CN111454840A

CN111454840A - Device for developing rabies vaccine by using self weight of culture

Info

Publication number: CN111454840A
Application number: CN202010254095.5A
Authority: CN
Inventors: 黄国莲
Original assignee: Shaoxing Dalengken Biotechnology Co ltd
Current assignee: Shaoxing Dalengken Biotechnology Co ltd
Priority date: 2020-04-02
Filing date: 2020-04-02
Publication date: 2020-07-28

Abstract

The invention relates to the technical field of rabies vaccine development, and discloses equipment for developing rabies vaccines by utilizing the dead weight of cultures, which comprises a box body, wherein a placing groove is fixedly connected with the box body, the bottom of the placing groove is movably connected with a liquid flow plate, the surface of the liquid flow plate is movably connected with a spring rod, the other end of the spring rod is movably connected with an elastic plate, a guide rail is fixedly connected inside the placing groove, the surface of the guide rail is movably connected with a retaining plate, the joint of the retaining plate and the guide rail is movably connected with a force storage spring, the surface of the force storage spring is movably connected with a pull rope, the other end of the pull rope is wound with a clamping column, the surface of the clamping column is movably clamped with a clamping groove, and a sliding plate is movably connected on. The board of exerting pressure of symmetry can be close to each other and extrude the reposition of redundant personnel piece, and the culture solution in the liquid storage pipe can be extruded earlier, and the original power of extrusion that receives because of the liquid storage pipe is the raw materials dead weight, so reached according to the automatic effect of adding proper amount culture dish of raw materials dead weight.

Description

Device for developing rabies vaccine by using self weight of culture

Technical Field

The invention relates to the technical field of rabies vaccine development, in particular to equipment for developing rabies vaccine by using the dead weight of a culture.

Background

The human refined cell rabies vaccine and the refined hamster kidney cell rabies vaccine are light turbid white liquid, dsRNA with the length exceeding thirty can cause the problem of interferon toxicity, the risk is worldwide, precipitate which can be shaken off is formed after long-term storage, and the preservative contains thimerosal; the process for developing the rabies vaccine comprises the following steps: cell preparation, virus inoculation, virus receiving and the like, wherein the inoculation work of the virus is important.

The culture dish is often used to the development of vaccine to prior art, but at the in-process of virus inoculation, it is higher to the surrounding environment's requirement because of the characteristic of virus, and traditional culture dish is at the in-process of inoculating the virus, need open the ware lid and pour out earlier its inside culture solution, add maintenance liquid afterwards, harvest again, can bring the microorganism in the air into the ware body between the ware lid opens and shuts, the growth characteristic of violating the virus, and the electron culture dish can produce the electric radiation and can not be used, consequently an utilize culture dead weight to develop rabies vaccine equipment and take place of life.

Disclosure of Invention

In order to realize the purposes that the inoculation culture work of the virus can be finished without opening the dish cover and a proper amount of culture dishes are automatically added according to the self weight of the raw materials, the invention provides the following technical scheme: the utility model provides an utilize culture dead weight to develop rabies bacterin equipment, the power distribution box comprises a box body, the fixedly connected with standing groove of box, the bottom swing joint of standing groove has the liquid to flow the board, and the surperficial swing joint that the liquid flowed the board has the spring beam, and the other end swing joint of spring beam has the elastic plate, the inside fixedly connected with guide rail of standing groove, and the surperficial swing joint of guide rail has the fender and keeps the board, keeps off the board and has the power spring with the junction swing joint of guide rail, the surperficial swing joint who keeps the power spring has the stay cord, and the other end winding of stay cord has the card post, and the surperficial swing joint of card post has the draw-in groove, and the surperficial sliding connection of slide that just is located the draw-in groove has the clamp plate, and the.

The invention has the beneficial effects that:

1. inside putting into the box through the raw materials that will prepare the cell, back card post removes to the direction that is close to the box, so the board of exerting pressure of symmetry can be close to each other and extrude the reposition of redundant personnel piece, and the culture solution in the liquid storage tube can be extruded earlier, because of the original power of extrusion that the liquid storage tube received is the raw materials dead weight, so reached according to the automatic effect of adding proper amount culture dish of raw materials dead weight.

