CN215375439U - Three-item joint inspection rapid detection kit for clenbuterol - Google Patents
Three-item joint inspection rapid detection kit for clenbuterol Download PDFInfo
- Publication number
- CN215375439U CN215375439U CN202120860637.3U CN202120860637U CN215375439U CN 215375439 U CN215375439 U CN 215375439U CN 202120860637 U CN202120860637 U CN 202120860637U CN 215375439 U CN215375439 U CN 215375439U
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- CN
- China
- Prior art keywords
- detection
- clenbuterol
- kit
- tube
- syringe
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000001514 detection method Methods 0.000 title claims abstract description 32
- 229960001117 clenbuterol Drugs 0.000 title claims abstract description 10
- STJMRWALKKWQGH-UHFFFAOYSA-N clenbuterol Chemical compound CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 STJMRWALKKWQGH-UHFFFAOYSA-N 0.000 title claims abstract description 10
- 238000007689 inspection Methods 0.000 title abstract description 5
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 239000003480 eluent Substances 0.000 claims abstract description 9
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 6
- 239000002984 plastic foam Substances 0.000 claims abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 239000010902 straw Substances 0.000 claims description 2
- 229960001399 clenbuterol hydrochloride Drugs 0.000 abstract description 14
- OPXKTCUYRHXSBK-UHFFFAOYSA-N clenbuterol hydrochloride Chemical compound Cl.CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 OPXKTCUYRHXSBK-UHFFFAOYSA-N 0.000 abstract description 14
- NDAUXUAQIAJITI-UHFFFAOYSA-N albuterol Chemical compound CC(C)(C)NCC(O)C1=CC=C(O)C(CO)=C1 NDAUXUAQIAJITI-UHFFFAOYSA-N 0.000 abstract description 11
- YJQZYXCXBBCEAQ-UHFFFAOYSA-N ractopamine Chemical compound C=1C=C(O)C=CC=1C(O)CNC(C)CCC1=CC=C(O)C=C1 YJQZYXCXBBCEAQ-UHFFFAOYSA-N 0.000 abstract description 11
- 229940074095 ractopamine Drugs 0.000 abstract description 11
- 229960002052 salbutamol Drugs 0.000 abstract description 11
- 235000013622 meat product Nutrition 0.000 abstract description 4
- 238000012216 screening Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a clenbuterol three-item joint inspection rapid detection kit, which comprises a kit body, two bottles containing an extracting agent, a bottle containing eluent, a solid-phase extraction column, a 1.5ml centrifugal tube, a 50ml centrifugal tube, three detection cards, a 1ml suction tube and a 10ml syringe, wherein the two bottles containing the extracting agent and the bottle containing the eluent are sequentially placed on a plastic foam support with holes and grooves in the kit body; the 1.5ml centrifuge tube, the 50ml centrifuge tube, the solid phase extraction column, the 1ml suction tube and the 10ml syringe are all stored in the sealing bag, and the sealing bag is positioned in the box body. The kit can rapidly detect the residual quantity of clenbuterol hydrochloride (CLEN), Salbutamol (SAL) and Ractopamine (RAC) in meat products, and meets the detection requirement of on-site rapid screening.
Description
Technical Field
The utility model relates to the technical field of clenbuterol hydrochloride detection devices, in particular to a three-item joint inspection rapid detection kit for clenbuterol hydrochloride.
Background
At present, the analytical means for clenbuterol at home and abroad comprises a liquid/gas chromatography, a chromatography and an immunoassay. The liquid/gas combined method and the chromatography need large and expensive instruments, have high requirements on the quality of detection personnel, and cannot carry out field detection; immunoassay methods also suffer from the disadvantage of requiring expensive instrumentation and can only be used to detect single or structurally similar compounds.
Therefore, a detection means with low cost, low requirement on quality of detection personnel, field detection and good stability needs to be developed.
Disclosure of Invention
The utility model aims to provide a clenbuterol three-item joint inspection rapid detection kit, which can rapidly detect the residual quantity of clenbuterol hydrochloride (CLEN), Salbutamol (SAL) and Ractopamine (RAC) in meat products and meet the detection requirement of on-site rapid screening.
The purpose of the utility model is realized by the following technical scheme:
the utility model provides a three items of close quick detect reagent boxes of clenbuterol, the kit includes box body, two bottles that hold the extractant, one holds bottle, solid-phase extraction post, 1.5ml centrifuging tube, 50ml centrifuging tube, three detection card, 1ml straw, 10ml syringe of eluent, wherein:
two bottles containing extracting agents and one bottle containing eluent are sequentially placed on a plastic foam support with holes and grooves in the box body;
the 1.5ml centrifuge tube, the 50ml centrifuge tube, the solid phase extraction column, the 1ml suction tube and the 10ml syringe are all stored in the sealing bag, and the sealing bag is positioned in the box body.
The three detection cards comprise a sample adding hole and an observation window.
According to the technical scheme provided by the utility model, the kit can be used for rapidly detecting the residual quantity of clenbuterol hydrochloride (CLEN), Salbutamol (SAL) and Ractopamine (RAC) in meat products, and meets the detection requirement of on-site rapid screening.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a three-item kit for rapid detection of clenbuterol according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a detection card according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
The following embodiments of the present invention will be further described in detail with reference to the accompanying drawings, and as shown in fig. 1, it is a schematic structural diagram of three general-purpose kit for detecting clenbuterol provided by the embodiments of the present invention, the kit includes a box 1, two bottles (e.g., bottle 2 and bottle 3 in fig. 1) containing an extracting agent, one bottle 4 containing an eluent, a solid-phase extraction column 5, a 1.5ml centrifuge tube 6, a 50ml centrifuge tube 7, three detection cards 8, a 1ml pipette 9, and a 10ml syringe 10, wherein:
two bottles containing extracting agents and a bottle 4 containing eluent are sequentially placed on a plastic foam support 11 with holes and grooves in the box body 1;
the 1.5ml centrifuge tube 6, the 50ml centrifuge tube 7, the solid phase extraction column 5, the 1ml suction tube 9 and the 10ml syringe 10 are all stored in the sealing bag, and the sealing bag is positioned in the box body 1.
As shown in fig. 2, which is a schematic structural diagram of the detection card according to the embodiment of the present invention, the three detection cards 8 include a sample adding hole 81 and a viewing window 82. The letter C outside the viewing window 82 represents a control line and the letter T represents a test line.
The kit provided by the utility model is used for determining whether a sample contains clenbuterol hydrochloride (CLEN), Salbutamol (SAL) and Ractopamine (RAC) by adopting an immune competition method analysis principle and combining a colloidal gold labeling technology, and the specific test process is as follows:
firstly, weighing 10g of tissue and muscle sample after crushing or homogenizing, placing the tissue and muscle sample into a 50mL centrifuge tube 7, adding 5mL of extractant 1, shaking for 2min, adding 10mL of extractant 2, shaking vigorously for 5mn, centrifuging for 5min at 4000r/min, and taking upper-layer liquid as sample treatment liquid.
Then, the 10mL syringe 10 was connected and fixed to the solid phase extraction column using a red adapter, and then connected to the SPE device. And (3) sucking the supernatant of the treatment solution into a syringe 10, starting an SPE vacuum pump, controlling a valve to enable the liquid to flow through the solid-phase extraction column 5 at the uniform speed of 2-3d/S, taking down a syringe 10 cylinder, continuing for 10S, twisting an exhaust valve until the pointer returns to 0, and then closing the vacuum pump. The 1.5mL centrifuge tube 6 was placed in the corresponding test solution collection rack and placed in the waste solution collection jar. Place the upper cover plate on the test solution collection jar, ensure to align the pipeline below the upper cover plate with centrifuge tube 6. And adding 0.4mL of eluent into the solid-phase extraction column 5, then starting an SPE vacuum pump, controlling a valve to enable the liquid to uniformly flow through the solid-phase extraction column 5 at a speed of 1-2d/s, after all the eluent flows through the solid-phase extraction column 5, continuing for 10s, closing the vacuum pump, and uniformly mixing the liquid in the centrifuge tube 6 to obtain the detection liquid.
Then, 3 CLEN (CLEN, RAC, SAL) detection cards 8 are respectively taken out from the original packaging bags, numbered and marked, horizontally placed on the front side of an observer, a suction pipe 9 attached to the sealing bag is used for sucking detection liquid, 4-5 drops (about 110uL) are respectively dropped into sample adding holes 81 of the three detection cards 8, and the result is read (about 8-10 min) after the colors of the color development areas of the three detection cards 8 are clear and stable.
It is noted that those skilled in the art will recognize that embodiments of the present invention are not described in detail herein.
In conclusion, the kit has the shockproof function, and can avoid the breakage of glass bottles; the sealing is good, and the external pollution is avoided; the detection steps are simple and quick, the error is small, and the kit can be used for quickly screening clenbuterol hydrochloride (CLEN), Salbutamol (SAL) and Ractopamine (RAC) in meat products on site.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (2)
1. The utility model provides a three items of clenbuterol close quick detect reagent box, a serial communication port, the kit includes box body, two bottles that hold the extractant, one holds bottle, solid-phase extraction post, 1.5ml centrifuging tube, 50ml centrifuging tube, three detection cards, 1ml straw, 10ml syringe that hold the eluant, wherein:
two bottles containing extracting agents and one bottle containing eluent are sequentially placed on a plastic foam support with holes and grooves in the box body;
the 1.5ml centrifuge tube, the 50ml centrifuge tube, the solid phase extraction column, the 1ml suction tube and the 10ml syringe are all stored in the sealing bag, and the sealing bag is positioned in the box body.
2. The kit for the three-item combined detection and rapid detection of clenbuterol according to claim 1,
the three detection cards comprise a sample adding hole and an observation window.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120860637.3U CN215375439U (en) | 2021-04-25 | 2021-04-25 | Three-item joint inspection rapid detection kit for clenbuterol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120860637.3U CN215375439U (en) | 2021-04-25 | 2021-04-25 | Three-item joint inspection rapid detection kit for clenbuterol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215375439U true CN215375439U (en) | 2021-12-31 |
Family
ID=79627981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120860637.3U Expired - Fee Related CN215375439U (en) | 2021-04-25 | 2021-04-25 | Three-item joint inspection rapid detection kit for clenbuterol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215375439U (en) |
-
2021
- 2021-04-25 CN CN202120860637.3U patent/CN215375439U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220309 Address after: 100081 No. 12 South Main Street, Haidian District, Beijing, Zhongguancun Patentee after: INSTITUTE OF QUALITY STANDARD AND TESTING TECHNOLOGY FOR AGRO-PRODUCTS, CHINESE ACADAMY OF AGRICULTURAL SCIENCES Patentee after: BEIJING LIUJIAOTI TECHNOLOGY DEVELOPMENT CO.,LTD. Address before: 100081 No. 12 South Main Street, Haidian District, Beijing, Zhongguancun Patentee before: INSTITUTE OF QUALITY STANDARD AND TESTING TECHNOLOGY FOR AGRO-PRODUCTS, CHINESE ACADAMY OF AGRICULTURAL SCIENCES Patentee before: Beijing hexagon Testing Technology Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211231 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |