CN218860746U - Portable nucleic acid detection equipment and system - Google Patents

Abstract

The present disclosure provides a portable nucleic acid detection apparatus and system, the apparatus comprising a main body; the main body is provided with an installation cavity for installing a bearing module, the bearing module is used for accommodating a reaction tube, and the reaction tube is used for accommodating an object to be measured; the bearing module is connected with a heating module, and the heating module is used for adjusting the temperature of the bearing module so as to adjust the temperature of the reaction tube; the main body is connected with a light-emitting module, the light-emitting module is arranged at the bottom of the bearing module, and the bearing module is provided with a light through hole towards the light-emitting module, so that the emitted light of the light-emitting module irradiates on the reaction tube through the light through hole; the main body is also provided with an observation window, the first side of the bearing module is provided with a first observation hole, and the first observation hole is used for observing the reaction tube; when the bearing module is installed in the installation cavity, the observation window is used for observing the reaction tube through the first observation hole.

Description

Portable nucleic acid detection equipment and system

Technical Field

The present disclosure relates to the field of nucleic acid detection, and in particular, to a portable nucleic acid detection apparatus and system.

Background

How to detect the virus fast and efficiently, thereby timely discovering and preventing the virus infection, and having great significance for epidemic prevention work, while the virus detection instrument in the prior art is generally bulky, inconvenient to carry and use, the virus detection needs centralized sampling, centralized processing and the like, so that the time interval from sampling to obtaining the detection result is long, the detection needs to be carried out in a certified laboratory, corresponding large instruments and well-trained operators are also needed, and the risk of cross infection is greatly increased by fussy steps.

Disclosure of Invention

The present disclosure provides a portable nucleic acid detecting apparatus and system to at least solve the above technical problems in the prior art.

According to a first aspect of the present disclosure, there is provided a portable nucleic acid detecting apparatus comprising a main body; the main body is provided with an installation cavity for installing a bearing module, the bearing module is used for accommodating a reaction tube, and the reaction tube is used for accommodating an object to be measured; the bearing module is connected with a heating module, and the heating module is used for adjusting the temperature of the bearing module so as to adjust the temperature of the reaction tube; the main body is connected with a light-emitting module, the light-emitting module is arranged at the bottom of the bearing module, and the bearing module is provided with a light through hole facing the light-emitting module, so that the emitted light of the light-emitting module irradiates on the reaction tube through the light through hole; the main body is also provided with an observation window, a first observation hole is formed in the first side of the bearing module, and the first observation hole is used for observing the reaction tube; when the bearing module is installed in the installation cavity, the observation window is used for observing the reaction tube through the first observation hole.

In an implementation manner, a plurality of grooves are formed on the bearing module, and the grooves are used for embedding the reaction tubes; the number of the light through holes is consistent with that of the grooves, the light through holes are formed in the bottoms of the grooves, and the light emitting module is fixedly connected to the bottom of the bearing module so that emitted light of the light emitting module can irradiate on the reaction tube through the light through holes; the bearing module further comprises a mounting plate, and the mounting plate is used for being matched with the main body so as to fix the bearing module in the mounting cavity.

In an embodiment, the heating module is connected to a second side of the carrier module, the second side being different from the first side; the heating module comprises a heating element, a temperature sensor and an overheating protection module, the temperature sensor is arranged between the heating element and the bearing module, the heating module is used for adjusting the temperature of the bearing module, and the temperature sensor is used for detecting the temperature of the bearing module; the temperature sensor is connected with an overheating protection module, and the overheating protection module is connected with the heating element; when the temperature detected by the temperature sensor is higher than the specified temperature, the overheating protection module is used for stopping heating of the heating element.

In one embodiment, the temperature sensor comprises at least one or more of a thermistor, a platinum resistor and a thermocouple, and the heating element comprises at least one or more of a ceramic heating plate, an electrothermal film, a heating wire, a power resistor and a semiconductor heating plate.

In an embodiment, the main body includes a cover and a housing, the cover and the housing are rotatably connected, and the housing is used for abutting a bearing module; the cover body comprises a pressing plate, the pressing plate is elastically connected to the cover body through an elastic piece, and the pressing plate is used for pressing the reaction tube in the bearing module under the condition that the cover body covers the shell.

In an embodiment, a magnetic switch is disposed on the cover body, and the magnetic switch is used for cooperating with the housing to open or close the cover body.

In an embodiment, the apparatus is provided with a control module, and the control module is connected with the heating module and is used for controlling the heating module to adjust the temperature of the bearing module; the control module is also connected with the light-emitting module and is used for controlling the light-emitting module to emit light so as to irradiate the reaction tube; the control module is connected with a mobile terminal device and used for receiving a control instruction from the mobile terminal device so as to control the heating module to adjust the temperature of the bearing module through the control instruction, or control the light emitting module to emit light through the control instruction.

