CN115500262A

CN115500262A - Thermostatic type tea-oil camellia tissue culture seedling breeds and uses culture apparatus

Info

Publication number: CN115500262A
Application number: CN202211226888.1A
Authority: CN
Inventors: 申家朋; 张毕阳; 何辉; 王杨; 王辉; 董楠; 周成博; 王震; 王晶; 梁祥鹏
Original assignee: Xinyang Agriculture and Forestry University
Current assignee: Xinyang Agriculture and Forestry University
Priority date: 2022-10-09
Filing date: 2022-10-09
Publication date: 2022-12-23
Anticipated expiration: 2042-10-09
Also published as: CN115500262B

Abstract

The invention discloses a constant-temperature culture device for camellia oleifera tissue culture seedling propagation, which comprises an incubator, wherein ten first slide rails are symmetrically arranged on two sides inside the incubator, ten second slide rails are arranged on one adjacent side inside the incubator, and slide blocks are respectively connected inside the ten first slide rails and the ten second slide rails in a sliding manner.

Description

Thermostatic type tea-oil camellia tissue culture seedling breeds and uses culture apparatus

Technical Field

The invention relates to the technical field of oil tea tissue culture, in particular to a constant-temperature culture device for oil tea tissue culture seedling propagation.

Background

Tissue culture, also called tissue culture of higher plants, refers to a technique of separating one or a plurality of somatic cells or a part of plant bodies for culture, the tissue culture technique has a wide application range, is also applied in the process of oil tea tissue culture seedling propagation, and needs a special culture device for culture in the process of oil tea tissue culture seedling propagation.

Present oil tea tissue culture seedling breeds with culture apparatus cultivates the in-process, need put the blake bottle in the incubator one by one neatly, need take out one by one after the cultivation is accomplished, waste time and energy, greatly reduced work efficiency, and at the in-process of getting and putting, the condition of knocking over the blake bottle very easily appears, the security of taking has been reduced, oil tea tissue culture seedling is at the in-process of propagating, it is higher to the temperature requirement, current equipment is difficult to guarantee the invariant of equipment internal temperature, be unfavorable for the reproduction of oil tea tissue culture seedling, at the reproduction in-process of oil tea tissue culture seedling simultaneously, need artifical regular moisturizing, work efficiency is reduced, the cost of labor has been increased.

Disclosure of Invention

The invention aims to provide a constant-temperature culture device for oil-tea camellia tissue culture seedling propagation, which aims to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a thermostatted tea-oil camellia tissue culture seedling breeds and uses culture apparatus, includes the incubator, ten first slide rails are installed to the inside bilateral symmetry of incubator, and the inside adjacent one side of incubator installs ten second slide rails, and the inside equal sliding connection of ten first slide rails and ten second slide rails has the slider, and ten solid mobile fixed establishment are installed to the relative one side of twenty sliders, and the incubator is installed to incubator top one side, and the inside top surface central mounting of incubator has the heating pipe, incubator one side bottom fixedly connected with automatic water supply mechanism.

Preferably, the moving block comprises a movable plate, a movable block, a slot, a center hole, limiting holes, a clamping groove, a limiting sleeve, a guide rod, a spring and a guide groove, wherein ten movable plates are fixedly connected to one side of twenty sliding blocks, a plurality of limiting holes are uniformly formed in the top of each movable plate, the slot is formed in one side of each limiting hole, the clamping groove is formed in the bottom of each slot, the spring is installed in the center of the bottom of each limiting hole, the movable block is fixedly connected to the top of each spring, and the center hole is formed in the center of each movable block.

Preferably, two guide rods are symmetrically installed at the two sides of the center of the bottom of the limiting hole, two limiting sleeves are movably sleeved at the tops of the two guide rods, two guide grooves are formed in the centers of the bottoms of the two limiting sleeves, the inner circumferences of the two guide grooves and the outer circumferences of the two guide rods are attached to each other, the tops of the two limiting sleeves and the two sides of the bottom of the movable block are fixedly connected, a culture bottle is attached to the top of the movable block, a check valve is arranged in the center of the bottom of the culture bottle, a sealing ring is installed inside the check valve, a clamping block is installed on one side of the peripheral bottom of the culture bottle, and the clamping block is clamped inside the clamping groove.

