CN113604328B - Amplification detection device and amplification detection method - Google Patents
Amplification detection device and amplification detection method Download PDFInfo
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- CN113604328B CN113604328B CN202110907448.1A CN202110907448A CN113604328B CN 113604328 B CN113604328 B CN 113604328B CN 202110907448 A CN202110907448 A CN 202110907448A CN 113604328 B CN113604328 B CN 113604328B
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- 238000001514 detection method Methods 0.000 title claims abstract description 163
- 230000003321 amplification Effects 0.000 title claims abstract description 78
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 78
- 230000003287 optical effect Effects 0.000 claims abstract description 70
- 238000010438 heat treatment Methods 0.000 claims abstract description 57
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 50
- 238000009434 installation Methods 0.000 claims description 26
- 238000004891 communication Methods 0.000 claims description 23
- 238000011161 development Methods 0.000 abstract description 42
- 102000039446 nucleic acids Human genes 0.000 abstract description 6
- 108020004707 nucleic acids Proteins 0.000 abstract description 6
- 150000007523 nucleic acids Chemical class 0.000 abstract description 6
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- 108020004414 DNA Proteins 0.000 description 3
- 238000012356 Product development Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
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- 238000004458 analytical method Methods 0.000 description 2
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- 238000005516 engineering process Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6491—Measuring fluorescence and transmission; Correcting inner filter effect
Abstract
The present invention relates to an amplification detection apparatus and a nucleic acid detection method, the amplification detection apparatus including: a base assembly; the heating assembly is arranged on the base assembly and is provided with a heating cavity; the fluorescence detection assembly is used for generating a fluorescence detection light path and is detachably arranged on the base assembly; the color development detection assembly is used for generating a color development detection light path and is detachably arranged on the base assembly; the optical signal receiving assembly is arranged on the base assembly; at least one of the fluorescence detection component and the color development detection component is arranged on the base component, and a fluorescence detection light path passes through the heating cavity to reach the optical signal receiving component, or a color development detection light path passes through the heating cavity to reach the optical signal receiving component. The amplification detection device integrates the fluorescence detection component and the color development detection component, is more flexible in use mode, and can be purchased according to the requirements, and is only provided with the fluorescence detection component, the color development detection component or both.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to an amplification detection device and an amplification detection method.
Background
PCR (Polymerase Chain Reaction ) technology is a molecular biological technique that amplifies specific DNA (Deoxyribonucleic Acid ) sequences in vitro. The PCR technology has the characteristics of strong specificity, high sensitivity, low purity requirement, simplicity, convenience and rapidness, and is widely applied to molecular biology detection and analysis.
In order to detect the amplification reaction in real time, the primers added during amplification are usually labeled with isotopes and fluorescein, and the total amount of the product after each cycle can be obtained by detecting the change of fluorescent signals during the amplification reaction as the primers are combined with the DNA template for amplification.
Miniaturization and household use of nucleic acid detection systems are the necessary trends in the development of technical applications. On the one hand, miniaturized point-of-care testing (POCT) devices are in great demand in the areas of customs, side inspection, even food, etc. On the other hand, with the development of economic level, the health awareness of society is increasingly enhanced, and the demands for home detection are also increasing. These all require simplified nucleic acid detection operations, miniaturized (portable) equipment, shorter detection cycles, more accurate interpretation of results, and legibility.
At present, a fluorescence detection structure is generally used for realizing collection of fluorescence signals, the existing fluorescence detection structure generally comprises a light source, two groups of optical filters, two groups of lenses and a detector, excitation light emitted by the light source irradiates a sample to excite the sample to emit fluorescence after being filtered by the optical filters and focused by the lenses, the fluorescence signals reach the detector after being filtered by the optical filters and focused by the lenses, and a data analysis device analyzes and processes the fluorescence signals detected by the detector. Therefore, the fluorescent detection structure in the prior art scheme has complex structure, high requirements on the specification of the light source LED and high cost, and cannot meet the requirements of miniaturization, household use or disposable use of the nucleic acid detection system. And only can realize the detection means of fluorescence detection, the application range is narrower, and the experimental cost is high.
Disclosure of Invention
Based on the above, it is necessary to provide an amplification detection device and an amplification detection method, which can effectively reduce the cost of the device, reduce the size of the device, and meet the demands of miniaturization, household use or disposable use of the amplification detection system, aiming at the problems of complex structure, high cost and single detection means of the existing amplification detection device.
According to one aspect of the present application, there is provided an amplification detection apparatus comprising:
a base assembly;
the heating assembly is arranged on the base assembly and is provided with a heating cavity with an opening at one end;
the optical signal receiving assembly is arranged on the base assembly; and
at least one of a fluorescence detection component for generating a fluorescence detection light path and a color development detection component for generating a color development detection light path, the fluorescence detection light path passing through the heating cavity to the optical signal receiving component, the color development detection light path passing through the heating cavity to the optical signal receiving component.
