CN113175466B - Fastening device - Google Patents

Fastening device Download PDF

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Publication number
CN113175466B
CN113175466B CN202110130185.8A CN202110130185A CN113175466B CN 113175466 B CN113175466 B CN 113175466B CN 202110130185 A CN202110130185 A CN 202110130185A CN 113175466 B CN113175466 B CN 113175466B
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layer
buckle
sample
clamping
sample adding
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CN113175466A (en
Inventor
蔡亦梅
王宏伟
苏海龙
任鲁风
任玮鹏
范东雨
高静
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Beijing Integrated Biosystems Co ltd
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Beijing Integrated Biosystems Co ltd
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    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16B—DEVICES FOR FASTENING OR SECURING CONSTRUCTIONAL ELEMENTS OR MACHINE PARTS TOGETHER, e.g. NAILS, BOLTS, CIRCLIPS, CLAMPS, CLIPS OR WEDGES; JOINTS OR JOINTING
    • F16B1/00—Devices for securing together, or preventing relative movement between, constructional elements or machine parts
    • F16B1/02—Means for securing elements of mechanisms after operation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16B—DEVICES FOR FASTENING OR SECURING CONSTRUCTIONAL ELEMENTS OR MACHINE PARTS TOGETHER, e.g. NAILS, BOLTS, CIRCLIPS, CLAMPS, CLIPS OR WEDGES; JOINTS OR JOINTING
    • F16B1/00—Devices for securing together, or preventing relative movement between, constructional elements or machine parts
    • F16B1/02—Means for securing elements of mechanisms after operation
    • F16B1/04—Means for securing elements of mechanisms after operation disengaged by movement of the actuating member of the element

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Mechanical Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a buckle device, which comprises a fixed frame, a bin gate buckle, a transmission mechanism and a key, wherein one end of the transmission mechanism is provided with a strip hole, and the key is connected with the strip hole of the transmission mechanism through a connecting shaft; the bin gate buckle includes buckle driving piece and buckle follower, and the buckle driving piece includes first meshing portion, first card-tight part and connection tongue, connects the tongue and is connected with the driving medium, and the buckle follower includes second meshing portion, second card-tight part and first button spring, and first meshing portion and the meshing of second meshing portion, the connecting axle of button removes in rectangular hole, drives drive mechanism and rotates, and drive mechanism drives and connects the tongue. First card-tight part and first bellying parallel and level after the separation, second card-tight part and the protruding parallel and level of second after the separation, first meshing portion and the reverse meshing of second meshing portion this moment will set up the detection chip jack-up above that, opens the upper cover this moment and just can take out detection chip, convenient and fast, labour saving and time saving.

Description

Fastening device
Technical Field
The invention relates to the field of nucleic acid detection equipment, in particular to a buckle device.
Background
In the process of nucleic acid detection, a sample needs to be extracted, purified and amplified, so as to obtain the specificity of nucleic acid substances in the sample. Generally, the extraction process of nucleic acid is to mix a sample and a lysis solution to separate nucleic acid substances from impurities such as protein in the sample to obtain a mixture of the nucleic acid substances and the protein, then clean the mixture, adsorb the nucleic acid substances on the surface of magnetic beads by using molecular acting force between the nucleic acid substances and the magnetic beads, clean after adsorption, clean and wash away the impurities such as the protein, and only retain the nucleic acid substances. And finally, separating the nucleic acid substances from the magnetic beads by using the eluent, and allowing the nucleic acid substances to enter an amplification bin so as to perform amplification reaction.
The chip locking device provided by the embodiment of the invention can further comprise a chip positioning sheet, the chip positioning sheet is in a polygon shape with multiple symmetry, the side surface of the chip positioning sheet is provided with a lug, the chip to be detected is arranged on the chip positioning sheet, and the chip positioning sheet can fix the position of the chip to be detected and prevent the chip to be detected from moving in the through hole.
However, since the chip is a consumable material, the chip in the chip bin needs to be replaced, and the replacement of the chip in the related art is time-consuming.
Disclosure of Invention
Therefore, the invention provides the buckle device which can rapidly replace the chip and save the replacement time.
To achieve the above object, the present invention provides a buckle device including: the bin gate buckle, the transmission mechanism and the key are arranged on the inner side of the fixing frame, one end of the transmission mechanism is provided with a long hole, and the key is connected with the long hole of the transmission mechanism through a connecting shaft;
The bin gate buckle comprises a buckle driving piece and a buckle driven piece, the buckle driving piece and the driven piece are arranged oppositely, the buckle driving piece comprises a first meshing part, a first clamping part and a connecting tongue, the connecting tongue is connected with the transmission piece, the buckle driven piece comprises a second meshing part, a second clamping part and a first torsion spring, the buckle driven piece is connected with the fixed frame through the first torsion spring, the first meshing part is meshed with the second meshing part, and the first clamping part is matched with the second clamping part to clamp the detection chip;
the detection chip comprises an upper sample adding layer, a lower gasket and a pipeline layer, wherein the upper sample adding layer is arranged at the top end of the detection chip;
wherein,
the upper side of the sample adding layer is provided with a sample adding hole for adding a sample into the chip, and the sample injected into the chip is subjected to nucleic acid extraction, purification and amplification reaction in sequence;
the sample adding layer and the pipeline layer are movably connected with a limiting frame arranged on the side part of the pipeline layer through a clamping strip, correspondingly, a first clamping groove is arranged on the inner side of the limiting frame and is mutually matched and connected through the clamping strip, so that the relative position switching and fixing of the sample adding layer and the pipeline layer are realized;
The second clamping groove is arranged on the inner side of the limiting frame and is positioned below the first clamping groove;
when in transportation or storage, the sample adding layer is connected with the first clamping groove;
when the sample injection device is used, the gasket is pulled out, the sample injection layer is pressed downwards to be connected with the second clamping groove, and meanwhile, the puncture needle is arranged on the upright column on the pipeline layer and is used for puncturing the reagent arranged in the sample injection layer so as to enable the reagent and the sample to be mixed and reacted;
after the sample adding layer and the pipeline layer are pressed, a first strain gauge arranged at the bottom of the sample adding layer detects the extrusion force between the sample adding layer and the pipeline layer so as to determine the stress uniformity of the sample adding layer and the pipeline layer in the pressing process;
when horizontal acting force is applied to the key, the connecting shaft of the key moves in the elongated hole to drive the transmission mechanism to rotate, the transmission mechanism drives the connecting tongue, and the connecting tongue enables the first engaging part to be engaged reversely so as to realize the separation of the first clamping part and the second clamping part;
and determining the clamping degree of the first clamping part and the second clamping part according to the stress detected by the first strain gauge.
Furthermore, the transmission mechanism comprises a first connecting rod and a second connecting rod, the first connecting rod comprises a first long rod, a first short rod and a first snap spring, the first long rod is rotatably connected with the first short rod, a first transition step surface is arranged between the first long rod and the first short rod, a first clamp spring is welded and fixed with the first long rod, the first short rod is provided with the strip hole, the second connecting rod comprises a second long rod, a second short rod, a second snap spring and a second torsion spring, the second long rod is rotatably connected with the second short rod, a second transition step surface is arranged between the second long rod and the second short rod, a second clamp spring is welded and fixed with the second long rod, the first long rod is provided with a strip-shaped hole, the first long rod is connected with the second short rod, one end of the second torsion spring is fixed on the fixing frame, and the other end of the second torsion spring is connected with the second long rod.
Further, still including the pressure spring, the button includes button cap and depression bar, the pressure spring sets up the downside of depression bar, the depression bar is used for transmitting the effort, the pressure spring sets up the downside of depression bar for reduce with the contact effort in chip storehouse, the connection tongue includes straight arm and bellying, straight arm with bellying integrated into one piece, also be provided with the arch on the second stock first clamping part with the open in-process of second clamping part, protruding along the curvature of bellying slides, first meshing part with the bottom of second meshing part is the plane, first meshing part with second meshing part all includes the crisscross sawtooth that sets up of a plurality of.
