CN112326606A - In-vitro diagnosis and analysis system, optical detection device and motion disc module - Google Patents
In-vitro diagnosis and analysis system, optical detection device and motion disc module Download PDFInfo
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- CN112326606A CN112326606A CN202010951039.7A CN202010951039A CN112326606A CN 112326606 A CN112326606 A CN 112326606A CN 202010951039 A CN202010951039 A CN 202010951039A CN 112326606 A CN112326606 A CN 112326606A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Abstract
The invention discloses an in vitro diagnosis and analysis system, an optical detection device and a motion disc module, wherein the motion disc module comprises an installation unit and a lens component, the installation unit is provided with a light path channel, the light path channel comprises a first channel, a second channel and a shared channel, the first channel and the second channel are staggered, and one end of the first channel and one end of the second channel are both communicated with one end of the shared channel to form a shared cavity; the lens components correspond to the light path channels one by one, each lens component comprises a dichroic mirror, an excitation sheet and an emission sheet, the dichroic mirrors are arranged in the shared cavity, the excitation sheets are arranged in the first channels and form first light paths with the dichroic mirrors, and the emission sheets are arranged in the second channels and form second light paths with the dichroic mirrors. The optical detection device adopts the motion disc module, so that the winding problem can be avoided. The in-vitro diagnosis and analysis system applies the optical detection device, so that the design is more flexible, and the control is easier.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnosis, in particular to an in-vitro diagnosis and analysis system, an optical detection device and a motion disk module.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify a specific DNA fragment. The Real-time Quantitative polymerase chain reaction (qPCR) is a method of adding a corresponding fluorescent dye or a fluorescent labeled probe based on conventional PCR, Detecting the whole PCR process in Real time through fluorescent signal change during the PCR reaction process, monitoring the total amount of products after each PCR cycle with a fluorescent chemical substance, and quantitatively analyzing a specific DNA sequence in a sample to be detected. The fluorescent quantitative PCR instrument is a reaction instrument for real-time detection by applying qPCR technology, and the functions of the instrument are generally ensured by a thermal cycle system and a fluorescent real-time detection system.
At present, the qPCR technology for in vitro diagnosis usually requires the detection of multiple indicators (multiple target detection objects) in one test for one or more samples. The conventional optical detection device uses a plurality of independent optical units to detect a plurality of target detection objects in the same reaction chamber or to detect the same target object in a plurality of reaction chambers. This leads to complicated winding and over-large overall volume in the optical detection device, which is not favorable for the miniaturization development of the in vitro diagnostic and analysis system.
Disclosure of Invention
In view of the above, there is a need for an in vitro diagnostic and analysis system, an optical detection device and a motion disk module. This motion dish module is independent of detection module and light source module setting, can move alone, so can avoid the wire winding problem. The optical detection device adopts the motion disc module, can independently drive the motion disc module to move, can avoid the winding problem, has more flexible design, and is favorable for the miniaturization development of an in vitro diagnosis and analysis system. The in-vitro diagnosis and analysis system applies the optical detection device, so that the design is more flexible, and the control is easier.
The technical scheme is as follows:
on one hand, the application provides a motion disc module, which comprises an installation unit and a lens assembly, wherein the installation unit is provided with a light path channel, the light path channel comprises a first channel, a second channel and a shared channel, the first channel and the second channel are staggered, and one end of the first channel and one end of the second channel are both communicated with one end of the shared channel to form a shared cavity; the lens components correspond to the light path channels one by one, each lens component comprises a dichroic mirror, an excitation sheet and an emission sheet, the dichroic mirrors are arranged in the shared cavity, the excitation sheets are arranged in the first channels and form first light paths with the dichroic mirrors, and the emission sheets are arranged in the second channels and form second light paths with the dichroic mirrors.
And forming a light path channel comprising a first channel, a second channel and a shared channel by using the mounting unit, and then integrating the lens assembly on the mounting unit, so that the excitation sheet and the dichroic mirror form a first light path, and the emission sheet and the dichroic mirror form a second light path. So, rotatory or remove the installation element and can realize the switching of light path passageway, be applied to optical detection device, light-emitting component and detecting element set up along the movement track interval of installation element for light path passageway optionally docks with light-emitting component and detecting element, realizes the detection to the heterogeneous target detection thing of target sample, and this process light source module and detecting element all need not to rotate, can not have the wire winding problem, consequently need not to set up the wire winding and dodges the space.
