CN114517144A - Nucleic acid extraction instrument and nucleic acid extraction method - Google Patents

Abstract

The invention provides a nucleic acid extraction instrument and a nucleic acid extraction method, which can improve the automation degree and are beneficial to reducing the cost. The nucleic acid extractor comprises a bracket, a containing table, a first position sensor assembly, a camera, a label recognition device and a controller; the accommodating table is used for accommodating the deep hole plate; a sample label is arranged on the side surface of the deep hole plate and used for indicating information of a sample associated with the deep hole plate; the first position sensor assembly is used for sensing whether the deep hole plate is accommodated in place or not; when the deep hole plate is accommodated in place, the sample label is aligned to a light inlet of the camera, and the camera is used for collecting images containing the sample label; the label identification device is used for receiving the image and identifying a sample label in the image; the controller is used for receiving the identification result, determining the category of the sample based on the identification result, and acquiring a target nucleic acid extraction program corresponding to the category of the sample from the stored multiple nucleic acid extraction programs, wherein the target nucleic acid extraction program is used for realizing the nucleic acid extraction of the sample.

Description

Nucleic acid extraction instrument and nucleic acid extraction method

technical field

The invention relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction instrument and a nucleic acid extraction method.

Background technique

Nucleic acid extraction technology is an important method to achieve precision medicine in the fields of biology and medicine. In order to avoid sample contamination and save labor costs, traditional manual experimental extraction has been gradually replaced by automated nucleic acid extraction, that is, automated nucleic acid extraction can be achieved through a preset nucleic acid extraction procedure.

Although nucleic acid extraction can be achieved through nucleic acid extraction procedures, a series of other tasks, including the selection of procedures, are required before this. In the current relevant nucleic acid extraction experiments, the experimenter pre-records the sample information and marks the deep-well plate, then puts the deep-well plate into the nucleic acid extraction instrument, manually selects the corresponding nucleic acid extraction procedure, completes the nucleic acid extraction, and finally according to the Corresponding sample information records and deep-well plate markings are used for subsequent experiments.

In the above method, the entire experimental process is not fully automated, which is relatively labor-intensive and time-consuming, especially if there are many nucleic acid extraction procedures, such as when the nucleic acid extraction instrument has 500 procedures stored in advance, the laboratory operator manually selects nucleic acid. Extraction will be a particularly tedious process.

SUMMARY OF THE INVENTION

The present invention provides a nucleic acid extraction instrument and a nucleic acid extraction method, which can improve the degree of automation and help reduce costs.

A first aspect of the present invention provides a nucleic acid extractor, including: a bracket, a accommodating table, a first position sensor assembly, a camera, a label identification device, and a controller;

The accommodating table is arranged on the bracket and is used for accommodating the deep-well plate; the side of the deep-well plate is provided with a sample label, and the sample label is used to indicate the information of the sample associated with the deep-well plate;

The first position sensor assembly is arranged on the accommodating table, and is used for sensing whether the deep-well plate is accommodated in place;

The camera is mounted on the bracket, the sample label is aligned with the light inlet of the camera when the deep-well plate is accommodated in place, and the camera is used to collect an image containing the sample label;

The label identification device is connected to the camera, and is used for receiving the image and identifying the sample label in the image;

The controller is connected to the label identification device, and is used for receiving the identification result and determining the category of the sample based on the identification result, and obtaining the target nucleic acid corresponding to the category of the sample from a plurality of stored nucleic acid extraction programs An extraction procedure, the target nucleic acid extraction procedure is used for realizing nucleic acid extraction of the sample.

According to an embodiment of the present invention, further comprising a movable mechanism;

the movable mechanism is mounted on the bracket;

The accommodating table is installed on the movable mechanism, and can be moved back and forth relative to the camera through the movable mechanism; during the forward and backward movement of the accommodating table, the sample label is always aligned with the position of the camera. light inlet.

According to an embodiment of the present invention,

The controller is further configured to: control the camera to capture an image when it is determined that the first position sensor assembly senses that the deep-well plate is accommodated in place, if not received within a set period of time thereafter. Recognition result, then control the movable mechanism to drive the accommodating table to move relative to the camera to adjust the distance between the sample label and the camera, and when the accommodating table is moved, control the camera to collect new image;

The label identification device is further configured to: identify the sample label in the image every time the image is received, and send the identification result to the controller when the identification result is obtained.

According to an embodiment of the present invention, when the controller controls the movable mechanism to drive the accommodating platform to move relative to the camera, the controller is further configured to:

Check whether the current position of the accommodating table is the set position, or whether the direction from the set position to the current position is the first direction, and the first direction is the direction away from the camera or the direction close to the camera;

If so, when the distance between the current position and the set position is greater than or equal to the set distance, the accommodating table is controlled to be reset to the set position and then moved to the second direction by a specified distance, and the second direction is the set position. In the opposite direction of the first direction, the specified distance is less than the set distance; when the distance between the current position and the set position is less than the set distance, the accommodating table is controlled to move the specified distance in the first direction;

If not, when the distance between the current position and the set position is greater than or equal to the set distance, the accommodating table is controlled to be reset to the set position and then moved to the first direction by the specified distance; When the distance of the fixed position is less than the set distance, the accommodating table is controlled to move the specified distance in the second direction.

According to an embodiment of the present invention, further comprising a magnetic rod sleeve and a second position sensor assembly;

The magnetic rod sleeve holder is used for accommodating the magnetic rod sleeve;

The second position sensor assembly is arranged on the magnet bar sleeve frame, and is used for sensing whether the magnet bar sleeve is accommodated in place;

The controller is further configured to: run the target nucleic acid extraction program when it is determined that the second position sensor assembly senses that the magnetic rod cover is accommodated in place; otherwise, prohibit running the target nucleic acid extraction program.

According to an embodiment of the present invention,

The magnetic rod sleeve frame includes N magnetic rod sleeve accommodating parts, each magnetic rod sleeve accommodating part is used for accommodating a row of magnetic rod sleeves, and the N is greater than or equal to 1;

The second position sensor assembly includes N second position sensors, which are respectively arranged at designated positions of the N magnetic rod sleeve accommodating parts;

When the second position sensor assembly senses that the magnetic rod sleeve is accommodated in place, further: the N second position sensors all sense that the magnetic rod sleeve reaches the designated position of the magnetic rod sleeve accommodating part.

According to an embodiment of the present invention,

The accommodating table includes at least one accommodating part, and each accommodating part is used for accommodating a deep-well plate;

The first position sensor assembly includes at least two first position sensors, wherein the two first position sensors are respectively disposed at diagonal positions of the accommodating portion.

According to an embodiment of the present invention, it further includes a heating device, the heating device is fixed on the bottom of the accommodating table, and is used for heating the deep-well plate;

The heating device includes a heating strip assembly, a heat shield, a heat insulation layer and a cooling fan;

The heating strip assembly includes a heating strip body and a heating head, and the heating head is located at the upper end of the heating strip body;

The heat shield has an upper end opening and a lower end opening, the heat shield is sleeved on the heating strip assembly and protrudes from the upper end opening by the heating head;

The heat insulating layer seals the lower end opening of the heat shield, so as to form a fully enclosed space between the heat insulating layer, the heat shield and the heating head, and the heating bar body is surrounded by the heat shield. in the surrounding space;

Heat dissipation holes are provided on the heat shield and/or the heat insulation layer, and the heat dissipation fan is arranged toward at least one heat dissipation hole.

According to an embodiment of the present invention,

At least one row of heat dissipation holes is opened on at least one side of the heat shield;

At least one row of heat dissipation holes is opened on the heat insulating pressure strip.

According to an embodiment of the present invention,

The cooling fan is arranged at the bottom of the heat insulating layer and faces the heat dissipation hole of the heat insulating layer.

According to an embodiment of the present invention,

There is a gap between at least one side surface of the heating strip body and the corresponding side wall of the heat shield;

There is a gap between the bottom of the heating bar body and the heat insulating bead.

According to an embodiment of the present invention,

The bottom of the heating strip body is also provided with cooling ribs.

According to an embodiment of the present invention,

The size of the upper end opening of the heat shield matches the size of the heating head, so that the heating head can seal the upper end opening of the heat shield.

According to an embodiment of the present invention,

The cooling fan is used for not supplying air when the heating device is heating, and starting to supply air when the heating device finishes heating.

A second aspect of the present invention provides a nucleic acid extraction method, which is applied to the controller in the nucleic acid extraction apparatus described in the foregoing embodiments, and the method includes:

When it is determined that the first position sensor assembly senses that the deep-well plate is accommodated in place, the camera is controlled to capture an image; wherein, the label recognition device is capable of receiving an image captured by the camera every time , identify the sample label in the image, and send the identification result to the controller when the identification result is obtained;

Check whether the recognition result is received within a set period of time afterward;

If not, control the accommodating table to move relative to the camera to adjust the distance between the sample label and the camera, and when the accommodating table is moved, control the camera to collect a new image, and return to check the The step of whether the recognition result is received within a set period of time after that;

If so, determine the category of the sample based on the identification result, and obtain a target nucleic acid extraction program corresponding to the category of the sample from a plurality of stored nucleic acid extraction programs, and the target nucleic acid extraction program is used to realize the sample. nucleic acid extraction.

According to an embodiment of the present invention, the controlling the accommodating platform to move relative to the camera includes:

Check whether the current position of the accommodating table is the set position, or whether the direction from the set position to the current position is the first direction, and the first direction is the direction away from the camera or the direction close to the camera;

If so, when the distance between the current position and the set position is greater than or equal to the set distance, the accommodating table is controlled to be reset to the set position and then moved to the second direction by a specified distance, and the second direction is the set position. In the opposite direction of the first direction, the specified distance is less than the set distance; when the distance between the current position and the set position is less than the set distance, the accommodating table is controlled to move the specified distance in the first direction;

If not, when the distance between the current position and the set position is greater than or equal to the set distance, the accommodating table is controlled to be reset to the set position and then moved to the first direction by the specified distance; When the distance of the fixed position is less than the set distance, the accommodating table is controlled to move the specified distance in the second direction.

According to an embodiment of the present invention, after obtaining a target nucleic acid extraction program corresponding to the category of the sample from a plurality of stored nucleic acid extraction programs, the method further includes:

When it is determined that the second position sensor assembly senses that the magnetic rod cover is accommodated in place, the target nucleic acid extraction program is executed, otherwise the target nucleic acid extraction program is prohibited from running, and the second position sensor assembly is disposed on the magnetic rod. On the sleeve frame, the magnetic rod sleeve frame is used for accommodating the magnetic rod sleeve.

The present invention has the following beneficial effects:

In the embodiment of the present invention, a sample label is provided on the side of the deep well plate, and by arranging the first position sensor assembly on the accommodating table, it can sense whether the deep well plate is accommodated in place, so that the sample label of the deep well plate can be Aim at the light inlet of the camera, so that the controller can accurately control the timing of the camera to capture the image, the camera can capture the image containing the complete sample label, to avoid the problem of subsequent unrecognized caused by only collecting the image of the partial sample label, and the camera The size is smaller than that of the label recognition device, and it is easier to be set to the position of the sample label aligned with the deep well plate. Therefore, the label recognition device can be set to The spare position of the nucleic acid extraction instrument can be more conducive to the miniaturization of the entire nucleic acid extraction instrument. The label recognition device can identify the identification result of the sample label from the image and then send it to the controller, and the controller can determine according to the identification result. The required target nucleic acid extraction procedure, the target nucleic acid extraction procedure can realize the nucleic acid extraction of the sample, the whole process mentioned above is fully automated, it does not need to manually record the sample information, select the nucleic acid extraction procedure, etc., which can reduce labor and time costs, and can also Avoid manual errors and improve the accuracy of nucleic acid extraction.

Description of drawings

In order to explain the embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention, and for those of ordinary skill in the art, other drawings can also be obtained from these drawings without any creative effort.

1 and 2 are schematic structural diagrams of a nucleic acid extractor at different angles according to an embodiment of the present invention;

3 is a schematic diagram of an exploded structure of a heating device without a cooling fan according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of an assembled structure of a heating device without a cooling fan according to an embodiment of the present invention;

5 is a schematic structural diagram of a heating device provided with a cooling fan installed on a accommodating table of a nucleic acid extraction apparatus according to an embodiment of the present invention;

6 is a schematic flowchart of a nucleic acid extraction method according to an embodiment of the present invention.

Description of reference numbers:

Bracket 10; accommodating table 11; limit block 111; elastic piece 112; first position sensor 113; deep well plate 20; sample label 21; camera 30; label identification device 31; cable 32; Position sensor assembly 41; magnetic rod cover 42; heating device 50; heat insulation cover 51; upper end opening 511; first heat dissipation hole 512; heat insulation pressure strip 52; second heat dissipation hole 521; heating strip body 531; Pit 5321; heating element 533; cooling ribs 534; cooling fan 54.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

The terms "first", "second", "third", "fourth", etc. (if present) in the description and claims of the present invention and the above-mentioned drawings are used to distinguish similar objects and are not necessarily used to Describe a particular order or sequence. It is to be understood that the data so used may be interchanged under appropriate circumstances such that the embodiments of the invention described herein can be practiced in sequences other than those illustrated or described herein. Furthermore, the terms "comprising" and "having" and any variations thereof, are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to those expressly listed Rather, those steps or units may include other steps or units not expressly listed or inherent to these processes, methods, products or devices.

The technical solutions of the present invention will be described in detail below with specific examples. The following specific embodiments may be combined with each other, and the same or similar concepts or processes may not be repeated in some embodiments.

1 and 2 show a nucleic acid extractor according to an embodiment of the present invention. The nucleic acid extractor may include a bracket 10, a receiving table 11, a first position sensor assembly, a camera 30, a label identification device 31, and a controller. Of course, in addition to this, the nucleic acid extractor may also have other components, for example, may also include an actuator for performing sample nucleic acid extraction, and a drive mechanism for the actuator, etc., which are not specifically limited.

The accommodating table 11 , the camera 30 and the label identification device 31 can all be arranged on the bracket 10 . Of course, the controller can also be arranged on the bracket 10, or can also be arranged outside the bracket 10, as long as the camera 30, the label identification device 31 and some other required components (such as a driving mechanism) can be connected. The setting here may refer to installation and fixation, for example, it may be connected and fixed through a connector, and the connector here may refer to a screw connection. It is understood that the installation and fixation method here is only an example and not a limitation.

The accommodating table 11 is used for accommodating the deep well plate 20 . Optionally, the accommodating table 11 may include one or more accommodating parts, and each accommodating part is used to accommodate a deep-well plate 20 . In some cases, the samples in the deep-well plate 20 on different receptacles may be different, so as to realize different nucleic acid extraction operations at the same time.

A limiting block 111 and an elastic piece 112 may be provided on each accommodating portion, and the deep hole plate 20 can be fixed on the accommodating portion through the cooperation of the limiting block 111 and the elastic piece 112 . The limit blocks 111 of two adjacent accommodating parts can be connected together, for example, can be integrally formed, which can increase the firmness of the limit blocks 111 and utilize the space more rationally.

A sample label 21 is provided on the side of the deep well plate 20 . Generally speaking, the deep well plate 20 will not be reused, so the sample label 21 can be adhered to the side of the deep well plate 20 in a sticking manner. Of course, in some cases, if it is considered that the deep well plate 20 may be reused, the sample label 21 can be fixed on the side of the deep well plate 20 in a detachable manner. The label 21 can be snapped into the card slot and fixed.

The sample label 21 on the side of the deep well plate 20 is used to indicate the information of the sample associated with the deep well plate 20. In actual use, the deep well plate 20 can be loaded with corresponding samples or sample reagents, and the sample label 21 can indicate the loaded The sample information of the sample, for example, the sample information can be the name of the sample, the serial number of the sample, and so on.

The first position sensor assembly is disposed on the accommodating table 11 for sensing whether the deep well plate 20 is accommodated in place. In the case where the accommodating table 11 includes one or more accommodating parts, a first position sensor assembly can be provided for each accommodating part, so that each first position sensor assembly can sense whether there is a The deep well plate 20 is held in place. The bottom of each accommodating portion may be provided with a heating device 50,

In a preferred manner, as shown in FIG. 2 , the first position sensor assembly includes two first position sensors 113 , and the two first position sensors 113 are respectively disposed at diagonal positions of the accommodating portion, for example, they may be respectively located at Next to two non-adjacent corners of the intermediate heating device 50 on the bottom of the accommodating portion. When the deep well plate 20 is accommodated in place, the two position sensor hoes will be displaced, trigger the switch, and generate a sensing signal indicating that the deep well plate 20 is placed in place, and the sensing signal can be transmitted to the control so that the controller controls the camera 30 to capture images when it is determined that the first position sensor assembly senses that the deep well plate 20 is accommodated in place.

In this way, it can be ensured that the deep well plate 20 is accommodated flat and in place, and the problem of one side being in place and the other side being lifted can be avoided, so that the sample label 21 can be aligned with the light inlet of the camera 30 to facilitate subsequent image acquisition. with identification.

Of course, the first position sensor assembly may also include more first position sensors 113, such as three, etc., which is not specifically limited. It can be understood that, in addition to the above-mentioned first position sensor 113 , the first position sensor assembly may also include a device for transmitting a sensing signal to the controller, such as an electronic circuit or a wireless transmission module, which is not specifically limited.

The first position sensor assembly senses that the deep well plate 20 is accommodated in place. Further, all the first position sensors 113 included in the first position sensor assembly generate sensing signals. Correspondingly, the controller determines that the first position sensor assembly senses that the deep well plate 20 is accommodated in place, and further comprises: receiving the sensing signals sent by all the first position sensors 113 included in the first position sensor assembly.

The camera 30 is used to capture images containing the sample labels 21 . In the case where the accommodating table 11 includes one or more accommodating parts, a camera 30 can be provided on one side of each accommodating part, and the position of the camera 30 can make the sample label 21 align with the camera when the deep-well plate 20 is accommodated in place The light inlet of 30 , that is, the sample label 21 is within the field of view of the camera 30 .

In actual work, nucleic acid extraction operations for samples in the deep-well plate 20 on different accommodating parts can be performed independently. For example, for each accommodating part, after placing the deep-well plate 20 in the accommodating part, the All the first position sensors 113 included in the first position sensor assembly on the accommodating part will generate sensing signals and transmit the sensing signals to the controller, and the controller will send the acquisition control signal to the camera 30 corresponding to the accommodating part, The camera 30 corresponding to the accommodating portion is controlled to capture images, so as to capture the image of the sample label 21 located on the deep-well plate 20 of the accommodating portion.

When the camera 30 receives the capture control signal sent by the controller, it can capture one or more frames of images, which is not specifically limited.

The label identification device 31 is connected with the camera 30 and the controller, and the camera 30 can transmit the image collected each time to the label identification device 31 . The label recognition device 31 receives the image and recognizes the sample label 21 in the image. Optionally, the label identification device 31 may be a two-dimensional code scanning device, the sample label 21 may be a two-dimensional code label, and a two-dimensional code identification method may be used to identify the sample label 21 in the image.

Optionally, the label identification device 31 and the camera 30 may be connected through a cable 32 . Preferably, the cable 32 here can be an optical fiber, and the transmission rate is faster. Of course, it is only preferred here, and is not specifically limited. Two sets of label identification devices 31 and cameras 30 may be provided on the nucleic acid extractor, and the two sets of label identification devices 31 and cameras 30 may share the same controller, or may use different controllers, which are not specifically limited.

The label recognition device 31 can obtain a recognition result when the recognition is successful, and the recognition result can include the information recognized from the sample label 21, such as sample information, and can send the recognition result to the controller. When the recognition fails, the above-mentioned recognition cannot be obtained. result.

The controller may receive the identification result, determine the type of the sample based on the identification result, and obtain a target nucleic acid extraction program corresponding to the category of the sample from a plurality of stored nucleic acid extraction procedures, and the target nucleic acid extraction procedure is used to realize nucleic acid extraction of the sample.

The differences between different nucleic acid extraction procedures can be reflected in the process, the time of each process, etc. For example, FFPE (formalin fixation and paraffin embedding, formalin fixation and paraffin embedding) procedure and cfDNA (free DNA fragment) procedure are two different nucleic acid extraction procedures, and the procedures are different. According to customer needs, The timing of each of these processes can be changed, and many other nucleic acid extraction procedures can be derived.

Multiple different nucleic acid extraction programs can be stored in the controller in advance, or stored in other devices, and can be called when needed. When saving, the type of the sample can be stored corresponding to the nucleic acid extraction procedure, so that the corresponding nucleic acid extraction procedure can be found according to the type of the sample.

After receiving the identification result, the controller can determine the category of the sample. The identification result can include the sample name, for example, the category of the sample can be determined according to the sample name, and then the determined category of the sample can be obtained from all stored nucleic acid extraction programs. For the corresponding target nucleic acid extraction program, the nucleic acid extraction of the sample can be realized by running the target nucleic acid extraction program.

In the above-mentioned embodiment, the sample label 21 is provided on the side of the deep well plate 20, and by arranging the first position sensor assembly on the accommodating table 11, it can be sensed whether the deep well plate 20 is accommodated in place, so that the deep well plate 20 can be The sample label 21 of 20 can be aligned with the light inlet of the camera 30, so that the controller can accurately control the timing of the camera 30 to collect images, and the camera 30 can collect the image including the complete sample label 21, so as to avoid collecting only the partial sample label 21. The image leads to the problem of subsequent inability to identify, and the size of the camera 30 is smaller than that of the label recognition device 31, and it is easier to be set to the position of the sample label 21 aligned with the deep well plate 20. Therefore, the camera 30 is used to capture The image and label identification device 31 identify the method, the label identification device 31 can be set to the spare position of the nucleic acid extraction instrument, which can be more conducive to the miniaturization of the entire nucleic acid extraction instrument, and the label identification device 31 can identify the sample label from the image. The identification result of 21 can then be sent to the controller, and the controller can determine the required target nucleic acid extraction procedure according to the identification result, and the target nucleic acid extraction procedure can realize the nucleic acid extraction of the sample. Manually recording sample information and selecting nucleic acid extraction procedures can reduce labor and time costs, avoid manual errors, and improve the accuracy of nucleic acid extraction.

In addition, the samples are first classified, in which samples with the same nucleic acid extraction process can be classified into one category, and the corresponding nucleic acid extraction procedures are searched according to the category, compared to the corresponding nucleic acid extraction procedures according to the name or number of the sample. In other words, the number of nucleic acid extraction procedures that need to be preserved can be reduced.

In one embodiment, on the basis of the foregoing embodiments, the nucleic acid extractor may further include a movable mechanism (not marked in the figure), the movable mechanism is installed on the bracket 10, and the accommodating table 11 is installed on the movable mechanism, And can move back and forth relative to the camera 30 through a movable mechanism.

The movable device may include, for example, a slide rail provided on the accommodating table 11 or the accommodating portion, and a chute provided on the bracket 10 , and the accommodating table 11 can be realized through the cooperation of the slide rail and the chute.

During the process of the accommodating table 11 moving back and forth, the sample label 21 is always aligned with the light inlet of the camera 30 . In other words, the forward and backward movement here is limited to a linear movement close to the camera 30 or away from the camera 30 , so as to prevent the sample label 21 from moving out of the field of view of the camera 30 .

In this embodiment, through the movable mechanism, the accommodating table 11 can be moved back and forth relative to the camera 30, so that the distance between the sample label 21 on the deep well plate 20 and the camera 30 can be adjusted, that is, the depth of field of the camera 30 can be adjusted, It can greatly improve the accuracy of label recognition.

In one embodiment, on the basis of the foregoing embodiments, the controller is further configured to: control the camera 30 to capture an image when it is determined that the first position sensor assembly senses that the deep well plate 20 is accommodated in place, and if the following If the recognition result is not received within a set period of time, the movable mechanism is controlled to drive the receiving table 11 to move relative to the camera 30 to adjust the distance between the sample label 21 and the camera 30. When the moving of the receiving table 11 is completed, the camera 30 is controlled to collect new data. image;

The label identification device 31 is further configured to: identify the sample label 21 in the image each time an image is received, and send the identification result to the controller when the identification result is obtained.

The controller determines that the first position sensor assembly senses that the deep well plate 20 is accommodated in place, and can only ensure that the sample label 21 is aligned with the light inlet of the camera 30, but cannot guarantee that the sample label 21 device can collect images from the camera 30 When the sample label 21 is identified, the sample label 21 cannot be clearly imaged on the camera 30 because the distance between the sample label 21 and the camera 30 may not match the focal length of the camera 30 .

Therefore, in this embodiment, after the controller controls the camera 30 to capture the image, the camera 30 sends the image to the label recognition device 31, and the label recognition device 31 recognizes the sample label 21 in the image, and only sends the image when the recognition is successful. The identification result is sent to the controller, otherwise it is not sent. If the controller does not receive the recognition result within a set period of time after controlling the camera 30 to capture the image, it means that the label recognition device 31 cannot recognize the sample label 21, and at this time, the controller controls the movable mechanism to drive the accommodating table 11 to face the camera 30. Move, so as to adjust the distance between the sample label 21 and the camera 30, each time you can move a certain distance, when the movement is completed, control the camera 30 again to collect a new image, and the camera 30 will send the collected image to the label. The identification device 31 recognizes again. If the label identification device 31 recognizes successfully, the identification result can be sent to the controller, otherwise it is not sent. If the controller does not receive the identification result again within a set period of time, it can control the movable mechanism again. The accommodating table 11 is driven to move relative to the camera 30 until the recognition result is obtained.

In this embodiment, the controller can control the movable mechanism to drive the accommodating table 11 to move relative to the camera 30 according to whether the label recognition device 31 feeds back the recognition result within a set period of time, so as to adjust the distance between the sample label 21 and the camera 30 , that is, The depth of field of the camera 30 is adjusted until the label identification device 31 can obtain the identification result, which further ensures the automation and accuracy of the identification of the sample label 21 .

In one embodiment, when the controller controls the movable mechanism to drive the accommodating table 11 to move relative to the camera 30, it is further used for:

Check whether the current position of the accommodating table 11 is the set position, or whether the direction from the set position to the current position is the first direction, and the first direction is the direction away from the camera 30 or the direction close to the camera 30;

If so, when the distance between the current position and the set position is greater than or equal to the set distance, after the control accommodating table 11 is reset to the set position, it moves the specified distance in the second direction, and the second direction is the opposite direction of the first direction, The specified distance is less than the set distance; when the distance between the current position and the set position is less than the set distance, the accommodating table 11 is controlled to move the specified distance in the first direction;

If not, when the distance between the current position and the set position is greater than or equal to the set distance, the accommodating table 11 is controlled to move the specified distance in the first direction after being reset to the set position; when the distance between the current position and the set position is less than When the distance is set, the accommodating table 11 is controlled to move the specified distance in the second direction.

The set position can be the initial position of the accommodating table 11 , which can be set according to experience, for example, the position where the accommodating table 11 is located when most of the sample labels 21 can be identified is used as the set position. Optionally, after receiving the identification result of the label identification device 31, the controller can modify the set position to the current position of the accommodating table 11 as the positioning of the accommodating table 11 in the next nucleic acid extraction, which can improve the scanning efficiency. Of course, the position of the accommodating table 11 can be re-adjusted based on the set position in the next nucleic acid extraction.

In this embodiment, when the controller controls the movable mechanism to drive the accommodating table 11 to move, it can move in the same direction first. If the distance from the set distance is too far after several movements, the label identification device 31 still cannot recognize it. The sample label 21 is moved in the opposite direction, thereby ensuring that the label identification device 31 can finally identify the sample label 21, and the identification efficiency is high.

In one embodiment, continue referring to FIG. 1 and FIG. 2 , on the basis of the foregoing embodiments, the nucleic acid extractor may further include a magnetic rod holder 40 and a second position sensor assembly 41 .

The magnetic rod sleeve 40 can be installed on the bracket 10, and specifically can be located above the accommodating table 11. Of course, a space for the deep hole plate 20 needs to be left between the magnetic rod sleeve 40 and the accommodating table 11. The magnetic rod sleeve 40 is used for for accommodating the magnetic rod sleeve 42 . The second position sensor assembly 41 is disposed on the magnetic rod sleeve holder 40 for sensing whether the magnetic rod sleeve 42 is accommodated in place.

The controller is further configured to: run the target nucleic acid extraction program when it is determined that the second position sensor assembly 41 senses that the magnetic rod cover 42 is accommodated in place, otherwise prohibit running the target nucleic acid extraction program.

In this embodiment, by arranging the second position sensor assembly 41 on the magnetic rod cover frame 40, it can be sensed whether the magnetic rod cover 42 is accommodated in place, and the target nucleic acid extraction program will be executed only when it is accommodated in place, Otherwise, it is forbidden to run the target nucleic acid extraction program, thus reducing the risk of experiment failure and instrument damage caused by leakage of the magnetic rod sleeve 42 during the nucleic acid extraction experiment.

In one embodiment, the magnet bar sleeve holder 40 includes N magnet bar sleeve accommodating portions, each of which is used for accommodating a row of magnet bar sleeves 42 , and N is greater than or equal to 1. In FIG. 1 , the shelf above the magnet bar holder 40 can be used for fixing the magnet bars (not marked in the figure).

Correspondingly, the second position sensor assembly 41 includes N second position sensors, which are respectively disposed at the designated positions of the N magnetic rod sleeve accommodating parts. The second position sensor assembly 41 senses that the magnetic rod sleeve 42 is accommodated in place. Further, the N second position sensors all sense that the magnetic rod sleeve 42 reaches the designated position of the magnetic rod sleeve accommodating part.

When each second position sensor senses that the magnetic rod sleeve 42 reaches the designated position of the corresponding magnetic rod sleeve accommodating part, it can generate a sensing signal for indicating that the magnetic rod sleeve 42 is installed in place. The target nucleic acid extraction procedure will not be run until the sensing signals sent by all the second sensors included in the second position sensor assembly 41 .

As shown in FIG. 1 and FIG. 2 , the magnetic rod sleeve frame 40 includes four magnetic rod sleeve accommodating portions, which can accommodate four rows of magnetic rod sleeves 42 . Correspondingly, the four second position sensors can be respectively arranged in the four magnetic rod sleeves 42 . The target nucleic acid extraction program will be executed only when all the four rows of magnetic rod sleeves 42 are accurately accommodated in the four magnetic rod sleeve accommodating parts at the designated positions of the magnetic rod sleeve accommodating parts.

Referring to FIG. 2 , a heating device 50 may also be provided on the nucleic acid extractor, and the heating device 50 may be used to heat the reagents in the deep well plate 20 .

The heating device 50 can be fixed on the bottom of the accommodating table 11. In the case that the accommodating table 11 includes a plurality of accommodating parts, one or more heating devices 50 can be arranged on each accommodating part, as shown in FIG. 2 , Three heating devices 50 can be arranged on each accommodating part, and the three heating devices 50 can share the same cooling fan 54 , and the cooling fan 54 is arranged at the bottom of the three heating devices 50 . The number of heating bar assemblies in the three heating devices 50 may not be limited to one. In FIG. 2 , at the bottom of the first accommodating portion on the left side, the heating bar assemblies included in the three heating devices 50 are 2, 3 and 1, the number of heating bar assemblies of each heating device 50 can be adjusted according to the reagent row that needs to be heated in the deep well plate 20, so that when the maximum continuous row that needs to be heated is 3, the heating bar assemblies included in the corresponding heating device 50 The number is 3, and there is no specific limit.

3 and 4, the heating device 50 may include a heating bar assembly, a heat shield, a heat insulating bead 52, and a cooling fan (the cooling fan 54 is shown in FIG. 5, not shown in FIGS. 3 and 4).

The heating bar assembly may include a heating bar body 531 and a heating head 532 , and the heating head 532 is located at the upper end of the heating bar body 531 . In this embodiment, the upper end of the heating strip body 531 refers to the end closer to the deep well plate 20 after being installed in the nucleic acid extractor.

The upper end surface of the heating head 532 may be in contact with the bottom of the deep well plate 20 to transfer the heat of the heating bar body 531 to the deep well plate 20 . The bottom of the deep hole plate 20 may have bumps. In this embodiment, the upper end surface of the heating head 532 may have a dimple 5321. Better contact of the orifice plate 20.

Of course, the heating bar assembly may also include other components, such as a heating element 533, and a cavity is provided in the heating bar body 531 (the cavity can be opened or closed through a cover body, the cover body is not shown in the figure) , the heating element 533 is built in the cavity, and is connected to an external power supply and a control device through a circuit, wherein the circuit can pass through the heating strip body 531 and the heat shield. Optionally, the heating element 533 may include a heating plate, a temperature sensor, etc., which is not specifically limited.

The heat shield has an upper end opening 511 and a lower end opening (the lower end opening is not marked in the figure), the heat shield is sleeved on the heating bar assembly and protrudes from the upper end opening 511 by the heating head 532, and the lower end opening can be used to place the heating bar assembly. into the heat shield.

The upper end opening 511 is located at the upper end of the heat shield, and the lower end opening may be located at the lower end of the heat shield, and the lower end may be the end opposite to the upper end. The size of the lower end opening can be larger than that of the upper end opening 511 , so that the heating bar assembly can enter from the lower end opening and cannot exit from the upper end opening 511 , of course, the upper end opening 511 is sufficient for the heating head 532 to protrude.

