CN212453800U

CN212453800U - Unidirectional moving-line movable pathogen nucleic acid detection laboratory system

Info

Publication number: CN212453800U
Application number: CN202021341378.5U
Authority: CN
Inventors: 谢孝民; 李丹; 谢正华; 熊磊; 陈才夫; 孙祎
Original assignee: Shanghai 3D Medicines Co Ltd
Current assignee: Shanghai 3D Medicines Co Ltd
Priority date: 2020-07-09
Filing date: 2020-07-09
Publication date: 2021-02-02
Anticipated expiration: 2030-07-09

Abstract

The utility model relates to a one-way moving line mobile pathogen nucleic acid detection laboratory system, which realizes the isolation requirement among three steps in the nucleic acid detection process by independently arranging three main steps of reagent storage and preparation, sample preparation, amplification and product analysis related to the nucleic acid detection process in three mobile structures, designs strict one-way moving lines of personnel and articles according to the national standard, and accords with the safety requirement; meanwhile, the three movable structures can be quickly moved, deployed to any required position and unfolded to form a pathogen nucleic acid detection laboratory with complete functions, so that the detection is performed on the spot at the key prevention and control positions, and the concentrated transfer of high risk groups is avoided.

Description

Unidirectional moving-line movable pathogen nucleic acid detection laboratory system

Technical Field

The utility model relates to a pathogen detection equipment technical field especially relates to a portable pathogen nucleic acid detection laboratory system of one-way acting line.

Background

The novel coronary pathogen is an airborne pathogen with the characteristics of strong infectivity and strong pathogenicity, often presents explosive diffusion infection in community people, and poses a very serious threat to the life health of people.

Because no specific medicine exists for pneumonia and other diseases caused by the novel coronary pathogens at present, the early discovery and isolation of infected people become the most effective means for preventing and treating the novel coronary pathogens. The most commonly used nucleic acid detection method at present is a screening means with high public accuracy and reliable result, but the nucleic acid detection generally needs to be operated in a detection laboratory meeting the requirements, so that the number of the detection laboratories is not enough to meet huge detection requirements, the nucleic acid detection efficiency is severely restricted, and the optimal treatment time is delayed in the process of waiting for the nucleic acid detection by a plurality of patients, so that the disease condition is aggravated. Therefore, there is an urgent need for a mobile nucleic acid detection laboratory system that meets biosafety laboratory standards.

SUMMERY OF THE UTILITY MODEL

For solving prior art's not enough, the utility model provides a portable pathogen nucleic acid detection laboratory system of one-way moving wire, through the reagent preparation that involves with the nucleic acid testing process, sample treatment, the three main step of sample analysis is independent setting respectively in three movable structure, realize the isolation requirement between the three step among the nucleic acid testing process, three movable structure can the quick travel deploy to arbitrary demand position and expand and form a pathogen nucleic acid detection laboratory that has complete function simultaneously, realize that key prevention and control position carries out the detection on the spot, avoid high risk group to concentrate the transfer.

In order to achieve the above object, the utility model discloses the technical scheme who adopts includes:

a unidirectional kinetic line mobile pathogen nucleic acid detection laboratory system comprising any one or a combination of any plurality of reagent storage and preparation mobile structures, specimen preparation mobile structures and amplification and product analysis mobile structures isolated from each other and independent of each other, and wherein the number of any single kind of mobile structure in the system is one or more;

the reagent storage and preparation movable structure comprises a first inlet buffer room, a reagent preparation room maintaining a positive pressure environment, a first sewage disposal room and a first outlet buffer room which are connected in sequence in an airtight manner, a one-way airtight personnel passage relative to the external environment is formed, and the side wall of the reagent preparation room is provided with at least one airtight transfer window;

the movable specimen preparation structure comprises a second inlet buffer room, a specimen processing room maintaining a negative pressure environment, a second sewage disposal room and a second outlet buffer room which are sequentially connected in an airtight manner to form a one-way airtight personnel channel relative to the external environment, and the side wall of the specimen processing room is at least provided with two mutually independent airtight transfer windows;

the amplification and product analysis movable structure comprises a third inlet buffer room, an amplification analysis room maintaining a negative pressure environment, a third pollutant disposal room and a third outlet buffer room which are connected in an airtight mode in sequence, a one-way airtight personnel channel relative to an external environment is formed, and at least one airtight transfer window is arranged on the side wall of the amplification analysis room.

