CN102262082B - Optical fiber sensor-molecular imprinting online detection method for detecting propofol in biological samples and optical fiber sensor-molecular imprinting online detection device. - Google Patents
Optical fiber sensor-molecular imprinting online detection method for detecting propofol in biological samples and optical fiber sensor-molecular imprinting online detection device. Download PDFInfo
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Abstract
An optical fiber sensor-molecular imprinting online detection method for detecting propofol in biological samples and an optical fiber sensor-molecular imprinting online detection device. The device provided by the invention comprises solution assay flasks, connection standpipes, a suction pump, a horizontal pipe, a liquid storage pipe, a switch valve, a fiber optically focused instrument, a right connecting pipe and a left connecting pipe. The device provided by the invention has a reasonable and compact structure; the method provided by the invention is simple and is easy to operate; according to the invention, continuous real-time online long-distance monitoring can be realized so as to enable the detection of propofol in the biological samples to be simpler, more time-saving and accurate; the interference of complex components in the biological samples is minimized and the detection efficiency is also greatly raised, thus completing the pretreatment and detection of the propofol-containing biological samples within minutes; as the optical signal transmission of propofol is completed by the utilization of optical fiber chemical sensing, the fine property and bendability of the optical fiber enable to simplify the rigid structure of instruments and equipment and continuously complete the pretreatment and detection of the samples at a time, thus avoiding the decentralization and incoherence of sampling analysis as well as result errors in present methods.
Description
Technical field
The present invention relates to the technical field that in biosome, propofol detects, is method and the optical fiber sensor-molecular imprinting on-line measuring device of propofol in the online detection of biological sample of a kind of optical fiber sensor-molecular imprinting.
Background technology
Propofol (Propofol) 2,6-diisopropyl phenol, it is a kind of intravenous anesthetics, found in 1970, clinical testing first in 1977, within 1989, enter clinical practice through FDA approval, because of the characteristic such as rapid-action, action time is short, revive rapidly, be it is called the intravenous arcotic into " cross-centennial ".At present, be widely used in clinical anesthesia and ICU calmness both at home and abroad.
At present, the biology sample detection of propofol adopts HPLC method more, gas phase-mass spectrography, and fluorescent spectrometries etc. detect its concentration, and these method majorities are sample analysis, and collected specimens, sample pre-treatments and sample analysis detect all and carry out step by step.Sample analysis can only provide the drug concentration information of time point, data bulk is limited, disperses discontinuously, easily causes information dropout or skew, particularly, for both can complete the ultrashort effect medicine of whole metabolic process at tens minutes, can not reflect the situation of the whole metabolic process of medicine.Sampling testing process complicated and time consumption, and in sample storage process, constituent can change, and causes resultant error.
Because mostly propofol biological sample is blood sample, complicated component in blood, contains multiple proteins and blood cell, as deals with accuracy and the validity that can directly affect sample determination improperly, therefore biological sample pre-treatment is the bottleneck of analyzing and testing, is the committed step in analytical approach.Propofol biological sample pre-treatment at present adopts precipitation of protein and liquid-liquid extraction method more.The precipitation method can make propofol cause the recovery low with albumen co-precipitation, and resultant error is large.When liquid-liquid extraction method is used for extracting blood plasma propofol, linear relationship between extracted amount and propofol is undesirable, in solvent evaporates process, and there is the possibility of propofol with the volatilization of nitrogen stream, the method complicated and time consumption, organic solvent consumption is large, normal emulsion and the tested component loss etc. of occurring in leaching process.
Because propofol in vivo accretion rate is fast, make it must continuous infusion, and accretion rate be different because of individual difference, therefore thirsts for the blood concentration of continuous detecting propofol.And above-mentioned prior art is difficult to realize continuous detecting.
Summary of the invention
The invention provides method and the optical fiber sensor-molecular imprinting on-line measuring device of propofol in the online detection of biological sample of a kind of optical fiber sensor-molecular imprinting, overcome the deficiency of above-mentioned prior art, it can effectively solve sampling testing process complicated and time consumption, and in sample storage process, constituent can change, and causes the problem of resultant error.
