CN115747025A - Full-automatic nucleic acid extraction and PCR detect all-in-one - Google Patents
Full-automatic nucleic acid extraction and PCR detect all-in-one Download PDFInfo
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- CN115747025A CN115747025A CN202211260269.4A CN202211260269A CN115747025A CN 115747025 A CN115747025 A CN 115747025A CN 202211260269 A CN202211260269 A CN 202211260269A CN 115747025 A CN115747025 A CN 115747025A
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Abstract
The invention belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a full-automatic nucleic acid extraction and PCR detection integrated machine, which comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the nucleic acid extraction module and the PCR detection module are arranged in the machine shell; the nucleic acid extraction module comprises a consumable unit, a liquid transfer unit and a magnetic adsorption unit, wherein the consumable unit is used for storing consumables required by nucleic acid extraction and PCR reaction; the liquid transfer unit is arranged between the consumable unit and the PCR detection module and is used for picking up the tip head and sucking the reagent and the sample in the consumable unit into the tip head through the tip head; the magnetic adsorption unit is arranged below the pipetting unit and comprises a magnet, and the magnet is close to the tip head for magnetic adsorption. The invention can realize repeated adsorption of the cracking solution for many times and recover magnetic beads, so that the content of the magnetic beads in the waste liquid is controllable; the frequency of nucleic acid extraction is not limited by the structure, and the method is favorable for magnetic bead recovery and subsequent washing and amplification operations.
Description
Technical Field
The invention belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a full-automatic nucleic acid extraction and PCR detection integrated machine.
Background
Nucleic acid extraction is an essential link in various molecular biology experiments, and almost every laboratory requires nucleic acid extraction for separation and purification of biomolecules. The nucleic acid extractor is an instrument which automatically finishes nucleic acid extraction work by using a matched nucleic acid extraction reagent, and is widely applied to the fields of clinical disease diagnosis, microbiological detection and the like; after nucleic acid extraction, PCR detection, which is a molecular biological technique for amplifying specific DNA fragments, is required. The nucleic acid extraction method mainly adopts two extraction methods, namely a centrifugal column method and a magnetic bead method at present, the magnetic bead method is a novel nucleic acid extraction technology taking nanometer biological magnetic beads as carriers, nucleic acid molecules can be specifically identified and combined with silicon hydroxyl on the surfaces of the magnetic beads, and aggregation or dispersion is carried out under the action of an external magnetic field, so that the nucleic acid is separated and purified. At present, the magnetic bead method nucleic acid extraction generally comprises four main steps of cracking, combining, washing and eluting, and each step can be realized by a plurality of different methods separately or jointly.
In the prior art, a nucleic acid extraction device, such as the nucleic acid extraction and PCR detection integrated machine disclosed in chinese patent CN113388507B, includes a casing, a nucleic acid extraction module, and a PCR detection module, where the casing is a hollow casing, and the nucleic acid extraction module and the PCR detection module are disposed inside the casing. And a magnetic adsorption device for nucleic acid extraction disclosed in prior art CN215906208U, comprising an installation base plate, wherein a motor is arranged below the installation base plate, and a magnet fixing seat is arranged above the installation base plate; an output shaft of the motor is connected with a lead screw, and the lead screw penetrates through the mounting bottom plate to be connected with the magnet fixing seat; a row of grooves with equal intervals are arranged on the magnet fixing seat, and magnets are embedded in the grooves; the Hall induction magnet is arranged below the magnet fixing seat, and a Hall sensor is arranged below the mounting base plate and corresponds to the Hall induction magnet. The utility model automatically realizes the dispersion and aggregation of magnetic beads; meanwhile, the Hall sensor is used for accurately controlling the starting and stopping of the motor, so that the accurate position control of the magnet is realized.
Above-mentioned prior art's magnetism adsorbs the subassembly and all is located the kit below, through drive arrangement drive magnetism adsorbs subassembly reciprocating motion from top to bottom, is close to the lysate and realizes magnetism and adsorbs, and its step includes: adding lysis solution into the sample, blowing and uniformly mixing, adsorbing by magnetic beads, removing the lysis solution, adding washing solution to wash the magnetic beads, adsorbing by the magnetic beads, removing the washing solution, adding elution buffer solution and the like, thereby obtaining the purified nucleic acid. However, in this way, after the lysis is completed, the waste liquid needs to be removed, and the removed waste liquid is put into a waste liquid tube away from the magnet; in order to remove the waste liquid as thoroughly as possible, the pipetting tip needs to extend into the bottom of the deep-well plate, if the tip is too close to the magnetic bead, the magnetic bead and the waste liquid can be sucked together, then the magnetic bead in the waste liquid can not be removed again through magnetic adsorption, the requirement of the content of the magnetic bead in the waste liquid can not be met, and the magnetic bead is reduced, thereby influencing subsequent washing and nucleic acid amplification.
In summary, the magnetic adsorption assembly in the prior art cannot realize repeated and repeated adsorption, and is liable to affect subsequent washing and nucleic acid amplification.
Disclosure of Invention
In order to solve the technical problems that the magnetic adsorption component in the prior art cannot realize repeated and repeated adsorption and is easy to influence subsequent washing and nucleic acid amplification, the invention provides a full-automatic nucleic acid extraction and PCR detection integrated machine.
In order to realize the purpose, the technical scheme of the invention is as follows:
a full-automatic nucleic acid extraction and PCR detection integrated machine comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the nucleic acid extraction module and the PCR detection module are arranged in the machine shell, and a lifting door assembly for separation is arranged between the nucleic acid extraction module and the PCR detection module; the nucleic acid extraction module comprises a consumable unit, a liquid transfer unit and a magnetic adsorption unit, wherein the consumable unit is used for storing consumables required by nucleic acid extraction and PCR reaction; the liquid transfer unit is arranged between the consumable unit and the PCR detection module and used for picking up the tip head and sucking the reagent and the sample in the consumable unit into the tip head through the tip head; the magnetic adsorption unit is arranged below the pipetting unit and comprises a magnet, and the magnet is close to the tip head for magnetic adsorption.
Further, the liquid transfer unit comprises a support frame, the support frame is fixed on the machine shell, a tip head is arranged on one side of the support frame, and the tip head reciprocates up and down along the support frame; the lower end of the support frame is provided with the magnetic adsorption unit; the magnetic adsorption unit comprises a mounting plate, and the mounting plate is fixedly connected with the support frame.
Furthermore, the magnetic adsorption unit further comprises a linear driving device, a guide assembly and a magnet fixing plate, wherein the linear driving device is fixedly connected to the mounting plate and is used for driving the magnet fixing plate to reciprocate; the guide assembly comprises a guide shaft and a linear bearing penetrating through the guide shaft, the guide shaft penetrates through the magnet fixing plate, and the linear bearing is fixedly connected with the magnet fixing plate; the magnet is arranged on the magnet fixing plate.
Furthermore, a fixing frame is arranged below the mounting plate, folded edges bent downwards are arranged at two ends of the fixing frame, and the guide shafts are fixed on the folded edges at two ends of the fixing frame.
Furthermore, a spring is arranged on the guide shaft in a penetrating mode, one end of the spring is in contact with the linear bearing, and the other end of the spring is in contact with the folded edge far away from the magnet.
Furthermore, the linear driving device comprises a motor, a gear and a rack, the motor is arranged on the mounting plate, the output end of the motor is fixedly connected with the gear and drives the gear to rotate, the rack is meshed with the gear, and the rack is fixedly connected with the magnet fixing plate.
Furthermore, a plurality of grooves are formed in the side face, close to the tip head, of the magnet fixing plate at equal intervals, the magnets are arranged in the grooves, and the magnets correspond to the tip head one to one.
Furthermore, a first air inlet is arranged above the machine shell, a first air outlet is arranged below the rear side of the machine shell, a second air inlet is arranged above the first air outlet, and a second air outlet is arranged above the second air inlet; a first air duct is arranged between the lower part of the nucleic acid extraction module and the bottom wall of the shell, air is fed from a first air inlet through the first air duct, and air is discharged from a first air outlet through the nucleic acid extraction module;
the bottom of the shell is provided with a third air inlet, and outside air enters from the third air inlet, converges into the air path behind the first air duct and then exits from the first air outlet;
and a second air duct is arranged in the PCR detection module, air is fed from a second air inlet through the second air duct, and air is discharged from a second air outlet through the PCR detection module.
Furthermore, a first filter screen is arranged at the first air inlet, a second filter screen is arranged at the first air outlet, a third filter screen is arranged at the second air inlet, and a fourth filter screen is arranged at the second air outlet.
Further, the first filter screen is fixed on the inner wall above the casing by a frame.
Furthermore, a closing plate is arranged between the upper part of the nucleic acid extraction module and the shell, the right end of the closing plate is bent upwards and connected with the shell, and the left end of the closing plate extends downwards, so that external air enters the shell from the first air inlet and then enters the consumable unit downwards along the closing plate; a first air channel is arranged below the consumable unit, a fifth filter screen is arranged at the left end of the first air channel, and circulating air enters the first air channel from the fifth filter screen;
the right end of the first air duct is provided with an air blower, and the right side of the first air duct is sequentially provided with an air duct baffle, a fan, a cold water radiator and a second filter screen.
Furthermore, a third air inlet is formed in the machine shell below the air duct baffle, external air enters from the third air inlet, sequentially passes through the air duct baffle, the fan, the cold water radiator and the second filter screen, and is exhausted from the first air outlet.
Furthermore, a gun arranging support is arranged on one side of the support frame and is connected with the support frame in a sliding mode, a plurality of connecting pipes are arranged on the gun arranging support, and tip heads are arranged below the connecting pipes;
the liquid-transfering motor is fixed above the middle part of the support frame and drives the gun-arranging support to reciprocate up and down through the transition assembly.
Furthermore, the transition assembly comprises an adapter plate, and the pipetting motor is connected with the adapter plate through a lead screw and drives the adapter plate to move up and down; the two ends of the adapter plate are arranged on the gun arranging support through the buffer assemblies.
Furthermore, the buffering assembly comprises a fixing screw and a spring penetrating through the fixing screw, fixing holes are formed in two ends of the adapter plate, and the fixing screw penetrates through the fixing hole of the adapter plate to be connected with the gun arranging support.
Furthermore, a needle withdrawing plate is arranged below the gun arranging support, a plurality of through holes are formed in the needle withdrawing plate, and the connecting pipes are arranged in the through holes in a penetrating mode; the needle withdrawing mechanism is characterized in that a needle withdrawing motor is arranged on the supporting frame, and the needle withdrawing motor drives the needle withdrawing plate to move up and down through a lead screw.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the magnetic adsorption unit is arranged below the liquid transfer unit, after a sample, magnetic beads and the like are adsorbed into the tip head through the liquid transfer unit, the magnetic adsorption unit is driven to be close to the tip head to realize magnetic adsorption, the magnetic beads are adsorbed to one side of the tip head, then the waste liquid is discharged into a reagent tube, the residual magnetic beads are prevented from carrying out subsequent operation in the washing liquid, if the waste liquid contains the magnetic beads, the tip head can be used for absorbing the waste liquid again to carry out re-adsorption, and the magnetic beads are recovered, so that the content of the magnetic beads in the waste liquid is controllable; compared with the prior art in which only one adsorption can be carried out, the nucleic acid extraction frequency is not limited by the structure, and the magnetic bead recovery and the subsequent washing and amplification operations are facilitated.
According to the invention, the three air duct systems are arranged to ventilate the nucleic acid extraction module, the PCR detection module and the PCR water-cooling circulation system respectively, so that the reagents of the nucleic acid extraction module and the PCR detection module are prevented from being polluted by air, and the effectiveness of the experimental process is ensured; and the stable operation of the PCR water-cooling circulation system is ensured, and the stable operation of the temperature rising and reducing component in the PCR detection module is ensured. Meanwhile, an air cooling loop of the cold water radiator is overlapped with the first air channel, so that the air cooling volume and the air cooling cost are reduced.
