CN117143723A - PCR detection device and method - Google Patents
PCR detection device and method Download PDFInfo
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- CN117143723A CN117143723A CN202311134608.9A CN202311134608A CN117143723A CN 117143723 A CN117143723 A CN 117143723A CN 202311134608 A CN202311134608 A CN 202311134608A CN 117143723 A CN117143723 A CN 117143723A
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- pcr
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- 238000001514 detection method Methods 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims description 10
- 238000007789 sealing Methods 0.000 claims abstract description 105
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 87
- 238000010438 heat treatment Methods 0.000 claims abstract description 35
- 230000000149 penetrating effect Effects 0.000 claims abstract description 7
- 230000007246 mechanism Effects 0.000 claims description 25
- 238000001816 cooling Methods 0.000 claims description 13
- 238000005057 refrigeration Methods 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 6
- 238000005192 partition Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 230000000712 assembly Effects 0.000 claims description 3
- 238000000429 assembly Methods 0.000 claims description 3
- 230000002146 bilateral effect Effects 0.000 claims description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 47
- 230000009471 action Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 239000000498 cooling water Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000008236 heating water Substances 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the technical field of PCR detection devices, and particularly relates to a PCR detection device, which comprises a PCR detector body, a refrigerating component and a heating component are respectively arranged in two water storage tanks, a test tube rack comprises two support plates and a placing plate, the two support plates are symmetrically and fixedly connected to the lower ends of the placing plate, elastic sealing elements are fixedly connected to the lower sides of a plurality of test tube holes, empty slots are formed in the placing plate, slots are formed in the PCR detector body, a plurality of first springs are symmetrically and fixedly connected to the slots, two raised strips are fixedly connected to the upper ends of the first springs, two pull ropes are symmetrically and fixedly connected to the lower ends of the two raised strips, two sliding plates are fixedly connected with second springs, and water inlet pipes are communicated to the left sides of the two heating slots in a penetrating manner.
Description
Technical Field
The invention belongs to the technical field of PCR detection devices, and particularly relates to a PCR detection device and a PCR detection method.
Background
Polymerase Chain Reaction (PCR) is a molecular biological technique for amplifying specific DNA fragments, which can be regarded as specific DNA replication in vitro, and the greatest feature of PCR is the ability to greatly increase minute amounts of DNA. The DNA becomes single-stranded by utilizing the high temperature time variability of 95 ℃ in vitro, the primer and the single-stranded are combined according to the base complementary pairing principle at low temperature (usually about 60 ℃), the temperature is adjusted to the optimal reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes the complementary strand along the direction from phosphoric acid to pentose (5 '-3'). The PCR instrument based on polymerase is actually a temperature control device, and can well control the denaturation temperature, the renaturation temperature and the extension temperature. Then, the amplified DNA product is optically detected through a detection mechanism in the PCR detector to obtain a PCR detection result;
the existing PCR detector generally adopts a water bath heating mode to carry out thermal cycle on a detection test tube, and carries out heating or cooling treatment outside the detection test tube through solution soaking, but such a mode has the condition that temperature difference appears in the solution in the soaking process, and then causes inconsistent temperature control of the detection test tube, and simultaneously adopts the solution soaking mode to need cooling down the solution at high temperature to low temperature and then cooling down the test tube, and simultaneously needs to heat up the solution to high temperature and then heat up the test tube when the solution is at low temperature, so that the solution temperature change efficiency is low, and then influences the work efficiency of the PCR detector.