In one embodiment, the base assembly comprises a main installation cavity with an opening at one end, and a first installation position, a second installation position and a third installation position which are respectively communicated with the main installation cavity;
the heating assembly is detachably limited in the main mounting cavity, the fluorescence detection assembly is detachably mounted in the first mounting position, the optical signal receiving assembly is detachably mounted in the second mounting position, and the color development detection assembly is detachably mounted in the third mounting position.
In one embodiment, the second mounting location and the third mounting location are located on opposite sides of the main mounting cavity in a radial direction, respectively, and the first mounting location is located between the second mounting location and the third mounting location in a circumferential direction of the main mounting cavity.
In one embodiment, the fluorescence detection assembly comprises fluorescence detection light sources, a first light filtering module and a first focusing module which are sequentially arranged at intervals along the radial direction of the main mounting cavity, the first focusing module is located at one side of the first light filtering module, which faces the main mounting cavity, and the fluorescence detection light sources are located at one side of the first light filtering module, which is far away from the main mounting cavity.
In one embodiment, the color detection assembly includes a color detection light source mounted to the third mounting location.
In one embodiment, the optical signal receiving assembly includes a second hub Jiao Mokuai, a second optical filter module and an optical signal sensing module disposed at intervals along a radial direction of the main mounting cavity, the second hub module being located at a side of the second optical filter module facing the main mounting cavity, and the optical signal sensing module being located at a side of the second optical filter module away from the main mounting cavity.
In one embodiment, the heating assembly comprises a heating plate and a heat conducting cylinder, the heating cavity is formed in the heat conducting cylinder, and the heating plate is positioned on one side of the heat conducting cylinder away from the opening end of the heating cavity;
the heat conduction cylinder is provided with a first communication hole, a second communication hole and a third communication hole which are communicated with the heating cavity, the first communication hole is correspondingly arranged with the first installation position, the second communication hole is correspondingly arranged with the second installation position, and the third communication hole is correspondingly arranged with the third installation position.
In one embodiment, the amplification detection apparatus further includes a temperature sensing module, one end of the temperature sensing module is inserted into the heating assembly, and the other end of the temperature sensing module extends out of the base assembly.
In one embodiment, the amplification detection apparatus further includes a control module, where the control module is communicatively connected to the optical signal receiving component and is configured to obtain a detection result of the optical signal receiving component.
In one embodiment, the amplification detection apparatus further comprises a display module communicatively coupled to the control module.
According to another aspect of the present application, there is provided an amplification detection method using the amplification detection apparatus described above, comprising the steps of:
after a sample is put in, acquiring target parameters acquired N times after a target time, wherein the target time is the sum of the time of putting in the sample and a first preset time length;
according to the target parameters acquired for N times, determining N first parameters;
comparing the magnitudes of the N first parameters with a preset threshold value;
and under the condition that the N first parameters are smaller than a preset threshold value, determining that the sample is positive.
In one embodiment, the target parameter is an ADC value of the optical signal receiving component.
In one embodiment, the step of determining N first parameters according to the target parameters of the N acquisitions comprises the steps of:
acquiring the target parameters in a second preset time before the target time;
performing linear fitting by utilizing the target parameters in a second preset time period before the target moment to obtain a linear fitting equation;
and obtaining the first parameter according to the linear fitting equation and the target parameter.
In one embodiment, the step of determining N first parameters according to the target parameters of the N acquisitions comprises the steps of:
the first parameter is equal to the target parameter.
In one embodiment, the step of determining N first parameters according to the target parameters of the N acquisitions comprises the steps of:
acquiring an average value of the target parameter in a second preset time before a target time or the target parameter of the target time;
and calculating the difference value between the target parameter acquired N times after the target moment and the average value or the target parameter at the target moment as the first parameter.
The invention has the following technical effects:
the structure of the device is simplified, the use of expensive lenses and optical filters is reduced, the specification requirement on the light source LED is low, the cost of the device is effectively reduced, the size of the device is reduced, and the requirements of miniaturization, household use or disposable use of a nucleic acid detection system are met;
the amplification detection device can be optionally integrated with the fluorescence detection component and/or the color development detection component, so that the use mode is more flexible, and a user can purchase the amplification detection device which is only provided with the fluorescence detection component, only provided with the color development detection component or provided with the fluorescence detection component and the color development detection component at the same time according to the needs;
the fluorescent detection assembly and the chromogenic detection assembly share the base assembly, the heating assembly and the optical signal receiving assembly, so that the product development period is shortened, and the production cost is reduced;
the photoelectric signal is utilized to rapidly and accurately judge whether the sample is positive or negative, so that weak positive cases which are difficult to identify by naked eyes can be accurately distinguished, the accuracy of detection results is improved, and the use convenience of the amplification detection device is further improved.