One of technical scheme of the present invention realizes by following measures: a kind of optical fiber sensor-molecular imprinting on-line measuring device, comprises solution trial jar, connects standpipe, suction pump, horizontal tube, liquid storage pipe, switch valve, optical fiber optically focused instrument, right connecting pipe and left connecting pipe, the upper end that is no less than the solution trial jar of three is installed with respectively connection standpipe, be installed with the horizontal tube communicating with the inlet of suction pump at the liquid feeding end of suction pump, the upper end that connects standpipe links together with horizontal tube respectively and is connected, on connection standpipe, be installed with valve, outlet end in suction pump is installed with liquid storage pipe, lower end at liquid storage pipe is installed with switch valve, the mounting hole that has left and right directions to communicate on optical fiber optically focused instrument, in mounting hole, be installed with quartz ampoule, the right-hand member of quartz ampoule links together by right connecting pipe and switch valve, the left end of quartz ampoule is through mounting hole and left connecting pipe is installed, there are respectively the light well and the light hole that communicate with mounting hole in top and front side at optical fiber optically focused instrument.
The further optimization and/or improvements to one of foregoing invention technical scheme below:
Above-mentioned solution trial jar can comprise methyl alcohol trial jar, distilled water trial jar and plasma sample trial jar; Methyl alcohol trial jar, distilled water trial jar and plasma sample trial jar are spaced, and between the upper end of the connection standpipe of distilled water trial jar and plasma sample trial jar and horizontal tube, are installed with three-way change-over valve.
The outer end of above-mentioned left connecting pipe can be connected with the waste collection bottle being connected with left connecting pipe.
On light well on above-mentioned optical fiber optically focused instrument and light hole, can be installed with respectively Connection Block; Connection Block comprises pedestal, positioning screw, outer cylinder body and inner barrel; In pedestal, there is the through hole communicating with inner barrel and outer cylinder body inner chamber, the inner of pedestal and the outer end of inner barrel are fixed together, the inner side, outer end of pedestal is fixedly installed togather by screw thread with the inner outside of inner barrel, the outside of inner barrel is fixedly mounted on respectively in light well and light hole by screw thread, in inner barrel, be installed with optical fiber optically focused probe, on pedestal and outer cylinder body, have the radial hole of at least one, in radial hole, being provided with by screw thread can inside and outside mobile positioning screw.
In above-mentioned outer cylinder body, can be installed with the optical fiber of the screw rod location that can be positioned.
Above-mentioned liquid storage pipe can be connected and be communicated by upper connecting pipe with the outlet end of suction pump, and the lower end of liquid storage pipe can be connected and be communicated by lower connecting pipe with switch valve, and upper connecting pipe and lower connecting pipe are flexible pipe.
Two of technical scheme of the present invention realizes by following measures: a kind of method of propofol in online detection of biological sample of optical fiber sensor-molecular imprinting that utilizes above-mentioned optical fiber sensor-molecular imprinting on-line measuring device, it carries out in the steps below: first plasma sample, distilled water and methyl alcohol are respectively charged in plasma sample trial jar, distilled water trial jar and methyl alcohol trial jar, when work, in liquid storage pipe, load the propofol molecularly imprinted polymer of set amount; Open the three-way change-over valve of controlling blood sample, suction pump is opened, by suction pump, plasma sample is entered in liquid storage pipe, now switch valve is in closed condition; When the height of specimen in liquid storage pipe is during to set amount, close suction pump, plasma sample is no longer flowed in liquid storage pipe; Jolting liquid storage pipe makes polymkeric substance fully contact with plasma sample for two minutes, and now the propofol in biological sample can be combined with imprinted polymer by intermolecular force, and propofol molecule is embedded in reserved hole, completes " seizure " process; The three-way change-over valve of closing control plasma sample trial jar afterwards, open the three-way change-over valve of controlling distilled water trial jar, suction pump and switch valve are opened, distilled water is taken away comprise blood plasma and protein in biological sample simultaneously, and propofol molecule can be stayed in hole with polymkeric substance strong bonded; Then close the three-way change-over valve of distilled water trial jar and open the three-way change-over valve of methyl alcohol trial jar, make methyl alcohol flow into liquid storage pipe, during highly to set amount, close suction pump, jolting liquid storage pipe, propofol is soluble in methyl alcohol, by jolting, propofol is eluted and is dissolved in methyl alcohol from hole, and now in methyl alcohol, the amount of propofol is the content of propofol in biological sample.
