CN108865821A - A kind of the nucleic acid isothermal amplification chip and application method of integrated thermal cracking - Google Patents
A kind of the nucleic acid isothermal amplification chip and application method of integrated thermal cracking Download PDFInfo
- Publication number
- CN108865821A CN108865821A CN201810745533.0A CN201810745533A CN108865821A CN 108865821 A CN108865821 A CN 108865821A CN 201810745533 A CN201810745533 A CN 201810745533A CN 108865821 A CN108865821 A CN 108865821A
- Authority
- CN
- China
- Prior art keywords
- chip body
- cracking
- chip
- detection cell
- microchannel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of nucleic acid isothermal amplification chip of integrated thermal cracking, which includes separable cracking chamber chip body, detection cell chip body, connector and buckle structure, is used in conjunction with mating detector；In preceding processing, cracks chamber chip body and detection cell chip body is in different warm areas, pre-treatment high temperature does not impact the reagent prestored below.When closing up cracking chamber chip body and detection cell chip body, the diaphragm seal of the sharp portion insertion cracking chamber chip body of the second microchannel on detection cell chip body, so that the first microchannel is connected to the second microchannel；Chamber chip body and detection cell chip body are cracked by buckle structure clamping, no leakage, being ined succession when separating by connector will not be kept completely separate and cause the problems such as mismatching, obscuring.Whole process not will cause the failure of detection reagent, will not interfere to experimental result, to improve detection efficiency.
Description
Technical field
The present invention relates to micro fluidic chip technical fields, more particularly, to a kind of nucleic acid isothermal amplification core of integrated thermal cracking
Piece and application method.
Background technique
Micro-fluidic chip is the emerging technology that chemistry or biological sample are operated and detected on chip.It is micro-
Fluidic chip can be integrated into the operating units such as the preparation of sample, reaction, separation and detection one several square centimeters of chip
On, by the manipulation and control to fluid in microchannel network, it is automatically performed analytic process.Since microfluidic chip technology has
Sample volume is small, integrated level is high, easy realization automation control and the characteristics of high throughput analysis, so that being given birth on micro-fluidic chip
It is more convenient, quick, low in cost to change the more conventional analysis sample pre-treatments of operation.
Current micro-fluidic chip is substantially integral type, but in practical applications, pre-treatment, reaction, the detection of sample
Deng often using different temperature, and reagent is often prestored in micro-fluidic chip, these reagents are not intended to high temperature, and preceding
It is likely to use high temperature when processing, such integrated chip design exists many difficult.In view of different temperatures can be by mating instrument
Different temperature control regions is arranged in device, and chip different parts are placed in instrument different zones by same chip by separating,
It can be but close when convenient and reagent saves in order to actually use to avoid reaction detection reagent failure thermo-labile behind when pre-treatment
Feng Xing, it is integrated for generally requiring chip.
Nucleic acid isothermal amplification technology is the general name of molecule biology techniques, they can expand at a certain specific temperature
Increase specific DNA or RNA.Requirement of the nucleic acid isothermal amplification to instrument greatly simplifies compared with round pcr, and the reaction time is significantly
Shorten, is more able to satisfy fast and convenient demand.Nucleic acid isothermal amplification technology has portioned product, such as in conjunction with micro-fluidic chip
Pathogenic organisms of respiratory tract kit for detecting nucleic acid difficult to understand is won, but nucleic acid-templated pre-treatment etc. carries out outside, chip integrates
Spend low, manual operation or more complex.Separately there is the report that paramagnetic particle method nucleic acid extraction is integrated in chip, but have no actual product,
Paramagnetic particle method cost is also high.Existing nucleic acid-templated rapid extracting method, such as glass bead method, nucleic acid targeted release agent method, step is simpler
Single, the template of extraction is completely suitable for quickly detecting, but extraction process is required to be heated at high temperature, and detection reagent is not resistant to height
Temperature can fail if reagent is pre-stored on chip, this is a difficult point of the quick detection chip design of nucleic acid.