The technical solution is further explained below:
in one embodiment, the mounting unit includes a light shield, a first plate and a second plate, the light shield is provided with a light path channel, at least two light shields are clamped between the first plate and the second plate at intervals, the first plate is disposed above the light shield, the first plate is provided with a first through hole communicated with the second channel, and the second plate is provided with a second through hole communicated with the common channel.
In one embodiment, the number of the optical path channels is at least two, and the light shields correspond to the optical path channels one to one.
In one embodiment, the at least two light shields are clamped between the first plate body and the second plate body at intervals along the same circumference to form a group of installation modules, and the installation unit comprises one group or more than two groups of installation modules.
In one embodiment, the mounting unit comprises at least two sets of mounting modules arranged in a vertical stack.
In one embodiment, the lens assembly further comprises a first light folding member disposed in the first channel and disposed between the excitation sheet and the dichroic mirror or between the excitation sheet and the first lens; and/or the lens assembly further comprises a second light folding piece, wherein the second light folding piece is arranged in the second channel and is arranged between the emission piece and the dichroic mirror or between the emission piece and the second lens.
In one embodiment, the moving disk module further comprises a third lens disposed between the dichroic mirror and the sample disk.
In one embodiment, the lens assembly further includes a first lens disposed on the first light path and between the exciting sheet and the light-emitting member; and the second lens is arranged on the second light path and is arranged between the emitting sheet and the detection element.
On the other hand, this application still provides an optical detection device, including foretell motion dish module, still include light source module, detection module and driver, light source module is equipped with two at least luminous pieces, is equipped with first lens between luminous piece and the excitation piece, and detection module is equipped with two at least detecting element, is equipped with the second lens between detecting element and the emission piece, and the driver is used for driving the installation unit and moves.
When the optical detection device is used, the installation unit is utilized to form a light path channel comprising a first channel, a second channel and a shared channel, then the lens assembly is integrated on the installation unit, so that the excitation sheet and the dichroic mirror form a first light path, the emission sheet and the dichroic mirror form a second light path, and the light-emitting piece and the detection element are arranged at intervals along the motion track of the installation unit. So, only need utilize the driver rotatory or remove the installation element and can realize the switching of light path passageway for light path passageway optionally docks with illuminating part and detecting element, realizes the detection to the target detection thing of the different types of target sample, and this process light source module and detecting element all need not to rotate, can not have the wire winding problem, consequently need not to set up the wire winding and dodges the space. The optical detection device adopts the motion disc module, can independently drive the motion disc module to move, can avoid the winding problem, has more flexible design, and is favorable for the miniaturization development of an in vitro diagnosis and analysis system.
The technical solution is further explained below:
in one embodiment, the optical detection device further comprises a first integrated piece, all the first lenses are fixedly arranged on the first integrated piece, and the first integrated piece is fixedly arranged between the mounting unit and the light-emitting piece; or the optical detection device further comprises a second integrated piece, all the second lenses are fixedly arranged on the second integrated piece, and the second integrated piece is fixedly arranged between the mounting unit and the detection element.
In one embodiment, the shared channels of the optical path channels are arranged at intervals along the same circumference, and the driver is used for driving the mounting unit to rotate.
In one embodiment, the optical detection device further comprises a third lens, the third lens being disposed between the dichroic mirror and the sample tray.
In one embodiment, the optical detection apparatus further includes a third integrated member, the third integrated member is fixedly disposed between the mounting unit and the sample tray, and the third lens is fixedly disposed in the third integrated member.
In another aspect, the present application further provides an in vitro diagnostic and analytical system, comprising the optical detection device in any of the above embodiments.
The in vitro diagnosis and analysis system applies the optical detection device, can independently drive the motion disc module to move, can avoid the winding problem, does not need to set an avoiding space, is more flexible in design and is easier to control.