Preferably, automatic water supply mechanism includes that the water delivery is responsible for, the manifold, communicating pipe, the rubber sleeve, first shunt tube, the second shunt tube, the intubate, the storage water tank, water pump and connecting pipe, the storage water tank is installed to incubator one side bottom, and storage water tank bottom one side installs the water pump, water pump output end fixedly connected with connecting pipe, and connecting pipe center one end runs through incubator one side bottom, connecting pipe one end fixedly connected with water delivery is responsible for, and the water delivery is responsible for bilateral symmetry and installs two manifolds, and two communicating pipes are installed at two manifold tops, two rubber sleeve are installed to two manifold one end, eight rubber sleeve are installed to two communicating pipe one side, ten first shunt tubes are installed to ten rubber sleeve one ends, ten first shunt tubes are installed inside the movable plate.

Preferably, ten equal fixedly connected with four second shunt tubes in first shunt tube both ends, and ten first shunt tubes and forty inside intercommunications of second shunt tubes, forty equal fixedly connected with two intubate in second shunt tube top.

Preferably, the incubator opposite side is articulated to be fixed with two chamber doors, and just two chamber doors one side centers all install the observation window, and four supporting legs are installed to incubator bottom four corners position, and a plurality of fluorescent lamps are installed to the inside bilateral symmetry of incubator, and install thermodetector on one side of the inside top surface of incubator.

Preferably, the ventilation hole has been seted up at thermostated container bottom center, and the ventilation hole is linked together with the incubator is inside, and the fan is installed to the inside bottom surface of thermostated container, and the inside one side of thermostated container installs the controller, and a plurality of louvres have evenly been seted up at the thermostated container top.

Compared with the prior art, the invention has the beneficial effects that:

1. according to the invention, through the installation of the integral moving and fixing mechanism, the culture bottles are fixed on the movable plate by utilizing the clamping blocks and the clamping grooves, and the plurality of culture bottles are simultaneously fixed on the movable plate without being put in and taken out one by one, so that the working efficiency is improved, the falling condition caused by collision is avoided, and the safety during moving and taking is improved;

2. according to the automatic water replenishing device, automatic water replenishing is realized through the installation of the automatic water replenishing mechanism, the insertion pipe is inserted into the one-way valve at the bottom of the culture bottle along with the downward pressing and fixing of the culture bottle, the automatic water replenishing is convenient to realize the automatic water replenishing from the bottom, the absorption of seedlings is facilitated, and meanwhile, compared with the traditional water replenishing mechanism, the automatic water replenishing mechanism is ingenious in design and small in size;

3. the temperature detector is used for detecting the temperature inside the incubator, when the temperature is higher than the set temperature, the controller opens the fan to perform air draft and heat dissipation until the temperature reaches the equipment temperature, and when the temperature is lower than the set temperature, the heating pipe is opened until the temperature reaches the set temperature and then is stopped, so that the temperature inside the incubator is kept constant.

Drawings

FIG. 1 is a perspective view of the overall construction of the present invention;

FIG. 2 is an elevational view of the overall construction of the present invention;

FIG. 3 is a front cross-sectional view of the overall construction of the present invention;

FIG. 4 is an enlarged view of area A of FIG. 3 according to the present invention;

FIG. 5 is an enlarged view of area B of FIG. 3 according to the present invention;

FIG. 6 is a perspective view of the integral moving and fixing mechanism of the present invention;

FIG. 7 is a front cross-sectional view of the unitary motion securing mechanism of the present invention;

FIG. 8 is an enlarged view of area C of FIG. 7 in accordance with the present invention;

FIG. 9 is a top cross-sectional view of the moving mass fixation mechanism of the present invention;

FIG. 10 is a perspective view of a culture flask according to the present invention;

FIG. 11 is a side partial cross-sectional view of the overall construction of the present invention;

in the figure: 1. an incubator; 2. an automatic water replenishing mechanism; 3. supporting legs; 4. a box door; 5. a thermostat; 6. an observation window; 7. an integral moving and fixing mechanism; 8. a culture flask; 9. heating a tube; 10. a temperature detector; 11. a first slide rail; 12. a slider; 13. a fluorescent lamp; 14. a second slide rail; 15. a seal ring; 16. a one-way valve; 17. a clamping block; 18. a suction hole; 19. a controller; 20. heat dissipation holes; 21. a fan; 701. moving the plate; 702. a movable block; 703. inserting slots; 704. a central bore; 705. a limiting hole; 706. a card slot; 707. a limiting sleeve; 708. a guide bar; 709. a spring; 710. a guide groove; 201. a water delivery main pipe; 202. a manifold; 203. a communicating pipe; 204. a rubber sleeve; 205. a first shunt pipe; 206. a second shunt pipe; 207. inserting a tube; 208. a water storage tank; 209. a water pump; 210. and (4) connecting the pipes.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

