CN2426984Y

CN2426984Y - Prawn pathogeny detecting reagent box

Info

Publication number: CN2426984Y
Application number: CN 00215068
Authority: CN
Inventors: 史成银; 杨冰; 黄倢; 宋晓玲
Original assignee: Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Current assignee: Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority date: 2000-06-07
Filing date: 2000-06-07
Publication date: 2001-04-18
Anticipated expiration: 2010-06-07

Abstract

The utility model relates to a prawn pathogeny detecting reagent box, which is composed of a rectangular outer box cover and an inner bottom box which is contained in the rectangular outer box cover with sliding fit. A reagent bottle taking the length of the box between 1 / 2-3 / 5 and a tube hole bracket are arranged in the inner bottom box. The plane of the bracket is arranged at a proper position which is below a box opening of the inner bottom box, and a bottle and tube holes are arranged on the plane in order. The bracket is plugged tightly at one end of the inner bottom box, and a bagging plate and prepared bottles and tubes are arranged in a space between a vertical bracket wall at the other end of the bracket and a wide vertical surface at the other end of the inner bottom box. The bottles and the tube holes are arranged on the top plane of an outer box cover covered on the inner bottom box in order, and box pasting films are covered on the holes. The box can be used for detecting prawn pathogeny with the advantages of accuracy, sensitivity, quickness and safety.

Description

Prawn cause of disease detection kit

The utility model relates to the improvement of bioanalysis checkout equipment, specifically is a kind of prawn cause of disease detection kit, and it belongs to the bioengineering technical field of analysis and detection.

In the prawn culturing engineering, the popular of prawn ' s virus outburst disease caused tremendous loss to prawn culturing.Have the high characteristics of detection sensitivity though conventional PCR instrument detects, its instrument and required reagent all compare expensive, and its operation requires height, careless slightlyly false positive will occur.Therefore the PCR method only is applicable to the research application, and is unsuitable for producing application.Also has a kind of TE decoration method.This method can detect multiple virus, and detection time is short, is applicable to field condition.But weak point is arranged also: (1) can not make definite location, troublesome poeration to institutional framework.(2) the prawn state of an illness initial stage difficulty detect, detected day after the fairly and infect the middle and later periods to characteristic of disease, certain limitation is arranged.Above-mentioned two methods are subjected to the region restriction, and sample carries inconvenience, can not adapt to the demand that prawn produces.

The purpose that the utility model relates to is that a kind of novel prawn cause of disease detection kit will be provided.This box is simple in structure, and reagent bottle, pipe are placed stable, are not shifted, and do not mix, and be safe in utilization, and operation is accurately sensitive, is applicable to that advanced studies chamber and basic unit laboratory detect the prawn cause of disease effectively, instructs prevention and cure of shrimp disease work.

Task of the present utility model is finished by following technical scheme, developed a kind of prawn cause of disease detection kit, it is that the outer lid of cuboid and the box of the interior end of sliding fit containing are formed, it is characterized in that: in box of the interior end, be provided with the reagent bottle that accounts for box length 1/2～3/5, the pore support, box box mouth appropriate position, the end in the frame plane of this support is located at and is lower than, and on this face, be provided with and arrange orderly big bottle, the pore hole, this support jam-pack is at an end of box of the interior end, is provided with packed plate and supplementary bottle in the space between the other end width facade of the other end stand wall of this support and box of the interior end, pipe; Be provided with on the plane, outer lid top in cover is buckled on the end box and arrange orderly bottle, pore hole, be covered with box on this hole and paste film.

Described bottle, pore support, the appropriate location at its place, plane is in 3/5～4/5 high position of box of the interior end, and big bottle, pore hole designed on this face are respectively: 1. two macroscopic voids of vacuolar membrane liquid and lapping liquid scintillation vial; 2. prehybridization mother liquor is gone up hole in three of cleaning fluid I and dilution buffer liquid B serum bottle; 3. two sealers, cleaning fluid II, three buffer A, hole down in nine of two dilution buffer liquid A and buffer B centrifuge tube; 4. digoxigenin-probe, two antibody, developer A, developer B, seven small holes of positive control and the little centrifuge tube of negative control.

Described outer lid, the bottle that is provided with on the Qi Ding plane, pore hole are: two rows or three rows arrange in order and cover the stealthy hole that box pastes film.

End box in described also is equipped with packed hybond membrane during its box is interior, hybridization bag, point sample kapillary and operation gloves.

The utility model has the advantages that because with detectable-digoxigenin-probe, antibody and various clean-out system sealer, buffering agent integrates box, and by detecting step process, organic being arranged on the support of hole, make and this detecting operation process simple possible be difficult for mistake, working specification, testing result is accurate, fast, sensitivity, safety.

Embodiment accompanying drawings of the present utility model is as follows:

Fig. 1 is the structure cut-open view (the A-A cut-open view of Fig. 2) of prawn cause of disease detection kit;

Fig. 2 is the hole shelf structure vertical view in the box of the interior end of prawn cause of disease detection kit;

Fig. 3 is the hole shelf structure upward view in the outer lid of prawn cause of disease detection kit.

