CN216594772U

CN216594772U - PCR (polymerase chain reaction) quantitative analyzer

Info

Publication number: CN216594772U
Application number: CN202122979795.3U
Authority: CN
Inventors: 刘福利; 沈锋杰; 汤建君
Original assignee: Wuxi Opulen Technology Co ltd
Current assignee: Wuxi Opulen Technology Co ltd
Priority date: 2021-11-30
Filing date: 2021-11-30
Publication date: 2022-05-24
Anticipated expiration: 2031-11-30

Abstract

The utility model discloses a PCR (polymerase chain reaction) quantitative analyzer, which comprises an analyzer, wherein a heating pool is arranged at the top of the analyzer, a heating support plate is clamped at the top of the heating pool, a heating sleeve is fixedly connected at the bottom of the heating support plate, a first flow channel and a second flow channel are arranged on the inner wall of the heating pool, a first pipe connector and a second pipe connector are fixedly connected to the back of the analyzer, one end of the first flow channel is communicated with the first pipe connector, and the second flow channel is communicated with the second pipe connector. According to the utility model, the first flow channel and the second flow channel are arranged on one side of the heating pool, the heating pool can be connected with the external steam generator by combining the first pipe connector and the second pipe connector, steam or cooling water is injected into the heating pool through the steam generator, so that the rapid temperature rise or reduction is realized, the detection and analysis efficiency of an instrument is improved, the temperature is monitored through the temperature sensor in the whole reaction process, and the reaction temperature is conveniently controlled.

Description

PCR (polymerase chain reaction) quantitative analyzer

Technical Field

The utility model relates to the technical field of analytical instruments, in particular to a PCR (polymerase chain reaction) quantitative analyzer.

Background

The gene amplification instrument has excellent performance and can meet various requirements of different working environments. Can be used for fluorescence detection and gene analysis of various pathogens characterized by polymerase chain reaction and aiming at fluorescence detection of DNA/RNA. The method is widely applied to the fields of molecular biology, medicine, food industry, judicial sciences, biotechnology, environmental science, microbiology, clinical diagnosis, epidemiology, genetics, gene chips, gene fluorescence detection, gene cloning, gene expression and the like. The PCR quantitative analyzer is added with a fluorescence detection system on the basis of the gene amplification instrument, realizes quantitative analysis by means of comparison between a sample and a standard substance, and obtains the initial concentration or content of the detected sample Z more intuitively.

When the existing PCR quantitative analyzer is used, the reaction temperature is inconvenient to control, and the temperature rise and the temperature fall need longer time, so that the detection and analysis efficiency of the analyzer is lower.

Therefore, it is necessary to invent a PCR quantitative analyzer to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a PCR quantitative analyzer, which solves the problems that the existing PCR quantitative analyzer is inconvenient to control the reaction temperature and needs longer time for temperature rise and temperature reduction, so that the detection and analysis efficiency of the analyzer is lower.

In order to achieve the above purpose, the utility model provides the following technical scheme: the utility model provides a PCR polymerase chain reaction quantitative analyzer, includes the analysis machine, the heating pond has been seted up at the analysis machine top, heating pond top joint is provided with the heating support plate, heating support plate bottom fixedly connected with heating sleeve pipe, first runner and second runner have been seted up to the heating pond inner wall, first pipe connector of analysis machine back fixedly connected with and second pipe connector, first runner one end is linked together with first pipe connector, the second runner is linked together with the second pipe connector, analysis machine back fixedly connected with hangs the spigot, it sets up in first pipe connector and second pipe connector bottom to hang the spigot, the analysis machine back is equipped with flourishing water bucket, flourishing water bucket one side has been seted up the card hole, hang the spigot and block the hole and articulate mutually.

Preferably, the heating sleeve bottom extends to inside the heating bath, the heating sleeve quantity is established to a plurality ofly, and is a plurality of heating sleeve evenly distributed, heating sleeve's the convenient reaction test tube that places that sets up.