The heat insulating bead 52 seals the lower end opening of the heat shield to form a fully enclosed space between the heat insulating bead 52, the heat shield and the heating head 532, and the heating bar body 531 is enclosed in the totally enclosed space.

Optionally, the heat insulating pressure strip 52 may be fixed to the lower end opening of the heat shield by means of snap-fastening, and seal the lower end opening. Of course, the fixing method of the heat insulating bead 52 and the heat shield is not limited to this, for example, the connection and fixation can also be realized by an external connection member.

The full enclosing space means that the upper, lower, front, left, and right ends of the heating head 532 have blocking walls, wherein the blocking walls at the upper end can be composed of the heating head 532 and part of the wall of the heat shield, and the blocking walls at the front, rear, left, and right ends can be formed by the four sides of the heat shield. The barrier wall at the lower end can be composed of thermal insulation strips 52 .

The heat shield and/or the heat insulating layer 52 are provided with heat dissipation holes, and the number and specific positions of the heat dissipation holes are not limited.

Preferably, two sides of the heat shield are respectively provided with a row of first heat dissipation holes 512 , and a row of second heat dissipation holes 521 are provided on the heat insulation bead 52 . Wherein, the two sides of the heat shield may be two sides with larger areas, and the row of second heat dissipation holes 521 on the heat insulating bead 52 may be arranged in the length direction of the heat insulating bead 52 . It can be understood that the above method is only a preferred method, and is not limited to this. For example, multiple rows of heat dissipation holes may be opened on both sides of the heat shield, or only one row or more rows may be opened on one side of the heat shield. For the heat dissipation holes, multiple rows of heat dissipation holes may also be opened on the heat insulating layer 52, which is not limited in detail.

The number of cooling holes in a row is multiple. The size of each heat dissipation hole is much smaller than the upper end opening 511 or the lower end opening. For example, the diameter of each heat dissipation hole may be in the order of millimeters, for example, the diameter may be less than 5mm. Of course, the specific size is not limited.

The heat dissipation fan 54 is disposed toward at least one heat dissipation hole. Preferably, the cooling fan 54 is disposed at the bottom of the heat insulating bead 52 and faces the second heat dissipation hole 521 of the heat insulating bead 52 , which facilitates the installation of the heat dissipation fan 54 and facilitates the use of the same heat dissipation fan by multiple heating devices 50 . 54. Of course, the position of the heat dissipation fan 54 is not limited to this, for example, it can also be disposed on one side of the heat shield and face the first heat dissipation hole 512 of the heat shield.

The cooling fan 54 may not supply air when the heating device 50 is heating, and start supplying air when the heating device 50 finishes heating.

In the above-mentioned embodiment, since a fully enclosed space is formed between the heat insulation layer 52, the heat shield and the heating head 532, the heating strip body 531 is enclosed in the fully enclosed space, and the cooling fan 54 may not work during heating In this way, the air in the fully enclosed space is still, and the thermal conductivity of the static air is extremely low. Even if there are heat dissipation holes on the heat shield and/or the heat insulation layer 52, it is not easy to dissipate heat through the heat dissipation holes. Compared with the heating strip body 531 For the half-enclosed method, the fully enclosed thermal insulation can make heating and thermal insulation better, and can more quickly and effectively heat the reagent to be heated to the required temperature, improving heating efficiency, especially for large-volume or demanding The reaction reagent with higher temperature has better effect; and when heat dissipation is required, the cooling fan 54 can be controlled to work, and air is supplied to the interior of the fully enclosed space through the cooling holes, so that gas convection is generated inside the fully enclosed space, so that convection can be carried out through the cooling holes. Heat dissipation, the heat dissipation effect is also very good.

In addition, generally speaking, a plurality of heating devices 50 will be installed on the nucleic acid extractor. In the embodiment of the present invention, the method of fully surrounding heat insulation greatly limits the heat dissipation of the heating strip body 531 during the heating process, and greatly reduces the heat dissipation. Interaction between adjacent heating bar bodies 531 .

Optionally, there is a gap between at least one side surface of the heating strip body 531 and a corresponding side wall of the heat shield, and the at least one side surface here may refer to a side surface with heat dissipation holes. In this way, during heat dissipation, convection can be formed in the space of the gap, so that the heat dissipation effect is better.

Optionally, a gap exists between the bottom of the heating bar body 531 and the heat insulating bead 52 . In the same way, it is beneficial to form convection in the fully enclosed space during heat dissipation, so as to facilitate heat dissipation.

Preferably, referring to FIG. 3 and FIG. 4 , there are gaps between the two sides of the heat shield with the first heat dissipation holes 512 and the heating bar body 531 , and the bottom of the heating bar body 531 and the heat insulating bead 52 also have gaps. There is a gap, so that when the cooling fan 54 supplies air to the second heat dissipation hole 521 of the heat insulation bead 52, convection is formed in the space in the fully enclosed space, and the air flow carries heat from the first heat dissipation hole 512 of the heat shield. Go out, the cooling effect is better.

Optionally, referring to FIG. 3 , the bottom of the heating bar body 531 is further provided with cooling ribs 534 , which can be arranged in the length direction of the heating bar body 531 . The heat dissipation rib 534 can be arranged in the gap between the heating bar body 531 and the heat insulating bead 52. Optionally, the heat dissipation rib 534 can be in contact with the heat insulating bead 52, so that the heat insulating bead 52 can press the entire heating bar assembly against the heat-insulating bead 52. in the heat shield.

Due to the existence of the heat dissipation ribs 534, the heat dissipation area of the heating strip body 531 is larger, and when the airflow passes through, more heat can be taken away, and the heat dissipation effect is better.

Preferably, the heat dissipation rib 534 and the heating strip body 531 are integrated. For example, the heat dissipation rib 534 and the heating strip body 531 can be integrally formed, and can be made of the same mold and the same material. material or an alloy material containing any of these elements, etc.

Optionally, referring to FIG. 3 and FIG. 4 , the size of the upper end opening 511 of the heat shield matches the size of the heating head 532 , so that the heating head 532 can seal the upper end opening 511 of the heat shield. In this way, heat dissipation from the upper end opening 511 of the heat shield can be avoided to ensure heat dissipation efficiency.

Optionally, referring to FIG. 3 , the heating strip body 531 and the heating head 532 have an integral structure, and they are both made of metal materials, and specifically can be any material such as copper, aluminum, iron, etc. Alloy materials of any element, etc. In one example, the heating strip body 531 , the heating head 532 and the heat dissipation ribs 534 can be integrally formed.

Alternatively, the heating strip body 531 and the heating head 532 may also be separate structures, and the separate structures will be described in more detail below.

The heating strip body 531 is made of metal material, and the heating head 532 is made of flexible thermally conductive material. The first surface of the heating head 532 is attached to the heating strip body 531, and the second surface of the heating head 532 can be attached to the bottom of the deep well plate 20, so as to transfer the heat of the heating strip body 531 to the deep well plate 20, the first The surface and the second surface are two different surfaces of the heating head 532 .

The second surface is, for example, the upper end surface of the heating head 532 , specifically, the surface of the heating head 532 facing the deep well plate 20 when the second heating device 50 is installed on the nucleic acid extractor. The first surface is, for example, the lower end surface of the heating head 532 , specifically, the end surface opposite to the upper end surface of the heating head 532 .

Since the heating strip body 531 and the heating head 532 are separate structures, and the heating head 532 is made of a flexible thermally conductive material, due to its flexibility, the heating head 532 can fit well with the heating strip body 531 on the one hand, and the other On the one hand, it can fit well with the deep hole plate 20 , avoid poor contact with the deep hole plate 20 to generate a gap, and can play a better heat conduction effect between the heating strip body 531 and the deep hole plate 20 .

Optionally, the heating head 532 and the heating strip body 531 are fixed to each other in a nested manner, which can make the contact between the two more comprehensive, and avoid the problem of poor contact caused by the action of the external connector.

Of course, the specific fixing method of the heating head 532 and the heating strip body 531 is not limited to this, and other fixing methods that can make there no gap between the heating head 532 and the heating strip body 531 are also applicable.

Optionally, the heating head 532 is made of thermally conductive silicone rubber, that is, the flexible thermally conductive material is thermally conductive silicone rubber. Thermally conductive silicone rubber is a non-fluid soft material, which is different from a fluid soft material - thermally conductive silicone grease.

In this embodiment, the heating head 532 is made of thermally conductive silicone rubber, and then sleeved on the heating strip body 531. It has a certain degree of elasticity, can be compressed after being stressed, and can be restored after the force is removed. The original shape, this flexibility can make the heating head 532 fit more closely with the heating strip body 531 and the deep hole plate 20 .

Embodiments of the present invention further provide a nucleic acid extraction method, which is applied to the controller in the nucleic acid extraction apparatus described in the foregoing embodiments. In other words, the execution subject of the nucleic acid extraction method is the controller. Referring to Figure 6, the method includes:

S100: When it is determined that the first position sensor assembly senses that the deep-well plate is accommodated in place, control the camera to capture an image; wherein, the label recognition device can be When the image is displayed, the sample label in the image is recognized, and the recognition result is sent to the controller when the recognition result is obtained;

S200: Check whether an identification result is received within a set period of time thereafter;

S300: If not, control the accommodating table to move relative to the camera to adjust the distance between the sample label and the camera, and when the accommodating table is moved, control the camera to collect a new image, and return to The step of checking whether the recognition result is received within a set period of time afterward;

S400: If yes, determine the category of the sample based on the identification result, and obtain a target nucleic acid extraction program corresponding to the category of the sample from a plurality of stored nucleic acid extraction programs, where the target nucleic acid extraction program is used to achieve all Nucleic acid extraction from the samples.

In one embodiment, the controlling the accommodating platform to move relative to the camera includes:

Check whether the current position of the accommodating table is the set position, or whether the direction from the set position to the current position is the first direction, and the first direction is the direction away from the camera or the direction close to the camera;

If so, when the distance between the current position and the set position is greater than or equal to the set distance, the accommodating table is controlled to be reset to the set position and then moved to the second direction by a specified distance, and the second direction is the set position. In the opposite direction of the first direction, the specified distance is less than the set distance; when the distance between the current position and the set position is less than the set distance, the accommodating table is controlled to move the specified distance in the first direction;

If not, when the distance between the current position and the set position is greater than or equal to the set distance, the accommodating table is controlled to be reset to the set position and then moved to the first direction by the specified distance; When the distance of the fixed position is less than the set distance, the accommodating table is controlled to move the specified distance in the second direction.

In one embodiment, after obtaining the target nucleic acid extraction procedure corresponding to the category of the sample from a plurality of stored nucleic acid extraction procedures, the method further includes:

When it is determined that the second position sensor assembly senses that the magnetic rod cover is accommodated in place, the target nucleic acid extraction program is executed, otherwise the target nucleic acid extraction program is prohibited from running, and the second position sensor assembly is disposed on the magnetic rod. On the sleeve frame, the magnetic rod sleeve frame is used for accommodating the magnetic rod sleeve.

For the content of the nucleic acid extraction method of the present invention, reference may be made to the descriptions in the foregoing embodiments, and the same or similar points will not be repeated here.

The present invention also provides a controller, including a processor and a memory; the memory stores a program that can be called by the processor; wherein, when the processor executes the program, the nucleic acid as described in the foregoing embodiments is implemented Extraction Method.

The present invention also provides a machine-readable storage medium on which a program is stored, and when the program is executed by a processor, implements the nucleic acid extraction method described in the foregoing embodiments.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: The technical solutions described in the foregoing embodiments can still be modified, or some or all of the technical features thereof can be equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the embodiments of the present invention. scope.

Claims (17)

1. A nucleic acid extractor, comprising: the device comprises a support, a containing table, a first position sensor assembly, a camera, a label identification device and a controller;

the containing table is arranged on the bracket and used for containing the deep hole plate; a sample label is arranged on the side surface of the deep hole plate and is used for indicating information of a sample associated with the deep hole plate;

the first position sensor assembly is arranged on the accommodating table and used for sensing whether the deep hole plate is accommodated in place or not;

the camera is arranged on the bracket, the sample label is aligned to a light inlet of the camera when the deep hole plate is accommodated in place, and the camera is used for collecting images containing the sample label;

the label identification device is connected with the camera and used for receiving the image and identifying the sample label in the image;

the controller is connected with the label identification device and used for receiving an identification result, determining the category of the sample based on the identification result, and acquiring a target nucleic acid extraction program corresponding to the category of the sample from a plurality of stored nucleic acid extraction programs, wherein the target nucleic acid extraction program is used for realizing nucleic acid extraction of the sample.

2. The nucleic acid extractor of claim 1, further comprising a movable mechanism;

the movable mechanism is mounted on the bracket;

the containing table is mounted on the movable mechanism and can move back and forth relative to the camera through the movable mechanism; and in the process of moving the containing table back and forth, the sample label is always aligned with the light inlet of the camera.

3. The nucleic acid extractor of claim 2,

the controller is further configured to: when the first position sensor assembly is determined to sense that the deep hole plate is accommodated in place, controlling the camera to acquire an image, if an identification result is not received within a set period of time, controlling the movable mechanism to drive the accommodating table to move relative to the camera so as to adjust the distance between the sample label and the camera, and controlling the camera to acquire a new image when the accommodating table is moved;

the tag identification device is further configured to: and identifying the sample label in the image every time the image is received, and sending an identification result to the controller when the identification result is obtained.