Referring to fig. 1-11, an embodiment of the present invention: a constant-temperature culture device for oil tea tissue culture seedling propagation comprises an incubator 1, ten first slide rails 11 are symmetrically arranged on two sides in the incubator 1, ten second slide rails 14 are arranged on one adjacent side in the incubator 1, sliders 12 are respectively and slidably connected in the ten first slide rails 11 and the ten second slide rails 14, ten integrally moving fixing mechanisms 7 are arranged on one opposite sides of the twenty sliders 12, each integrally moving fixing mechanism 7 comprises a moving plate 701, a moving block 702, a slot 703, a central hole 704, a limiting hole 705, a clamping groove 706, a limiting sleeve 707, a guide rod 708, a spring 709 and a guide groove 710, ten moving plates 701 are fixedly connected on one opposite sides of the twenty sliders 12, a plurality of limiting holes 705 are uniformly arranged on the top of each moving plate 701, the slot 703 is arranged on one side of each limiting hole 705, the clamping groove 706 is arranged at the bottom of each slot 703, and the spring 709 is arranged at the center of the bottom of each limiting hole 705, and the top of the spring 709 is fixedly connected with a movable block 702, the center of the movable block 702 is provided with a central hole 704, two guide rods 708 are symmetrically arranged on two sides of the bottom center of the limiting hole 705, two limiting sleeves 707 are movably sleeved on the tops of the two guide rods 708, two guide grooves 710 are arranged in the centers of the bottoms of the two limiting sleeves 707, the inner circumferences of the two guide grooves 710 are attached to the outer circumferences of the two guide rods 708, the tops of the two limiting sleeves 707 are fixedly connected with two sides of the bottom of the movable block 702, the top of the movable block 702 is attached with a culture bottle 8, the center of the bottom of the culture bottle 8 is provided with a one-way valve 16, a sealing ring 15 is arranged in the one-way valve 16, a fixture block 17 is arranged on one side of the peripheral bottom of the culture bottle 8, the fixture block 17 is clamped in a clamping groove 706, the culture bottle 8 is conveniently moved and fixed by an integral moving fixing mechanism 7, and a constant temperature box 5 is arranged on one side of the top of the culture box 1, the center of the top surface in the incubator 1 is provided with a heating pipe 9, the bottom of one side of the incubator 1 is fixedly connected with an automatic water replenishing mechanism 2, the automatic water replenishing mechanism 2 comprises a water delivery main pipe 201, a manifold 202, a communicating pipe 203, a rubber sleeve 204, a first shunt pipe 205, a second shunt pipe 206, an insertion pipe 207, a water storage tank 208, a water pump 209 and a connecting pipe 210, the bottom of one side of the incubator 1 is provided with the water storage tank 208, one side of the bottom of the water storage tank 208 is provided with the water pump 209, the output end of the water pump 209 is fixedly connected with the connecting pipe 210, one end of the center of the connecting pipe 210 penetrates through the bottom of one side of the incubator 1, one end of the connecting pipe 210 is fixedly connected with the water delivery main pipe 201, two manifolds 202 are symmetrically arranged on two sides of the water delivery main pipe 201, the tops of the two manifolds 203 are provided with two communicating pipes 203, one end of the two manifolds 202 is provided with two rubber sleeves 204, one side of the two communicating pipes 203 is provided with eight rubber sleeves 204, ten first shunt tubes 205 are installed at one end of ten rubber sleeves 204, the ten first shunt tubes 205 are installed inside a moving plate 701, four second shunt tubes 206 are fixedly connected to two ends of each of the ten first shunt tubes 205, the ten first shunt tubes 205 are communicated with the insides of the forty second shunt tubes 206, two insertion tubes 207 are fixedly connected to the tops of the forty second shunt tubes 206, automatic water replenishing is facilitated, two box doors 4 are fixedly hinged to the other side of an incubator 1, observation windows 6 are installed at the centers of one sides of the two box doors 4, four support legs 3 are installed at four corners of the bottom of the incubator 1, a plurality of fluorescent lamps 13 are symmetrically installed at two sides inside the incubator 1, a temperature detector 10 is installed at one side of the top surface inside the incubator 1, cultivation conditions can be observed through the observation windows 6, and an air exhaust hole 18 is formed in the center of the bottom of the incubator 5, and air draft hole 18 is linked together with incubator 1 is inside, and fan 21 is installed to the inside bottom surface of thermostated container 5, and controller 19 is installed to inside one side of thermostated container 5, and four louvres 20 have evenly been seted up at thermostated container 5 top.