Preferably, the inner wall of the heating pool is fixedly connected with a temperature sensor, the front surface of the analyzer is fixedly connected with a liquid crystal display screen, and the temperature sensor and the liquid crystal display screen are electrically connected with the analyzer.

Preferably, the analysis machine top is equipped with fluorescence detection mechanism, fluorescence detection mechanism includes the apron, apron and heating bath phase-match, apron bottom fixedly connected with fluorescence detector, fluorescence detector and analysis machine electric connection, temperature sensor can monitor reaction temperature, conveniently controls reaction temperature.

Preferably, the equal fixedly connected with slider in apron both sides, two the equal fixedly connected with stopper in slider one side, the analysis machine top fixedly connected with encloses and keeps off the frame, enclose and keep off frame inner wall both sides and all seted up the spout, two the stopper respectively with two spout sliding connection, the setting of stopper and spout can be spacing to the apron when making things convenient for the apron to remove, makes the apron can pinpoint in heating the pond top.

Preferably, one end of the top of the analyzer is fixedly connected with a control panel, the front of the analyzer is fixedly connected with a power switch, the control panel and the power switch are electrically connected with the analyzer, and the control panel is arranged to facilitate operation of an operator on the analyzer.

Preferably, one side of the analyzer is fixedly connected with a power interface, the power interface is electrically connected with the analyzer, and the bottom of the analyzer is fixedly connected with a bottom plate.

Preferably, bottom plate bottom fixedly connected with supporting legs, supporting legs quantity is established to four, four supporting legs evenly distributed is in bottom plate four corners, and the setting of supporting legs can carry out certain degree to the device and raise, keeps apart device and mesa.

In the technical scheme, the utility model provides the following technical effects and advantages:

1. the heating pool can be connected with an external steam generator by arranging the first flow channel and the second flow channel on one side of the heating pool and combining the first pipe connector and the second pipe connector, steam or cooling water is injected into the heating pool through the steam generator, so that the rapid temperature rise or reduction is realized, the detection and analysis efficiency of an instrument is improved, the temperature is monitored through a temperature sensor in the whole reaction process, and the reaction temperature is conveniently controlled;

2. monitoring reaction process through setting up fluorescence detection mechanism, will lapping and remove to heating bath top, lap the fluorescence detector of bottom and just in time be in the heating support plate top, lap simultaneously and provide the dark environment for fluorescence detector, make things convenient for fluorescence detector to carry out work.

Drawings

FIG. 1 is a schematic overall structure diagram of a first perspective of the present invention;

FIG. 2 is a cross-sectional view of the overall construction from a second perspective of the present invention;

FIG. 3 is a schematic view of the overall structure of the working state of the present invention;

FIG. 4 is a partial structural view of a first perspective of the present invention;

FIG. 5 is a partial structural view from a second perspective of the present invention;

FIG. 6 is a schematic view of the structure of the heating tank of the present invention;

FIG. 7 is a schematic structural view of a fluorescence detection mechanism according to the present invention;

fig. 8 is a schematic structural view of the bucket of the present invention.

Description of reference numerals:

1. an analyzer; 2. a heating tank; 3. heating the carrier plate; 4. heating the sleeve; 5. a first flow passage; 6. a second flow passage; 7. a first tube interface; 8. a second tube interface; 9. hanging a bolt; 10. a water bucket; 11. a clamping hole; 12. a temperature sensor; 13. a liquid crystal display screen; 14. a cover plate; 15. a fluorescence detector; 16. a slider; 17. a limiting block; 18. a surrounding baffle frame; 19. a chute; 20. a control panel; 21. a power switch; 22. a power interface; 23. a base plate; 24. support the feet.

Detailed Description

In order to make the technical solutions of the present invention better understood, those skilled in the art will now describe the present invention in further detail with reference to the accompanying drawings.