2. Through adding the standing groove with the culture solution, the culture solution has also reached the requirement with the culture time of raw materials, and along with the continuous play of culture solution, the weight that keeps off holding plate surface and receive diminishes gradually, and the board of exerting oneself elasticity restoring force of clamp plate by the gasbag promotes, and the maintenance liquid in the arc pipe is sucked to the stock solution pipe in, and the back is released from the drain pipe, so reached need not to open the effect that the inoculation of dish lid alright completion virus cultivateed the work.

Preferably, the shunt part comprises an air bag, the surface of the air bag is fixedly connected with an arc-shaped pipe, and the bending angle of the arc-shaped pipe is sixty degrees.

Preferably, the fixed surface of slide is connected with the drain pipe, and the drain pipe runs through the inside of reposition of redundant personnel piece, and is for the intercommunication with the standing groove, is convenient for flow the culture solution extrusion in the liquid storage pipe into the standing groove.

Preferably, the end of the power storage spring, which is far away from the retaining plate, is hinged with the box body, a hollow sleeve is arranged at the hinged position, the depth of the hollow sleeve is matched with the length of the power storage spring, and a certain space is provided for the deformation and displacement of the power storage spring due to the existence of the hollow sleeve, so that the power storage spring is prevented from being excessively extruded and deformed.

Preferably, the movable angle of the retaining plate is thirty degrees to one hundred twenty degrees, the airflow pipe is movably connected inside the retaining plate, the airflow pipe works on the principle of expansion with heat and contraction with cold, and the flowing culture fluid has a certain temperature, so that the airflow pipe expands and expands to push the spring rod to work.

Preferably, the number of the flow plates is two, a fixed gap is reserved between the symmetrical flow plates, and the width of the gap is controlled by the spring rod.

Preferably, the elastic plate is arranged at the lower half part of the guide rail, the retaining plate is in contact with the elastic plate when the retaining plate is at thirty degrees, and when the retaining plate is in contact with the elastic plate, the elastic plate is extruded to push the symmetrical spring rods to approach each other, so that the symmetrical liquid flow plates approach each other to reduce gaps, and raw materials falling from the lower part of the culture liquid are prevented.

Preferably, the inside of casing just is located the below fixedly connected with storage tank that the liquid flows the board, and the inside of storage tank is the layering design, is convenient for deposit culture solution and a small amount of raw materials that drop.

Drawings

FIG. 1 is a front sectional view of the box structure of the present invention;

FIG. 2 is a schematic view of a retainer plate according to the present invention;

FIG. 3 is a schematic view of the spring rod structure of the present invention;

FIG. 4 is a schematic view of the pull cord of the present invention;

FIG. 5 is a schematic view of the structure of the placement groove of the present invention;

FIG. 6 is a schematic view of a liquid storage tube according to the present invention;

FIG. 7 is an enlarged view of a portion A of FIG. 6;

fig. 8 is a schematic view of the splitter of the present invention.

In the figure: 1-box body, 2-placing groove, 3-flow plate, 4-spring rod, 5-elastic plate, 6-guide rail, 7-retaining plate, 8-force-storing spring, 9-pull rope, 10-clamping column, 11-clamping groove, 12-sliding plate, 13-pressing plate, 14-liquid storage tube, 15-shunt, 16-air bag, 17-arc tube and 18-liquid outlet tube.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-8, an apparatus for developing rabies vaccine by using the self weight of a culture comprises a case 1, a fixedly connected placing groove 2 of the case 1, a liquid flow plate 3 movably connected to the bottom of the placing groove 2, a storage groove fixedly connected to the inside of the case 1 and below the liquid flow plate 3, wherein the storage groove is layered and is convenient for storing a culture solution and a small amount of dropped raw materials, spring rods 4 are movably connected to the surface of the liquid flow plate 3, the number of the liquid flow plates 3 is two, fixed gaps are left between the symmetrical liquid flow plates 3, and the width of the gaps is controlled by the spring rods 4.

The other end swing joint of spring beam 4 has elastic plate 5, elastic plate 5 is located the latter half of guide rail 6, keep off and hold board 7 and contact with elastic plate 5 when thirty degrees, when keeping off and holding board 7 and elastic plate 5 contact, the elastic plate extrusion and promote symmetrical spring beam 4 and be close to each other, so the liquid flow board 3 of symmetry is close to each other and reduces the gap, prevent to drop by the raw materials that the culture solution area comes down, the inside fixedly connected with guide rail 6 of standing groove 2, the surface swing joint of guide rail 6 has keeps off and holds board 7, the activity angle that keeps off and holds board 7 is thirty degrees to one hundred twenty degrees, and the inside swing joint that keeps off and hold board 7 has the air current pipe, the theory of operation of air current pipe is expend with heat and contract with cold, expend with heat and contract with cold indicates that the object can expand when being heated. Because the movement of particles (atoms) in the object can change along with the temperature, when the temperature rises, the vibration amplitude of the particles is increased, so that the object expands; when the temperature is reduced, the vibration amplitude of the particles is reduced, so that the object is contracted; when the culture solution flows into the culture solution, the culture solution has a certain temperature, so that the airflow tube expands and pushes the spring rod 4 to work.

Keep off the junction swing joint of board 7 and guide rail 6 and have a power spring 8, hold power spring 8 and keep away from the one end of keeping off board 7 articulated with box 1, and articulated department is provided with hollow cover, the degree of depth of hollow cover and the length looks adaptation of holding power spring 8, the existence of hollow cover provides certain space for holding power spring 8's deformation displacement, prevents to hold power spring 8 by excessive extrusion deformation.

The surface swing joint who holds power spring 8 has stay cord 9, the other end winding of stay cord 9 has card post 10, the surface swing joint of card post 10 has draw-in groove 11, the existence of draw-in groove 11 is for adjusting hold power spring 8 and exert pressure between the board 13, and then be convenient for reach the purpose according to raw materials dead weight control culture solution outflow, the right side swing joint who blocks the surface of post 10 and be located draw-in groove 11 has slide 12, slide 12's fixed surface is connected with drain pipe 18, drain pipe 18 runs through the inside of reposition of redundant personnel 15, and be intercommunication with standing groove 2, be convenient for extrude the culture solution in the stock solution pipe 14 and flow into standing groove 2.

The surface of the sliding plate 12 is connected with a pressing plate 13 in a sliding mode, a liquid storage pipe 14 is fixedly connected to the surface of the sliding plate 12 and located below the pressing plate 13, a flow dividing part 15 is movably connected to the surface of the liquid storage pipe 14, the flow dividing part 15 comprises an air bag 16, and two sides of the air bag 16 are in an intercommunicating design after being extruded, so that liquid can be sucked to the other side from one side by air flow generated under the action of self elastic restoring force, an arc-shaped pipe 17 is fixedly connected to the surface of the air bag 16, the bending angle of the arc-shaped pipe 17 is sixty degrees, the liquid outlet pipe 18 is just well positioned at the center of the arc-shaped pipe 17, and the vibration force generated.

When the device is used, raw materials for preparing cells are placed in the box body 1 and then fall on the surface of the retaining plate 7, the retaining plate 7 deflects from an original positive angle to a horizontal state under the action of pressure, the retaining plate 7 also deflects towards a negative angle along with the continuous addition of the raw materials, an airflow pipe in the retaining plate 7 is influenced by expansion with heat and contraction with cold in the deflection process, the deformation of the airflow pipe can push the power storage spring 8 to move towards the direction far away from the placing groove 2, the deformation generated in the extrusion process of the power storage spring 8 can pull the pull rope 9, the pull rope 9 is tight, the clamping column 10 can be pulled to move towards the direction close to the box body 1, so that the symmetrical pressure applying plates 13 can be close to each other and extrude the shunting piece 15, the shunting piece 15 is extruded, according to the relation between the pressure and the volume, the culture solution in the liquid storage pipe 14 with a larger volume can be extruded out firstly and flows towards the inside the placing groove 2 from the liquid outlet pipe 18, wherein the original extrusion force of the liquid storage pipe 14 is the dead weight of the raw materials.