Further, the buckle driver further comprises a first stopper disposed between the connection tongue and the first engagement portion to restrict a stroke of the buckle driver during movement of the buckle driver;
the snap follower further comprises a second backstop for constraining travel of the snap follower during movement of the snap follower.
Further, be provided with two lugs on the mount, first chucking part with second chucking part is arranged in respectively when opening in the lug, work as when first chucking part with the second chucking part is closed, first chucking part with the portion of bending of second chucking part stretches out the lug, the portion of bending with detect the chip contact.
Furthermore, the detection chip further comprises a sealing film and a second strain gauge, the sealing film is arranged on the lower side of the pipeline layer and used for realizing sealing, and the second strain gauge is respectively arranged in the first clamping groove and the second clamping groove; taking M positions in the first card slot transversely, detecting the stress at the M positions by the second strain gauge, marking as a first stress function F (F1, F2 … … fm), wherein the selected positions in the second card slot correspond to the positions in the first card slot one by one, and the second stress function of the second card slot is F '(F1', F2 ', … … fm'), and judging the position of the sample adding layer according to the first stress function and the second stress function;
When the sample adding layer is connected with the first card slot, firstly, comparing f1 with fm to obtain a first positive difference value, and if the first positive difference value is higher than a first preset difference value f0, readjusting the sample adding layer; if the first positive difference is lower than a first preset difference f0, performing subsequent operation;
when the sample adding layer is connected with the second clamping groove, f1 ' and fm ' are compared to obtain a second positive difference value, and if the second positive difference value is higher than a second preset difference value f0 ', the sample adding layer is readjusted; if the second positive difference is lower than a second predetermined difference f 0', the subsequent operation is performed.
Further, when the sample adding layer is connected to the second card slot, comparing absolute values of stress difference values at positions corresponding to one another in a first stress function F (F1, F2 … … fm) and a second stress function F '(F1', F2 ', … … fm'), where the first stress function F (F1, F2 … … fm) is a function generated when the sample adding layer is connected to the first card slot, determining whether the absolute value of each stress difference value is smaller than a preset standard error F0, if so, continuing to operate, and if not, determining a corresponding position of the absolute value of a corresponding group of stress difference values, so as to determine the damage of the sample adding layer or the pipeline layer.
Furthermore, a sliding rail is arranged on the lower side of the gasket, correspondingly, a sliding groove is arranged on the upper side surface of the pipeline layer, and the sliding rail is connected with the sliding groove in a matched manner so as to realize the sliding connection between the gasket and the pipeline layer;
the sliding groove is arranged on the inner side of the limiting frame on the pipeline layer;
the end part of the gasket is provided with a plurality of notches and bulges which are arranged at intervals, wherein the slide rail is arranged on the bottom surface of the outermost bulge.
Furthermore, a plurality of sample adding bins are arranged at intervals below the sample adding hole, a reagent outlet is arranged at the end part of each sample adding bin, and a sealing structure is arranged between each reagent outlet and each sample adding bin and used for sealing;
a pressurizing structure is arranged on one side of the sample adding bin and comprises a tube wall, a piston is arranged in the tube wall, and the piston moves back and forth along the tube wall to push the reagent in the piston to flow out or be drawn back to a reagent outlet; and a sealing ring is arranged at the end part of the piston rod of the piston and used for sealing.
Furthermore, the piston rod is also provided with a nut which is in threaded connection with the nut, a guide sleeve is sleeved outside the nut, a corresponding shaft shoulder is arranged inside the pipe wall and used for positioning and fixing the guide sleeve, and clamping rings are arranged outside two ends of the guide sleeve and used for clamping the corresponding guide sleeve; and a sheath is arranged on the outer side of the guide sleeve and used for protecting the piston rod, the screw cap and the guide sleeve.
Compared with the prior art, the invention has the beneficial effects that when horizontal acting force is applied to the key, the connecting shaft of the key moves in the elongated hole to drive the transmission mechanism to rotate, the transmission mechanism drives the connecting tongue, the connecting tongue enables the first engaging part to be reversely engaged, the first clamping part and the second clamping part are separated, the separated first clamping part is flush with the first protruding part, the separated second clamping part is flush with the second protruding part, and the first engaging part and the second engaging part are reversely engaged to jack up a chip arranged on the first clamping part, so that the chip can be taken out by opening the upper cover, the operation is convenient and fast, and the time and the labor are saved.
Especially, drive mechanism realizes carrying out the effect of transmitting the effort that receives on the button for the effort of pressing to button horizontal direction turns into the removal of second stock and connecting tongue upper and lower longitudinal direction, and then realizes the separation of first joint spare and second joint spare, and then makes the detection chip of arranging in between first joint spare and the second joint spare released, is convenient for take out.
Especially, the pressure spring sets up the downside at the depression bar, realizes the soft contact in buckle device and chip storehouse, because change the in-process that detects the chip many times, need open the upper cover that closes the chip storehouse many times, through setting up the pressure spring for in the in-process that opens and shuts, reduce the impact force to the chip storehouse that opens and shuts, protection buckle device prolongs the life in buckle device and chip storehouse.
Especially, along with the curved surface upwards carries out gliding in-process, when upwards to the summit, first chucking part and second chucking part chucking chip realize detecting chip's fixed, when the arch slides down along the bellying, first chucking part and second chucking part slowly open, realize detecting chip's the loosening, are convenient for detect taking out of chip.
Drawings
Fig. 1 is a schematic structural view of a state of a fastening device according to an embodiment of the present invention;
fig. 2 is a schematic structural view of the fastening device according to another state provided in the embodiment of the present invention;
fig. 3 is a schematic structural diagram of a fastening device according to an embodiment of the present invention;
fig. 4 is a schematic view of a buckle device according to an embodiment of the present invention;
FIG. 5 is a schematic perspective view of a detection chip for nucleic acid detection according to an embodiment of the present invention;
FIG. 6 is a schematic diagram showing an exploded structure of a detection chip for nucleic acid detection according to an embodiment of the present invention;
FIG. 7 is a schematic structural diagram of a sample-adding layer according to an embodiment of the present invention;
fig. 8 is a schematic structural diagram of a pipeline layer according to an embodiment of the invention.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described below with reference to examples; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and do not limit the scope of the present invention.
It should be noted that in the description of the present invention, the terms of direction or positional relationship indicated by the terms "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Referring to fig. 1 to 4, a fastening device provided in an embodiment of the present invention includes a fixing frame 1-1, a door fastener, a transmission mechanism, and a key 2-1, where the door fastener, the transmission mechanism, and the key 2-1 are all disposed on an inner side of the fixing frame 1-1, one end of the transmission mechanism is provided with a long hole, and the key 2-1 is connected to the long hole of the transmission mechanism through a connecting shaft 3-1; the bin gate buckle comprises a buckle driving part 6-1 and a buckle driven part 7-1, the buckle driving part 6-1 and the buckle driven part are arranged oppositely, the buckle driving piece 6-1 comprises a first engaging part, a first clamping part and a connecting tongue, the connecting tongue is connected with the transmission piece, the buckle follower 7-1 comprises a second engaging part, a second clamping part and a first torsion spring 8-1, the buckle follower 7-1 is connected with the fixed frame 1-1 through the first torsion spring 8-1, the snap follower is fixed in the axial direction through the snap spring 9-1, the first meshing part is meshed with the second meshing part, the first clamping part and the second clamping part are matched to clamp the upper cover of the chip, and a detection chip is arranged in a clamping seat on the upper cover of the chip; when horizontal acting force is applied to the key 2-1, the connecting shaft 3-1 of the key 2-1 moves in the strip hole to drive the transmission mechanism to rotate, the transmission mechanism drives the connecting tongue, and the connecting tongue enables the first engaging part to be engaged reversely, so that the first clamping part and the second clamping part are separated.