The support frame is driven by the liquid transfer motor through the transition assembly, the liquid transfer motor does not directly drive the support frame, when a lead screw driven by the liquid transfer motor radially swings, the adapter plate inclines along with the lead screw, the spring can buffer and correct the force transmitted by the adapter plate, the influence of the radial swing of the lead screw on the support frame is reduced, the possibility of the inclination of the support frame is reduced, and the tip head can smoothly transfer liquid.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic view of the internal structure of the present invention.
Fig. 3 is a schematic structural diagram of the pipetting unit and the magnetic adsorption unit of the present invention.
Fig. 4 is a schematic structural view of the pipetting unit of the invention.
Fig. 5 is a schematic structural view of the magnetic adsorption unit according to the present invention.
FIG. 6 is a schematic view of the air duct of the present invention.
FIG. 7 is a schematic diagram of the structure of the nucleic acid extraction module according to the present invention.
Fig. 8 is an exploded view of the first air duct according to the present invention.
Fig. 9 is a schematic structural view of a transition assembly according to the present invention.
Description of the reference numerals:
1-machine shell, 2-consumable unit, 3-liquid-transferring unit, 301-supporting frame, 302-tip head, 303-gun arranging support, 304-connecting pipe, 305-liquid-transferring motor, 306-adapter plate, 307-lead screw, 308-fixing screw, 309-spring, 310-needle withdrawing plate, 311-needle withdrawing motor, 312 lead screw nut,
4-magnetic adsorption unit, 401-magnet, 402-mounting plate, 403-magnet fixing plate, 404-guide shaft, 405-linear bearing, 406-fixing frame, 407-spring, 408-motor, 409-gear, 410-rack, 411-optical coupling induction sheet, 412-optical coupling, 413-end seat,
5-a first air inlet, 6-a first air outlet, 7-a second air inlet, 8-a second air outlet, 9-a third air inlet, 10-a first filter screen, 11-a second filter screen, 12-a third filter screen, 13-a fourth filter screen, 14-a frame, 15-a sealing plate, 16-a fifth filter screen, 17-a fixing plate, 18-an air blower, 19-an air duct baffle, 20-a fan, 21-a cold water radiator, 22-a PCR detection module, 23-a fan, 24-a first air duct and 25-a cover plate.
Detailed Description
The technical solutions of the present invention will be described in detail with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without any inventive work belong to the protection scope of the present invention.
The terms "left side", "right side", "upper side" and "lower side" used in the present invention are set forth for convenience of describing structural features in conjunction with the accompanying drawings, and do not limit the scope of the present invention.
The invention provides a full-automatic nucleic acid extraction and PCR detection integrated machine, which comprises a machine shell 1, a nucleic acid extraction module and a PCR detection module 22, wherein the nucleic acid extraction module and the PCR detection module 22 are arranged in the machine shell 1, as shown in figure 2, the nucleic acid extraction module is arranged on the left side of the machine shell 1, and the PCR detection module 22 is arranged on the right side of the machine shell 1; a lifting door component for separating the nucleic acid extraction module from the PCR detection module 22; the nucleic acid extraction module comprises a consumable unit 2, a liquid transfer unit 3 and a magnetic adsorption unit 4, wherein the consumable unit 2 is arranged at the leftmost side of the nucleic acid extraction module and is used for storing consumables required by nucleic acid extraction and PCR reaction, and the consumables comprise reagents, tip heads, reaction tubes, cracking tubes, nucleic acid extraction tubes and the like; the liquid transfer unit 3 is arranged between the consumable unit 2 and the PCR detection module 22 and is used for picking up the tip head 302 and sucking the reagent and the sample in the consumable unit 2 into the tip head through the tip head 302; the magnetic adsorption unit 4 is provided below the pipetting unit 3, the magnetic adsorption unit 4 includes a magnet 401, and the magnet 401 is close to the tip head 302 for magnetic adsorption.