Summary of the invention
In view of the above problems, the present invention provides a kind of nucleic acid isothermal amplification chip of integrated thermal cracking, and chip is designed to
It is detachable, it is used in conjunction with mating detector, in preceding processing, chip different piece is in different warm areas, and pre-treatment high temperature is not right
The reagent prestored below impacts.The chip both can integration close up, the form of different piece can also be separated into.
The embodiment of the present invention provides a kind of nucleic acid isothermal amplification chip of integrated thermal cracking, including：
Chamber chip body is cracked, the cracking chamber chip body is equipped with sample holes, cracking pond, the first microchannel and is used for close
Seal the diaphragm seal of first microchannel outlet end；
Detection cell chip body, the detection cell chip body are equipped with the second microchannel, detection cell and Micropump interface；It is described
The arrival end of second microchannel is equipped with sharp portion；
Connector, for the cracking chamber chip body and detection cell chip body to link into an integrated entity；
Buckle structure, for the cracking chamber chip body and detection cell chip body to be connected together；When the cracking pond
When chip body and detection cell chip body are connected together, the outlet end of first microchannel and second microchannel
Arrival end is connected.
In one embodiment, one end of the connector is connected on the cracking chamber chip body, and the other end is connected to
On the detection cell chip body；
The connector is telescopic bar or elastic rib.
In one embodiment, the buckle structure, including：Card base and card slot compatible with the card base；
The card base is arranged on the cracking chamber chip body, and the card slot is arranged on the detection cell chip body；
Or
The card base is arranged on the detection cell chip body, and the card slot is arranged on the cracking chamber chip body.
In one embodiment, on the cracking chamber chip body and/or the detection cell chip body is fixed equipped with chip
Part.
In one embodiment, the venthole for connecting the cracking pond is additionally provided on the cracking chamber chip body.
In one embodiment, the material of the cracking chamber chip body and the detection cell chip body is following any one
Kind：
Glass, quartz, polycarbonate, polymethyl methacrylate and dimethyl silicone polymer.
Second aspect, the embodiment of the present invention also provide a kind of user of the nucleic acid isothermal amplification chip of integrated thermal cracking
Method, including：
Step 1：Sample-adding：Prepare liquid is added in the cracking pond of cracking chamber chip body by sample holes, with air-permeable, hydrophobic membrane or
Lid seals sample holes；
Step 2：Chip placement：The chip is put into corresponding detector specific region and carries out cracking processing；
Step 3：Close up chip：Isolated cracking chamber chip body and detection cell chip body are aligned by the detector automatically
Close up, in the diaphragm seal of the sharp portion insertion cracking chamber chip body of detection cell chip body, the first microchannel and the second miniflow are logical
Road connection, passes through buckle structure clamping；
Step 4：Liquid will crack liquid transfer in pond to detection cell or by centrifugation in detector Micropump absorption cracking pond
To detection cell, respective reaction detection is carried out.
Compared with prior art, the beneficial effect of the embodiment of the present invention includes：The chip includes separable cracking pond core
Sheet body and detection cell chip body, are used in conjunction with mating detector, in preceding processing, crack chamber chip body and detection cell chip body is in
Different warm areas, pre-treatment high temperature do not impact the reagent prestored below.The chip both can integration close up, can also divide
From the form at different piece.The different parts of chip are cracked into chamber chip body, detection cell chip body by separation design
It is placed in instrument different zones, the failure of thermo-labile reaction detection reagent below is caused when can be to avoid pre-treatment, thus
Improve the efficiency of detection.
Other features and advantages of the present invention will be illustrated in the following description, also, partly becomes from specification
It obtains it is clear that understand through the implementation of the invention.The objectives and other advantages of the invention can be by written explanation
Specifically noted structure is achieved and obtained in book, claims and attached drawing.
