CN215005436U - Fluorescence detection device with a plurality of constant temperature reaction modules - Google Patents
Fluorescence detection device with a plurality of constant temperature reaction modules Download PDFInfo
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- CN215005436U CN215005436U CN202120611586.0U CN202120611586U CN215005436U CN 215005436 U CN215005436 U CN 215005436U CN 202120611586 U CN202120611586 U CN 202120611586U CN 215005436 U CN215005436 U CN 215005436U
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Abstract
The utility model provides a fluorescence detection device with a plurality of constant temperature reaction modules, including constant temperature reaction module and motion scanning subassembly, the control unit is connected with control between constant temperature reaction module and the motion scanning subassembly, the motion scanning subassembly includes linear motion drive part and arouses and the collection module with the signal that linear motion drive part drive is connected, the signal arouses and has arouses the scanning head on the collection module, arouse the scanning head and can realize fluorescence signal detection to the reaction tube in the warm bath that constant temperature reaction module has; the isothermal reaction modules are provided in plurality, are structurally independent of each other, and can be controlled independently from each other. The utility model discloses have a plurality of constant temperature reaction module and can be by independent control respectively, can carry out the detection of a plurality of different procedures simultaneously, greatly improve detection efficiency, can satisfy the application demand of hospital, the multiple environment of inspection institution.
Description
Technical Field
The utility model belongs to the technical field of supplementary external diagnosis utensil, concretely relates to fluorescence detection device with a plurality of constant temperature reaction module.
Background
The in vitro amplification technology of nucleic acid is one of the most commonly used research methods and tools in the field of life science, has been widely applied in the fields of molecular biology, medical diagnosis, pathogenic microorganism detection and the like since Polymerase Chain Reaction (PCR) in the 80 th 20 th century, and particularly has been the development of the fluorescence PCR technology, wherein a fluorescence detection specific probe is introduced in the PCR process by the fluorescence PCR technology, and the detection of the existence of a target in a sample to be detected is realized through the change of a fluorescence signal in the reaction amplification process. The fluorescence PCR technology requires the instrument to carry out continuous heating and cooling amplification, and has higher requirements on detection equipment.
Isothermal nucleic acid amplification method, also known as isothermal nucleic acid amplification method, is a nucleic acid amplification method that has been rapidly developed since the 21 st century. Unlike PCR, which needs variable temperature denaturation to release double strand amplification, isothermal nucleic acid amplification method is to add different nucleic acid polymerases and specific primers at a constant temperature to achieve rapid amplification of nucleic acid. The isothermal amplification technology can basically realize the detection of target nucleic acid within 1 hour, and some can even realize the detection within 20 min. In the prior art, a method and a kit for detecting one or more target nucleic acid sequences to be detected in a single tube by introducing a probe (RNHP) containing an RNA base and high-temperature resistant RNaseH2 into an isothermal amplification technology realize real-time isothermal detection of single-target or multi-target nucleic acids (for example, described in patent document CN 109750091A). Isothermal detection equipment in the current market mostly adopts two-channel and single-channel detection, and the detection requirements of a plurality of pathogens cannot be met.
SUMMERY OF THE UTILITY MODEL
Therefore, the to-be-solved technical problem of the utility model is to provide a fluorescence detection device with a plurality of constant temperature reaction modules, have a plurality of constant temperature reaction modules and can be by independent control respectively, can carry out the detection of a plurality of different procedures simultaneously, greatly improve detection efficiency, can satisfy the application demand of hospital, the multiple environment of inspection institution.
In order to solve the above problems, the present invention provides a fluorescence detection device with a plurality of constant temperature reaction modules, which is characterized in that the fluorescence detection device comprises a constant temperature reaction module, a motion scanning assembly, a display assembly and a control unit, wherein the control unit is in control connection with the constant temperature reaction module and the motion scanning assembly, the motion scanning assembly comprises a linear motion driving assembly and a signal excitation and collection module in drive connection with the linear motion driving assembly, the signal excitation and collection module is provided with an excitation scanning head, and the excitation scanning head can realize fluorescence signal detection on a reaction tube in a temperature bath provided by the constant temperature reaction module; the isothermal reaction modules are mutually independent in structure and can be respectively and independently controlled; the display component is used for detecting program selection or information input, controlling the operation of the constant temperature reaction module and the motion scanning assembly and controlling the output of a detection result.