Specifically, the fastening device provided by the embodiment of the invention is applied to a nucleic acid detection PCR instrument, wherein an excitation light source is used for irradiating a nucleic acid chip in the PCR instrument to realize extraction, purification and amplification reactions in the nucleic acid chip, and after one reaction is finished, the detection chip needs to be replaced to perform extraction, purification and amplification reactions in another experiment process.
In practical application, the fastening device comprises a fixed frame 1-1, a door fastener, a transmission mechanism and a key 2-1, wherein the door fastener, the transmission mechanism and the key 2-1 are all arranged on the inner side of the fixed frame 1-1, the fixed frame 1-1 is a carrier of other components, is in a strip shape and is arranged on the inner side of a chip bin, part of components of the fastening device are arranged in the chip bin, the fixed frame 1-1 comprises a first platform part, a first bulge part, a second platform part, a second bulge part and a bending part, the door fastener is arranged between the first bulge part and the second bulge part, the transmission mechanism is arranged at the bending part, the bending part of the bending part is the arrangement position of the second snap spring, the key 2-1 is arranged at the tail end of the bending part, when a horizontal acting force is applied to the key 2-1 in use, connecting axle 3-1 of button 2-1 is in the rectangular downthehole removal drives drive mechanism rotates, drive mechanism drives the connection tongue, the connection tongue makes the reverse meshing of first engaging part, realizes first clamping part with the separation of second clamping part, first clamping part and first bellying parallel and level after the separation, the protruding parallel and level of second clamping part and second after the separation, first engaging part and the reverse meshing of second engaging part this moment will set up the upper cover jack-up on the chip above that, and it just can take out the detection chip to open the upper cover of chip this moment, convenient and fast, labour saving and time saving.
Particularly, the transmission mechanism comprises a first connecting rod 4-1 and a second connecting rod 5-1, the first connecting rod 4-1 comprises a first long rod, a first short rod and a first clamp spring, the first long rod is rotatably connected with the first short rod, a first transition step surface is arranged between the first long rod and the first short rod, the first clamp spring is welded and fixed with the first long rod, the strip hole is arranged on the first short rod, the second connecting rod 5-1 comprises a second long rod, a second short rod, a second clamp spring and a second torsion spring 10-1, the second long rod is rotatably connected with the second short rod, a second transition step surface is arranged between the second long rod and the second short rod, the second clamp spring is welded and fixed with the second long rod, a strip hole is arranged on the first long rod, the first long rod is connected with the second short rod, one end of the second torsion spring 10-1 is fixed on the fixing frame, the other end of the second torsion spring 10-1 is connected with the second long rod.
Particularly, the transmission mechanism achieves the effect of transmitting the acting force received by the key 2-1, so that the pressing acting force in the horizontal direction of the key 2-1 is converted into the movement of the second long rod and the connecting tongue in the vertical direction, the first clamping piece and the second clamping piece are separated, and the chip arranged between the first clamping piece and the second clamping piece is released and is convenient to take out.
Specifically, the key comprises a pressure spring 11-1, the key 2-1 comprises a key cap and a pressure lever, and the pressure spring 11-1 is arranged on the lower side of the pressure lever. Through setting up the key cap, conveniently exert the effort to button 2-1, the depression bar transmits horizontal effort, and pressure spring 11-1 sets up the downside at the depression bar, realizes the soft contact in buckle device and chip storehouse, because in the in-process of changing the detection chip many times, need open the upper cover that closes the chip storehouse many times, through setting up pressure spring 11-1 for in the in-process that opens and shuts, reduce the impact force to the chip storehouse that opens and shuts, protect buckle device, the life in extension buckle device and chip storehouse.
Particularly, the connecting tongue includes straight arm and bellying, straight arm with bellying integrated into one piece, also be provided with the arch on the second stock first chucking part with the open in-process of second chucking part, the arch is along the curvature of bellying slides.
Particularly, the connecting tongue includes straight arm and bellying, and the arch that sets up on the second stock is smooth activity along the curvature of the bellying of connecting tongue for opening and shutting of first chucking part and second chucking part is more smooth, and gentle. Along with the curved surface upwards carries out gliding in-process, when upwards to the summit, first chucking part and second chucking part chucking chip realize the fixed of chip, when the arch when sliding down along the bellying, first chucking part and second chucking part slowly open, realize loosening of chip, the taking out of the chip of being convenient for.
The curvature of the protruding part of protruding along the connecting tongue on the second stock slides for opening and closing of first chucking part and second chucking part is gentler slow, reduces the influence of opening or closing the rigid force of in-process to the chip, makes the contact of first chucking part and second chucking part and chip more gentle, prevents the damage to the chip.
In particular, the snap drive 6-1 further comprises a first endrun stop arranged between the connection tongue and the engagement portion for restricting the stroke of the snap drive 6-1 during the movement of the snap drive 6-1; the snap follower 7-1 further comprises a second stopper to restrict the stroke of the snap follower 7-1 during the movement of the snap follower 7-1.
According to the buckle device provided by the embodiment of the invention, the rotation ranges of the first clamping piece and the second clamping piece are limited by arranging the first stop piece and the second stop piece, the buckle driving piece 6-1 comprises a first engaging part, a first clamping part and a connecting tongue, the first stop piece is arranged between the connecting tongue and the engaging part, the buckle driven piece 7-1 further comprises the second stop piece, the rotation of the buckle driving piece and the rotation of the buckle driven piece 7-1 are limited by the first stop piece and the second stop piece respectively, and the clamp springs arranged on the buckle driving piece and the buckle driven piece 7-1 limit the axial movement of the buckle driving piece and the buckle driven piece 7-1, so that the rotation of the buckle driving piece and the buckle driven piece 7-1 is more stable, and the stable rotation of the buckle driving piece and the buckle driven piece 7-1 is facilitated.
Particularly, the fixing frame 1-1 is provided with two convex blocks, the first clamping part and the second clamping part are arranged in the convex blocks when the first clamping part and the second clamping part are opened, when the first clamping part and the second clamping part are closed, the bending part of the first clamping part and the bending part of the second clamping part extend out of the convex blocks, and the bending part is in contact with the detection chip.
Particularly, be provided with two lugs on mount 1-1, it is first bellying and second bellying respectively, first chucking part with second chucking part is arranged in respectively when opening in the bellying, works as first chucking part with when second chucking part is closed, first chucking part with the portion of bending of second chucking part stretches out the lug through the cooperation of first bellying and first chucking part to and the cooperation of second bellying and second chucking part, realize the chucking to the chip and loosen, convenient and fast carries out the fixing and the release of chip, the change of the chip of being convenient for, improves experimental efficiency.
Specifically, the bottom end of the meshing part is a plane, and the meshing part is a plurality of staggered sawteeth.
In the buckle device provided by the embodiment of the invention, the bottom end of the meshing part is a plane, so that the meshing part can be better matched with the bottom surface of the chip bin, and in the process of rotating the buckle driving part and the buckle driven part 7-1, the meshing part can be effectively clamped, so that the first stop part and the second stop part are matched, the excessive rotation of the buckle driving part and the buckle driven part 7-1 is effectively prevented, and the first clamping part and the second clamping part are enabled to be used for transitionally pressing the chip, so that the chip is damaged. In practical application, the meshing mode has a variety, adopts the sawtooth of a plurality of crisscross settings to mesh, is convenient for master pivoted distance, effectively prevents excessively to rotate the influence and the damage that cause the chip.
