CN211528202U

CN211528202U - Portable pesticide residue detection device based on enzyme inhibition rate method

Info

Publication number: CN211528202U
Application number: CN201920807065.5U
Authority: CN
Inventors: 刘辉; 张爽; 黄钟标; 朱建恩; 张创森; 苏敏仪; 梁泽坤; 黄绍明
Priority date: 2019-05-31
Filing date: 2019-05-31
Publication date: 2020-09-18
Anticipated expiration: 2029-05-31

Abstract

The utility model discloses a portable pesticide residue detection device relates to food detection technology field, include two-layer box about separating into through the baffle, thermostated container and spectrophotometer have been placed on the upper strata of box, the lower floor of box is provided with and can follows the lower part side of box carries out the instrument box of pull, placed in the instrument box and extracted detection instrument such as agent bottle, developer bottle, substrate bottle, enzyme bottle, straw, detection liquid extraction bottle, test tube and cell. The utility model discloses place all detection device and detection instrument in a box, convenient to carry, easy operation is applicable to the measurement personnel and goes out to carry out the witnessed inspections.

Description

Portable pesticide residue detection device based on enzyme inhibition rate method

Technical Field

The utility model relates to a food detection technology field specifically is a portable pesticide residue detection device.

Background

Pesticide residue in fruits is a necessary phenomenon after pesticide application in the fruit planting process, but if the pesticide residue exceeds the maximum residue limit standard, the pesticide residue can have adverse effects on human and livestock or cause the risk of toxicity to organisms in an ecosystem through a food chain, so that the detection of the pesticide residue in the fruits is necessary. The traditional method for detecting the pesticide residue on the surface of the fruit mainly comprises a gas chromatography, a gas chromatography-mass spectrometry, a high performance liquid chromatography, a liquid chromatography-mass spectrometry and the like, and although the methods are developed relatively mature, the problems of complex pretreatment process, long detection time and the like of sample detection exist, and the methods are not beneficial to field detection.

Disclosure of Invention

The utility model provides a portable pesticide residue detection device has solved prior art and has operated complicacy, is unfavorable for the problem of witnessed inspections.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a portable pesticide residue detection device, includes two-layer box, thermostated container and spectrophotometer about separating into through the baffle, box upper portion is provided with the box lid of flip formula, works as the box lid covers during the box, the box with it is fixed through the hasp between the box lid, thermostated container and spectrophotometer have been placed on the upper strata of box, the lower floor of box is provided with and can follows the lower part side of box carries out the toolbox of pull, placed in the toolbox and detect the external member, it draws bottle, test tube and cuvette to detect the external member including extractant bottle, developer bottle, substrate bottle, enzyme bottle, straw, detection liquid, be provided with the bubble cotton in the toolbox, set up the recess of different shapes on the bubble cotton for place the various instruments that detect in the external member.

Further, the cuvette is placed in the cuvette box, the cuvette box is placed in the groove of the foam, and a cuvette cover is further placed in the cuvette box.

Further, the inner diameter L2 of the bottle opening of the detection liquid extraction bottle is 0.5-2mm larger than the inner diameter L1 of the bottle body, a filter screen is arranged at the bottle opening of the detection liquid extraction bottle, and the detection liquid extraction bottle is also provided with a detection liquid extraction bottle cap.

Furthermore, the structure of the filter screen is a frustum-shaped filter screen support body, the lower part of the filter screen support body is connected with a cylindrical filter element, the diameter L3 of the screen opening at the upper part of the filter screen support body is smaller than the inner diameter L2 of the bottle opening of the detection liquid extraction bottle but larger than the inner diameter L1 of the bottle body, and the diameter L4 of the filter element at the lower part of the filter screen is smaller than the inner diameter L1 of the bottle body.

Further, the inside of thermostated container is placed and is placed the support that can place at least 2 the test tube.

Furthermore, a shockproof sponge is placed between the incubator and the spectrophotometer and the box body.

Due to the adoption of the technical scheme, the utility model discloses reach following technological effect:

all detection devices and detection tools are placed in a box body, so that the portable detection device is convenient to carry, simple to operate and suitable for field detection of detection personnel.