Drawings
FIG. 1 is a schematic diagram of an optical inspection apparatus according to an embodiment;
FIG. 2 is a schematic diagram of an optical inspection apparatus according to an embodiment;
FIG. 3 is a schematic view of the optical path shown in FIG. 2;
FIG. 4 is an exploded view of a portion of the optical inspection device shown in FIG. 2;
FIG. 5 is a schematic structural view of the kinematic disk module shown in FIG. 4;
FIG. 6 is an exploded view of a portion of the kinematic disk module of FIG. 5;
FIG. 7 is a schematic diagram of a PCR chip to be detected.
Description of reference numerals:
10. a motion plate module; 100. a mounting unit; 110. an optical path channel; 112. a first channel; 114. a second channel; 116. a common channel; 118. a common chamber; 120. a light shield; 130. a first plate body; 132. a first through hole; 140. a second plate body; 142. a second through hole; 200. a lens assembly; 210. a dichroic mirror; 220. an excitation sheet; 230. a transmitting sheet; 240. a first lens; 250. a second lens; 260. a third lens; 30. a light source module; 32. a light emitting member; 40. a detection module; 42. a detection element; 50. a second integrated piece; 60. a third integrated piece; 70. a sample tray; 72. a sample chamber.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and the detailed description. It should be understood that the detailed description and specific examples, while indicating the scope of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
It will be understood that when an element is referred to as being "secured to," "disposed on," "secured to," or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. Further, when one element is considered as "fixed transmission connection" with another element, the two elements may be fixed in a detachable connection manner or in an undetachable connection manner, and power transmission can be achieved, such as sleeving, clamping, integrally-formed fixing, welding and the like, which can be achieved in the prior art, and is not cumbersome. When an element is perpendicular or nearly perpendicular to another element, it is desirable that the two elements are perpendicular, but some vertical error may exist due to manufacturing and assembly effects. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
References to "first" and "second" in this disclosure do not denote any particular order or quantity, but rather are used to distinguish one element from another.
At present, the qPCR technology for in vitro diagnosis usually requires the detection of multiple indicators (multiple target detection objects) in one test for one or more samples. The conventional optical detection device uses a plurality of independent optical units to detect a plurality of target detection objects in the same reaction chamber or to detect the same target object in a plurality of reaction chambers.
In the conventional optical detection device, a plurality of independent optical units are adopted, and a rotary or translational mode is required in the detection process, so that the relative position of the optical units or the PCR chamber is changed to realize multiple detections. In addition, a resetting step is often required after each rotation or translation operation, so that the problem of winding or repeated detection is solved, the control is complex, the steps are complicated, and the detection time is too long.
In addition, if the optical system and the reaction chambers are to be kept relatively stationary, the number of optical detection modules corresponding to the number of reaction chambers is required, and each optical module is required to be capable of detecting a plurality of different types of target detection objects simultaneously, which may result in an excessively large detection module. Furthermore, the design and layout of the detection chambers on the cartridge are subject to space constraints of the optical system as well, resulting in a complex optical design.
Therefore, it is necessary to provide an optical inspection apparatus which can avoid the winding problem and has a more flexible design.
As shown in fig. 1 to 2, in one embodiment, an optical detection apparatus is provided, which includes a motion disk module, a light source module 30, a detection module 40, and a driver, which are disposed separately from a detection module 40 and a light source module 30 and can move independently.
As shown in fig. 3 to 6, the moving plate module includes a mounting unit 100 and a lens assembly 200, the mounting unit 100 is provided with an optical path channel 110, the optical path channel 110 includes a first channel 112, a second channel 114 and a common channel 116, the first channel 112 and the second channel 114 are staggered, and one end of the first channel 112 and one end of the second channel 114 are both communicated with one end of the common channel 116 to form a common cavity 118; the optical lens assemblies 200 correspond to the optical path channels 110 one to one, each optical lens assembly 200 includes a dichroic mirror 210, a pumping sheet 220 and an emission sheet 230, the dichroic mirror 210 is disposed in the common cavity 118, the pumping sheet 220 is disposed in the first channel 112 and forms a first optical path with the dichroic mirror 210, and the emission sheet 230 is disposed in the second channel 114 and forms a second optical path with the dichroic mirror 210.
As shown in fig. 1 and 2, the light source module 30 has at least two light emitting elements 32, a first lens 240 is disposed between the light emitting elements 32 and the exciting sheet 220, the detection module 40 has at least two detection elements 42, a second lens 250 is disposed between the detection elements 42 and the emitting sheet 230, and the driver is used for driving the mounting unit 100 to move.
In use, the optical detection device is assembled with the mounting unit 100 to form the optical path channel 110 including the first channel 112, the second channel 114 and the common channel, and then the lens assembly 200 is integrated with the mounting unit 100, such that the excitation sheet 220 and the dichroic mirror 210 form a first optical path, the emission sheet 230 and the dichroic mirror 210 form a second optical path, and the light-emitting element 32 and the detection element 42 are spaced along the movement track of the mounting unit 100. Thus, the switching of the optical path 110 can be realized only by rotating or moving the mounting unit 100 by the driver, so that the optical path 110 can be selectively butted with the light emitting element 32 and the detecting element 42 to detect different types of target objects of the target sample, and the light source module 30 and the detecting element 42 do not need to be rotated in the process, so that the winding problem does not exist, and a winding avoiding space does not need to be arranged. The optical detection device adopts the motion disc module, can drive the motion disc module to move independently, or drive the motion disc module and the light source module to move together, or drive the motion disc module and the detection module to move together, can avoid the winding problem, has more flexible design, and is favorable for the miniaturization development of an in vitro diagnosis and analysis system.
Specifically, as shown in fig. 3, when the optical detection device is applied to in vitro diagnostic analysis, light emitted from the light-emitting element 32 passes through the first lens 240 and the excitation sheet 220 and then emits to the dichroic mirror 210, the light is reflected by the dichroic mirror 210 and then emits to the sample cavity 72 (in this process, the third lens 260 may be used for focusing), a sample in the sample cavity 72 is excited by the light from the light-emitting element 32 and emits fluorescence, the fluorescence emits to the dichroic mirror 210, the fluorescence emits to the emission sheet 230 through the dichroic mirror 210, the fluorescence passes through the emission sheet 230 and then emits to the second lens 250, and the fluorescence is focused on the detection element 42 by the second lens 250. After the detection of one type of object is completed, the driver drives the mounting unit 100 to rotate, so that the optical path 110 corresponds to the other light-emitting element 32 and the other detecting element 42, and the above operation is continued to complete the detection of the other type of object.
It is to be noted that in the context of the present invention, any kind of device capable of emitting a monochromatic or broadband electromagnetic field will be understood as being encompassed by the term "light emitter 32". Further, an array of multiple light emitters 32 having equal or different characteristics with respect to frequency, polarization, flux, electrical input power, or technology for emitting photons is also encompassed within the term "light emitter 32". For example, Light Emitting Diodes (LEDs), Organic Light Emitting Diodes (OLEDs), Polymer Light Emitting Diodes (PLEDs), quantum dot based emitters 32, white emitters 32, halogen lamps, lasers, solid state lasers, laser diodes, micro wire lasers, diode solid state lasers, vertical cavity surface emitting lasers, phosphor coated LEDs, thin film electroluminescent devices, phosphorescent OLEDs, inorganic/organic LEDs, LEDs using quantum dot technology, LED arrays, flood systems using LEDs, white LEDs, incandescent lamps, arc lamps, gas lamps, and fluorescent tubes, are intended to be encompassed by the term "emitter 32".
"detection element 42" in the context of the present invention includes any device capable of detecting electromagnetic radiation within the term "detection element 42". Such as Charge Coupled Devices (CCDs), photodiodes, photodiode arrays. Furthermore, the detection element 42 can be adapted in such a way that the detected radiation and the correspondingly generated information can be fed to a memory, a computer or another control unit.
"driver" in the context of the present invention may be selected according to the desired motion trajectory of the mounting unit 100, including robotic arms, telescopic devices, reciprocating devices, swing drive devices, etc., as well as devices that directly provide rotational power, including servo motors, rotary hydraulic cylinders, etc., as well as other devices that indirectly provide power. The above can be realized in the prior art, and the details are not repeated herein.
"sample" in the context of the present invention, the term "sample" as used will refer to any kind of substance, including one or several components detected by optical detection, e.g. by optical excitation and subsequent optical reading. For example, biochemical substances may be analyzed in the context of the present invention. In addition, the sample may be a substance used in the fields of molecular diagnostics, clinical diagnostics, gene and protein expression arrays. The components of the sample (the components to be detected) may in particular be any substance which can be copied by PCR.
It should be noted that, the phrase "the first lens 240 is disposed between the light emitting element 32 and the exciting sheet 220" includes that the first lens 240 is fixedly disposed in the light source module 30 and is combined with the corresponding light emitting element 32, and at this time, the first lens 240 does not rotate with the mounting unit 100; or the first lens 240 is fixed between the corresponding light emitting element 32 and the exciting sheet 220 by using other mounting structures, and at this time, the first lens 240 does not rotate along with the mounting unit 100; or the first lens 240 is directly or indirectly fixed to the mounting unit 100 to rotate with the mounting unit 100.
Optionally, in an embodiment, the optical detection apparatus further includes a first integrated member (not shown), and all the first lenses 240 are fixed on the first integrated member, and the first integrated member is fixed between the mounting unit 100 and the detection element 42. At this time, the first lens 240 is provided at a sidewall of the mounting unit 100. In this manner, the integration of the first lens 240 into the moving disc module is achieved using the first integration member in combination with the mounting unit 100.
"a second lens 250 is disposed between the detecting element 42 and the emitting sheet 230" includes that the second lens 250 is fixedly disposed in the detecting module 40 and is combined with the corresponding detecting element 42, and at this time, the second lens 250 does not rotate along with the mounting unit 100; or the second lens 250 is fixedly arranged between the corresponding detecting element 42 and the emitting sheet 230 by using other mounting structures, and at this time, the second lens 250 does not rotate along with the mounting unit 100; or the second lens 250 is directly or indirectly fixed to the mounting unit 100 to rotate with the mounting unit 100.
Specifically, in the present embodiment, as shown in fig. 1, the optical detection apparatus further includes a second integrated member 50, all the second lenses 250 are fixedly disposed on the second integrated member 50, and the second integrated member 50 is fixedly disposed between the mounting unit 100 and the detection element 42. In this manner, the integration of the second lens 250 into the moving disk module is achieved using the second integration piece 50 in combination with the mounting unit 100.
Of course, in other embodiments, the lens assembly 200 further includes a first lens 240, and the first lens 240 is disposed on the first optical path and disposed between the exciting sheet 220 and the light-emitting element 32. That is, the first lens 240 may be directly integrated to the mounting unit 100.
Or the lens assembly 200 further includes a second lens 250, and the second lens 250 is disposed on the second optical path and disposed between the emitting chip 230 and the detecting element 42. That is, the second lens 250 may be directly integrated to the mounting unit 100.
The lens assembly 200 further includes a first lens element 240, wherein the first lens element 240 is disposed on the first optical path and disposed between the exciting sheet 220 and the light emitting element 32; the lens assembly 200 further includes a second lens 250, and the second lens 250 is disposed on the second optical path and between the emitting chip 230 and the detecting element 42. That is, the first lens 240 and the second lens 250 may be directly integrated onto the mounting unit 100.
On the basis of any of the above embodiments, as shown in fig. 1 to 5, in one embodiment, there is one optical path channel 110.
Alternatively, the number of the optical path channels 110 is two or more. In this way, the optical path channels 110 can correspond to the detection modules 40 and the light source modules 30 one by one, so that different types of target detection objects of different samples can be detected, and at least two types of target detection objects of at least two different samples can be detected at a time.
The "mounting unit 100" may be any mounting structure capable of mounting the above-described components, such as a mounting bracket, a mounting seat, and a mounting case.
Further, as shown in fig. 5 and 6, in an embodiment, the mounting unit 100 includes light-shielding covers 120, a first plate 130 and a second plate 140 corresponding to the light-path channels 110 one by one, the light-shielding covers 120 are provided with one light-path channel 110, at least two light-shielding covers 120 are sandwiched between the first plate 130 and the second plate 140 at intervals, the first plate 130 is disposed above the light-shielding covers 120, the first plate 130 is provided with a first through hole 132 communicating with the second channel 114, and the second plate 140 is provided with a second through hole 144 communicating with the common channel 116. Thus, the lens assembly 200 can be conveniently mounted and conveniently designed and combined by combining the light shield 120 with the first plate 130 and the second plate 140.
