CN117736833A

CN117736833A - Food sample pretreatment workstation

Info

Publication number: CN117736833A
Application number: CN202311460853.9A
Authority: CN
Inventors: 张裕祥; 杜美红; 李静雯
Original assignee: Analysis And Testing Institute Of Beijing Academy Of Science And Technology Beijing Physical And Chemical Analysis And Testing Center
Current assignee: Analysis And Testing Institute Of Beijing Academy Of Science And Technology Beijing Physical And Chemical Analysis And Testing Center
Priority date: 2022-11-04
Filing date: 2023-11-04
Publication date: 2024-03-22

Abstract

The invention provides a food sample pretreatment workstation which can realize high-throughput, automatic and rapid treatment of food samples. The food sample pretreatment workstation comprises: a homogenizing station, an incubation station and a separation station are arranged in the workstation; the homogenizing station is provided with a homogenizing module for homogenizing food samples and culture fluid; the homogenizing module adopts a cutting, stirring and uniformly mixing mode to realize homogenization; the incubation station is provided with an independent openable closed space and is used for placing an incubation module and performing incubation treatment in a set temperature environment; a separation module is arranged on the separation station, and the separation module adopts an immunomagnetic separation process to realize rapid enrichment and separation of target microorganisms; the transmission module is used for realizing the transfer of the sample tube among the stations.

Description

Food sample pretreatment workstation

Technical Field

The invention relates to a food sample pretreatment workstation, belonging to the microorganism detection technology.

Background

The food-borne pathogenic bacteria can cause symptoms such as fever, diarrhea, vomiting and the like, and are fatal in severe cases, so that the health of people is seriously endangered, and the food-borne pathogenic bacteria are the primary cause of food safety events in China every year. At present, hundreds of millions of food samples in China need to be detected for pathogenic bacteria every year, almost all the food samples depend on manual operation, and time and labor are wasted. Particularly, with the rising of takeaway economy and the holding of a large international conference, higher requirements are put on the timeliness of pathogenic bacteria detection, so that quick detection technologies such as qPCR, LAMP and the like are continuously developed. However, the food substrate is complex and has various components, and the pretreatment of the sample before detection is necessary, but at present, an automatic food sample pretreatment instrument suitable for microorganism detection is not available except for the step of DNA extraction.

Disclosure of Invention

In view of the above, the present invention provides a food sample pretreatment workstation, which can implement high-throughput, automatic and rapid treatment of food samples.

The food sample pretreatment workstation comprises: a homogenizing station, an incubation station and a separation station are arranged in the workstation;

the homogenizing station is provided with a homogenizing module for homogenizing food samples and culture fluid; the homogenizing module adopts a cutting, stirring and uniformly mixing mode to realize homogenization;

the incubation station is provided with an independent openable closed space and is used for placing an incubation module and performing incubation treatment in a set temperature environment;

a separation module is arranged on the separation station, and the separation module adopts an immunomagnetic separation process to realize rapid enrichment and separation of target microorganisms;

the transmission module is used for realizing the transfer of the sample tube among the stations.

As a preferred mode of the present invention: the internal space of the workstation is divided into an upper layer and a lower layer, the homogenizing station and the incubation station are arranged on the upper layer, and the separating station is arranged on the lower layer;

the transmission module includes: the device comprises a sample transfer mechanism A arranged on an upper layer, a sample transfer mechanism B arranged on a lower layer and a large pipe sample vertical transmission mechanism for connecting the upper layer and the lower layer; the sample transfer mechanism A is used for transferring the large tube at each position of the upper layer, the transfer mechanism B is used for transferring the large tube at each position of the lower layer, and the large tube sample vertical transfer mechanism is used for transferring the large tube in the upper layer to the lower layer;

The upper space of the workstation is divided into a left part and a right part, and a homogenizing station is arranged in the left space;

the right side of the upper space of the workstation is divided into a front part, a middle part and a rear part; the front side is provided with a station switching panel, and a large pipe storage position, a combined storage position and a large pipe incubation position which are sequentially arranged on the station switching panel along the transverse direction; the large tube incubation position is provided with a large tube incubation module; the combined storage position is provided with a liquid gun storage position A, a magnetic bead storage position and a pretreatment liquid storage position; the station switching panel can move back and forth to switch positions;

the middle part of the right side of the upper space is provided with a mounting plate, and the rear side surface of the mounting plate is provided with a large pipe switch cover mechanism, a pipetting mechanism A and a grabbing mechanism A in sequence along the transverse direction;

the big pipe switch cover mechanism is used for clamping a big pipe in the big pipe storage position, and placing the big pipe on the sample transfer mechanism A after the action of opening or closing the cover is executed; the pipetting mechanism A is used for picking up gun heads in the liquid gun storage position A, sucking immune magnetic bead reagents in the magnetic bead storage position or sucking pretreatment liquid in the pretreatment liquid storage position, and adding the immune magnetic bead reagents or the pretreatment liquid in the pretreatment liquid storage position into a large pipe; the grabbing mechanism A is used for grabbing the big pipe after incubation in the big pipe incubation position and placing the big pipe on the big pipe sample vertical transmission mechanism.

As a preferred mode of the present invention: the rear part of the right side of the upper space is also provided with a small pipe incubation position for placing a small pipe incubation module; the transmission module further comprises: and the small pipe sample vertical transmission mechanism is connected with the upper layer and the lower layer.

As a preferred mode of the present invention: the large pipe incubation position and the small pipe incubation position are sealed and isolated by an openable incubation bottom plate sealing structure.

