CN115885032A

CN115885032A - Integrated intelligent nucleic acid detection system

Info

Publication number: CN115885032A
Application number: CN202080103157.0A
Authority: CN
Inventors: 林思远; 李景; 樊清华; 邹良英; 彭友雄; 余德健; 蒋慧
Original assignee: MGI Tech Co Ltd
Current assignee: MGI Tech Co Ltd
Priority date: 2020-08-28
Filing date: 2020-08-28
Publication date: 2023-03-31
Also published as: CN115885032A8; WO2022041139A1; ZA202303702B

Abstract

The integrated intelligent nucleic acid detection system comprises a sample processing unit, a sample analysis unit and a control unit, wherein the sample processing unit is used for extracting a nucleic acid sample in a sample to be detected; the sample detection unit is used for detecting the nucleic acid sample to obtain a detection result; and the information management unit is used for acquiring and outputting the detection result. The application provides an intelligent nucleic acid detecting system of integrated form can realize quick automatic nucleic acid detection.

Description

Integrated intelligent nucleic acid detection system

Technical Field

The present application relates to an integrated intelligent nucleic acid detection system.

Background

With the development of new coronavirus (2019-nCoV) epidemic situation, the virus detection and identification speed, flux and safety are required to be faster, more flexible and safer. Most of the existing detection means are carried out in a fixed laboratory, so that the defects of long detection time, low detection efficiency, low automation degree and the like exist, and potential safety hazards exist for operators.

Content of application

In view of the above, there is a need for an integrated intelligent nucleic acid detecting system capable of automatic detection with high efficiency.

The application provides an integrated form intelligence nucleic acid detecting system, includes:

and the sample processing unit is used for extracting the nucleic acid sample in the sample to be detected.

And the sample detection unit is used for detecting the nucleic acid sample to obtain a detection result.

And the information management unit is used for acquiring and outputting the detection result.

In this embodiment, the sample processing unit includes at least one sample processing device, and the sample processing device is used for extracting the nucleic acid sample from the sample to be detected.

In this embodiment, the sample processing device is a nucleic acid extractor.

In this application embodiment, the sample processing unit further includes at least one sample dispensing device, and the sample dispensing device is used for dispensing the sample to be detected.

In this embodiment, the sample detection unit includes at least one detection device, and the detection device is an RT-PCR detector.

In this application embodiment, integrated form intelligence nucleic acid detecting system includes an at least laboratory, each the laboratory includes a box and set up in sample process chamber and sample detection room in the box, sample process unit set up in the sample process chamber, sample detection unit set up in the sample detection room.

In this application embodiment, the laboratory further includes a sample transfer device and a sample detection transfer device, the sample processing chamber receives a sample to be detected through the sample transfer device, and the sample detection chamber is communicated with the sample processing chamber through the sample detection transfer device.

In this embodiment of the present application, the laboratory further includes a reagent preparation chamber, and a reagent preparation unit is disposed in the reagent preparation chamber and is used for providing a detection reagent.

In this embodiment, the laboratory further comprises a reagent transfer device, and the sample processing chamber is communicated with the reagent preparation chamber through the reagent transfer device.

In this application embodiment, the integrated intelligent nucleic acid detecting system includes two sets of laboratories, and the two sets of laboratories are communicated with each other through another sample transfer device.

Compared with the prior art, the integrated intelligent nucleic acid detection system provided by the application can realize rapid and automatic nucleic acid detection, is high in detection efficiency and flexible in flux, and meets the requirements of safety prevention and control.

Drawings

FIG. 1 is a block diagram of an integrated intelligent nucleic acid detection system according to an embodiment of the present disclosure.

FIG. 2 is a flow chart of information management for an integrated intelligent nucleic acid detection system according to an embodiment of the present disclosure.

FIG. 3 is a schematic structural diagram of an integrated intelligent nucleic acid detecting system provided in a second embodiment of the present application.

FIG. 4 is a schematic structural diagram of an integrated intelligent nucleic acid detecting system provided in a third embodiment of the present application.