Drawings
FIG. 1 is a schematic diagram of an amplification detection apparatus according to an embodiment of the present invention;
FIG. 2 is an exploded view of the amplification detection apparatus shown in FIG. 1;
FIG. 3 is a schematic view showing the structure of a lower base of the amplification detection apparatus shown in FIG. 1;
FIG. 4 is a schematic diagram illustrating the assembly of the lower base, the heating assembly and the fluorescence detection assembly shown in FIG. 3;
fig. 5 is an assembly schematic diagram of the lower base and the color development detection assembly shown in fig. 3.
Reference numerals illustrate:
100. an amplification detection device; 110. a base assembly; 112. a lower base; 1121. a lower center mounting groove; 1122. a first lower mounting groove; 1123. a second lower mounting groove; 1124. a third lower mounting groove; 114. an upper base; 130. a heating assembly; 132. a heat insulating sheet; 134. a heating sheet; 136. a heat conduction tube; 1361. a heating chamber; 150. a fluorescence detection assembly; 152. a fluorescence detection light source; 154. a first filter module; 156. a first focusing module; 170. a color development detection assembly; 180. an optical signal receiving assembly; 181. a second polymer Jiao Mokuai; 183. the second optical filtering module, 185, optical signal sensing module; 190. a temperature sensing module; 200. a reagent tube.
Detailed Description
In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the invention, whereby the invention is not limited to the specific embodiments disclosed below.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present invention, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.
Referring to fig. 1 and 2, an embodiment of the invention provides an amplification detection apparatus 100 for heating a reagent tube 200 containing a sample at a constant temperature to make the sample perform an amplification reaction in the reagent tube 200, and performing fluorescent detection or chromogenic detection on the sample in the amplification reaction.
The amplification detection apparatus 100 includes a control module, a base assembly 110, a heating assembly 130, a light signal receiving assembly 180, and at least one of a fluorescence detection assembly 150 and a color development detection assembly 170. The control module, the heating module 130, the fluorescence detection module 150, the color development detection module 170 and the optical signal receiving module 180 are respectively and detachably installed on the base assembly 110, the heating module 130 is used for heating a sample in the reagent tube 200 under the control of the control module, the fluorescence detection module 150 is used for generating a fluorescence detection light path, the color development detection module 170 is used for generating a color development detection light path, and the optical signal receiving module 180 is used for receiving an optical signal to obtain a detection result.
When only the fluorescent detection assembly 150 is mounted on the base assembly 110, the amplification detection apparatus 100 can perform fluorescent detection on the sample, when only the chromogenic detection assembly 170 is mounted on the base assembly 110, the amplification detection apparatus 100 can perform chromogenic detection on the sample, and when the fluorescent detection assembly 150 and the chromogenic detection assembly 170 are simultaneously mounted on the base assembly 110, the amplification detection apparatus 100 can selectively perform fluorescent detection or chromogenic detection on the sample.
In this way, the amplification detection apparatus 100 may optionally integrate the fluorescence detection assembly 150 and/or the color development detection assembly 170, so that the usage mode is more flexible, and a user may purchase the amplification detection apparatus 100 with only the fluorescence detection assembly 150, only the color development detection assembly 170, or both the fluorescence detection assembly 150 and the color development detection assembly 170 as required, thereby reducing the purchase cost. Because the fluorescent detection assembly 150 and the color development detection assembly 170 share the base assembly 110, the heating assembly 130, and the optical signal receiving assembly 180, the product development cycle is shortened, and the production cost is reduced.
Wherein, the color development detection means that a substance mark and a color development liquid are added to obtain corresponding signals in the amplification process of the sample, and finally detection analysis is performed through the color development detection assembly 170 and the optical signal receiving assembly 180. Because the color development detection assembly 170 has simple structure and low cost, the color development detection assembly 170 can be adopted for detection in the qualitative screening process, and the fluorescent detection assembly 150 is used for fluorescence detection when the detection precision requirement is high, so that the detection cost is saved and the detection accuracy is ensured.
With continued reference to fig. 1 and 2, the base assembly 110 includes a lower base 112 and an upper base 114, the lower base 112 and the upper base 114 being aligned in a first direction to cooperatively define a main mounting cavity, a first mounting location, a second mounting location, and a third mounting location. Wherein, the main installation cavity is located the central point of base subassembly 110, and second installation position and third installation position are located the radial direction of main installation cavity opposite both sides respectively, and in the circumference direction of main installation cavity, first installation position is in between second installation position and the third installation position. As a preferred embodiment, the first mounting location extends along a second direction, and the second mounting location and the third mounting location extend opposite to each other along a third direction, wherein the second direction, the third direction and the first direction are perpendicular to each other.
The heating assembly 130 is limited in the main mounting cavity, the fluorescence detection assembly 150 is detachably mounted at a first mounting position, the optical signal receiving assembly 180 is mounted at a second mounting position, and the color development detection assembly 170 is detachably mounted at a third mounting position.