The working principle is as follows: in the using process of the invention, firstly, seedlings to be subjected to tissue culture seedling propagation are inoculated into a culture bottle 8, then the culture bottles 8 are fixed on an integral moving fixing mechanism 7 one by one, the fixture blocks 17 at the bottom of the culture bottle 8 are aligned with the slots 703, then the culture bottle 8 is pressed downwards along the limiting holes 705, at this time, the movable block 702 is driven to move downwards, then the limiting sleeve 707 is driven to move downwards along the guide rod 708, the guide rod 708 and the limiting sleeve 707 are used for ensuring the moving direction of the movable block 702, in the downward moving process, the springs 709 are compressed, when the fixture blocks 17 move to the bottom of the slots 703, at this time, the culture bottle 8 is rotated, the fixture blocks 17 are driven to rotate, the fixture blocks 17 are rotated into the clamping grooves 706, under the pressure of the springs 709, the clamping and fixing of the culture bottles 8 are realized, the culture bottles 8 are fixed on the moving plate 701 one by one, the fixing of the culture bottles 8 is favorable for the fixed installation of the culture bottles 8, when the culture bottle 8 is fixed, the insertion tube 207 penetrates through the central hole 704 and is inserted into the check valve 16 at the bottom of the culture bottle 8 to communicate the culture bottle 8 and the insertion tube 207, the sealing ring 15 is used for ensuring the sealing between the check valve 16 and the insertion tube 207, after the culture bottle 8 is fixed, the box door 4 of the culture box 1 is opened, the sliding blocks 12 at two sides of the moving plate 701 are respectively inserted into the first sliding rail 11 and the second sliding rail 14, so that the moving plate 701 is quickly fixed, the culture bottles 8 are conveniently placed into the culture box 1 at one time without being taken out and placed one by one, the working efficiency is improved, along with the insertion of the moving plate 701, the first shunt tube 205 is directly inserted into the rubber sleeve 204, at the moment, the box door 4 is closed, the fluorescent lamp 13 is opened to carry out tissue culture propagation, the temperature detector 10 automatically detects the temperature inside the culture box 1, and when the temperature inside the culture box 1 is higher than the set temperature, at the moment, the controller 19 in the incubator 5 sends an electric signal, the fan 21 is turned on, the fan 21 starts to work, hot air in the incubator 1 is pumped out through the air pumping hole 18 and is discharged through the heat dissipation hole 20, the temperature of the incubator 1 is reduced, when the set temperature value is reached, the fan 21 stops working, when the temperature bottom is set, the controller 19 sends an electric signal, the heating pipe 9 is turned on, the heating pipe 9 starts to heat the inside of the incubator 1 until the temperature in the incubator 1 reaches the set value, the temperature in the incubator 1 is constant, and the propagation of camellia oleifera tissue culture seedlings is facilitated, when water replenishing is needed, the water pump 209 is turned on, the water pump 209 starts to work, water in the water storage tank 208 is input into the water conveying main pipe 201 through the connecting pipe 210, then is input into the manifold 202, then is output into the communicating pipe 203, then is input into the first flow dividing pipe 205 through the rubber sleeve 204, then is input into the second flow dividing pipe 206, then is input into the insertion pipe 207, then enters into the culture bottle 8 through the check valve 16, the automatic water replenishing of the culture bottle 8 is realized, and is different from the novel drip irrigation support tube design, the incubator 1, the observation of the drip irrigation support for observing the culture box 1, and the conditions of the culture box 1 are convenient for observing.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a constant temperature formula tea-oil camellia tissue culture seedling breeds and uses culture apparatus, includes incubator (1), its characterized in that: ten first slide rails (11) are installed to the inside bilateral symmetry of incubator (1), and incubator (1) inside adjacent one side installs ten second slide rails (14), ten first slide rails (11) and ten second slide rails (14) inside equal sliding connection have slider (12), and ten solid mobile fixture (7) are installed to the relative one side of twenty slider (12), incubator (5) are installed to incubator (1) top one side, and incubator (1) inside top surface center installs heating pipe (9), incubator (1) one side bottom fixedly connected with automatic water supply mechanism (2).