Optionally, a hot cover is arranged at the notch of the warm bath groove, and a cover body heating peltier is arranged in the hot cover.
Optionally, the heat cover includes a plastic casing, the plastic casing includes a sealing plate body fastened to the notch of the warm bath and a cover body heating peltier clamp located between the sealing plate body and the outer side plate body.
Optionally, a plurality of elastic pieces are further arranged between the sealing plate body and the outer side plate body, and one end of the hot cover is connected with the notch of the warm bath groove through a reset torsion spring; and/or a tank body heating Peltier is arranged in the warm bath, and the control unit adopts a pulse width modulation mode to carry out heating control on the cover body heating Peltier and the tank body heating Peltier.
Optionally, a thermistor sensor is further disposed at the thermal cover and the warm bath, and is in communication connection with a control unit of the fluorescence detection device.
Optionally, the fluorescence detection apparatus further includes a display unit, a printing unit, and a code scanning unit, the display unit, the printing unit, and the code scanning unit are all in communication connection with the control unit, the display unit is used for detecting program selection or information input, controlling the operation of the constant temperature reaction module and the motion scanning assembly, and controlling the output of the detection result, the printing unit is used for outputting the detection result, and the code scanning unit is used for inputting sample information and/or item information.
Optionally, the fluorescence detection apparatus further includes a wireless network module, which is used for network connection with a laboratory information management system and/or a hospital information system.
The utility model provides a pair of fluorescence detection device with a plurality of constant temperature reaction modules, fluorescence detection device has a plurality of constant temperature reaction modules and a plurality of constant temperature reaction modules can be by independent control respectively, thereby makes fluorescence detection device can carry out the detection of a plurality of different procedures simultaneously also can be simultaneously or asynchronous (make fluorescence detection device can be applicable to the fluorescence detection demand of the sample of different times) carry out multiple detection project, greatly improves detection efficiency, can satisfy the application demand of hospital, the multiple environment of inspection institution.
Drawings
FIG. 1 is a schematic structural diagram of a fluorescence detection apparatus having a plurality of isothermal reaction modules according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of the isothermal reaction module in FIG. 1;
FIG. 3 is a schematic view of the construction of the heat cover of FIG. 2;
FIG. 4 is a schematic diagram of the motion scanning assembly of FIG. 1;
FIG. 5 is a schematic view of the amplification map and result output interface displayed by the display unit after the reaction of the fluorescence detection apparatus with a plurality of isothermal reaction modules of the present invention is completed.
The reference numerals are represented as:
1. a constant temperature reaction module; 11. a warm bath tank; 12. a hot lid; 121. heating the Peltier by the cover body; 122. a plastic housing; 123. a sealing plate body; 124. an outer plate body; 125. an elastic member; 126. a return torsion spring; 127. a thermistor sensor; 2. a motion scanning assembly; 21. a linear motion drive member; 22. a signal excitation and acquisition module; 3. a display section; 4. a printing component; 5. a code scanning component; 100. a housing.
Detailed Description
Referring to fig. 1 to 5 in combination, according to an embodiment of the present invention, a fluorescence detection apparatus with a plurality of isothermal reaction modules is provided, including a housing 100, an isothermal reaction module 1 disposed on the housing 100, a motion scanning assembly 2 and a control unit disposed in the housing 100, specifically, the housing 100 includes an upper housing and a bottom housing detachably connected to each other, a containing cavity is formed between the upper housing and the bottom housing, the control unit specifically includes a control circuit board and electronic components such as a processor disposed thereon, the control circuit board is disposed on the bottom housing, the control unit is connected to the isothermal reaction module 1 and the motion scanning assembly 2 for controlling the isothermal reaction module 1 and the motion scanning assembly 2, specifically, for example, controlling the temperature rise of the isothermal reaction module 1 and the motion state and signal excitation and acquisition of the motion scanning assembly 2 The device comprises a motion scanning assembly 2, a linear motion driving assembly 21, a signal excitation and acquisition module 22 connected with the linear motion driving assembly 21 in a driving manner, an excitation scanning head arranged on the signal excitation and acquisition module 22, wherein the excitation scanning head can detect fluorescence signals of reaction tubes in a temperature bath 11 of the constant temperature reaction module 1, the constant temperature reaction modules 1 are provided with a plurality of modules, the constant temperature reaction modules 1 are mutually independent in structure, and the constant temperature reaction modules 1 can be respectively and independently controlled (for example, are heated by independent control to form constant temperature). In the technical scheme, the fluorescence detection device is provided with a plurality of constant temperature reaction modules 1 and a plurality of constant temperature reaction modules 1 which can be respectively and independently controlled, so that the fluorescence detection device can simultaneously detect a plurality of different programs, namely can simultaneously or asynchronously (so that the fluorescence detection device can be suitable for the fluorescence detection requirements of samples at different times) carry out various detection items, the detection efficiency is greatly improved, and the application requirements of hospitals and inspection institutions in various environments can be met. The housing 100 is designed according to ergonomics, so that the size is small and the carrying is convenient.
In one embodiment, the signal excitation and collection module 22 has four fluorescence detection signal paths, and the central wavelength of the excitation light/the central wavelength of the emission light of each path are 480nm/518nm, 532nm/564nm, 571nm/610nm, and 640nm/692nm, respectively, and simultaneously satisfies the above four signal excitation and reception, so as to realize the detection of multiple targets.
Optionally, a thermal cover 12 is arranged at a notch of the warm bath 11, the thermal cover 12 is used for sealing and covering a gate of the reaction tube in a fluorescence detection process to evaporate the reaction liquid, a cover body heating peltier 121 is arranged in the thermal cover 12, and the thermal cover 12 can be heated to effectively prevent evaporated water vapor from condensing on the thermal cover 12, so that the stability of the reaction liquid amount is ensured. Specifically, the hot lid 12 includes a plastic casing 122, and can realize effective isolation between the temperature in the warm bath 11 and the temperature of the external environment, and further satisfy the requirement of constant temperature, the plastic casing 122 includes a sealing plate body 123 fastened to the notch of the warm bath 11 and a cover body heating peltier 121 located between the sealing plate body 123 and the outer side plate body 124.
In some embodiments, a plurality of elastic members 125 are further disposed between the sealing plate 123 and the outer plate 124, the elastic members 125 are disposed to ensure the sealing performance between the sealing plate 123 and the notch of the warm bath 11, and one end of the thermal cover 12 is connected to the notch of the warm bath 11 through a return torsion spring 126, and it can be understood that a locking member, such as a buckle, is disposed between the other end of the thermal cover 12 and the notch of the warm bath 11, so that the thermal cover 12 can seal the notch of the warm bath 11 during the fluorescence detection process, and the thermal cover 12 can be easily opened when taking and placing the reaction tube.
The inside of the warm bath 11 is provided with a tank body heating peltier, the control unit performs heating control on the cover body heating peltier 121 and the tank body heating peltier in a Pulse Width Modulation (PWM) mode, the heating peltier is heated in the PWM mode, and the temperature control is stable, accurate and energy-saving compared with a resistance current limiting method.
Further, a thermistor sensor 127 is further disposed at the thermal cover 12 and the warm bath 11, and is in communication connection with a control unit of the fluorescence detection device, the thermistor sensor 127 is used for detecting corresponding temperatures in the thermal cover 12 and the warm bath 11, and feeding back the detected real-time temperature to the control unit, and the control unit controls the operation of the cover body heating peltier 121 and the tank body heating peltier according to a preset target control temperature, so that the real-time temperature is controlled to be near the target control temperature, and it can be understood that a radiator is disposed corresponding to the heating peltier to radiate heat of a cold end of the heating peltier, and the heating efficiency is improved.