FIG. 5 is a schematic diagram of an integrated intelligent nucleic acid detecting system according to a fourth embodiment of the present application.

Description of the main elements

Integrated intelligent nucleic

acid detection systems

100, 200, 300, 400

Environment control unit 11

Reagent preparing Unit 12

Reagent preparation chamber 23

Storage device 121

Clean bench 122

Sample transfer device 13

Reagent delivery device 14

Sample processing unit 15

Sample processing chamber 25

Sample processing device 151

Sample dispensing apparatus 152

Sample transfer device 16

Sample detection unit 17

Sample detection chamber 27

Detection device 171

Information management unit 18

Air conditioner and electromechanical chamber 22

Ventilation device 111

Water supply and drainage device 112

Power supply device 113

Central management station 114

Case 21

Sealed door 211

First dressing room 24

The first decontamination basin 241

Buffer room 26

Second dressing room 28

High pressure sterilizing device 281

Second decontamination basin 282

The following detailed description will further illustrate the present application in conjunction with the above-described figures.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without any creative effort belong to the protection scope of the present application.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

The system embodiments described below are merely illustrative, and the division of the modules or circuits is merely a logical division, and other divisions may be realized in practice. Furthermore, it will be obvious that the term "comprising" does not exclude other elements or steps, and the singular does not exclude the plural. Several units or mechanisms recited in the system claims may also be implemented by one and the same unit or mechanism in software or hardware. The terms first, second, etc. are used to denote names, but not any particular order.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1, an integrated intelligent nucleic acid detecting system 100 for detecting a nucleic acid sample, particularly for detecting a new coronavirus nucleic acid sample, includes a laboratory, and the laboratory includes a reagent preparing unit 12, a sample transferring device 13, a reagent transferring device 14, a sample processing unit 15, a sample detecting transferring device 16, a sample detecting unit 17, an information management unit 18, and an environment control unit 11.

The reagent preparation unit 12 is used to store necessary detection reagents. The reagent preparation unit 12 specifically includes a storage device 121 and a clean bench 122, the storage device 121 is used for storing the detection-related reagent, and the clean bench 122 is used for performing a reagent preparation operation.

In the present embodiment, the reagent preparation unit 12 may further include necessary devices such as a pipette set, a small centrifuge, and a vortex mixer.

The sample processing unit 15 is used for extracting a sample to be detected to obtain a nucleic acid sample, and specifically includes a sample processing device 151.

In this embodiment, the detection reagent is transferred from the reagent preparation unit 12 to the sample processing unit 15 by the reagent transfer device 14. The sample to be tested is received by the sample transfer device 13.

In this embodiment, the sample processing device 151 is used to extract a nucleic acid sample from a sample to be detected. The sample processing device 151 is specifically a nucleic acid extraction device (e.g., an RT-PCR instrument) and is mainly used for extraction of RNA. The method can specifically adopt but not limited to a high-throughput automatic sample preparation system (model: MGISP-960, 192 cases/8 h-576 cases/24 h of theoretical flux, or MGISP-100B, 192 cases/8 h-576 cases/24 h of theoretical flux) developed by Shenzhen Huazhi Dazhi science and technology Limited. Can realize high flux nucleic acid extraction, greatly improves the detectionEfficiency; the method can automatically complete the steps from the extraction of the nucleic acid sample to the configuration of an RT-PCR reaction system, and only needs a small amount of conventional auxiliary equipment; integrates multiple functions, occupies a floor area of less than 0.5m ³ (ii) a The suction head with the filter element is used, so that cross contamination is prevented, the pipetting range is as low as 2 mu L, and better extraction efficiency can be ensured by good pipetting precision. The kit is compatible with an RT-PCR system and a third magnetic bead method RNA extraction kit (MGIEasy nucleic acid extraction kit), has good extraction stability and high nucleic acid recovery rate, and can ensure the extraction success rate of low-concentration samples.

