CN219991588U - Handheld nucleic acid detection equipment - Google Patents
Handheld nucleic acid detection equipment Download PDFInfo
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- CN219991588U CN219991588U CN202320195776.8U CN202320195776U CN219991588U CN 219991588 U CN219991588 U CN 219991588U CN 202320195776 U CN202320195776 U CN 202320195776U CN 219991588 U CN219991588 U CN 219991588U
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- 238000001514 detection method Methods 0.000 title claims abstract description 50
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- 238000003825 pressing Methods 0.000 claims description 17
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- 238000001914 filtration Methods 0.000 claims description 3
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- 238000003199 nucleic acid amplification method Methods 0.000 description 19
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- 239000000376 reactant Substances 0.000 description 2
- 206010001598 Alcohol intolerance Diseases 0.000 description 1
- 241000008904 Betacoronavirus Species 0.000 description 1
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Abstract
The present disclosure provides a handheld nucleic acid detection device, the device comprising a body for being held by a user; the main body is provided with a mounting cavity for accommodating the bearing module, the bearing module is used for accommodating the reaction tube, and the reaction tube is used for accommodating the object to be tested; the bearing module is connected with a heating module, and the heating module is used for adjusting the temperature of the bearing module so as to adjust the temperature of the reaction tube to a first temperature; the main body is connected with an excitation light module, the excitation light module is arranged at the bottom of the bearing module, and the bearing module is provided with an excitation light inlet towards the excitation light module, so that an excitation light signal of the excitation light module irradiates on the reaction tube through the excitation light inlet; the main body is also provided with a signal acquisition module which is used for acquiring fluorescent signals of the fluorescent reaction of the reaction tube under the irradiation of the excitation light signals in real time and sending the fluorescent signals to the mobile terminal so as to acquire detection results.
Description
Technical Field
The present disclosure relates to the field of convenient nucleic acid detection, and in particular, to a handheld nucleic acid detection device.
Background
The rapid and efficient detection of viruses has important significance for timely finding and preventing virus infection, the virus detection needs centralized sampling and centralized treatment, the risk of cross infection is greatly increased, the virus detection instrument in the prior art is generally large in size and inconvenient to carry and use, and the existing detection method cannot finish the virus detection in a home environment, so that the self-detection and rapid finding of viruses are limited.
Disclosure of Invention
The present disclosure provides a handheld nucleic acid detection apparatus to at least solve the above technical problems in the prior art.
According to a first aspect of the present disclosure, there is provided a hand-held nucleic acid detecting apparatus, the apparatus comprising a main body for being held by a user; the main body is provided with a mounting cavity for accommodating the bearing module, the bearing module is used for accommodating the reaction tube, and the reaction tube is used for accommodating the object to be detected; the bearing module is connected with a heating module, and the heating module is used for adjusting the temperature of the bearing module so as to adjust the temperature of the reaction tube to a first temperature; the main body is connected with an excitation light module, the excitation light module is arranged at the bottom of the bearing module, and the bearing module is provided with an excitation light inlet towards the excitation light module so that an excitation light signal of the excitation light module irradiates on the reaction tube through the excitation light inlet; the main body is also provided with a signal acquisition module, and the signal acquisition module is used for acquiring fluorescent signals of the fluorescent reaction of the reaction tube under the irradiation of the excitation light signals in real time and sending the fluorescent signals to the mobile terminal so as to acquire detection results.
In one embodiment, the heating module includes a heating plate, an overheat protection module, and a temperature sensor; the heating plate is arranged on one side of the bearing module and is used for adjusting the temperature of the bearing module so as to enable the reaction tube to be at a first temperature; the temperature sensor is arranged between the bearing module and the heating plate and is used for detecting the temperature of the bearing module; the temperature sensor is connected with an overheat protection module; the overheat protection module is arranged between the bearing module and the heating plate, and is used for enabling the heating plate to stop heating when the temperature obtained by detection of the temperature sensor is higher than a second temperature, and the second temperature is higher than the first temperature.
In one embodiment, the body includes a top cover and a housing, the top cover being rotatably coupled to the housing; when the top cover is abutted to the shell, the top cover and the shell are locked through magnetic attraction.