As a preferred mode of the present invention: the whole lower space of the workstation is divided into a front part, a middle part and a rear part;

the front side is provided with a magnetic separation station switching bottom plate, a small pipe storage position, a small magnetic rod sleeve storage position, a treated sample storage position, a washing liquid storage position, a culture solution storage position, a large magnetic rod sleeve storage position and a middle pipe storage position which are sequentially arranged on the magnetic separation station switching bottom plate along the transverse direction; the front sides of the washing liquid storage position and the culture solution storage position are respectively provided with a liquid gun storage position B correspondingly, and gun heads for sucking washing liquid and culture solution are respectively stored;

the middle part of the lower space is provided with a mounting plate, and the rear side surface of the mounting plate is provided with a middle/small tube switch cover mechanism, a small magnetic rod separating assembly, two groups of pipetting mechanisms B, a large magnetic rod separating assembly and a grabbing mechanism B in sequence along the transverse direction.

As a preferred mode of the present invention: also comprises a biological safety module;

the biosafety module includes: the air duct A and the fan A are arranged in the upper space, the air duct B and the fan B are arranged in the lower space, the ultraviolet sterilizing lamp is arranged above the inside of the homogenizing module, and the ultraviolet sterilizing lamp is arranged at the side edge of the discarding station.

As a preferred mode of the present invention: the automatic control module is used for controlling the homogenizing module, the incubation module and the separation module to automatically operate according to preset working conditions and modes.

As a preferred mode of the present invention: the homogenizing module comprises: the device comprises a shell, a homogenizing tank support frame, a homogenizing tank assembly, a pressurizing assembly and a homogenizing driving assembly;

the homogenizing tank support frame is transversely supported inside the shell through a homogenizing module revolution shaft, and the homogenizing module revolution shaft can drive the homogenizing tank support frame to rotate around the axis of the homogenizing tank support frame;

the homogenizing tank support frames are uniformly distributed with a plurality of homogenizing tank assemblies at intervals along the circumferential direction, and each homogenizing tank assembly comprises a plurality of homogenizing tanks;

a pressurizing assembly is arranged on the shell above the homogenizing tank supporting frame, and when one of the homogenizing tank assemblies rotates below the pressurizing assembly, the pressurizing assembly downwardly presses the culture solution in the homogenizing tank assembly to discharge the culture solution from the homogenizing tank;

And a homogenizing driving assembly is arranged on the shell below the homogenizing tank supporting frame, and when one of the homogenizing tank assemblies rotates to the position above the driving assembly, the homogenizing driving assembly drives the blades in the homogenizing tank to rotate.

As a preferred mode of the present invention: the homogenizing tank assembly is supported on the homogenizing tank support frame through a homogenizing module rotating shaft; the homogenizing module rotating shaft can drive the homogenizing tank assembly to rotate around the axis of the homogenizing tank assembly to realize autorotation.

As a preferred mode of the present invention: the homogenizing tank includes: the device comprises a tank body, a piston head, a base and a blade;

the top opening of the tank body is coaxially provided with a piston head matched with the pressurizing assembly, and the piston head can downwards squeeze the culture solution in the tank body under the pushing of the pressurizing assembly;

the opening at the bottom of the tank body is sealed through a base; the upper end face of the base is connected with a blade through a rotating shaft; the rotating shaft extends out of the base and is detachably connected with the homogenizing driving assembly;

the lower end face of the base is provided with a filtrate port with a one-way valve.

The beneficial effects are that:

(1) The food sample pretreatment workstation integrates homogenization, incubation and separation into one device, and can realize high-throughput, automatic and rapid treatment of food samples.

(2) The food sample pretreatment workstation is reasonable in layout, and cross contamination among samples in the same batch in the running process of the instrument is eliminated by arranging the biological safety module.

(3) According to the food sample pretreatment workstation, the separation module adopts an immunomagnetic separation process to realize rapid enrichment and separation of target microorganisms.

(4) The food sample pretreatment workstation disclosed by the invention has the advantages that the incubation station is provided with the independent openable and closable closed space, so that the incubation treatment can be carried out in a set temperature environment.

(5) In the homogenizing module, a plurality of independent homogenizing tanks are integrated, different samples and culture solutions can be filled in each homogenizing tank, the plurality of homogenizing tanks can be synchronously processed, and further, the purposes of homogenizing processed various food samples at the same time and saving time are realized; and the pressurizing assembly and the homogenizing driving assembly are controlled, so that automation of the homogenizing process can be realized; and homogenizing the food sample according to a preset program.

(6) The homogenizing tank in the homogenizing module abandons the traditional beating type food sample homogenizing technology, adopts a cutting and stirring mode to enable the food sample to be fully contacted with the culture solution, has small impact and vibration in the treatment process, can realize automatic treatment, and can be embedded into automatic instruments and equipment for use.

Drawings

FIG. 1 is a perspective view of a food sample pre-processing workstation of the present invention;

FIG. 2 is a front view of the food sample pre-processing station of the present invention;

FIG. 3 is a schematic top-level space diagram of a food sample pretreatment workstation of the present invention;

FIG. 4 is a schematic view of the back structure of the upper space of the food sample pretreatment workstation of the present invention;

FIG. 5 is a schematic view of the lower space of the food sample pre-treatment workstation of the present invention;

FIG. 6 is a schematic view of the back structure of the lower space of the food sample pretreatment workstation of the present invention;

FIG. 7 is a schematic structural diagram of a homogeneous module;

fig. 8 is an exploded view of the homogenous tank.