Drawings

Fig. 1 is a schematic structural diagram of a portable pesticide residue detection device.

FIG. 2 is a schematic structural diagram of the cuvette and the cuvette box.

FIG. 3 is a schematic view of the structure of the detection liquid extraction bottle.

Fig. 4 is a schematic structural diagram of the filter screen.

In the drawings: 10-box, 11-box cover, 12-hasp, 13-clapboard, 14-shockproof sponge, 20-tool box, 21-handle, 22-foam, 30-incubator, 31-test tube support, 40-spectrophotometer, 50-detection kit, 51-extractant bottle, 52-color reagent bottle, 53-substrate bottle, 54-enzyme bottle, 55-straw, 56-detection liquid extraction bottle, 56 a-detection liquid extraction bottle cap, 56 b-filter screen, 57-test tube, 58-cuvette box, 58 a-cuvette and 58 b-cuvette cover.

Detailed Description

Embodiments of the present invention are further described below with reference to the accompanying drawings.

Referring to fig. 1-4, a portable pesticide residue detection device comprises a box body 10, a thermostat 30 and a spectrophotometer 40, wherein the box body 10 is divided into an upper layer and a lower layer by a partition plate 13, the upper layer of the box body 10 is provided with a flip-type box body cover 11, and when the box body cover 11 covers the box body 10, the box body 10 and the box body cover 11 can be fixed through a hasp 12. Thermostated container 30 and spectrophotometer 40 have been placed to the upper strata of box 10, and the test tube support 31 that can place 2 at least test tubes 57 is placed to the inside of thermostated container 10, can wait to detect a plurality of solutions simultaneously and heat the reaction. The shockproof sponge 14 is placed between the constant temperature box 30 and the spectrophotometer 40 and the box body 10, so that the constant temperature box 30 and the spectrophotometer 40 are prevented from being collided in the carrying process.

The lower layer of the box body 10 is provided with a tool box 20 which can be drawn from the lower side of the box body 10, and a handle 21 is arranged on the side surface of the tool box 20, so that the tool box 20 can be conveniently pushed and pulled. The kit 20 has a detection kit 50 disposed therein, and the detection kit 50 includes an extractant bottle 51, a developer bottle 52, a substrate bottle 53, an enzyme bottle 54, a pipette 55, a detection solution extraction bottle 56, a test tube 57, and a cuvette 58 a. The tool box 20 is provided with a foam 22, and the foam 22 is provided with grooves with different shapes for placing various tools in the detection kit 50. The cuvette 58a is placed in the cuvette box 58 to avoid the cuvette to be contaminated, the cuvette box 58 is placed in the groove of the foam 22, and a cuvette cover 58b is also placed in the cuvette box 58.

The inner diameter L2 of the bottle mouth of the detection liquid extraction bottle 56 is 0.5-2mm larger than the inner diameter L1 of the bottle body, a filter screen 56b is arranged at the bottle mouth of the detection liquid extraction bottle 56, and a detection liquid extraction bottle cap 56a is also arranged at the bottle mouth of the detection liquid extraction bottle 56. The structure of filter screen 56b is the filter screen support body of a frustum form, and the sub-unit connection of filter screen support body has cylindric filter core, and the net gape diameter L3 on the upper portion of filter screen support body is less than the bottleneck internal diameter L2 that detects liquid and draw bottle 56, nevertheless is bigger than bottle internal diameter L1, can guarantee that filter screen 56b places and can not fall into the bottle when detecting the bottleneck department that liquid drawed bottle 56, and net bottom diameter L4 of the lower part of filter screen 56b is than bottle internal diameter L1 is little.

The incubator 30 used in this embodiment is an incubator with adjustable temperature, the temperature adjustment range is 0-60 ℃, and the spectrophotometer 40 used is a digital display ultraviolet spectrophotometer. The incubator and the spectrophotometry are the prior art and are commercially available, and the incubator and the spectrophotometry are respectively provided with a power plug and connected with a 220V power socket when in use. An organic solvent is placed in the extractant bottle 51, acetylthiocholine iodide is placed in the substrate bottle 53, acetylcholinesterase is placed in the enzyme bottle 54, and dithiodinitrobenzoic acid is placed in the developer bottle 52.