In one embodiment, the top cover is provided with a reaction tube pressing plate, and the top cover is connected with the reaction tube pressing plate through an elastic piece; when the top cover is abutted to the shell, the elastic piece applies elastic force towards the reaction tube to the reaction tube pressing plate, so that the reaction tube pressing plate is used for pressing the reaction tube to the bearing module.
In an embodiment, the bearing module is provided with an observation hole, and the observation hole is located in the direction of the bearing module towards the signal acquisition module; the signal acquisition module acquires the fluorescent signal through the observation hole.
In one embodiment, the signal acquisition module includes an acquisition sensor and an optical filter; the optical filter is arranged between the acquisition sensor and the observation hole and is used for filtering out the excitation light signals in the observation hole so as to enable the fluorescence signals to pass through; the collecting sensor is used for collecting fluorescent signals corresponding to the reaction tube in real time; the optical filter can be an acrylic plate optical filter with a specified color.
In an embodiment, the apparatus is provided with a control module connected to the heating module for controlling the heating module to adjust the first temperature; the control module is also connected with the excitation light module and is used for controlling the excitation light module to emit excitation light signals so as to irradiate the reaction tube; the control module is connected with the signal acquisition module and is used for controlling the signal acquisition module to acquire fluorescent signals of fluorescent reactions of the reaction tube under the irradiation of the excitation light signals; the control module is connected with mobile terminal equipment and is used for receiving a control instruction from the mobile terminal equipment so as to control the heating module to adjust the first temperature through the control instruction; or, controlling the light emitting module to emit light through the control instruction; or controlling the signal acquisition module to acquire fluorescent signals through the control instruction.
In an embodiment, the collecting sensor at least includes one or more of RGB sensor, photodiode, photoresistor, CMOS sensor.
In one embodiment, the carrier module is provided with at least one reaction chamber; the reaction cavity is used for accommodating the reaction tube; the observation hole is positioned at one side of the reaction cavity and communicated with the reaction cavity so as to observe a reaction tube accommodated in the reaction cavity; the observation holes are trapezoid holes.
According to a second aspect of the present disclosure, there is provided a hand-held nucleic acid detection method comprising: placing a reaction tube containing an object to be detected in the bearing module; according to a detection instruction from the mobile terminal equipment, the control module controls the heating module to heat the bearing module to a first temperature so as to enable the reaction tube to be at the first temperature; according to a detection instruction from the mobile terminal equipment, the control module controls the light emitting module to emit light so that an excitation light signal irradiates the reaction tube through the excitation light inlet; according to a detection instruction from the mobile terminal equipment, the control module controls the signal acquisition module to acquire fluorescent signals of the reaction tube under the irradiation of the excitation light signals, and the fluorescent signals are sent to the mobile terminal to generate a real-time amplification curve; and analyzing according to the fluorescence intensity and the fluorescence intensity threshold value in the real-time amplification curve to obtain a detection result.
The handheld nucleic acid detection device is provided with a main body, wherein the main body can be held by a user and carried; after a reaction tube containing an object to be detected is placed in a bearing module of a main body, a heating module is controlled by a control module to heat the bearing module to a first temperature, so that the reaction tube is at the first temperature; the control module is used for controlling the light emitting module to emit light so that the excitation light signal irradiates the reaction tube through the excitation light inlet; finally, the control module controls the signal acquisition module to acquire fluorescent signals of the reaction tube under the irradiation of the excitation light signals, so that fluorescent signals corresponding to the reaction tube are obtained, and the detection result is determined, so that a user can operate at home by himself, automatic detection can be realized by only putting the reaction tube into the bearing module, and the user can realize detection of various place adaptability through carrying the equipment disclosed by the disclosure, so that the operability is high.
It should be understood that the description in this section is not intended to identify key or critical features of the embodiments of the disclosure, nor is it intended to be used to limit the scope of the disclosure. Other features of the present disclosure will become apparent from the following specification.
Drawings
The above, as well as additional purposes, features, and advantages of exemplary embodiments of the present disclosure will become readily apparent from the following detailed description when read in conjunction with the accompanying drawings. Several embodiments of the present disclosure are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings, in which:
in the drawings, the same or corresponding reference numerals indicate the same or corresponding parts.
