CN210215376U - Portable nucleic acid colorimetric detection device - Google Patents
Portable nucleic acid colorimetric detection device Download PDFInfo
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- CN210215376U CN210215376U CN201920686855.2U CN201920686855U CN210215376U CN 210215376 U CN210215376 U CN 210215376U CN 201920686855 U CN201920686855 U CN 201920686855U CN 210215376 U CN210215376 U CN 210215376U
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Abstract
The utility model relates to a portable nucleic acid colorimetric detection device, which comprises a main control board, a computer control system, an industrial camera, a test tube carrier and an incubation system; the computer control system is in signal connection with the main control board, and the main control board is in signal connection with the industrial camera and the incubation system; the computer control system sends a temperature control signal to the incubation system through the main control panel, so that the nucleic acid sample placed in the incubation system can be subjected to isothermal amplification reaction; the test tube carrier is arranged on one side of the incubation system and is used for bearing the test tube sample after the isothermal amplification reaction; the industrial camera is arranged on the opposite side of the test tube carrier, photographs all test tube samples placed in the test tube carrier according to a control signal sent by the main control board, and the acquired image data is sent to the computer control system through the main control board; and the computer control system analyzes and judges the received image data in real time to obtain a detection result. The utility model discloses can wide application in nucleic acid color comparison field.
Description
Technical Field
The utility model relates to a nucleic acid detects the field, especially relates to a portable nucleic acid color comparison detection device.
Background
Biomedical research is currently progressing from global and cellular levels down to molecular levels. Nucleic acids are an important class of biomolecules within cells, and are involved in regulating most of the functions of cells. Nucleic acid detection, i.e., nucleic acid amplification detection, generally refers to detection techniques that employ amplification of DNA or RNA to screen for a particular gene. Common nucleic acid detection technologies include Polymerase Chain Reaction (PCR), loop-mediated isothermal amplification (LAMP), nucleic acid sequence-dependent amplification (NASBA), Rolling Circle Amplification (RCA), helicase-dependent DNA isothermal amplification (HDA), Recombinase Polymerase Amplification (RPA), and denatured vacuole-mediated Strand Exchange Amplification (SEA), and the mainstream detection methods thereof are fluorescence detection systems.
The fluorescence quantitative PCR detection technology utilizes the accumulation of fluorescence signals to monitor the whole nucleic acid amplification process in real time in the whole process. Fluorescent groups are added into a reaction system, the reaction tube is irradiated by excitation light with different wavelengths, and when a reagent in the reaction tube is excited to a specific wavelength, an optical sensor such as a photosensitive diode, a photomultiplier and the like is used for transmitting a collected fluorescence intensity signal to a computer for real-time data display and analysis. The fluorescence detection uses instruments such as a fluorescence quantitative PCR detector, an isothermal fluorescence detector and the like according to different detection technologies.
Obviously, these instruments require a fluorescence excitation device and a complicated optical path design, and the presence of fluorescent groups in the detection reagent increases the complexity and cost of fluorescence detection. In addition, most of fluorescence detectors in the market are medium-sized and large-sized instruments used for detection in molecular detection laboratories and hospital clinical laboratory, are expensive and inconvenient to carry, and are not suitable for field, rapid and emergency detection in the fields of food safety, animal diseases, paroxysmal diseases and the like, because an experimenter must take a detection sample to a laboratory for detection.
The colorimetric nucleic acid detection method is a method for detecting nucleic acid by using the light absorption value of reaction liquid under visible light, can determine whether a sample to be detected exists by directly observing the color change of amplification liquid before and after reaction by naked eyes, and is also called as nucleic acid visual detection or visual detection method. The nucleic acid colorimetric detection method can obtain qualitative or quantitative and quick primary screening detection results, and is suitable for application scenes of field detection, emergency detection and the like of emergency events. However, the colorimetric nucleic acid detection can only record the detection result through a hand-written report or manual photographing, and certain possibility of manual misjudgment error or tampering of the experimental result exists, so that the application of the colorimetric nucleic acid detection technology in some fields is limited.