Disclosure of Invention
The purpose of the invention is that: aim at providing a PCR detection device and method, can make things convenient for operating the test tube through the structure of test tube rack directly, still can realize heating water and cooling water's circulation effect through placing the inside empty slot of board and heating groove intercommunication simultaneously, improve temperature control's speed, will place the board simultaneously and upwards lift up under the effect of first spring and elastic component after the detection is accomplished, and then conveniently take the test tube rack.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
the utility model provides a PCR detection device, includes the PCR detector body, sealed lid is installed to PCR detector body upper end, PCR detector body internally mounted has detection mechanism and temperature control mechanism, touch control screen is installed to PCR detector body front side, PCR detector body internally mounted has test tube rack, touch control screen and sealed lid, detection mechanism and temperature control mechanism electric connection, temperature control mechanism includes refrigeration subassembly and heating element, two catch basins have been seted up to PCR detector body inside, refrigeration subassembly and heating element install respectively in two catch basins inside, test tube rack includes two backup pads and places the board, two backup pad symmetry fixed connection is in place the board lower extreme, place the board and run through and set up a plurality of test tube holes, a plurality of test tube hole downside all annular fixedly connected with elastic sealing element, place the board inside set up with the empty slot of elastic sealing element intercommunication, place the board lower extreme symmetry and install the elastic element;
the PCR detector is characterized in that slots matched with the supporting plate and the elastic piece are formed in the PCR detector body, a plurality of first springs matched with the elastic piece are symmetrically and fixedly connected in the slots, two convex strips are fixedly connected to the upper ends of the first springs and are in sliding connection with the slots, two pull ropes are symmetrically and fixedly connected to the lower ends of the convex strips, two pull ropes are arranged on the left side, two sliding plates are fixedly connected to the pull ropes and are in sliding connection with the PCR detector body respectively, two sliding plates are mutually far away from one side, a second spring is fixedly connected to the two sliding plates, a water inlet pipe is communicated with the left side of the heating tank in a penetrating mode, a communicating pipe is arranged on the water inlet pipe, two water outlet pipes are respectively communicated with the right side of the heating tank in a penetrating mode, water pumps are respectively arranged on the water outlet pipes and the communicating pipe, joints are respectively arranged on the water outlet pipes and the communicating pipe, sealing holes are formed in the placing plates and are in sealing connection with the joints, and a separating mechanism is arranged in the empty tank.
The elastic piece comprises a plurality of third springs fixedly connected to the lower end of the placing plate, a plurality of trapezoid pieces fixedly connected to the same side with the third springs are connected with the supporting plate in a sliding mode, and the lower ends of the trapezoid pieces are flush with the lower end of the placing plate.
The trapezoid piece comprises two inclined plates and a transverse plate, wherein the transverse plate is in sliding connection with the placing plate, the inclined plates are symmetrically and fixedly connected with the two sides of the transverse plate, and the transverse plate is matched with the convex strips.
The separation mechanism comprises a plurality of baffles, wherein the baffles are uniformly and fixedly connected with the inside of the empty groove, the baffles and the empty groove form a plurality of independent cavities, the independent cavities are matched with the test tube holes, the sliding plates are mutually far away from one side and are respectively provided with a water diversion pipe, the two water diversion pipes are respectively communicated with the communicating pipe and the two water inlet pipes, the number and the positions of the connectors are matched with the independent cavities, and the inside of each independent cavity is provided with an automatic adjusting component.
The two mounting grooves are formed in the supporting plates, the two trapezoid pieces are fixedly connected with mounting plates in sliding connection with the mounting grooves, the automatic adjusting assembly comprises a fixed frame fixedly connected with the upper end of the mounting plates, a first magnet is fixedly connected with the inner portion of the fixed frame in a sealing sliding manner, a fourth spring is fixedly arranged between the first magnet and the mounting plates, the first magnet is in sealing sliding connection with the partition plates, the sealing holes are located above the first magnet, and starting pieces matched with the automatic adjusting assemblies are arranged at the lower ends of the sealing covers.
The starting piece comprises a butt plate fixedly connected with the lower end of a sealing cover, a plurality of sealing grooves matched with test tube holes are formed in the butt plate, sealing plates which are flush with the butt plate are connected in a sealing sliding mode in the sealing grooves, a plurality of fifth springs are fixedly connected in the sealing grooves, step grooves are formed in the butt plate in bilateral symmetry mode, two step grooves are formed in the step grooves in a sealing sliding mode, second magnets are connected in the step grooves in a sealing sliding mode, a plurality of first magnets and a plurality of second magnets are mutually close to one another, and through grooves are formed in the sealing grooves and the step grooves in a communicating mode.