In this manner, the excitation light emitted from the fluorescence detection unit 150 reaches the heating unit 130, and the fluorescence emitted from the sample in the reagent tube 200 in the heating unit 130 is received by the light signal receiving unit 180. The light emitted from the color detection unit 170 reaches the heating unit 130, and the light emitted from the sample in the reagent tube 200 of the heating unit 130 is received by the light signal receiving unit 180.
Referring to fig. 3, 4 and 5, in one embodiment, the lower base 112 is provided with a lower central mounting groove 1121, a first lower mounting groove 1122, a second lower mounting groove 1123 and a third lower mounting groove 1124. The lower center mounting groove 1121 is located at a center position of the lower base 112, and a cross section of the lower center mounting groove 1121 perpendicular to the first direction is substantially circular. One end of the first lower mounting groove 1122 communicates with the lower center mounting groove 1121, the other end of the first lower mounting groove 1122 extends straight in the second direction in a direction away from the lower center mounting groove 1121, and a cross section of the first lower mounting groove 1122 perpendicular to the first direction is substantially semicircular. One end of the second lower mounting groove 1123 communicates with the lower center mounting groove 1121, the other end of the second lower mounting groove 1123 extends straight in a direction away from the lower center mounting groove 1121 in a third direction, and a cross section of the second lower mounting groove 1123 perpendicular to the third direction is substantially semicircular. One end of the third lower mounting groove 1124 communicates with the lower center mounting groove 1121, the other end of the third lower mounting groove 1124 extends straight in a third direction toward a direction away from the lower center mounting groove 1121, and a cross section of the third lower mounting groove 1124 perpendicular to the third direction is substantially semicircular.
The upper base 114 is provided with an upper central mounting groove, a first upper mounting groove, a second upper mounting groove and a third upper mounting groove, the upper central mounting groove is located at the central position of the lower base 112 and penetrates through the upper base 114 along the first direction, and the cross section of the upper central mounting groove perpendicular to the first direction is approximately circular. One end of the first upper mounting groove is communicated with the upper center mounting groove, the other end of the first upper mounting groove linearly extends along the second direction towards the direction far away from the upper center mounting groove, and the cross section of the first upper mounting groove perpendicular to the second direction is approximately semicircular. One end of the second upper mounting groove is communicated with the upper center mounting groove, the other end of the second upper mounting groove linearly extends along a third direction towards a direction far away from the upper center mounting groove, and the cross section of the second upper mounting groove perpendicular to the third direction is approximately semicircular. One end of the third upper mounting groove is communicated with the upper center mounting groove, the other end of the third upper mounting groove linearly extends along a third direction towards a direction far away from the upper center mounting groove, and the cross section of the third upper mounting groove perpendicular to the third direction is approximately semicircular.
As such, the lower central mounting groove 1121 and the upper central mounting groove together define a main mounting cavity, and an end of the main mounting cavity remote from the lower base 112 in the first direction is open to communicate with the external environment. The first upper mounting groove and the first lower mounting groove 1122 are correspondingly arranged to form together a first mounting position having a substantially circular cross section, the second upper mounting groove and the second lower mounting groove 1123 are correspondingly arranged to form together a second mounting position having a substantially circular cross section, and the third upper mounting groove and the third lower mounting groove 1124 are correspondingly arranged to form together a third mounting position having a substantially circular cross section. It is to be understood that the shapes of the main mounting chamber, the first mounting position, the second mounting position, and the third mounting position are not limited thereto, and may be set as needed.
Further, the inner side walls of the first, second and third mounting positions are further provided with annular limiting grooves, so as to limit the color development detection assembly 170 and the optical signal receiving assembly 180 of the fluorescence detection assembly 150.
The heating assembly 130 includes a heat insulating sheet 132, a heat generating sheet 134, and a heat conductive cylinder 136. The heat insulating sheet 132, the heat generating sheet 134, and the heat conductive tube 136 are stacked in the first direction. The heat conduction cylinder 136 includes a heating chamber 1361 having one end opened, the opening end of the heating chamber 1361 being directed toward the opening end of the main installation chamber to communicate with the external environment, and the reagent tube 200 may be inserted into the heating chamber 1361 from the opening end of the heating chamber 1361. The heat generating plate 134 is formed of a ceramic material and is located on a side of the heat conductive cylinder 136 remote from the open end of the main mounting chamber. The heat insulating sheet 132 is formed of a material such as heat insulating cotton, and is located on a side of the heat generating sheet 134 remote from the heat conductive cylinder 136.
In this manner, the heat insulating sheet 132 is used to prevent heat of the heat generating sheet 134 from being transferred to the base assembly 110, the heat generating sheet 134 is used to generate heat to heat the heat conducting tube 136, and the heat conducting tube 136 conducts the heat generated by the heat generating sheet 134 to the reagent tube 200, thereby performing constant temperature heating on the reagent tube 200.
Further, the sidewall of the heat conductive tube 136 is provided with a first communication hole, a second communication hole and a third communication hole, which are communicated with the heating cavity 1361, the first communication hole is arranged corresponding to the first mounting position, the second communication hole is arranged corresponding to the second mounting position, and the third communication hole is arranged corresponding to the third mounting position.
Thus, the optical signal emitted from the fluorescent detection unit 150 mounted at the first mounting position may enter the heating chamber 1361 through the first communication hole, the fluorescent signal generated from the sample in the reagent tube 200 may reach the optical signal receiving unit 180 mounted at the second mounting position through the second communication hole, the optical signal emitted from the color development detection unit 170 mounted at the third mounting position may enter the heating chamber 1361 through the third communication hole, and the optical signal generated from the sample in the reagent tube 200 may reach the optical signal receiving unit 180 mounted at the second mounting position through the second communication hole.
In some embodiments, the amplification detection apparatus 100 further includes a temperature sensing module 190, one end of the temperature sensing module 190 is inserted into the heat conductive barrel 136 of the heating assembly 130, and the other end of the temperature sensing module 190 extends out of the base assembly 110, so as to obtain the temperature in the heating chamber 1361 in real time, so as to achieve accurate control of the heating temperature.
In some embodiments, the fluorescence detection assembly 150 includes a fluorescence detection light source 152, a first filter module 154, and a first focusing module 156 sequentially arranged at intervals along the second direction, the first focusing module 156 is located on a side of the first filter module 154 near the main mounting cavity, and the fluorescence detection light source 152 is located on a side of the first filter module 154 far from the main mounting cavity. Specifically, the fluorescence detection light source 152 is a light emitting diode for emitting an optical signal; the first filtering module 154 is a filter for filtering unwanted interference light in the filtered signal; the first focusing module 156 is a focusing lens for focusing the scattered light. In this way, the fluorescence detection light source 152, the first light filtering module 154 and the first focusing module 156 are sequentially arranged in the first installation position, the light signal emitted by the light source enters the heating cavity 1361 through the filtering of the first light filtering module 154 and the focusing of the first focusing module 156, and the combination of the first light filtering module 154 and the first focusing module 156 can effectively reduce the requirement on the light source.
The color detection assembly 170 includes a color detection light source mounted at a third mounting location. Specifically, the color-development detection light source is a light emitting diode for emitting a light signal, and the light signal passes through the filter tube in the heating assembly 130 to reach the light signal receiving assembly 180 to form a photoelectric signal.
The optical signal receiving assembly 180 includes a second polymer Jiao Mokuai 181, a second filter module 183 and an optical signal sensing module 185 which are arranged at intervals along the third direction, the second polymer Jiao Mokuai is located at a side of the second filter module 183 facing the main mounting cavity, and the optical signal sensing module 185 is located at a side of the second filter module 183 facing away from the main mounting cavity. Specifically, the second dimer Jiao Mokuai 181 is a focusing lens for focusing scattered light; the second filtering module 183 is a filter for filtering unwanted interference light in the filtered signal, the optical signal sensing module 185 is an optical signal sensor for converting the received optical signal into an optical signal, and the control module can determine whether the sample is positive or negative according to the optical signal.
As such, the optical signal emitted from the sample in the reagent tube 200 reaches the optical signal sensing module 185 through focusing of the second polymer Jiao Mokuai 1541 and filtering of the second filter module 1543 to form an optical-electrical signal.
In some embodiments, the amplification detection apparatus 100 further comprises a display module communicatively coupled to the control module for displaying the amplification detection apparatus 100 and the amplification detection result.
In particular, in one embodiment, the display module includes three color indicator lights, which may be white, orange, and blue, respectively. When the amplification detection apparatus 100 is in the power supply standby state, the blue light is normally on. When the amplification detection apparatus 100 is in a normal detection state, the blue light blinks. When the amplification detection apparatus 100 ends the experiment and the result is negative, the white lamp is normally on. When the amplification detection apparatus 100 ends the experiment and the detection result is positive, the orange light is always on. When the amplification detection apparatus 100 is in a failure state, three lamps flash simultaneously.
The amplification detection apparatus 100 to which the fluorescence detection unit 150 is attached is mounted as follows:
first, the temperature sensing module 190 is inserted into the heat conductive cylinder 136 and the rapidly solidified heat conductive paste is applied to be fixed, and then the heat insulating sheet 132, the heat generating sheet 134, and the heat conductive cylinder 136 into which the temperature sensing module 190 is inserted are sequentially stacked from bottom to top and mounted to the lower center mounting groove 1121 of the lower base 112.
Then, the first focusing module 152, the first filtering module 154, and the fluorescence detection light source 156 of the fluorescence detection assembly 150 are sequentially inserted into the first lower mounting groove 1122, and the second focusing module Jiao Mokuai and the second filtering module 183 of the optical signal receiving assembly 180 are inserted into the second lower mounting groove 1123.
Then, the upper base 114 is covered on the lower base 112, and the two are fixed to each other by a fastener such as a bolt.