In another embodiment, the sample processing unit 15 further includes at least one sample dispensing device 152, the sample dispensing device 152 is mainly used for automatically dispensing the sample to be detected, and the dispensed sample to be detected enters the sample processing device 151 for nucleic acid extraction. The method can be particularly used for automatically extracting the high-flux samples by adopting a full-automatic cup separation processing system (MGISTP-7000, 192 cases/40 min) developed by Shenzhen Hua Dazhi science and technology industries, ltd. The full-automatic cup separation processing system can be adapted to the high-throughput automatic sample preparation system. The sample split charging device 152 can realize the operations of automatic uncovering, automatic scanning, automatic split charging, automatic cover screwing and the like, and the whole input of an original pipe, so that the automatic loading and unloading of samples and consumables and the automatic storage of finished products are realized, the split charging efficiency can be greatly improved, and the efficiency can be improved by 7 times compared with the manual operation efficiency by adopting the sample split charging device 152; the detection flux can be greatly improved, and the manpower and field investment are saved. The full-automatic treatment can avoid the contact of experimenters and virus samples; and meanwhile, a HEPA negative pressure filtering system and an ultraviolet disinfection system are arranged, so that the risk of pollution leakage is effectively prevented. The full-automatic cup separating processing system reduces the manual intervention time, simplifies and standardizes the complex operation, liberates the manpower, reduces the misoperation and the biological pollution and reduces the production cost.

The sample detection unit 17 receives the nucleic acid sample from the sample processing unit 15 by the sample transfer device 16, and the nucleic acid sample processed by the sample processing device 151 in the sample processing unit 15 can be transferred to the sample detection unit 17 by the sample transfer device 16.

In this embodiment, the sample detection unit 17 includes at least one detection device 171.

In this embodiment, the detection device 171 is used to perform an amplification reaction on the extracted RNA sample to obtain a detection result. In particular, in this embodiment, the detection device 171 is a fluorescence quantitative PCR instrument, and the fluorescence quantitative PCR instrument is herein an RT-PCR instrument, wherein the conventional fluorescence quantitative PCR instrument can be compatible with the high-throughput automated sample preparation system and the fully automatic cup processing system.

The information management unit 18 is in communication connection with the reagent preparation unit 12, the sample processing unit 15, the sample detection unit 17 and the environment control unit 11 through a wired or wireless network, so as to realize information management and process management of each link.

Referring to fig. 2, in the present embodiment, the information management unit 18 mainly includes two parts, a first part is sample information management, and a second part is detection flow management.

The first part is sample information management, which mainly aims at the entry management of sample information at each stage, and specifically, information entry devices (such as bar code scanners) are arranged in the reagent preparation unit 12, the sample processing unit 15 and the sample detection unit 17, and are used for collecting sample information at each stage and transmitting the information to the information management unit 18, so that the whole-process informatization management is realized.

The method specifically comprises the following steps:

and in the sample collection stage, the information of the examined person and the inspection sample is imported.

And in the sample preparation stage, unpacking the packages and recording the counting condition of the samples.

And in the sample detection stage, checking the information of the sample tube to be detected and binding the external sample bar code with the internal sample bar code.

And in the sample collection stage, the warehousing of the samples and the inventory management of the ex-warehouse samples.

And in the sample detection stage, the detection result is audited and a detection report is issued.

Specifically, the corresponding bar codes are attached to the containers for containing the samples, and the bar codes of the corresponding samples in the corresponding stages are output to the information management unit 18 through the bar code scanning device, so that the time for data entry and information check of a large number of samples can be reduced, the problem of sample mixing is reduced, the sample storage management and sample tracing are facilitated, meanwhile, the report result can be assisted to be examined, and the time for manual report checking is reduced.

In the second part, the detection process management mainly implements informatization management for each detection process, and specifically, the information management unit 18 is respectively bound with the sample processing device 151, the sample dispensing device 152 and the detection device 171, so as to implement remote monitoring of the above devices.

The method specifically comprises the following steps:

in the sample processing stage, the sample layout and the sample transfer operation into the deep hole plate are monitored.

In the sample processing stage, the relationship between the deep hole plate of the sample dispensing device 152 and the nucleic acid extraction plate of the sample processing device 151 is bound, so that monitoring of the sample to be detected is automatically transferred from the sample dispensing device 152 to the sample processing device 151, and meanwhile, the running state of the nucleic acid extraction device is monitored.

And in the sample detection stage, the relation between the nucleic acid extraction plate of the sample processing device 151 and the RT-PCR plate of the detection device 171 is bound, so that the nucleic acid sample on the nucleic acid extraction plate is transferred to the RT-PCR plate for amplification reaction, and meanwhile, the detection value is automatically captured.

And a sample detection stage, wherein the RT-PCR detection result is confirmed, and the confirmed RT-PCR detection result enters the first sample information management step for checking and detecting the detection result, including outputting.

The system adopts information remote information and process management, can collect and summarize production and equipment information in real time, realizes information visualization and transparence, monitors production capacity, equipment running condition, equipment performance and saturation, and provides data basis for decision making, thereby improving management efficiency and realizing capacity maximization. The information management unit 18 can specifically adopt a laboratory information management unit (huada ZLIMS system) developed by shenzhen hua maozi science and technology limited company to perform information management in the whole detection process, and the huada ZLIMS system serves as an information management platform special for life science instruments and can record experimental process data, monitor the real-time states of the instruments and samples, schedule offline data to be stored, integrate an information analysis platform, start automatic analysis, and form an end-to-end complete solution from sample entry to final report.

The environment control unit 11 is mainly used for controlling the environmental temperature, the environmental pressure, the environmental humidity, the environmental atmosphere, the laboratory water supply and drainage and the laboratory power supply of the whole laboratory.

In the present embodiment, the environment control unit 11 mainly includes a ventilation device 111, a water supply and drainage device 112, a power supply device 113, and a central management station 114.

The ventilation device 111 is used for controlling environmental factors such as experiment temperature, humidity, pressure, atmosphere, etc., the water supply and drainage device 112 is used for supplying and draining water for the whole laboratory, the power supply device 113 is used for supplying power for the whole laboratory, and the central management station 114 is used for managing network communication of the real laboratory.

Referring to fig. 3, the present embodiment provides an integrated intelligent nucleic acid detecting system 200, which includes a housing 21, and an air conditioning and electromechanical chamber 22, a reagent preparing chamber 23, a first dressing chamber 24, a sample processing chamber 25, a buffer chamber 26, a sample detecting chamber 27, and a second dressing chamber 28 sequentially arranged in the housing 21. The reagent preparation unit 12 is provided in the reagent preparation chamber 23, the sample processing unit 15 is provided in the sample processing chamber 25, and the sample detection unit 17 is provided in the sample detection chamber 27.

This box 21 is the box (for example the carriage of vehicle) of cuboid structure approximately, adopts the rain-proof design of excellent heat preservation, and overall structure satisfies engineering structure mechanics requirement, can satisfy the requirement of long distance transportation load and long-time transportation. The floor within the cabinet 21 and the laboratory bench are all sterilizable materials.

Two ends of the box body 21 are provided with a sealed door 211, one sealed door 211 is communicated with the first dressing room 24, and the other sealed door 211 is communicated with the second dressing room 28.

The first dressing room 24 is located between the reagent preparation room 23 and the sample processing room 25, and a first decontamination basin 241 is provided therein for an operator to enter the laboratory for cleaning. Two airtight doors 211 are provided in the first dressing room 24 to allow access to the reagent preparation room 23 and the sample processing room 25, respectively.

The second changing room 28 is located at the side of the sample testing room 27 far from the sample processing room 25, and the second changing room 28 is communicated with the sample testing room 27 through another two sealed doors 211 for performing the corresponding washing operation when the operator leaves the laboratory. An autoclave 281 and a second decontamination basin 282 are arranged in the second dressing room 28, the autoclave 281 is used for autoclave sterilization of the personal laboratory supplies, and the second decontamination basin 282 is used for personal decontamination of the operators.

The reagent preparation unit 12 is disposed in the reagent preparation chamber 23, the clean bench 122 is disposed on one side of the reagent preparation chamber 23, the storage device 121 is disposed on the same side, the storage device 121 is used for storing relevant reagents for detection, and the clean bench 122 is used for reagent preparation operation. The reagent transfer device 14 is disposed on the side opposite to the storage device 121, wherein the reagent transfer device 14 is used for transferring the reagent into the sample processing chamber 25, a transfer channel and windows disposed on the two sides of the transfer channel are disposed in the reagent transfer device 14, wherein an illuminating lamp and an ultraviolet sterilizer are disposed in the transfer channel, and the reagent transfer device 14 can conveniently and quickly transfer the reagent into the sample processing chamber 25.

Referring to fig. 3 and 4, the sample processing unit 15 is disposed in the sample processing chamber 25, and the sample processing device 151 can be, but not limited to, an automatic sample preparation system (MGISP-100B, 192/8 h-576/24 h theoretical flux), an automatic high-flux sample preparation system (model: MGISP-960, 192/8 h-576/24 h theoretical flux) or a nucleic acid extractor (model: NE 384) developed by shenzhen hua maozheng science and technology limited.

Wherein the sample processing chamber 25 communicates with the outside through the sample transfer device 13 and receives a sample to be tested. The sample transfer device 13 is provided with a transfer channel and window bodies arranged at two sides of the transfer channel, wherein the transfer channel is internally provided with an illuminating lamp and an ultraviolet sterilizer, and the sample transfer device 13 can conveniently and rapidly transfer the sample to the sample processing chamber 25.

Referring to fig. 5, in another embodiment, the sample processing unit 15 further includes a sample dispensing device 152, wherein the sample dispensing device 152 is disposed at one side of the sample processing chamber 25 and is used for dispensing the sample to be detected, and a high-throughput full-automatic cup dispensing processing system (MGISTP-7000, 192 cases/40 min) developed by shenzhen hua wisdom manufacturing science and technology limited company can be specifically used, but the system has a large throughput, can significantly improve the detection efficiency, and is helpful for realizing high-throughput detection in a laboratory.

Referring to fig. 3 to 5, the sample detecting unit 17 is disposed in the sample detecting chamber 27, the sample detecting chamber 27 is communicated with the sample processing chamber 25 through the sample transferring device 16, and the detecting device 171 is used for PCR amplification of the nucleic acid sample, wherein a plurality of detecting devices 171 (e.g., RT-PCR instruments) are disposed in the sample detecting chamber 27, so as to improve the detection efficiency.

In this embodiment, the airtight doors 211 are all dedicated airtight doors, and are equipped with a hollow glass observation window, a door lock, a handle, a door closer, a lifting seal, an electronic interlock, and the like.

Referring to fig. 1, in addition, the integrated intelligent nucleic acid detecting system 200 further includes a ventilation device 111, wherein, in order to ensure the safety of the experiment and the personnel, the whole production line adopts a full fresh air and full exhaust direct current air conditioning system, the ventilation frequency is greater than 12 times/h, the cleaning grade is one hundred thousand grade, the indoor temperature is 18-26 ℃, the humidity is less than 75-75%, and the noise is less than 60dB. The pressure in the chamber is integral negative pressure and is unidirectional airflow; the air feeding and exhausting mode is upward feeding and downward returning; the air port is reasonable in position design, and air flow of the biological safety cabinet is not influenced. The laboratory air outlet is provided with a P3 laboratory-level double-in-situ efficient exhaust filtering device, the side wall is installed, and the upper part of the air outlet is provided with an electric biological airtight valve to prevent indoor polluted air from being exhausted outdoors.

Referring to fig. 1, the integrated intelligent nucleic acid detecting system 200 further includes a water supply and drainage device 112, which can be connected to domestic tap water and hot water, and is provided with a clean tap water outlet tank. The protective clothing is worn with a hand sterilizer, and the first and

second dressing rooms

24 and 28 are provided with stainless steel hand washing basins, automatic induction taps, soap dispenser, spray hand sterilizer and hand dryer. PP experiment water tanks are arranged in the sample processing chamber 25 and the sample detection chamber 27, a single-port cold-hot water experiment faucet and a double-port eye washer are arranged, and the upper water and the lower water in the two chambers are closed and are not used when a new crown epidemic situation is processed. And floor drains are not arranged in all rooms of the laboratory. The water drained is used for washing hands, and a sewage treatment device is not arranged in the laboratory for storage, disinfection and sterilization and is directly drained into a nearby sewage well. A small-sized wastewater treatment system can be optionally installed.

Referring to fig. 1, the integrated intelligent nucleic acid detecting system 200 further includes a power supply device 113, a 380V-63A industrial connector (a male and female set) is reserved on the side of the casing of the box 21 for power distribution connection, and a 380V-40KW power supply is led to the laboratory electromechanical cabin from the owner. The illumination and entrance guard security device is provided with a UPS power supply, and the power supply time is not less than 15min. The designed illumination is considered in each experimental area as follows: the air conditioning and electromechanical room 22 is 100lx, the first changing room 24, the second changing room 28 and the buffer room 26 are 200lx, and the reagent preparation room 23, the sample processing room 25 and the sample detection room 27 are 300lx. Except for normal illumination, all the laboratory working rooms are provided with ultraviolet germicidal lamps, and the first dressing room 24 and the second dressing room 28 are provided with time delay switches for separate control. All lamps and lanterns all adopt clean lamps and lanterns, and all light sources all adopt the LED light source. The light switch is controlled individually for each room.

Referring to fig. 1, the integrated intelligent nucleic acid detecting system 200 further includes a central management station 114, the air-conditioning and electromechanical room 22 is provided with four external network cable interfaces, one for telephone, two for network, one for security reservation, 8 switches in the warehouse, and information points in the laboratory to ensure smooth transmission of experimental data information. The hospital is responsible for the access of external weak wires, and a 5G wireless communication card can be optionally matched. All the airtight doors 211 are provided with access control devices, the room is provided with a monitoring camera, and a memory is arranged in the weak current box. An independent automatic control system is arranged in the air conditioner and electromechanical room 22, and is used for configuration, programming, fault diagnosis and the like of the system, centralized operation is realized for managers, effective and accurate data are provided, and all equipment in a laboratory forms effective and reasonable operation order so as to achieve effective energy conservation. A touch operation screen is arranged on the wall of the first dressing room 24, and pressure difference sensors with display are arranged in the sample processing room 25 and the sample detection room 27 of the second dressing room 28.

The application provides an integrated form intelligence nucleic acid detecting system 100, can make up the multiunit laboratory according to the user demand of difference and assemble, specifically, this application provides three kinds of design, is high throughput scheme, basic scheme and emergent scheme respectively.

Referring to fig. 3, the integrated intelligent nucleic acid detecting system 200 according to the present embodiment is a single-compartment basic scheme: theoretical flux: 700/8h to 2100/24h, and the sample processing chamber 25 was equipped with a sample processing device 151 using a high-throughput automated sample preparation system (model: MGISP-960, MGISP-100B) and a nucleic acid extractor (model: NE 384), the laboratory management system employed was the ZLIMS system manufactured by huada.

Referring to fig. 4, the integrated intelligent nucleic acid detecting system 300 according to the present embodiment is a dual-chamber high-throughput scheme: the two integrated intelligent nucleic acid detection systems 200 provided by the basic scheme are combined to realize high-flux detection, and the two groups are communicated with each other through the sample transfer device 13 to realize interaction of samples to be detected, wherein the two groups of the integrated intelligent nucleic acid detection systems 200 are communicated with each other through the two sample processing chambers 25.

Referring to fig. 5, the integrated intelligent nucleic acid detecting system 400 according to the present embodiment is a single-chamber high-throughput scheme: theoretical flux: 192/8h to 576/24h, the sample processing chamber 25 was equipped with a sample dispensing device 152 (model: MGISTP-7000) and a sample processing device 151 (model: MGISP-100B), and the laboratory management system used was the ZLIMS system manufactured by huada.

The application provides an integrated form intelligence nucleic acid detecting system 100 can be along with the car quick travel, satisfies the nucleic acid testing of anytime and anywhere, examines the measurement 1000 people daily, and one-stop form nucleic acid detects and can shorten virus nucleic acid extraction time by a wide margin, satisfies medical staff and draws the demand of in-process nimble, quick automation and safe prevention and control to the flux at the virus nucleic acid. The main application scenarios include: customs and entry ports in various regions, airports, train stations, long-distance bus stations, etc., schools, exhibition halls, shopping malls, commercial buildings, governments and businesses for external assistance.

The integrated intelligent nucleic acid detection system 100 provided by the application can be applied to detection of new coronavirus, and accords with technical guidelines (third edition) for laboratory detection of pneumonia caused by infection of new coronavirus, a clinical gene amplification inspection laboratory management method 2010 of medical institutions, GB11489-2008 laboratory biosafety general requirements, WS-233-2017 microorganisms, biomedical laboratory biosafety general criteria, GB50849-2014: the building design specification of infectious disease hospitals, T-CECS 662-2020 building technical standard of medical biosafety secondary laboratory, GB-50346-2011 building technical specification of biosafety laboratories, GB-27421-2015 mobile laboratory biosafety requirements and other industrial standards.

In addition, it is obvious to those skilled in the art that other various corresponding changes and modifications can be made according to the technical idea of the present application, and all such changes and modifications should fall within the protective scope of the claims of the present application.

Claims

An integrated intelligent nucleic acid detection system, comprising:

the sample processing unit is used for extracting a nucleic acid sample in a sample to be detected;

the sample detection unit is used for detecting the nucleic acid sample to obtain a detection result; and

and the information management unit is used for acquiring and outputting the detection result.
The integrated intelligent nucleic acid detection system according to claim 1, wherein the sample processing unit comprises at least one sample processing device for extracting nucleic acid samples from the sample to be detected.
The integrated intelligent nucleic acid detection system of claim 2, wherein the sample processing device is a nucleic acid extractor.
The integrated intelligent nucleic acid detecting system according to claim 2, wherein the sample processing unit further comprises at least one sample dispensing device for dispensing the sample to be detected.
The integrated intelligent nucleic acid detecting system according to claim 1, wherein the sample detecting unit comprises at least one detecting device, and the detecting device is an RT-PCR detector.
The integrated intelligent nucleic acid detecting system according to claim 1, wherein the integrated intelligent nucleic acid detecting system comprises at least one laboratory, each laboratory comprises a housing, and a sample processing chamber and a sample detecting chamber disposed in the housing, the sample processing unit is disposed in the sample processing chamber, and the sample detecting unit is disposed in the sample detecting chamber.
The integrated intelligent nucleic acid detecting system according to claim 6, wherein the laboratory further comprises a sample transfer device and a sample detection transfer device, the sample processing chamber receives the sample to be detected through the sample transfer device, and the sample detection chamber is communicated with the sample processing chamber through the sample detection transfer device.
The integrated intelligent nucleic acid testing system of claim 6, wherein said laboratory further comprises a reagent preparation chamber having a reagent preparation unit disposed therein for providing a testing reagent.
The integrated intelligent nucleic acid detection system of claim 8, wherein the laboratory further comprises a reagent delivery device through which the sample processing chamber communicates with the reagent preparation chamber.
The integrated intelligent nucleic acid detection system of claim 7, wherein the integrated intelligent nucleic acid detection system comprises two sets of laboratories, and wherein the two sets of laboratories are in communication with each other via another sample transfer device.