Referring to fig. 5 and 6, which are schematic diagrams of a three-dimensional structure and an explosion structure of a detection chip for nucleic acid detection according to an embodiment of the present invention, the detection chip for nucleic acid detection according to the embodiment of the present invention includes an uppermost sample adding layer 3, a gasket 2 disposed on a lower side of the sample adding layer 3, a pipeline layer 101 disposed on a lower side of the gasket 2, and a lowermost sealing film 104, wherein a sample adding hole 302 is disposed on an upper side of the sample adding layer 3 for adding a sample into the chip, and the sample injected into the chip undergoes a reaction of nucleic acid extraction, purification, and amplification. The sample adding layer 3 and the pipeline layer 101 of the present embodiment are movably connected to the limiting frame 106 disposed at the side of the pipeline layer 101 through the clamping strip 304, correspondingly, the inner side of the limiting frame 106 is provided with the first clamping groove 107, and the first clamping groove 107 is connected to each other through the clamping strip 304 in a matching manner, so as to realize the switching and fixing of the relative positions of the sample adding layer 3 and the pipeline layer 101. The switching of relative position indicates the change of the relative distance of application of sample layer 3 and pipeline layer 101 exactly, application of sample layer 3 is by the process of first draw-in groove 107 switching second draw-in groove for the distance between application of sample layer 3 and pipeline layer 101 has become nearly, take out gasket 2 after, application of sample layer 3 and pipeline layer 101 communicate, specifically speaking, gasket 2's primary function is protection application of sample layer 3 and pipeline layer 101 do not communicate, take out the gasket during the use again, wherein, seal membrane 104 is pasted in the downside of pipeline layer 101, in order to realize sealed. The assembled sample adding layer 3, the gasket 2, the pipeline layer 101 and the sealing film 104 form a completely closed whole, and viruses in the sample cannot leak. As shown in fig. 7, in the embodiment, a second engaging groove is further disposed on a side surface of the limiting frame 106 at the lower side of the first engaging groove 107, the second engaging groove is located at the lower side of the first engaging groove 107, when the sample adding layer 3 is transported or stored, the first card slot 107 is connected with the sample adding layer 3, when the reagent reaction is carried out, the gasket 3 is drawn out, the sample adding layer 3 is pressed downwards, so that the sample adding layer 3 is connected with the second card slot, at the same time, the pricking pin disposed on the pipeline layer 101 punctures the reagent disposed in the sample addition layer 3, so that the reagent and the sample can be mixed and reacted, and the puncture needle is arranged on the pipeline layer 101, and in the practical application process, the pipeline layer 101 is provided with an upright post, the puncture needle is arranged at the circle center of the upright post, the upper end surface of the upright post is oval, and the end surface of the upright post is inclined, so that the puncture needle on the upright post can be conveniently matched with the tail end of the reagent tube, the reagent tube can be smoothly punctured, and the reagent can be filled. According to the detection chip for nucleic acid detection provided by the embodiment of the invention, the first clamping groove 107 and the second clamping groove are arranged, so that the sample adding layer 3 and the pipeline layer 101 can be pressed to generate relative position change after the gasket 2 is extracted, and meanwhile, the pricker punctures a reagent in the sample adding layer 3 to realize sample adding and make a sample and the reagent perform a series of reactions.
In order to monitor the real-time state of the sample adding layer 3, a second strain gauge (not shown in the figure) is arranged in the first card slot 107 and the second card slot, and the second strain gauge is used for testing the stress change of each part when the first card slot 107 or the second card slot is connected with the sample adding layer 3. In the practical application process, M points are transversely taken in the first card slot 107, wherein the M points are arranged at intervals, the state of the sample adding layer 3 is judged by detecting the stress change of the M points, when the sample adding layer 3 is stored or transported, the sample adding layer 3 is connected with the first card slot 107, the first stress function in the first card slot 107 is F (F1, F2 … … fm), wherein F1 and F2 … … fm represent the stress condition of each point, and the stress conditions of all the points are approximately the same because the sample adding layer 3 is uniformly stressed; at this time, stress detection is also performed at a corresponding position in the second card slot, and since the second card slot is not connected with the sample adding layer 3, a second stress function in the second card slot is F ' (F1 ', F2 ', … … fm '), wherein F1 ', F2 ', … … fm ' are all 0; and when the sample adding layer 3 is connected with the second card slot, F1 and F2 … … fm in the first stress function F (F1 and F2 … … fm) in the first card slot are both 0, so that the current position of the sample adding layer 3 can be judged according to the first stress function and the second stress function, the problem that the experimenter leaves in the midway and cannot continuously complete subsequent experiments is solved, and other experimenters can continuously complete the subsequent experiments according to the experiment progress of the previous experimenter.
Furthermore, when the stress values at the positions in the second stress function are all 0, that is, the sample adding layer 3 is connected to the first card slot 107, a first stress function F (F1, F2 … … fm) is obtained, the stress value F1 at the first position in the first stress function is compared with the stress value fm at the mth position in the first stress function, a first positive difference value is obtained, if the first positive difference value is lower than a first preset difference value, it indicates that the sample adding layer 3 is uniformly stressed in the first card slot 107, and then the subsequent operations of pulling out the gasket 2 to press and the like can be performed; if the first positive difference is higher than the first predetermined difference, the difference between the stress value at the first position and the stress value at the mth position is larger, and it may be that the clip strip 304 of the sample-adding layer 3 or the first clip groove 107 on the limiting frame 106 has a defect or has impurities, etc., and the sample-adding layer 3 needs to be checked and adjusted, so that the first positive difference is lower than the first predetermined difference. And when the first positive difference value is lower than the first preset difference value, the back gasket 2 is output, the sample adding layer 3 is pressed, so that the sample adding layer 3 enters the second card slot under the action of external force, when the sample adding layer 3 is connected with the second card slot, a second stress function F ' (F1 ', F2 ', … … fm ') is obtained, the stress value F1 ' of the first position in the second card slot and the stress value fm ' of the M position are compared, a second positive difference value is obtained, and if the second positive difference value is lower than the second preset difference value F0 ', the situation that the sample adding layer 3 is uniformly stressed after being pressed to the second card slot is shown, and no matter whether the card strip 304 or the second card slot has no obvious abnormality. If the second positive difference is higher than the second preset difference f 0', the sample addition layer 3 needs to be adjusted, which can be referred to the adjustment when the stress value in the first slot 107 is abnormal, and is not described again.
In the practical application process, the stress response difference value between the first position and the mth position can be selected, and the positive difference value comparison can be performed on the positions of any two other points, so that the positions of all points in the first clamping groove 101 and the second clamping groove can be analyzed and checked, and the accuracy of the detection result can be further ensured.
When the sample adding layer 3 is connected with the first card slot 107, the stress difference value of the first position and the Mth position in the first stress function is detected, so that the state of the sample adding layer 3 in the first card slot 107 is checked and judged, the uniform stress of the sample adding layer in the first card slot 107 is ensured, and the states of all parts of the detection chip are in normal states; correspondingly, when the sample adding layer 3 is connected with the second clamping groove, the stress difference value of the first position and the Mth position in the second stress function is detected, so that the state of the sample adding layer 3 in the second clamping groove is checked and judged, the stress of the sample adding layer 3 in the second clamping groove is uniform, and the states of all parts of the detection chip are in normal states. In the practical application process, the sample adding layer 3 is physically pressed in the process of being pressed from the first card slot 107 to the second card slot, and abrasion to each structural component of the detection chip easily occurs in the process, so that the first card slot 107, the second card slot, the card strip 304 and other related components need to be checked to ensure the accuracy of the mixed reaction result of the sample and the reagent.