Three of technical scheme of the present invention realizes by following measures: a kind of method of propofol in online detection of biological sample of optical fiber sensor-molecular imprinting that utilizes above-mentioned optical fiber sensor-molecular imprinting on-line measuring device, it carries out in the steps below: first plasma sample, distilled water and methyl alcohol are respectively charged in plasma sample trial jar, distilled water trial jar and methyl alcohol trial jar, when work, in liquid storage pipe, load about 20mg propofol molecularly imprinted polymer; Open the three-way change-over valve of controlling blood sample, suction pump is opened, by suction pump, plasma sample is entered in liquid storage pipe, now switch valve is in closed condition; When the height of specimen in liquid storage pipe is during to 2mL, close suction pump, plasma sample is no longer flowed in liquid storage pipe; Jolting liquid storage pipe makes polymkeric substance fully contact with plasma sample for two minutes, and now the propofol in biological sample can be combined with imprinted polymer by intermolecular force, and propofol molecule is embedded in reserved hole, completes " seizure " process; The three-way change-over valve of closing control plasma sample trial jar afterwards, open the three-way change-over valve of controlling distilled water trial jar, suction pump and switch valve are opened simultaneously, distilled water is pulled away comprise blood plasma, protein etc. in biological sample with the flow velocity of 6mL/min, and propofol molecule can be stayed in hole with polymkeric substance strong bonded; After 1 minute, close the three-way change-over valve of distilled water trial jar and open the three-way change-over valve of methyl alcohol trial jar, make methyl alcohol flow into liquid storage pipe, during highly to 2mL, close suction pump, jolting liquid storage pipe, propofol is soluble in methyl alcohol, by jolting, propofol is eluted and is dissolved in 2mL methyl alcohol from hole, and now in methyl alcohol, the amount of propofol is the content of propofol in biological sample; Switch valve is opened, and this methanol solution flows in the quartz ampoule of optical fiber optically focused instrument; Itself has fluorescent characteristic propofol, light source produces by concentration piece and optical filter the light that wavelength is 279nm, utilize optical fiber 23 and optical fiber optically focused probe to light shine on quartz ampoule, propofol in sample is subject to exciting of 279nm light and launches the light signal of 300nm, again by optical fiber by light signal input detector and data handling system, obtain fluorescent value, by propofol in the known sample of regression equation, so just completed the detection to propofol in biological sample.
Reasonable and compact in structure of the present invention, method are simple to operation, because propofol has the character of emitting fluorescence, utilize fiber optical chemistry sensor to complete the conduction of photosignal, thus can realize continuously, in real time, online, remote monitoring make more easy to the detection of propofol in biological sample, save time, accurately; This has not only reduced the interference of complicated ingredient in biological sample but also has greatly improved detection efficiency, makes the pre-treatment of propofol biological sample and detect can complete in several minutes; Utilize fiber optical chemistry sensor to complete the optical signal transmission of propofol, fine and the bending property of optical fiber has not only been simplified the rigid structure of apparatus, and make sample pre-treatments and detect continuously disposable completing, avoid the discontinuous and resultant error of the dispersion of sample analysis in existing method.
Brief description of the drawings
Accompanying drawing 1 is the best main TV structure schematic diagram of implementing of the present invention.
The A-A that accompanying drawing 2 is accompanying drawing 1 is to analysing and observe structure for amplifying schematic diagram.
Coding in accompanying drawing is respectively: 1 for connecting standpipe, 2 is suction pump, 3 is horizontal tube, 4 is liquid storage pipe, 5 is switch valve, 6 is optical fiber optically focused instrument, 7 is quartz ampoule, 8 is right connecting pipe, 9 is left connecting pipe, 10 is light well, 11 is light hole, 12 is methyl alcohol trial jar, 13 is distilled water trial jar, 14 is plasma sample trial jar, 15 is three-way change-over valve, 16 is waste collection bottle, 17 is pedestal, 18 is positioning screw, 19 is outer cylinder body, 20 is inner barrel, 21 is optical fiber optically focused probe, 22 is upper connecting pipe, 22 is optical fiber, 23 is upper connecting pipe, 24 is lower connecting pipe.
Embodiment
The present invention is not subject to the restriction of following embodiment, can determine concrete embodiment according to technical scheme of the present invention and actual conditions.