FIG. 1 is a schematic diagram showing a structure of a hand-held nucleic acid detecting apparatus according to an embodiment of the present disclosure;
FIG. 2 shows a second schematic structural view of a handheld nucleic acid detecting apparatus according to an embodiment of the present disclosure;
FIG. 3 shows a third schematic structural view of a hand-held nucleic acid detecting apparatus according to an embodiment of the present disclosure;
FIG. 4 shows a schematic diagram of a handheld nucleic acid detection device according to an embodiment of the present disclosure;
fig. 5 shows a schematic diagram of a handheld nucleic acid detecting apparatus according to an embodiment of the present disclosure.
The reference numerals in the figures illustrate:
1. a main body; 11. a mounting cavity; 12. a load bearing module; 13. a heating module; 14. an excitation light module; 15. a signal acquisition module; 16. a control module; 17. a top cover; 18. a housing; 121. an observation hole; 122. a reaction chamber; 123. excitation light population; 131. a heating plate; 132. a temperature sensor; 171. an elastic member; 172. a reaction tube pressing plate; 173. a guide shaft; 174. a hinge; 175. a top plate; 176. a buckle; 181. a mounting plate; 19. and a power supply module.
Detailed Description
In order to make the objects, features and advantages of the present disclosure more comprehensible, the technical solutions in the embodiments of the present disclosure will be clearly described in conjunction with the accompanying drawings in the embodiments of the present disclosure, and it is apparent that the described embodiments are only some embodiments of the present disclosure, but not all embodiments. Based on the embodiments in this disclosure, all other embodiments that a person skilled in the art would obtain without making any inventive effort are within the scope of protection of this disclosure.
FIG. 1 is a schematic diagram showing a structure of a hand-held nucleic acid detecting apparatus according to an embodiment of the present disclosure; FIG. 2 shows a second schematic structural view of a handheld nucleic acid detecting apparatus according to an embodiment of the present disclosure; FIG. 3 shows a third schematic structural view of a hand-held nucleic acid detecting apparatus according to an embodiment of the present disclosure; please refer to fig. 1, 2 and 3;
according to a first aspect of the present disclosure, there is provided a hand-held nucleic acid detecting apparatus, the apparatus comprising a main body 1, the main body 1 being for holding by a user; the main body 1 is provided with a mounting cavity 11 for accommodating a bearing module 12, the bearing module 12 is used for accommodating a reaction tube, and the reaction tube is used for accommodating an object to be tested; the bearing module 12 is connected with a heating module 13, and the heating module 13 is used for adjusting the temperature of the bearing module 12 so as to adjust the temperature of the reaction tube to a first temperature; the main body 1 is connected with an excitation light module 14, the excitation light module 14 is arranged at the bottom of the bearing module 12, and the bearing module 12 is provided with an excitation light inlet towards the excitation light module 14, so that excitation light signals of the excitation light module 14 irradiate on the reaction tube through the excitation light inlet; the main body 1 is also provided with a signal acquisition module 15, and the signal acquisition module 15 is used for acquiring fluorescent signals of the fluorescent reaction of the reaction tube under the irradiation of the excitation light signals in real time and sending the fluorescent signals to the mobile terminal so as to acquire detection results.
The handheld nucleic acid detecting device is provided with a main body 1, wherein the main body 1 can be held by a user and carried; after the reaction tube containing the object to be detected is placed in the bearing module 12 of the main body 1, the heating module 13 is controlled by the control module 16 to heat the bearing module 12 to a first temperature, so that the reaction tube is at the first temperature; the control module 16 controls the light emitting module to emit light so that the excitation light signal irradiates the reaction tube through the excitation light inlet; finally, the control module 16 controls the signal acquisition module 15 to acquire fluorescent signals of the reaction tube under the irradiation of the excitation light signals, so that fluorescent signals corresponding to the reaction tube are obtained, and a detection result is determined, so that a user can operate the reaction tube at home by himself, automatic detection can be realized by only putting the reaction tube into the bearing module 12, and the user can realize detection of various place adaptability through carrying the equipment disclosed by the disclosure, so that the operability is high. Compared with detection test paper, the device provided by the disclosure has higher accuracy.