Below by drawings and examples, technical scheme of the present invention will be described in further detail.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings：
Fig. 1 is the nucleic acid isothermal amplification chip seperated schematic diagram of integrated thermal cracking provided in an embodiment of the present invention；
Fig. 2 is structural schematic diagram when Fig. 1 provided in an embodiment of the present invention closes up；
Fig. 3 is the direction A-A chip interface front view in Fig. 1；
The partial enlarged view of interface in Fig. 4 Fig. 1 provided in an embodiment of the present invention；
The nucleic acid isothermal amplification chip seperated schematic diagram of Fig. 5 another integrated thermal cracking provided in an embodiment of the present invention；
Structural schematic diagram when Fig. 6 Fig. 5 provided in an embodiment of the present invention closes up；
Wherein：1- cracks chamber chip body, 11- sample holes, and 12- cracks pond, the first microchannel of 13-, 14- diaphragm seal, 15-
Venthole, 2- detection cell chip body, the second microchannel of 21-, 22- detection cell, 23- Micropump interface, 24- sharp portion, 3- connection
Part, 4 buckle structures, 41- card base, 42- card slot, 5- chip fixing piece.
Specific embodiment
The principle and features of the present invention will be described below with reference to the accompanying drawings, and the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the invention.
The embodiment of the invention provides a kind of nucleic acid isothermal amplification chips of integrated thermal cracking, referring to Fig.1 shown in -2, packet
It includes：Crack chamber chip body 1, detection cell chip body 2, connector 3 and buckle structure 4；
Wherein, cracking chamber chip body 1 is equipped with sample holes 11, cracking pond 12, the first microchannel 13 and diaphragm seal 14, close
Mounting 14 is used to seal the outlet end of the first microchannel 13；
Detection cell chip body 2 is equipped with the second microchannel 21, detection cell 22 and Micropump interface 23；Second microchannel 21
Arrival end be equipped with sharp portion 24；
Above-mentioned two chip body is linked together by connector 3, can play mark action, and it is same to show that the two belongs to
One chip；Interface in cracking chamber chip body and detection cell chip body joint is additionally provided with buckle structure；When pond core will be cracked
When sheet body and detection cell chip body are connected together, the sharp portion of the arrival end of the second microchannel punctures the first microchannel
The diaphragm seal of outlet end realizes the connection of the first microchannel and the second microchannel.
In this implementation, such as the chip for needing temperature zonal control, integrated thermal cracking provided by the invention can be used
Nucleic acid isothermal amplification chip.As nucleic acid constant-temperature amplification detection can apply the nucleic acid isothermal amplification core of integrated thermal cracking of the invention
Piece, when template extraction, often may require that 70~100 DEG C (operating in cracking chamber chip body), and detects temperature and be generally below 70 DEG C
(being operated in detection cell chip body).If detection reagent is also in 70 DEG C or more high temperature (inspection during template extraction
Survey chamber chip body), it most probably fails, result is caused to malfunction.
Therefore, the nucleic acid isothermal amplification chip of integrated thermal cracking of the invention, referring to Fig.1 shown in, be designed to it is split type, and
It can be connected by connector, crack chamber chip body, is i.e. pretreatment part microchannel mouth has diaphragm seal sealing；Referring to Fig. 2
Shown, the sharp portion of the second microchannel mouth of (detection cell chip body) reaction detection part is inserted into pretreatment part when closing up
The diaphragm seal of (cracking chamber chip body), referring to shown in Fig. 3, so that the first microchannel is connected to the second microchannel, i.e.,：So that
Pretreatment part is connected to the microchannel of reaction detection part, cracks chamber chip body and detection cell chip body passes through buckle structure
Clamping, no leakage, being ined succession when separating by connector will not be kept completely separate and cause the problems such as mismatching, obscuring.
I.e.：When high temperature extracts liquid to be detected, detection cell chip body can be far from hot environment；And after the completion of extracting, inspection
When surveying extracting solution, the cracking chamber chip body of separate type and detection cell chip body can be closed up integrally, be completed in low temperature environment
Detection.Whole process not will cause the failure of liquid to be detected, will not interfere to experimental result, to improve detection effect
Rate.
Further, one end of above-mentioned connector 3 is connected on cracking chamber chip body, and the other end is connected to detection chamber chip
On body, connector can be telescopic lever, be also possible to elastic rib；The form of connector is not limited to above two mode,
He can play mark action, show that the two belongs to the connection type or connector of chip, belong to protection of the invention
Range.
It is the enlarged diagram cracked in Fig. 1 at chamber chip body and detection cell chip body interface referring to shown in Fig. 4,
Middle buckle structure 4, including card base 41 and card slot 42 compatible with card base 41；When the setting of card base 41 is on cracking chamber chip body 1
When, card slot 42 is arranged on detection cell chip body 2；Or when card base 41 is arranged on detection cell chip body 2, card slot 42 is arranged
On cracking chamber chip body 1.
After cracking chamber chip body extraction pond to be detected, when needing to carry out subsequent detection, pond will be cracked by buckle structure 4
Chip body 1 and detection cell chip body 2 are fastened togather, while the insertion cracking chamber chip body 1 of sharp portion 24 of detection cell chip body 2
Diaphragm seal 14, realize the first microchannel 13 be connected with the second microchannel 21, guarantee that liquid to be detected will not leak.
Further, referring to shown in Fig. 5-6, the nucleic acid isothermal amplification chip of the integrated thermal cracking is fixed on for convenience
On detector, or clamping chip for convenience, it can be equipped with chip fixing piece 5 on cracking chamber chip body 1, can also detect
Chamber chip body 2 is equipped with chip fixing piece 5；Or it is fixed on cracking chamber chip body 1 and detection cell chip body 2 equipped with chip simultaneously
Part 5.
In the present embodiment, chip fixing piece 5 can be fixation hole as shown in Figure 5, be also possible to fixing groove, can be with
It is the column of protrusion, in the present embodiment, without limitation to the shape of chip fixing piece, size and location, as long as cooperation detection
Instrument or clamping device use, component for fixing.
Further, referring to shown in Fig. 5-6, liquid to be detected is filled from sample holes 11 for convenience, it can be in cracking pond core
Sheet body 1 is equipped with the venthole 15 in connection cracking pond 12.The nucleic acid isothermal amplification of integrated thermal cracking provided in an embodiment of the present invention
Chip, can be using any one in glass, quartz, polycarbonate, polymethyl methacrylate and dimethyl silicone polymer
Material is made.In addition the global shape of the invention to chip without limitation, can be designed to various shape according to specific requirements；Such as
It is round rectangle referring to Fig.1 shown in -2 when Micropump controls the micro-fluidic chip of feed liquor；For another example it is centrifugal separate it is micro-fluidic
It is almost fan when closing up referring to shown in Fig. 5-6 when chip.According further to the specific detection function of chip, can be set on chip
Set multiple cracking ponds or detection cell, the embodiment of the present invention to this also without limitation.
Second aspect, the present invention also provides the application methods of the nucleic acid isothermal amplification chip of integrated thermal cracking：
Embodiment 1：
By taking the micro-fluidic chip of Micropump control feed liquor as an example, the nucleic acid such as integrated thermal cracking provided by the invention may be designed to
Isothermal duplication chip, referring to Fig.1 shown in -2, the global shape of chip is round rectangle.
Its application method is as follows：
1, it is loaded：By prepare liquid by sample holes be added micro-fluidic chip cracking pond in, with air-permeable, hydrophobic membrane or lid seal into
Sample hole；
2, chip placement：Micro-fluidic chip is put into corresponding detector specific region and carries out cracking processing；
3, close up chip：Detector automatically closes up isolated chip part alignment, before the microchannel of reaction detection part
In the diaphragm seal for holding sharp portion insertion pretreatment part, channel is connected to, and chip card buckle is tight；
4, detector Micropump draws liquid in cracking pond and carries out respective reaction detection to detection cell.
Embodiment 2：
By taking centrifugal separable micro-fluidic chip as an example, the nucleic acid of integrated thermal cracking such as provided by the invention may be designed to
Warm amplification chip, referring to shown in Fig. 5-6, the global shape of chip is almost fan.
Using steps are as follows：
1, it is loaded：By prepare liquid by sample holes be added micro-fluidic chip cracking pond in, with air-permeable, hydrophobic membrane or lid seal into
Sample hole and venthole；
2, chip placement：Micro-fluidic chip is put into corresponding detector specific region and carries out cracking processing；
3, close up chip：Detector automatically closes up isolated chip alignment, reaction detection part microchannel front end point
In the diaphragm seal of head insertion pretreatment part, channel connection buckles clamping；
4, liquid in cracking pond is transferred to detection cell by centrifugation by detector, is accordingly detected.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (7)
1. a kind of nucleic acid isothermal amplification chip of integrated thermal cracking, which is characterized in that including：
It cracks chamber chip body (1), the cracking chamber chip body (1) is logical equipped with sample holes (11), cracking pond (12), the first miniflow
Road (13) and diaphragm seal (14) for sealing the first microchannel (13) outlet end；
Detection cell chip body (2), the detection cell chip body (2) are equipped with the second microchannel (21), detection cell (22) and micro-
Pump interface (23)；The arrival end of second microchannel (21) is equipped with sharp portion (24)；
Connector (3), for the cracking chamber chip body (1) and detection cell chip body (2) to link into an integrated entity；
Buckle structure (4), for the cracking chamber chip body (1) and detection cell chip body (2) to be connected together；It is split when described
Solution chamber chip body (1) and detection cell chip body (2) be when being connected together, the outlet end of first microchannel (13) with it is described
The arrival end of second microchannel (21) is connected.
2. a kind of nucleic acid isothermal amplification chip of integrated thermal cracking according to claim 1, which is characterized in that the connection
One end of part (3) is connected on the cracking chamber chip body (1), and the other end is connected on the detection cell chip body (2)；
The connector (3) is telescopic bar or elastic rib.
3. a kind of nucleic acid isothermal amplification chip of integrated thermal cracking according to claim 1, which is characterized in that the buckle
Structure (4), including：Card base (41) and the compatible card slot (42) with the card base (41)；
The card base (41) is arranged on the cracking chamber chip body (1), and the card slot (42) is arranged in the detection chamber chip
On body (2)；
Or
The card base (41) is arranged on the detection cell chip body (2), and the card slot (42) is arranged in the cracking chamber chip
On body (1).
4. a kind of nucleic acid isothermal amplification chip of integrated thermal cracking according to claim 1, which is characterized in that the cracking
On chamber chip body (1) and/or the detection cell chip body (2) is equipped with chip fixing piece (5).
5. a kind of nucleic acid isothermal amplification chip of integrated thermal cracking according to claim 1, which is characterized in that the cracking
The venthole (15) for connecting cracking pond (12) is additionally provided on chamber chip body (1).
6. a kind of nucleic acid isothermal amplification chip of integrated thermal cracking according to claim 1-5, which is characterized in that
It is described cracking chamber chip body (1) and the detection cell chip body (2) material for it is following any one：
Glass, quartz, polycarbonate, polymethyl methacrylate and dimethyl silicone polymer.
7. a kind of application method of the nucleic acid isothermal amplification chip of integrated thermal cracking, which is characterized in that including：
Step 1：Sample-adding：Prepare liquid is added in the cracking pond of cracking chamber chip body by sample holes, is sealed with air-permeable, hydrophobic membrane or lid
Firmly sample holes；
Step 2：Chip placement：The chip is put into corresponding detector specific region and carries out cracking processing；
Step 3：Close up chip：The detector automatically closes up isolated cracking chamber chip body and the alignment of detection cell chip body,
In the diaphragm seal of the sharp portion insertion cracking chamber chip body of detection cell chip body, the first microchannel and the second microchannel connect
It is logical, pass through buckle structure clamping；
Step 4：Detector Micropump draw cracking pond in liquid to detection cell or by centrifugation will cracking pond in liquid be transferred to inspection
Pond is surveyed, respective reaction detection is carried out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810745533.0A CN108865821B (en) | 2018-07-09 | 2018-07-09 | Nucleic acid isothermal amplification chip integrated with thermal cracking and use method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810745533.0A CN108865821B (en) | 2018-07-09 | 2018-07-09 | Nucleic acid isothermal amplification chip integrated with thermal cracking and use method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108865821A true CN108865821A (en) | 2018-11-23 |
| CN108865821B CN108865821B (en) | 2021-07-02 |
Family
ID=64300235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810745533.0A Active CN108865821B (en) | 2018-07-09 | 2018-07-09 | Nucleic acid isothermal amplification chip integrated with thermal cracking and use method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108865821B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112414983A (en) * | 2020-11-05 | 2021-02-26 | 北京中科生仪科技有限公司 | Biological detection method based on excitation light source |
| CN113174307A (en) * | 2021-01-15 | 2021-07-27 | 北京中科生仪科技有限公司 | Upper cover based on nucleic acid detection chip installation bin |
| CN113175466A (en) * | 2021-01-29 | 2021-07-27 | 北京中科生仪科技有限公司 | Fastening device |
| CN114113567A (en) * | 2020-11-26 | 2022-03-01 | 深圳市理邦精密仪器股份有限公司 | Molecular diagnosis centrifugal test card |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060246580A1 (en) * | 2005-05-02 | 2006-11-02 | Jin-Tae Kim | Polymerase chain reaction module, and multiple polymerase chain reaction system including the module |
| CN103103120A (en) * | 2013-01-18 | 2013-05-15 | 上海交通大学 | Integrated temperature controlled PCR-CE (polymerase chain reaction-capillary electrophoresis) micro-fluidic chip and preparation method thereof |
| KR101306341B1 (en) * | 2006-09-05 | 2013-09-09 | 삼성전자주식회사 | Centrifugal force-based microfluidic device for nucleic acid extraction from biological sample and microfluidic system comprising the microfluidic system |
| CN104312913A (en) * | 2014-10-17 | 2015-01-28 | 复旦大学附属华山医院 | Microchip capable of visually detecting tumor gene mutation by integrating extraction and amplification of whole blood nucleic acid and application of microchip |
| CN106563517A (en) * | 2016-10-26 | 2017-04-19 | 杭州霆科生物科技有限公司 | Micro-fluidic chip and detection system for detecting formaldehyde and pH value of textile |
| CN206762905U (en) * | 2017-04-07 | 2017-12-19 | 杭州霆科生物科技有限公司 | A kind of micro-fluidic chip and system for the detection of textile triclosan |
| CN107513495A (en) * | 2017-09-01 | 2017-12-26 | 中国科学院苏州生物医学工程技术研究所 | Multichannel droplet detection chip for detection of nucleic acids |
| CN107619781A (en) * | 2016-07-15 | 2018-01-23 | 中国检验检疫科学研究院 | A kind of single reaction temperature control high flux micro-fluidic chip nucleic acid amplifier |
| CN107921431A (en) * | 2015-04-07 | 2018-04-17 | 源鉴定私人有限公司 | fluid chip |
-
2018
- 2018-07-09 CN CN201810745533.0A patent/CN108865821B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060246580A1 (en) * | 2005-05-02 | 2006-11-02 | Jin-Tae Kim | Polymerase chain reaction module, and multiple polymerase chain reaction system including the module |
