampling tube module (1) comprises a sampling tube holder (101), and wherein the sampling tube holder (101) is provided with a sampling tube slot (102) which is opened upwards, and wherein the sampling tube slot (102) is used for placing a sampling tube (14).

7. The PCR nucleic acid detection system as claimed in claim 1, wherein the ultraviolet lamp module (9) includes a rotating arm (901), an ultraviolet lamp (902), a connection shaft (903) and a rotating base (904), the rotating base (904) is fixedly connected to an operation table (1302) of the detection box (13), the rotating arm (901) rotates on the rotating base (904) through the connection shaft (903), and the ultraviolet lamp (902) is disposed at the bottom of the rotating arm (901) with the irradiation face downward.

8. The PCR nucleic acid detection system according to claim 1, further comprising a detection box (13) and a filter module (10), wherein the clamping module (2), the extraction module (3), the liquid preparation module (4) and the PCR amplification module (8) are arranged in the detection box (13), and the filter module (10) is fixed at the top of the detection box (13).

9. The PCR nucleic acid detection system in accordance with claim 8, further comprising a gun head (17) waste collection tank (7) for collecting waste and a waste needle drawer (12) for collecting waste, the detection tank (13) comprising a base (1301) and an operation table (1302), the waste collection tank (7) being arranged on the operation table (1302), the base (1301) being internally slotted for mounting the waste needle drawer (12), the waste needle drawer (12) being located below the waste collection tank (7) and the waste collection tank (7) being in communication with the waste needle drawer (12).