CN217809476U

CN217809476U - Nucleic acid extraction instrument

Info

Publication number: CN217809476U
Application number: CN202120636713.2U
Authority: CN
Inventors: 姚佳; 李金泽; 周连群; 李传宇; 张威; 周永战; 王天一; 周恒�; 周颂
Original assignee: Suzhou Sasens Co ltd
Current assignee: Suzhou Sasens Co ltd
Priority date: 2021-03-29
Filing date: 2021-03-29
Publication date: 2022-11-15
Anticipated expiration: 2031-03-29

Abstract

The utility model relates to a nucleic acid detects the field, concretely relates to nucleic acid extraction appearance, include: the temperature control device comprises a Peltier patch and a first heat conduction plate, and heat generated by the Peltier patch is transferred to the sample tube through the first heat conduction plate; a nucleic acid extraction mechanism for extracting nucleic acid in the sample tube; wherein the Peltier patch is located on one side of the first heat-conducting plate, and the nucleic acid extraction mechanism is fixed on the other side of the first heat-conducting plate. The Peltier chip has the functions of refrigeration and heating, so that the temperature can be accurately controlled, nucleic acid and a nucleic acid reagent cannot be damaged due to overhigh temperature, the nucleic acid extraction efficiency cannot be too low due to too low temperature, and the temperature of the sample tube can be timely controlled. And because no fan is required to be installed, the whole nucleic acid extractor has a simpler structure.

Description

Nucleic acid extraction instrument

Technical Field

The utility model relates to a nucleic acid detects the field, concretely relates to nucleic acid extraction appearance.

Background

Nucleic acid is a biological macromolecular compound formed by polymerizing a plurality of nucleotides, widely exists in all animal and plant cells and microorganisms, and belongs to one of the most basic substances of life.

In the detection of nucleic acids, it is generally necessary to extract the nucleic acids from the cells first. In the process of nucleic acid extraction, the influence of temperature is very large, and too high temperature can destroy nucleic acid and nucleic acid extraction reagent, and too low temperature can result in too low nucleic acid extraction efficiency.

At present, the temperature is usually adjusted by a heating plate and a fan in the process of extracting nucleic acid, but the whole structure of the nucleic acid extracting instrument is too complex due to the complex structure of the fan, and the heat dissipation effect of the fan is limited, so that ideal temperature control cannot be timely performed on the nucleic acid extracting instrument.

SUMMERY OF THE UTILITY MODEL

Therefore, the to-be-solved technical problem of the utility model lies in overcoming among the prior art nucleic acid extraction appearance overall structure too complicated, can not in time carry out the defect of ideal accuse temperature to nucleic acid extraction appearance to a nucleic acid extraction appearance is provided for extract the nucleic acid of predetermineeing the temperature from the sample cell, include:

the temperature control device comprises a Peltier patch and a first heat conduction plate, and heat generated by the Peltier patch is transferred to the sample tube through the first heat conduction plate;

a nucleic acid extraction mechanism for extracting nucleic acid in the sample tube;

wherein the Peltier patch is located on one side of the first heat-conducting plate, and the nucleic acid extraction mechanism is fixed on the other side of the first heat-conducting plate.

Preferably, a temperature sensor is arranged on the first heat-conducting plate and used for monitoring the temperature of the sample tube.

Preferably, the temperature control device further comprises a second heat-conducting plate, the first and second heat-conducting plates being located on opposite sides of the peltier chip, the second heat-conducting plate being for heat exchange with the peltier chip.

Preferably, the temperature control device further comprises at least two temperature insulation plates, and the two temperature insulation plates are arranged on two opposite sides of the temperature control device.

Preferably, the first heat-conducting plate is provided with at least one groove, and the groove is suitable for placing the sample tube.

Preferably, the device further comprises a fastening device for fixing the sample tube on the side of the first heat conduction plate far away from the Peltier patch.

Preferably, the fastening means comprises a fixing plate having a channel, the temperature control means being located within the channel, and a fastener located on at least one side wall of the channel for fixing the sample tube on the side of the first heat-conducting plate remote from the peltier chip.

Preferably, the nucleic acid extraction mechanism comprises a magnetic rod assembly and a magnetic sleeve assembly, and the magnetic rod assembly and the magnetic sleeve assembly are matched for extracting the nucleic acid in the sample tube;

the magnetic rod assembly comprises a magnetic rod mounting frame and a magnetic rod arranged on the magnetic rod mounting frame;

the magnetic sleeve assembly comprises a magnetic sleeve mounting rack, and the magnetic sleeve mounting rack is used for placing the magnetic sleeve.

Preferably, the sensor device further comprises a support plate, a slide rail, a first sensor and a second sensor are arranged on the support plate, the first sensor and the second sensor are distributed on two sides of the slide rail in a staggered mode, and the magnetic bar mounting rack and the magnetic sleeve mounting rack are arranged on the slide rail in a sliding mode;

the magnetic bar assembly further comprises a second induction part arranged on the side of the magnetic bar mounting frame, and the magnetic sleeve assembly further comprises a first induction part arranged on the side of the magnetic sleeve mounting frame.

The utility model discloses technical scheme has following advantage:

1. the utility model provides a nucleic acid extraction appearance, first heat-conducting plate transmit the heat (air conditioning or steam) that the peltier piece produced for the sample cell to heat up or cool down the sample cell, and then the temperature of maintaining the sample cell is in and predetermines the temperature. The Peltier patch has the functions of refrigerating and heating at the same time, so that the temperature can be accurately controlled, nucleic acid and a nucleic acid reagent cannot be damaged due to overhigh temperature, the nucleic acid extraction efficiency cannot be too low due to too low temperature, and the temperature of the sample tube can be timely controlled. And because no fan is required to be installed, the whole nucleic acid extractor has a simpler structure.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic view of the nucleic acid extractor of the present invention;

FIG. 2 is a schematic view of the temperature control apparatus of FIG. 1;

FIG. 3 is another schematic view of the temperature control device of FIG. 1;

FIG. 4 is a schematic view of another configuration of the temperature control device of FIG. 1;

FIG. 5 is a schematic view showing the structure of a nucleic acid isolation mechanism and a support plate;

FIG. 6 is a schematic view of the nucleic acid extractor of the present invention with the outer shell removed;

FIG. 7 is a schematic view of the structure of the magnetic rod assembly and the magnetic sleeve assembly;

FIG. 8 is a schematic view of the structure of the magnetic rod assembly separated from the magnetic sleeve assembly;

FIG. 9 is a hardware block diagram of the nucleic acid extractor of the present invention;

FIG. 10 is a software flowchart of the nucleic acid extractor of the present invention;

FIG. 11 is a flow chart of the control logic of the nucleic acid extractor of the present invention.

101. A second heat-conducting plate; 102. a fastening assembly; 1021. a fastener mount; 1022. a fastener; 103. a Peltier patch; 104. a first heat-conducting plate; 1041. a groove; 105. a fixing plate; 106. a heat insulation plate; 107. a temperature sensor; 201. a support plate; 2011. a slide rail; 2012. a first sensor; 2013. a first sensing part; 2014. a second sensing part; 2015. a second sensor; 202. a connecting member; 203. a magnetic bar mounting rack; 2031. a magnetic bar; 204. a magnetic sleeve mounting rack; 301. a first base plate; 302. A foot pad; 303. a rear bracket; 3031. a limiting seat; 304. a motor bracket; 305. a second base plate; 401. a housing.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the two elements may be directly connected or indirectly connected through an intermediate medium, or may be communicated with each other inside the two elements, or may be wirelessly connected or wired connected. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.

Furthermore, the technical features mentioned in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

At present, the extraction of nucleic acid is usually realized by transferring magnetic beads, in the process, an operating system can control the flow and temperature of the extraction, and the general process of the extraction is as follows:

1) Cracking: adding lysis solution into the sample, mixing and reacting the reaction solution by mechanical movement and heating, lysing cells, and releasing nucleic acid.

2) Adsorption: adding magnetic beads into the sample lysis solution, fully and uniformly mixing, adsorbing nucleic acid by utilizing the characteristic that the magnetic beads have strong affinity to the nucleic acid under high salt and low pH values, separating the magnetic beads from the solution under the action of an external magnetic field, and enabling the magnetic beads and the nucleic acid to enter the next operation station.

3) Washing: and (3) fully and uniformly mixing the magnetic beads, the nucleic acid and the washing solution in a new sample tube, washing the nucleic acid and removing impurities.

4) And (3) elution: removing the external magnetic field, eluting in buffer solution, mixing uniformly, and separating nucleic acid from magnetic beads to obtain purified nucleic acid.

Temperature is a critical external factor in the extraction of nucleic acids. Too high a temperature will destroy nucleic acids and nucleic acid reagents, while too low a temperature will affect the efficiency of nucleic acid extraction.

Example 1

This embodiment provides a nucleic acid extraction apparatus, which can be used to extract nucleic acid at a predetermined temperature from a sample tube (also can be a deep well plate), wherein the predetermined temperature can be a temperature range, and can also be a specific temperature value, and in this embodiment, the predetermined temperature is preferably a temperature range.

As shown in FIGS. 1 to 4, the nucleic acid extracting apparatus comprises a temperature control device including a Peltier chip 103 and a first heat conducting plate 104, the Peltier chip 103 being located on one side of the first heat conducting plate 104, and a nucleic acid extracting mechanism fixed on the other side of the first heat conducting plate 104. Because the peltier sheet 103 has bidirectionality, the same side can be used for heating and cooling, namely, when the peltier sheet 103 is used for heating at the side close to the first heat conduction plate 104, the side of the peltier sheet 103 far away from the first heat conduction plate 104 is used for cooling; when the peltier element 103 is cooled on the side close to the first heat conduction plate 104, the peltier element 103 is heated on the side far from the first heat conduction plate 104.

In actual use, a sample tube (not shown) containing a nucleic acid sample to be extracted is fixed on one side of the first heat conducting plate 104 far away from the peltier element 103, and when the peltier element 103 is cooled or heated on one side close to the first heat conducting plate 104, the first heat conducting plate 104 transfers heat (including hot gas and hot gas) generated by the peltier element 103 to the sample tube, so that the temperature of the sample tube is reduced or increased. The nucleic acid extraction mechanism is used for extracting nucleic acid from a sample in the sample tube, and the Peltier 103 is used for accurately controlling the temperature of the sample tube, so that the temperature in the sample tube can be maintained at a preset temperature when the nucleic acid extraction mechanism is used for extracting nucleic acid from the sample.

In the above embodiment, the first heat conducting plate 104 transfers the heat (cold air or hot air) generated by the peltier element 103 to the sample tube to heat or cool the sample tube, so as to maintain the temperature of the sample tube at a predetermined temperature. The Peltier patch 103 has the functions of refrigerating and heating at the same time, so that accurate temperature control can be achieved, nucleic acid and nucleic acid reagents cannot be damaged due to overhigh temperature, nucleic acid extraction efficiency cannot be too low due to too low temperature, and temperature control can be timely performed on a sample tube. And because no fan is required to be installed, the whole nucleic acid extractor has a simpler structure.

In order to monitor the temperature in the sample tube in time, as shown in fig. 4, a temperature sensor 107 is disposed on the first heat conducting plate 104, and the temperature sensor 107 can be used to detect the temperature of the sample tube, so as to avoid the over-low/over-high temperature condition of the sample tube. In some embodiments, a temperature sensor, such as an infrared temperature sensor, can also be provided on the nucleic acid extraction mechanism.

When the peltier element 103 is operated, the faster the cooling/heat dissipation speed of the side far away from the first heat conduction plate 104 is, the faster the heating/cooling speed of the side near the first heat conduction plate 104 is, i.e. the higher the cooling/heat dissipation efficiency of the side far away from the first heat conduction plate 104 of the peltier element 103 is, the higher the heating/cooling efficiency of the side near the first heat conduction plate 104 is. Thus, as shown in fig. 2-4, the temperature control device further comprises a second heat conducting plate 101, the first 104 and second 101 heat conducting plates being located on opposite sides of the peltier patch 103. The second heat conduction plate 101 may be a large-area metal plate, such as an aluminum plate, an iron plate, or a copper plate.

When cold air or hot air is generated on one side of the peltier element 103 close to the second heat conducting plate 101, the second heat conducting plate 101 exchanges heat with the peltier element 103, and the cold air or the hot air generated by the peltier element 103 is rapidly transmitted out, so that the cold/heat dissipation efficiency of the peltier element 103 close to one side of the second heat conducting plate 11 is improved, the heating/cooling effect of the peltier element 103 close to one side of the first heat conducting plate 104 is further improved, and finally, the temperature of the sample tube is controlled efficiently and ideally.

In order to prevent the loss of hot/cold air generated by the peltier element 103, a thermal insulation material may be disposed around the peltier element 103. In the present embodiment, as shown in fig. 2-5, at least two thermal insulation plates 106 are included, and the thermal insulation plates 106 may be made of bakelite, polypropylene, or the like. Two thermal barriers 106 are provided on opposite sides of the temperature control device to surround the temperature control device. In some embodiments, the thermal barriers 106 may be eight, ten, etc., and an equal number of thermal barriers 106 may be disposed on opposite sides of the temperature control device.

As shown in fig. 2-4, the first heat-conducting plate 104 has a row of grooves 1041, and the number of the grooves 1041 may be one, two, five, seven, or the like. The groove 1041 can be used for placing a sample tube, the groove 1041 can increase the contact area between the first heat conduction plate 104 and the sample tube, and when the first heat conduction plate 104 transfers the heat generated by the peltier element 103 close to one side of the first heat conduction plate 104 to the sample tube, the groove 1041 can increase the transfer efficiency of the first heat conduction plate 104, so as to rapidly refrigerate or heat the sample tube, and make the sample and the nucleic acid reagent better heated or refrigerated.

The fastening device can be used for fixing the sample tube on one side of the first heat conduction plate 104 far away from the Peltier plate 103, and can avoid the shaking of the nucleic acid extraction instrument in the working process due to the operation of a motor and the like.

In the embodiment, as shown in fig. 2, the fastening device includes a fixing plate 105 having a channel and a fastening assembly 102 disposed on at least one side wall of the channel, the fastening assembly 102 includes a fastener mount 1021 and a fastener 1022 fixed on the fastener mount 1021, the fastener mount 1021 is fixed on the side wall of the channel, and the fastener 1022 may have an arch shape, a trapezoid shape, or the like. The temperature control device is positioned in the channel, and the sample tube positioned on the groove 1041 is fixed under the clamping of the fasteners 1022 on the two opposite sides. In some embodiments, the fastening means may also be a double-sided tape, a clip, or the like.

As shown in FIGS. 5-8, the nucleic acid extraction mechanism comprises a magnetic rod assembly and a magnetic sleeve assembly, which are cooperated with each other to extract nucleic acid in the sample tube. Be provided with slide rail 2011 in the backup pad 201, slide rail 2011 both sides dislocation distribution has first sensor 2012 and second sensor 2015, and second sensor 2015 is located first sensor 2012 top. The magnetic rod assembly comprises a magnetic rod mounting frame 203 and a magnetic rod 2031 mounted on the magnetic rod mounting frame 203, the magnetic sleeve assembly comprises a magnetic sleeve mounting frame 204, and a magnetic sleeve (not shown) can be placed on the magnetic sleeve mounting frame 204. The bar magnet subassembly is located the magnetism cover subassembly top, and bar magnet 2031 wears to establish in the magnetism cover during the use, avoids the sample to pollute.

The magnetic rod mounting rack 203 is provided with a magnetic rod array, the magnetic sleeve mounting rack 204 can be used for placing the magnetic sleeve array, the magnetic rod array is matched with the magnetic sleeve array to extract nucleic acid in the sample tubes, and the nucleic acid extraction can be simultaneously carried out on a plurality of sample tubes. For example, in this embodiment, 16 magnetic rods are fixed on the magnetic rod mounting bracket 203, 16 magnetic sleeves can be placed on the magnetic sleeve mounting bracket 204, and the magnetic rods and the magnetic sleeves are matched in a one-to-one correspondence manner.

The magnetic rod mounting bracket 203 and the magnetic sleeve mounting bracket 204 are slidably disposed on the slide rail 2011, and a second sensing portion 2014 is disposed on the magnetic rod mounting bracket 203 side, and a first sensing portion 2013 is disposed on the magnetic sleeve mounting bracket 204 side. In this embodiment, the first sensor 2012 and the second sensor 2015 may be photosensors, the photosensors are fixed on the supporting plate 201 by a photosensor holder, and the first sensing portion 2013 and the second sensing portion 2014 may be metal sheets. In some embodiments, the first sensor 2012 and the second sensor 2015 may also be distance sensors.

When the bar magnet mounting bracket 203 slides to the second sensor 2015 through the slide rail 2011, the second sensor 2015 senses the second sensing part 201; when the magnetic sleeve mounting rack 204 slides to the first sensor 2012 through the slide rail 2011, the first sensor 2012 senses the first sensing part 2013.

As shown in FIGS. 1 and 6, a second base plate 305 having a through-hole is further included, the fastening means is located in the through-hole of the second base plate 305, the support plate 201 is fixed to the second base plate 305, a rear holder 303 is further fixed to the second base plate 305, and the rear holder 303 and the nucleic acid extracting mechanism are located on opposite sides of the support plate 201. The rear support 303 is provided with a motor support 304 and a limiting seat 3031, the motor support 304 is arranged on the edge of the upper plate of the rear support 303, the limiting seat 3031 is fixed on the upper plate, and the upper surface of the limiting seat 3031 is recessed inwards to form a section of limiting groove.

The motor bracket 304 may be fixed with a motor, wherein the motor may be a stepper motor, a servo motor, a linear motor, or the like. One side of the supporting plate 201 close to the rear bracket 303 is provided with a connecting piece, and the connecting piece can be connected with the driving end of the motor so as to move back and forth in the limiting groove under the driving of the motor.

As shown in FIGS. 1 and 6, the nucleic acid extracting mechanism may be covered with a casing 401 to prevent contamination of the nucleic acid during the extraction process and abnormal movement of the nucleic acid extracting mechanism. The second base plate 305 is fixed on the first base plate 301, the first base plate 301 and the second base plate 305 are provided with a pad 302 opposite to each other, and the pad 302 is used as a buffer device to prevent the nucleic acid extraction instrument from slipping and shaking in operation.

As shown in FIG. 9, a power supply 501 supplies power to the nucleic acid extractor, and a main control board 502 serves as a control board of the nucleic acid extractor and is responsible for controlling a motion control module 504, a display 503 and a temperature control module 506. The display 503 is used for data display/user interaction, that is, the display 503 can be used to display the motion steps, the current operation time, the remaining time, the temperature of the heating region, etc. of the nucleic acid extractor, and also can be used as an input terminal for user interaction. The display 503 may be selected from a liquid crystal touch screen, a nixie tube, a 12864 screen, keys, and the like.

The temperature control module 506 can control the peltier element 103 and the temperature sensor 107, the peltier element 103 serves as a heating element to heat/cool the sample tube, the temperature sensor 107 detects the temperature of the sample tube, and when the temperature sensor 107 detects that the temperature of the sample tube is lower than a preset temperature, the temperature control module 506 controls the peltier element 103 (the side close to the first heat conducting plate 104) to heat; when the temperature sensor 107 detects that the temperature of the sample tube is higher than the preset temperature, the temperature control module 506 controls the peltier chip 103 (the side close to the first heat conduction plate 104) to refrigerate.