Disclosure of Invention
To the problem, the utility model aims at providing a portable nucleic acid color comparison detection device, the device collect nucleic acid detection, judge in an organic whole, and smaller and more exquisite, accurate, quick, convenient, economy can satisfy the instant demand of detecting at various scene.
In order to achieve the purpose, the utility model adopts the following technical proposal: a portable nucleic acid colorimetric detection device comprising: a master control board, a computer control system, an industrial camera and an incubation system; the computer control system is in signal connection with the main control board, and the main control board is in signal connection with the industrial camera and the incubation system; the computer control system sends a temperature control signal to the incubation system through a main control board so that the nucleic acid sample placed in the incubation system can perform an amplification reaction; and the industrial camera photographs the reaction tube placed in the incubation system according to the control signal sent by the main control board, and the acquired image data is sent to the computer control system through the main control board.
Further, the detection device further comprises a test tube carrier, the test tube carrier is arranged on one side of the incubation system, the industrial camera is arranged on the opposite side of the test tube carrier and is used for photographing the reaction tube after the isothermal amplification reaction placed in the test tube carrier according to a control signal sent by a computer control system, and the acquired image data is sent to the computer control system.
Furthermore, the detection device also comprises a printer which is in signal connection with the computer control system and prints the qualitative or quantitative detection result given by the computer control system.
Further, printer, main control board, computer control system, industrial camera, test tube carrier and incubation system integration set up in a box.
Further, a movable cover is arranged on the outer side of the incubation system.
Furthermore, the incubation system comprises a temperature control heating assembly and a plurality of rows of test tube holes, wherein each row of test tube holes are formed in the temperature control heating assembly, and the temperature control heating assembly is in signal connection with the main control panel through serial port communication.
Further, the computer control system is also provided with a USB unit and a wireless communication unit; the USB interface unit is used for receiving picture data after amplification reaction is carried out by adopting other instruments or equipment; the wireless communication unit is used for realizing data transmission with other equipment in a wifi and bluetooth wireless signal mode and uploading or sharing the detection report.
The utility model discloses owing to take above technical scheme, it has following advantage: 1. the utility model discloses an industry camera shoots the sample in the test tube carrier frame to judge image data's negative and positive based on the colorimetry, can gather data formation light absorption curve in real time, differentiate the testing result analysis. 2. The utility model discloses be provided with the printer, can print the testing result of computer control system output, the testing result is more directly perceived. 3. The utility model discloses incubation system can heat and the amplification reaction to a plurality of test tube samples simultaneously, is provided with a plurality of sample holes in the test tube carrier frame simultaneously, has realized the multichannel detection of sample. 4. The utility model discloses incubation system outside is equipped with the upper cover, guarantees that the amplification process avoids reagent pollution problem in an inclosed space. 5. The utility model discloses be provided with the parameter setting module among the computer control system, can control the incubation system according to predetermineeing the parameter after the system starts, and predetermine the parameter and can revise according to actual conditions, it is more simple and convenient to operate, and application scope is wide. 6. The utility model discloses the integrated setting of each part of detection device is in a box, and small in size can arrange the independent packaging in or arrange the quick-witted incasement of examining in, and is more portable, is fit for the witnessed inspections at any time and any place. 7. The utility model discloses still be provided with wireless communication device, can carry out wireless communication with other equipment, the qualitative or quantitative determination result report that will obtain is uploaded or is shared. The utility model discloses based on the colorimetry nucleic acid examines technique soon, collect and detect, judge in an organic whole, accurate, quick, convenient, the economy satisfies the instant demand of detecting of various scene, can wide application in nucleic acid color comparison detection area.
Drawings
FIG. 1 is a schematic structural diagram of the portable nucleic acid colorimetric detection device of the present invention;
the respective symbols in the figure are as follows: 1. a printer; 2. a main control board; 3. a computer control system; 4. an industrial camera; 5. carrying a test tube frame; 6. an incubation system.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and examples.