The number of each transverse row of the sealing holes is 8, the elastic sealing piece is made of rubber, and the diameter size of the upper end of the elastic sealing piece is larger than that of the lower end of the elastic sealing piece.
A PCR detection method comprising the steps of:
step 1: the test tube to be detected is placed in the test tube hole on the test tube placing rack, and then the detection test tube is completely located in the test tube hole.
Step 2: the placed test tube rack is placed inside the PCR detector body, and then the raised strips are used for limiting and aligning the elastic pieces, so that the sealing cover is convenient to align the test tube rack for closing.
Step 3: after the sealing cover is aligned, sealed and closed, the connectors are in sealing connection with the sealing holes, and then the solution in the water storage tank is pumped by the water pump to enter the independent cavity for heating or cooling.
Step 4: when the temperature is required to rise, the water storage tank connected with the heating component works corresponding to the water pump of the water outlet pipe and the water pump of the communicating pipe, so that hot water heated by the circulating suction heating component enters the independent cavity to heat the detection test tube;
when cooling is needed, the water storage tank with the refrigeration component is connected with the water pump of the corresponding water outlet pipe and the water pump of the communicating pipe to work, so that the solution in the water storage tank enters the independent cavity to cool the detection test tube after the refrigeration of the refrigeration component is pumped during cooling; and the whole process continuously sucks the solution in the independent cavity to be in a circulating replacement state.
Step 5: and analyzing the PCR product after the reaction by a detection mechanism, and finally obtaining a result.
According to the invention, an operator can directly operate the test tube through the structure of the test tube placing frame, meanwhile, the empty groove in the placing plate is communicated with the heating groove, so that the circulation effect of heating water and cooling water is realized, the temperature control speed is improved, and meanwhile, the placing plate is lifted upwards under the action of the first spring and the elastic piece after the detection is finished, so that the test tube placing frame is conveniently taken.
Drawings
The invention may be further illustrated by means of non-limiting examples given in the accompanying drawings.
FIG. 1 is a schematic diagram of a PCR detection apparatus according to the present invention;
FIG. 2 is a schematic diagram showing a first cross-sectional structure of a PCR detection apparatus according to the present invention;
FIG. 3 is an enlarged schematic view of FIG. 2A;
FIG. 4 is a schematic diagram showing a second cross-sectional structure of a PCR detecting apparatus according to the present invention;
FIG. 5 is an enlarged schematic view of the structure shown at B in FIG. 4;
FIG. 6 is a schematic view showing a first cross-sectional structure of the test tube rack of the present invention;
FIG. 7 is a schematic view showing a second cross-sectional structure of the test tube rack of the present invention.
The main reference numerals are as follows:
PCR detector body 1, sealing cover 11, detection mechanism 12, touch control screen 13, test tube rack 14, refrigeration subassembly
15. Heating element 16, water reservoir 17, support plate 20, placement plate 21, elastic seal 22, empty slot 23, slot 24, first spring 26, raised strip 27, pull cord 28, slide 29, second spring 30, water inlet pipe 31, communication pipe 32, water outlet pipe 33, water pump 34, and joint 35;
third spring 40, trapezoid 41, inclined plate 42, cross plate 43, partition 44, independent chamber 45, water diversion pipe 46, mounting plate 47, fixed frame 48, first magnet 49, fourth spring 50, abutting plate 51, seal groove 52, seal plate 53, fifth spring 54, and second magnet 55.
Detailed Description
In order that those skilled in the art will better understand the present invention, the following technical scheme of the present invention will be further described with reference to the accompanying drawings and examples.