In one embodiment, the equipment further comprises a darkroom shell, wherein the darkroom shell can be used for installing the mobile terminal equipment or the camera; and a second observation hole is formed in the darkroom shell and used for being matched with the mobile terminal device or the camera to observe the reaction tube.

In an embodiment, a fixing mechanism is disposed on the darkroom casing, and the fixing mechanism is used for fixing the mobile terminal device, so that the mobile terminal device observes the reaction tube through the second observation hole.

According to a second aspect of the present disclosure, there is provided a portable nucleic acid detection system, the system comprising: a mobile terminal device and any one of the portable nucleic acid detection devices; the mobile terminal equipment is in communication connection with the portable nucleic acid detection equipment so as to cooperate with the portable nucleic acid detection equipment to perform detection operation.

The portable nucleic acid detection equipment and the method thereof place a reaction tube containing an object to be detected in a bearing module; mounting the carrier module in the mounting cavity; according to a detection instruction from the mobile terminal equipment, controlling the heating module to heat the bearing module to a specified temperature and controlling the light-emitting module to emit light so that the emitted light irradiates on the reaction tube through the light-passing hole; make the determinand fully react in the reaction tube through the heating reaction tube, emit the light through light emitting module to make the emission light shine on the reaction tube through the light passing hole, thereby observe the fluorescence reaction of reaction tube under the illumination of emission light and confirm the testing result, this application step is simple, the flow is convenient, can be quick obtain the inspection result of determinand, the simple structure of equipment, light moreover can conveniently carry and the family uses.

It should be understood that the statements in this section are not intended to identify key or critical features of the embodiments of the present disclosure, nor are they intended to limit the scope of the present disclosure. Other features of the present disclosure will become apparent from the following description.

Drawings

The above and other objects, features and advantages of exemplary embodiments of the present disclosure will become readily apparent from the following detailed description, which proceeds with reference to the accompanying drawings. Several embodiments of the present disclosure are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which:

in the drawings, the same or corresponding reference numerals indicate the same or corresponding parts.

FIG. 1 shows a first schematic structural diagram of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure;

FIG. 2 shows a second schematic structural view of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure;

FIG. 3 shows a third schematic structural view of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure;

FIG. 4 shows a fourth schematic structural diagram of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure;

FIG. 5 shows a schematic diagram of a fifth configuration of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure;

FIG. 6 shows a sixth schematic structural view of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure;

FIG. 7 shows a seventh schematic structural diagram of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure.

The reference numbers in the figures illustrate:

1. a main body; 11. a carrier module; 111. a light through hole; 112. a first observation hole; 113. a groove; 114. mounting a plate; 115. mounting holes; 12. a heating module; 121. a heating element; 122. a temperature sensor; 123. an overheat protection module; 13. a light emitting module; 14. an observation window; 15. a cover body; 151. pressing a plate; 152. a magnetic switch; 153. a cover body mounting plate; 154. an elastic member; 155. a press plate groove; 16. a housing; 161. mounting a plate mounting post; 162. a button mounting post; 17. a control module; 18. a power supply module; 2. a reaction tube; 21. an object to be tested; 3. a darkroom housing; 31. a second observation hole; 32. a fixing mechanism; 321. a camera mounting hole; 322. a slide rail; 323. fixing the stud; 324. a slider; 325. and (7) a rubber pad.

Detailed Description

In order to make the objects, features and advantages of the present disclosure more apparent and understandable, the technical solutions in the embodiments of the present disclosure will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present disclosure, and it is apparent that the described embodiments are only a part of the embodiments of the present disclosure, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments disclosed herein without making any creative effort, shall fall within the protection scope of the present disclosure.

FIG. 1 shows a first schematic structural diagram of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure; FIG. 2 shows a second schematic structural diagram of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure; FIG. 3 shows a third schematic structural view of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure; FIG. 4 shows a fourth schematic structural diagram of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure; please refer to fig. 1, fig. 2, fig. 3 and fig. 4;

according to a first aspect of the present disclosure, there is provided a portable nucleic acid detecting apparatus including a main body 1; the main body 1 is provided with an installation cavity for installing the bearing module 11, the bearing module 11 is used for accommodating the reaction tube 2, and the reaction tube 2 is used for accommodating the object to be tested 21; the bearing module 11 is connected with a heating module 12, and the heating module 12 is used for adjusting the temperature of the bearing module 11 so as to adjust the temperature of the reaction tube 2; the main body 1 is connected with a light emitting module 13, the light emitting module 13 is arranged at the bottom of the bearing module 11, and the bearing module 11 is provided with a light through hole 111 facing the light emitting module 13, so that the emitted light of the light emitting module 13 irradiates on the reaction tube 2 through the light through hole 111; the main body 1 is further provided with an observation window 14, a first observation hole 112 is formed in the first side of the bearing module 11, and the first observation hole 112 is used for observing the reaction tube 2; the observation window 14 is used to observe the reaction tube 2 through the first observation hole 112 in a state where the carrier module 11 is mounted in the installation cavity.