Further, when the sample adding layer 3 is connected to the second card slot, a second stress function F '(F1', F2 ', … … fm') is obtained, and then absolute values of stress difference values at positions corresponding to one another in the first stress function F (F1, F2 … … fm) and the second stress function F '(F1', F2 ', … … fm') are compared, where the first stress function F (F1, F2 … … fm) is a first stress function generated when the sample adding layer 3 and the first card slot 107 are connected, and whether the absolute value of each stress difference value is smaller than a preset standard error F0 is determined, that is, the absolute values of standard errors of | 1 '-F1 |, | F2' -F2 |, | F3 '-F3 |, | F4' -F4 |, … … and | fm '-fm | and F | 0 |, and | F | and F | 1' -F | 4974 | F685 4 '-685F 4974 | F685 3| F4' -685F If | F1' -F1 | is greater than the standard error F0 in the actual comparison process, the card strip of the sample addition layer 3 or the card slot of the pipeline layer needs to be detected at the first position, and the comparison methods from other second positions to the mth position are similar, and if the absolute value of the stress difference at a certain position is greater than the standard error F0, the reason of the data abnormality needs to be searched until the relevant abnormality is eliminated, and the detection is performed again.
In the practical application process, in order to determine whether the detection chip has an abnormality according to the stress values at the respective positions, rough estimation may be performed, for example, absolute values of stress differences at the M positions are obtained, and if the absolute values of the stress differences at more than half of the M positions are smaller than the standard difference F0, that is, if the stress at most of the M positions is uniform, the subsequent operations may be performed; of course, the user can also select the judgment criteria to be M/2 positions or 4M/5 positions or the number of other position points according to actual needs, which are not listed here.
In practical application process, probably because the pressing power degree is inhomogeneous for some of application of sample layer 3 has pressed the second draw-in groove, and some has remained in first draw-in groove or because press and lead to application of sample layer 3 to incline, detect f1 ' and fm ' in the second draw-in groove this moment, the stress of first two positions at the end must be different very greatly, not in presetting the difference within range, just need readjust application of sample layer 3 ' this moment. In practical application, the stress difference of any two points in the M points can be detected, so that the position of the sample adding layer 3 is ensured to be accurate and stable. In addition, the stress value of the first position in the first card slot 107 and the stress value of the first position in the second card slot can be detected, when the stress on the sample adding layer 3 is not uniform, f1 and f 1' have slight difference, and it can be understood that, besides the stress difference of the first position, the stress difference of other M-1 positions can be detected, which is not described one by one here. In this embodiment, the second strain gauge provides multiple modes for detecting the stress uniformity of the sample adding layer 3 in the detection chip, thereby ensuring the accuracy of subsequent nucleic acid detection.
In the practical application process, possible factors causing slight changes of stress values of the second strain gauges in the first clamping groove 107 and the second clamping groove include abrasion of the sample adding layer 3, inclination, external impurities, abrasion of the second strain gauge and the like, and the examination needs to be carried out according to data of the first stress function and the second stress function to ensure that the sample adding layer 3 and the pipeline layer 101 are stably butted to ensure that subsequent fluid enters a preset liquid injection port and a preset pipeline.
Particularly, through setting up the second foil gage, make in the pressfitting process, can assess the steady of pressfitting in-process, so that confirm the unstable factor in the pressfitting process, and after the block, through first foil gage, through the stress variation of first foil gage, make in the experimentation, add the sample and extract the purification and amplify the reaction process, carry out real-time supervision to the block condition of the two, prevent in the reaction process because stress variation leads to the problem of leakproofness, influence the experiment progress, through the stress variation who sets up first foil gage, can in time discover abnormal conditions, in time adjust. The sealing condition of the sample adding layer and the pipeline layer can affect the efficiency of the amplification reaction, so that the arrangement of the first strain gauge is necessary.
Specifically, the clamping degree of the first clamping part and the second clamping part is determined according to the stress detected by the first strain gauge.
Particularly, in the practical application process, the extrusion force on application of sample layer and pipeline layer is shown to the stress size of first foil gage, if the extrusion force on application of sample layer and pipeline layer is too big, then correspondingly the chucking degree of first clamping part and second clamping part is low once, be convenient for adjust application of sample layer and pipeline layer, and if the extrusion force on application of sample layer and pipeline layer also be exactly the stress of first foil gage less, it is better to show application of sample layer and pipeline layer block, consequently can be with first clamping part and the higher of second clamping part chucking degree in the use, prevent that the chip pine from taking off.
As shown in fig. 6, the lower side of the gasket 2 according to the embodiment of the present invention is further provided with a slide rail 202, and correspondingly, the upper side of the pipeline layer 101 is provided with a slide groove 108, and the slide rail 202 is connected with the slide groove 108 in a matching manner, so as to realize the sliding connection between the gasket 2 and the pipeline layer 101. The slide groove 108 of the present embodiment is provided inside the stopper 106 on the pipe layer 101. The end of the gasket 2 is provided with a plurality of notches and protrusions arranged at intervals, wherein the slide rail 202 is arranged on the bottom surface of the outermost protrusion.
As shown in fig. 6, the sample application hole 302 of the present embodiment is provided with a sample application hole cover 303 for sealing. Still set up buckle structure at application of sample layer 3 and pipeline layer 101, be provided with first buckle 301 in one side of application of sample layer 3, the downside of first buckle 301 stretches out the end and stretches out application of sample layer 3's bottom is installing application of sample layer 3 and pipeline layer 101 cooperation back together, through first buckle 301 joint on the side of pipeline layer 101 to prevent application of sample layer 3 and pipeline layer 101 separation.
As shown in fig. 6, the pipeline layer 101 of the present embodiment is provided with two first single valves 102 for controlling the stopping or flowing of the liquid in the pipeline layer 101 during the reaction process; the pipeline layer 101 is further provided with a double valve 103 for cutting off a road for fluid in the pipeline or allowing the fluid to pass through, the double valve 103 is communicated with the amplification bin through the pipeline, and the double valve 103 is used for controlling two ends of the amplification bin to be closed or opened simultaneously so that a closed cavity is formed inside the amplification bin. In fig. 2, handles 201 are further disposed on two sides of the gasket 2, so as to facilitate extraction of the detection chip for nucleic acid detection. In the embodiment of the invention, the amplification bin is arranged at the edge of the pipeline layer 101, and the amplification bin is of a semi-elliptical structure, so that not only can the reaction of the reaction reagent be realized, but also the convenient positioning and installation can be realized through the convex semi-elliptical structure when in use.
Continuing to refer to fig. 6, in the present embodiment, a row of pricker 105 is disposed on the pipeline layer 101, after the sample-adding layer 3 and the gasket 2 are clamped together, the handle 201 is operated to slide the gasket 2 along the sliding groove 108, when the gasket 2 is pulled out when the gasket slides to a position where the gasket cannot advance, the sample-adding layer 3 is pressed into the second clamping groove from the first clamping groove 107, so that the pricker 105 can prick a reagent tube in the sample-adding layer 3, and further the pricker 105 is communicated with a reagent in the sample-adding layer 3, and when a fluorescence sequence marked in the reagent is complementarily matched with a nucleic acid pricker at a corresponding position, a group of probe sequences with completely complementary sequences is obtained by determining a probe position with the strongest fluorescence intensity. A baffle is disposed outside the needle 105, and it acts as a stop and a positioning function when the sample injection layer 3 is matched with the pipeline layer 101.