Further, the airtight transfer windows arranged on the reagent storage and preparation movable structure, the specimen preparation movable structure and the amplification and analysis movable structure are communicated through the detachable airtight connected conveying pipelines for airtight transfer of articles.

Further, a positive pressure environment is maintained between the first inlet buffer room and the first outlet buffer room, and the positive pressure environment pressure value between the first inlet buffer room and the first outlet buffer room is lower than the positive pressure environment pressure value of the reagent preparation chamber; and the negative pressure environment is maintained between the second inlet buffer and the second outlet buffer and between the third inlet buffer and the third outlet buffer, the negative pressure environment pressure value between the second inlet buffer and the second outlet buffer is higher than that of the sample processing chamber, and the negative pressure environment pressure value between the third inlet buffer and the third outlet buffer is higher than that of the amplification analysis chamber.

Further, the first sewage disposal room maintains a positive pressure environment, and the positive pressure environment pressure value of the first sewage disposal room is lower than the positive pressure environment pressure value of the reagent preparation room and is simultaneously higher than the positive pressure environment pressure value of the first outlet buffer room; the second sewage disposal room maintains a negative pressure environment, and the negative pressure environment pressure value of the second sewage disposal room is higher than that of the sample processing chamber and lower than that of the second outlet buffer room; and a negative pressure environment is maintained in the third sewage disposal room, and the negative pressure environment pressure value of the third sewage disposal room is higher than that of the amplification analysis chamber and lower than that of the third outlet buffer room.

Further, the pressure value of the reagent preparation chamber is 30Pa, the pressure values of the first inlet buffer chamber and the first outlet buffer chamber are both 10Pa, and the pressure value of the first sewage disposal chamber is 20 Pa; the pressure value of the sample processing chamber is-30 Pa, the pressure values of the second inlet buffer chamber and the second outlet buffer chamber are both-10 Pa, and the pressure value of the second sewage disposal chamber is-20 Pa; the pressure value of the amplification analysis chamber is-25 Pa, the pressure values of the third inlet buffer chamber and the third outlet buffer chamber are both-10 Pa, and the pressure value of the third pollutant disposal chamber is-15 Pa.

Further, the reagent storage and preparation movable structure, the specimen preparation movable structure and the amplification and product analysis movable structure are respectively connected with a mains supply through detachable cables for power supply.

Further, the amplification and product analysis movable structure further comprises a generator set; the power generation unit is connected to the reagent storage and preparation mobile structure, the specimen preparation mobile structure and/or the amplification and product analysis mobile structure via detachable cables and provides electrical power.

Further, the generator set is movably received in the third exit buffer compartment and moved out of the third exit buffer compartment to reposition the generator set when power is supplied.

Further, the mobile platform provided with the movable structure comprises a van, a box trailer or a container with an airtight isolated cab.

Further, the reagent storage and preparation mobile structure, the specimen preparation mobile structure, and/or the amplification and product analysis mobile structure may be disposed in any combination on one mobile platform, or may be disposed in any combination on a plurality of mobile platforms independent of each other.

The utility model has the advantages that:

adopt portable pathogen nucleic acid detection laboratory system of one-way acting line bears the different steps of carrying out among the nucleic acid testing process respectively through the portable structure who adopts three mutually independent isolation, can the different operational environment of adjustment of pertinence: for the reagent preparation step with high requirement on environment cleanliness, a positive pressure environment is adopted to ensure that the internal air is not polluted by the outside; for the sample processing step involving pathogen contact, a negative pressure environment is adopted to prevent the pathogen from accidentally leaking, ensure safety and prevent secondary transmission. On this basis, but movable structure that three mutual independence kept apart all satisfies personnel, the one-way moving line design of article, forms complete airtight isolation protection, avoids the pollutant diffusion, accords with the national mandatory standard requirement completely. By applying the one-way moving-line movable pathogen nucleic acid detection laboratory system, the nucleic acid detection capacity can be rapidly adjusted to the hotspot demand area, the detection capacity is rapidly formed, time-consuming laboratory construction is not needed, and the sudden detection capacity shortage of a specific area is compensated by the function of rapid cross-area allocation of the existing nucleic acid detection capacity; meanwhile, the mobile detection capability can effectively avoid concentrated transportation and aggregation of a large number of high risk groups, and is beneficial to the orderly development of large-scale nucleic acid detection matters.

Drawings

Fig. 1 is a schematic view of a first embodiment of the present invention.

Fig. 2 is a schematic view of a second embodiment of the present invention.

Description of the figure numbering: 1-reagent storage and preparation mobile structure, 11-reagent preparation chamber, 12-first inlet buffer chamber, 13-first waste disposal chamber, 14-first outlet buffer chamber, 2-specimen preparation mobile structure, 21-sample processing chamber, 22-second inlet buffer chamber, 23-second waste disposal chamber, 24-second outlet buffer chamber, 3-amplification and product analysis mobile structure, 31-amplification analysis chamber, 32-third inlet buffer chamber, 33-third waste disposal chamber, 34-third outlet buffer chamber, 343-generator set, 4-airtight transmission window, 5-mains supply, 6-detachable cable, 7-transmission pipeline.

Detailed Description

For a clearer understanding of the present invention, reference will be made to the following detailed description of the embodiments with reference to the accompanying drawings.

At present, pathogen nucleic acid detection laboratories are generally arranged in fixed facilities such as hospitals or related research institutes, and detection samples need to be collected and then are intensively sent to the detection laboratories for detection. Such a laboratory setting mode is inconvenient for short-term expansion of detection capability and reasonable configuration of detection capability, and often results in the situation that detection capability of key areas is seriously insufficient, and simultaneously detection capability of non-hotspot areas is greatly idle and wasted, which does not meet practical requirements of epidemic situation resistance.

Some movable laboratory designs are also proposed in the prior art, but most of the movable laboratory designs adopt an integrated structure designed for a large bus chassis, which is not beneficial to mutual isolation among various areas and has poor detection applicability to pathogens with strong infectivity.

As shown in FIG. 1, the laboratory system for detecting a pathogen's nucleic acid with one-way moving line according to the first embodiment of the present invention preferably comprises three parts, i.e., a reagent storage and preparation movable structure 1, a specimen preparation movable structure 2, and an amplification and product analysis movable structure 3, which are isolated from each other and independent from each other. The reagent storage and preparation movable structure 1 corresponds to a reagent storage and preparation area in a nucleic acid detection laboratory and is responsible for preparing a stored reagent, subpackaging the reagent and preparing an amplification reaction mixed solution and storing and preparing consumables such as a centrifuge tube, a suction head and the like; the sample preparation movable structure 2 corresponds to a sample preparation area in a nucleic acid detection laboratory and is responsible for extracting, storing and adding nucleic acid (RNA and DNA) into an amplification reaction tube, particularly, the sample preparation movable structure 2 can relate to the operation of clinical samples and can meet the requirements of biological safety secondary laboratory protection equipment, personal protection and operation specifications; the movable amplification and product analysis structure 3 corresponds to an amplification area and an amplification product analysis area in a nucleic acid detection laboratory and is responsible for cDNA synthesis, DNA amplification and detection, and further analysis and determination of amplified fragments, such as hybridization, enzyme digestion electrophoresis, denaturing high performance liquid analysis, sequencing and the like. The reagent storing and preparing movable structure 1 comprises a first inlet buffer room 12, a reagent preparing room 11 maintaining a positive pressure environment, a first sewage disposal room 13 and a first outlet buffer room 14 which are connected in sequence in an airtight way, a one-way airtight personnel passage relative to the external environment is formed, and at least one airtight transfer window 4 is arranged on the side wall of the reagent preparing room 11. Functional components such as a nucleic acid detection reagent preparing component, a nucleic acid detection reagent dispensing component, and a nucleic acid detection reagent storing component may be provided in the reagent preparation chamber 11 as necessary. The movable specimen preparation structure 2 comprises a second inlet buffer room 22, a sample processing room 21 maintaining a negative pressure environment, a second sewage disposal room 23 and a second outlet buffer room 24 which are connected in sequence in an airtight manner, a one-way airtight personnel channel relative to an external environment is formed, and at least two mutually independent airtight transfer windows 4 are arranged on the side wall of the sample processing room 21. According to the requirement, the sample processing chamber 21 can be provided with functional components such as a sample inactivation component, a sample nucleic acid extraction component, a nucleic acid sample adding component and a to-be-detected reagent sealing component. The movable amplification and product analysis structure 3 comprises a third inlet buffer room 32, an amplification and analysis chamber 31 maintaining a negative pressure environment, a third waste disposal room 33 and a third outlet buffer room 34 which are connected in sequence in an airtight manner, a one-way airtight personnel passage relative to the external environment is formed, at least one airtight transfer window 4 is arranged on the side wall of the amplification and analysis chamber 31, and a movable generator set 343 is preferably accommodated in the third outlet buffer room 34.