The motion control module 504 controls the motor 505 and the photoelectric sensor 507, and the motion control module 504 may be a single chip microcomputer, an FPGA, or the like. A magnetic rod motion motor 5051 is used for controlling the motion of a magnetic rod so as to realize key operations such as cracking, adsorption, washing, elution and the like in nucleic acid extraction, a magnetic sleeve motion motor 5052 is used for controlling the motion of a magnetic sleeve, and the magnetic sleeve is used for isolating the magnetic rod and a sample so as to prevent the sample from being polluted; the moving motor 5053 is used for driving the magnetic bar moving motor 5051 and the magnetic sleeve moving motor 5052 to move between different stations.

When the photoelectric sensors (for example, the first sensor 2012 and the second sensor 2015 in fig. 8) detect that the magnetic rod and the magnetic sleeve move to a certain position, the motion control module 504 controls the magnetic rod motion motor 5051 and the magnetic sleeve motion motor 5052 to stop moving, so that the magnetic rod and the magnetic sleeve are limited to prevent abnormal work.

As shown in FIG. 10, when nucleic acid is extracted by the nucleic acid extractor, the nucleic acid extractor can be controlled by software to operate according to the following steps:

step S701 is performed to turn on the power supply to energize the nucleic acid extractor.

Executing step S702, if the software can not normally enter, executing step S703, and displaying a screen on a display to report an error ERR so as to remind an operator of timely repairing; if the software can enter normally, the nucleic acid extractor can operate normally, and then step S704 is performed.

After the nucleic acid extractor is operating normally, step S704 provides the user with three seed program choices: step S705 is performed, step S706 is performed, and step S707 is performed. Step S705, step S706 and step S707 correspond to the extraction time and temperature of different reagents, respectively, i.e. step S7051 may be executed to define the flow/time after step S705 is executed, step S7061 may be executed to set the fixed flow/time after step S706 is executed, and step S7071 may be executed to set the fixed flow/time after step S707 is executed. When the set time is reached, step S708 is executed to end the process, step S709 is executed to remind the user of the end of nucleic acid extraction by sound, the end reminder is also displayed on the display and initialization is performed, and finally step S710 is executed to prepare the system for initialization.

As shown in FIG. 11, the workflow of the nucleic acid extractor is as follows:

and step S801 is executed, the power supply of the nucleic acid extractor is switched on, and the software is normally started.

Step S802 is executed, the system waits to receive the control command sent by the user, that is, the user sends an operation command, a temperature signal, and a flow signal to the system.

Step S804 is executed, which specifically includes: step S8041 is executed to detect the temperature of the sample tube; and step S8042 is executed, the detected sample tube temperature is compared with the temperature set by the user, and step S8043 is executed according to the comparison result, so that the power output is controlled. When the temperature collected by the sample tube is lower than the temperature set by a user, the Peltier is controlled to heat the extraction area, and when the detected temperature reaches the temperature set by the user, the heating of the extraction area is stopped, so that closed-loop control is realized; and when the temperature collected by the sample tube is higher than the temperature set by a user, controlling the Peltier to refrigerate the extraction area. And, the collected temperature can be transmitted to the display, and the display executes step S806 to display the temperature value on the display.

Step S803 is executed, where the motion part is a motor control module of the nucleic acid extractor, and specifically includes: after step S801 is executed, step S8031 is executed to perform automatic initialization for starting up, so that each moving component (the magnetic sleeve component, the magnetic rod component, and the like) enters an initial position. After receiving a user instruction sent by a user, step S805 is executed, the position of each moving component can be determined by a signal detected by the photoelectric sensor, and when the position of each moving component reaches a specified position, step S8051 is executed to execute movement, i.e., stop or move, on each moving component. And the current motion state, motion step and other information can be sent to the display, and the display executes step S806 to display the motion state, motion step and other information on the display.

