TW201843446A - Photothermal reaction analyzer - Google Patents

Abstract

The present disclosure provides a photothermal reaction analyzer. The photothermal reaction analyzer comprises a plurality of photothermal reaction units. Each of the photothermal reaction units includes a container assembly having a slot, a sample container is fitted in the slot and for containing reagents for performing a photothermal reaction, a temperature control module coupled to the container assembly for controlling a first temperature program for the container assembly and a second temperature program for the sample container, and an optics module coupled to the container assembly and for emitting and detecting light. In each of the photothermal reaction units, the optics module is stationary relative to the container assembly.

Description

Photothermal reaction analyzer

The disclosure relates to an in vitro diagnostic instrument. Further, a photothermal reaction analyzer and an immunofluorescence reaction, an isothermal polymerase chain reaction (isothermal PCR), and a reverse transcriptase polymerase chain reaction are performed by the photothermal reaction analyzer. Reverse-transcriptase PCR, multiple sets of polymerase chain reaction (multiplex PCR), digital polymerase chain reaction (digital PCR), traditional polymerase chain reaction or real-time polymerase chain reaction (real-time PCR) The method is related.

A polymerase chain reaction (PCR) amplifies one or more pieces of DNA from a single or multiple target templates. The polymerase chain reaction is typically performed in a closed, temperature controlled space. The user can use a special temperature setting to perform the polymerase chain reaction. Currently, polymerase chain reaction analyzers on the market are mostly thermal cyclers capable of controlling the temperature of the polymerase chain reaction. The C-1000 ^TM Touch Thermal Cycler is a traditional polymerase chain reaction analyzer that enables the user to perform two sets of temperature settings simultaneously. Currently, polymerase chain reaction reactors on the market can provide a plurality of accommodation spaces, slots or holes for placing sample containers. The SimpliAmp ^TM Thermal Cycler is a traditional polymerase chain reaction analyzer that provides multiple slots for sample placement and a remote control function. However, conventional polymerase chain reaction analyzers are unable to perform other reactions commonly used in in vitro diagnostics, such as direct or indirect immunofluorescence reactions.

One of the polymerase chain reaction reactions is real-time PCR or reverse-transcriptase PCR. Since fluorescent molecules are often used in the reaction, the instant polymerase chain reaction can optically observe the internal space at which the reaction proceeds to obtain experimental results. The fluorescent molecules can be excited by a laser of a specific wavelength and emit a fluorescent signal that can be used as a reaction indicator. Most instant polymerase chain reaction analyzers are equipped with optical components to detect fluorescence during the reaction. The fluorescent molecules in the sample container can be excited by the excitation light from the excitation light source, and the detecting element detects the fluorescent signal from the excited fluorescent molecules. Therefore, the optical component needs to be aligned with the sample container to receive the fluorescent signal. When multiple reactions occur in multiple sample containers of the instant polymerase chain reaction analyzer, the optical element needs to be along the XY plane in order to align multiple sample containers housed in a plurality of slots. Pan and move from one slot to another. CFX96 ^TM Real-Time PCR Detection System shuttle having an optical element, may be moved in a fluorescent signal detected in each well, and the plurality of fluorescent signal mainly from the green LED above each well of the 96 well plate. In summary, the movement of optical components in conventional instant polymerase chain reaction analyzers requires precise alignment between the optical component and several sample containers, slots or wells, which provides a reliable real-time polymerase chain lock. The key to the results of the reaction experiments.

Because of the need for precise alignment between optical components and several sample containers, conventional instant polymerase chain reaction analyzers are often stationary and are marketed as non-portable instruments. Various movements or tilts of conventional instant polymerase chain reaction analyzers can cause optical components to be misaligned. The optical component will need to be calibrated after moving the immobilized real-time polymerase chain reaction analyzer, which limits the application of the conventional instant polymerase chain reaction analyzer. Therefore, it is not feasible to perform an immediate polymerase chain reaction outside the laboratory.

Further, a conventional polymerase chain reaction reaction analyzer or an instant polymerase chain reaction reaction analyzer uses a single heating block as a thermal conductor to change the temperature of the sample container. The heating block is in direct contact with the sample containers to raise the temperature of the sample containers. The heating block may also be in direct contact with the surrounding environment or be housed in a thin housing. However, the sample containers in a single heating block may also be affected by the temperature of the surrounding environment; if the surrounding environment is extremely cold or extremely hot, the sample containers in the polymerase chain reaction analyzer are The temperature may be affected. Reactions performed at incorrect temperatures may be inefficient and unreliable, so polymerase chain reaction analyzers that traditionally use a single heating block design are not suitable for use outdoors or in extreme environments. .

Therefore, there is a need for a photothermal reaction analyzer having multiple thermal conductor designs such that the temperature of the surrounding environment has little or no effect on the temperature of the sample container.

Therefore, there is a need for a photothermal reaction analyzer that is portable, lightweight, and has fixed optical components such that the optical components do not need to be recalibrated after being moved.

There is also a need for a photothermal reaction analyzer capable of performing temperature cycle related applications and immunofluorescence reactions.

There is also a need for a photothermal reaction analyzer having laser, infrared light, optical detection, and temperature sensing elements to perform polymerase chain reaction or other excitation-related reactions.

There is also a need for a photothermal reaction analyzer that detects polymerase chain reaction by taking an image of the solvent in the sample container.

The present disclosure relates to a portable photothermal reaction analyzer having a fixed optical element and a plurality of thermal conductors.

According to one embodiment below, the present disclosure provides a photothermal reaction analyzer. The photothermal reaction analyzer includes a plurality of photothermal reaction units. Each of the photothermal reaction units includes: a container assembly including a tank; and a container, the sample container can be housed in the tank and can accommodate reagents required to perform a photothermal reaction; a temperature control module, The temperature control module is coupled to the container assembly, and the temperature control module controls a first temperature program corresponding to the container assembly and a second temperature program corresponding to the sample container; and an optical module, the optical module The set is stationary relative to the container assembly.

According to one embodiment, the container assembly further includes at least one opening that communicates with the slot, and an optical module is coupled to the container assembly through the opening.

According to one embodiment, the container assembly further includes at least two openings, the at least two openings are in communication with the slot, and each optical module includes at least one excitation light source to emit an excitation light and at least one detector to detect A visible light or a fluorescent signal. The excitation light source and the detector are respectively coupled to the container assembly through the two openings.

According to one embodiment below, the optical module is configured to emit a blue excitation light, a green excitation light, an orange excitation light, a red excitation light, or a combination thereof.

According to one embodiment below, the optical module is configured to detect a green fluorescent light, a cyan fluorescence, an orange fluorescent light, a red fluorescent light, or a combination thereof.

According to one embodiment below, the optical module is further configured to be capable of filtering the fluorescence to a range of wavelengths.

According to one embodiment, the temperature control module includes: a heating element, the heating element is coupled to one of the container assemblies through the opening to heat the sample container; a container assembly temperature sensor; the same temperature sensor And a cooling element. The heating element can be an electromagnetic radiation generator.

According to one embodiment below, the excitation light source comprises a blue excitation light source, a green light excitation light source, a orange light excitation light source or a red light excitation light source.

According to one embodiment, the detector includes a green fluorescent detector, a blue fluorescent detector, an orange fluorescent detector or a red fluorescent detector.