Example 1:
as shown in fig. 1-7, the PCR detection device of the present invention comprises a PCR detector body 1, a sealing cover 11 is installed at the upper end of the PCR detector body 1, a detection mechanism 12 and a temperature control mechanism are installed inside the PCR detector body 1, a touch control screen 13 is installed at the front side of the PCR detector body 1, a test tube placing rack 14 is installed inside the PCR detector body 1, the touch control screen 13 is electrically connected with the sealing cover 11, the detection mechanism 12 and the temperature control mechanism, the temperature control mechanism comprises a refrigeration component 15 and a heating component 16, two water storage tanks 17 are provided inside the PCR detector body 1, the refrigeration component 15 and the heating component 16 are respectively installed inside the two water storage tanks 17, the test tube placing rack 14 comprises two support plates 20 and a placing plate 21, the two support plates 20 are symmetrically and fixedly connected to the lower ends of the placing plate 21, the placing plate 21 is provided with a plurality of test tube holes in a penetrating way, the lower sides of the test tube holes are all fixedly connected with an elastic sealing member 22 in a ring shape, an empty slot 23 communicated with the elastic sealing member 22 is provided inside the placing plate 21, and the lower ends of the placing plate 21 are symmetrically provided with elastic members;
the PCR detector body 1 is internally provided with a slot 24 matched with the supporting plate 20 and an elastic piece, a plurality of first springs 26 matched with the elastic piece are symmetrically and fixedly connected in the slot 24, the upper ends of the plurality of first springs 26 are fixedly connected with two convex strips 27 which are in sliding connection with the slot 24, the lower ends of the two convex strips 27 are symmetrically and fixedly connected with two pull ropes 28, the left side two pull ropes 28 and the right side two pull ropes 28 are respectively and fixedly connected with a sliding plate 29 which is in sliding connection with the PCR detector body 10, one sides, far away from each other, of the two sliding plates 29 are fixedly connected with a second spring 30, the left side of the two heating grooves 27 is penetrated and communicated with a water inlet pipe 31, a communicating pipe 32 is arranged on the left side of the two heating grooves 31, the right side of the two heating grooves 27 is penetrated and communicated with a water outlet pipe 33, the two water outlet pipes 33 and the communicating pipe 32 are respectively provided with a water pump 34, the two water outlet pipes 33 and the communicating pipe 32 are respectively provided with a joint 35, the placing plate 21 is provided with a sealing hole which is in sealing connection with the joint 35, and the inside of the hollow groove 23 is provided with a separating mechanism.
In the initial state, the two raised strips 27 extend out of the slot 24 under the action of the resilience of the first spring 26, and the two sliding plates 29 are mutually far away under the action of the second spring 30, so that the connector 35 is positioned in the PCR detector body 1, the test tube rack 14 can be conveniently installed in the slot 24, the resilience of the elastic piece is matched with the two supporting plates 20 in the initial state, the test tube rack can be stably and stably placed on a table top, the test tube can be conveniently operated, meanwhile, the test tube rack 14 can be used without using an heightening auxiliary device through the arrangement of the supporting plates 20 and the thickening placing plates 21, and the test tube rack 14 is in rectangular installation through the two supporting plates 20 and the two elastic pieces, so that the test tube rack 14 is more stable in placement, and meanwhile, the test tube rack can be conveniently operated by a tester;
when in use, a test tube is inserted into a test tube hole in the test tube rack 14, then the test tube rack 14 is placed in the PCR detector body 1, the supporting plate 20 and the elastic piece are positioned in the slot 24, the test tube rack 14 is limited, then the sealing cover 11 is rotated to be in sealing connection with the upper end of the PCR detector body 1, meanwhile, the test tube rack 14 is driven to move downwards, the first spring 26 and the elastic piece are compressed, the elastic piece and the placing plate 21 completely enter the slot 24, when the sealing cover 11 drives the test tube rack 14 to compress the first spring 26, the pull rope 28 is converted into a loose state from a straightened state, the pull rope 28 is pulled by the cooperation of the second spring 30 and the sliding plate 29, the two sliding plates 29 are mutually close, the joint 33 of the two water outlet pipes 33 and the joint of the communicating pipe 32 are in sealing connection with the