The arrangement of the first waste disposal room 13, the second waste disposal room 23 and the third waste disposal room 33 enables an experimenter to have an additional buffer space before leaving the polluted area, so that waste can be treated in the waste disposal rooms and then leaves through the outlet buffer room, and secondary pollution caused by the waste generated in the nucleic acid detection process is avoided. The first waste disposal room 13, the second waste disposal room 23 and the third waste disposal room 33 may be preferably provided with a harmless treatment facility such as a high temperature and high pressure treatment facility, an incineration facility and the like, and an airtight temporary waste storage facility and the like.

The three movable structures can preferably move respectively without interference and can be rapidly unfolded at a required place to form a nucleic acid detection laboratory. A first embodiment of the reagent storage and preparation mobile structure 1, the specimen preparation mobile structure 2 and the amplification and product analysis mobile structure 3 deployed at a designated site is shown in fig. 1. In the first embodiment, the reagent storage and preparation mobile structure 1, the specimen preparation mobile structure 2, and the amplification and product analysis mobile structure 3 are developed side by side in this order to form a natural buffer region; the right side of the reagent storage and preparation mobile structure 1 is provided with an airtight transfer window 4 facing the specimen preparation mobile structure 2; the specimen preparation movable structure 2 is provided with one airtight transfer window 4 on each of the left and right sides, wherein the airtight transfer window 4 provided on the left side faces the reagent storage and preparation movable structure 1 for facilitating the transfer of the article with the reagent storage and preparation movable structure 1, and the airtight transfer window 4 provided on the right side faces the amplification and product analysis movable structure 3 and is connected to the airtight transfer windows 4 through the transfer pipe 7 to form a completely airtight article transfer passage. In embodiment 1, the reagent storage and preparation mobile structure 1, the specimen preparation mobile structure 2, and the amplification and product analysis mobile structure 3 are all connected to a commercial power supply 5 through a detachable cable 6.

As shown in fig. 2, the second embodiment of the present invention is also similar to the first embodiment and is developed for a specific site, but different from the first embodiment, in order to cope with the situation that the utility power supply 5 is not conveniently accessed, in the second embodiment, the reagent storage and preparation movable structure 1, the specimen preparation movable structure 2 and the amplification and product analysis movable structure 3 are powered by the generator set 343 provided on the amplification and product analysis movable structure 3, and in the case of using the generator set 343 for power supply, the single connection length of the detachable cable 6 should not be more than 10 meters to ensure the power supply safety. The genset 343 is housed within the third exit buffer compartment 34 without being enabled; move to the external environment during the start-up and freely select the position of placing of generating set 343 according to three movable structure's the position of setting, conveniently can dismantle the cable arrangement and connect. The generator set 343 may preferably use a power supply device capable of continuously outputting a stable voltage, such as a diesel generator.

Of course, the power generator unit 343 may also be preferably directly connected and powered to the amplification and product analysis movable structure 3 only through the detachable cable 6, while the amplification and product analysis movable structure 3 is further connected in series to the specimen preparation movable structure 2 and the reagent storage and preparation movable structure through the detachable cable 6 to form a series power supply structure. Due to the adoption of the series power supply structure which is connected in sequence, the limitation on the placement position of the generator set 343 is further reduced, and the long-distance power supply of the movable structure can be realized without considering the length limitation of the single detachable cable 6 which is connected with the generator set 343.

The reagent storage and preparation movable structure 1, the specimen preparation movable structure 2 and the amplification and product analysis movable structure 3 of the utility model can be combined together as shown in the embodiment 1 and the embodiment 2, and can be respectively used alone or combined in any two ways; meanwhile, in order to balance the processing capacities of the different steps, a plurality of mobile structures of the same kind may be included in one system, and for example, two reagent storage and preparation mobile structures 1, one specimen preparation mobile structure 2, and two amplification and product analysis mobile structures 3 may be provided in one system to sufficiently exert the processing capacities of the respective mobile structures.

Airtight pass-through box 4 can guarantee the structure gas tightness in the article transmission process through opening first airtight door and second airtight door in proper order including the first airtight door and the second airtight door that open simultaneously. Of course other airtight pass-through box designs can also be used in the present invention to achieve the same technical effect. Pipeline 7 can be preferably support the airtight pipeline of slope of dismantling the connection, make article can rely on self gravity to remove in the pipeline, also can choose for use to possess the airtight pipeline of initiative transport power supply.

In order to further satisfy laboratory safety needs, especially will stop the possibility that airborne virus pollutes external environment, but movable structure in different positions adopted different ambient pressure value, form the pressure gradient design, guarantee that the contaminated area air can not leak to external environment. Preferably, the first inlet buffer room 12 and the first outlet buffer room 14 maintain a positive pressure environment, and the positive pressure environment pressure value of the first inlet buffer room 12 and the first outlet buffer room 14 is lower than the positive pressure environment pressure value of the reagent preparation chamber 11; the first waste disposal room 13 maintains a positive pressure environment, and a positive pressure environmental pressure value of the first waste disposal room 13 is lower than a positive pressure environmental pressure value of the reagent preparation chamber 11 and higher than a positive pressure environmental pressure value of the first outlet buffer room 14; the second inlet buffer room 22, the second outlet buffer room 24, the third inlet buffer room 32 and the third outlet buffer room 34 are all maintained in a negative pressure environment, the negative pressure environment pressure value of the second inlet buffer room 22 and the second outlet buffer room 24 is higher than the negative pressure environment pressure value of the sample processing chamber 21, and the negative pressure environment pressure value of the third inlet buffer room 32 and the third outlet buffer room 34 is higher than the negative pressure environment pressure value of the amplification analysis chamber 31; the second waste disposal room 23 maintains a negative pressure environment, and the negative pressure environment pressure value of the second waste disposal room 23 is higher than the negative pressure environment pressure value of the sample processing chamber 21 and lower than the negative pressure environment pressure value of the second outlet buffer room 24; the third waste disposal chamber 33 maintains a negative pressure environment, and the negative pressure environment pressure value of the third waste disposal chamber 33 is higher than the negative pressure environment pressure value of the amplification analysis chamber 31 and lower than the negative pressure environment pressure value of the third outlet buffer chamber 34. Specifically, each of the above regions may be set to achieve a better protection effect by using the following environmental pressures: the pressure value of the reagent preparation chamber 11 is 30Pa, the pressure values of the first inlet buffer room 12 and the first outlet buffer room 14 are both 10Pa, and the pressure value of the first sewage disposal room 13 is 20 Pa; the pressure value of the sample processing chamber 21 is-30 Pa, the pressure values of the second inlet buffer room 22 and the second outlet buffer room 24 are both-10 Pa, and the pressure value of the second sewage disposal room 23 is-20 Pa; the pressure value of the amplification analysis chamber 31 is-25 Pa, the pressure values of the third inlet buffer chamber 32 and the third outlet buffer chamber 34 are both-10 Pa, and the pressure value of the third dirt disposal chamber 33 is-15 Pa.