In the embodiment of the present disclosure, the main body 1 refers to an apparatus main body 1, and the main body 1 is sized to be easily held and carried by a user; the main body 1 is used for carrying the carrying module 12, the carrying module 12 at least comprises a reaction cavity 122, the reaction cavity 122 is used for accommodating a reaction tube, and the shape of the reaction cavity 122 is matched with the shape of the reaction tube, so that the reaction tube can be accommodated. Specifically, the carrier module 12 is a carrier block. The main body 1 is provided with a mounting cavity 11 for accommodating the bearing module 12, the mounting cavity 11 is used for mounting the bearing module 12, specifically, a mounting plate 181 is connected to the main body 1 through a buckle 176, and the mounting plate 181 is used for mounting the bearing module 12. Thereby mounting the carrier module 12 to the main body 1. The bearing module 12 is used for accommodating a reaction tube, and the reaction tube is used for accommodating an object to be tested; the bearing module 12 is connected with a heating module 13, and the heating module 13 is used for adjusting the temperature of the bearing module 12 so as to adjust the temperature of the reaction tube to a first temperature; wherein the first temperature is a temperature required by the reaction provided by the reaction tube; the heating module 13 includes a heating plate 131, an overheat protection module, and a temperature sensor 132; the heating plate 131 is arranged on the side of the carrier module 12, in particular on the side facing away from the signal acquisition module 15. Wherein the heating plate 131 is used for adjusting the temperature of the bearing module 12 so as to enable the reaction tube to be at a first temperature; the heating elements corresponding to the heating plate 131 may be ceramic heating plates, electrothermal films, heating wires, power resistors, semiconductor heating plates, etc. The temperature sensor 132 is disposed between the carrier module 12 and the heating plate 131, and the temperature sensor 132 is used for detecting the temperature of the carrier module 12; the temperature sensor 132 may be a thermistor, platinum resistor, thermocouple, etc.; the temperature sensor 132 is connected with an overheat protection module; the overheat protection module is arranged between the bearing module 12 and the heating plate 131, and is used for stopping heating the heating plate 131 when the temperature obtained by the detection of the temperature sensor 132 is higher than a second temperature, wherein the second temperature is higher than the first temperature. The second temperature refers to the highest temperature at which the reaction chamber 122 can react. The overheat protection module may be an overheat protection circuit, or may be in other fusing manners, so as to block the heating plate 131.
The main body 1 is connected with an excitation light module 14, the excitation light module 14 is arranged at the bottom of the bearing module 12, and the bearing module 12 is provided with an excitation light inlet towards the excitation light module 14, so that excitation light signals of the excitation light module 14 irradiate on the reaction tube through the excitation light inlet; the excitation light module 14 is fixedly arranged at the bottom of the carrier module 12, and specifically, bonding, threaded connection and the like can be adopted. The excitation light module 14 is integrated with a plurality of light emitting units, which can be specifically LED lamp beads; the main body 1 is also provided with a signal acquisition module 15, and the signal acquisition module 15 is used for acquiring fluorescent signals of the fluorescent reaction of the reaction tube under the irradiation of the excitation light signals in real time and sending the fluorescent signals to the mobile terminal so as to acquire detection results. The bearing module 12 is provided with an observation hole 121, and the observation hole 121 is positioned in the direction of the bearing module 12 towards the signal acquisition module 15; the observation hole 121 is positioned on the opposite side of the heating model; the signal acquisition module 15 acquires fluorescent signals through the observation holes 121; the reaction chamber 122 is used for accommodating a reaction tube; the observation hole 121 is positioned at one side of the reaction chamber 122 and is communicated with the reaction chamber 122 to observe a reaction tube accommodated in the reaction chamber 122; the viewing aperture 121 may be a trapezoidal aperture. The signal acquisition module 15 comprises an acquisition sensor and an optical filter; the optical filter is arranged between the acquisition sensor and the observation hole 121, and is used for filtering out the excitation light signals in the observation hole 121 so as to enable the fluorescence signals to pass through; the collection sensor is used for collecting fluorescent signals corresponding to the reaction tube in real time. Wherein a further light barrier may be fitted, the purpose of which is to place the light signal elsewhere than the viewing aperture 121 to influence the viewing result. The optical filter can be an acrylic plate optical filter with a specified color. An orange acrylic plate may be particularly used because the acrylic plate is inexpensive to manufacture, so as to reduce the cost of the apparatus, and the purpose of the filter is to filter out the excitation light signal in the observation hole 121 and pass the fluorescence signal. The acquisition sensor at least comprises one or more of an RGB sensor, a photodiode, a photoresistor and a CMOS sensor. The carrier module 12 is provided with two reaction chambers 122; further, if the system is applied to non-home situations, the system can be lifted to 6, 8 and the like to cope with various working environments.