In the nucleic acid extraction process, if different nucleic acid extractions need to be performed simultaneously, the types of magnetic beads used for nucleic acid extraction and the adsorption time may be different, and in the prior art, when different nucleic acid extractions need to be performed simultaneously, the experiment with the longest magnetic adsorption time is often considered, so that the experiment with a shorter magnetic adsorption time requirement has a condition of too long magnetic adsorption time, which results in that the nucleic acid extraction experiment cannot be performed simultaneously, or the experiment result is not good. The invention can perform repeated magnetic adsorption, so that an experiment with short magnetic adsorption time can be considered firstly, and after the experiment with short magnetic adsorption time is completed, waste liquid or residual liquid can be transferred through the tip head, and then the waste liquid of the experiment with long magnetic adsorption time is adsorbed again, and then the experiment is performed again to complete the experiment. Furthermore, the invention can be compatible with various types of nucleic acid extraction experiments and can be carried out simultaneously, thereby greatly reducing the experiment time and the experiment cost.
As shown in fig. 3 and 4, the pipetting unit 3 includes a support frame 301, two ends of the support frame 301 are fixed on the casing 1 through screws, two sets of linear guide rails are arranged on the left side of the support frame 301, the linear guide rails are arranged along the vertical direction, two sliding blocks are arranged on each linear guide rail, the lance discharging support 303 is fixedly connected with the sliding blocks through a fixing plate, and then the lance discharging support 303 slides relative to the support frame 301 along the vertical direction. A row of through holes with equal intervals are formed in the gun arranging support 303, a connecting pipe 304 is fixed in each through hole, the connecting pipe 304 protrudes out of the lower surface of the gun arranging support 303, the tip head 302 is connected to the lower end of the connecting pipe 304, and two O-shaped rings are further arranged on the outer surface of the lower end of the connecting pipe 304 and are beneficial to connection of the connecting pipe 304 and the tip head 302.
The tip head 302 can reciprocate up and down relative to the support frame 301 along with the rifle discharging support 303; the lower end of the support frame 301 is provided with the magnetic adsorption unit 4; as shown in fig. 5, the magnetic adsorption unit 4 includes a mounting plate 402, and the mounting plate 402 is fixedly connected to the support frame 301.
Specifically, the magnetic adsorption unit 4 further includes a magnet fixing plate 403, a rack and pinion drive mechanism, and a guide assembly. The motor 408 is arranged above the mounting plate 402, an output shaft of the motor 408 penetrates through the mounting plate 402 and extends downwards, a gear 409 is fixed on the output shaft of the motor 408, the gear 409 is located below the mounting plate 402, the gear 409 is meshed with a rack 410, the rack 410 is fixedly connected with the magnet fixing plate 403, two ends of the magnet fixing plate 403 are respectively provided with a group of guide assemblies, each guide assembly comprises a guide shaft 404 and a linear bearing 405 penetrating through the guide shaft 404, the guide shaft 404 penetrates through the magnet fixing plate 403, and the linear bearing 405 is fixedly connected with the magnet fixing plate 403.
A fixing frame 406 is arranged below the mounting plate 402, two ends of the fixing frame 406 are provided with folding edges which are bent downwards, and two ends of the guide shaft 404 are fixed on the folding edges at the two ends of the fixing frame 406. The motor 408 drives the magnet fixing plate 403 to reciprocate along the guide shaft 404 through a rack-and-pinion mechanism, and approaches or departs from the tip head 302.
A spring 407 penetrates through the guide shaft 404, an end seat 413 is arranged at one end, away from the magnet 401, of the guide shaft 404, one end of the spring 407 is in contact with the linear bearing 405, and the other end of the spring 407 is in contact with the end seat 413. The purpose of the spring 407 is to prevent the magnet fixing plate 403 from deflecting due to the biasing force of the spring, which may cause the magnet to be misaligned with the tip head 302 and affect magnetic attraction.