The present invention provides a quantitative analyzer for PCR polymerase chain reaction as shown in FIGS. 1-8, comprising an analyzer 1, the top of the analyzer 1 is provided with a heating pool 2, the top of the heating pool 2 is clamped with a heating carrier plate 3, the bottom of the heating carrier plate 3 is fixedly connected with a heating sleeve 4, the inner wall of the heating pool 2 is provided with a first flow passage 5 and a second flow passage 6, a first pipe interface 7 and a second pipe interface 8 are fixedly connected with the back of the analyzer 1, one end of the first flow channel 5 is communicated with the first pipe interface 7, the second flow channel 6 is communicated with a second pipe interface 8, the back of the analyzer 1 is fixedly connected with a hanging bolt 9, the hanging bolt 9 is arranged at the bottom of the first pipe connector 7 and the second pipe connector 8, the back of the analyzer 1 is provided with a water bucket 10, a clamping hole 11 is formed in one side of the water bucket 10, and the hanging bolt 9 is hung in the clamping hole 11.

The bottom end of the heating sleeve 4 extends into the heating pool 2, the number of the heating sleeve 4 is set to be a plurality of, a plurality of the heating sleeve 4 is uniformly distributed.

2 inner wall fixedly connected with temperature sensor 12 in heating bath, 1 openly fixedly connected with liquid crystal display 13 of analysis machine, temperature sensor 12 and liquid crystal display 13 all with 1 electric connection of analysis machine.

The top of the analyzer 1 is provided with a fluorescence detection mechanism, the fluorescence detection mechanism comprises a cover plate 14, the cover plate 14 is matched with the heating pool 2, the bottom of the cover plate 14 is fixedly connected with a fluorescence detector 15, and the fluorescence detector 15 is electrically connected with the analyzer 1.

The equal fixedly connected with slider 16 in apron 14 both sides, two the equal fixedly connected with stopper 17 in slider 16 one side, 1 top fixedly connected with of analysis machine encloses and keeps off frame 18, enclose and keep off 18 inner wall both sides of frame and all seted up spout 19, two stopper 17 respectively with two spout 19 sliding connection.

The analytical engine 1 top one end fixedly connected with control panel 20, analytical engine 1 openly fixedly connected with switch 21, control panel 20 and switch 21 all with analytical engine 1 electric connection.

Analyzer 1 one side fixedly connected with power source 22, power source 22 and analyzer 1 electric connection, 1 bottom fixedly connected with bottom plate 23 of analyzer, power source 22 set up in analyzer 1 one side, can avoid power source 22 to take place into water.

The bottom of the bottom plate 23 is fixedly connected with four supporting legs 24, and the number of the supporting legs 24 is four, and the four supporting legs 24 are uniformly distributed at four corners of the bottom plate 23.

This practical theory of operation:

referring to the attached drawings 1-8 of the specification, when the device is used, firstly, a heating pool 2 is connected with an external steam generator through a first pipe interface 7 and a second pipe interface 8, a heating carrier plate 3 is clamped at the top of the heating pool 2, the heating pool 2 is sealed, a reaction test tube is inserted into a heating sleeve 4, then a cover plate 14 is moved to the top of the heating pool 2, a fluorescence detector 15 is started to monitor the reaction process in real time, the steam generator injects steam into the heating pool 2 through the first pipe interface 7 and a first flow passage 5, condensed water generated after steam heat transfer is pumped away by the steam generator through a second flow passage 6 and a second pipe interface 8, so that a steam cycle is formed, the heating sleeve 4 is heated through high-temperature steam, the heating sleeve 4 transfers heat to the reaction test tube, template DNA in the reaction test tube is denatured into a single chain after being heated to about 94 ℃ for a certain time, then, cooling water is injected into the heating pool 2 through a steam generator, the temperature is reduced to about 55 ℃, annealing (renaturation) is carried out on the template DNA and the primers, finally, steam is injected into the heating pool 2 through the steam generator, the temperature is increased to about 72 ℃, the primers are extended, so that a cycle is completed, the cycle is repeated for 20-40 times, each cycle comprises three steps of reactions including high-temperature denaturation, low-temperature annealing and medium-temperature extension, the reaction temperature is monitored through a temperature sensor 12 in the whole process, the reaction temperature is conveniently controlled, a water containing bucket 10 is hung on the back of the analyzer 1, and when the connection between the steam generator and the first pipe connector 7 and the connection between the steam generator and the second pipe connector 8 are disassembled, the water containing bucket 10 can collect residual condensed water;