After the operation of the nucleic acid extractor, when the temperature detected by the temperature sensor 107 is lower than the temperature set by the user, the peltier chip 103 operates to heat the first heat conduction plate 104 and refrigerate the second heat conduction plate 101. The faster the speed of cooling the second heat transfer plate 101, the higher the heating efficiency of the first heat transfer plate 104, and therefore the second heat transfer plate 101 uses a large-area metal plate as a heat radiation material. When the temperature collected by the temperature sensor 107 is higher than the temperature set by the user, the peltier element 103 works in the reverse direction to refrigerate the first heat-conducting plate 104, and the second heat-conducting plate 101 uses a large-area metal plate as a heat-dissipating material to rapidly cool the sample tube, so that the temperature of the sample tube is not excessively overshot. Thus, the reciprocating closed-loop action is realized, and the accurate control of the temperature is realized.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made without departing from the scope of the invention.

Claims

1. A nucleic acid extractor for extracting nucleic acid at a predetermined temperature from a sample tube, comprising:

the temperature control device comprises a Peltier patch (103) and a first heat conduction plate (104), and heat generated by the Peltier patch (103) is transferred to the sample tube through the first heat conduction plate (104);

wherein the Peltier chip (103) is located on one side of the first heat-conducting plate (104), and the nucleic acid extraction mechanism is immobilized on the other side of the first heat-conducting plate (104).

2. The nucleic acid extractor of claim 1, wherein the first thermal conductive plate (104) is provided with a temperature sensor (107) for monitoring the temperature of the sample tube.

3. The nucleic acid extraction apparatus according to claim 1, wherein the temperature control means further comprises a second heat-conducting plate (101), the first heat-conducting plate (104) and the second heat-conducting plate (101) being located on opposite sides of the peltier chip (103), the second heat-conducting plate (101) being for exchanging heat with the peltier chip (103).

4. The nucleic acid extractor of claim 3, further comprising at least two thermal barriers (106), wherein the two thermal barriers (106) are disposed on opposite sides of the temperature control device.

5. The nucleic acid extraction instrument of claim 1, wherein the first heat conducting plate (104) is provided with at least one recess (1041), and the recess (1041) is adapted to receive the sample tube.

6. The nucleic acid extraction apparatus of claim 1, further comprising a fastening device for securing the sample tube to a side of the first heat-conducting plate (104) remote from the peltier chip (103).

7. The nucleic acid extraction apparatus of claim 6, wherein the fastening means comprises a fixing plate (105) having a channel and a fastener (1022) on at least one side wall of the channel, the temperature control means being located within the channel, the fastener (1022) being for fixing the sample tube on a side of the first heat-conducting plate (104) remote from the Peltier plate (103).

8. The nucleic acid extractor of any one of claims 1-7, wherein the nucleic acid extraction mechanism comprises a magnetic rod assembly and a magnetic sleeve assembly, wherein the magnetic rod assembly and the magnetic sleeve assembly cooperate to extract nucleic acid in the sample tube;

the magnetic rod assembly comprises a magnetic rod mounting rack (203) and a magnetic rod (2031) mounted on the magnetic rod mounting rack (203);

the magnetic sleeve assembly comprises a magnetic sleeve mounting rack (204), and the magnetic sleeve mounting rack (204) is used for placing a magnetic sleeve.

9. The nucleic acid extractor according to claim 8, further comprising a support plate (201), wherein the support plate (201) is provided with a slide rail (2011), a first sensor (2012) and a second sensor (2015) which are distributed at two sides of the slide rail (2011) in a staggered manner, and the magnetic rod mounting rack and the magnetic sleeve mounting rack are slidably arranged on the slide rail (2011);

the bar magnet subassembly is still including setting up second response portion (2014) in bar magnet mounting bracket (203) side, the bar magnet subassembly is still including setting up first response portion (2013) in bar magnet mounting bracket (204) side.