sealing hole, the water outlet pipe 33 and the communicating pipe 32 are telescopic water pipes and move along with the sliding plate 29, after the three joints 35 are in sealing connection with the sealing holes, the refrigerating assembly 15 and the heating assembly 16 can be started in advance when the detection function of the PCR detector body 1 is started, so that the temperature inside the two water storage tanks 17 is at the temperature required by detection, the water pump 34 is convenient to suck when the temperature of the detection test tube is controlled, when the detection test tube needs to be heated, the water pump 34 connected with the communicating pipe 32 and the water pump 34 of the water outlet pipe 33 communicated with the water storage tank 17 provided with the heating assembly 16 are started together through the touch control screen 13, thus the communicating pipe 32 can suck hot water inside the water storage tank 17 into the empty tank 23 and then be discharged from the water outlet pipe 33 communicated with the water storage tank 17, and the two water pumps 34 continuously circulate unidirectional suction, the temperature inside the air control tank 23 is kept at the temperature set by the heating component 16 continuously, when one end of the water inlet pipe 31 close to the heating component 16 needs to be cooled and closed, then the water inlet pipe 31 close to the refrigerating component 15 is communicated, the water pump 34 arranged on the communicating pipe 32 sucks cooling solution cooled by the refrigerating component 15, the cooling solution enters the communicating pipe 32 and is further conveyed into the air tank 23, the water pump 34 arranged on the water outlet pipe 33 is closed and is close to the heating side 16, the water pump 34 close to the refrigerating component 15 is started, the water outlet pipe 31, the communicating pipe 32, the air tank 23 and the water outlet pipe 33 form a circulation passage with the heating tank 27 of the refrigerating component 15, cooling water is continuously sucked into the air tank 23 to cool a detection test tube, when the detection test tube needs to be heated again, the operation is repeated, meanwhile, the other component does not stop working in the circulation heating or cooling process, the solution inside the heating tank 27 is continuously heated or cooled, and the solution at the temperature is directly sucked when the temperature is conveniently switched; after the detection is completed, the sealing cover 11 can be opened only after the solution in the empty groove 23 is pumped by the water pump 34 arranged on the water outlet pipe 31; after the sealing cover 11 is opened, the resilience force of the first spring 26 drives the elastic piece and the two supporting plates 20 to move upwards, so that the placing plate 21 leaks out to facilitate the operator to take and complete all detection operations.
The invention can facilitate the operation of an operator to the test tube directly through the structure of the test tube placing rack 14, and simultaneously can realize the circulation of heating water and cooling water through the communication between the empty groove 23 and the heating groove 27 in the placing plate 21, thereby improving the speed of temperature control, and simultaneously lifting the placing plate 21 upwards under the action of the first spring 26 and the elastic piece after the detection is finished, thereby facilitating the taking of the test tube placing rack 14.
Example 2:
further improvement on the basis of the embodiment 1, the elastic piece comprises a plurality of third springs 40 fixedly connected to the lower end of the placement plate 21, a trapezoid piece 41 which is fixedly connected with the support plate 21 on the same side together by the plurality of third springs 40, and the lower end of the trapezoid piece 41 is flush with the lower end of the placement plate 21; the resilience force of the third spring 40 in the initial state enables the trapezoid 41 to move downwards until the lower end face of the trapezoid 41 is flush with the placement plate 21, so that the trapezoid 41 and the support plate 20 are matched with each other, the placement plate 21 can be stably placed at the upper end of a tabletop, and meanwhile stability of the device can be increased due to the trapezoid arrangement.
The trapezoid piece 41 comprises two inclined plates 42 and a transverse plate 43, the transverse plate 43 is in sliding connection with the placing plate 21, the two inclined plates 42 are symmetrically and fixedly connected with two sides of the transverse plate 43, and the transverse plate 43 is matched with the raised strips 27; the transverse plate 43 is matched with the convex strips 27, so that the convex strips 27 are matched with the transverse plate 43 when the trapezoid 41 is placed in the PCR detector body 1, and the test tube placing frame 14 is limited, so that positioning is facilitated.