Finally, the optical signal sensing module 185 is inserted into the second mounting position and fixed by a fastener such as a bolt, thereby completing the assembly of the amplification detection apparatus 100.
The amplification detection apparatus 100 to which the color development detection module 170 is attached is installed as follows:
first, the temperature sensing module 190 is inserted into the heat conductive cylinder 136 and the rapidly solidified heat conductive paste is applied to be fixed, and then the heat insulator 132, the heating sheet 134, and the heat conductive cylinder 136 into which the temperature sensing module 190 is inserted are sequentially stacked and mounted to the lower center mounting groove 1121 of the lower base 112 from bottom to top.
Then, the color development detecting assembly 170 is mounted in the third lower mounting groove 1124, and the second hub Jiao Mokuai 181 of the optical signal receiving assembly 180 and the second filter module 183 are fitted into the second lower mounting groove 1123.
Then, the upper base 114 is covered on the lower base 112, and the two are fixed to each other by a fastener such as a bolt.
Finally, the optical signal sensing module 185 is inserted into the second mounting position and fixed by a fastener such as a bolt, thereby completing the assembly of the amplification detection apparatus 100.
The amplification detection apparatus 100, in which the optical signal receiving unit 180 and the color development detection unit 170 are simultaneously installed, is installed as follows:
first, the temperature sensing module 190 is inserted into the heat conductive cylinder 136 and the rapidly solidified heat conductive paste is applied to be fixed, and then the heat insulating sheet 132, the heat generating sheet 134, and the heat conductive cylinder 136 into which the temperature sensing module 190 is inserted are sequentially stacked from bottom to top and mounted to the lower center mounting groove 1121 of the lower base 112.
Then, the first focusing module 154, the first filtering module 154, and the fluorescence detection light source 156 of the fluorescence detection assembly 150 are sequentially fitted into the first lower mounting groove 1122, the color development detection assembly 170 is mounted in the third lower mounting groove 1124, and the second dimer Jiao Mokuai and the second filtering module 183 of the optical signal receiving assembly 180 are fitted into the second lower mounting groove 1123.
Then, the upper base 114 is covered on the lower base 112, and the two are fixed to each other by a fastener such as a bolt.
Finally, the optical signal sensing module 185 is inserted into the second mounting position and fixed by a fastener such as a bolt, thereby completing the assembly of the amplification detection apparatus 100.
The amplification detection apparatus 100 integrates the switchable fluorescence detection unit 150 and the color development detection unit 170, so that fluorescence detection and color development detection can be performed as needed, thereby increasing the application range of the amplification detection apparatus 100. In addition, since the fluorescence detection unit 150 and the color development unit 170 share a part of the components, there is no need to develop structural members for the fluorescence detection unit 150 and the color development unit 170, thereby reducing the production cost of the amplification detection apparatus 100 and effectively shortening the product development period.
The application also provides an amplification detection method using the amplification detection device, by using the amplification detection method, whether the sample is positive or negative can be rapidly and accurately judged according to the photoelectric signal of the optical signal receiving assembly 180, so that the accuracy of the detection result is improved, and the use convenience of the amplification detection device is improved.
Specifically, the amplification detection method includes the steps of:
s110: after the sample is put in, acquiring target parameters acquired N times after the target time, wherein the target time is the sum of the time of putting in the sample and the first preset time length.
Specifically, the first preset duration is 7min, and the target parameter is an initial sampling analog-to-digital conversion (ADC) value of the optical signal receiving assembly 180.
S120: and determining N first parameters according to the N acquired target parameters.
Specifically, in an embodiment, step S120 includes the following steps:
s1211: and acquiring the target parameters in a second preset time before the target time.
Specifically, the ADC value of the optical signal receiving section 180 within 3 to 7 minutes after the sample is put in is acquired.
S1212: and performing linear fitting by utilizing the target parameters in a second preset time period before the target moment to obtain a linear fitting equation.
Specifically, the linear equation y=kx+b is obtained by performing linear fitting using the ADC values of the optical signal receiving assembly 180 within 3 to 7 minutes after the sample is placed, where y is a theoretical ADC value, x is time, and k and b are both constants.
S1213: and obtaining a first parameter according to the linear fitting equation and the target parameter.
Specifically, using the linear equation y=kx+b, taking x to obtain a theoretical ADC value corresponding to the linear equation y=kx+b, and then calculating a difference Δy between the theoretical ADC value and the corresponding ADC value obtained after the sample is put into the sample for 7min, where the first parameter is a value of y/b.
Specifically, in another embodiment, the step S120 includes the following steps:
s1221: and acquiring an average value of the target parameters in a second preset time before the target time or the target parameters of the target time.
Specifically, the ADC value of the optical signal receiving section 180 within 3 to 7 minutes after the sample is put in is acquired, and then the average value of the ADC values is calculated; or the ADC value of the optical signal receiving section 180 at 7min after the sample is put in is acquired.