| KR101306341B1 (en) * | 2006-09-05 | 2013-09-09 | 삼성전자주식회사 | Centrifugal force-based microfluidic device for nucleic acid extraction from biological sample and microfluidic system comprising the microfluidic system |
| CN103103120A (en) * | 2013-01-18 | 2013-05-15 | 上海交通大学 | Integrated temperature controlled PCR-CE (polymerase chain reaction-capillary electrophoresis) micro-fluidic chip and preparation method thereof |
| CN104312913A (en) * | 2014-10-17 | 2015-01-28 | 复旦大学附属华山医院 | Microchip capable of visually detecting tumor gene mutation by integrating extraction and amplification of whole blood nucleic acid and application of microchip |
| CN107921431A (en) * | 2015-04-07 | 2018-04-17 | 源鉴定私人有限公司 | fluid chip |
| CN107619781A (en) * | 2016-07-15 | 2018-01-23 | 中国检验检疫科学研究院 | A kind of single reaction temperature control high flux micro-fluidic chip nucleic acid amplifier |
| CN106563517A (en) * | 2016-10-26 | 2017-04-19 | 杭州霆科生物科技有限公司 | Micro-fluidic chip and detection system for detecting formaldehyde and pH value of textile |
| CN206762905U (en) * | 2017-04-07 | 2017-12-19 | 杭州霆科生物科技有限公司 | A kind of micro-fluidic chip and system for the detection of textile triclosan |
| CN107513495A (en) * | 2017-09-01 | 2017-12-26 | 中国科学院苏州生物医学工程技术研究所 | Multichannel droplet detection chip for detection of nucleic acids |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112414983A (en) * | 2020-11-05 | 2021-02-26 | 北京中科生仪科技有限公司 | Biological detection method based on excitation light source |
| CN114113567A (en) * | 2020-11-26 | 2022-03-01 | 深圳市理邦精密仪器股份有限公司 | Molecular diagnosis centrifugal test card |
| CN113174307A (en) * | 2021-01-15 | 2021-07-27 | 北京中科生仪科技有限公司 | Upper cover based on nucleic acid detection chip installation bin |
| CN113175466A (en) * | 2021-01-29 | 2021-07-27 | 北京中科生仪科技有限公司 | Fastening device |
| CN113175466B (en) * | 2021-01-29 | 2022-06-28 | 北京中科生仪科技有限公司 | Fastening device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108865821B (en) | 2021-07-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108865821A (en) | A kind of the nucleic acid isothermal amplification chip and application method of integrated thermal cracking | |
| CN101990516B (en) | Multiplex sample preparation system and the use in integrated analysis system thereof | |
| US11813609B2 (en) | Microfluidic cartridge for molecular diagnosis | |
| CN110331089B (en) | Full-automatic nucleic acid extraction amplification detection micro-fluidic chip box and application thereof | |
| US20210011046A1 (en) | Sample processing and detecting kit with a material transfer structure | |
| JP5887041B2 (en) | Pressurizable cartridge for polymerase chain reaction | |
| CN110226089A (en) | Automation on-site testing device and its application method for complex sample processing | |
| JP2018173414A (en) | Test Cartridge with Integrated Transfer Module | |
| KR20140095342A (en) | Micro-fluidic system for analysis of nucleic acid | |
| CN105349401A (en) | Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof | |
| Brooks et al. | Macro-to-micro interfacing to microfluidic channels using 3D-printed templates: application to time-resolved secretion sampling of endocrine tissue | |
| CN216149780U (en) | In vitro diagnostic and analytical device and reagent cartridge | |
| WO2006036757A2 (en) | High temperature flow-through device for rapid solubilization and analysis | |
| US10441952B2 (en) | Device for preparing and/or treating a biological sample | |
| CN215906212U (en) | Nucleic acid amplification reactor | |
| WO2020107641A1 (en) | Biological reaction device provided with microfluidic or nanofluidic structure | |
| US11739288B2 (en) | Plate | |
| CN104818213B (en) | Sliding type closed pathogen detection card box | |
| CN111394234A (en) | Digital chip and method for nucleic acid amplification | |
| WO2022152244A1 (en) | Loop-mediated isothermal amplification chip | |
| CN115814864A (en) | In vitro diagnostic and analytical device and reagent cartridge | |
| KR20190095079A (en) | A preprocessing kit for molecular diagnosis | |
| US20030224371A1 (en) | Integrated cartridge for sample manipulation | |
| CN113164957B (en) | Vacuum assisted drying of filters in microfluidic systems | |
| US20240131511A1 (en) | Microfluidic cartridge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |