CN217438191U - Nucleic acid constant temperature amplification assembly and nucleic acid detection equipment - Google Patents
Nucleic acid constant temperature amplification assembly and nucleic acid detection equipment Download PDFInfo
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- CN217438191U CN217438191U CN202221324464.4U CN202221324464U CN217438191U CN 217438191 U CN217438191 U CN 217438191U CN 202221324464 U CN202221324464 U CN 202221324464U CN 217438191 U CN217438191 U CN 217438191U
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Abstract
The utility model discloses a nucleic acid constant temperature amplification subassembly and nucleic acid check out test set, wherein nucleic acid constant temperature amplification subassembly includes the bottom plate, be provided with first side post and second side post on the bottom plate, it places the district to be equipped with the sample between first side post and the second side post, the outside in district is placed to the sample is from last to having set gradually first heated board down, second heated board and third heated board, first heated board, second heated board and third heated board are all connected between first side post and second side post, be equipped with first semiconductor heating refrigeration piece on the first heated board, be equipped with second semiconductor heating refrigeration piece on the second heated board, be equipped with third semiconductor heating refrigeration piece on the third heated board. The utility model discloses the constant temperature of nucleic acid amplifys the subassembly and has avoided the sample in the risk of leaking of nucleic acid testing in-process, according to the difference of the required temperature of nucleic acid cracking reaction, cleaning process, amplification reaction, adopts semiconductor heating refrigeration piece to carry out independent temperature regulation, has shortened the nucleic acid testing cycle, has improved nucleic acid detection efficiency.
Description
Technical Field
The utility model relates to a nucleic acid detects technical field, concretely relates to nucleic acid constant temperature amplification subassembly and nucleic acid testing equipment.
Background
Nucleic acid detection is an important means for detecting and identifying microorganisms such as viruses and bacteria. In order to improve the detection sensitivity, the extraction, cleaning, washing and amplification of nucleic acid are required before the nucleic acid detection, most of the current commercial nucleic acid extraction and detection equipment types are open, nucleic acid cross contamination is easily caused, and safety risks to people also exist when pathogenic bacteria or viruses are detected; the time consumption of nucleic acid extraction and amplification is a main factor causing long nucleic acid detection period, and the temperature has a great influence on the nucleic acid extraction and amplification time. Therefore, there is a need to design a nucleic acid isothermal amplification module to solve the above existing technical problems.
SUMMERY OF THE UTILITY MODEL
In order to solve the above problem, the utility model provides a technical scheme does:
the utility model provides a nucleic acid constant temperature amplification subassembly, includes the bottom plate, be provided with first side post and second side post on the bottom plate, first side post with be equipped with the sample between the second side post and place the district, the outside in sample placement district is from last to having set gradually first heated board, second heated board and third heated board down, first heated board the second heated board with the third heated board is all connected first side post with between the second side post, be equipped with first semiconductor heating refrigeration piece on the first heated board, be equipped with second semiconductor heating refrigeration piece on the second heated board, be equipped with third semiconductor heating refrigeration piece on the third heated board.
The utility model discloses further set up to first heated board with be provided with first partition panel between the second heated board, the second heated board with be provided with the second partition panel between the third heated board.
The utility model discloses further set up to on the first side post with all be provided with boss and lower boss on the second side post, first heated board is fixed first side post go up on the boss with the second side post go up on the boss, the second heated board is fixed first side post down on the boss with the second side post down on the boss.
The utility model discloses further set up to third semiconductor heating refrigeration piece is located the bottom of third heated board.
The utility model discloses further set up to the fluorescence detection through-hole has been seted up to the side of third heated board.
The utility model discloses further set up to on the first heat preservation board or be provided with a temperature sensor on the first semiconductor heating refrigeration piece.
The utility model discloses further set up to on the second heated board or be provided with second temperature sensor on the second semiconductor heating refrigeration piece.
The utility model discloses further set up to be connected with a temperature protection switch on the first semiconductor heating refrigeration piece.
The utility model discloses further set up to be connected with second temperature protection switch on the second semiconductor heating refrigeration piece.
The utility model discloses further set up to the sample is placed the district and is kept away from the width of the one end of bottom plate is greater than the sample is placed the district and is close to the width of the one end of bottom plate.
A nucleic acid detection device comprises the nucleic acid isothermal amplification assembly.
Adopt the technical scheme provided by the utility model, compare with prior art, have following beneficial effect:
this technical scheme nucleic acid constant temperature amplification subassembly main part adopts first side post, second side post, first heated board, second heated board and third heated board to connect and forms, and the sample is placed the center that the district lies in nucleic acid constant temperature amplification subassembly main part, and concrete working method is: the reagent tube containing the sample to be detected is placed in the sample placing area, the first heat-insulating plate is heated to improve the nucleic acid extraction rate of the cracking area when the first semiconductor heating and refrigerating sheet works, the second heat-insulating plate is heated to shorten the cleaning time of the nucleic acid in the cleaning area when the second semiconductor heating and refrigerating sheet works, and the third semiconductor heating and refrigerating sheet is cooled to enable the reaction area to reach the optimal temperature for nucleic acid amplification, so that the time required by the nucleic acid amplification in the reaction area is shortened. The utility model discloses the district is all placed at the sample to nucleic acid amplification's whole reactions, has avoided the sample in nucleic acid amplification and testing process's the risk of leaking, places the district to the sample from last to carrying out the temperature subregion down, according to nucleic acid cracking reaction, cleaning process, the required temperature's of amplification reaction difference, adopts semiconductor heating refrigeration piece to carry out independent temperature regulation respectively, has shortened the nucleic acid testing cycle, has improved nucleic acid detection efficiency greatly.
Drawings
Fig. 1 is a front perspective view of an embodiment of the present invention.
Fig. 2 is a back perspective view of the embodiment of the present invention.
Fig. 3 is a longitudinal sectional view of the embodiment of the present invention.
Fig. 4 is a transverse sectional view of an embodiment of the present invention.
Detailed Description
For a further understanding of the present invention, reference will be made to the following detailed description taken in conjunction with the accompanying drawings and examples.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, integrally connected, or detachably connected; either mechanically or electrically, or internally communicating two elements; they may be directly connected or indirectly connected through an intermediate, and those skilled in the art will understand the specific meanings of the above terms according to specific situations.
Example 1
Combine attached figure 1 to figure 4, the utility model provides a nucleic acid constant temperature amplification subassembly, including bottom plate 1, be provided with first side post 2 and second side post 3 on the bottom plate 1, first side post 2 with it places district 4 to be equipped with the sample between the second side post 3, the outside that district 4 was placed to the sample is from last to having set gradually first heated board 5, second heated board 6 and third heated board 7 down, first heated board 5 second heated board 6 with third heated board 7 all connects first side post 2 with between the second side post 3, be equipped with first semiconductor heating refrigeration piece 8 on first heated board 5, be equipped with second semiconductor heating refrigeration piece 9 on the second heated board 6, be equipped with third semiconductor heating refrigeration piece 10 on the third heated board 7.
In this embodiment, the first side column 2 and the second side column 3 are fixed on the bottom plate 1 by bolts, which facilitates the assembly of the nucleic acid isothermal amplification module.
In this embodiment, a first partition panel 11 is disposed between the first insulation board 5 and the second insulation board 6, and a second partition panel 12 is disposed between the second insulation board 6 and the third insulation board 7. The first partition plate is used for separating the first heat-insulation plate from the second heat-insulation plate, so that the heat transfer between the first heat-insulation plate and the second heat-insulation plate is prevented from influencing the temperature of the two areas; the second partition panel is used for separating second heated board and third heated board, avoids thermal transmission to influence the temperature in two regions between second heated board and the third heated board.
In this embodiment, the first side column 2 is provided with an upper boss 21 and a lower boss 22, the second side column 3 is also provided with an upper boss 31 and a lower boss 32, the first heat insulation plate 5 is fixed on the upper boss 21 of the first side column 2 and on the upper boss 31 of the second side column 3, and the second heat insulation plate 6 is fixed on the lower boss 22 of the first side column 2 and on the lower boss 32 of the second side column 3. The upper bosses and the lower bosses on the first side column and the second side column are used for positioning the first heat-insulating plate and the second heat-insulating plate, and the nucleic acid constant-temperature amplification assembly is convenient to assemble and stable in structure.
In this embodiment, the third semiconductor heating and refrigerating sheet 10 is located at the bottom of the third heat-insulating plate 7, and the third semiconductor heating and refrigerating sheet arranged at the bottom has a better refrigerating effect on the sample placement area.
In this embodiment, the side surface of the third insulation board 7 is provided with a fluorescence detection through hole 71, so that the fluorescence result of the nucleic acid at the bottom of the sample placement area can be directly measured conveniently.
In this embodiment, a first temperature sensor 13 is disposed on the first heat-insulating plate 5 or the first semiconductor heating and cooling plate 8, and a second temperature sensor 14 is disposed on the second heat-insulating plate 6 or the second semiconductor heating and cooling plate 9. The first temperature sensor is used for acquiring the heating temperature of the first semiconductor heating and refrigerating sheet on the first heat-preserving plate, so that the temperature is prevented from being too high; similarly, the second temperature sensor is used for collecting the heating temperature of the second semiconductor heating and refrigerating piece on the second insulation board, and the temperature is prevented from being too high.
In this embodiment, the first semiconductor heating and cooling plate 8 is connected to a first temperature protection switch 15, and the second semiconductor heating and cooling plate 9 is connected to a second temperature protection switch 16. The first temperature protection switch is used as the backup protection of the first semiconductor heating and refrigerating piece, and the second temperature protection switch is used as the backup protection of the second semiconductor heating and refrigerating piece, so that when the first semiconductor heating and refrigerating piece or the second semiconductor heating and refrigerating piece is overheated, the driving loop of the first semiconductor heating and refrigerating piece or the second semiconductor heating and refrigerating piece is disconnected.