The portable nucleic acid detection equipment and the system thereof place the reaction tube 2 containing the object to be detected 21 in the bearing module 11; mounting the carrier module 11 in the mounting cavity; according to a detection instruction from the mobile terminal equipment, the heating module 12 is controlled to heat the bearing module 11 to a specified temperature, and the light-emitting module 13 is controlled to emit light, so that the emitted light irradiates on the reaction tube 2 through the light-transmitting hole 111; make determinand 21 fully react in reaction tube 2 through heating reaction tube 2, emit light through light emitting module 13 to make the emission light shine on reaction tube 2 through light passing hole 111, thereby observe the fluorescence reaction of reaction tube 2 under the shining of emission light and confirm the testing result, this application step is simple, the flow is convenient, can be quick obtain determinand 21's examination result, moreover equipment simple structure, light, can conveniently carry and the family uses.

Further, the reaction tube 2 may be observed directly through the observation window 14 without using the light emitting module 13, and the reaction tube 2 may be subjected to chemiluminescence, colorimetric reaction, or the like. Thereby improve the flexible application of nucleic acid check out test set, can be adapted to multiple detection mode, more accord with domestic or portable demand.

In the embodiment of the present disclosure, the main body 1 refers to a main component of the apparatus, specifically, the main body 1 includes a housing 16 and a cover 15, the housing 16 is used for mounting the bearing module 11, the cover 15 is used for covering the housing 16, and when the cover 15 covers the housing 16, a mounting cavity for mounting the bearing module 11 is formed; a plurality of grooves 113 are formed in the bearing module 11, and the grooves 113 are used for accommodating the reaction tubes 2, specifically, the reaction tubes 2 are inserted into the grooves. Wherein, reaction tube 2 is used for supplying determinand 21 holding, and is concrete, and reaction tube 2 still includes and tests used test reagent to determinand 21, mixes through test reagent and determinand 21, carries out the detection reaction in reaction tube 2 to realize the detection to determinand 21. Wherein, the reaction tube 2 needs to be heated in the detection process, the bearing module 11 is connected with a heating module 12, and the heating module 12 is used for adjusting the temperature of the bearing module 11 so as to adjust the temperature of the reaction tube 2; the heating module 12 is connected to a second side of the carrier module 11, the second side being different from the first side; specifically, the second side and the first side are opposite in direction, and the heating module 12 includes a heating element 121, a temperature sensor 122 and an overheating protection module 123; the temperature sensor 122 is disposed between the heating element 121 and the carrying module 11, the heating module 12 is used for adjusting the temperature of the carrying module 11, and the temperature sensor 122 is used for detecting the temperature of the carrying module 11; the temperature sensor 122 is connected with an overheating protection module 123, and the overheating protection module 123 is connected with the heating element 121; the overheat protection module 123 is configured to stop heating of the heating element 121 when the temperature sensor 122 detects that the obtained temperature is higher than a predetermined temperature. The designated temperature may be preset by a worker according to the property of the object 21. Specifically, the bearing module 11 and the heating module 12 may be provided with mounting holes 115, and the mounting holes 115 are located at two ends to cooperate with fasteners to fix the heating module 12 on the bearing module 11. The temperature sensor 122 at least comprises one or more of a thermistor, a platinum resistor and a thermocouple, and the heating element 121 at least comprises one or more of a ceramic heating plate, an electrothermal film, a heating wire, a power resistor and a semiconductor heating plate. The equipment is provided with a control module 17, wherein the control module 17 is connected with a heating module 12 and used for controlling the heating module 12 so as to adjust the temperature of the bearing module 11; the control module 17 is further connected with a light emitting module 13 for controlling the light emitting module 13 to emit light to irradiate the reaction tube 2. Specifically, the control module 17 may include a voltage reduction module for converting 24V to 5V of the working voltage of the single chip microcomputer; the low-dropout voltage stabilizing module is used for converting 5V into 3.3V; the heating driving circuit controls heating by adopting PWM; and the temperature acquisition circuit, the crystal oscillator circuit and the restart circuit.