The magnet fixing plate 403 is close to the side surface of the tip head 302 is provided with a row of grooves at equal intervals, the magnets 401 are arranged in the grooves, and the magnets correspond to the tip heads 302 one to one. The lower surface at mount 406 sets up opto-coupler 411, sets up the connecting plate on magnet fixed plate 403, sets up opto-coupler response piece 412 on the connecting plate, and opto-coupler 411 detects the displacement distance of magnet fixed plate 403 in proper order with the setting that corresponds of opto-coupler response piece 412.
As shown in fig. 1 and 6, a first air inlet 5 is arranged above the casing 1, a first air outlet 6 is arranged below the rear side of the casing 1, a second air inlet 7 is arranged above the first air outlet 6, and a second air outlet 8 is arranged above the second air inlet 7; a first air duct 24 is arranged between the lower part of the nucleic acid extraction module and the bottom wall of the machine shell 1, air is fed from a first air inlet 5 into the first air duct 24, and air is discharged from a first air outlet 6 through the nucleic acid extraction module;
a third air inlet 9 is arranged at the bottom of the machine shell 1, and outside air enters from the third air inlet 9, flows into the air path at the rear side of the first air duct 24 and then is discharged from the first air outlet 6; as different arrows in fig. 6 indicate different air flow directions.
The PCR detection module is internally provided with a second air duct, air is fed into the second air duct from a second air inlet 7, and air is discharged from a second air outlet 8 through the PCR detection module 22. The influence of outside air on the PCR detection module is effectively blocked, and meanwhile, the influence of polluted air generated in the PCR detection process on the external environment is effectively avoided.
The first air inlet 5 is provided with a first filter screen 10, the first air outlet 6 is provided with a second filter screen 11, the second air inlet 7 is provided with a third filter screen 12, and the second air outlet 8 is provided with a fourth filter screen 13.
As shown in fig. 7 and 8, the first filter 5 is fixed to the inner wall above the cabinet 1 by a frame 14. A sealing plate 15 is arranged between the upper part of the nucleic acid extraction module and the machine shell 1, the right end of the sealing plate 15 is bent upwards and connected with the machine shell 1, and the left end of the sealing plate 15 extends downwards, so that external air enters the machine shell 1 from the first air inlet 5 and then enters the consumable unit 2 downwards along the sealing plate 15; a first air duct 24 is arranged below the consumable unit 2, a fifth filter screen 16 is arranged at the left end of the first air duct 24, the fifth filter screen 16 is fixedly connected to the first air duct 24 through a fixing plate 17, and circulating air enters the first air duct 24 from the fifth filter screen 16; the right end of the first air duct 24 is provided with an air blower 18, the air duct is sealed by a cover plate 25 above the air blower 18, and the right side of the first air duct 24 is sequentially provided with an air duct baffle 19, a fan 20, a cold water radiator 21 and a second filter screen 11.
A fan 23 is arranged below the consumable unit 2, the fan 23 can promote air entering from the first air inlet 5 to rapidly enter the first air duct 24, and the air entering the first air duct 24 sequentially passes through the air duct baffle 19, the fan 20, the cold water radiator 21 and the second filter screen 11 and is discharged from the first air outlet 6. Thereby effectively blocking the outside air of instrument and influencing nucleic acid extraction, effectively avoiding simultaneously that the contaminated air that produces in the nucleic acid extraction process causes the pollution to the external environment.
The casing 1 below the air duct baffle 19 is provided with a third air inlet 9, and outside air enters from the third air inlet 9, passes through the air duct baffle 19, the fan 20, the cold water radiator 21 and the second filter screen 11 in sequence and is discharged from the first air outlet 6. The smoothness of the whole water cooling circulation system is ensured, and the risk of blockage of the PCR circulation efficiency is reduced.
As shown in FIGS. 4 and 9, a pipetting motor 305 is fixed above the middle part of the support frame 301, and the pipetting motor 305 drives the discharge gun support 303 to reciprocate up and down through a transition assembly. The transition assembly comprises an adapter plate 306, the pipetting motor 305 is connected with the adapter plate 306 through a lead screw 307 and a lead screw nut 312 and drives the adapter plate 306 to move up and down; the two ends of the adapter plate 306 are arranged on the gun arranging support 303 through a buffer component. The buffer assembly comprises a fixing screw 308 and a spring 309 arranged on the fixing screw 308 in a penetrating mode, fixing holes are formed in two ends of the adapter plate 306, and the fixing screw 308 penetrates through the fixing holes of the adapter plate 306 to be connected with the gun arranging support 303.
A needle withdrawing plate 310 is arranged below the gun arranging support 303, a plurality of through holes are formed in the needle withdrawing plate 310, and the connecting pipe 304 penetrates through the through holes; the needle withdrawing motor 311 is arranged on the supporting frame 301, and the needle withdrawing motor 311 drives the needle withdrawing plate to move up and down through a lead screw. Specifically, the two sides of the liquid-transferring motor 305 are respectively provided with a needle withdrawing motor 311, the output shaft of the needle withdrawing motor 311 is a lead screw, the lead screw of the needle withdrawing motor 311 is in threaded connection with the needle withdrawing plate 310, and then the needle withdrawing motor 311 can drive the needle withdrawing plate 310 to move up and down. the diameter of the connecting position of the tip head 302 and the connecting pipe 304 is larger than that of the through hole on the needle withdrawing plate 310, so when the needle withdrawing plate 310 moves downwards, the needle withdrawing plate 310 can push the tip head 302 to move downwards, and the tip head 302 is separated from the connecting pipe 304.
Although the present invention has been described in detail with reference to examples, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the scope of the present invention as set forth in the claims below.
Claims (14)
1. A full-automatic nucleic acid extraction and PCR detection integrated machine comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the nucleic acid extraction module and the PCR detection module are arranged in the machine shell, and a lifting door assembly for separating is arranged between the nucleic acid extraction module and the PCR detection module; the nucleic acid extraction module is characterized by comprising a consumable unit, a liquid transfer unit and a magnetic adsorption unit, wherein the consumable unit is used for storing consumables required by nucleic acid extraction and PCR reaction; the liquid transfer unit is arranged between the consumable unit and the PCR detection module and used for picking up the tip head and sucking the reagent and the sample in the consumable unit into the tip head through the tip head; the magnetic adsorption unit is arranged below the pipetting unit and comprises a magnet, and the magnet is close to the tip head for magnetic adsorption.
2. The all-in-one machine for full-automatic nucleic acid extraction and PCR detection according to claim 1, wherein the pipetting unit comprises a support frame, the support frame is fixed on the casing, a tip head is arranged on one side of the support frame, and the tip head reciprocates up and down along the support frame; the lower end of the support frame is provided with the magnetic adsorption unit; the magnetic adsorption unit comprises a mounting plate, and the mounting plate is fixedly connected with the support frame.
3. The integrated machine for full-automatic nucleic acid extraction and PCR detection according to claim 2, wherein the magnetic adsorption unit further comprises a linear driving device, a guide assembly, a magnet fixing plate and a magnet, the linear driving device is fixedly connected to the mounting plate, and the linear driving device is used for driving the magnet fixing plate to reciprocate; the guide assembly comprises a guide shaft and a linear bearing which is arranged on the guide shaft in a penetrating manner, the guide shaft is arranged on the magnet fixing plate in a penetrating manner, and the linear bearing is fixedly connected with the magnet fixing plate; the magnet is arranged on the magnet fixing plate.
4. The integrated machine for full-automatic nucleic acid extraction and PCR detection according to claim 3, wherein a fixing frame is arranged below the mounting plate, two ends of the fixing frame are provided with folded edges which are bent downwards, and the guide shaft is fixed on the folded edges at the two ends of the fixing frame; and a spring penetrates through the guide shaft, one end of the spring is contacted with the linear bearing, and the other end of the spring is contacted with the folded edge far away from the magnet.
5. The integrated machine for automatic nucleic acid extraction and PCR detection according to claim 3, wherein the linear driving device comprises a motor, a gear and a rack, the motor is disposed on the mounting plate, the output end of the motor is fixedly connected to the gear and drives the gear to rotate, the rack is engaged with the gear, and the rack is fixedly connected to the magnet fixing plate.
