CN217651227U

CN217651227U - Fluorescent quantitative PCR instrument

Info

Publication number: CN217651227U
Application number: CN202221255193.1U
Authority: CN
Inventors: 杨小力
Original assignee: Hebei Donghan Medical Device Technology Co ltd
Current assignee: Hebei Donghan Medical Device Technology Co ltd
Priority date: 2022-05-23
Filing date: 2022-05-23
Publication date: 2022-10-25
Anticipated expiration: 2032-05-23

Abstract

The utility model discloses a fluorescence quantitative PCR appearance, which comprises a body, the nearly lower part of inner chamber of body is equipped with heated warehouses, heated warehouses's bottom is provided with the heating piece, heated warehouses's upper portion one side is provided with the screw rod, screw-thread fit is provided with first supporting block on the screw rod, the side of first supporting block is provided with a supporting bench, a supporting bench's upper portion combination is provided with the test tube pallet, another terminal surface of supporting bench is provided with the second supporting shoe, the terminal surface of second supporting shoe runs through to slide and is provided with vertical guide arm, a side end fixing is provided with power pump sending subassembly in the heated warehouses, the top of body is provided with the bulge loop, the upper end of bulge loop agrees with and is provided with the lid, nearly top is provided with scattered hot-blast organism in the lid. The utility model discloses a this internal water bath heating, and the temperature adjusts in real time, and the test tube that is equipped with detection liquid is heated evenly, in addition, reduces test tube temperature fast, has improved detection efficiency.

Description

Fluorescent quantitative PCR instrument

Technical Field

The utility model relates to a biological detection correlation equipment technical field specifically is a fluorescence quantitative PCR appearance.

Background

The fluorescence quantitative PCR instrument heats a PCR reaction system added with a fluorescent group for a certain time at a certain temperature, so that the reaction system finishes thermal cycle of high-temperature denaturation, low-temperature renaturation and proper-temperature extension, fluorescein is dissociated in the reaction system, then the PCR reaction change is detected in real time by utilizing the change of a fluorescence signal, and the quantitative analysis of an initial template is realized through the relation between a Ct value and a standard curve.

However, current fluorescence quantitative PCR appearance can be higher because the reaction temperature who sets for in the heating stage, and the back that leads to the test tube heating to finish, and the bulk temperature of test tube is higher, and the experimenter can't carry out follow-up quantitative analysis operation immediately, and then needs a large amount of wait cooling time, has effectively reduced experimenter's work efficiency.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a fluorescence quantitative PCR appearance to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a fluorescence quantitative PCR appearance, includes the body, the nearly lower part of inner chamber of body is equipped with the heated warehouses, the bottom of heated warehouses is provided with the heating piece, upper portion one side of heated warehouses is provided with the screw rod, screw-thread fit is provided with first supporting block on the screw rod, the side of first supporting block is provided with a supporting bench, the terminal surface of supporting bench runs through and is equipped with a plurality of jacks, the combination of supporting bench upper portion is provided with the test tube pallet, another terminal surface of supporting bench is provided with the second supporting shoe, the terminal surface of second supporting shoe runs through to slide and is provided with vertical guide arm, the upside of heated warehouses and be provided with the ventilation structure board at the both sides tip fluting of body, a side end fixing is provided with power pumping subassembly in the heated warehouses, the top of body is provided with the bulge loop, the upper end of bulge loop agrees with and is provided with the lid, the top is provided with the ventilation net in the lid, the lower part contact of ventilation net is provided with scattered hot-air organism.

Preferably, a plurality of test tubes that test tube stand terminal surface was equipped with are identical with a plurality of jacks.

Preferably, a power motor is arranged at the upper end of the screw rod, and the output end of the power motor is connected with the screw rod through a coupler.

Preferably, the embedding of heating chamber inside portion is provided with temperature detection sensor, temperature detection sensor pass through temperature controller with heating block electric connection.