Further, the fluorescence detection device further comprises a display part 3, a printing part 4 and a code scanning part 5, wherein the display part 3, the printing part 4 and the code scanning part 5 are all in communication connection with the control unit, that is, the display part 3, the printing part 4 and the code scanning part 5 are all controlled by the control unit, so as to coordinate to realize the fluorescence detection process; the display component 3 is used for detecting program selection or information input, controlling the operation of the constant temperature reaction module 1 and the motion scanning component 2 and controlling the output of a detection result, the printing component 4 is used for outputting a detection result, specifically, the printing component is used for printing the detection result in real time, and the information of the printing result comprises information such as a sample number, a reagent batch number, a detection item, a Tt value, a result judgment, concentration and the like; the code scanning component 5 is used for inputting sample information and/or item information, realizing identification of detection items and sample numbers and transmission of information, and simplifying operation, and the code scanning component 5 is preferably configured to be capable of identifying bar codes and two-dimensional codes, simplifying manual input operation, and shortening operation time. Further, the detection item information covered by the two-dimensional code includes all information of reaction process operation and result analysis, including batch number, analysis method, positive judgment value and the like, but is not limited to the above information.
Optionally, the fluorescence detection apparatus further comprises a wireless network module, which is used for being connected with a laboratory information management system and/or a hospital information system through a network, so that the fluorescence detection apparatus can be moved and arranged as required, and real-time transmission of detection results is realized.
The control unit is further configured to: analyzing and judging the quality control according to the detection item information to judge whether the current reaction is effective or not; judging the sample according to the detection value of the sample (the reaction reagent in the reaction tube), and outputting a judgment result; the inaccurate diagnosis caused by the wrong detection result caused by the failure or pollution of the reaction reagent is avoided through quality control judgment; calculating and outputting results according to the calibration curve information of the project, quantitatively analyzing the results, judging current reaction detection according to the quality control information of the detection project, and calibrating the project calibration curve in real time according to the judgment result to improve the accuracy of quantitative detection; specifically, calibration range parameters, namely different confidence intervals of the calibration curve, are introduced during analysis and analysis of quantitative results in output, the effectiveness of the calibration curve is judged through the calibration range parameters, when the calibration range parameters exceed the calibration range, the secondary reaction is judged to be ineffective, the detection concentration of an output sample is judged to be ineffective, and the calibration curve is calibrated through the results of the quality control reaction holes.
The technical solution of the present invention is further described below with reference to a specific embodiment.
The reaction tube after liquid adding is placed in a warm bath 11, after a hot cover 12 is covered, sample information and project information are input through a code scanning component 5 (such as a micro code scanner), or program selection or information input and operation program selection are carried out on a display component 3 (a display screen of a man-machine interaction system), the temperature of the device is automatically raised after reaction starting is controlled, a signal excitation and acquisition module 22 (which can also be understood as a fluorescent signal acquisition system) of a motion scanning component 2 (which can also be understood as a fluorescent detection module) reciprocates according to specified time, fluorescent signals are simultaneously acquired in real time, after the reaction is finished, the effectiveness of a reaction reagent is judged through a quality control hole according to fluorescent signal values collected at different times, after the reaction reagent is determined to be effective, the sample result is analyzed through the change of the fluorescent signal values at different times of a sample hole, and the sample result is judged through the project positive judgment value, the detection result is output through the display part 3 and the printing part 4 (such as an auxiliary printer), or the result is transmitted to the inside of the hospital system through a wireless network, so that the detection and the output are realized.
The control unit comprises a main board, a processor and a data analysis system to control the detection process of the equipment, the result judgment algorithm realizes result judgment and output through different algorithms, and corresponding screen display is output on the display part as shown in fig. 5.