In the present invention, for convenience of description, the description of the relative position relation of each parts is to be all described according to the Butut mode of Figure of description, as: the position relationship of upper and lower, left and right etc. is to determine according to the Butut direction of Figure of description.
Below in conjunction with embodiment and accompanying drawing, the invention will be further described:
Embodiment 1, as shown in accompanying drawing 1,2, this optical fiber sensor-molecular imprinting on-line measuring device comprises solution trial jar, connects standpipe 1, suction pump 2, horizontal tube 3, liquid storage pipe 4, switch valve 5, optical fiber optically focused instrument 6, right connecting pipe 8 and left connecting pipe 9, the upper end that is no less than the solution trial jar of three is installed with respectively and connects standpipe 1, be installed with the horizontal tube 3 communicating with the inlet of suction pump 2 at the liquid feeding end of suction pump 2, the upper end that connects standpipe 1 links together and is connected with horizontal tube 3 respectively, on connection standpipe 1, be installed with valve, be installed with liquid storage pipe 4 at the outlet end of suction pump 2, be installed with switch valve 5 in the lower end of liquid storage pipe 4, the mounting hole that has left and right directions to communicate on optical fiber optically focused instrument 6, in mounting hole, be installed with quartz ampoule 7, the right-hand member of quartz ampoule 7 links together with switch valve 5 by right connecting pipe 8, the left end of quartz ampoule 7 is through mounting hole and left connecting pipe 9 is installed, there are respectively the light well 10 and the light hole 11 that communicate with mounting hole in top and front side at optical fiber optically focused instrument 6.
Can according to actual needs, above-mentioned optical fiber sensor-molecular imprinting on-line measuring device be made further optimization and/or improvements:
As shown in Figure 1, solution trial jar comprises methyl alcohol trial jar 12, distilled water trial jar 13 and plasma sample trial jar 14; Methyl alcohol trial jar 12, distilled water trial jar 13 and plasma sample trial jar 14 are spaced, and between the upper end of the connection standpipe 1 of distilled water trial jar 13 and plasma sample trial jar 14 and horizontal tube 3, are installed with three-way change-over valve 15.
As shown in Figure 1, the outer end of left connecting pipe 9 is connected with the waste collection bottle 16 being connected with left connecting pipe 9.
As shown in Figure 2, on the light well 10 on optical fiber optically focused instrument 6 and light hole 11, be installed with respectively Connection Block; Connection Block comprises pedestal 17, positioning screw 18, outer cylinder body 19 and inner barrel 20; In pedestal 17, there is the through hole communicating with inner barrel 20 and outer cylinder body 19 inner chambers, the outer end of the inner of pedestal 17 and inner barrel 20 is fixed together, the inner side, outer end of pedestal 17 is fixedly installed togather by screw thread with the inner outside of inner barrel 20, the outside of inner barrel 20 is fixedly mounted on respectively in light well 10 and light hole 11 by screw thread, in inner barrel 20, be installed with optical fiber optically focused probe 21, on pedestal 17 and outer cylinder body 19, have the radial hole of at least one, in radial hole, being provided with by screw thread can inside and outside mobile positioning screw 18.
As shown in Figure 2, in outer cylinder body 19, be installed with the optical fiber 22 that the screw rod 18 that can be positioned is located.
As shown in Figure 1, liquid storage pipe 4 is connected and is communicated by upper connecting pipe 23 with the outlet end of suction pump 2, and the lower end of liquid storage pipe 4 is connected and is communicated by lower connecting pipe 24 with switch valve 5, and upper connecting pipe 23 and lower connecting pipe 24 are flexible pipe; Can be by the potpourri in concussion liquid storage pipe 4, fast reaction speed.