The separation mechanism comprises a plurality of separation plates 44, the separation plates 44 are uniformly and fixedly connected with the inside of the empty groove 23, the separation plates 44 and the empty groove 23 enclose a plurality of independent cavities 45, the independent cavities 45 are matched with test tube holes, a water diversion pipe 46 is arranged on one side, far away from each other, of the sliding plate 29, the two water diversion pipes 46 are respectively communicated with the communicating pipe 32 and the two water inlet pipes 33, the number and the positions of the joints 35 are matched with the independent cavities 45, and automatic adjusting components are arranged in the independent cavities 45;
when detecting a plurality of test tubes, the preferred horizontal row is placed, and then make the test tube that needs to detect all be located the inside convenient automatically regulated subassembly work in an independent chamber 45, and a plurality of joints 35 and independent chamber 45 assorted, and then make the solution that communicating pipe 32 got into get into a plurality of joints 35 and flow into the independent chamber 45 inside through shunt tubes 46, a plurality of independent chambers 45 keep apart like this, avoid being close to the solution that connects and change easily, and keep away from the problem that the solution that connects can't change caused the temperature difference, independent chamber 45 forms the entering and the discharge of solution more to be favorable to in this way.
The inside of the two supporting plates 20 is provided with mounting grooves, the two trapezoidal pieces 41 are fixedly connected with mounting plates 47 which are in sliding connection with the mounting grooves, the automatic adjusting assembly comprises a fixed frame 48 which is fixedly connected with the upper ends of the mounting plates 47, the inside of the fixed frame 48 is in sealing sliding connection with a first magnet 49, the first magnet 49 and the mounting plates 47 are fixedly provided with a fourth spring 50, the first magnet 49 is in sealing sliding connection with the partition plates 44, a sealing hole is positioned above the first magnet 49, and the lower end of the sealing cover 11 is provided with starting pieces matched with a plurality of automatic adjusting assemblies;
in the initial state, the mounting plate 47 is positioned at the bottom of the mounting groove in the third spring 40, the first magnet 49 is further away from the top of the placing plate 21, and then the sealing holes are leaked, so that the sealing holes on two sides of the placing plate 21 are communicated with the empty groove 23 to facilitate ventilation, meanwhile, after the use is completed, the side surface of the placing plate 21 is placed to enable residual liquid in the empty groove 23 to be discharged from the sealing holes, the elastic force of the fourth spring 50 supports the first magnet 49 to extend out of the fixing frame 48, the transverse plate 43 is placed at the upper end of the convex strip 27 in use, at the moment, the third spring 40 and the first spring 26 are in balance state, the placing plate 21 leaks out of the upper side of the PCR detector body 1, so that the sealing cover 11 moves downwards to drive the third spring 40 with smaller elastic coefficient to preferentially compress the third spring 40 and the supporting plate 20 enters the slot 24, further, the mounting plate 47, the fixing frames 48 and the first magnets 49 are moved upwards to enable the first magnets 49 to abut against the empty slots 23, the first magnets 49 are used for blocking the sealing holes, then the raised strips 27 and the first springs 26 are compressed, further the sliding plate 29 is moved until the sealing cover 11 abuts against the upper cover of the detection test tube to move the compression starting piece, the sealing cover 11 is in sealing connection with the PCR detector body 1, the corresponding first magnets 49 are started to move to enable the sealing holes to leak out, detection operation is started, after detection is completed, the sealing cover 11 is opened, the resilience force of the first springs 26 and the third springs 40 can drive the placing plate 21 to move upwards, and further the placing plate 21 is enabled to leak out, so that the placing plate 21 is convenient to take.
The starting piece comprises an abutting plate 51 fixedly connected with the lower end of the sealing cover 11, the abutting plate 51 is provided with a plurality of sealing grooves 52 matched with test tube holes, sealing plates 53 which are flush with the abutting plate 51 are hermetically and slidingly connected in the sealing grooves 52, a plurality of fifth springs 54 are fixedly connected in the sealing grooves 52, step grooves are symmetrically formed in the abutting plate 51 in a left-right mode, two step grooves are internally and hermetically connected with a second magnet 55, a plurality of first magnets 49 and a plurality of second magnets 55 are close to each other in the same level, and the sealing grooves 52 are communicated with the step grooves and are provided with through grooves; when the sealing cover 11 moves downwards, the sealing plate 53 is matched with the test tube hole, the upper cover of the sealing plate is protruded to be placed on the placing plate 21 to be higher than the height of the placing plate 21 when the test tube is placed in the test tube hole, then the sealing plate 53 moves upwards against the resilience force of the fifth spring 54, then the air in the sealing plate 52 moves towards the step groove through the through groove, and the sealing groove 52 is in sealing connection with the step groove, so that the sealing groove 52 leads the air to the step groove under the movement of the sealing plate 53 and pushes the second magnet 55 to move downwards to be flush with the abutting plate 51, the repulsive force of the second magnet 55 and the first magnet 49 drives the first magnet 49 to compress the fourth spring 50 to move downwards to leak the sealing hole to be communicated with the joint 35 when the sealing plate 53 moves downwards, so that the solution can enter the independent cavity 44 for temperature control use, and the sealing plate 53 does not move downwards under the action of the fifth spring 54 when the transverse placing plate 21 for detecting the test tube is placed, and then the second magnet 55 cannot be driven to move downwards, so that the first magnet 55 can avoid the water inlet region from being blocked by the first magnet 49.