Further, the device is provided with a control module 16, the control module 16 is used for receiving an operation instruction from the mobile terminal device, and based on the operation instruction, the control module 16 is connected with a heating module 13 and is used for controlling the heating module 13 to adjust the first temperature; the control module 16 is also connected with the excitation light module 14, and is used for controlling the excitation light module 14 to emit excitation light signals so as to irradiate the reaction tube; the control module 16 is connected with the signal acquisition module 15 and is used for controlling the signal acquisition module 15 to acquire fluorescent signals of fluorescent reactions of the reaction tube under the irradiation of the excitation light signals; the control module 16 is connected with the mobile terminal device and is used for receiving a control instruction from the mobile terminal device so as to control the heating module 13 to adjust the first temperature through the control instruction; or controlling the light emitting module to emit light through the control instruction; alternatively, the signal acquisition module 15 is controlled by a control command to perform fluorescence signal acquisition. The control module 16 may be a control circuit for receiving a signal from the mobile terminal and performing an operation corresponding to the signal.
Further, a power module 19, i.e. a power interface, is further disposed on the housing 18, and the power module 19 supplies power to the detection device through the power interface on the side of the housing 18, specifically, supplies power to the heating module 13, the control module 16, the signal acquisition module 15 and the excitation light module 14, and supplies power to all the electronic components in the main body 1.
FIG. 4 shows a schematic diagram of a handheld nucleic acid detection device according to an embodiment of the present disclosure;
fig. 5 shows a schematic diagram of a handheld nucleic acid detecting apparatus according to an embodiment of the present disclosure. Please refer to fig. 4 and fig. 5;
in one embodiment, the body 1 comprises a top cover 17 and a housing 18, the top cover 17 being rotatably connected to the housing 18; when the top cover 17 abuts against the housing 18, the top cover 17 and the housing 18 are locked by magnetic attraction.
In the embodiment of the present disclosure, the main body 1 includes a top cover 17 and a housing 18, and the top cover 17 is rotatably connected to the housing 18, specifically may be connected by using a hinge 174; the top cover 17 and/or the shell 18 are/is provided with a magnetic attraction piece, and when the top cover 17 is abutted against the shell 18, the top cover 17 and the shell 18 are locked through magnetic attraction. The top cover 17 is provided with a reaction tube pressing plate 172, and the top cover 17 is connected with the reaction tube pressing plate 172 through an elastic piece 171; when the top cover 17 abuts against the housing 18, the elastic member 171 applies an elastic force to the reaction tube pressing plate 172 toward the reaction tubes, so that the reaction tube pressing plate 172 is used to press the reaction tubes against the carrier module 12. Specifically, the elastic member 171 is connected to the top cover 17 through a guide shaft 173, and the guide shaft 173 is used to guide the direction of the spring so as to apply a force in the correct direction to the carrier module 12. Further, the top cover 17 may further be provided with a top plate 175, and the top cover 17 is connected to the top plate 175 through a slot.