At least two light path channels 110 can be realized by using at least two light shields, so that the lens assembly 200 is convenient to mount and design and combine. Meanwhile, the optical path channel 110 is disposed in the light shield 120, so that the detection precision can be prevented from being affected by light pollution.
Further, as shown in fig. 5 and 6, in an embodiment, at least two light shields 120 are sandwiched between the first plate 130 and the second plate 140 at intervals along the same circumference to form a set of mounting modules, and the mounting unit 100 includes one or more than two sets of mounting modules. Thus, the installation module can be formed, and the modular assembly is convenient.
Further, the mounting unit 100 includes more than two sets of mounting modules arranged in a vertically stacked manner. So, can make full use of optical detection device's vertical space, can vertical stack set up more than two sets of installation module, and each other do not influence, be favorable to integrating more light source module 30 and detection module 40, be convenient for improve detection efficiency. Meanwhile, the modular assembly can be carried out, which is beneficial to reducing the manufacturing cost.
Of course, in other embodiments, other means may be utilized to form the desired structure on the mounting unit 100.
In addition to any of the above embodiments, in an embodiment, the lens assembly 200 further includes a first light-folding member disposed in the first channel 112 and disposed between the excitation sheet 220 and the dichroic mirror 210 or disposed between the excitation sheet 220 and the first lens 240; or the lens assembly 200 further includes a second light-folding element disposed in the second channel 114 and disposed between the emission sheet 230 and the dichroic mirror 210 or between the emission sheet 230 and the second lens 250; the lens assembly 200 further includes a first light-folding member disposed in the first channel 112 and disposed between the excitation sheet 220 and the dichroic mirror 210 or between the excitation sheet 220 and the first lens 240; the lens assembly 200 further includes a second light-folding member disposed in the second channel 114 and disposed between the emission sheet 230 and the dichroic mirror 210 or between the emission sheet 230 and the second lens 250. Therefore, the change of the light path is realized by the first light folding part or/and the second light folding part, so that the detection module 40 and the light source module 30 are more flexibly arranged, and the internal space of the in vitro diagnosis and analysis system can be flexibly arranged.
It should be noted that the "first light-folding member" and the "second light-folding member" include, but are not limited to, any prior art implementation that can implement a light path change, such as a mirror, a prism, and an optical fiber. .
In addition to any of the above embodiments, as shown in fig. 3, in an embodiment, the lens assembly 200 further includes a third lens 260, and the third lens 260 is disposed in the common channel 116 and below the dichroic mirror 210. In this manner, the third lens 260 may be directly integrated into the mounting unit 100, moving synchronously with the mounting unit 100.
Alternatively, as shown in fig. 1, in an embodiment, the optical detection apparatus further includes a third integrated member 60, the third lens 260 is fixedly disposed in the third integrated member 60, and the third integrated member 60 is fixedly disposed between the mounting unit 100 and the sample tray 70. In this way, the third lens 260 can be modularly assembled by using the third integration member 60 and then integrated into the mounting unit 100 to be moved synchronously with the mounting unit 100.
Of course, in other embodiments, the third lens 260 may be integrated into the sample tray; or other mounting structure.
In addition to any of the above embodiments, as shown in fig. 2 and 3, in an embodiment, the common channels of the optical path channels 110 are arranged at intervals along the same circumference, and the driver is used for driving the mounting unit 100 to rotate. Therefore, detection of different target detection objects can be realized only by rotation, and the detection device is simple to control and easy to realize. Meanwhile, the at least two light shields 120 are clamped between the first plate 130 and the second plate 140 at intervals along the same circumference to form a set of mounting modules, and the mounting unit 100 includes more than two sets of mounting modules vertically stacked. Thus, more elements can be integrated with rotation, enabling simultaneous detection of more sample chambers 72.