6. The all-in-one machine for full-automatic nucleic acid extraction and PCR detection as claimed in claim 3, wherein a plurality of grooves are formed in the side face, close to the tip head, of the magnet fixing plate at equal intervals, the magnets are arranged in the grooves, and the magnets correspond to the tip heads one to one.
7. The integrated machine for full-automatic nucleic acid extraction and PCR detection according to claim 1, wherein a first air inlet is arranged above the casing, a first air outlet is arranged below the rear side of the casing, a second air inlet is arranged above the first air outlet, and a second air outlet is arranged above the second air inlet; a first air duct is arranged between the lower part of the nucleic acid extraction module and the bottom wall of the shell, air is fed from a first air inlet through the first air duct, and air is discharged from a first air outlet through the nucleic acid extraction module;
the bottom of the shell is provided with a third air inlet, and outside air enters from the third air inlet, converges into the air path behind the first air duct and then exits from the first air outlet;
and a second air duct is arranged in the PCR detection module, air is fed from a second air inlet through the second air duct, and air is discharged from a second air outlet through the PCR detection module.
8. The integrated machine for automatic nucleic acid extraction and PCR detection according to claim 7, wherein a first filter screen is disposed at the first air inlet, a second filter screen is disposed at the first air outlet, a third filter screen is disposed at the second air inlet, and a fourth filter screen is disposed at the second air outlet.
9. The integrated machine for full-automatic nucleic acid extraction and PCR detection according to claim 8, wherein a sealing plate is arranged between the upper part of the nucleic acid extraction module and the casing, the right end of the sealing plate is bent upwards and connected with the casing, and the left end of the sealing plate extends downwards, so that external air enters the inside of the casing from the first air inlet and then enters the consumable unit downwards along the sealing plate; a first air channel is arranged below the consumable unit, a fifth filter screen is arranged at the left end of the first air channel, and circulating air enters the first air channel from the fifth filter screen;
the right end of the first air duct is provided with an air blower, and the right side of the first air duct is sequentially provided with an air duct baffle, a fan, a cold water radiator and a second filter screen.
10. The machine of claim 9, wherein a third air inlet is disposed on the housing below the air duct baffle, and outside air enters the third air inlet, passes through the air duct baffle, the fan, the cold water radiator, the second filter screen in sequence, and exits the first air outlet.
11. The full-automatic integrated nucleic acid extraction and PCR detection machine according to claim 2, wherein a gun arranging bracket is arranged on one side of the support frame, the gun arranging bracket is connected with the support frame in a sliding manner, a plurality of connecting pipes are arranged on the gun arranging bracket, and the tip head is arranged below the connecting pipes;
the liquid-transfering motor is fixed above the middle part of the support frame and drives the gun-arranging support to reciprocate up and down through the transition assembly.
12. The all-in-one machine for fully automatic nucleic acid extraction and PCR detection according to claim 11, wherein the transition assembly comprises an adapter plate, and the pipetting motor is connected with the adapter plate through a lead screw and drives the adapter plate to move up and down; the two ends of the adapter plate are arranged on the gun arranging support through the buffer assemblies.
13. The integrated machine for automatic nucleic acid extraction and PCR detection according to claim 12, wherein the buffer assembly comprises a fixing screw and a spring penetrating the fixing screw, fixing holes are provided at two ends of the adapter plate, and the fixing screw penetrates the fixing hole of the adapter plate to be connected with the gun rack.
14. The machine of claim 13, wherein a pin returning plate is disposed below the gun arranging support, the pin returning plate is provided with a plurality of through holes, and the connecting pipes are inserted into the through holes; the needle withdrawing mechanism is characterized in that a needle withdrawing motor is arranged on the supporting frame, and the needle withdrawing motor drives the needle withdrawing plate to move up and down through a lead screw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211260269.4A CN115747025B (en) | 2022-10-14 | 2022-10-14 | Full-automatic nucleic acid extraction and PCR detection all-in-one |
Applications Claiming Priority (1)
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