The main body 1 is connected with a light emitting module 13, the light emitting module 13 is arranged at the bottom of the bearing module 11, and the bearing module 11 is provided with a light through hole 111 facing the light emitting module 13, so that the emitted light of the light emitting module 13 irradiates on the reaction tube 2 through the light through hole 111; specifically, the number of the light holes 111 is the same as that of the grooves 113, the light holes 111 are disposed at the bottom of the grooves 113, and the light-emitting module 13 is fastened to the bottom of the carrying module 11, so that the light emitted from the light-emitting module 13 irradiates the reaction tubes 2 through the light holes 111; specifically, the emitted light is perpendicular to the reaction tube 2, and the emitted light is distributed perpendicular to the first observation hole 112, so that the emitted light does not directly enter the first observation hole 112, and the effect of the emitted light on the observation of the observation hole is minimized. The main body 1 is further provided with an observation window 14, a first observation hole 112 is formed in the first side of the bearing module 11, and the first observation hole 112 is used for observing the reaction tube 2; the first observation hole 112 is a trapezoidal hole, and the section of the first observation hole 112 is just tangent to the bearing cavity of the conical reaction tube 2, so that all the reaction liquid can be observed by the first observation hole 112; the observation window 14 is used to observe the reaction tube 2 through the first observation hole 112 in a state where the carrier module 11 is installed in the installation cavity. Specifically, a mounting plate 114 is connected above the carrier module 11, the mounting plate 114 is used for cooperating with the main body 1 to fix the carrier module 11 in the mounting cavity, specifically, a mounting column is formed inside the housing 16, and the mounting column is used for cooperating with the mounting plate 114 to fix the carrier module 11, so that the observation hole in the carrier module 11 is matched with the observation window 14 in the housing 16 in position. For the convenience of observation, an optical filter may be installed in the observation window 14. Further, the observation window 14 may include an observation window 14 port, a filter and a light barrier, the filter may be an orange acrylic plate with low price to reduce the cost of the instrument, the purpose of the filter is to filter the excitation light signal in the observation hole and pass through the fluorescence signal, and the purpose of the light barrier is to place the light signal at other places except the observation hole to affect the observation result. The detection result can be obtained in two ways, one is that a detection person directly observes whether the reaction tube 2 has a fluorescent signal through the observation window 14, so as to qualitatively obtain the detection result; the other method is to acquire and store images by using a mobile camera through software developed by a mobile terminal, and then obtain a detection result by processing the images. The main body 1 is further provided with a power module 18, and the power module 18 may be an internal power supply and/or an external power supply. In the case where the power module 18 includes an external power source, the housing 16 may be provided with a power socket, and further, a boat-shaped switch may be provided to cooperate with the power module 18 to control the power to be turned on or off. In addition, a button switch for instructing to perform a designated function may be further provided on the housing 16, and the button switch is connected to the control module 17 to perform the designated function, such as a start button, an end button, a warm-up button, and the like, to be used in cooperation with a user.

Specifically, the method for controlling the portable nucleic acid detecting device based on the button switch is provided for the button switch: under the condition of first use, control instructions corresponding to the control buttons are stored in the control module 17, and the control instructions related to the control buttons can be triggered by clicking the button switches; specifically, for example, the start button may store a first operation parameter, and the first operation parameter may control the nucleic acid detecting apparatus to detect the reaction tube 2 according to the first operation parameter. The first operating parameter is pre-stored by a worker. Further, after the mobile terminal device performs a complete nucleic acid detection on the nucleic acid detection device, the control module 17 may store the current detection parameter as the second operation parameter, and then, if the operation of the nucleic acid detection is triggered by the button switch, read the second operation parameter in the memory, and instruct the portable nucleic acid detection device to perform the nucleic acid detection by using the second operation parameter. Specifically, the first operating parameter and the second operating parameter may include: time parameters, temperature parameters, standing parameters, and the like. Therefore, the equipment can carry out detection work under the working environment with or without a mobile network so as to adapt to the working environment mostly, thereby improving the portability.

FIG. 5 shows a schematic structural diagram of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure; FIG. 6 shows a sixth schematic structural view of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure; please refer to fig. 5 and 6;

in an embodiment, the main body 1 comprises a cover 15 and a housing 16, the cover 15 and the housing 16 are rotatably connected, and the housing 16 is used for abutting the bearing module 11; the cover 15 includes a pressing plate 151, the pressing plate 151 is elastically connected to the cover 15 by an elastic member 154, and the pressing plate 151 is used to press the reaction tube 2 in the carrier module 11 when the cover 15 is closed on the housing 16.