Embodiment 2, this utilizes the method for propofol in the online detection of biological sample of optical fiber sensor-molecular imprinting of above-mentioned optical fiber sensor-molecular imprinting on-line measuring device to carry out in the steps below: first plasma sample, distilled water and methyl alcohol are respectively charged in plasma sample trial jar 14, distilled water trial jar 13 and methyl alcohol trial jar 12, when work, in liquid storage pipe 4, load the propofol molecularly imprinted polymer of set amount; Open the three-way change-over valve 15 of controlling blood sample, suction pump 2 is opened, by suction pump 2, plasma sample is entered in liquid storage pipe 4, now switch valve 5 is in closed condition; When the height of specimen in liquid storage pipe 4 is during to set amount, close suction pump 2, plasma sample is no longer flowed in liquid storage pipe 4; Jolting liquid storage pipe makes polymkeric substance fully contact with plasma sample for 4 two minutes, and now the propofol in biological sample can be combined with imprinted polymer by intermolecular force, and propofol molecule is embedded in reserved hole, completes " seizure " process; The three-way change-over valve 15 of closing control plasma sample trial jar 14 afterwards, open the three-way change-over valve 15 of controlling distilled water trial jar 13, suction pump 2 and switch valve 5 are opened simultaneously, distilled water is taken away comprise blood plasma and protein in biological sample, and propofol molecule can be stayed in hole with polymkeric substance strong bonded; Then close the three-way change-over valve 15 of distilled water trial jar 13 and open the three-way change-over valve 15 of methyl alcohol trial jar 12, make methyl alcohol flow into liquid storage pipe 4, during highly to set amount, close suction pump 2, jolting liquid storage pipe 4, propofol is soluble in methyl alcohol, by jolting, propofol is eluted and is dissolved in methyl alcohol from hole, and now in methyl alcohol, the amount of propofol is the content of propofol in biological sample.
Embodiment 3, this utilizes the method for propofol in the online detection of biological sample of optical fiber sensor-molecular imprinting of above-mentioned optical fiber sensor-molecular imprinting on-line measuring device to carry out in the steps below: first plasma sample, distilled water and methyl alcohol are respectively charged in plasma sample trial jar 14, distilled water trial jar 13 and methyl alcohol trial jar 12, when work, in liquid storage pipe 4, load about 20mg propofol molecularly imprinted polymer; Open the three-way change-over valve 15 of controlling blood sample, suction pump 2 is opened, by suction pump 2, plasma sample is entered in liquid storage pipe 4, now switch valve 5 is in closed condition; When the height of specimen in liquid storage pipe 4 is during to 2mL, close suction pump 2, plasma sample is no longer flowed in liquid storage pipe 4; Jolting liquid storage pipe makes polymkeric substance fully contact with plasma sample for 4 two minutes, and now the propofol in biological sample can be combined with imprinted polymer by intermolecular force, and propofol molecule is embedded in reserved hole, completes " seizure " process; The three-way change-over valve 15 of closing control plasma sample trial jar 14 afterwards, open the three-way change-over valve 15 of controlling distilled water trial jar 13, suction pump 2 and switch valve 5 are opened simultaneously, distilled water is taken away comprise blood plasma, protein in biological sample with the flow velocity of 6mL/min, and propofol molecule can be stayed in hole with polymkeric substance strong bonded; After 1 minute, close the three-way change-over valve 15 of distilled water trial jar 13 and open the three-way change-over valve 15 of methyl alcohol trial jar 12, make methyl alcohol flow into liquid storage pipe 4, during highly to 2mL, close suction pump 2, jolting liquid storage pipe 4, propofol is soluble in methyl alcohol, by jolting, propofol is eluted and is dissolved in 2mL methyl alcohol from hole, and now in methyl alcohol, the amount of propofol is the content of propofol in biological sample; Switch valve 5 is opened, and this methanol solution flows in the quartz ampoule 7 of optical fiber optically focused instrument 6; Itself has fluorescent characteristic propofol, light source produces by concentration piece and optical filter the light that wavelength is 279nm, utilize optical fiber 23 and optical fiber optically focused probe 21 to light shine on quartz ampoule 7, propofol in sample is subject to exciting of 279nm light and launches the light signal of 300nm, pass through again optical fiber 22 by light signal input detector and data handling system, obtain fluorescent value, by propofol in the known sample of regression equation, so just completed the detection to propofol in biological sample.
Above technical characterictic has formed most preferred embodiment of the present invention, and it has stronger adaptability and best implementation result, can increase and decrease according to actual needs non-essential technical characterictic, meets the demand of different situations.
Adopt the process of the test of above-described embodiment 3:
1.1 use embodiment 3 detect propofol in blood plasma, and this process is lasted and is about 5 minutes.