4. The nucleic acid extractor of claim 3, wherein the controller controls the movable mechanism to drive the holding table to move relative to the camera, and further:

checking whether the current position of the accommodating table is a set position or whether the direction from the set position to the current position is a first direction, wherein the first direction is a direction far away from the camera or a direction close to the camera;

if so, controlling the accommodating table to move to a specified distance in a second direction after the accommodating table is reset to the set position when the distance between the current position and the set position is greater than or equal to the set distance, wherein the second direction is the opposite direction of the first direction, and the specified distance is smaller than the set distance; when the distance between the current position and the set position is smaller than the set distance, controlling the accommodating table to move to the first direction by a designated distance;

if not, when the distance between the current position and the set position is greater than or equal to the set distance, controlling the accommodating table to move to the first direction for a specified distance after being reset to the set position; and when the distance between the current position and the set position is smaller than the set distance, controlling the accommodating table to move to the second direction by a designated distance.

5. The nucleic acid extractor of claim 1, further comprising a magnetic rod holder and a second position sensor assembly;

the magnetic bar sleeve frame is used for accommodating the magnetic bar sleeve;

the second position sensor assembly is arranged on the magnetic bar sleeve frame and used for sensing whether the magnetic bar sleeve is accommodated in place or not;

the controller is further configured to: and when the second position sensor assembly is determined to sense that the magnetic rod sleeve is accommodated in place, operating the target nucleic acid extraction program, and otherwise, inhibiting the target nucleic acid extraction program from being operated.

6. The nucleic acid extractor of claim 5, wherein,

the magnetic rod sleeve frame comprises N magnetic rod sleeve accommodating parts, each magnetic rod sleeve accommodating part is used for accommodating a row of magnetic rod sleeves, and N is greater than or equal to 1;

the second position sensor assembly comprises N second position sensors which are respectively arranged at the appointed positions of the N magnetic rod sleeve accommodating parts;

the second position sensor assembly sensing that the magnetic rod sleeve is accommodated in place further comprises: and the N second position sensors sense that the magnetic rod sleeve reaches the specified position of the magnetic rod sleeve accommodating part.

7. The nucleic acid extractor of claim 1,

the accommodating table at least comprises one accommodating part, and each accommodating part is used for accommodating one deep hole plate;

the first position sensor assembly includes at least two first position sensors, wherein the two first position sensors are respectively disposed at diagonal positions of the accommodating portion.

8. The nucleic acid extractor according to claim 1, further comprising a heating device fixed to a bottom of the mounting table for heating the deep hole plate;

the heating device comprises a heating strip assembly, a heat shield, a heat insulation pressing strip and a cooling fan;

the heating strip assembly comprises a heating strip body and a heating head, and the heating head is positioned at the upper end of the heating strip body;

said heat shield having an upper end opening and a lower end opening, said heat shield being mounted over said heating bar assembly and extending from said upper end opening by said heating tip;

the heat insulation pressing strip seals the lower end opening of the heat insulation cover so as to form a fully-enclosed space among the heat insulation pressing strip, the heat insulation cover and the heating head, and the heating strip body is enclosed in the fully-enclosed space;

the heat shield and/or the heat-insulating pressing strip are/is provided with heat dissipation holes, and the heat dissipation fan is arranged towards at least one heat dissipation hole.

9. The nucleic acid extractor of claim 8,

at least one row of heat dissipation holes are formed in at least one side of the heat shield;

at least one row of heat dissipation holes are formed in the heat insulation pressing strip.

10. The nucleic acid extractor of claim 9, wherein,

the heat dissipation fan is arranged at the bottom of the heat insulation pressing strip and faces the heat dissipation holes of the heat insulation pressing strip.

11. The nucleic acid extractor of claim 8,

a gap exists between at least one side of the heating strip body and a corresponding side wall of the heat shield;

a gap exists between the bottom of the heating strip body and the heat insulation pressing strip.

12. The nucleic acid extractor of claim 11,

the bottom of the heating strip body is also provided with a heat dissipation rib.

13. The nucleic acid extractor of claim 8,

the size of the upper end opening of the heat shield is matched with the size of the heating head, so that the heating head can seal the upper end opening of the heat shield.

14. The nucleic acid extractor of claim 8,

the heat radiation fan is used for: and when the heating device is heated, air is not supplied, and when the heating device finishes heating, air supply is started.

15. A method for extracting nucleic acid, which is applied to a controller in the nucleic acid extracting apparatus according to any one of claims 1 to 15, the method comprising:

when the first position sensor assembly is determined to sense that the deep hole plate is accommodated in place, controlling the camera to collect images; the label identification device can identify a sample label in an image when receiving the image collected by the camera each time, and send an identification result to the controller when obtaining the identification result;

checking whether an identification result is received within a set time length;

if not, controlling the accommodating table to move relative to the camera to adjust the distance between the sample label and the camera, controlling the camera to acquire a new image when the accommodating table is moved, and returning to the step of checking whether an identification result is received within a set period of time;

and if so, determining the type of the sample based on the identification result, and acquiring a target nucleic acid extraction program corresponding to the type of the sample from a plurality of stored nucleic acid extraction programs, wherein the target nucleic acid extraction program is used for realizing the nucleic acid extraction of the sample.

16. The method for extracting nucleic acid according to claim 15, wherein the controlling the accommodating stage to move relative to the camera includes:

checking whether the current position of the accommodating table is a set position or whether the direction from the set position to the current position is a first direction, wherein the first direction is a direction far away from the camera or a direction close to the camera;

if so, controlling the accommodating table to move to a specified distance in a second direction after the accommodating table is reset to the set position when the distance between the current position and the set position is greater than or equal to the set distance, wherein the second direction is the opposite direction of the first direction, and the specified distance is smaller than the set distance; when the distance between the current position and the set position is smaller than the set distance, controlling the accommodating table to move to the first direction by a designated distance;

if not, when the distance between the current position and the set position is greater than or equal to the set distance, controlling the accommodating table to move to the first direction for a specified distance after being reset to the set position; and when the distance between the current position and the set position is smaller than the set distance, controlling the accommodating table to move to the second direction by a designated distance.

17. The method for extracting nucleic acid according to claim 15, wherein after obtaining the target nucleic acid extraction program corresponding to the category of the sample from the plurality of stored nucleic acid extraction programs, the method further comprises:

and when determining that the second position sensor assembly senses that the magnetic rod sleeve is accommodated in place, operating the target nucleic acid extraction program, otherwise, prohibiting the target nucleic acid extraction program, wherein the second position sensor assembly is arranged on the magnetic rod sleeve frame, and the magnetic rod sleeve frame is used for accommodating the magnetic rod sleeve.

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