Wherein: 1-pressurizing motor, 2-pressurizing rod piece, 3-homogenizing tank, 4-homogenizing motor, 5-piston push rod, 6-piston head, 7-tank body, 8-blade, 9-base, 10-motor shaft joint, 11-filtrate port, 12-homogenizing module, 13-big tube storage position, 14-liquid gun storage position A, 15-air pipe A, 16-fan A, 17-big tube incubation position, 18-station switching panel, 19-ultraviolet sterilizing lamp, 20-automatic control module, 21-air pipe B, 22-fan B, 23-homogenizing tank placement opening, 24-pretreatment liquid storage position, 25-magnetic bead storage position, 26-pipetting mechanism A, 27-big tube switch cover mechanism, 28-homogenizing module liquid outlet, 29-grabbing mechanism A30-incubation bottom plate sealing structure, 31-small tube incubation position, 32-sample transfer mechanism A, 33-small tube sample vertical transmission mechanism, 34-large tube sample vertical transmission mechanism, 35-small magnetic rod sleeve storage position, 36-washing liquid storage position, 37-small tube storage position, 38-post-treatment sample storage position, 40-liquid gun storage position B, 41-culture solution storage position, 42-large magnetic rod sleeve storage position, 43-middle tube storage position, 44-middle tube sample working position, 45-large tube sample working position, 46-large magnetic rod separation assembly, 47-liquid transfer mechanism B, 48-middle/small tube switch cover mechanism, 49-small magnetic rod separation assembly, 50-grabbing mechanism B, 51-conveying component, 52-magnetic separation station switching bottom plate, 53-detection water reagent storage position.

Detailed Description

The invention will now be described in detail by way of example with reference to the accompanying drawings.

Example 1:

the embodiment provides a food sample pretreatment workstation, which can realize high-throughput, automatic and rapid treatment of food samples.

As shown in fig. 1 and 2, the food sample pretreatment workstation comprises: the system comprises an automatic control module, an execution module and a biological safety module.

The food sample is preceded by three procedures of homogenization-incubation-separation, based on which the execution module comprises: the device comprises a homogenizing module, an incubation module, a separation module and a transmission module; the whole frame of the workstation is formed by splicing sectional materials; the inside homogeneity station, incubation station and the separation station of being provided with of workstation. In the embodiment, the internal space of the workstation is divided into an upper layer and a lower layer, the homogenizing station and the incubation station are arranged on the upper layer, and the separating station is arranged on the lower layer; the homogenizing module is positioned at the homogenizing station and used for homogenizing the food sample and the culture solution; the incubation module is positioned at an incubation station, the incubation station is provided with an independent openable closed space and is used for incubation treatment in a set temperature environment, and the incubation module comprises a big pipe incubation module and a small pipe incubation module; the separation module is positioned at the separation station and adopts an immunomagnetic separation process to realize rapid enrichment and separation of target microorganisms; the transmission module is used for realizing the transportation of the sample tube among various stations, wherein the sample tube comprises a large centrifuge tube (hereinafter referred to as a large tube), a middle centrifuge tube (hereinafter referred to as a middle tube) and a small centrifuge tube (hereinafter referred to as a small tube); the large tube, the middle tube and the small tube are defined after the volumes of the centrifuge tubes with three different volumes in the scheme are compared, namely, the large tube volume is larger than the middle tube volume and smaller than the small tube volume; as an example, the large tube volume is 50ml, the medium tube volume is 25ml, and the small tube volume is 2ml. The transmission module includes: sample transfer mechanism A32, sample transfer mechanism B, small tube sample vertical transport mechanism 33 and large tube sample vertical transport mechanism 34.

As shown in fig. 3 and 4, the upper space of the workstation is divided into a left part and a right part, the left space is provided with a homogenizing station for installing a homogenizing module, the homogenizing module is fixed on the workstation frame, the back of the homogenizing module is provided with a homogenizing tank placing port 23, and the lower part of the back is provided with a homogenizing module liquid outlet port 28. In the homogenizing module, food samples in the homogenizing tank are fully contacted with the culture solution in a shredding, stirring and uniformly mixing mode, so that the homogenizing effect is good, and the recovery rate of pathogenic bacteria is high; and the homogenizing mode has small impact vibration. The homogenization module has a plurality of homogenization tank assemblies therein, each homogenization tank assembly comprising a plurality of homogenization tanks (in this example, each homogenization tank assembly comprising 4 homogenization tanks); in this example, the volume of a single homogenization tank was 125ml, and the homogenization tank was filled with a food sample to be homogenized and a set amount (65 ml) of culture solution; the homogenizing tank is internally provided with a blade, the cutting and stirring are realized by driving the blade to rotate, and when the food sample to be homogenized is liquid, the food sample to be homogenized and the culture solution are stirred; when the food sample to be homogenized is solid, the food sample and the culture solution are stirred while the food sample to be homogenized is minced. The two layers of filter screens are arranged on the base of the homogenizing tank, the bottom is provided with a filtrate port, and after the homogenizing process is finished, the culture solution in the homogenizing tank can be discharged from the filtrate port at the bottom by pressurizing the homogenizing tank. In addition, the homogenizing tank work position is also provided with a heating unit for in-situ heating of food samples and culture fluid in the homogenizing tank, so that the food samples and the culture fluid can be pre-incubated while being uniformly mixed.

The right side of the upper space is divided into a front part, a middle part and a rear part; the front side is provided with a station switching panel 18, and a large pipe storage position 13, a combined storage position and a large pipe incubation position 17 (the large pipe incubation position 17 is provided with a large pipe incubation module) which are sequentially arranged on the station switching panel 18 from left to right; wherein the combined storage position is provided with a liquid gun storage position A14, a magnetic bead storage position 25 and a pretreatment liquid storage position 241; a plurality of groups of large centrifuge tubes (one group for each row) are stored on the large tube storage position 13, each group comprises the same number of large centrifuge tubes as the number of the homogenizing cans in each homogenizing can assembly, and in the example, 4 large centrifuge tubes are arranged as one group for each row and correspond to the homogenizing cans in each homogenizing can assembly one by one; correspondingly, for convenience of operation, the liquid gun storage position A14, the magnetic bead storage position 25, the pretreatment liquid storage position 24 and the large tube incubation position 17 are also provided with 4 stations in a row as a group. The station switching panel 18 can be moved back and forth along a guide rail provided on the frame under the control of the automatic control module to perform position switching. A vertical mounting plate is arranged in the middle of the right side of the upper space and fixed on the frame, and an air pipe A15 and a fan A16 (part of the biological safety module) are arranged above the front side surface of the vertical mounting plate; a large pipe switch cover mechanism 27, a pipetting mechanism A26 and a grabbing mechanism A29 are sequentially arranged on the rear side surface of the vertical mounting plate from left to right. The position of the large tube switch cover mechanism 27 corresponds to the position of the large tube storage position 13, the position of the pipetting mechanism A26 corresponds to the position of the combined storage position, and the grabbing mechanism A29 corresponds to the position of the large tube incubation position 17. In addition, the right rear part of the upper space is also provided with a small pipe incubation position 31 (used for placing a small pipe incubation module) and a sample transfer mechanism A32, and the sample transfer mechanism A32 is arranged on the inner side, close to the rear of the cabinet, of the large pipe switch cover mechanism 27, the pipetting mechanism A26 and the grabbing mechanism A29 and used for transferring large pipes.