As shown in fig. 1, the utility model provides a portable nucleic acid colorimetric detection device, it includes: a master control board 2, a computer control system 3, an industrial camera 4, a test tube carrier 5 and an incubation system 6. Wherein, the computer control system 3 is in signal connection with the main control board 2, and the main control board 2 is in signal connection with the industrial camera 4 and the incubation system 6. The computer control system 3 sends a temperature control signal to the incubation system 6 through the main control board 2, so that the nucleic acid sample placed in the incubation system 6 can be subjected to isothermal amplification reaction; the test tube carrier 5 is arranged on one side of the incubation system 6 and is used for carrying the test tube sample after the isothermal amplification reaction; the industrial camera 4 is arranged on the opposite side of the test tube carrier 5 and is used for photographing all test tube samples placed in the test tube carrier 5 according to the control signal sent by the main control board 2, and the acquired image data is sent to the computer control system 3 through the main control board 2; the computer control system 3 is used for analyzing and judging the received image data in real time and giving qualitative or quantitative detection results.
Preferably, the detector further comprises a printer 1, wherein the printer 1 is in signal connection with the computer control system 3 and prints the qualitative or quantitative detection result given by the computer control system 3.
Preferably, printer 1, main control board 2, computer control system 3, industrial camera 4, test tube carrier 5 and incubation system 6 integration set up in a box for the detector small in size, it is more portable, be applicable to the witnessed inspections. More preferably, the incubation system 6 is provided with a removable cover on the outside thereof for ensuring that the amplification process is performed in a closed space, thereby avoiding the problem of reagent contamination.
Preferably, the industrial camera 4 adopts a CCD imaging, the pixels are 200-1000 ten thousand, and a lens without distortion is adopted.
Preferably, the test tube carrier 5 comprises a plurality of sample wells arranged side by side.
Preferably, the incubation system 6 comprises a temperature control heating assembly and a plurality of rows of test tube holes, wherein all the test tube holes are arranged on the temperature control heating assembly and used for placing the PCR reaction tubes filled with the nucleic acid samples, and the temperature control heating assembly is in signal connection with the main control board 2 through serial port communication and heats under the control of the main control board 2.
Preferably, the detection device may be provided without the test tube carrier 5, in which case the industrial camera 4 is arranged below the test tube well of the incubation system 6, the PCR reaction tube placed in the test tube well of the incubation system 6 is photographed according to the control signal sent by the computer control system 3, and the acquired image data is sent to the computer control system 3.
Preferably, the computer control system 3 comprises a user display interface, a nucleic acid detection module and a serial communication unit. The user display interface is used for providing a human-computer interaction interface and displaying the system state in real time; the nucleic acid detection module comprises a parameter configuration unit, an experiment starting unit, an experiment ending unit and a result interpretation unit. Wherein, the parameter configuration unit is used for setting the initial temperature, the reaction temperature and the reaction time of the incubation system 6, the size, the position and the detection type parameters of the reaction tube placed in the test tube carrier 5, the shooting interval of the industrial camera 4 and whether the judgment result needs to be printed or not; after the system is started, the parameter configuration unit sends the initial temperature of the incubation system 6 to the main control board 2 through the serial port communication unit, the main control board 2 controls the incubation system 6 according to the initial temperature, and the incubation system 6 enters a standby state after reaching the initial temperature; the starting experiment unit is used for sending the reaction temperature and the reaction time stored in the parameter configuration unit to the incubation system 6 after an operator puts the sample into the incubation system 6, automatically timing when the incubation system 6 reaches the reaction temperature, and giving a buzzing prompt when the reaction time is reached; the experiment stopping unit is used for confirming by an operator after the experiment is finished, so that the incubation system 6 is automatically closed to cool, or the experiment is stopped at any time in the experiment process; the result judging unit is used for receiving the image data sent by the industrial camera 4 through the main control board 2, analyzing and judging the image data in real time, and printing an obtained qualitative or quantitative detection result through the printer 1.