Tests prove that the device has better analysis and detection sensitivity. The detection results of the same reagents are compared with the fluorescent PCR instrument with wide fluorescence on the market in parallel, the results are shown in tables 1 and 2, and the amplification map and the result output are shown in FIG. 5. As can be seen from tables 1 and 2, both CV values are below 5% compared to ABI7500 with similar repeatability; when a 103Copies/mL sample is detected, the detection rate of the two samples is the same, when a 5X 102Copies/mL sample is detected, the detection rate of the real-time constant-temperature fluorescence detection system is 95 percent and is superior to 85 percent of ABI7500, and the lowest detection concentration of the real-time constant-temperature fluorescence detection is lower than ABI 7500. FIG. 5 shows the amplification map and the result output function, and the automatic normalization process of the system makes the amplification curve clear at a glance; automatically judging whether the result is negative or positive by setting a threshold value; the button on the right side of the screen can select results to print, the results can be directly printed out through a mini printer integrated on the right part of the equipment, the results can also be selected firstly to be exported, and test data can be exported to a U disk.
TABLE 1 summary of the comparison results of different model testing
TABLE 2 comparison of the test results of different models
It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.
The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (6)
1. The fluorescence detection device with the constant temperature reaction modules is characterized by comprising a constant temperature reaction module (1), a motion scanning assembly (2), a display assembly (3) and a control unit, wherein the control unit is in control connection with the constant temperature reaction module (1) and the motion scanning assembly (2), the motion scanning assembly (2) comprises a linear motion driving assembly (21) and a signal excitation and acquisition module (22) in drive connection with the linear motion driving assembly (21), an excitation scanning head is arranged on the signal excitation and acquisition module (22), and the excitation scanning head can realize fluorescence signal detection on a reaction tube in a temperature bath (11) of the constant temperature reaction module (1); the isothermal reaction modules (1) are provided with a plurality of modules, the isothermal reaction modules (1) are mutually independent in structure, and the isothermal reaction modules (1) can be respectively and independently controlled; the display component (3) is used for detecting program selection or information input, controlling the operation of the constant temperature reaction module (1) and the motion scanning assembly (2) and outputting and controlling a detection result.
2. The fluorescence detection device according to claim 1, wherein a thermal cover (12) is disposed at the notch of the warm bath (11), and a cover body heating Peltier (121) is disposed in the thermal cover (12).
3. The fluorescence detection device according to claim 2, characterized in that said thermal cover (12) comprises a plastic casing (122), said plastic casing (122) comprising a sealing plate (123) engaged with a notch of said warm bath (11) and a cover heating peltier (121) interposed between said sealing plate (123) and an outer plate (124) facing the user.
4. The fluorescence detection device according to claim 3, wherein a plurality of elastic members (125) are further arranged between the sealing plate body (123) and the outer side plate body (124), and one end of the hot cover (12) is connected with the notch of the warm bath (11) through a reset torsion spring (126); and/or a tank body heating Peltier is arranged in the warm bath (11), and the control unit is used for heating and controlling the cover body heating Peltier (121) and the tank body heating Peltier in a pulse width modulation mode.
5. The fluorescence detection device according to claim 4, wherein a thermistor sensor (127) is further provided at the thermal cover (12) and the warm bath (11), and is connected to a control unit of the fluorescence detection device in communication.
6. The fluorescence detection device of claim 1, further comprising a wireless network module for network connection with a laboratory information management system and/or a hospital information system.
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| CN202120611586.0U CN215005436U (en) | 2021-03-26 | 2021-03-26 | Fluorescence detection device with a plurality of constant temperature reaction modules |
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| CN202120611586.0U CN215005436U (en) | 2021-03-26 | 2021-03-26 | Fluorescence detection device with a plurality of constant temperature reaction modules |
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Inventor after: Liu Licheng Inventor after: Yang Honglei Inventor after: Feng Huahua Inventor after: Wang Pengzhi Inventor after: Hu Xiaoxu Inventor after: He Yanchen Inventor after: Sun Diwen Inventor after: Zhao Xiangsheng Inventor before: Liu Licheng Inventor before: Yang Honglei Inventor before: Feng Huahua Inventor before: Wang Pengzhi Inventor before: Hu Xiaoxu Inventor before: He Yanchen Inventor before: Sun Diwen Inventor before: Zhao Xiangsheng |