Providing a specific embodiment, firstly adding a detection sample and a matched reagent into a reaction tube, wherein the detection quantity can be two channels at one time, and the number of channels can be expanded to 8 or 16 channels under the condition of non-family use, putting the added sample test tube into a sample bearing module 12, covering a top cover 17, connecting the top cover 17 with one end of a shell 18 through a hinge 174, and opening and closing the other end through a magnetic switch; after the top cover 17 is covered, the test tube pressing plate in the top cover 17 receives compression counterforce of the spring, the test tube is pressed by the compression counterforce, aerosol leakage caused by forced opening of a test tube port due to heating expansion of gas in the test tube in the reaction is avoided, and the test tube pressing plate is tightly attached to the sample bearing module 12 at the same time, so that heat of the sample bearing module 12 can be conducted to the test tube pressing plate, and a better temperature control effect can be achieved; the control module 16 is divided into three parts, including a heating module 13, an excitation light module 14 and a signal acquisition module 15; the heating module 13 performs heating according to the temperature set by the reaction and the time flow; the excitation light module 14 is matched with the signal acquisition module 15 to perform fluorescence real-time detection; the mobile terminal is in wireless connection with the equipment, and can control the instrument switch; and heating parameters such as heating time, heating temperature and the like of the heating program can be modified so as to be suitable for isothermal amplification reactions at different temperatures, and signal acquisition interval time can be set through a mobile terminal, so that the excitation light module 14 and the signal acquisition module 15 can perform excitation and signal acquisition according to the set signal acquisition program.
According to a second aspect of the present disclosure, there is provided a hand-held nucleic acid detection method, the method comprising: placing a reaction tube containing an object to be measured in the bearing module 12; according to the detection instruction from the mobile terminal device, the control module 16 controls the heating module 13 to heat the bearing module 12 to a first temperature so as to enable the reaction tube to be at the first temperature; according to the detection instruction from the mobile terminal device, the control module 16 controls the light emitting module to emit light so that the excitation light signal irradiates on the reaction tube through the excitation light inlet; according to the detection instruction from the mobile terminal equipment, the control module 16 controls the signal acquisition module 15 to acquire fluorescent signals of the reaction tube under the irradiation of the excitation light signals, and sends the fluorescent signals to the mobile terminal so as to generate a real-time amplification curve; and analyzing according to the fluorescence intensity and the fluorescence intensity threshold value in the real-time amplification curve to obtain a detection result.
According to the handheld nucleic acid detection method, three specific implementation scenarios are provided;
a visual nucleic acid amplification detection assay comprising the steps of:
1. and inputting the information of the person to be detected. The information recording mode can be that the mobile terminal program records the information manually, or the mobile terminal program scans the health code to record the information of the person to be detected; 2. the nucleic acid sampling is performed using a nucleic acid sampling swab. 3. The preparation of the nucleic acid crude extract, immersing a sampling sample swab in the sample preparation for nucleic acid release (cracking and reagent to obtain the nucleic acid crude extract; 4, preparing and loading the reaction solution, dripping the nucleic acid crude extract into a reaction tube containing the nucleic acid amplification freeze-dried reagent, shaking the reaction tube to completely dissolve and mix the freeze-dried reagent, loading the reaction tube loaded with the reagent into a sample bearing module 12; 5, pressing a heating key on a mobile phone in a reaction manner, starting heating by a detector and completing rapid isothermal nucleic acid amplification according to preset temperature and preset time; 6, acquiring a real-time quantitative nucleic acid amplification curve, in the isothermal nucleic acid amplification process, carrying out real-time signal acquisition by utilizing a signal acquisition module 15, simultaneously transmitting data to a mobile terminal through a wireless module to draw a real-time amplification curve; 7, judging and storing and sharing the data acquired in real time through developed apps or small programs at the mobile terminal, judging the detection result by utilizing the fluorescence intensity on the real-time amplification curve and a set fluorescence intensity threshold value, and outputting the detection result.
Alcohol tolerance detection:
the alcohol tolerance of human body is divided into three grades, namely homozygous wild type-alcohol tolerance, heterozygous mutant type-alcohol slight tolerance and homozygous mutant type-alcohol intolerance; when detecting alcohol tolerance, two different reaction systems are required to be configured for one sample, primers are respectively designed for the wild type and the mutant type, and if only the wild type primer reaction system generates amplification, the wild type primer reaction system is proved to be homozygous wild type; if only the mutant primer reaction system is amplified, it is said to be homozygous mutant; if both reaction systems produce amplification, a heterozygous mutant is indicated.
The alcohol tolerance test comprises the following steps: 1. and inputting the information of the person to be detected. 2. Sampling the oral epithelial cells are wiped by a sampling swab, and sampling is completed. 3. The preparation of the nucleic acid crude extract is that a sampling detection sample swab is immersed into nucleic acid for releasing (cracking and reagent, standing for 3-5 min to obtain the nucleic acid crude extract, preparing and loading the reaction liquid, dripping the nucleic acid crude extract into a reaction tube containing a nucleic acid amplification freeze-drying reagent, wherein the freeze-drying reagent comprises a primer, a probe, enzyme and other reactants, shaking to enable the freeze-drying reagent to be completely dissolved and mixed, loading the reaction tube loaded with the reagent into a sample bearing module 12.5, carrying out reaction, wherein the preset temperature is 63 ℃ through a mobile terminal app or a small program, the preset time is 30 min, the signal acquisition interval is once per minute, after the mobile terminal clicking is started, equipment completes isothermal nucleic acid amplification according to the preset temperature and the preset time, 6, judging the result according to the set detection program, and indicating that the reference inspector is homozygous wild if the fluorescent signal exceeds a threshold value, and indicating that the fluorescent signal exceeds the amplification threshold value, and indicating that the inspector is homozygous wild type is heterozygous.
SARS-Cov-2 assay:
SARS-CoV-2 is an enveloped positive-stranded RNA virus, belonging to the genus beta coronavirus. The high-efficiency and rapid detection is of great significance for early discovery and prevention of virus transmission. The present example was designed for the conserved sequences of SARS-CoV-2ORF1a, E gene and N gene, respectively, and used for rapid screening of SARS-CoV-2 and its mutants. SARS-Cov-2 assay comprises the steps of: 1. and inputting the information of the person to be detected. 2. Sampling the nasal/pharyngeal swab is taken with a sampling swab to complete the sampling. 3. The preparation of the nucleic acid crude extract, immersing the sampling sample swab in the nucleic acid for releasing (cracking and reagent, standing for 3-5 minutes to obtain the nucleic acid crude extract, preparing and loading the reaction solution, dripping the nucleic acid crude extract into a reaction tube containing the nucleic acid amplification freeze-drying reagent, wherein the freeze-drying reagent comprises a primer, enzyme and other reactants, shaking to completely dissolve and mix the freeze-drying reagent, loading the reaction tube loaded with the reagent into a sample bearing module 12.5, carrying out the reaction at the preset temperature of 63 ℃ for 30 minutes, and completing isothermal nucleic acid amplification by equipment according to the preset temperature and the preset time after the beginning of clicking at a moving end, carrying out result detection according to the set detection program, and if a fluorescent signal is generated by a reaction system, the amplification curve rises and exceeds a threshold value, SARS-Cov-2 is positive, otherwise, the SARS-Cov-2 is negative.
It should be appreciated that various forms of the flows shown above may be used to reorder, add, or delete steps. For example, the steps recited in the present disclosure may be performed in parallel or sequentially or in a different order, provided that the desired results of the technical solutions of the present disclosure are achieved, and are not limited herein.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present disclosure, the meaning of "a plurality" is two or more, unless explicitly defined otherwise. The foregoing is merely specific embodiments of the disclosure, but the protection scope of the disclosure is not limited thereto, and any person skilled in the art can easily think about changes or substitutions within the technical scope of the disclosure, and it is intended to cover the scope of the disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (9)
1. A hand-held nucleic acid detecting apparatus, characterized in that the apparatus comprises a main body (1), the main body (1) being for being held by a user;
the main body (1) is provided with a mounting cavity (11) for accommodating a bearing module (12), the bearing module (12) is used for accommodating a reaction tube, and the reaction tube is used for accommodating an object to be detected;
the bearing module (12) is connected with a heating module (13), and the heating module (13) is used for adjusting the temperature of the bearing module (12) so as to adjust the temperature of the reaction tube to a first temperature;
the main body (1) is connected with an excitation light module (14), the excitation light module (14) is arranged at the bottom of the bearing module (12), and the bearing module (12) is provided with an excitation light inlet towards the excitation light module (14) so that an excitation light signal of the excitation light module (14) irradiates on the reaction tube through the excitation light inlet;
the main body (1) is also provided with a signal acquisition module (15), and the signal acquisition module (15) is used for acquiring fluorescent signals of fluorescent reaction of the reaction tube under the irradiation of the excitation light signals in real time and sending the fluorescent signals to the mobile terminal so as to acquire detection results.