On the other hand, the present application further provides an in vitro diagnostic and analysis system, which includes the optical detection device in any of the above embodiments, and further includes a sample tray 70, the sample tray 70 is provided with detection cavities 72 corresponding to the free ends of the common channels one by one, and a third lens 260 is provided between the detection cavities 72 and the common channels.
The in vitro diagnosis and analysis system applies the optical detection device, can independently drive the motion disc module to move, can avoid the winding problem, does not need to set an avoiding space, is more flexible in design and is easier to control.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (14)
1. A sports disc module, comprising:
the mounting unit is provided with a light path channel, the light path channel comprises a first channel, a second channel and a shared channel, the first channel and the second channel are staggered, one end of the first channel and one end of the second channel are both communicated with one end of the shared channel, and a shared cavity is formed; and
the lens assembly is installed on the installation unit and corresponds to the light path channels one by one, the lens assembly comprises a dichroic mirror, an excitation sheet and an emission sheet, the dichroic mirror is arranged in the shared cavity, the excitation sheet is arranged in the first channel and forms a first light path with the dichroic mirror, and the emission sheet is arranged in the second channel and forms a second light path with the dichroic mirror.
2. The sports disc module according to claim 1, wherein the mounting unit includes a light shield, a first plate and a second plate, the light shield is provided with one of the light path passages, the light shield is sandwiched between the first plate and the second plate at an interval, the first plate is disposed above the light shield, the first plate is provided with a first through hole communicating with the second passage, and the second plate is provided with a second through hole communicating with the common passage.
3. The sport disc module of claim 2 wherein said light path channels are at least two, said light shields corresponding one-to-one with said light path channels.
4. The sports disc module according to claim 3, wherein at least two light shields are sandwiched between the first plate and the second plate at intervals along the same circumference to form a set of mounting modules, and the mounting unit includes one or more than two sets of the mounting modules.
5. A sports disc module according to claim 4, wherein the mounting unit includes more than two sets of mounting modules arranged in vertical stacks.
6. The moving disk module of any of claims 1-5, further comprising a first lens disposed on the first optical path and between the excitation sheet and the glowing member; and/or the moving disk module further comprises a second lens, wherein the second lens is arranged on the second light path and is arranged between the emitting sheet and the detection element.
7. The moving plate module of claim 6, further comprising a third lens disposed between the dichroic mirror and the sample plate.
8. The sport disc module of claim 6 wherein lens assembly further comprises a first light folding member disposed within said first channel and disposed between said excitation sheet and said dichroic mirror or between said excitation sheet and said first lens; and/or the lens assembly further comprises a second light folding piece, wherein the second light folding piece is arranged in the second channel and is arranged between the emission piece and the dichroic mirror or between the emission piece and the second lens.
9. An optical inspection apparatus comprising the moving disk module according to any one of claims 1 to 8, further comprising:
the light source module is provided with at least two light-emitting pieces, and a first lens is arranged between the light-emitting pieces and the exciting sheet;
the detection module is provided with at least two detection elements, and a second lens is arranged between the detection elements and the emission sheet; and
a driver for driving the mounting unit to move.
10. The optical inspection device of claim 9, further comprising a first integration member, all of the first lenses being fixed to the first integration member; or the optical detection device further comprises a second integrated piece, and all the second lenses are fixedly arranged on the second integrated piece.
11. The optical inspection device of claim 9, wherein the common channels of the optical path channels are spaced along a same circumference, and the driver is configured to drive the mounting unit to rotate.
12. The optical detection device according to any one of claims 9 to 11, characterized in that the optical detection device further comprises a third lens disposed between the dichroic mirror and the sample tray.
13. The optical detection device of claim 12, further comprising a third integrated member, the third integrated member being fixedly disposed between the mounting unit and the sample tray, and the third lens being fixedly disposed within the third integrated member.
14. An in vitro diagnostic assay system comprising an optical detection device according to any one of claims 9 to 13.
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| PCT/CN2020/138006 WO2022052362A1 (en) | 2020-09-11 | 2020-12-21 | In vitro diagnostic analysis system, optical detection apparatus, and motion disc module |
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| WO2022052362A1 (en) | 2022-03-17 |
| CN112326606B (en) | 2022-05-10 |
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