Preferably, the power pumping component comprises a pump body, a first conveying pipe and a second conveying pipe are respectively arranged at two side ends of the pump body, the first conveying pipe is designed towards the heating bin, a three-way joint is arranged at the tail end of the first conveying pipe, two conveying pipes are inserted and sleeved at ports on the other two sides of the three-way joint, a three-way joint and two conveying pipes are also arranged at the outer end of the second conveying pipe, an electromagnetic valve is arranged on each conveying pipe, and a water storage bin is arranged at the tail end of the second conveying pipe.

Preferably, the lower end of the ventilation structure plate is provided with a limiting block, and a limiting groove is formed in the outer portion of the limiting block and in the groove formed in the end portion of the body.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model discloses heat block in the heated warehouses that sets up, temperature detection sensor, power pump sending subassembly cooperatees and uses, under the pump body effect, and open one of them raceway of end-to-end connection on the first defeated pipe and the solenoid valve of one of them raceway of end-to-end connection on the second defeated pipe, make in the heated warehouses continue to be full of a certain amount of water, then launch the heat block, carry out water bath heating to the test tube, effectively kept the test tube heated evenly, afterwards, after the heating is accomplished, can be under power pump sending subassembly again, with in the heated warehouses hot water pump send to the reservoir, make the test tube break away from the heating system in the heated warehouses, then the power motor who connects on the screw rod, drive the test tube pallet and shift up, and under the effect of cooling fan body, let in the body inner chamber with outside air current via the ventilation structure board, later by the output of ventilation net, air flow rate has been accelerated, the temperature of test tube has been reduced fast, do benefit to carrying out the PCR appearance operation on next step;

2. the utility model discloses the test tube pallet and the brace table that still set up simultaneously under the motor power effect, shift up to bulge loop department with the brace table, can directly peg graft at the test tube pallet with batch test tube to the fixed test tube pallet who makes up there is the test tube of a test tube through the jack of brace table terminal surface, the operation of batch nature has further improved experimenter's work detection efficiency.

Drawings

FIG. 1 is a front sectional view of the structure of an embodiment of the present invention;

FIG. 2 is an enlarged view of the structure A of FIG. 1;

FIG. 3 is a view showing the overall appearance separation structure of the present invention;

FIG. 4 is a schematic view of a second embodiment of the present invention;

fig. 5 is an enlarged view of the structure B in fig. 4 according to the present invention.

In the figure: 1. a body; 2. a heating chamber; 3. a heating block; 4. a screw; 5. a first support block; 6. a support table; 7. a jack; 8. a test tube stand; 9. a second support block; 10. a vertical guide rod; 11. a ventilation structure plate; 12. a powered pumping assembly; 121. a pump body; 122. a first delivery pipe; 123. a second delivery pipe; 124. a three-way joint; 125. a water delivery pipe; 126. an electromagnetic valve; 13. a convex ring; 14. a cover body; 15. a ventilation net; 16. a heat radiating fan body; 17. a power motor; 18. a temperature detection sensor; 19. a water storage bin; 20. a limiting block; 21. a limiting groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example one

Referring to fig. 1-3, the present invention provides a technical solution: the utility model provides a fluorescence quantitative PCR appearance, which comprises a body 1, the nearly lower part of inner chamber of body 1 is equipped with heated warehouses 2, heated block 3 is installed to heated warehouses 2's bottom, screw rod 4 is installed to heated warehouses 2's upper portion one side, screw-thread fit installs first supporting block 5 on the screw rod 4, supporting bench 6 is installed to the side welding of first supporting block 5, supporting bench 6's terminal surface runs through and is equipped with a plurality of jacks 7, supporting bench 6's upper portion grafting composite mounting has test tube rack platform 8, another terminal surface welding of supporting bench 6 installs second supporting block 9, the terminal surface of second supporting block 9 runs through slidable mounting has vertical guide arm 10, heated warehouses 2's upside and install ventilation structure board 11 at body 1's both sides tip fluting, a side tip screw fixed mounting has power pumping subassembly 12 in heated warehouses 2, bulge loop 13 is installed in body 1's the top welding, lid 14 is agreed with in the upper end of bulge loop 13, top fluting fixed mounting has ventilation net 15 in the lid 14, ventilation net 15's lower part contact is installed and is scattered hot-air organism 16.