Specifically, in the embodiment of the present invention, in the sample loading state, a plurality of sets of reagent tubes are disposed in the sample loading layer 3, the sample loading layer 3 is clamped with the first clamping groove 107 through the clamping strip 304 thereon, in the initial installation state, the sample loading layer 3 is matched with the pipeline layer 101 from top to bottom, and the reagent in the reagent tubes is isolated from the lancet through the gasket 2, so that the lancet and the reagent are prevented from being mixed due to vibration in the transportation process, the sample loading layer and the pipeline layer are protected from being disconnected, and puncturing is avoided. When a test is needed, the gasket 2 is outwards pulled out along the sliding groove 108, and after the gasket 2 is outwards pulled out along the sliding groove 108, the sample adding layer 3 is downwards pressed, so that the clamping strip 304 on the sample adding layer 3 is clamped with the second clamping groove, at the moment, the pricking pin 105 arranged on the pipeline layer 101 is mixed with a reagent of the sample adding layer 3, and the reagent is introduced into the pipeline layer 101 for testing.
Specifically, the gasket structure is arranged, so that the detection chip for detecting nucleic acid can be stored completely in the process of storing and transporting the reagent, and when the detection chip is used, the reagent can be introduced into the pipeline layer only by drawing out the gasket and pressing the sample adding layer downwards.
FIG. 7 is a schematic view of a sample-adding layer according to an embodiment of the present invention; in this embodiment, a sample loading chamber is located below the sample loading hole 302, the sample loading chamber can be connected to a reagent tube for loading a reagent or a sample, a reagent outlet 312 is located at the lower portion of the sample loading chamber, a sealing structure 313 is located between the reagent outlet 312 and the sample loading chamber for sealing, and when a reagent needs to be loaded, the spike 105 can puncture 313 to allow the reagent to enter the fluid pipeline along the reagent outlet 312. A pressurizing structure is arranged on one side of the sample adding bin and comprises a tube wall 305, a piston 308 is arranged in the tube wall 305, and the piston 308 moves towards the sample adding bin to push the reagent in the sample adding bin to flow out to a reagent outlet 312; of course, when the reagent is required to be withdrawn, the piston 308 may also withdraw the reagent or other waste liquid, and a sealing ring 311 is provided at the end of the piston rod of the piston 308 for sealing.
Continuing to refer to fig. 7, the piston rod of this embodiment is further provided with a nut 307, which is in threaded connection with the nut 307 to realize relative rotation motion, and correspondingly, an output structure, such as an air cylinder and an oil cylinder, is provided at one end of the piston rod, or the output structure is connected to the piston rod by rotating, such as a motor and a lead screw, at this time, the piston rod makes rotation motion, and only needs to push the reagent to flow out to the reagent outlet. Correspondingly, a guide sleeve 306 is sleeved outside the nut 307, and a corresponding shaft shoulder is arranged inside the pipe wall 305 to position and fix the guide sleeve 306; snap rings 314 are further provided at the outer sides of both ends of the guide sleeve 306 to catch the corresponding guide sleeve 306. A sheath 309 is also provided outside the guide sleeve 306 to protect the piston rod, the nut 307 and the guide sleeve 306. When reagent is injected into the pipeline layer 101, the piston moves toward the sample loading bin to increase the pressure therein, so as to push the reagent to flow toward the reagent outlet 312, thereby realizing reagent injection. With reference to fig. 6, in the embodiment of the present invention, a plurality of groups of reagent tubes are provided, and in the embodiment, five groups of reagent tubes are provided, and different or the same reagents are sequentially applied to the pipeline layer according to experiment requirements, so that the use efficiency can be greatly improved.
As shown in fig. 7, a second buckle 310 is disposed below the sample adding layer 3, and the second buckle 310 is disposed on a side opposite to the first buckle 301 to prevent the sample adding layer 3 from sliding.
It can be seen that this embodiment is integrated at chip pipeline layer to complicated experimentation, can control the liquid trend to can improve work efficiency effectively.
Referring to fig. 8, as shown in fig. 8, it should be noted in advance that, in fig. 6, the first single valve 102 and the double valve 103 are both disposed on the pipeline layer 101, and in fig. 8, the first single valve and the double valve are also included, and different reference numerals are used, namely, the first single valve 210 and the first double valve 211, and the first portion 271 and the second portion 281 of the double valve constitute the double valve 103.
As can be understood by those skilled in the art, the tube structure disposed on the tube layer is unable to perform related nucleic acid detection tests when the sample adding layer is not connected to the tube layer, so that it is necessary to perform nucleic acid extraction, purification and amplification reactions when the tube layer is in contact with the sample adding layer.
Specifically, as shown in fig. 8, the pipeline layer includes a first sample inlet 21, a first reagent inlet 22, a second reagent inlet 23, a third reagent inlet 24, a fourth reagent inlet 25, a purification chamber 26 and an amplification chamber 27, the first sample inlet 21 and the first reagent inlet 22 are connected through a first pipeline, a first single valve 211 is disposed on the first pipeline, the purification chamber 26 includes an inlet 261 and an outlet 262, the first sample inlet 21 and the inlet 261 are connected through a second pipeline 16, the first reagent inlet 22 and the outlet 262 are connected through a third pipeline 126, and the second reagent inlet 23, the third reagent inlet 24 and the fourth reagent inlet 25 are all connected with the inlet 262 through a fourth pipeline 610; the first end of the amplification chamber 27 is provided with a first double-valve portion 271, the second end of the amplification chamber is provided with a second double-valve portion 281, the first double-valve portion 271 is connected with the other single valve 212 through a fifth pipeline, the other single valve 212 is connected with the outlet 262 through a sixth pipeline 126, and the first double-valve portion 271 and the second double-valve portion 281 are opened and closed simultaneously.
Referring to fig. 8, a first sample inlet 21, a first reagent inlet 22, a second reagent inlet 23, a third reagent inlet 24, a fourth reagent inlet 25, a purification bin 26 and a PCR amplification bin 27 are further described, the first sample inlet 21 and the first reagent inlet 22 are connected through a first pipeline, a second single valve 211 is disposed on the first pipeline, the purification bin 26 includes an inlet 261 and an outlet 262, the first sample inlet 21 and the inlet 261 are connected through a second pipeline 16, the first reagent inlet 22 and the outlet 262 are sequentially connected through a seventh pipeline 92, a first buffer bin 29, a first single valve 212 and a third pipeline 126, and the second reagent inlet 23, the third reagent inlet 24 and the fourth reagent inlet 25 are all connected with the inlet 261 through a fourth pipeline 610; the first end of the PCR amplification chamber 27 is connected to the first part 271 of the double valve, the second end of the PCR amplification chamber is connected to the second part 281 of the double valve, the second part 281 of the double valve is connected to the outlet 262 of the purification chamber 26 through the eighth pipeline 86, the first part 271 of the double valve is connected to the first reagent port 22 through the second buffer chamber 210, the buffer chamber pipeline 97 and the first buffer chamber 29, and the first part 271 of the double valve and the second part 281 of the double valve are operated in a manner of being simultaneously closed or simultaneously opened.
Specifically, the purification and amplification device provided by the embodiment of the present invention further includes a first buffer bin 29, the first buffer bin 29 may also be referred to as a waste liquid bin, a high water absorption sponge is disposed in the first buffer bin, and mainly functions that after the lysate in the first reagent port 22 is injected, a small amount of liquid will remain in the lysate bin, the first reagent port 22 is used as a driven bin matched with other reagents and enters the purification bin, in this process, the piston structure connected to the first reagent port 22 will perform a suction motion, in order to avoid mixing a small amount of overflowing waste liquid into the whole liquid path system, the first buffer bin is disposed to absorb a small amount of waste liquid, one end of the first buffer bin 29 is connected to the first reagent port 22 through a seventh pipeline 92, the other end of the first buffer bin 29 is connected to a fifth pipeline to form a three-way pipeline, a second buffer bin 210 is further disposed between the three-way pipeline and the PCR amplification bin 27, be provided with the sponge in the second surge bin 210 for strengthen protection PCR and amplify storehouse 27, the second portion 281 of bivalve through eighth pipeline 86 with outlet 262 connects, be provided with second single valve 211 on the first pipeline, the one end of second single valve 211 pass through ninth pipeline 111 with first introduction port 21 connects, the other end of second single valve 211 pass through tenth pipeline 112 with first reagent mouth 22 connects.