Preferably, the connecting doors adjacent to each region can be rotary airtight doors which are opened by rotating towards the side with higher environmental pressure, so that people can feel appropriate resistance formed by larger environmental pressure when opening the connecting doors, and meanwhile, the connecting doors can be pushed to rotate and close by utilizing the pressure difference between the environments.

The first entrance buffer room 12 and the first exit buffer room 14, the second entrance buffer room 22 and the second exit buffer room 24, and the third entrance buffer room 32 and the third exit buffer room 34 may preferably provide a rest function for detecting people, such as a washroom, a bathroom, etc., in addition to providing necessary functions of changing clothes and sterilizing for people.

The above description is only for the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A unidirectional kinetic line mobile pathogen nucleic acid detection laboratory system comprising any one or a combination of any plurality of reagent storage and preparation mobile structures, specimen preparation mobile structures and amplification and product analysis mobile structures isolated from each other and independent of each other, and wherein the number of any single kind of mobile structure in the system is one or more;

2. The system of claim 1, wherein the airtight transfer windows provided by the reagent storage and preparation mobile structure, the specimen preparation mobile structure, and the amplification and analysis mobile structure communicate with each other via a detachable airtight connected transfer conduit for airtight transfer of articles.

3. The system of claim 1, wherein the first inlet buffer and the first outlet buffer maintain a positive pressure environment therebetween, and a positive pressure environment pressure value between the first inlet buffer and the first outlet buffer is lower than the reagent preparation chamber positive pressure environment pressure value; and the negative pressure environment is maintained between the second inlet buffer and the second outlet buffer and between the third inlet buffer and the third outlet buffer, the negative pressure environment pressure value between the second inlet buffer and the second outlet buffer is higher than that of the sample processing chamber, and the negative pressure environment pressure value between the third inlet buffer and the third outlet buffer is higher than that of the amplification analysis chamber.

4. The system of claim 3, wherein the first waste disposal room maintains a positive pressure environment, and a positive pressure ambient pressure value of the first waste disposal room is lower than a positive pressure ambient pressure value of the reagent preparation chamber and higher than a positive pressure ambient pressure value of the first outlet buffer room; the second sewage disposal room maintains a negative pressure environment, and the negative pressure environment pressure value of the second sewage disposal room is higher than that of the sample processing chamber and lower than that of the second outlet buffer room; and a negative pressure environment is maintained in the third sewage disposal room, and the negative pressure environment pressure value of the third sewage disposal room is higher than that of the amplification analysis chamber and lower than that of the third outlet buffer room.

5. The system of claim 4, wherein the pressure value of the reagent preparation chamber is 30Pa, the pressure value of the first inlet buffer and the first outlet buffer is 10Pa, and the pressure value of the first waste disposal chamber is 20 Pa; the pressure value of the sample processing chamber is-30 Pa, the pressure values of the second inlet buffer chamber and the second outlet buffer chamber are both-10 Pa, and the pressure value of the second sewage disposal chamber is-20 Pa; the pressure value of the amplification analysis chamber is-25 Pa, the pressure values of the third inlet buffer chamber and the third outlet buffer chamber are both-10 Pa, and the pressure value of the third pollutant disposal chamber is-15 Pa.

6. The system of claim 1, wherein the reagent storage and preparation mobile structure, the specimen preparation mobile structure, and the amplification and product analysis mobile structure are each separately mains powered by a detachable cable connection.

7. The system of claim 1, wherein the amplification and product analysis mobile structure further comprises a power generator set; the power generation unit is connected to the reagent storage and preparation mobile structure, the specimen preparation mobile structure and/or the amplification and product analysis mobile structure via detachable cables and provides electrical power.

8. The system of claim 7, wherein the generator set is removably received within the third exit buffer compartment and repositioned by moving out of the third exit buffer compartment while providing power.

9. A system according to any one of claims 1 to 8, wherein the mobile platform on which the moveable structure is provided comprises a van, van trailer or container with a hermetically sealed cab.

10. The system of claim 9, wherein the reagent storage and preparation mobile structures, the specimen preparation mobile structures, and/or the amplification and product analysis mobile structures are disposed in any combination on one mobile platform, or in any combination on a plurality of independent mobile platforms.