The preparation of 1.2 typical curves
Propofol titer is mixed with blood plasma, be mixed with concentration and be respectively 0.1,0.5, the plasma sample solution of 1.0,2.0,5.0,10.0,15.0 μ g/mL, and measure its fluorescence intensity according to the operating process in 2.1.Numerical value, in table 1, obtains regression equation C=1.3074 F-9.3762, R
2=0.9896.
The detection of 1.3 plasma samples
In fresh plasma, add respectively 0.5,2.5,5.0,10.0,25.0,50.0, the propofol injection emulsion of 75.0 μ g, it is 0.1,0.5 that jolting is mixed to get blood concentration, 1.0,2.0,5.0,10.0, the series of samples of 15.0 μ g/mL, from adding propofol to have 15min to detecting sample, can make the composition of propofol in blood plasma be combined and Reality simulation state like this.Test and record data according to the operating process of embodiment 3, result is as table 2.
1.4 recovery test
In fresh plasma, add the propofol injection emulsion of 25 μ g, making background sample concentration is 5 μ g/mL, adds respectively propofol standard items 1.0,5.0,25 μ g, and measures its fluorescence intensity calculate recovery rate according to the operating process in 2.1, the results are shown in Table 3.
2.1 use embodiment 3 detect propofol in whole blood, and this process is lasted and is about 5 minutes.
The preparation of 2.2 typical curves
Propofol titer is mixed with the fresh heparin sheep blood that adds, be mixed with concentration and be respectively 0.1,0.5,1.0, the blood sample solution of 2.0,5.0,10.0,15.0 μ g/mL, and according to its fluorescence intensity of step measurements in 2.1, numerical value, in table 4, obtains regression equation C=2.0643 F-31.945, R
2=0.9354.
The detection of 2.3 whole blood samples
In the fresh sheep blood that adds heparin, add respectively 0.5,2.5,5.0,10.0,25.0,50.0, the propofol injection emulsion of 75.0 μ g, it is 0.1,0.5 that jolting is mixed to get blood concentration, 1.0,2.0,5.0,10.0, the series of samples of 15.0 μ g/mL, from adding propofol to have 15min to detecting sample, can make the composition of propofol in blood be combined and Reality simulation state like this.Test and record data by the operating process of embodiment 3, result is as table 5.
2.4 recovery test
In the fresh sheep blood that adds heparin, add the propofol injection emulsion of 25 μ g, making background sample concentration is 5 μ g/mL, adds respectively propofol standard items 1.0,5.0,25 μ g, and measure its fluorescence intensity calculate recovery rate according to the operating process in 2.1, the results are shown in Table 6.
For drug disposition trace analysis, background interference is large, is difficult to ensure good linearity and the recovery.Because the inventive method is in the time measuring in blood plasma and whole blood the propofol small concentration group recovery, exceed system detection sensitivity, there is the phenomenon that recovery deviation is larger, and still have higher sensitivity and accuracy in middle and high concentration group.
3 with the comparative test result comparison of prior art
Propofol biological sample in above-mentioned process of the test (blood plasma, whole blood), by using the blood concentration of HPLC method analyzing and testing propofol after Solid-Phase Extraction, be the results are shown in Table to 7,8, and compare with method of the present invention (being FOCS).
The equal not statistically significant of propofol difference (P > 0.05) in blood plasma and the whole blood recording through two kinds of methods of paired t-test (inspection level: α=0.05).
Method of the present invention has realized pre-treatment continuous, in place and the analyzing and testing fast of propofol biological sample, and its result difference but contrasts not statistically significant (P>0.05) with HPLC method.