Preferably, the preset shooting interval of the industrial camera 4 is 10-30s, which is more beneficial to drawing the light absorption curve.
Preferably, the user display interface is further provided with a real-time photographing unit, and an operator clicks the user display interface in real time according to actual needs to photograph in real time in the experiment process.
Preferably, the computer control system is also provided with a USB unit and a wireless communication unit; the USB interface unit is used for receiving picture data after amplification reaction is carried out by other instruments or equipment (such as a PCR instrument, an isothermal detector, a metal bath and the like), and sending the picture data to the result judgment unit for judgment; the wireless communication unit is used for realizing data transmission with other equipment in wireless signal modes such as wifi and Bluetooth.
Preferably, the result judging unit comprises a data receiving module, a data preprocessing module, a standard curve database, a detection result judging module and a data sending module. The data receiving module is used for receiving image data sent by the industrial camera 4 or the USB interface unit; the preprocessing module is used for preprocessing the received image data, converting the image data into gray data and sending the gray data to the detection result judging module; the detection result distinguishing module is used for comparing the received gray data with standard curve data stored in a standard curve database in real time to obtain a corresponding qualitative or quantitative detection result; when the parameter configuration unit sets the result to be printed, the data transmission module transmits the qualitative or quantitative detection result to the printer 1 for printing.
Based on above-mentioned portable nucleic acid color comparison detection device, the utility model also provides a portable nucleic acid color comparison detection device's application method, including following step:
1) after the system is started, the main control board 2 controls the incubation system 6 to enter a standby state according to the initial temperature stored in the computer control system 3; wherein, the preset initial temperature of the medium incubation system 6 of the utility model is set as 50 ℃;
2) an operator puts a reaction tube for experiment into a test tube hole on the incubation system 6 to start the experiment, the main control board 2 controls the incubation system 6 to heat up to reach a preset reaction temperature and then starts timing, and buzzing is prompted when the sample amplification reaction in the reaction tube reaches a preset reaction time;
3) after confirming that the experiment is finished, the operator automatically closes the incubation system 6 to cool down, and transfers the reaction tube after the amplification reaction to the test tube carrier 5;
4) the main control board 2 controls the industrial camera 4 to take a picture of the reaction tube in real time, and the obtained image data is sent to the computer control system 3;
5) the computer control system 3 analyzes and judges the image in real time and gives a qualitative or quantitative detection result, and the result can be output through the printer 1.
The method for analyzing and judging the image in real time by the computer control system 3 comprises the following steps:
5.1) preprocessing image data received at different moments by adopting a color pickup algorithm to obtain gray data of each moment;
5.2) comparing the gray data, the preset threshold and the time of reaching the threshold at each moment with the standard curve data to give a quantitative detection result; comparing the obtained gray data at each moment with a preset threshold value to obtain a positive and negative judgment result at each moment;
specifically, when the gray data at each moment is compared with a preset threshold, the gray data is positive when the gray data is smaller than the preset threshold, and the gray data is negative when the gray data is larger than the preset threshold; the preset threshold range set by the application is 0-60, more preferably 10-50, and particularly can be set under actual conditions, and the preset threshold set by the utility model is 25;
5.3) drawing a light absorption curve according to the gray data acquired at different moments, and comparing the drawn light absorption curve with a standard curve in a standard curve database to obtain a real-time observation result; wherein, the standard curve database is empirical data accumulated through a large number of sample experiments.
Example one
It is right through the specific embodiment below the utility model discloses the colorimetric detection process of detector introduces.
In this example, a synthetic listeria monocytogenes genomic DNA (5'-GTCATTGGAAACTGGAGACTGGAGTGCAGAAGAGGAGAGTGG-3' (SEQ ID No.1)) was detected using a listeria monocytogenes nucleic acid detection kit (isothermal colorimetry) purchased from qingdao alder organisms (cat No. KDC1104), and the reagents 1 to 8 used were genome sample repeat 1, genome sample repeat 2, genome sample repeat 3, genome sample repeat 4, positive control, negative control, empty tube, and empty tube, respectively, in accordance with the requirements of the specification.