A plurality of test tubes arranged on the end surface of the test tube stand 8 are matched with the jacks 7; the upper end of the screw rod 4 is provided with a power motor 17, and the output end of the power motor 17 is connected with the screw rod 4 through a coupler; a temperature detection sensor 18 is embedded in the inner side part of the heating bin 2, and the temperature detection sensor 18 is electrically connected with the heating block 3 through a temperature controller; the power pumping component 12 comprises a pump body 121, a first conveying pipe 122 and a second conveying pipe 123 are respectively installed at two side ends of the pump body 121, the first conveying pipe 122 is designed to face the heating chamber 2, a three-way joint 124 is installed at the tail end of the first conveying pipe 122, two conveying pipes 125 are inserted and sleeved at ports on two sides of the three-way joint 124, the three-way joint 124 and the two conveying pipes 125 are also installed at the outer end of the second conveying pipe 123, an electromagnetic valve 126 is installed on each conveying pipe 125, and the water storage chamber 19 is installed at the tail end of the second conveying pipe 123.

The working principle is as follows: the utility model discloses when using, open lid 14, and under power motor 17's effect, screw rod 4's rotation can drive its first supporting shoe 5 of screw-thread fit, the overall structure that brace table 6 and second supporting shoe 9 are constituteed shifts up on vertical guide arm 10, bulge loop 13 department at body 1 top, test tube pallet 8 combination that will place the test tube through a plurality of jacks 7 that brace table 6 terminal surface runs through is at brace table 6 terminal surface, reverse screw rod 4's power motor 17 after that, make the brace table 6 that the combination has test tube pallet 8 move down to in the heating chamber 2, then the switch-on pump body 121, and open on first defeated pipe 122 on the three way connection of end-to-end connection 124 in the raceway 125 that two port grafting covers were established and the second defeated pipe 123 go up the three way connection 124 of end-to-end connection in the raceway 125 that end-to-end connection was established, make heating chamber 2 and storage chamber 19 form a water circulation route between, carry the interior water of storage chamber 19 to heating chamber 2 in, launch heating block 3 at last, and detect a plurality of port grafting cover raceway 125 on the test tube pallet 8 through the temperature detection sensor, the simple temperature adjustment effect of being heated in order to have kept the test tube to be heated water bath advantage.

Example two

Referring to fig. 3-5, the present invention provides a technical solution: the utility model provides a fluorescence quantitative PCR appearance, which comprises a body 1, the nearly lower part of inner chamber of body 1 is equipped with heated warehouses 2, heated block 3 is installed to heated warehouses 2's bottom, screw rod 4 is installed to heated warehouses 2's upper portion one side, screw-thread fit installs first supporting block 5 on the screw rod 4, supporting bench 6 is installed to the side welding of first supporting block 5, supporting bench 6's terminal surface runs through and is equipped with a plurality of jacks 7, supporting bench 6's upper portion grafting composite mounting has test tube rack platform 8, another terminal surface welding of supporting bench 6 installs second supporting block 9, the terminal surface of second supporting block 9 runs through slidable mounting has vertical guide arm 10, heated warehouses 2's upside and install ventilation structure board 11 at body 1's both sides tip fluting, a side tip screw fixed mounting has power pumping subassembly 12 in heated warehouses 2, bulge loop 13 is installed in body 1's the top welding, lid 14 is agreed with in the upper end of bulge loop 13, top fluting fixed mounting has ventilation net 15 in the lid 14, ventilation net 15's lower part contact is installed and is scattered hot-air organism 16.