When the station switching panel 18 moves backwards for a set distance, a group of large pipes in the large pipe storage position 13 can be located just below the large pipe switch cover mechanism 27, the combined storage position is located just below the pipetting mechanism A26, and the large pipe incubation position 17 is located just below the grabbing mechanism A29. The large pipe switch cover mechanism 27 clamps the large pipes in the group, four cover opening units are correspondingly arranged on the large pipe switch cover mechanism 27, the large pipe switch cover mechanism 27 is used for opening the cover of the large pipe and placing the large pipe after opening the cover on the sample transfer mechanism A32 (the large pipe switch cover mechanism 27 clamps the cover after opening the cover and is used for subsequent light covers); the sample transferring mechanism A32 transfers the uncapped large pipe to the combined storage position, the pipetting mechanism A picks up the gun head (1 ml gun head) in the liquid gun storage position A, then a preset amount of pretreatment liquid is sucked in the pretreatment liquid storage position 24, and the sucked pretreatment liquid is added into the uncapped large pipe; then, the sample transfer mechanism a32 transfers the large pipe added with the pretreatment liquid to the position of the liquid discharge port 28 of the homogenizing module in the homogenizing station, controls the liquid discharge of the homogenizing module (discharges the homogenized liquid), and injects the homogenized liquid into the large pipe; then the sample transferring mechanism A32 transfers the large tube to the combined storage position, the pipetting mechanism A picks up the gun head (1 ml gun head) in the liquid gun storage position A again, and then pumps the immunomagnetic bead reagent in the magnetic bead storage position, and adds the sucked immunomagnetic bead reagent with a set amount into the large tube; finally, the large pipe is transported to the lower part of the large pipe switch cover mechanism 27 by the sample transport mechanism A32, and after the large pipe switch cover mechanism 27 is closed to seal the large pipe, the large pipe is further transported to an incubation module on the large pipe incubation position 17 by the sample transport mechanism A32 for incubation. The whole incubation module can rotate around a rotation shaft which is horizontally arranged, and is evenly mixed and incubated for a set time at a set temperature. The large pipe incubation position 17 and the small pipe incubation position are sealed and isolated by the incubation bottom plate sealing structure 30, so that heat dissipation is reduced, and the temperature of an incubation environment is ensured; the bottom plate seal 30 can be opened to allow for sample transfer channels when sample tube transfer is desired.

As shown in fig. 5 and 6, a separation module is arranged in the lower space of the inner space of the workstation, and the immunomagnetic separation process is adopted to realize the rapid enrichment and separation of target microorganisms. The whole lower space is divided into a front part, a middle part and a rear part; the front side is provided with a magnetic separation station switching bottom plate 52, a small pipe storage position 37, a small magnetic rod sleeve storage position 35, a treated sample storage position 38, a washing liquid storage position 36, a water detection reagent storage position 53, a culture solution storage position 41, a large magnetic rod sleeve storage position 42 and a middle pipe storage position 43 which are sequentially arranged on the magnetic separation station switching bottom plate 52 from left to right; the front sides of the washing liquid storage position 36, the detection water reagent storage position 53 and the culture solution storage position 41 are respectively provided with a liquid gun storage position B40 which is used for respectively storing gun heads for sucking washing liquid, detection water reagent and culture solution; it is understood that the components of the lower space (except the transport component) all belong to separate modules.

The lower floor space middle part is provided with vertical mounting panel, and vertical mounting panel is fixed on the frame, and the top of the leading flank of vertical mounting panel is provided with tuber pipe B21 and tuber pipe B22, in addition, still is equipped with ultraviolet sterilizing lamp 19 on this mounting panel (biological safety module's part, not schematic diagram in fig. 5, see fig. 2). A middle/small tube switch cover mechanism 48, a small magnetic rod separation assembly 49, two groups of pipetting mechanisms B47 (the two groups of pipetting mechanisms B47 respectively correspond to front liquid guns for picking up and sucking washing liquid and culture liquid), a large magnetic rod separation assembly 46 and a grabbing mechanism B50 are sequentially arranged on the rear side surface of the mounting plate from left to right; in addition, a small pipe sample vertical transmission mechanism 33, a large pipe sample vertical transmission mechanism 34 and a sample transfer mechanism B are arranged at the rear part of the lower layer space (not shown in the figure); the small pipe sample vertical transmission mechanism 33 and the large pipe sample vertical transmission mechanism 34 are provided with a carrying component 51 serving as an execution end; the upper end of the large tube sample vertical transmission mechanism 34 corresponds to the position of the large tube incubation position 17 in the upper space, and the lower end corresponds to the position of the sample transfer mechanism B; the upper end of the small tube sample vertical transmission mechanism 33 corresponds to the position of the small tube incubation position 31 in the upper space, and the lower end corresponds to the position of the sample transfer mechanism B (when transmission is needed, the incubation bottom plate sealing structure is opened to give up a transmission channel). The sample transferring mechanism B is provided with a middle tube sample working position 44 and a large tube sample working position 45 (a group of four is arranged in a row) which are used for placing the middle tube and the large tube in a working state, and the middle tube sample working position 44 and the large tube sample working position 45 are arranged in parallel front and back.