Referring to Fig. 1-3, make prawn cause of disease detection kit made of paper, its physical dimension: 250 * 80 * 60mm, outer lid 1 sliding fit of its cuboid are included on the box of the interior end 2.In box of the interior end 2, be provided with reagent bottle, pore support 3, this support 3 size: 140 * 79 * 40mm.The box mouth place of end box 2 in the frame plane of this support 3 is located at and is lower than, and on this face, be provided with arrangement orderly big bottle, pore hole 6～21.These support 3 jam-packs are at an end of box of the interior end 2, are provided with packed plate 4 and supplementary bottle, pipe 5 in the space between the other end width facade of the other end stand wall of this support 3 and box of the interior end 2.Be provided with on the plane, outer lid 1 top in cover is buckled on the end box 2 and arrange orderly bottle, pore hole 22, be covered with box on this hole 22 and paste film (not drawing among the figure).

2/3 position (40mm) of end box 2 height (60mm) in reagent bottle, pore support 3 that this kit is designed, its plane are in.Big bottle, pore hole designed on this face are respectively: macroscopic void 6 and the macroscopic void 7 that the lapping compound scintillation vial (20ml volume) of 12ml is housed that the vacuolar membrane liquid scintillation vial (20ml volume) of 10ml 1. is housed.2. be equipped with 4ml prehybridization mother liquor serum bottle (4ml volume) in go up hole 8.Be equipped with 4ml cleaning fluid I serum bottle (4ml volume) in go up hole 9.Be equipped with 3ml dilution buffer liquid B serum bottle (4ml volume) in go up hole 10.3. two centrifuge tubes (hole 11 under in two of 1.5ml (volume) of the sealer of 40mg is housed respectively.Be equipped with 1.5ml cleaning fluid II centrifuge tube (1.5ml volume) in following hole 12.Hole 13 down is housed respectively among three of three centrifuge tubes (1.5ml volume) of buffer A of 1ml.Hole 14 down is housed among two of two centrifuge tubes (1.5ml volume) of dilution buffer liquid A of 1.5ml.Be equipped with 1ml buffer B centrifuge tube (1.5ml volume) in following hole 15.The small holes 16 of the little centrifuge tube of digoxigenin-probe (0.2ml volume) of 40 μ 1 4. is housed.Two small holes 17 of two little centrifuge tubes (0.2ml volume) of the antibody of 40 μ l are housed respectively.The small holes 18 of the little centrifuge tube of developer A (0.2ml volume) of 13.5 μ l is housed.The small holes 19 of the little centrifuge tube of developer B (0.2ml volume) of 10.5 μ l is housed.The small holes 20 of the little centrifuge tube of positive control (0.2ml volume) of 20 μ l is housed.The small holes 21 of the little centrifuge tube of negative control (0.2ml volume) of 20 μ l is housed.

The outer lid 1 of this reagent place design, the bottle that is provided with on the Qi Ding plane, pore hole are that two rows arrange the stealthy hole 22 that the covering box pastes film (not drawing among the figure) in order, during in order to practical operation, use as the reagent pipe support.

The box of the interior end 2 that this kit is designed also is equipped with packed hybond membrane 23 and hybridization bag 24 in its box 2, and point sample kapillary and operation gloves (not drawing among the figure).

Claims

1, a kind of prawn cause of disease detection kit, it is that the outer lid of cuboid and the box of the interior end of sliding fit containing are formed, it is characterized in that: in box of the interior end, be provided with the reagent bottle, the pore support that account for box length 1/2～3/5, box box mouth appropriate position, the end in the frame plane of this support is located at and is lower than, and on this face, be provided with and arrange orderly big bottle, pore hole, this support jam-pack is at an end of box of the interior end, is provided with packed plate and supplementary bottle, pipe in the space between the other end width facade of the other end stand wall of this support and box of the interior end; Be provided with on the plane, outer lid top in cover is buckled on the end box and arrange orderly bottle, pore hole, be covered with box on this hole and paste film.

2, prawn cause of disease detection kit according to claim 1, it is characterized in that: described bottle, pore support, the appropriate location at place, its plane is in 3/5～4/5 high position of box of the interior end, and big bottle, pore hole designed on this face are respectively: 1. two macroscopic voids of vacuolar membrane liquid and lapping liquid scintillation vial; 2. prehybridization mother liquor is gone up hole in three of cleaning fluid I and dilution buffer liquid B serum bottle; 3. two sealers, cleaning fluid II, three buffer A, hole down in nine of two dilution buffer liquid A and buffer B centrifuge tube; 4. digoxigenin-probe, two antibody, developer A, developer B, seven small holes of positive control and the little centrifuge tube of negative control.

3, prawn cause of disease detection kit according to claim 1 is characterized in that: described outer lid, the bottle that is provided with on the Qi Ding plane, pore hole are: two rows or three rows arrange in order and cover the stealthy hole that box pastes film.

4, prawn cause of disease detection kit according to claim 1 is characterized in that: end box in described, also be equipped with packed hybond membrane during its box is interior, hybridization bag, point sample kapillary and operation gloves.