S1222: and calculating the difference value between the target parameter acquired N times after the target moment and the average value or the target parameter at the target moment as a first parameter.
Specifically, the difference between the ADC value after 7min of sample placement and the average value or the target parameter at the target time is calculated as the first parameter.
In other embodiments, the first parameter is equal to the target parameter and the threshold is the difference between the mean and standard deviation of the ADC values within 3min to 7min after sample placement, or 90% of the mean.
In the above embodiment, N is 5, and the time interval for each acquisition of the target parameter is 2s. It will be appreciated that in other embodiments, the specific value of N is not limited, and the time interval for each acquisition of the target parameter may be set as desired.
According to the amplification detection method, the ADC value acquired by the optical signal receiving component 180 is compared with the threshold value, so that whether the sample is positive or negative can be accurately and rapidly judged, weak positive cases which are difficult to identify by naked eyes can be accurately distinguished, the accuracy of the detection result is improved, and convenience is brought to the detection of the household nucleic acid.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (13)
1. An amplification detection apparatus, comprising:
the base assembly comprises a main installation cavity with an opening at one end, and a first installation position, a second installation position and a third installation position which are respectively communicated with the main installation cavity;
the heating assembly is detachably limited in the main mounting cavity and comprises a heating piece and a heat conduction barrel, the heating assembly is provided with a heating cavity with one end being opened, the heating piece is positioned on one side of the heat conduction barrel away from the opening end of the heating cavity, the heat conduction barrel is provided with a first communication hole, a second communication hole and a third communication hole which are communicated with the heating cavity, the first communication hole is correspondingly arranged with the first mounting position, the second communication hole is correspondingly arranged with the second mounting position, and the third communication hole is correspondingly arranged with the third mounting position;
the optical signal receiving assembly is detachably arranged at the second installation position; and
the fluorescent detection assembly is used for generating a fluorescent detection light path and the chromogenic detection assembly is used for generating a chromogenic detection light path, the fluorescent detection assembly is detachably arranged at the first installation position, the chromogenic detection assembly is detachably arranged at the third installation position, the fluorescent detection light path passes through the heating cavity to reach the optical signal receiving assembly, and the chromogenic detection light path passes through the heating cavity to reach the optical signal receiving assembly.
2. The amplification detection apparatus of claim 1, wherein the second mounting location and the third mounting location are located on opposite sides of the main mounting chamber in a radial direction, respectively, and the first mounting location is located between the second mounting location and the third mounting location in a circumferential direction of the main mounting chamber.
3. The amplification detection apparatus of claim 1, wherein the fluorescence detection assembly comprises a fluorescence detection light source, a first filter module and a first focusing module sequentially arranged at intervals along a radial direction of the main mounting cavity, the first focusing module is positioned at a side of the first filter module facing the main mounting cavity, and the fluorescence detection light source is positioned at a side of the first filter module away from the main mounting cavity.
4. The amplification detection apparatus of claim 1, wherein the color detection assembly comprises a color detection light source mounted to the third mounting location.
5. The amplification detection apparatus of claim 1, wherein the optical signal receiving assembly comprises a second hub Jiao Mokuai, a second filter module and an optical signal sensing module disposed at intervals along a radial direction of the main mounting cavity, the second hub module being located on a side of the second filter module facing the main mounting cavity, the optical signal sensing module being located on a side of the second filter module facing away from the main mounting cavity.
6. The amplification detection apparatus of claim 1, further comprising a temperature sensing module having one end inserted into the heating assembly and the other end extending beyond the base assembly.
7. The amplification detection apparatus of claim 1, further comprising a control module communicatively coupled to the optical signal receiving assembly for obtaining a detection result of the optical signal receiving assembly.
8. The amplification detection apparatus of claim 7, further comprising a display module communicatively coupled to the control module.
9. An amplification detection method using the amplification detection apparatus according to any one of claims 1 to 8, comprising the steps of:
after a sample is put in, acquiring target parameters acquired N times after a target time, wherein the target time is the sum of the time of putting in the sample and a first preset time length;
determining N first parameters according to the N acquired target parameters;
comparing the magnitudes of the N first parameters with a preset threshold value;
and under the condition that the N first parameters are smaller than a preset threshold value, determining that the sample is positive.
10. The amplification detection method of claim 9, wherein the target parameter is an ADC value of an optical signal receiving component.
11. The amplification detection method of claim 9, wherein the step of determining N of the first parameters from the N of the acquired target parameters comprises the steps of:
acquiring the target parameters in a second preset time before the target time;
performing linear fitting by utilizing the target parameters in a second preset time period before the target moment to obtain a linear fitting equation;
and obtaining the first parameter according to the linear fitting equation and the target parameter.
12. The amplification detection method of claim 9, wherein the step of determining N of the first parameters from the N of the acquired target parameters comprises the steps of:
the first parameter is equal to the target parameter.