After homogenization and incubation are completed in the upper space, namely after incubation time of the large tube in an incubation module on the large tube incubation position 17 reaches a set time, the large tube is firstly transported to the lower part of the large tube switch cover mechanism 27 by the sample transport mechanism A32, the large tube switch cover mechanism 27 performs cover opening action (cover is discarded at the moment), the large tube after cover opening is further transported to the large tube incubation position 17 by the sample transport mechanism A32, the large tube after cover opening is grabbed on the large tube incubation position 17 by the grabbing mechanism A29, the large tube is vertically placed on the large tube sample vertical transport mechanism 34, and is transported to the large tube sample working position 45 of the lower space through the large tube sample vertical transport mechanism 34 so as to carry out subsequent separation work.

The separation module performs two magnetic separation processes according to the set process, namely, the first magnetic separation: before the large tube sample vertical transmission mechanism 34 is transmitted, the magnetic separation station switching bottom plate 52 moves backwards for a set distance, and the middle tube sample working position 44 and the large tube sample working position 45 are moved to the position right below the grabbing mechanism B50 through the sample transfer mechanism B; the carrying part 51 on the large pipe sample vertical transmission mechanism 34 carries the large pipe on the sample vertical transmission mechanism 34 to the large pipe sample working position 45; simultaneously, the grabbing mechanism B50 grabs the middle tube on the middle tube storage position 43 and places the middle tube on the middle tube sample working position 44; transferring the large tube to the position below the large magnetic rod separation assembly 46 through the sample transferring mechanism B; the large magnetic rod in the large magnetic rod separating assembly 46 completes the sleeving of the large magnetic rod sleeve on the large magnetic rod sleeve storage position 42; then inserting the large magnetic rod sleeved with the large magnetic rod sleeve into the large pipe, and standing and separating for a set time (the large pipe is limited on the large magnetic rod separating assembly 46 at the moment); after the set time of standing separation, the large magnetic rod separating assembly 46 separates the large pipe (i.e., the large pipe is discarded, and a collecting frame is provided below the large magnetic rod separating assembly 46). In the process of standing and separating the large tube, the sample transferring mechanism B moves to enable the middle tube sample working position 44 to move below the middle/small tube switch cover mechanism 48, and after the cover opening of the middle tube is finished; the sample transfer mechanism B moves to move the middle tube sample working position 44 to the lower part of the pipetting mechanism B47, the pipetting mechanism B47 picks up the gun head in the liquid gun storage position B40 in front of the culture liquid storage position 41, a set amount (1 mL) of culture liquid is sucked in the culture liquid storage position 41 through the gun head, and the culture liquid is added into the middle tube, and the culture liquid can be repeated once according to the requirement, so that 2mL of culture liquid is added into the middle tube altogether. Then the sample transferring mechanism B moves to enable the middle tube sample working position 44 to move the middle tube filled with the culture solution to the lower part of the large magnetic rod separating component 46, and the large magnetic rod and the magnetic rod sleeve are inserted into the sterile middle tube filled with the culture solution; then the large magnetic rod separating component 46 controls the large magnetic rod to retract, so that the magnetic rod is sleeved in the middle pipe; and the large magnetic rod separation assembly 46 is utilized to move up and down to enable the magnetic rod to be sleeved in the sterile middle tube to vibrate up and down for a plurality of times, so that the immune magnetic bead compound is resuspended in the culture solution, and an immune magnetic bead compound solution is formed; then, the large magnetic rod separating assembly 46 drives the magnetic rod sleeve to ascend and move out of the middle pipe, and then the magnetic rod sleeve is discarded (the magnetic separation process can be repeated once).

Thereafter, the middle/small tube opening and closing cover mechanism grabs the small tube on the small tube storage position 37, and places the small tube on the sample transfer mechanism B after uncovering; the sample transfer mechanism B moves to enable the middle tube sample working position 44 and the small tube after the switch to move to the lower part of the liquid transfer mechanism B47, the liquid transfer mechanism B47 picks up the gun head in the liquid gun storage position B40, and a set amount (1 mL) of immunomagnetic bead compound solution is sucked in the middle tube through the gun head and is added into the small tube.

When secondary incubation is required, the sample transfer mechanism B moves to enable the small tube to move to the position below the middle/small tube cover opening and closing mechanism 48 for cover closing operation; after the cover is closed, the sample transferring mechanism B moves to enable the small tube to move to the position of the small tube sample vertical transferring mechanism 33, the small tube is transferred onto the small tube sample vertical transferring mechanism 33 by a transferring component on the small tube sample vertical transferring mechanism 33, and the small tube is transferred to the small tube incubation position 31 of the upper space by the small tube sample vertical transferring mechanism 33 for secondary incubation (rotating culture for 1-5 h at a proper temperature). And after the secondary incubation is finished, conveying the small tube to a sample transfer mechanism B in the lower space again for secondary separation.

When secondary incubation is not required, secondary magnetic separation is performed directly.