When the amplification reaction is performed, firstly, injecting a sample, which may be blood or a swab, into the first sample inlet 21, injecting a first reagent, which is a lysate, into the first reagent inlet 22, opening the second single valve 211 to mix the sample and the first reagent via the first pipeline, so as to obtain a first product, which includes a liquid, the liquid enters the purification chamber via the second pipeline 16, the purification chamber 26 contains magnetic beads, the sample is decomposed by the lysate, nucleic acid substances and proteins are separated, the purification chamber is a reaction chamber for nucleic acid extraction and purification, the extraction is a reaction chamber for extracting nucleic acid substances from a mixture of nucleic acid substances and proteins, the purification is a step of cleaning the extracted nucleic acid substances, then closing the second single valve, opening the first single valve 212, and injecting a second reagent into the second reagent inlet 23, the second reagent is a cleaning solution, the second reagent enters the purification chamber through the fourth pipeline 610 and reacts with the liquid to obtain a second product, the third reagent is injected into the third reagent port 24, the third reagent is a cleaning solution, the third reagent enters the purification chamber through the fourth pipeline 610 and reacts with the second product to obtain a third product, the fourth reagent is injected into the fourth reagent port 25 and is an eluent, so that the nucleic acid substance is separated from the magnetic beads, the fourth reagent enters the purification chamber through the fourth pipeline 610 and reacts with the third product to obtain a fourth product, and the fourth product is introduced into the PCR amplification chamber 27 through the sixth pipeline 126, the first check valve 212 and the fifth pipeline to perform an amplification reaction.
Adding a sample into the first sample port 21, closing the first single valve 212, the first part 271 of the double valve and the second part 281 of the double valve, opening the second single valve 211, and then pushing a first reagent into the first reagent port 22, wherein the sample may be blood, nasopharyngeal swab, sample along the ninth pipeline 111, and the first reagent along the tenth pipeline 112, mixing in the first pipeline, in order to make the mixing of the sample and the first reagent more sufficient, in the practical application process, a push-suction device, specifically a piston structure, may be added at the first reagent port and the first sample port, to accelerate the micro-flow of the sample or reagent in the ninth pipeline 111 and the tenth pipeline 112, and to fully mix and react, so as to obtain a first biological product in the first pipeline, and the first biological product fills the first pipeline.
The second single valve 211 is closed, the first portion 271 of the double valve and the second portion 281 of the double valve are closed, and the first single valve is opened. At this time, the first product is respectively left in the ninth pipeline 111 and the tenth pipeline 112, the first product comprises liquid and gas, the liquid enters the purifying bin 26 from the first sample inlet 21 through the second pipeline 16, the liquid fills the purifying bin 26 and overflows in the sixth pipeline 126, meanwhile, under the buffering action of the second buffer bin 29, the gas of the first product enters the sixth pipeline 126 through the seventh pipeline 92 and the first single valve 212, and the liquid and the gas are merged in the sixth pipeline 126. When the second reagent is added, the second reagent is pushed into the second reagent port 23, the second reagent actually enters the purification bin 26 from the inlet 261 along the fourth pipeline 610, magnetic beads are arranged in the purification bin 26 so that the second reagent enters the purification bin 26 filled with the liquid, the magnetic beads in the purification bin 26 are scattered under the action of ultrasonic waves, nucleic acid in the liquid in the purification bin is sufficiently contacted and adsorbed by the magnetic beads, the first reagent port 22 is sucked into the first reagent port while the second reagent is pushed, so that the second reagent smoothly enters the purification bin 26, the second reagent reacts with the first product to obtain a second product, and the purification bin 26 is filled with the second product. Some waste liquid may be generated while the second product is being produced, and the waste liquid may be discharged to the first reagent port 22 and/or the first sample port 21, and optionally, the waste liquid may be discharged to the first reagent port 22 through 126, or the waste liquid may be discharged to the first sample port 21 through 16.
When the third reagent and the second product are mixed, the second reagent and the third reagent clean the liquid in the purification bin 26 to realize the extraction and purification of nucleic acid, when the third reagent port 24 pushes the third reagent through the fourth pipeline 610, the second reagent port 23 sucks part of the second product, then when the second reagent port 23 pushes the second product, the third reagent port 24 sucks the third reagent, and finally, the waste liquid of the reaction is sucked into the second reagent port 23 and/or the first reagent port 22 and/or the first sample port 21; when the fourth reagent and the third product are mixed, and the fourth reagent port 25 is pushed in the fourth reagent, the third reagent port sucks a part of the third product, and then when the third reagent port 24 is pushed in the third product, the fourth reagent port 25 sucks the fourth reagent, the fourth reagent is an eluent, and when the purification cartridge is filled with the fourth product after the reaction of the third reagent and the third reactant, the first single valve 212 and the second single valve 211 are closed, the first part 271 of the double valve and the second part 281 of the double valve are opened, and the fourth product fills the PCR amplification cartridge 26 from the outlet 262 along the eighth pipeline 86, and further the first part 271 of the double valve and the second part 281 of the double valve are closed. Compared with the previous embodiment of the present invention, the fourth product is introduced into the PCR amplification chamber 27 through the eighth pipeline 86, and compared with the PCR amplification chamber 27 through the sixth pipeline 126 and the fifth pipeline, the eighth pipeline 86 is not contaminated by other liquid or gas during the reaction process, and is clean, so that the purity of the substance entering the PCR amplification chamber 27 can be ensured.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention; various modifications and alterations to this invention will become apparent to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. A buckle device, comprising:
the bin gate buckle, the transmission mechanism and the key are arranged on the inner side of the fixing frame, one end of the transmission mechanism is provided with a long hole, and the key is connected with the long hole of the transmission mechanism through a connecting shaft;
The bin gate buckle comprises a buckle driving piece and a buckle driven piece, the buckle driving piece and the buckle driven piece are arranged oppositely, the buckle driving piece comprises a first meshing part, a first clamping part and a connecting tongue, the connecting tongue is connected with the transmission mechanism, the buckle driven piece comprises a second meshing part, a second clamping part and a first torsion spring, the buckle driven piece is connected with the fixing frame through the first torsion spring, the first meshing part is meshed with the second meshing part, the first clamping part is matched with the second clamping part to clamp the upper cover of the chip, and a detection chip is arranged in a clamping seat on the upper cover of the chip;
the detection chip comprises a sample adding layer arranged at the uppermost end, a gasket arranged at the lower side of the sample adding layer and a pipeline layer arranged at the lower side of the gasket;
wherein,
the upper side of the sample adding layer is provided with a sample adding hole for adding a sample into the chip, and the sample injected into the chip is subjected to nucleic acid extraction, purification and amplification reaction in sequence;
the sample adding layer and the pipeline layer are movably connected with a limiting frame arranged on the side part of the pipeline layer through a clamping strip, correspondingly, a first clamping groove is arranged on the inner side of the limiting frame and is mutually matched and connected through the clamping strip, so that the relative position switching and fixing of the sample adding layer and the pipeline layer are realized;
The second clamping groove is arranged on the inner side of the limiting frame and is positioned below the first clamping groove;
when in transportation or storage, the sample adding layer is connected with the first clamping groove;
when the sample injection device is used, the gasket is pulled out, the sample injection layer is pressed downwards to be connected with the second clamping groove, and meanwhile, the puncture needle is arranged on the upright column on the pipeline layer and is used for puncturing the reagent arranged in the sample injection layer so as to enable the reagent and the sample to be mixed and reacted;
after the sample adding layer and the pipeline layer are pressed, a first strain gauge arranged at the bottom of the sample adding layer detects the extrusion force between the sample adding layer and the pipeline layer so as to determine the stress uniformity of the sample adding layer and the pipeline layer in the pressing process;
when horizontal acting force is applied to the key, the connecting shaft of the key moves in the elongated hole to drive the transmission mechanism to rotate, the transmission mechanism drives the connecting tongue, and the connecting tongue enables the first engaging part to be engaged reversely so as to realize the separation of the first clamping part and the second clamping part;
and determining the clamping degree of the first clamping part and the second clamping part according to the stress detected by the first strain gauge.