referring to the attached drawings 1-8 of the specification, when the device is used, a fluorescence detection mechanism is arranged to monitor the reaction process, the cover plate 14 is moved to the top of the heating pool 2, the fluorescence detector 15 at the bottom of the cover plate 14 is just above the heating support plate 3, and meanwhile, the cover plate 14 provides a dark environment for the fluorescence detector 15, so that the fluorescence detector 15 can conveniently work.

Claims

1. A PCR quantitative analyzer comprises an analyzer (1), and is characterized in that: the analyzer comprises an analyzer (1), a heating pool (2) is arranged at the top of the analyzer (1), a heating support plate (3) is clamped at the top of the heating pool (2), a heating sleeve (4) is fixedly connected to the bottom of the heating support plate (3), a first flow channel (5) and a second flow channel (6) are arranged on the inner wall of the heating pool (2), a first pipe connector (7) and a second pipe connector (8) are fixedly connected to the back of the analyzer (1), one end of the first flow channel (5) is communicated with the first pipe connector (7), the second flow channel (6) is communicated with the second pipe connector (8), a hanging bolt (9) is fixedly connected to the back of the analyzer (1), the hanging bolt (9) is arranged at the bottoms of the first pipe connector (7) and the second pipe connector (8), a water containing hopper (10) is arranged on the back of the analyzer (1), a clamping hole (11) is arranged on one side of the water containing hopper (10), the hanging bolt (9) is hung with the clamping hole (11).

2. The quantitative analyzer for PCR according to claim 1, wherein: heating sleeve pipe (4) bottom extends to inside heating bath (2), heating sleeve pipe (4) quantity is established to a plurality ofly, and is a plurality of heating sleeve pipe (4) evenly distributed.

3. The quantitative analyzer for PCR according to claim 1, wherein: heating bath (2) inner wall fixedly connected with temperature sensor (12), the positive fixedly connected with liquid crystal display (13) of analysis machine (1), temperature sensor (12) and liquid crystal display (13) all with analysis machine (1) electric connection.

4. The quantitative analyzer for PCR according to claim 1, wherein: the fluorescence detection device is characterized in that a fluorescence detection mechanism is arranged at the top of the analyzer (1), the fluorescence detection mechanism comprises a cover plate (14), the cover plate (14) is matched with the heating pool (2), a fluorescence detector (15) is fixedly connected to the bottom of the cover plate (14), and the fluorescence detector (15) is electrically connected with the analyzer (1).

5. The quantitative analyzer for PCR according to claim 4, wherein: apron (14) equal fixedly connected with slider (16) in both sides, two equal fixedly connected with stopper (17) in slider (16) one side, analysis machine (1) top fixedly connected with encloses fender frame (18), enclose and keep off frame (18) inner wall both sides and all seted up spout (19), two stopper (17) respectively with two spout (19) sliding connection.

6. The quantitative analyzer for PCR according to claim 1, wherein: one end of the top of the analyzer (1) is fixedly connected with a control panel (20), the front of the analyzer (1) is fixedly connected with a power switch (21), and the control panel (20) and the power switch (21) are both electrically connected with the analyzer (1).

7. The quantitative analyzer for PCR according to claim 1, wherein: the analysis machine (1) one side fixedly connected with power source (22), power source (22) and analysis machine (1) electric connection, analysis machine (1) bottom fixedly connected with bottom plate (23).

8. The quantitative analyzer for PCR according to claim 7, wherein: the bottom of the bottom plate (23) is fixedly connected with supporting legs (24), the number of the supporting legs (24) is four, and the supporting legs (24) are uniformly distributed at four corners of the bottom plate (23).