The process of the secondary magnetic separation is the same as the process of the primary separation described above. Firstly, transferring the small tube to the lower part of the middle/small tube switch cover mechanism through the sample transferring mechanism B, and completing the cover opening operation; the small magnetic rod of the small magnetic rod separating assembly 49 completes the sleeving of the small magnetic rod sleeve on the small magnetic rod sleeve storage position 35; then the small tube is transported to the lower part of the small magnetic rod separating assembly 49 through the sample transporting mechanism B, the small magnetic rod sleeved with the small magnetic rod sleeve is inserted into the small tube, the small tube is kept stand for separating for a set time (at the moment, the small tube is limited on the small magnetic rod separating assembly 49), and the small tube is discarded after the small tube is kept stand for separating. In the process of standing and separating the tubules, the medium/tubule switch cover mechanism grabs another group of tubules (new tubules) on the tubule storage position 37 again, and the tubules are placed on the sample transfer mechanism B after being opened; the sample transferring mechanism B moves to enable the new small tube to move below the pipetting mechanism B47, the pipetting mechanism B47 picks up a gun head in the liquid gun storage position B40 at the front side of the washing liquid storage position 36, a set amount (0.5 mL) of culture washing liquid is sucked in the washing liquid storage position 36 through the gun head, and the culture washing liquid is added into the new small tube; then the sample transferring mechanism B moves to enable the new small pipe filled with the washing liquid to move below the small magnetic rod separating assembly 49, and the small magnetic rod and the magnetic rod sleeve are inserted into the new small pipe filled with the washing liquid; then the small magnetic rod separating component 49 controls the small magnetic rod to retract, so that the magnetic rod is sleeved in a new small pipe; and the small magnetic rod separating assembly 49 is utilized to move up and down to make the magnetic rod sleeve vibrate up and down in the new small pipe for several times to form immune magnetic bead compound solution; the small magnetic rod separating assembly 49 then drives the magnetic rod sleeve up and out of the new small tube, and the magnetic rod sleeve is discarded. Repeating the steps twice, then moving the new small tube to the lower part of the liquid transferring mechanism B47 by the sample transferring mechanism B, picking up the gun head in the liquid gun storage position B40 at the front side of the detection water reagent storage position 53 by the liquid transferring mechanism B47, sucking the detection water reagent with a set quantity (50 uL) in the detection water reagent storage position 53 through the gun head, and adding the detection water reagent into the new small tube; finally, the sample transfer mechanism B moves the new vial to the position below the middle/small tube opening and closing cover mechanism 48 to close the cover and place the new vial in the processed sample storage position 38.

The automatic control module 20 (such as a computer) is arranged on the workstation frame, and working conditions and modes are preset in the automatic control module 20, so that the execution module automatically performs the pretreatment work according to the preset working conditions and modes, and can preset working conditions with different bacteria characteristics and preset food characteristic modes. The different bacteria characteristics are classified into salmonella, paracolytics, shigella and staphylococcus aureus. The predetermined food characteristics are classified into brittle solids such as vegetables and fruits, nonfriable solids such as meats, and liquids such as milk and fruit juices.

The biological safety module comprises an air pipe A15, a fan A16, an air pipe B21, a fan B22, an ultraviolet sterilizing lamp arranged above the inside of the homogenizing module and an ultraviolet sterilizing lamp 19 arranged at the side edge of the discarding station; is an important component for the pretreatment or detection of microorganisms, and aims to eliminate the cross contamination between samples in the same batch in the running process of the instrument. According to the preset working conditions and modes, the trend of air flow is reasonably distributed between the sample test area and the operation area, and in particular, the sample position of each operation area is perpendicular to the air flow direction, so that even if the sample tube is uncapped or the reagent is transferred, aerosol operation is easy to occur, and cross contamination of each sample can not occur. And the bottom edge of each operation area generates transverse air flow which sequentially passes through the operation area and the waste liquid area, and HEPA filters are arranged beside the waste liquid area to filter microorganisms in the air flow and discharge clean air. An ultraviolet sterilizing lamp 19 is located in the region where the sample tube contacts.

After the processing is finished, the automatic control module 20 sends a signal to the biosafety module, starts a biosafety processing program, starts an ultraviolet sterilizing lamp 19 positioned in a region contacted with the sample tube, eliminates cross contamination between samples in the same batch in the running process of the instrument, and closes the workstation after the ultraviolet processing is finished.

Example 2:

on the basis of the above embodiment 1, a specific structural schematic diagram of the homogenizing module is given in this embodiment;

as shown in fig. 7, the homogenizing module includes: a housing 15, a homogenization tank support bracket 16, a homogenization tank assembly, a pressurizing unit, a piston push rod 5 and a homogenization motor 4.

The homogenizing tank support frame 16 is transversely supported inside the casing 15 through a support shaft, can rotate along the axial direction of the support shaft and is used for uniform mixing after homogenization, so that the support shaft is a homogenizing module revolution shaft, and the homogenizing module revolution shaft can rotate around the axis of the homogenizing module revolution shaft under the drive of the revolution driving mechanism so as to drive the homogenizing tank support frame 16 to rotate; as an example, the revolution driving mechanism includes a revolution driving motor and a belt transmission mechanism for connecting a power output shaft of the revolution driving motor and a revolution shaft of the homogenizing module; the revolution driving motor and the revolution shaft of the homogenizing module can be arranged in parallel through the belt transmission mechanism, so that the size of the homogenizing module is reduced.

A plurality of homogenizing tank assemblies are arranged on the homogenizing tank support frame 16, and the homogenizing tank assemblies are supported on the homogenizing tank support frame 16 through a transversely arranged homogenizing module rotation shaft; a plurality of homogenizing tank components are uniformly distributed on the homogenizing tank support frame 16 at intervals along the circumferential direction; each homogenization tank assembly is supported on a homogenization tank support bracket 16 by a homogenization module spin shaft. The homogenization tank support bracket 16 is capable of rotating (i.e., spinning) about the axis of its own homogenization module spin shaft.

The homogenizing tank assembly comprises a mounting frame and a plurality of homogenizing tanks 3 mounted on the mounting frame; in the embodiment, four homogenizing tank placing grooves are transversely arranged on each mounting frame, and one homogenizing tank 3 is limited in each homogenizing tank placing groove; thus, at most 16 homogenizing tanks 3 can be placed in one homogenizing module, and each homogenizing tank 3 is filled with 25g of sample and 25-65 mL of culture solution; and each homogenizing tank 3 can be filled with different samples and culture solutions, and the samples and the culture solutions are independent from each other.