13. The amplification detection method of claim 9, wherein the step of determining N of the first parameters from the N of the acquired target parameters comprises the steps of:
acquiring an average value of the target parameter in a second preset time before a target time or the target parameter of the target time;
and calculating the difference value between the target parameter acquired N times after the target moment and the average value or the target parameter at the target moment as the first parameter.
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| CN202110907448.1A CN113604328B (en) | 2021-08-09 | 2021-08-09 | Amplification detection device and amplification detection method |
| PCT/CN2022/110451 WO2023016355A1 (en) | 2021-08-09 | 2022-08-05 | Amplification detection device and amplification detection method |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003144193A (en) * | 2001-11-09 | 2003-05-20 | Mitsubishi Heavy Ind Ltd | Method for detecting microorganism and device for the same |
| JP2014023442A (en) * | 2012-07-25 | 2014-02-06 | Hitachi High-Technologies Corp | Genetic testing device, genetic testing reagent and genetic testing method |
| CN106140338A (en) * | 2016-06-21 | 2016-11-23 | 中国科学院电子学研究所 | Micro-fluidic chip system and apply the method that this system carries out isothermal duplication and detection |
| WO2018119848A1 (en) * | 2016-12-29 | 2018-07-05 | 湖南圣湘生物科技有限公司 | Pcr fluorescence detector |
| WO2018188655A1 (en) * | 2017-04-13 | 2018-10-18 | Riche Biotech Inc. | Photothermal reaction analyzer |
| CN109811038A (en) * | 2019-01-17 | 2019-05-28 | 浙江大学 | A kind of bottom illuminated nucleic acid isothermal amplification detection portable instrument |
| CN109971643A (en) * | 2019-05-08 | 2019-07-05 | 中国人民解放军军事科学院军事医学研究院 | Portable constant temperature augmentation detection instrument |
| CN111269970A (en) * | 2018-12-05 | 2020-06-12 | 浙江大学 | Simple and rapid DNA field visual detection device and method |
| CN112226350A (en) * | 2020-10-28 | 2021-01-15 | 北京贝泰科技有限公司 | Centrifugal nuclear extraction and amplification integrated system and detection method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7373253B2 (en) * | 2002-02-12 | 2008-05-13 | Idaho Technology | Multi-test analysis of real-time nucleic acid amplification |
| JP4141985B2 (en) * | 2004-05-19 | 2008-08-27 | 株式会社日立ハイテクノロジーズ | Spectrofluorometer and sample cell |
| US9096892B1 (en) * | 2011-02-04 | 2015-08-04 | K2 Biomicrosystems, LLC | Nucleic acid amplification and monitoring apparatus |
| US10203284B2 (en) * | 2013-08-26 | 2019-02-12 | Diagenetix, Inc. | Hardware and mobile software for operation of portable instruments for nucleic acid amplification |
| KR20160106898A (en) * | 2015-03-03 | 2016-09-13 | 한국생명공학연구원 | Light detecting apparatus for realtime molecular diagnosis |
-
2021
- 2021-08-09 CN CN202110907448.1A patent/CN113604328B/en active Active
-
2022
- 2022-08-05 WO PCT/CN2022/110451 patent/WO2023016355A1/en unknown
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003144193A (en) * | 2001-11-09 | 2003-05-20 | Mitsubishi Heavy Ind Ltd | Method for detecting microorganism and device for the same |
| JP2014023442A (en) * | 2012-07-25 | 2014-02-06 | Hitachi High-Technologies Corp | Genetic testing device, genetic testing reagent and genetic testing method |
| CN106140338A (en) * | 2016-06-21 | 2016-11-23 | 中国科学院电子学研究所 | Micro-fluidic chip system and apply the method that this system carries out isothermal duplication and detection |
| WO2018119848A1 (en) * | 2016-12-29 | 2018-07-05 | 湖南圣湘生物科技有限公司 | Pcr fluorescence detector |
| WO2018188655A1 (en) * | 2017-04-13 | 2018-10-18 | Riche Biotech Inc. | Photothermal reaction analyzer |
| CN111269970A (en) * | 2018-12-05 | 2020-06-12 | 浙江大学 | Simple and rapid DNA field visual detection device and method |
| CN109811038A (en) * | 2019-01-17 | 2019-05-28 | 浙江大学 | A kind of bottom illuminated nucleic acid isothermal amplification detection portable instrument |
| CN109971643A (en) * | 2019-05-08 | 2019-07-05 | 中国人民解放军军事科学院军事医学研究院 | Portable constant temperature augmentation detection instrument |
| CN112226350A (en) * | 2020-10-28 | 2021-01-15 | 北京贝泰科技有限公司 | Centrifugal nuclear extraction and amplification integrated system and detection method |
Non-Patent Citations (1)
| Title |
|---|
| 功能核酸探针用于ATP传感检测研究;金芬;《中国博士学位论文全文数据库 工程科技Ⅰ辑 (月刊)》;20150915(第9期);B014-113 * |
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| WO2023016355A1 (en) | 2023-02-16 |
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