The number of each transverse row of the sealing holes is 8, the elastic sealing piece 22 is made of rubber, and the diameter size of the upper end of the elastic sealing piece 22 is larger than that of the lower end; the common PCR detection test tube is single tube or 8 allies oneself with the pipe, can conveniently place the detection test tube of 8 allies oneself with the pipe like this and a self-regulating assembly assorted, and then heats or the cooling through a self-regulating assembly when detecting 8 allies oneself with the pipe, improves the control by temperature change speed.
So make the elastic sealing member 22 expand and will detect the test tube and carry out the centre gripping after solution gets into the hollow 23 inside, and the diameter size of elastic sealing member 22 upper end is greater than the diameter size of lower extreme more adaptation and detects the thick shape of lower extreme of test tube upper end, and the biggest deformation state of elastic sealing member 22 can not shelter from the test tube hole completely yet simultaneously.
The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims of this invention, which are within the skill of those skilled in the art, can be made without departing from the spirit and scope of the invention disclosed herein.
Claims (8)
1. The utility model provides a PCR detection device, includes the PCR detector body, sealed lid is installed to PCR detector body upper end, PCR detector body internally mounted has detection mechanism and temperature control mechanism, touch control screen is installed to PCR detector body front side, PCR detector body internally mounted has the test tube rack, touch control screen and sealed lid, detection mechanism and temperature control mechanism electric connection, its characterized in that: the temperature control mechanism comprises a refrigerating assembly and a heating assembly, two water storage tanks are arranged in the PCR detector body, the refrigerating assembly and the heating assembly are respectively arranged in the two water storage tanks, the test tube placement frame comprises two support plates and a placement plate, the two support plates are symmetrically and fixedly connected to the lower end of the placement plate, the placement plate is provided with a plurality of test tube holes in a penetrating way, the lower sides of the test tube holes are all fixedly connected with elastic sealing elements in an annular way, empty slots communicated with the elastic sealing elements are formed in the placement plate, and the lower ends of the placement plate are symmetrically provided with the elastic elements;
the PCR detector is characterized in that slots matched with the supporting plate and the elastic piece are formed in the PCR detector body, a plurality of first springs matched with the elastic piece are symmetrically and fixedly connected in the slots, two convex strips are fixedly connected to the upper ends of the first springs and are in sliding connection with the slots, two pull ropes are symmetrically and fixedly connected to the lower ends of the convex strips, two pull ropes are arranged on the left side, two sliding plates are fixedly connected to the pull ropes and are in sliding connection with the PCR detector body respectively, two sliding plates are mutually far away from one side, a second spring is fixedly connected to the two sliding plates, a water inlet pipe is communicated with the left side of the heating tank in a penetrating mode, a communicating pipe is arranged on the water inlet pipe, two water outlet pipes are respectively communicated with the right side of the heating tank in a penetrating mode, water pumps are respectively arranged on the water outlet pipes and the communicating pipe, joints are respectively arranged on the water outlet pipes and the communicating pipe, sealing holes are formed in the placing plates and are in sealing connection with the joints, and a separating mechanism is arranged in the empty tank.
2. The PCR detection apparatus as claimed in claim 1, wherein: the elastic piece comprises a plurality of third springs fixedly connected to the lower end of the placing plate, a plurality of trapezoid pieces fixedly connected to the same side with the third springs are connected with the supporting plate in a sliding mode, and the lower ends of the trapezoid pieces are flush with the lower end of the placing plate.