After the amplification reaction solution is prepared, the amplification reaction solution is placed in an incubation module of the detector, the reaction temperature of the incubation system is set to 61 ℃, and the 'start test unit' is clicked. After the buzzing reminding is heard (namely, after the amplification reaction of the incubation system at the reaction temperature reaches the preset reaction time), the reaction tube is moved to the test tube carrier frame in the interpretation area, the parameter setting unit is clicked, and the corresponding parameters are selected according to the size and the position of the reaction tube (the parameter value does not need to be changed under the condition that the size of the reaction tube is consistent because the position of the interpretation test tube carrier is fixed, and the result interpretation unit of a main screen can be directly clicked). Selecting the type of the sample to be detected, clicking to photograph and interpret, displaying the interpretation result in real time by a screen, and printing a detection report.
Above-mentioned each embodiment only is used for explaining the utility model discloses, wherein structure, connected mode and the preparation technology etc. of each part all can change to some extent, all are in the utility model discloses equal transform and improvement of going on technical scheme's the basis all should not exclude outside the protection scope of the utility model.
Claims (7)
1. A portable colorimetric nucleic acid detection device, comprising: a master control board, a computer control system, an industrial camera and an incubation system;
the computer control system is in signal connection with the main control board, and the main control board is in signal connection with the industrial camera and the incubation system;
the computer control system sends a temperature control signal to the incubation system through a main control board so that the nucleic acid sample placed in the incubation system can perform an amplification reaction;
and the industrial camera photographs the reaction tube placed in the incubation system according to the control signal sent by the main control board, and the acquired image data is sent to the computer control system through the main control board.
2. A portable colorimetric nucleic acid detection device as claimed in claim 1, wherein: the detection device also comprises a test tube carrier, wherein the test tube carrier is arranged on one side of the incubation system, the industrial camera is arranged on the opposite side of the test tube carrier and is used for photographing a reaction tube which is placed in the test tube carrier after isothermal amplification reaction according to a control signal sent by a computer control system, and the acquired image data is sent to the computer control system.
3. A portable colorimetric nucleic acid detection device as claimed in claim 2, wherein: the detection device also comprises a printer which is in signal connection with the computer control system and prints the qualitative or quantitative detection result given by the computer control system.
4. A portable colorimetric nucleic acid detection device as claimed in claim 3, wherein: the printer, the main control board, the computer control system, the industrial camera, the test tube carrier and the incubation system are integrally arranged in a box body.
5. A portable colorimetric nucleic acid detection device as claimed in claim 4, wherein: the outer side of the incubation system is provided with a movable cover.
6. A portable colorimetric nucleic acid detection device as claimed in claim 1, wherein: the temperature control heating assembly is in signal connection with the main control board through serial port communication.
7. A portable colorimetric nucleic acid detection device as claimed in claim 1, wherein: the computer control system is also provided with a USB unit and a wireless communication unit;
the USB interface unit is used for receiving picture data after amplification reaction is carried out by adopting other instruments or equipment;
the wireless communication unit is used for realizing data transmission with other equipment in a wifi and bluetooth wireless signal mode and uploading or sharing the detection report.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920686855.2U CN210215376U (en) | 2019-05-15 | 2019-05-15 | Portable nucleic acid colorimetric detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920686855.2U CN210215376U (en) | 2019-05-15 | 2019-05-15 | Portable nucleic acid colorimetric detection device |
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| Publication Number | Publication Date |
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| CN210215376U true CN210215376U (en) | 2020-03-31 |
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| CN201920686855.2U Active CN210215376U (en) | 2019-05-15 | 2019-05-15 | Portable nucleic acid colorimetric detection device |
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