2. The apparatus of claim 1, wherein the device comprises a plurality of sensors,
the heating module (13) comprises a heating plate (131), an overheat protection module and a temperature sensor (132);
the heating plate (131) is arranged on one side of the bearing module (12) and is used for adjusting the temperature of the bearing module (12) so as to enable the reaction tube to be at a first temperature;
the temperature sensor (132) is arranged between the bearing module (12) and the heating plate (131), and the temperature sensor (132) is used for detecting the temperature of the bearing module (12);
the temperature sensor (132) is connected with an overheat protection module; the overheat protection module is arranged between the bearing module (12) and the heating plate (131), and is used for stopping heating the heating plate (131) when the temperature obtained by detection of the temperature sensor (132) is higher than a second temperature, wherein the second temperature is higher than the first temperature.
3. The apparatus according to claim 1, characterized in that the body (1) comprises a top cover (17) and a housing (18), the top cover (17) being rotatably connected to the housing (18);
when the top cover (17) is abutted against the shell (18), the top cover (17) and the shell (18) are locked through magnetic attraction.
4. A device according to claim 3, characterized in that the top cover (17) is provided with a reaction tube platen (172), the top cover (17) being connected to the reaction tube platen (172) by means of an elastic member (171);
when the top cover (17) is abutted against the shell (18), the elastic piece (171) applies elastic force towards the reaction tube to the reaction tube pressing plate (172), so that the reaction tube pressing plate (172) is used for pressing the reaction tube on the bearing module (12).
5. The device according to claim 1, characterized in that the carrier module (12) is provided with a viewing aperture (121), the viewing aperture (121) being located in the direction of the carrier module (12) towards the signal acquisition module (15); the signal acquisition module (15) acquires the fluorescent signal through the observation hole (121).
6. The apparatus according to claim 1, characterized in that the signal acquisition module (15) comprises an acquisition sensor and a filter;
the optical filter is arranged between the acquisition sensor and the observation hole (121), and is used for filtering out the excitation light signals in the observation hole (121) so as to enable the fluorescence signals to pass through; the collecting sensor is used for collecting fluorescent signals corresponding to the reaction tube in real time;
the optical filter can be an acrylic plate optical filter with a specified color.
7. The apparatus according to claim 1, characterized in that the apparatus is provided with a control module (16), the control module (16) being connected to the heating module (13) for controlling the heating module (13) to adjust the first temperature;
the control module (16) is also connected with the excitation light module (14) and is used for controlling the excitation light module (14) to emit excitation light signals so as to irradiate the reaction tube;
the control module (16) is connected with the signal acquisition module (15) and is used for controlling the signal acquisition module (15) to acquire fluorescent signals of fluorescent reactions of the reaction tube under the irradiation of the excitation light signals;
the control module (16) is connected with mobile terminal equipment and is used for receiving a control instruction from the mobile terminal equipment so as to control the heating module (13) to adjust the first temperature through the control instruction; or, controlling the light emitting module to emit light through the control instruction; or the control instruction controls the signal acquisition module (15) to acquire fluorescent signals.
8. The apparatus of claim 6, wherein the acquisition sensor comprises at least one or more of an RGB sensor, a photodiode, a photoresistor, a CMOS sensor.
9. The apparatus according to claim 5, characterized in that the carrier module (12) is provided with at least one reaction chamber (122); the reaction cavity (122) is used for accommodating the reaction tube;
the observation hole (121) is positioned at one side of the reaction cavity (122) and is communicated with the reaction cavity (122) so as to observe a reaction tube accommodated in the reaction cavity (122); the observation hole (121) is a trapezoid hole.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202320195776.8U CN219991588U (en) | 2023-02-07 | 2023-02-07 | Handheld nucleic acid detection equipment |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202320195776.8U CN219991588U (en) | 2023-02-07 | 2023-02-07 | Handheld nucleic acid detection equipment |
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CN219991588U true CN219991588U (en) | 2023-11-10 |
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CN202320195776.8U Active CN219991588U (en) | 2023-02-07 | 2023-02-07 | Handheld nucleic acid detection equipment |
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CN (1) | CN219991588U (en) |
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