In the embodiment of the present disclosure, the main body 1 includes a cover 15 and a housing 16, and specifically, a hinge connection may be adopted; the lid 15 includes clamp plate 151, and clamp plate 151 is the C font, back and lid 15 butt, C font both ends with bear the weight of the laminating of module 11, go on the heat conduction of heating module 12 through C font both ends like this, can prevent like this that the reaction in-process, the upper cover produces the condensation. The pressing plate 151 is elastically connected to the cover 15 by an elastic member 154, and specifically, the elastic member 154 may be a spring connected to the cover by a guide shaft. When the cover 15 is closed on the housing 16, both ends of the C-shape of the pressing plate 151 abut on the carrier module 11 to press the reaction tube 2. The cover 15 is provided with a magnetic switch 152, and the magnetic switch 152 is used to cooperate with the housing 16 to open or close the cover 15. The magnetic switch 152 may be two magnetic pieces, or one magnetic piece is matched with a metal piece. Specifically, the cover 15 may be provided with a cover mounting plate 153, and the cover mounting plate 153 is formed with a pressing plate groove 155 to which the pressing plate 151 is connected. Specifically, the housing 16 may further include a mounting plate mounting post 161 for fixing with the mounting plate 114 of the carrier module 11; also included is a button mounting post 162 for mating with the mounting and securing of the button.

FIG. 7 shows a seventh schematic structural diagram of a portable nucleic acid detecting apparatus according to an embodiment of the present disclosure; please refer to fig. 7;

in an implementation mode, the equipment further comprises a darkroom shell 3, and the darkroom shell 3 can be used for installing mobile terminal equipment or a camera; the darkroom shell 3 is provided with a second observation hole 31, and the second observation hole 31 is used for being matched with a mobile terminal device or a camera to observe the reaction tube 2. The darkroom casing 3 is provided with a fixing mechanism 32, and the fixing mechanism 32 is used for fixing the mobile terminal device so that the mobile terminal device can observe the reaction tube 2 through the second observation hole 31.

In this disclosed embodiment, it needs to have relative dark ring border to shoot, avoids the light filter reflection of light to influence the effect of shooing, leads to influencing the testing result, if will accomplish real-time quantitative detection, needs to remove end equipment or camera and shoot and handle in real time, so with the packing carton design of instrument for being fit for the structure as removing end equipment or camera darkroom of shooing. Therefore, the darkroom shell 3 is provided with a darkroom for loading a camera or moving end equipment to take pictures, the darkroom shell 3 is connected with a bottom plate, the bottom plate is provided with an installation groove for positioning and installing the portable nucleic acid detection equipment, and an installation step matched with the darkroom shell 3 is also arranged on the bottom plate; a fixing mechanism 32 for fixing the mobile terminal device may be connected to a surface of the housing 16 in which the second observation hole 31 is provided; the fixing mechanism 32 includes a first mechanism for mounting the camera, specifically, a camera mounting hole 321. Or a second mechanism for installing the mobile terminal device, specifically, the second mechanism includes a sliding rail 322, a fixing stud 323, a sliding block 324 and a rubber pad 325, the sliding block is disposed inside the sliding rail and can move along the sliding rail, one end of the sliding block is connected with the fixing stud, and the fixing stud is used for fastening the sliding block on the sliding rail, so that the sliding block fastens the mobile terminal device. One side of the sliding block towards the mobile terminal equipment is provided with a rubber pad, and the rubber pad is used for preventing the mobile terminal equipment from being scratched. Remove end equipment and nucleic acid detection equipment communication connection, it is specific, can be to be connected with control module 17 to the realization specifically includes: adjusting heating time and heating temperature; and acquiring real-time temperature information, reaction time information and detection results. Specifically, the control module is connected with a mobile terminal device and used for receiving a control instruction from the mobile terminal device so as to control the heating module to adjust the temperature of the bearing module through the control instruction, or control the light-emitting module to emit light through the control instruction.

A specific embodiment is provided: firstly, adding an object to be detected 21 (specifically, a detection sample) and a matched reagent into a test tube, wherein the detection amount at one time can be eight channels at most, placing the added sample test tube into a bearing module 11, covering a cover body 15, wherein the cover body 15 is connected with one end of a shell 16 through a hinge, and the other end of the cover body 15 is opened and closed through a magnetic switch 152; after the cover body 15 is covered, the pressure plate 151 in the structure of the cover body 15 receives the compression elastic force of the spring, the test tube is compressed by the force, the leakage caused by forced opening of the test tube port due to heating expansion of gas in the test tube in the reaction is avoided, the pressure plate 151 is tightly attached to the sample bearing module 11 at the same time, the heat of the sample bearing module 11 can be conducted to the pressure plate 151, and a better temperature control effect can be achieved; the control module 17 is divided into two parts, one is a heating module 12, and the other is a light-emitting module 13; the heating module 12 is controlled by a heating button switch, and the heating module 12 executes heating according to the temperature and time flow set by the reaction; the light-emitting module 13 is controlled to be started and stopped by an exciting light button switch, so that the reaction condition can be observed conveniently at any time; after the reaction is finished, the light-emitting module 13 is turned on, and the result can be observed by naked eyes, automatically recognized by photographing by mobile terminal equipment, and recognized by an integrated camera (such as an open mv camera); the mobile terminal equipment is wirelessly connected with the equipment, and can also control heating and excitation light switches; and heating parameters such as heating time, heating temperature and the like of a heating program can be modified so as to be suitable for isothermal amplification reactions at different temperatures.

Specifically, two specific application scenarios are provided;

application scenario 1, alcohol tolerance detection:

the alcohol tolerance of the human body is divided into three grades, namely homozygous wild type-alcohol tolerance, heterozygous mutant type-alcohol slight tolerance and homozygous mutant type-alcohol intolerance; when detecting alcohol tolerance, two different reaction systems are required to be configured for a sample, primers are respectively designed for wild type and mutant type, and if only the wild type primer reaction system is amplified, the sample is homozygous wild type; if only the mutant primer reaction system generates amplification, the mutant is indicated as a homozygous mutant; if both reaction systems produce amplification, a heterozygous mutant is indicated.

The alcohol tolerance detection method comprises the following steps: 1. and inputting information of the person to be detected. 2. Sampling, and wiping the epithelial cells of the oral cavity by using a sampling swab to finish sampling. 3. And (3) preparing and preheating the reaction crude extract, immersing the adopted detection sample swab into a nucleic acid release (cracking) reagent, and standing for 3-5 minutes to obtain the nucleic acid crude extract. 4. Simultaneously, the power supply of the detector is inserted and the power switch is turned on to preheat the instrument. 5. Reaction configuration and loading, namely dripping the crude nucleic acid extracting solution into a reaction tube 2 containing a nucleic acid amplification freeze-drying reagent, wherein the freeze-drying reagent comprises a primer, an enzyme and other reactants, shaking to completely dissolve and mix the freeze-drying reagent, and loading the reaction tube 2 loaded with the reagent into a sample bearing module 11. 6. The reaction is carried out, the preset temperature is 63 ℃, and the preset time is 30 minutes; pressing the heating key of the detector again to finish isothermal nucleic acid amplification according to the preset temperature and the preset time. 7. Detecting the result, turning on an exciting light switch, directly observing the result, and if only the wild type primer reaction system generates a fluorescent signal, indicating that the wild type primer reaction system is homozygous; if only the mutant primer reaction system generates a fluorescent signal, the mutant is a homozygous mutant; if both reaction systems produce fluorescent signals, the hybrid mutant type is indicated; the result can be acquired by adopting a mobile end camera or an integrated camera; of course, real-time quantitative amplification detection can also be realized.

Application scenario 2, SARS-Cov-2 detection: SARS-CoV-2 is an enveloped positive-strand RNA virus belonging to the genus beta coronavirus, causing a 2019 global pandemic of coronavirus disease (COVID-19). The high-efficiency and quick detection of the virus has important significance for early discovery and prevention of virus transmission. This example designed amplification primers for SARS-CoV-2ORF1ab and N gene conserved sequence, respectively, for rapid screening of SARS-CoV-2 and its mutant strains.

The SARS-Cov-2 detection comprises the following steps: 1. and inputting information of the person to be detected. 2. Sampling, namely sampling a nasal/pharyngeal swab by using a sampling swab to finish sampling. 3. And preparing a reaction crude extract. Immersing a sample swab to be detected into nucleic acid for releasing (cracking and reagent, standing for 3-5 minutes to obtain a crude nucleic acid extracting solution, preheating, preparing a crude reaction extracting solution, simultaneously inserting a power supply of a detector, turning on a power supply switch, preheating an instrument, 5, preparing and loading a reaction, dripping the crude nucleic acid extracting solution into a reaction tube 2 containing a freeze-dried nucleic acid amplification reagent, shaking to completely dissolve and mix the freeze-dried reagent, loading the reaction tube 2 loaded with the reagent into a sample bearing module 11.6, carrying out the reaction at a preset temperature of 63 ℃ for 30 minutes, pressing a heating key of the detector again, completing nucleic acid amplification according to a preset temperature and a preset time, 7, detecting the result, turning on an exciting light switch, directly observing the result, if a fluorescence signal is generated by a reaction system, determining that the result is SARS-Cov-2 positive, and vice versa, and obtaining the result, and acquiring the result by adopting a movable end camera or an integrated camera.

According to a second aspect of the present disclosure, there is provided a portable nucleic acid detection system, the system comprising: a mobile terminal device and any one of the portable nucleic acid detecting devices; the mobile terminal device is in communication connection with the portable nucleic acid detection device so as to cooperate with the portable nucleic acid detection device to perform detection operation.