A plurality of test tubes arranged on the end surface of the test tube stand 8 are matched with the jacks 7; the upper end of the screw rod 4 is provided with a power motor 17, and the output end of the power motor 17 is connected with the screw rod 4 through a coupler; a temperature detection sensor 18 is embedded in the inner side part of the heating bin 2, and the temperature detection sensor 18 is electrically connected with the heating block 3 through a temperature controller; the power pumping component 12 comprises a pump body 121, a first conveying pipe 122 and a second conveying pipe 123 are respectively installed at two side ends of the pump body 121, the first conveying pipe 122 is designed to face the heating bin 2, a three-way joint 124 is installed at the tail end of the first conveying pipe 122, two water conveying pipes 125 are inserted and sleeved at the other two side ports of the three-way joint 124, a three-way joint 124 and two water conveying pipes 125 are also installed at the outer end of the second conveying pipe 123, an electromagnetic valve 126 is installed on each water conveying pipe 125, and a water storage bin 19 is installed at the tail end of the second conveying pipe 123; the lower end of the ventilation structure plate 11 is provided with a limiting block 20, and a limiting groove 21 is arranged outside the limiting block 20 and in the groove formed in the end of the body 1.

The working principle is as follows: the utility model discloses a when using, the difference with embodiment one in this embodiment lies in the spacing groove 21 that the inslot bottom that 1 both sides tip of body was seted up was equipped with, through spacing groove 21 and the 11 lower extreme welded stopper 20 of ventilation structure board, is convenient for ventilate 11 movable dismouting of structure board in spacing groove 21 department, and convenient follow-up dust is clean.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a fluorescence quantitative PCR appearance, includes body (1), its characterized in that: the nearly lower part of inner chamber of body (1) is equipped with heating chamber (2), the bottom of heating chamber (2) is provided with heating block (3), upper portion one side of heating chamber (2) is provided with screw rod (4), screw-thread fit is provided with first supporting block (5) on screw rod (4), the side of first supporting block (5) is provided with brace table (6), the terminal surface of brace table (6) runs through and is equipped with a plurality of jacks (7), the upper portion combination of brace table (6) is provided with test tube rack platform (8), another terminal surface of brace table (6) is provided with second supporting shoe (9), the terminal surface of second supporting shoe (9) runs through the slip and is provided with vertical guide arm (10), the upside of heating chamber (2) and the both sides tip fluting of body (1) are provided with ventilation structure board (11), one side end fixing is provided with power pumping subassembly (12) in heating chamber (2), the top of body (1) is provided with bulge loop (13), the upper end of bulge loop (13) agrees with and is provided with ventilation lid (14), the top is provided with ventilation net (15) in body (15), ventilation net (16) contact.

2. The fluorescent quantitative PCR instrument according to claim 1, wherein: a plurality of test tubes that test tube stand platform (8) terminal surface was equipped with are identical with a plurality of jacks (7).

3. The fluorescent quantitative PCR instrument according to claim 1, wherein: the upper end of screw rod (4) is provided with motor power (17), just motor power's (17) output is provided with screw rod (4) through the coupling joint.

4. The fluorescent quantitative PCR instrument according to claim 1, wherein: the embedding of heating chamber (2) inside portion is provided with temperature detect sensor (18), temperature detect sensor (18) pass through temperature controller with heating block (3) electric connection.

5. The fluorescence quantitative PCR instrument according to claim 1, wherein: the power pumping assembly (12) comprises a pump body (121), a first conveying pipe (122) and a second conveying pipe (123) are arranged at two ends of the pump body (121) respectively, the first conveying pipe (122) is designed to face the heating bin (2), a three-way joint (124) is arranged at the tail end of the first conveying pipe (122), two conveying pipes (125) are sleeved at ports of the other two sides of the three-way joint (124) in an inserting mode, the outer end of the second conveying pipe (123) is also provided with the three-way joint (124) and the two conveying pipes (125), each conveying pipe (125) is provided with an electromagnetic valve (126), and the tail end of the second conveying pipe (123) is provided with a water storage bin (19).

6. The fluorescence quantitative PCR instrument according to claim 1, wherein: the lower end of the ventilation structure plate (11) is provided with a limiting block (20), and a limiting groove (21) is arranged outside the limiting block (20) and in a groove formed in the end of the body (1).