2. The buckle device according to claim 1, wherein the transmission mechanism comprises a first connecting rod and a second connecting rod, the first connecting rod comprises a first long rod, a first short rod and a first snap spring, the first long rod is rotatably connected with the first short rod, a first transition step surface is arranged between the first long rod and the first short rod, the first snap spring is welded and fixed with the first long rod, the first short rod is provided with the strip hole, the second connecting rod comprises a second long rod, a second short rod, a second snap spring and a second torsion spring, the second long rod is rotatably connected with the second short rod, a second transition step surface is arranged between the second long rod and the second short rod, the second snap spring is welded and fixed with the second long rod, the first long rod is provided with a strip hole, the first long rod is connected with the second short rod, and one end of the second torsion spring is fixed on the fixing frame, the other end of the second torsion spring is connected with the second long rod.
3. The buckle device according to claim 2, further comprising a compression spring, wherein the button comprises a button cap and a compression bar, the compression spring is disposed on a lower side of the compression bar for transferring an acting force, the compression spring is disposed on a lower side of the compression bar for reducing a contact acting force with the chip bin, the connection tongue comprises a straight arm and a protrusion, the straight arm and the protrusion are integrally formed, the second long rod is also provided with a protrusion, the protrusion slides along a curvature of the protrusion during the opening process of the first clamping portion and the second clamping portion, the bottom ends of the first engagement portion and the second engagement portion are flat, and the first engagement portion and the second engagement portion each comprise a plurality of staggered saw teeth.
4. The buckle device of claim 3, wherein the buckle driver further comprises a first backstop disposed between the connection tongue and the first engagement portion to constrain travel of the buckle driver during movement of the buckle driver;
the snap follower further comprises a second backstop for constraining travel of the snap follower during movement of the snap follower.
5. The buckle device according to claim 4, wherein two protrusions are disposed on the fixing frame, the first clamping portion and the second clamping portion are disposed in the protrusions when the fixing frame is opened, and when the first clamping portion and the second clamping portion are closed, the bending portions of the first clamping portion and the second clamping portion extend out of the protrusions and contact with the detection chip.
6. The buckle device according to claim 1,
the detection chip further comprises a sealing film and a second strain gauge, the sealing film is arranged on the lower side of the pipeline layer and used for realizing sealing, and the second strain gauge is respectively arranged in the first clamping groove and the second clamping groove; taking M positions in the first card slot transversely, detecting the stress at the M positions by the second strain gauge, and marking as a first stress function F (F1, F2 … … fm), wherein the selected positions in the second card slot correspond to the positions in the first card slot one by one, and the second stress function of the second card slot is F '(F1', F2 ', … … fm'), and judging the position of the sample adding layer according to the first stress function and the second stress function;
When the sample adding layer is connected with the first clamping groove, firstly, f1 and fm are compared to obtain a first positive difference value, and if the first positive difference value is higher than a first preset difference value f0, the sample adding layer is readjusted; if the first positive difference is lower than a first preset difference f0, performing subsequent operation;
when the sample adding layer is connected with the second clamping groove, f1 ' and fm ' are compared to obtain a second positive difference value, and if the second positive difference value is higher than a second preset difference value f0 ', the sample adding layer is readjusted; if the second positive difference is lower than a second predetermined difference f 0', the subsequent operation is performed.
7. A buckle device according to claim 6,
when the sample adding layer is connected with the second card slot, comparing absolute values of stress difference values at positions corresponding to one another in a first stress function F (F1, F2 … … fm) and a second stress function F '(F1', F2 ', … … fm'), wherein the first stress function F (F1, F2 … … fm) is a function generated when the sample adding layer is connected with the first card slot, judging whether the absolute value of each stress difference value is smaller than a preset standard error F0, if so, continuing to operate, and if not, determining a corresponding position of the absolute value of a certain group of corresponding stress difference values so as to determine the damage of the sample adding layer or the pipeline layer.
8. A buckle device according to claim 7,
the lower side of the gasket is also provided with a slide rail, correspondingly, the upper side surface of the pipeline layer is provided with a slide groove, and the slide rail is connected with the slide groove in a matching way so as to realize the sliding connection between the gasket and the pipeline layer;
the sliding groove is arranged on the inner side of the limiting frame on the pipeline layer;
the end part of the gasket is provided with a plurality of notches and bulges which are arranged at intervals, wherein the slide rail is arranged on the bottom surface of the bulge at the outermost side.
9. The buckle device according to any one of claims 1 to 8, wherein a plurality of sample loading chambers are arranged at intervals below the sample loading hole, a reagent outlet is arranged at an end of each sample loading chamber, and a sealing structure is arranged between the reagent outlet and the sample loading chamber for sealing;
a pressurizing structure is arranged on one side of the sample adding bin and comprises a tube wall, a piston is arranged in the tube wall, and the piston moves back and forth along the tube wall to push the reagent in the piston to flow out or be drawn back to a reagent outlet; and a sealing ring is arranged at the end part of the piston rod of the piston and used for sealing.
10. The buckle device according to claim 9, wherein the piston rod is further provided with a nut, the nut is in threaded connection with the nut, a guide sleeve is sleeved outside the nut, a corresponding shaft shoulder is arranged inside the pipe wall and used for positioning and fixing the guide sleeve, and snap rings are further arranged outside two ends of the guide sleeve and used for clamping the corresponding guide sleeve; and a sheath is arranged on the outer side of the guide sleeve and used for protecting the piston rod, the screw cap and the guide sleeve.
CN202110130185.8A 2021-01-29 2021-01-29 Fastening device Active CN113175466B (en)

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CN104534235A (en) * 2014-12-26 2015-04-22 青岛歌尔声学科技有限公司 Double-buckle device and wearing product structure
JP2015531690A (en) * 2012-08-07 2015-11-05 Smc株式会社 Clamping device
CN108799263A (en) * 2018-06-20 2018-11-13 杭州戬威机电科技有限公司 A kind of ultrasound stress mornitoring analyzer
CN108865821A (en) * 2018-07-09 2018-11-23 杭州霆科生物科技有限公司 A kind of the nucleic acid isothermal amplification chip and application method of integrated thermal cracking
CN111250183A (en) * 2020-02-17 2020-06-09 北京中科生仪科技有限公司 Liquid injection pump driving device for micro-fluidic system
CN212330756U (en) * 2019-12-17 2021-01-12 深圳市博辉特科技有限公司 Double-buckle structure

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JP4936235B2 (en) * 2009-12-21 2012-05-23 株式会社日立プラントテクノロジー REAGENT OPENING MECHANISM OF LIGHT EMITTING DEVICE AND OPENING NEEDLE CONTROL METHOD IN REAGENT OPENING MECHANISM

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015531690A (en) * 2012-08-07 2015-11-05 Smc株式会社 Clamping device
CN104534235A (en) * 2014-12-26 2015-04-22 青岛歌尔声学科技有限公司 Double-buckle device and wearing product structure
CN108799263A (en) * 2018-06-20 2018-11-13 杭州戬威机电科技有限公司 A kind of ultrasound stress mornitoring analyzer
CN108865821A (en) * 2018-07-09 2018-11-23 杭州霆科生物科技有限公司 A kind of the nucleic acid isothermal amplification chip and application method of integrated thermal cracking
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