Claims (3)
1. an optical fiber sensor-molecular imprinting on-line measuring device, is characterized in that comprising solution trial jar, connects standpipe, suction pump, horizontal tube, liquid storage pipe, switch valve, optical fiber optically focused instrument, right connecting pipe and left connecting pipe, the upper end that is no less than the solution trial jar of three is installed with respectively connection standpipe, be installed with the horizontal tube communicating with the inlet of suction pump at the liquid feeding end of suction pump, the upper end that connects standpipe links together with horizontal tube respectively and is connected, outlet end in suction pump is installed with liquid storage pipe, lower end at liquid storage pipe is installed with switch valve, the mounting hole that has left and right directions to communicate on optical fiber optically focused instrument, in mounting hole, be installed with quartz ampoule, the right-hand member of quartz ampoule links together by right connecting pipe and switch valve, the left end of quartz ampoule is through mounting hole and left connecting pipe is installed, there are respectively the light well and the light hole that communicate with mounting hole in top and front side at optical fiber optically focused instrument, wherein:
Solution trial jar comprises methyl alcohol trial jar, distilled water trial jar and plasma sample trial jar; Methyl alcohol trial jar, distilled water trial jar and plasma sample trial jar are spaced, and between the upper end of the connection standpipe of distilled water trial jar and plasma sample trial jar and horizontal tube, are installed with three-way change-over valve;
The outer end of left connecting pipe is connected with the waste collection bottle being connected with left connecting pipe;
On light well on optical fiber optically focused instrument and light hole, be installed with respectively Connection Block; Connection Block comprises pedestal, positioning screw, outer cylinder body and inner barrel; In pedestal, there is the through hole communicating with inner barrel and outer cylinder body inner chamber, the inner of pedestal and the outer end of inner barrel are fixed together, the inner side, outer end of pedestal is fixedly installed togather by screw thread with the inner outside of outer cylinder body, the outside of inner barrel is fixedly mounted on respectively in light well and light hole by screw thread, in inner barrel, be installed with optical fiber optically focused probe, on pedestal and outer cylinder body, there is the radial hole of at least one, in radial hole, by screw thread, the inside and outside mobile positioning screw of energy is installed;
In outer cylinder body, be installed with the optical fiber of the screw rod location that can be positioned;
Liquid storage pipe is connected and is communicated by upper connecting pipe with the outlet end of suction pump, and the lower end of liquid storage pipe is connected and is communicated by lower connecting pipe with switch valve, and upper connecting pipe and lower connecting pipe are flexible pipe.
2. the method for propofol in the online detection of biological plasma sample of optical fiber sensor-molecular imprinting that utilizes the optical fiber sensor-molecular imprinting on-line measuring device described in claim 1, it is characterized in that carrying out in the steps below: first biological plasma sample, distilled water and methyl alcohol are respectively charged in plasma sample trial jar, distilled water trial jar and methyl alcohol trial jar, when work, in liquid storage pipe, load the propofol molecularly imprinted polymer of set amount; Open the three-way change-over valve of controlling plasma sample trial jar, suction pump is opened, by suction pump, biological plasma sample is entered in liquid storage pipe, now switch valve is in closed condition; When the biological plasma sample height in liquid storage pipe is during to set amount, close suction pump, biological plasma sample is no longer flowed in liquid storage pipe; Jolting liquid storage pipe makes imprinted polymer fully contact with biological plasma sample for two minutes, and now the propofol in biological plasma sample can be combined with imprinted polymer by intermolecular force, and propofol molecule is embedded in reserved hole, completes " seizure " process; The three-way change-over valve of closing control plasma sample trial jar afterwards, open the three-way change-over valve of controlling distilled water trial jar, suction pump and switch valve are opened simultaneously, distilled water is taken away the blood plasma in biological plasma sample and protein, and propofol molecule can be stayed in hole with imprinted polymer strong bonded; Then close the three-way change-over valve of distilled water trial jar and open the three-way change-over valve of methyl alcohol trial jar, make methyl alcohol flow into liquid storage pipe, during highly to set amount, close suction pump, jolting liquid storage pipe, propofol is soluble in methyl alcohol, by jolting, propofol is eluted and is dissolved in methyl alcohol from hole, and now in methyl alcohol, the amount of propofol is the content of propofol in biological plasma sample.
3. the method for propofol in the online detection of biological plasma sample of optical fiber sensor-molecular imprinting that utilizes the optical fiber sensor-molecular imprinting on-line measuring device described in claim 1, it is characterized in that carrying out in the steps below: first biological plasma sample, distilled water and methyl alcohol are respectively charged in plasma sample trial jar, distilled water trial jar and methyl alcohol trial jar, when work, in liquid storage pipe, load about 20mg propofol molecularly imprinted polymer; Open the three-way change-over valve of controlling plasma sample trial jar, suction pump is opened, by suction pump, biological plasma sample is entered in liquid storage pipe, now switch valve is in closed condition; When the biological plasma sample height in liquid storage pipe is during to 2mL, close suction pump, biological plasma sample is no longer flowed in liquid storage pipe; Jolting liquid storage pipe makes imprinted polymer fully contact with biological plasma sample for two minutes, and now the propofol in biological plasma sample can be combined with imprinted polymer by intermolecular force, and propofol molecule is embedded in reserved hole, completes " seizure " process; The three-way change-over valve of closing control plasma sample trial jar afterwards, open the three-way change-over valve of controlling distilled water trial jar, suction pump and switch valve are opened simultaneously, distilled water is taken away the blood plasma in biological plasma sample, protein with the flow velocity of 6mL/min, and propofol molecule can be stayed in hole with imprinted polymer strong bonded; After 1 minute, close the three-way change-over valve of distilled water trial jar and open the three-way change-over valve of methyl alcohol trial jar, make methyl alcohol flow into liquid storage pipe, during highly to 2mL, close suction pump, jolting liquid storage pipe, propofol is soluble in methyl alcohol, by jolting, propofol is eluted and is dissolved in 2mL methyl alcohol from hole, and now in methyl alcohol, the amount of propofol is the content of propofol in biological plasma sample; Switch valve is opened, and this methanol solution flows in the quartz ampoule of optical fiber optically focused instrument; Itself has fluorescent characteristic propofol, light source produces by concentration piece and optical filter the light that wavelength is 279nm, utilize optical fiber and optical fiber optically focused probe to light shine on quartz ampoule, propofol in biological plasma sample is subject to exciting of 279nm light and launches the light signal of 300nm, again by optical fiber by light signal input detector and data handling system, obtain fluorescent value, by propofol in the known biological plasma sample of regression equation, so just complete the detection to propofol in biological plasma sample.
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|---|---|---|---|
| CN201110193659.XA CN102262082B (en) | 2011-07-12 | 2011-07-12 | Optical fiber sensor-molecular imprinting online detection method for detecting propofol in biological samples and optical fiber sensor-molecular imprinting online detection device. |
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| CN201110193659.XA CN102262082B (en) | 2011-07-12 | 2011-07-12 | Optical fiber sensor-molecular imprinting online detection method for detecting propofol in biological samples and optical fiber sensor-molecular imprinting online detection device. |
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| CN102262082A CN102262082A (en) | 2011-11-30 |
| CN102262082B true CN102262082B (en) | 2014-07-09 |
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| CN201110193659.XA Expired - Fee Related CN102262082B (en) | 2011-07-12 | 2011-07-12 | Optical fiber sensor-molecular imprinting online detection method for detecting propofol in biological samples and optical fiber sensor-molecular imprinting online detection device. |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2017133794A1 (en) * | 2016-02-02 | 2017-08-10 | Fresenius Kabi Deutschland Gmbh | Method and device for muasuring a concentration of at least one drug in an exhalation air of a patient |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104977282B (en) * | 2015-07-02 | 2017-06-30 | 山东省科学院海洋仪器仪表研究所 | The device and method of selenium in molecular engram selection laser inductive fluorescence method measurement water body |
| US20230293063A1 (en) | 2020-05-29 | 2023-09-21 | Somnus Scientific Ltd | A propofol sensor |
| CN112362616B (en) * | 2020-11-10 | 2024-05-14 | 重庆理工大学 | Optical fiber sensor for selectively measuring concentration of parachlorophenol |
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| CN201768220U (en) * | 2010-07-01 | 2011-03-23 | 广州市亿福迪医疗器械有限公司 | Propofol and remifentanil intravenous anesthesia injection device |
| CN202133614U (en) * | 2011-07-12 | 2012-02-01 | 新疆医科大学 | Online detecting device capable of detecting fiber sensing molecular imprinting |
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| CN201768220U (en) * | 2010-07-01 | 2011-03-23 | 广州市亿福迪医疗器械有限公司 | Propofol and remifentanil intravenous anesthesia injection device |
| CN202133614U (en) * | 2011-07-12 | 2012-02-01 | 新疆医科大学 | Online detecting device capable of detecting fiber sensing molecular imprinting |
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| 丁海燕.异丙酚生物样品的在线前处理及光纤化学传感分析研究.《中国优秀硕士学位论文全文数据库》.2008, |
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| WO2017133794A1 (en) * | 2016-02-02 | 2017-08-10 | Fresenius Kabi Deutschland Gmbh | Method and device for muasuring a concentration of at least one drug in an exhalation air of a patient |
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