According to a third aspect of the present disclosure, there is provided a nucleic acid detection method comprising: placing the reaction tube 2 containing the object to be tested 21 in the bearing module 11; mounting the carrier module 11 in the mounting cavity; according to a detection instruction from the mobile terminal equipment, controlling the heating module 12 to heat the bearing module 11 to a specified temperature so as to enable the reaction tube 2 to be at the specified temperature; according to a detection instruction from the mobile terminal device, the light emitting module 13 is controlled to emit light, so that the emitted light irradiates on the reaction tube 2 through the light through hole 111; the observation window 14 on the main body 1 is matched with the first observation hole 112 on the bearing module 11 to observe the reaction tube 2, and the detection result is determined according to the fluorescent reaction of the reaction tube 2 under the irradiation of the emitted light. In addition, the nucleic acid detection can also be realized by adopting chemiluminescence, colorimetric reaction and the like without using a light-emitting module, so the method can be suitable for various use conditions, and the application range of nucleic acid detection equipment is greatly improved.

The mobile terminal device is in communication connection with the nucleic acid detection device, and specifically, the mobile terminal device can be connected with a control module 17;

the mobile terminal device sends a control instruction to the nucleic acid detecting device, or receives feedback information from the nucleic acid detecting device, and specifically, the control instruction may include: adjusting heating time and heating temperature; the obtaining of the feedback information may include: real-time temperature information, reaction time information and detection results are provided for a user to observe at any time, so that a non-contact detection mode is realized, and the risk of infection possibly occurring in the detection process is reduced.

A specific embodiment is provided: the invention provides a nucleic acid amplification detection analysis method, which comprises the following steps:

s1, inputting information of a person to be detected, specifically, the information can be manually input by a mobile terminal equipment program, and the information can also be input by scanning a health code for the mobile terminal equipment program.

And S2, sampling the nucleic acid, namely sampling by adopting a nucleic acid sampling swab.

And S3, preparing a reaction crude extract, namely immersing the adopted detection sample swab into nucleic acid for releasing (cracking and reagent to obtain the nucleic acid crude extract.

And S4, simultaneously inserting a detector power supply and turning on a power switch to preheat the instrument.

S5, reaction configuration and loading; specifically, the crude nucleic acid extract is dropped into the reaction tube 2 containing the nucleic acid amplification lyophilized reagent, the lyophilized reagent is completely dissolved and mixed by shaking, and the reaction tube 2 loaded with the reagent is loaded into the sample loading module 11.

S6, carrying out detection reaction; specifically, a functional button for heating the detector is pressed to finish rapid isothermal nucleic acid amplification according to a preset temperature and a preset time.

S7, obtaining result detection, specifically, three result detection methods can be selected for the result detection of the portable nucleic acid detection device, namely any one of the following A, B or C.

A: the detection personnel directly observe whether the reaction tube 2 has a fluorescent signal through the observation window so as to qualitatively obtain the detection result.

B: through software developed by mobile terminal equipment, a camera of the mobile terminal equipment is used for acquiring and storing images, and then a detection result is obtained by processing the images; the method can realize the end point detection, and can also draw a real-time amplification curve on a screen of mobile terminal equipment after finishing real-time sampling according to a set program and a time interval to finish the real-time detection.

C: the method can realize end point detection, and also can transmit data to mobile terminal equipment through a wireless module to draw a real-time amplification curve after completing real-time sampling according to a set program and time intervals, so as to complete real-time detection.

S8, storing and sharing results, and respectively inputting the results in two corresponding modes, namely any one of the following D or E, according to the two detection methods;

d: aiming at the mode that the detection personnel directly observe the reaction tube 2 through the observation window to qualitatively obtain the detection results, the detection results can be in one-to-one correspondence with the information of the participants through a manual filling mode; and stores the detection information.

E: the specific reaction tubes 2 can be in one-to-one correspondence with the information of the participants through the mode of obtaining the detection result through image processing, and the detection information is stored.

And the mobile terminal equipment software can automatically store the geographic position of the current detection point while storing the result, associate the detection result with the geographic position in the map, and click the corresponding detection point position on the map to check the corresponding detection result.

It should be understood that various forms of the flows shown above, reordering, adding or deleting steps, may be used. For example, the steps described in the present disclosure may be executed in parallel, sequentially, or in different orders, as long as the desired results of the technical solutions disclosed in the present disclosure can be achieved, and the present disclosure is not limited herein.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present disclosure, "a plurality" means two or more unless specifically limited otherwise.

The above is only a specific embodiment of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of changes or substitutions within the technical scope of the present disclosure, and shall be covered by the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.