Each homogenizing tank assembly can be driven by a rotation driving mechanism respectively; or driven by a rotation driving mechanism to synchronously rotate; when the rotation driving mechanism is adopted, all the rotation shafts of the homogenizing modules are connected through the belt transmission mechanism, one of the rotation shafts of the homogenizing modules is a driving shaft and is connected with the rotation driving motor, and the other rotation shafts of the homogenizing modules are driven shafts, so that the rotation shafts of all the homogenizing modules are driven to synchronously rotate through the rotation driving motor and the belt transmission mechanism, and the rotation of each homogenizing tank assembly is realized.

As shown in fig. 8, the homogenizing tank includes: the cylinder 7, the piston head 6, the base 9 and the blade 8; the top opening of the tank body 7 is coaxially provided with the piston head 6, the piston head 6 can downwards squeeze the culture solution in the tank body 7 under the pushing of the pressurizing unit supported on the shell 15 (after one homogenization is finished, the homogenizing tank is detached for cleaning, so that in the automatic homogenization process, the piston head 6 is only pushed to move downwards, and the piston head 6 does not return when working); the outer circumferential surface of the piston head 6 is provided with a sealing ring, so that the sealing with the inner circumferential surface of the tank body 7 is realized. The bottom opening of the tank body 7 is closed by a base 9, and a sealing ring is arranged on the outer circumferential surface of the base 9 to realize sealing; the upper end surface of the base 9 is connected with a blade 8 through a rotating shaft which is coaxially arranged with the tank 7; the blade 8 is capable of stirring while chopping the food sample as it rotates. As an example, the blade 8 has a certain inclination angle so that stirring can be performed while cutting (the rotation of the blade 8 has an upward lifting effect on the culture liquid, so that the culture liquid forms a vortex in the vertical direction, and the homogenizing effect is improved). The shaft extending base 9 connected with the blade 8 is connected with the motor shaft joint 10, the motor shaft joint 10 is detachably connected with the homogenizing motor 4 supported on the housing 15 (i.e. the motor shaft joint 10 and the homogenizing motor are not fixedly connected together, and the homogenizing motor is connected with the motor shaft joint 10 only when homogenization is needed, and disconnected after homogenization is finished), and the homogenizing motor 4 drives the blade 8 to rotate after the motor shaft joint 10 is connected with the homogenizing motor 4.

The upper end surface of the base 9 is provided with a filter screen, so as to remove massive residues after the food sample is homogenized, and filter out clear liquid; the lower end face is provided with a filtrate port 11 having a one-way valve, and the filtered clear liquid flows out from the filtrate port 11.

Further, the homogenizing tank is also provided with a heating unit for heating the sample inside the homogenizing tank to a set temperature. As an example, an electric heating device is provided on the base 9, such as an electric heating wire provided inside the base 9.

A pressurizing unit is arranged above the homogenizing tank support frame 16 in the shell 15 (the pressurizing unit is arranged on the shell 15 and does not rotate along with the homogenizing tank support frame 16); the pressurizing units are in one-to-one correspondence with the plurality of homogenizing tanks in the single homogenizing tank assembly; when the homogenizing tank support 16 is rotated to a position where one of the homogenizing tank assemblies is located below the pressurizing unit, the homogenizing tank assembly is in the working position; at this time, the pressurizing unit can be extended and contacted with the piston head 6 of the homogenizing tank corresponding to the pressurizing unit, and the corresponding piston head 6 is pushed to press the culture medium in the tank 7 downwards.

As an example, the pressurizing unit includes a pressurizing motor 1 supported on a housing 15 and a piston rod 5 connected to a power output end of the pressurizing motor 1 through a pressurizing rod 2; the pressurizing motor 1 is a linear motor, and the power output end of the pressurizing motor performs linear motion; the power output end of the pressurizing motor 1 is vertically arranged, and is coaxially connected with the piston push rod 5 through the pressurizing rod piece 2, so that the piston push rod 5 can be driven in the vertical direction, and the corresponding piston head 6 is pushed to downwards extrude the culture solution in the tank body 7.

In this example, whereas four homogenization tanks 3 are arranged in the lateral direction in each homogenization tank assembly, four pressurizing units are fixed to the housing 15 correspondingly; the four pressurizing units are in one-to-one correspondence with the four homogenizing tanks 3 in the vertical state.

Inside the housing 15, a homogenizing motor 4 is arranged below the homogenizing tank support frame 16 (the homogenizing motor 4 is mounted on the housing 15 through a motor base and does not rotate along with the homogenizing tank support frame 16); and the homogenizing motor 4 corresponds to a plurality of homogenizing tanks in a single homogenizing tank assembly one by one; when the homogenizing tank support 16 rotates to a position where one of the homogenizing tank assemblies is above the position of the homogenizing motor 4, the homogenizing tank assembly is in the working position; the motor shaft of the homogenizing motor 4 is inserted into a motor shaft joint 10 at the bottom of the homogenizing tank 3 at the corresponding position and is used for driving a blade 8 in the tank body 7 to rotate. As an example, the detachable connection between the motor shaft of the homogenizing motor 4 and the motor shaft joint 10 is as follows: when the homogenizing tank support frame 16 rotates to a position above the position of the homogenizing motor 4, the motor base is controlled to move upwards so as to drive the homogenizing motor 4 to move upwards, so that a motor shaft of the homogenizing motor 4 is inserted into a motor shaft joint 10 (such as a connecting hole with a regular hexagon is formed in the center of the motor shaft joint 10) at the bottom of the homogenizing tank 3 at the corresponding position, a connector matched with the motor shaft end of the homogenizing motor 4 is formed at the motor shaft end of the homogenizing motor 4, and the rotating shaft connected with the blade 8 can be driven to rotate by the homogenizing motor 4 after the connector is inserted into the connecting hole); after homogenization is finished, the homogenization motor is stopped, the motor base is controlled to move downwards, so that the motor shaft of the homogenization motor 4 is withdrawn from the motor shaft joint 10, and the homogenization motor and the motor base are separated.

In this example, in view of four homogenizing tanks 3 arranged in the transverse direction on each homogenizing tank assembly, four homogenizing motors 4 are correspondingly arranged on the housing 15, and the four homogenizing motors 4 are in one-to-one correspondence with the four homogenizing tanks 3 in the vertical state; and a group of four homogenizing motors 4 share a motor base (or the four motor bases are fixedly connected into a whole), so that synchronous lifting is realized.

The working principle of the homogenizing module is as follows:

firstly, controlling the homogenizing tank support frame 16 to rotate around the axis of the public rotation shaft of the homogenizing module so as to enable one of the homogenizing tank components to be in a vertical state (the homogenizing tank is filled with food samples and culture solution to be homogenized); then the motor base is controlled to move upwards, so that the motor shaft of the homogenizing motor 4 is inserted into a motor shaft joint 10 at the bottom of the homogenizing tank 3 at the corresponding position; then, starting the homogenizing motor 4, rotating the rotating blade 8 around the axis of the tank body 7 through the rotating shaft, and stirring the sample in the homogenizing tank through the rotating of the rotating blade 8 around the axis of the tank body 7 when the food sample is a liquid food sample; when the food sample is a solid food sample, the rotating shaft rotating blade 8 rotates around the axis of the tank body 7 to cut and stir the sample in the homogenizing tank, so that the aim of homogenizing is fulfilled, and the effect is that the contact area with the culture solution is increased; after homogenization is finished, the homogenization motor is stopped, and a motor shaft joint 10 at the bottom of the homogenization tank 3 is separated from the homogenization motor 4; thereby sequentially homogenizing the sample in the homogenization tank 3 in each homogenization tank assembly.

Then at a proper temperature (36 ℃), controlling the rotation of the homogenizing tank support frame 16 to realize revolution and controlling the rotation of each homogenizing tank assembly; whereby mixing is achieved whereby the sample in the homogenization tank 3 is mixed and incubated for a period of time in sufficient contact with the culture broth.

After the mixing incubation is finished, each homogenizing tank assembly is rotated to a vertical state in sequence, the pressurizing motor 1 is started, the piston push rod 5 extends out, the piston push rod 5 pushes the piston head 6 to downwards squeeze the culture solution in the tank body 7, and the culture solution in the homogenizing tank 3 is discharged through the filtrate port 11 at the bottom end inside the homogenizing tank.

In the homogenizing module, the automation of the homogenizing process can be realized by controlling each driving component (comprising a homogenizing motor, a pressurizing motor, a revolution driving motor, a rotation driving motor and the like); and homogenizing the food sample according to a preset program.

Example 3:

on the basis of the above embodiment 2, further, the inner wall surface of the tank 7 has a wear-resistant coating of 200 nm-500 nm, so that the wear resistance of the tank 7 is increased, the friction between the piston head 6 and the inner wall surface of the tank 7 is increased, and the tightness of the homogenizing tank in the process of uniformly mixing is improved.

Further, the thickness of the wear-resistant coating is gradually decreased from top to bottom (the thickness of the wear-resistant coating is nm, and sealing between the piston head 6 and the tank body 7 can still be ensured), but the resistance is small when the piston head 6 moves downwards, otherwise the resistance is large (namely, the resistance to downward movement is smaller than the resistance to upward movement), so that the possibility of liquid leakage caused when the homogenizing tank rotates and uniformly incubates after homogenizing (namely, the liquid culture in the tank body pushes the piston head 6 to move upwards when the homogenizing tank rotates and uniformly incubates is avoided), the reliability of the homogenizing tank is improved, and biological cross contamination is effectively prevented.

In summary, the above embodiments are only preferred embodiments of the present invention, and are not intended to limit the scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A food sample pretreatment workstation, characterized in that: a homogenizing station, an incubation station and a separation station are arranged in the workstation;

2. The food sample pre-processing workstation of claim 1, wherein: the internal space of the workstation is divided into an upper layer and a lower layer, the homogenizing station and the incubation station are arranged on the upper layer, and the separating station is arranged on the lower layer;

3. The food sample pre-processing workstation of claim 2, wherein: the rear part of the right side of the upper space is also provided with a small pipe incubation position for placing a small pipe incubation module; the transmission module further comprises: and the small pipe sample vertical transmission mechanism is connected with the upper layer and the lower layer.

4. A food sample pre-processing workstation as claimed in claim 3, wherein: the large pipe incubation position and the small pipe incubation position are sealed and isolated by an openable incubation bottom plate sealing structure.

5. The food sample pre-processing workstation of claim 3 or 4, wherein: the whole lower space of the workstation is divided into a front part, a middle part and a rear part;

6. The food sample pre-processing workstation of any one of claims 1-4, wherein: also comprises a biological safety module;

7. The food sample pre-processing workstation of any one of claims 1-4, wherein: the automatic control module is used for controlling the homogenizing module, the incubation module and the separation module to automatically operate according to preset working conditions and modes.

8. The food sample pre-processing workstation of any one of claims 1-4, wherein: the homogenizing module comprises: the device comprises a shell, a homogenizing tank support frame, a homogenizing tank assembly, a pressurizing assembly and a homogenizing driving assembly;

9. The food sample pre-processing workstation of claim 8, wherein: the homogenizing tank assembly is supported on the homogenizing tank support frame through a homogenizing module rotating shaft; the homogenizing module rotating shaft can drive the homogenizing tank assembly to rotate around the axis of the homogenizing tank assembly to realize autorotation.

10. A food sample pre-processing workstation as claimed in claim 8 or 9, wherein: the homogenizing tank includes: the device comprises a tank body, a piston head, a base and a blade;