In this embodiment, the width of the end of the sample-placing region 4 away from the base plate 1 is greater than the width of the end of the sample-placing region 4 close to the base plate 1. The sample placing area with wide upper part and narrow lower part is more suitable for the existing detection reagent tube.
The utility model discloses all place the district at the sample from the sample to the nucleic acid result reaction, avoided the sample to leak the risk in nucleic acid testing process, place the district to the sample and carry out the temperature subregion from last to down, according to the difference of the required temperature of nucleic acid schizolysis reaction, cleaning process, amplification reaction, adopt the semiconductor heating refrigeration piece to carry out independent temperature regulation respectively, shortened the nucleic acid testing cycle, improved nucleic acid testing efficiency greatly.
Example 2
A nucleic acid detecting apparatus comprising the nucleic acid isothermal amplification module of embodiment 1.
The present invention and its embodiments have been described above schematically, and the description is not limited thereto, and what is shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. Therefore, if the person skilled in the art receives the teaching of the present invention, without departing from the inventive spirit of the present invention, the person skilled in the art should also design the similar structural modes and embodiments without creativity to the technical solution, and all shall fall within the protection scope of the present invention.
Claims (10)
1. The utility model provides a nucleic acid constant temperature amplification subassembly, a serial communication port, which comprises a base plate, be provided with first side post and second side post on the bottom plate, first side post with it places the district to be equipped with the sample between the second side post, the outside in district is placed to the sample is from last to having set gradually first heated board, second heated board and third heated board down, first heated board the second heated board with the third heated board is all connected first side post with between the second side post, be equipped with first semiconductor heating refrigeration piece on the first heated board, be equipped with second semiconductor heating refrigeration piece on the second heated board, be equipped with third semiconductor heating refrigeration piece on the third heated board.
2. The nucleic acid isothermal amplification assembly of claim 1, wherein a first partition plate is disposed between the first heat-insulating plate and the second heat-insulating plate, and a second partition plate is disposed between the second heat-insulating plate and the third heat-insulating plate.
3. The nucleic acid amplification assembly of claim 2, wherein the first side column and the second side column are provided with an upper boss and a lower boss, the first heat insulation plate is fixed on the upper boss of the first side column and the upper boss of the second side column, and the second heat insulation plate is fixed on the lower boss of the first side column and the lower boss of the second side column.
4. The nucleic acid constant-temperature amplification assembly according to claim 1, wherein the third semiconductor heating and refrigerating sheet is located at the bottom of the third heat-insulating plate.
5. The nucleic acid constant-temperature amplification assembly according to claim 1, wherein a fluorescence detection through hole is formed in the side surface of the third insulation board.
6. The isothermal nucleic acid amplification module of any one of claims 1 to 5, wherein a first temperature sensor is disposed on the first heat-preserving plate or the first semiconductor heating/cooling sheet.
7. The nucleic acid constant-temperature amplification assembly according to any one of claims 1 to 5, wherein a second temperature sensor is arranged on the second heat-insulation plate or the second semiconductor heating and refrigerating sheet.
8. The nucleic acid isothermal amplification module of any one of claims 1 to 5, wherein a first temperature protection switch is connected to the first semiconductor heating and cooling sheet.
9. The nucleic acid constant-temperature amplification assembly according to any one of claims 1 to 5, wherein a second temperature protection switch is connected to the second semiconductor heating/cooling sheet.
10. A nucleic acid detecting apparatus comprising the nucleic acid constant-temperature amplification module according to any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221324464.4U CN217438191U (en) | 2022-05-30 | 2022-05-30 | Nucleic acid constant temperature amplification assembly and nucleic acid detection equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221324464.4U CN217438191U (en) | 2022-05-30 | 2022-05-30 | Nucleic acid constant temperature amplification assembly and nucleic acid detection equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217438191U true CN217438191U (en) | 2022-09-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221324464.4U Active CN217438191U (en) | 2022-05-30 | 2022-05-30 | Nucleic acid constant temperature amplification assembly and nucleic acid detection equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217438191U (en) |
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2022
- 2022-05-30 CN CN202221324464.4U patent/CN217438191U/en active Active
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Address after: 310051 floor 6, building 2, No. 611, Dongguan Road, Binjiang District, Hangzhou City, Zhejiang Province Patentee after: Hangzhou Yousida Biotechnology Co.,Ltd. Address before: 310051 floor 6, building 2, No. 611, Dongguan Road, Binjiang District, Hangzhou City, Zhejiang Province Patentee before: USTAR BIOTECHNOLOGIES (HANGZHOU) Co.,Ltd. |