According to one embodiment below, the detector comprises a photodiode (PD), an avalanche photodiode (APD), a photomultiplier tube (PMT), and a photocell. A silicon photomultiplier (SIPM) or a combination of the above.

According to one embodiment, the optical module further includes a filter disposed between the excitation light source and the detector to filter the fluorescence to a wavelength range.

According to one embodiment below, the detector includes one or more image elements to capture at least one image of the inside of the container assembly.

Another embodiment of the present disclosure provides a photothermal reaction analyzer. The photothermal reaction analyzer includes a plurality of container assemblies, a container assembly receiving device, a plurality of temperature control modules, and a plurality of optical modules. The container assembly receiving device includes a plurality of recesses, and each recess can accommodate a container assembly. The sample container can be housed in a tank of a container assembly and the sample container can hold the reagents required to perform a photothermal reaction. The temperature control module is coupled to the container assembly, and the temperature control module controls a third temperature program corresponding to the container assembly receiving device and a second temperature program corresponding to the sample container. Each optical module is coupled to a container assembly and can emit and receive light. The optical module is stationary relative to the container assembly.

Another embodiment of the present disclosure provides a photothermal reaction analyzer. The photothermal reaction analyzer includes a container assembly block. The container assembly block includes: a plurality of slots; a plurality of sample containers; a temperature control module; and a plurality of optical modules. Each sample container can be housed in a tank and can contain the reagents needed to perform a photothermal reaction. The temperature control module is coupled to the container assembly block, and the temperature control module controls a fourth temperature program corresponding to the container assembly block and a second temperature program corresponding to the sample container. Each optical module is coupled to a slot in the body of the container assembly. The optical module is stationary relative to the container assembly.

According to one embodiment, the container assembly block further includes a plurality of openings, and at least two openings are in communication with the slots, and each optical module includes at least one excitation light source to emit an excitation light and at least one detector to Detect a visible light or a fluorescent signal. The excitation light source and the detector are respectively coupled to the container assembly block through the two openings.

According to one embodiment, the temperature control module includes: a plurality of heating elements to heat the sample containers; a container assembly block temperature sensor; the same temperature sensor; and a cooling element. Each of the heating elements is coupled to a slot in the block of the container assembly through the openings. The heating element is an electromagnetic radiation generator.

According to one embodiment below, the photothermal reaction performed by the photothermal reaction analyzer or the photothermal reaction analysis unit is an immunofluorescence reaction, an isothermal polymerase chain reaction (isothermal PCR), and a reverse transcription polymerase chain. Reverse-transcriptase PCR, multiple sets of polymerase chain reaction (multiplex PCR), one-digit polymerase chain reaction (digital PCR), a traditional polymerase chain reaction or an instant polymerase chain reaction (real-time PCR).

According to one embodiment below, the photothermal reaction analyzer further includes a control module to control the temperature control module and the optical module.

In order to make the schema clear and concise, the symbols representing the corresponding components in different drawings may be repeated. Further, in order to make the embodiments fully understandable, the present specification also describes various details in the various embodiments. However, those skilled in the art can also implement the following embodiments without the many details described above. The illustrations of the present disclosure are not intended to represent the dimensions and proportions of the components, and may be exaggerated to better illustrate the details and features of the components. The purpose of the present specification is not to limit the contents of the following embodiments.

Please refer to FIG. 1. The present disclosure provides a photothermal reaction unit 10. The photothermal reaction analyzer includes a plurality of photothermal reaction units 10. Each photothermal reaction unit 10 includes a container assembly 140 having a tank 141, a similar container 110, and the sample container 110 can be received within the tank 141 and can contain reagents required to perform a photothermal reaction. A temperature control module 120 is coupled to the container assembly 140 and controls a first temperature program corresponding to the container assembly 140 and a second temperature program corresponding to the sample container 110. An optical module 130 is coupled to the container. Component 140 can also emit or receive light. In each of the photothermal reaction units 10, the optical module 130 is stationary relative to the container assembly 140.

The sample container 110 is a transparent container and has a closed space for receiving the reagents. The sample container 110 can be a microcentrifuge tube, a capillary tube, a microfluidic chip, a pipette tip or other container having a transparent tube wall and an enclosed space. The reagent can include one or more thermogenic reactants. The heat generating reactant can be irradiated with electromagnetic radiation to generate heat. The reactant may be a transition metal material, and the transition metal material may be a transition metal oxide, a transition metal hydroxide, or a nitride, phosphide or arsenide doped transition metal of a Group III metal or A transition metal oxide, or a cerium oxide doped transition metal, transition metal oxide or transition metal hydroxide. The temperature of the reactants rises when it is irradiated with infrared rays.

Further, the reagent may also include an agent required for an immunofluorescence reaction, a polymerase chain reaction (PCR) or an instant polymerase chain reaction (real-time PCR). The reagents required for the immunofluorescence reaction may include a test substance, a fluorescent dye, a labeling reagent, or any other reagent required to perform an immunofluorescence reaction. The tests required for the polymerase chain reaction may include a test substance, a polymerase, a deoxynucleoside triphosphate (dNTPs), a primer, a probe, a fluorescent dye, and a template. A template sequence or any other reagent required to perform a polymerase chain reaction or an instant polymerase chain reaction.

The temperature control module 120 includes a heating element 121 coupled to the container assembly 140 to heat the sample container 110 through an opening (not shown). The temperature control module 120 further includes a container assembly. The temperature sensor 124, the same temperature sensor 122, and a cooling element 123. The temperature control module 120 controls a first temperature program corresponding to the container assembly 140 and a second temperature program corresponding to the sample container 110. The first temperature program adjusts the temperature of the container assembly 140. Preferably, the first temperature program is designed such that the container assembly 140 is at a constant temperature that is unaffected by ambient temperature. The temperature of the container assembly 140 can be raised or lowered according to the first temperature program. Therefore, the photothermal reaction in the sample container 110 can be performed under a stable condition, and the temperature of the surrounding environment has little or no influence on the influence thereof. The second temperature program is a pre-programmed program and enables the sample container 110 to be in one or more predetermined temperatures. The second temperature program can include one or more predetermined temperatures, one or more time intervals for the sample container 110 to spend a certain length of time at a predetermined temperature, and one or more predetermined times The interval changes the temperature from one temperature to another. For example, the second temperature program can cause the sample container 110 to be at 65 ° C for 10 minutes, at 95 ° C for 5 minutes, and within 60 seconds from 65 ° C to 95 ° C. The second temperature program can be designed by the user to achieve an immunofluorescence reaction, an isothermal polymerase chain reaction (isothermal PCR), a reverse transcription polymerase chain reaction (reverse-transcriptase PCR), a multi-set polymerization. The temperature required for multiplex PCR, one-digit polymerase chain reaction (digital PCR), a conventional polymerase chain reaction, or an instant polymerase chain reaction (real-time PCR). The photothermal reaction analyzer of the present disclosure is capable of performing an immunofluorescence reaction, an isothermal polymerase chain reaction, a reverse transcription polymerase chain reaction, a multi-set polymerase chain reaction, and a digital polymerase chain lock. Reaction, a conventional polymerase chain reaction, an immediate polymerase chain reaction or other temperature-limited in vitro reaction. This traditional polymerase chain reaction may require a specific temperature to perform denaturation, hydration, and elongation. However, isothermal polymerase chain reaction or immunofluorescence may require only one or a few specific temperatures.

The temperature control module 120 includes a heating element 121. The heating element 121 is controlled by the second temperature program to raise the temperature of the sample container 110. Preferably, the heating element 121 is placed at the bottom of the sample container 110. The heating element 121 can be a single electromagnetic radiation generator that emits one or more electromagnetic radiation. The electromagnetic radiation emitted by the heating element 121 has a frequency in the range of 200 kHz to 500 THz. Preferably, the heating element 121 is an infrared laser diode. The heating element can emit electromagnetic radiation to cause the heat generating reactant to raise the temperature of the reagents within the sample container 110. The electromagnetic radiation having a particular wavelength can induce an increase in the temperature of the reactants in the reagent.

In another embodiment, the temperature control module 120 can further include the same temperature sensor 122. The sample temperature sensor 122 is in direct contact with the sample container 110 to sense the temperature of the sample container 110. Preferably, the sample temperature sensor 122 is a thermocouple.

In another embodiment, the sample temperature sensor 122 can also be integrated with the heating element 121 on the same component to reduce the power consumption and volume of the sample temperature sensor 122 and the heating element 121.

In another embodiment, the temperature control module 120 further includes a cooling element 123 to cool the container assembly 140. The cooling element 123 is not in direct contact with the sample container 110. The cooling element 123 is controlled by the first temperature program. Preferably, the cooling element 123 can be an air cooling element, a liquid cooling element, a liquid cold-air cooled hybrid cooling element, and a semiconductor cooling element. The air cooling element can be a fan or an element capable of generating an air flow to cool the ambient temperature. The semiconductor cooling element can be a semiconductor cooling plate or wafer, such as a thermo cooler (TE cooler). The sample container 110 is heated by the heating element 121 and cooled by the container assembly 140, wherein the container assembly 140 is cooled by the cooling element 123. The temperature of the sample container 110 is detected by the same temperature sensor 122. The heating element 121, the sample temperature sensor 122, and the cooling element 123 are communicatively coupled to one another and are capable of detecting the temperature of the reagent in the sample container 110.

The optical module 130 can be configured to emit a blue excitation light, a green light excitation light, an orange light excitation light, a red light excitation light, or a combination thereof. The optical module 130 can also be configured to detect a green fluorescent light, a cyan fluorescence, an orange fluorescent light, a red fluorescent light, or a combination thereof. The optical module 130 can also be configured to filter the fluorescence to a range of wavelengths.

The optical module 130 includes one or more excitation sources and one or more detectors. The optical module 130 can also be configured to have different excitation sources to emit excitation light of different wavelengths, or have different detectors to detect different wavelengths of fluorescence. The excitation light sources 131a and 131b can also emit an excitation light to the reagent in the sample container 110. The fluorescent dye in the reagent emits fluorescence when excited by the excitation light emitted from the excitation light sources 131a and 131b. The excitation light sources 131a and 131b can also be one or more light sources. The excitation light sources 131a and 131b can selectively emit excitation light of different wavelengths, thereby causing different fluorescent dyes to emit fluorescence. The detectors 132a and 132b can selectively detect fluorescent light emitted by different fluorescent dyes.

In the present disclosure, the optical module 130 includes at least two excitation light sources 131a and 131b. The excitation light source 131a can be a blue excitation source and the excitation source 131b can be a green excitation source. Other excitation sources may also be used in the optical module 130, such as a blue fluorescent excitation source, an orange fluorescent excitation source, or a red fluorescent excitation source. In the present embodiment, the excitation light source 131a emits electromagnetic radiation having a wavelength range of about 450 to 495 nm, and the excitation light source 131b emits electromagnetic radiation having a wavelength range of about 495 to 570 nm. Preferably, the excitation light source 131a or 131b is a light emitting diode (LED) or a semiconductor laser diode (LD), such as a blue light emitting diode, a blue semiconductor laser diode, and a green light. A light emitting diode or a green semiconductor laser diode. From the type of the fluorescent dye in the reagent, it is possible to determine which excitation light source to use. For example, if the fluorescent dye is SYBR, the excitation light source 131a can be automatically or manually activated; if the fluorescent dye is ROX, the excitation light source 131b can also be automatically or manually activated. According to the experimental requirements, the user can choose a wider variety of other excitation sources.

The fluorescent detectors 132a and 132b can detect light of one or more wavelengths. A detector 132a can be configured to detect different wavelengths of fluorescence that are excited by the plurality of excitation sources 131a and 131b. In addition, different wavelengths of fluorescence excited by the plurality of excitation light sources 131a and 131b can also be detected by the plurality of detectors 132a and 132b.

In another embodiment, the detector 132a is a green fluorescent detector and the detector 132b is a red fluorescent detector. Other fluorescent detectors can also be used in the detectors described in the present disclosure, such as a blue fluorescent detector or an orange fluorescent detector. The detector is based on the fluorescent dye type in the reagent, the detector 132a detects green fluorescent light, and the detector 132b detects red in a wavelength range of 620 nm to 750 nm. Fluorescent. Preferably, the detector 132a is a photodiode (PD), an avalanche photodiode (APD), a photomultiplier tube (PMT), and a photomultiplier tube. (silicon photomultiplier; SIPM) or other photomultiplier or diode components. The detectors 132a and 132b can be selected or activated by the type of fluorescent light that is excited in the reagent. For example, if the fluorescent dye is SYBR, it can be excited by the excitation light emitted by the excitation light source 131A, and the detector 132a can be automatically or manually activated. The excitation light source 131a, the sample container 110, and the detector 132a are aligned in an optical system such that the detector 132b receives or captures images from the sample container 110. The optical module 130 can include a detector capable of detecting other wavelengths in accordance with the experimental requirements that need to be performed.

The optical module 130 further includes one or more filters or one or more image elements. The filter filters the fluorescence to a specified wavelength range. The image element captures one or more images located within the container assembly 140. More precisely, the image element captures one or more images of the reagent located within the sample container 110. The position of the image element can receive the fluorescence filtered by the filter, so the image element captures only images in a specific wavelength range. The image element, the filter, the detector 132, the sample container 110, and the excitation light sources 131a and 131b are disposed in the same optical system such that the image element can acquire an image of the sample container 110.

In another embodiment, the detector 132a can be integrated with the excitation light source 131a on the same component, and the detector 132b can also be integrated with the excitation light source 131b on the same component to reduce the detectors. And the power consumption and volume of the excitation source.

In the present disclosure, the photothermal reaction unit 190 can also include one or more container assemblies 140 to house the sample container 110. The heating element 121 and the sample temperature sensor 122 are coupled to the container assembly 140 and are located at the bottom of the container assembly 140 and below the sample container 110. The optical module 130 is coupled to the container assembly 140 and is located on a sidewall of the container assembly 140. The optical module 130 focuses on a transparent area of the sample container 110. The cooling element 123 is located on the outer wall of the container assembly 140. The container assembly 140 is constructed from one or more materials of high thermal conductivity. The high thermal conductivity material can include both metallic and non-metallic materials.

The container assembly 140 further includes at least one opening connected to the slot 141, and the optical module 130 includes excitation light sources 131a, 131b for emitting excitation light, and detectors 132a, 132b for detecting visible light or a fluorescent light. Signal. The container assembly 140 includes the slot 141 for receiving the sample assembly 110 and for connecting the detector 132a, the detector 132b, the excitation light source 131a, the excitation light source 131b, and the heating element 121 to the container assembly 140. Multiple openings on the top. In FIG. 1, only the openings 142a and 142b for connecting the detector 132b and the excitation light source 131b to the container assembly 140 are depicted. The container assembly 140 can have one or more openings to which a plurality of excitation light sources or detectors are coupled. If the excitation light sources 131a, 131b and the detectors 132a, 132b are integrated on the same component, only one opening is required on the container assembly 140 to connect the excitation light source to the container assembly 140.

The optical module 130 is fixed to the container assembly 140. When the photothermal reaction is performed, the optical module 130 is fixed relative to the container assembly 140, so the distance between the optical module 130 and the sample container 110 does not change. The fixed position between the optical module 130 and the container assembly 140 is such that the photothermal reaction unit 10 can be used outside the laboratory or can be carried because the photothermal reaction unit 10 is moved from one location to another. The optical module 130 does not need to be corrected again.

The temperature control module 120 can also include a container assembly temperature sensor 124. In the present embodiment, the container assembly temperature sensor 124 directly contacts the container assembly 140 and is placed beside the container assembly 140 to detect the temperature of the container assembly 140. The container assembly temperature sensor 124 transmits temperature information to the first temperature program. Preferably, the container assembly temperature sensor 124 is a thermocouple. In another embodiment, the container assembly temperature sensor 124 can detect the temperature therein without contacting the container assembly 140. Preferably, the container assembly temperature sensor 124 is an infrared temperature sensor.

In another embodiment, the container assembly temperature sensor 124 can also be integrated with the cooling element 123 on the same component to reduce the power consumption and volume of the cooling element and the container assembly temperature sensor.

Referring to FIG. 2, the present disclosure provides an optical system between the excitation light source, the detector and the sample container, and in the photothermal reaction analyzer or the photothermal reaction analysis unit. The optical system formed by the excitation light source 131, the sample container 110 and the detector 132 can optimize the process of signal detection. An angle a between the excitation source 131, the sample container 110, and the detector 132 has been minimized such that an image of the reagent in the sample container 110 is captured by the detector 132. No distortion. The angle α can be between 0 and 60 degrees. If the angle α is smaller, the image quality of the reagent in the sample container 110 is better. Preferably, the angle a should be as close as possible to 0 degrees.

Referring to FIG. 3, the present disclosure provides another photothermal reaction analyzer 20. The photothermal reaction analyzer 20 includes a plurality of container assemblies 140, a container assembly receiving device 150, a plurality of temperature control modules (not shown), and a plurality of optical modules 130. The container assembly receiving device 150 includes a plurality of recesses 151, and each recess 151 houses a set of the container assemblies 140. Each sample container 110 is housed in the tank 141 to accommodate the reagents required to perform a photothermal reaction. The temperature control module (not shown) is coupled to the container assembly receiving device 150 for controlling a third temperature program corresponding to the container assembly receiving device 150, and controlling a second temperature program corresponding to the sample container . Each optical module 130 is coupled to a container assembly 140 for emitting and receiving light. Within each photothermal reaction analyzer 20, the optical module 130 is stationary relative to the container assembly 140.

In FIG. 3, a plurality of container assembly temperature sensors 124 or cooling elements 123 can be located on each container assembly 140, and the positional relationship is similar to that of FIG. 1, wherein the container assembly temperature sensor 124 and the cooling Element 123 is in contact with the container assembly receiving device 150 (not shown). The plurality of cooling elements 123 can also be replaced by a single cooling element that is coupled to the container assembly receiving device 150. The container assembly temperature sensor 124 and the cooling element 123 stabilize the ambient temperature according to a third temperature program. The third temperature program adjusts the temperature of the container assembly receiving device 150. Preferably, the third temperature program is for placing the container assembly receiving device 150 at a constant temperature and is unaffected by the surrounding environment. Therefore, the photothermal reaction in the sample container 110 can be started under a stable condition and is extremely low or unaffected by the surrounding environment. Each container assembly 140 is coupled to a heating element 121 (not shown) and the heating element 121 adjusts the temperature of the sample container 110 in accordance with the second temperature program. Each of the sample containers 110 of Figure 3 can also be simultaneously adjusted by the second temperature program to have different temperatures from each other.

The optical component 130 is secured to the container assembly 140. When the photothermal reaction is performed, the optical module 130 is fixed relative to the container assembly 140, so the distance between the optical module 130 and the sample container 110 does not change. The fixed position between the optical module 130 and the container assembly 140 is such that the photothermal reaction unit 20 can be used outside the laboratory or can be carried because the photothermal reaction unit 20 is moved from one location to another. The optical module 130 does not need to be corrected again.

Referring to FIG. 4, the present disclosure provides another photothermal reaction analyzer 30. The photothermal reaction analyzer 30 includes a container assembly block 250 including a plurality of slots 251, a plurality of sample containers 110, a temperature control module 120, and a plurality of optical modules 130. Each sample container 110 can be housed within the tank 251 and can contain the reagents required to perform a photothermal reaction. The temperature control module 120 is coupled to the container assembly block 250 to control a fourth temperature program corresponding to the container assembly block 250 and a second temperature program corresponding to the sample container 110. Each optical module 130 is coupled to a slot 251 of the container assembly block 250, and the optical module 130 is stationary relative to the container assembly block 250.

The container assembly block 250 can be integrated with the sample container 110 and the optical module 130 of FIG. The container assembly block 250 includes a plurality of slots 251, and each slot 251 can accommodate the same container 110. The container assembly block 250 further includes a plurality of openings, wherein one opening 252a can accommodate an excitation light source and the other opening 252b can accommodate a detector. A temperature control module 120 can be coupled to the container assembly block 250. The temperature control module coupled to the container assembly block 250 includes a cooling element (not shown), a container assembly block temperature sensor (not shown), and a plurality of sample temperature sensors. 122 and a plurality of heating elements 121. Each of the tank assembly 250, the heating element 121, and the sample temperature sensor 122 are used to adjust the temperature of the sample container 110. The heating element 121 and the sample temperature sensor 122 adjust the temperature of the sample container 110 in accordance with a second temperature program. The cooling element and the container assembly block temperature sensor are coupled to the container assembly block 250 (not shown). A fourth temperature program is used to adjust the temperature of the container assembly block 250. Preferably, the fourth temperature program is for placing the container assembly block 250 at a constant temperature and is unaffected by the surrounding environment. Therefore, the photothermal reaction in the sample container 110 can be started under a stable condition and is extremely low or unaffected by the surrounding environment. The container assembly block 250 is coupled to a heating element 121, and the heating element 121 adjusts the temperature of the sample container 110 in accordance with the second temperature program. Each of the sample containers 110 of Figure 4 can also be simultaneously adjusted by the second temperature program to have different temperatures from each other.

Compared with FIG. 3, the container assembly block 250 has better heat transfer capability by reducing the number of components, and also enables the temperature control module 120 to effectively adjust the container assembly block 250 according to the fourth temperature program. temperature.

The optical component 130 is secured to the container assembly block 250. When the photothermal reaction is performed, the optical module 130 is fixed relative to the container assembly block 250, so the distance between the optical module 130 and the sample container 110 does not change. The fixed position between the optical module 130 and the container assembly block 250 allows the photothermal reaction analyzer 30 to be used outside the laboratory or can be carried because the photothermal reaction analyzer 30 is moved from a location to At another location, the optical module 130 does not need to be corrected again.

Please refer to FIG. 5. The present disclosure provides a control module of the photothermal reaction analyzer. One or more photothermal reaction units 10, photothermal reaction units 20, and photothermal reaction analyzer 30 may include a control module 160. The control module 160 controls the operation of the temperature control module 120 and the optical module 130. The control module 160 includes an input unit 161, a microprocessor 162, and an output unit 163.

The input unit 161 can communicate with the microprocessor 162, the detector 132, the sample temperature sensor 122, and the container assembly temperature sensor 124. The user can input one or more commands to the input unit 161, and the input unit 161 can also obtain information from the detector 132, the sample temperature sensor 122, and the container assembly temperature sensor 124. The input unit 161 can include an input interface and an analog-to-digital signal conversion component. The user can input an instruction through the input interface in the input unit 161. The analog-to-digital signal conversion component can convert and transmit the instruction. Preferably, the input interface can be a button, a stop command, a pause command or any other command that can affect the photothermal reaction analysis unit 10.

The microprocessor 162 can communicate with the input unit 161 and the output unit 163. The microprocessor 162 takes instructions from the input unit 161 and transmits one or more execution instructions to the output unit 163. When the temperature of the container assembly 140 is adjusted, the microprocessor 162 receives the temperature information obtained by the container assembly temperature sensor 124 and transmits an instruction to the cooling element according to: 1) the first temperature program adjustment The temperature of the container assembly 140; 2) the third temperature program adjusts the temperature of the container assembly receiving device 150; or 3) the fourth temperature program adjusts the temperature of the container assembly block 250. When the temperature of the sample container 110 is adjusted, the microprocessor 162 obtains information from the sample temperature sensor 122 and transmits an instruction to the heating element 121 in accordance with the second temperature program. Further, the microprocessor 162 can analyze the information obtained by the detector 132 to obtain an experimental result, and transmit the experimental result to the output unit 163. Preferably, the microprocessor 162 can be a microprocessor unit (MPU), a microcontroller unit (microcontroller unt; MCU, a central processing unit (CPU) or any other data processing. element.

The output unit 163 can communicate with the microprocessor 162, the heating element 121, the excitation light sources 131a and 131b, and the cooling element 123. The output unit 163 can output an execution instruction to the heating element 121, the excitation light sources 131a and 131b, and the cooling element 123. In addition, the output unit 163 can obtain an experimental result from the microprocessor 162 and output the result. The output unit 163 can include a display interface and a digital-to-analog signal conversion component. The display interface is retrieved by the microprocessor 162 and displays the results of the experiment. The digital-analog signal conversion component converts and transmits the experimental result. Preferably, the display interface can be a display screen, a printer or the like.

Further, the control module 160 can also include a communication unit 164 and a memory 165. The communication unit 164 can communicate with the memory 165.

The communication unit 164 can communicate with the microprocessor and an external device. The communication unit 164 is for transmitting information between the photothermal reaction unit 10 and the external device. Preferably, the communication unit 164 may also include one or more infinite communication components, such as a Bluetooth (Blootooth ^® ) component, a WIFI component, or the like. The external device can be a computer device such as a computer, a tablet computer, a smart phone, a notebook computer or the like. Still further, the user can input one or more instructions from the communication unit 164 or input one or more instructions from one or more computer devices in communication with the communication unit 164, and the microprocessor 162 retrieves the Experimental results.

The memory 165 is in communication with the microprocessor 162. The memory 165 can be used to store a workflow or the experimental results from the microprocessor 162. Preferably, the memory 165 can be a random access memory (RAM), a memory card or the like.

It should be noted that the temperature control module 120, the heating element 121, the sample temperature sensor 122, the cooling element 123, the container assembly temperature sensor 124, and the optical module 130 in FIG. The container assembly 140 and the sample container 110 may correspond to elements having the same number in FIG. 1, respectively. The excitation light source 131 is a collection of the excitation light sources 131a and 131b, and the detector 132 is a collection of the detectors 132a and 132b.

Please refer to FIG. 6. The present disclosure provides a housing of the photothermal reaction unit in a photothermal reaction analyzer. The photothermal reaction analyzing unit 10 in FIG. 1 may further include a housing 170 for housing the container assembly receiving device 150 and the control module 160.

Please refer to FIG. 7. The present disclosure provides a flow for performing an immunofluorescence reaction using the above photothermal reaction analyzer. The flow in Figure 7 can be automatically commanded by the photothermal reaction analyzer or manually by the user.

Step 211 is to determine whether to heat the reagent in the sample container. If the reagent is necessary to be heated, the microprocessor instructs the photothermal reaction analyzer to perform step 213.

The reagent may include one or more analytes, fluorescent dyes, labels, antibodies or other reagents required for the immunofluorescence reaction or for the polymerase chain reaction. The marker or the fluorescent dye is used to highlight the results of the experiment. The label can specifically bind to the analyte. Preferably, in the immunofluorescence reaction, the label can form a chemical bond with an antibody or the analyte. In the immunofluorescence reaction, the fluorescent dye can form a bond with an antibody or the analyte. The test substance can be one or more proteins, polypeptide chains or nucleic acids taken from one or more biological sources. The biological source includes one or more biological tissues, cells, body fluids, or body fluid derivatives. The biological source can be blood, serum, plasma, slides carrying biological tissue, slides carrying biological cells, or any other biological tissue derivative.

If the reagent needs to be heated, the reagent can include one or more thermogenic reactants that can be illuminated by electromagnetic radiation to produce heat. The reactant may be a transition metal material, and the transition metal material may be a transition metal oxide, a transition metal hydroxide, or a nitride, phosphide or arsenide doped transition metal of a Group III metal or A transition metal oxide, or a cerium oxide doped transition metal, transition metal oxide or transition metal hydroxide. The temperature of the reactants rises when it is irradiated with infrared rays.

Step 212 is to heat the reagent to a specified temperature according to a second temperature program. Preferably, the temperature control module can include a heating element, a cooling element, the same temperature sensor, and a container assembly temperature sensor. The specified temperature in step 212 is directed to the temperature required for the immunofluorescence reaction.

Still further, the 212 step requires electromagnetic radiation to increase the temperature of the reactants. The electromagnetic radiation has a wavelength in the range of 200 kHz to 500 THz. Preferably, the electromagnetic radiation is located in the spectrum of the infrared light.

In another embodiment, the temperature of the reagent can be detected by the photothermal analyzer and the reagent stays at a specified temperature for a specified time interval. The length of the specified time interval can be set according to the reaction time required for the immune reaction.

Step 213 is to excite the fluorescent light in the reagent by the excitation light emitted by an excitation light source. The excitation light to be emitted may be determined according to the type of the fluorescent dye in the reagent: if the fluorescent dye in the reagent is SYBR, a blue excitation light having a wavelength range of 450 to 495 nm may be used; If the fluorescent dye in the reagent is ROX, a green light excitation light having a wavelength range of 495 to 570 nm can be used.

Step 214 is to use the detector to detect the fluorescent light emitted by the reagent. According to the type of the fluorescent reagent in the reagent, which kind of fluorescence needs to be detected: if the fluorescent dye in the reagent is SYBR, the green fluorescent light can be detected; if the fluorescent light in the reagent When the dye is ROX, the red fluorescent light can be detected.

If there is no fluorescent dye in the sample container, the reaction time may be longer. Figure 8 is an image of a fluorescent signal in the same container in accordance with an embodiment of the present disclosure. The fluorescent signal can be captured by the detector and accumulated in the reaction. Column B in Figure 8 is the fluorescent signal in the sample container measured by the photodiode in the detector, and column A in Figure 8 is the sample captured by the image element in the detector. The image of the container. In the upper half of Fig. 8, the SYBR is not present in the reagent in the sample container, so the image captured by the image element in the detector has no green fluorescence emitted by the SYBR, as shown in Fig. 8 The top half of the bar is shown. The upper half of the column of Figure 8B shows that the reaction time of the immune response is longer when no green fluorescence from the SYBR in the sample container is detected. In the lower half of Figure 8, the SYBR is in the reagent in the sample container, so the image captured by the image element in the detector has green fluorescence emitted by the SYBR, as shown in column A of Figure 8. Shown in half. The lower half of the column of Figure 8B shows that when the green fluorescence emitted by the SYBR in the sample container is detected, the reaction time of the immune reaction is shorter than that of the reaction with SYBR.

In another embodiment, the step 211 further comprises: filling the reagent into the sample container and placing the sample container in the container assembly of the photothermal reaction analyzer.

In another embodiment, the step 211 further includes the following steps 2111 to 2113:

Step 2111 is to enter one or more instructions by the user. This command can be a start command. Preferably, the step 2111 may include the user entering one or more instructions to the photothermal reaction analyzer. The instructions can be a plurality of methods including a workflow and implemented in a computer. The memory in the microprocessor can store the computer implemented method described above.

Step 2112 is to determine if the reagent needs to be heated. If heating is necessary, the microprocessor can transmit one or more commands to the temperature control module and the optical module. If heating is not necessary, the microprocessor can also transmit one or more commands to the temperature control module and the optical module. Preferably, the optical module can include one or more detectors and an excitation light source.

Preferably, the computer implemented method can be loaded by the memory in the microprocessor to determine if the reagent needs to be heated.

Preferably, the microprocessor transmits one or more commands to the temperature control module and the optical module through an output unit.

In another embodiment, the step 214 further includes the following steps 2141 through 2143:

Step 2141 is to obtain one or more fluorescent information of the reagent by the microprocessor.

Step 2142 is to analyze the fluorescent information by the microprocessor and obtain experimental results.

Step 2143 is to determine if the reaction has been completed. If the reaction is completed, the microprocessor can send a stop command to the temperature control module and the optical module. Preferably, the step 2143 can also include storing the experimental results to the memory. In addition, the experimental results can also be transmitted to an external device.

An embodiment of the present disclosure further provides a method for performing a polymerase chain reaction using the photothermal reaction analyzer described above, comprising:

Step 221 is to heat the reagent in the sample container with the temperature control module according to the second temperature program.

The reagent may include one or more polymerases, deoxynucleoside triphosphates, primers, template sequences, or any other reagent required to perform a polymerase chain reaction or an instant polymerase chain reaction.

The reagent can include one or more thermogenic reactants. The heat generating reactant can be irradiated with electromagnetic waves to generate heat. The reactant may be a transition metal material, and the transition metal material may be a transition metal oxide, a transition metal hydroxide, or a nitride, phosphide or arsenide doped transition metal of a Group III metal or A transition metal oxide, or a cerium oxide doped transition metal, transition metal oxide or transition metal hydroxide. The temperature of the reactants rises when it is irradiated with infrared rays.

The second temperature program instructs the control module to cause the heating element to emit electromagnetic radiation to raise the temperature of the reagent to one or more specified temperatures. The electromagnetic radiation has a wavelength in the range of 200 kHz to 500 THz. Preferably, the electromagnetic radiation is in the wavelength range of the infrared light.

The specified temperature is set according to the second temperature program and is the temperature required for each stage of the polymerase chain reaction.

In another embodiment, the reagent temperature is sensed and the reagent temperature is maintained at a specified temperature for the specified time interval. The specified time interval was developed based on experimental criteria for polymerase chain reaction.

In another embodiment, the step 221 further comprises: filling the reagent container into the sample container and placing the sample container into the container assembly of the photothermal reaction analyzer.

In another embodiment, the step 221 further includes the following steps 2211 to 2213:

Step 2211 is to enter one or more instructions by the user. This command can be a start command. Preferably, the step 2111 may include the user entering one or more instructions to the photothermal reaction analyzer. The instructions can be a plurality of methods including a workflow and implemented in a computer. The memory in the microprocessor can store the computer implemented method described above.

2212 is a microprocessor sends a heating command to the temperature control module. The computer implemented method can be loaded by the memory in the microprocessor to determine if the reagent needs to be heated. Preferably, the microprocessor transmits one or more commands to the temperature control module through an output unit.

In another embodiment, the step 221 further includes the following steps 222 to 223:

Step 222 is to determine if the reaction has been completed. If the reaction is completed, the microprocessor can send a stop command to the temperature control module. Preferably, the step 2143 may also include displaying the completion signal or transmitting the experimental result to an external device.

Referring to Figure 9, the present disclosure provides a method of performing an instant polymerase chain reaction using the photothermal reaction analyzer described above. The method includes:

Step 231 is to heat the reagent in the sample container with the temperature control module.

The reagent may include one or more polymerases, deoxynucleoside triphosphates, primers, probes, template sequences, fluorescent stains, or any other reagent required to perform an immediate polymerase chain reaction. The fluorescent dye can form a bond with the probe.

The reagent can include one or more thermogenic reactants. The heat generating reactant can be irradiated with electromagnetic radiation to generate heat. The reactant may be a transition metal material, and the transition metal material may be a transition metal oxide, a transition metal hydroxide, or a nitride, phosphide or arsenide doped transition metal of a Group III metal or A transition metal oxide, or a cerium oxide doped transition metal, transition metal oxide or transition metal hydroxide. The temperature of the reactants rises when it is irradiated with infrared rays.

Additionally, electromagnetic radiation is necessary in order to raise the temperature of the reagent to one or more specified temperatures. The electromagnetic radiation has a wavelength in the range of 200 kHz to 500 THz. Preferably, the electromagnetic radiation is in the wavelength range of the infrared light. The specified temperature is set according to the temperature required for each stage of the instant polymerase chain reaction.

In another embodiment, the reagent temperature is sensed and the reagent temperature is maintained at a specified temperature for the specified time interval. The specified time interval is based on the time required for each phase of the instant polymerase chain reaction.

Step 232 is to excite the fluorescent light in the reagent by the excitation light emitted by an excitation light source. The excitation light to be emitted may be determined according to the type of the fluorescent dye in the reagent: if the fluorescent dye in the reagent is SYBR, a blue excitation light having a wavelength range of 450 to 495 nm may be used; If the fluorescent dye in the reagent is ROX, a green light excitation light having a wavelength range of 495 to 570 nm can be used.

Step 233 is to use the detector to detect the fluorescent light emitted by the reagent. According to the type of the fluorescent reagent in the reagent, which kind of fluorescence needs to be detected: if the fluorescent dye in the reagent is SYBR, the green fluorescent light can be detected; if the fluorescent light in the reagent When the dye is ROX, the red fluorescent light can be detected.

In another embodiment, the step 231 further comprises: filling the reagent into the sample container and placing the sample container in the container assembly of the photothermal reaction analyzer.

In another embodiment, the step 231 further includes the following steps 2311 to 2313:

Step 2311 is to enter one or more instructions by the user. This command can be a start command. Preferably, step 2311 can include the user inputting one or more instructions to the photothermal reaction analyzer. The instructions can be a plurality of methods including a workflow and implemented in a computer. The memory in the microprocessor can store the computer implemented method described above.

Step 2312 is for the microprocessor to transmit one or more commands to the temperature control module and the optical module. Preferably, the computer implemented method can be loaded by a memory in the microprocessor where it is determined whether the reagent needs to be heated.

Preferably, the microprocessor transmits one or more commands to the temperature control module and the optical module through an output unit.

In another embodiment, the step 233 further includes the following steps 2331 to 2333:

Step 2331 is to obtain one or more fluorescent information of the reagent by the microprocessor.

Step 2332 is to analyze the fluorescent information by the microprocessor and obtain experimental results.

Step 2333 is to determine if the reaction has been completed. If the reaction is completed, the microprocessor can send a stop command to the temperature control module and the optical module.

The experimental results are then stored in the memory. Alternatively, the results of the experiment can be displayed on or transmitted by an external device.

In summary, the creation meets the requirements of the invention patent, and the patent application is filed according to law. However, the above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited to the above embodiments, and those skilled in the art will be equivalently modified or changed according to the spirit of the present invention. It should be covered by the following patent application.

10,20‧‧‧Photothermal reaction unit

110‧‧‧ sample container

120‧‧‧temperature control module

121‧‧‧ heating element

122‧‧‧sample temperature sensor

123‧‧‧Cooling element

124‧‧‧Container component temperature sensor

130‧‧‧Optical module

131,131a,131b‧‧‧Excitation source

132, 132a, 132b‧‧‧Detector

140‧‧‧Container components

141,251‧‧‧ slots

142a, 142b, 252a, 252b‧‧‧ openings

150‧‧‧Container assembly receiving device

151‧‧‧ recess

30‧‧‧Photothermal Reaction Analyzer

250‧‧‧Container module block

160‧‧‧Control Module

161‧‧‧ input unit

162‧‧‧Microprocessor

163‧‧‧Output unit

164‧‧‧Communication unit

165‧‧‧ memory

170‧‧‧Shell

The description will be better understood from the following description in conjunction with the accompanying drawings in which: FIG. 1 is an exploded view of a photothermal reaction analyzer structure consistent with an embodiment of the present disclosure. 2 is a schematic diagram showing the optical configuration between an excitation light source, an identical container, and a fluorescent detecting element in accordance with an embodiment of the present disclosure. 3 is an external view of a container assembly receiving device in the photothermal reaction analyzer of FIG. 1. 4 is an exploded view of another photothermal reaction analyzer configuration consistent with an embodiment of the present disclosure. Figure 5 is a flow chart showing the functional relationship between the components of the photothermal reaction analyzer of Figure 1. 6 is a schematic view of a housing of a photothermal reaction analyzer in accordance with an embodiment of the present disclosure. 7 is a flow chart of performing an immunofluorescence reaction using a photothermal reaction analyzer in accordance with an embodiment of the present disclosure. Figure 8 is an experimental result of the immunofluorescence reaction of Figure 7. 9 is a flow chart of a method for performing a polymerase chain reaction using a photothermal reaction analyzer in accordance with an embodiment of the present disclosure.

Claims (54)

A photothermal reaction analyzer comprising: a plurality of photothermal reaction units, wherein each photothermal reaction unit comprises: a container assembly comprising a trough; a sample container removably housed in the trough and capable of accommodating execution a reagent required for photothermal reaction; a temperature control module coupled to the container assembly, and the temperature control module controls a first temperature program corresponding to the container assembly and a corresponding one to the sample container a second temperature program; and an optical module coupled to the container assembly and capable of emitting or receiving light, wherein the optical module is stationary relative to the container assembly. The photothermal reaction analyzer of claim 1, wherein the container assembly further comprises at least one opening, the opening being in communication with the slot, and an optical module is coupled to the container assembly through the opening. The photothermal reaction analyzer of claim 1, wherein the optical module is configured to emit a blue excitation light, a green excitation light, an orange excitation light, a red excitation light, or a combination thereof. The photothermal reaction analyzer of claim 1, wherein the optical module is configured to detect a green fluorescent light, a cyan fluorescent light, an orange fluorescent light, a red fluorescent light, or a combination thereof. The photothermal reaction analyzer of claim 4, wherein the optical module is further configured to filter the fluorescence to a wavelength range. The photothermal reaction analyzer of claim 2, wherein the temperature control module comprises: a heating element, the heating element is coupled to one of the container assemblies through the opening to heat the sample container; and a container assembly temperature sensing a sample temperature sensor; and a cooling element. The photothermal reaction analyzer of claim 6, wherein the heating element is an electromagnetic radiation generator. The photothermal reaction analyzer of claim 1, wherein each of the container assemblies further includes at least two openings, the two openings are in communication with the slots, and each optical module includes at least one excitation light source to emit an excitation light and The at least one detector detects a visible light or a fluorescent signal, and the excitation light source and the detector are respectively coupled to the container assembly through the two openings. The photothermal reaction analyzer of claim 8, wherein the excitation light source comprises a blue excitation light source, a green light excitation light source, an orange light excitation light source or a red light excitation light source. The photothermal reaction analyzer of claim 8, wherein the excitation light source comprises a light emitting diode (LED), a semiconductor laser diode (LD) or a combination thereof. The photothermal reaction analyzer of claim 8, wherein the detector comprises a green fluorescent detector, a cyan fluorescence detector, an orange fluorescent detector or a red firefly. Light detector. The photothermal reaction analyzer of claim 8, wherein the detector comprises a photodiode (PD), an avalanche photodiode (APD), and a photomultiplier (photomultiplier). Tube; PMT), a silicon photomultiplier (SIPM) or a combination thereof. The photothermal reaction analyzer of claim 8, wherein the optical module further comprises a filter disposed between the excitation light source and the detector to filter the fluorescence to a wavelength range. A photothermal reaction analyzer according to claim 8 wherein the detector comprises one or more image elements to capture at least one image of the inside of the container assembly. The photothermal reaction analyzer of claim 8, wherein each temperature control module comprises: a heating element, the heating element is coupled to one of the container assemblies through the openings to heat the same container; a detector; a sample temperature sensor; and a cooling element. A photothermal reaction analyzer according to claim 8 wherein the heating element is an electromagnetic radiation generator. The photothermal reaction analyzer of claim 1, wherein the photothermal reaction performed by the photothermal reaction analyzer is an immunofluorescence reaction and an isothermal polymerase chain reaction; Isothermal PCR), a reverse-transcriptase PCR, a multiplex PCR, a digital polymerase chain reaction (digital PCR), a traditional polymerase chain Lock reaction or an instant polymerase chain reaction (real-time PCR). The photothermal reaction analyzer of claim 1, further comprising a control module for controlling the temperature control module and the optical module. A photothermal reaction analyzer comprising: a plurality of container assemblies, each container assembly comprising a slot; a container assembly receiving device, the container assembly receiving device comprising a plurality of recesses, and each recess accommodating the container assembly; a plurality of sample containers, each of which can be accommodated in the tank and can accommodate reagents required to perform a photothermal reaction; a temperature control module, the temperature control module and the container assembly are coupled, and the temperature control The module controls a third temperature program corresponding to the container assembly receiving device and a second temperature program corresponding to the sample container; and a plurality of optical modules, each optical module and the container assembly are coupled and can be issued or Receiving light, wherein the optical module is stationary relative to the container assembly. The photothermal reaction analyzer of claim 19, wherein the container assembly further comprises at least one opening, the opening being in communication with the slot, and an optical module is coupled to the container assembly through the opening. The photothermal reaction analyzer of claim 19, wherein the optical module is configured to emit a blue excitation light, a green excitation light, an orange excitation light, a red excitation light, or a combination thereof. The photothermal reaction analyzer of claim 19, wherein the optical module is configured to detect a green fluorescent light, a cyan fluorescent light, an orange fluorescent light, a red fluorescent light, or a combination thereof. The photothermal reaction analyzer of claim 22, wherein the optical module is further configured to filter the fluorescence to a wavelength range. The photothermal reaction analyzer of claim 20, wherein the temperature control module comprises: a heating element, the heating element is coupled to one of the container assemblies through the opening to heat the sample container; and a container assembly temperature sensing a sample temperature sensor; and a cooling element. The photothermal reaction analyzer of claim 24, wherein the heating element is an electromagnetic radiation generator. The photothermal reaction analyzer of claim 19, wherein each of the container assemblies further comprises at least two openings, the two openings are in communication with the slots, and each optical module includes at least one excitation light source to emit an excitation light and At least one detector detects a visible light or a fluorescent signal, and the excitation light source and the detector are respectively coupled to the container assembly through the two openings. The photothermal reaction analyzer of claim 26, wherein the excitation light source comprises a blue excitation light source, a green light excitation light source, a orange light excitation light source or a red light excitation light source. The photothermal reaction analyzer of claim 26, wherein the excitation light source comprises a light emitting diode (LED), a semiconductor laser diode (LD) or a combination thereof. For example, the photothermal reaction analyzer of claim 26, wherein the detector comprises a green fluorescent detector, a cyan fluorescence detector, an orange fluorescent detector or a red firefly. Light detector. The photothermal reaction analyzer of claim 26, wherein the detector comprises a photodiode (PD), an avalanche photodiode (APD), and a photomultiplier (photomultiplier). Tube; PMT), a silicon photomultiplier (SIPM) or a combination thereof. The photothermal reaction analyzer of claim 26, wherein the optical module further comprises a filter disposed between the excitation light source and the detector to filter the fluorescence to a wavelength range. A photothermal reaction analyzer according to claim 26, wherein the detector comprises one or more image elements to capture at least one image of the inside of the container assembly. The photothermal reaction analyzer of claim 26, wherein the temperature control module comprises: a plurality of heating elements, each of the heating elements being coupled to one of the container assemblies through an opening to heat the same container; a temperature sense of the container assembly a detector; a plurality of sample temperature sensors; and a cooling element. A photothermal reaction analyzer according to claim 33, wherein the heating element is an electromagnetic radiation generator. For example, the photothermal reaction analyzer of claim 19, wherein the photothermal reaction performed by the photothermal reaction analyzer is an immunofluorescence reaction, an isothermal polymerase chain reaction (isothermal polymerase chain reaction; Isothermal PCR), a reverse-transcriptase PCR, a multiplex PCR, a digital polymerase chain reaction (digital PCR), a traditional polymerase chain Lock reaction or an instant polymerase chain reaction (real-time PCR). The photothermal reaction analyzer of claim 19, further comprising a control module for controlling the temperature control module and the optical modules. A photothermal reaction analyzer comprising: a container assembly block comprising a plurality of slots; a plurality of sample containers each of which can be housed in the tank and can accommodate reagents required to perform a photothermal reaction: a temperature control a module, the temperature control module is coupled to the container assembly block, and the temperature control module controls a fourth temperature program corresponding to the container assembly block and a second temperature program corresponding to the sample container; A plurality of optical modules, each optical module being coupled to a slot in the container assembly block, wherein the optical module is stationary relative to the container assembly block. The photothermal reaction analyzer of claim 37, wherein the container assembly block further comprises a plurality of openings, and each opening is in communication with a slot, and an optical module is coupled to the slot through the opening. The photothermal reaction analyzer of claim 37, wherein the optical module is configured to emit a blue excitation light, a green excitation light, an orange excitation light, a red excitation light, or a combination thereof. The photothermal reaction analyzer of claim 37, wherein the optical module is configured to detect a green fluorescent light, a cyan fluorescent light, an orange fluorescent light, a red fluorescent light, or a combination thereof. The photothermal reaction analyzer of claim 40, wherein the optical module is further configured to filter the fluorescence to a wavelength range. The photothermal reaction analyzer of claim 38, wherein the temperature control module comprises: a plurality of heating elements, each of the heating elements being coupled to one of the slots through the opening to heat the sample container; Body temperature sensor; a sample temperature sensor; and a cooling element. The photothermal reaction analyzer of claim 42, wherein the heating element is an electromagnetic radiation generator. The photothermal reaction analyzer of claim 37, wherein the container assembly block further comprises a plurality of openings, and at least two openings are in communication with a slot, and each optical module includes at least one excitation light source to emit an excitation The light and the at least one detector detect a visible light or a fluorescent signal, and the excitation light source and the detector are respectively coupled to a slot in the container assembly block through the two openings. The photothermal reaction analyzer of claim 44, wherein the heating element is an electromagnetic radiation generator. The photothermal reaction analyzer of claim 45, wherein the excitation light source comprises a light emitting diode (LED), a semiconductor laser diode (LD) or a combination thereof. For example, the photothermal reaction analyzer of claim 44, wherein the detector comprises a green fluorescent detector, a cyan fluorescence detector, an orange fluorescent detector or a red firefly. Light detector. The photothermal reaction analyzer of claim 45, wherein the detector comprises a photodiode (PD), an avalanche photodiode (APD), and a photomultiplier tube (a photomultiplier). Photomultiplier tube; PMT), a silicon photomultiplier (SIPM) or a combination thereof. The photothermal reaction analyzer of claim 44, wherein the optical module further comprises a filter disposed between the excitation light source and the detector to filter the fluorescence to a wavelength range. The photothermal reaction analyzer of claim 44, wherein the detector comprises one or more image elements to capture at least one image of the inside of the groove in the block of the container assembly. The photothermal reaction analyzer of claim 44, wherein the temperature control module comprises: a plurality of heating elements, each heating element being coupled to a slot in the container block to heat the sample container; a block temperature sensor; a sample temperature sensor; and a cooling element. The photothermal reaction analyzer of claim 51, wherein the heating element is an electromagnetic radiation generator. The photothermal reaction analyzer of claim 37, wherein the photothermal reaction performed by the photothermal reaction analyzer is an immunofluorescence reaction, an isothermal polymerase chain reaction (isothermal polymerase chain reaction) ;isothermal PCR), a reverse-transcriptase PCR, a multiplex PCR, a digital polymerase chain reaction (digital PCR), a traditional polymerase Chain reaction or a real-time polymerase chain reaction (real-time PCR). The photothermal reaction analyzer of claim 37, further comprising a control module for controlling the temperature control module and the optical modules.