The working principle is as follows: taking a small piece of vegetable leaves, wiping off soil on the surface, cutting the small piece of vegetable leaves, putting the small piece of vegetable leaves into a detection liquid extraction bottle 56, adding a certain amount of organic solvent into the detection liquid extraction bottle 56, installing a filter screen 56b, screwing a detection liquid extraction bottle cap 56a, manually shaking the bottle, then opening the detection liquid extraction bottle cap 56a, pouring the solution in the detection liquid extraction bottle 56 into a test tube 57, standing for 2 minutes, taking the supernatant liquid by using a suction tube 55, and putting the supernatant liquid into another test tube 57. Thermostat 30 and spectrophotometer 40 are connected to the socket through power lines, the power switch of thermostat 30 is turned on, the temperature is adjusted to 37 ℃, and preheating is performed. Simultaneously, a certain amount of pure water is added into the color reagent bottle 52, the substrate bottle 53 and the enzyme bottle 54 for dissolving and diluting, a certain amount of enzyme, the color reagent and the substrate are put into a test tube 57 filled with clear liquid to be tested, the test tube 57 is put on a test tube bracket 31 in a constant temperature box for reaction for 10 minutes at 37 ℃, and the color change of the solution in the test tube is observed. If the solution to be detected does not contain pesticides or the pesticide residue is extremely low, the activity of acetylcholinesterase is not inhibited, the substrate, namely the acetylthiocholine iodide, is hydrolyzed, and the hydrolysate and the added color developing agent, namely dithio-bis-nitrobenzoic acid, generate color reaction; on the contrary, the added color reagent dithio-dinitrobenzoic acid does not show color or has little color change. When the pesticide residue of the object to be detected needs to be confirmed again, a certain amount of clear liquid to be detected is taken by a suction pipe 55 and put into a cuvette 58a, the change value of the absorbance at 412nm along with the time is measured by a spectrophotometer 40, the inhibition rate is calculated, and whether the sample contains the residue of the organophosphorus or carbamate pesticide can be judged according to the inhibition rate.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. The utility model provides a portable pesticide residue detection device, includes two-layer box, thermostated container and spectrophotometer about separating into through the baffle, box upper portion is provided with the box lid of flip formula, works as the box lid covers during the box, the box with it is fixed through the hasp between the box lid, its characterized in that, thermostated container and spectrophotometer have been placed on the upper strata of box, the lower floor of box is provided with and can follows the lower part side of box carries out the toolbox of pull, placed in the toolbox and detect the external member, it includes extractant bottle, colour development agent bottle, substrate bottle, enzyme bottle, straw, detects the liquid and draws bottle, test tube and cuvette to detect the external member, be provided with the bubble cotton in the toolbox, set up the recess of different shapes on the bubble cotton for place the various instruments that detect in the external member.

2. The convenient pesticide residue detection device of claim 1, wherein the cuvette is placed in a cuvette box, the cuvette box is placed in the groove of the foam, and a cuvette cover is further placed in the cuvette box.

3. The portable pesticide residue detection device of claim 1, wherein the inner diameter L2 of the bottle opening of the detection liquid extraction bottle is 0.5-2mm larger than the inner diameter L1 of the bottle body, a filter screen is arranged at the bottle opening of the detection liquid extraction bottle, and the detection liquid extraction bottle is further provided with a detection liquid extraction bottle cap.

4. The portable pesticide residue detection device of claim 3, wherein the filter screen is a frustum-shaped filter screen support, a cylindrical filter element is connected to the lower portion of the filter screen support, the diameter L3 of the upper portion of the filter screen support is smaller than the inner diameter L2 of the bottle mouth of the detection liquid extraction bottle but larger than the inner diameter L1 of the bottle body, and the diameter L4 of the lower portion of the filter screen is smaller than the inner diameter L1 of the bottle body.

5. The portable pesticide residue detection device of claim 1, wherein a support for placing at least 2 test tubes is placed inside the incubator.

6. The portable pesticide residue detection device of claim 1, wherein a shock-proof sponge is placed between the incubator, the spectrophotometer and the box body.