By adding the culture solution into the placing groove 2, the weight on the surface of the baffle plate 7 is continuously increased along with the continuous addition of the culture solution, so the baffle plate 7 is gradually changed from a balanced state to a negative angle state, when the symmetrical baffle plate 7 deflects to the negative angle, a gap is formed between the two, meanwhile, the culture time of the culture solution and the raw material is also met, the flowing speed of the liquid is higher than that of the solid at the same flowing speed, so the culture solution flows out from the gap, the weight on the surface of the baffle plate 7 is gradually reduced along with the continuous outflow of the culture solution, so the culture solution is changed from the negative angle state to a horizontal state, when the baffle plate 7 is in the negative angle state, the extrusion force on the power storage spring 8 is smaller, the pull rope 9 is loosened, the pressure applying plate 13 is pushed by the self elastic restoring force of the air bag 16, and the characteristic of sucking the air by the elastic restoring force of the air bag 16 is utilized, the culture medium in the arc tube 17 is sucked into the liquid storage tube 14 and then pushed out from the liquid outlet tube 18, at which time the culture medium in the well 2 is drained.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.

Claims

1. The utility model provides an utilize culture dead weight to develop rabies vaccine equipment, includes box (1), its characterized in that: the box body (1) is fixedly connected with a placing groove (2), the bottom of the placing groove (2) is movably connected with a liquid flow plate (3), the surface of the liquid flow plate (3) is movably connected with a spring rod (4), the other end of the spring rod (4) is movably connected with an elastic plate (5), the interior of the placing groove (2) is fixedly connected with a guide rail (6), the surface of the guide rail (6) is movably connected with a retaining plate (7), and the joint of the retaining plate (7) and the guide rail (6) is movably connected with a power storage spring (8);

the surface swing joint of power storage spring (8) has stay cord (9), and the other end winding of stay cord (9) has card post (10), and the surface swing joint of card post (10) has draw-in groove (11), and the surface of card post (10) just is located the right side swing joint of draw-in groove (11) has slide (12), and the surperficial sliding connection of slide (12) has the board of exerting pressure (13), and the surface of slide (12) just is located the below fixedly connected with liquid storage pipe (14) of exerting pressure board (13), and the surface swing joint of liquid storage pipe (14) has reposition of redundant personnel piece (15).

2. The apparatus for developing rabies vaccine by culture dead weight according to claim 1, wherein: the flow dividing piece (15) comprises an air bag (16), the surface of the air bag (16) is fixedly connected with an arc-shaped pipe (17), and the bending angle of the arc-shaped pipe (17) is sixty degrees.

3. The apparatus for developing rabies vaccine by culture dead weight according to claim 1, wherein: the surface of the sliding plate (12) is fixedly connected with a liquid outlet pipe (18), and the liquid outlet pipe (18) penetrates through the inside of the flow dividing piece (15) and is communicated with the placing groove (2).

4. The apparatus for developing rabies vaccine by culture dead weight according to claim 1, wherein: one end, far away from the retaining plate (7), of the power storage spring (8) is hinged to the box body (1), a hollow sleeve is arranged at the hinged position, and the depth of the hollow sleeve is matched with the length of the power storage spring (8).

5. The apparatus for developing rabies vaccine by culture dead weight according to claim 1, wherein: the movable angle of the retaining plate (7) is thirty degrees to one hundred twenty degrees, and the interior of the retaining plate (7) is movably connected with an airflow pipe.

6. The apparatus for developing rabies vaccine by culture dead weight according to claim 1, wherein: the number of the liquid flow plates (3) is two, a fixed gap is reserved between the symmetrical liquid flow plates (3), and the width of the gap is controlled by the spring rod (4).

7. The apparatus for developing rabies vaccine by culture dead weight according to claim 1, wherein: the elastic plate (5) is arranged at the lower half part of the guide rail (6), and the retaining plate (7) is in contact with the elastic plate (5) when the retaining plate is arranged at thirty degrees.

8. The apparatus for developing rabies vaccine by culture dead weight according to claim 1, wherein: the inside of the shell (1) is fixedly connected with a storage tank below the liquid flow plate (3), and the storage tank is in layered design.