Claims (10)

1. A portable nucleic acid detecting apparatus, characterized in that the apparatus comprises a main body (1);

the main body (1) is provided with an installation cavity for installing a bearing module (11), the bearing module (11) is used for accommodating the reaction tube (2), and the reaction tube (2) is used for accommodating an object to be detected (21);

the carrying module (11) is connected with a heating module (12), and the heating module (12) is used for adjusting the temperature of the carrying module (11) so as to adjust the temperature of the reaction tube (2);

the main body (1) is connected with a light-emitting module (13), the light-emitting module (13) is arranged at the bottom of the bearing module (11), and the bearing module (11) is provided with a light through hole (111) facing the light-emitting module (13), so that the emitted light of the light-emitting module (13) is irradiated on the reaction tube (2) through the light through hole (111);

the main body (1) is also provided with an observation window (14), a first observation hole (112) is formed in the first side of the bearing module (11), and the first observation hole (112) is used for observing the reaction tube (2); the observation window (14) is used for observing the reaction tube (2) through the first observation hole (112) when the bearing module (11) is installed in the installation cavity.

2. The portable nucleic acid detecting apparatus according to claim 1, wherein the carrying module (11) is provided with a plurality of grooves (113), and the grooves (113) are used for embedding the reaction tubes (2);

the number of the light through holes (111) is consistent with that of the grooves (113), and the light emitting module (13) is fixedly connected to the bottom of the bearing module (11);

the bearing module (11) further comprises a mounting plate (114), and the mounting plate (114) is used for being matched with the main body (1) so as to fix the bearing module (11) in the mounting cavity.

3. The portable nucleic acid detecting apparatus according to claim 1,

the heating module (12) is connected to a second side of the carrier module (11), the second side being different from the first side;

the heating module (12) comprises a heating element (121), a temperature sensor (122) and an overheating protection module (123), the temperature sensor (122) being arranged between the heating element (121) and the carrier module (11), the temperature sensor (122) being used to detect the temperature of the carrier module (11);

an overheating protection module (123) is connected to the temperature sensor (122), and the overheating protection module (123) is connected with the heating element (121); the overheating protection module (123) is used for stopping heating of the heating element (121) when the temperature detected by the temperature sensor (122) is higher than a specified temperature.

4. The portable nucleic acid detecting apparatus according to claim 3,

the temperature sensor (122) at least comprises one of a thermistor, a platinum resistor and a thermocouple, and the heating element (121) at least comprises one of a ceramic heating sheet, an electrothermal film, a heating wire, a power resistor and a semiconductor heating sheet.

5. The portable nucleic acid detecting apparatus according to claim 1,

the main body (1) comprises a cover body (15) and a shell body (16), the cover body (15) is rotatably connected with the shell body (16), and the shell body (16) is used for abutting a bearing module (11); the cover body (15) comprises a pressing plate (151), the pressing plate (151) is elastically connected to the cover body (15) through an elastic piece (154), and under the condition that the cover body (15) is covered on the shell (16), the pressing plate (151) is used for pressing the reaction tube (2) in the bearing module (11).

6. The portable nucleic acid detecting device according to claim 5, wherein a magnetic switch (152) is disposed on the cover (15), and the magnetic switch (152) is used to engage with the housing (16) to open or close the cover (15).

7. The portable nucleic acid detecting apparatus according to claim 1, wherein the apparatus is provided with a control module (17), the control module (17) being connected with the heating module (12) for controlling the heating module (12) to adjust the temperature of the carrying module (11);

the control module (17) is also connected with the light-emitting module (13) and is used for controlling the light-emitting module (13) to emit light so as to irradiate the reaction tube (2);

the control module (17) is connected with a mobile terminal device and is used for receiving a control instruction from the mobile terminal device so as to control the heating module (12) to adjust the temperature of the bearing module (11) through the control instruction; or, the light-emitting module (13) is controlled to emit light through the control instruction.

8. The portable nucleic acid detecting apparatus according to claim 1, wherein the apparatus further comprises a camera housing (3), and the camera housing (3) is adapted to be mounted to a mobile terminal apparatus or a camera;

and a second observation hole (31) is formed in the darkroom shell (3), and the second observation hole (31) is used for being matched with the mobile terminal device or the camera to observe the reaction tube (2).

9. The portable nucleic acid detecting apparatus according to claim 8, wherein a fixing mechanism (32) is provided on the dark room casing (3), and the fixing mechanism (32) is used for fixing the mobile terminal apparatus so that the mobile terminal apparatus observes the reaction tube (2) through the second observing hole (31).

10. A system, characterized in that the system comprises: a mobile terminal device and the portable nucleic acid detecting device according to any one of claims 1 to 9;

the mobile terminal device is in communication connection with the portable nucleic acid detection device so as to cooperate with the portable nucleic acid detection device to perform detection operation.

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