3. The PCR detection apparatus as claimed in claim 2, wherein: the trapezoid piece comprises two inclined plates and a transverse plate, wherein the transverse plate is in sliding connection with the placing plate, the inclined plates are symmetrically and fixedly connected with the two sides of the transverse plate, and the transverse plate is matched with the convex strips.
4. A PCR detection apparatus according to claim 3, wherein: the separation mechanism comprises a plurality of baffles, wherein the baffles are uniformly and fixedly connected with the inside of the empty groove, the baffles and the empty groove form a plurality of independent cavities, the independent cavities are matched with the test tube holes, the sliding plates are mutually far away from one side and are respectively provided with a water diversion pipe, the two water diversion pipes are respectively communicated with the communicating pipe and the two water inlet pipes, the number and the positions of the connectors are matched with the independent cavities, and the inside of each independent cavity is provided with an automatic adjusting component.
5. The PCR detection apparatus according to claim 4, wherein: the two mounting grooves are formed in the supporting plates, the two trapezoid pieces are fixedly connected with mounting plates in sliding connection with the mounting grooves, the automatic adjusting assembly comprises a fixed frame fixedly connected with the upper end of the mounting plates, a first magnet is fixedly connected with the inner portion of the fixed frame in a sealing sliding manner, a fourth spring is fixedly arranged between the first magnet and the mounting plates, the first magnet is in sealing sliding connection with the partition plates, the sealing holes are located above the first magnet, and starting pieces matched with the automatic adjusting assemblies are arranged at the lower ends of the sealing covers.
6. The PCR detection apparatus according to claim 5, wherein: the starting piece comprises a butt plate fixedly connected with the lower end of a sealing cover, a plurality of sealing grooves matched with test tube holes are formed in the butt plate, sealing plates which are flush with the butt plate are connected in a sealing sliding mode in the sealing grooves, a plurality of fifth springs are fixedly connected in the sealing grooves, step grooves are formed in the butt plate in bilateral symmetry mode, two step grooves are formed in the step grooves in a sealing sliding mode, second magnets are connected in the step grooves in a sealing sliding mode, a plurality of first magnets and a plurality of second magnets are mutually close to one another, and through grooves are formed in the sealing grooves and the step grooves in a communicating mode.
7. The PCR detection apparatus as claimed in claim 6, wherein: the number of each transverse row of the sealing holes is 8, the elastic sealing piece is made of rubber, and the diameter size of the upper end of the elastic sealing piece is larger than that of the lower end of the elastic sealing piece.
8. The method for PCR detection according to claim 7, wherein: the method comprises the following steps:
step 1: the test tube to be detected is placed in the test tube hole on the test tube placing rack, and then the detection test tube is completely located in the test tube hole.
Step 2: the placed test tube rack is placed inside the PCR detector body, and then the raised strips are used for limiting and aligning the elastic pieces, so that the sealing cover is convenient to align the test tube rack for closing.
Step 3: after the sealing cover is aligned, sealed and closed, the connectors are in sealing connection with the sealing holes, and then the solution in the water storage tank is pumped by the water pump to enter the independent cavity for heating or cooling.
Step 4: when the temperature is required to rise, the water storage tank connected with the heating component works corresponding to the water pump of the water outlet pipe and the water pump of the communicating pipe, so that hot water heated by the circulating suction heating component enters the independent cavity to heat the detection test tube;
when cooling is needed, the water storage tank with the refrigeration component is connected with the water pump of the corresponding water outlet pipe and the water pump of the communicating pipe to work, so that the solution in the water storage tank enters the independent cavity to cool the detection test tube after the refrigeration of the refrigeration component is pumped during cooling; and the whole process continuously sucks the solution in the independent cavity to be in a circulating replacement state.
Step 5: and analyzing the PCR product after the reaction by a detection mechanism, and finally obtaining a result.
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| CN202311134608.9A CN117143723A (en) | 2023-09-05 | 2023-09-05 | PCR detection device and method |
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