2. The culture apparatus for propagation of tissue culture seedling of camellia oleifera as set forth in claim 1, wherein: the integral moving fixing mechanism (7) comprises a moving plate (701), a moving block (702), slots (703), a center hole (704), limiting holes (705), clamping grooves (706), limiting sleeves (707), guide rods (708), springs (709) and guide grooves (710), ten moving plates (701) are fixedly connected to one side of twenty sliding blocks (12) opposite to each other, a plurality of limiting holes (705) are uniformly formed in the top of the moving plate (701), the slots (703) are formed in one side of the limiting holes (705), the clamping grooves (706) are formed in the bottoms of the slots (703), the springs (709) are installed in the centers of the bottoms of the limiting holes (705), the moving block (702) is fixedly connected to the tops of the springs (709), and the center hole (704) is formed in the center of the moving block (702).

3. The culture apparatus for propagation of tissue culture seedling of camellia oleifera as set forth in claim 2, wherein: two guide bars (708) are installed to spacing hole (705) bottom center bilateral symmetry, and two guide bar (708) top activity cup joints two spacing sleeves (707), two guide way (710) have been seted up at two spacing sleeve (707) bottom centers, and the laminating of circumference and two guide bar (708) outer circumferences in two guide way (710), two spacing sleeve (707) tops and movable block (702) bottom both sides fixed connection, movable block (702) top laminating has blake bottle (8), and blake bottle (8) bottom center is provided with check valve (16), check valve (16) internally mounted has sealing washer (15), fixture block (17) are installed to blake bottle (8) peripheral bottom one side, and fixture block (17) joint is inside draw-in groove (706).

4. The culture apparatus for propagation of tissue culture seedling of camellia oleifera as set forth in claim 1, wherein: automatic moisturizing mechanism (2) are responsible for (201) including the water delivery, manifold (202), communicating pipe (203), rubber sleeve (204), first shunt pipe (205), second shunt pipe (206), intubate (207), storage water tank (208), water pump (209) and connecting pipe (210), storage water tank (208) are installed to incubator (1) one side bottom, and water pump (209) are installed to storage water tank (208) bottom one side, water pump (209) output end fixedly connected with connecting pipe (210), and connecting pipe (210) center one end runs through incubator (1) one side bottom, connecting pipe (210) one end fixedly connected with water delivery is responsible for (201), and water delivery is responsible for (201) bilateral symmetry and installs two manifold (202), and two communicating pipe (203) are installed at two manifold (202) tops, two rubber sleeve (204) are installed to two manifold (202) one end, eight rubber sleeve (204) are installed to two communicating pipe (203) one side, ten rubber sleeve (204) one end are installed first shunt pipe (205), first shunt pipe (205) is installed inside movable plate (701).

5. The culture apparatus for propagation of tissue culture seedling of camellia oleifera as set forth in claim 4, wherein: ten equal fixedly connected with four second shunt tubes (206) in first shunt tube (205) both ends, and ten first shunt tubes (205) and forty second shunt tubes (206) inside intercommunication, two intubate (207) of the equal fixedly connected with in forty second shunt tubes (206) top.

6. The culture apparatus for propagation of tissue culture seedling of camellia oleifera as set forth in claim 1, wherein: the incubator (1) is hinged to be fixed with two box doors (4) on the other side, an observation window (6) is installed at the center of one side of each of the two box doors (4), four supporting legs (3) are installed at four corners of the bottom of the incubator (1), a plurality of fluorescent lamps (13) are installed in bilateral symmetry inside the incubator (1), and a temperature detector (10) is installed on one side of the top surface inside the incubator (1).

7. The culture apparatus for propagation of tissue culture seedling of camellia oleifera as set forth in claim 1, wherein: thermostat (5) bottom center has seted up convulsions hole (18), and convulsions hole (18) and incubator (1) inside be linked together, and fan (21) are installed to thermostat (5) inside bottom surface, and thermostat (5) inside one side installs controller (19), and a plurality of louvres (20) have evenly been seted up at thermostat (5) top.