CN112161927A - In-vitro diagnosis and analysis system and optical detection device - Google Patents
In-vitro diagnosis and analysis system and optical detection device Download PDFInfo
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- CN112161927A CN112161927A CN202010951053.7A CN202010951053A CN112161927A CN 112161927 A CN112161927 A CN 112161927A CN 202010951053 A CN202010951053 A CN 202010951053A CN 112161927 A CN112161927 A CN 112161927A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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Abstract
The invention discloses an in vitro diagnosis and analysis system and an optical detection device, wherein the optical detection device comprises a lens module, a light source module, a detection module and a driver, the lens module comprises a first mounting unit, a dichroic mirror, an excitation sheet and an emission sheet, the dichroic mirror and the excitation sheet are arranged in the first mounting unit at intervals and matched with the excitation sheet to form an excitation light path, and the dichroic mirror and the emission sheet are arranged in the first mounting unit at intervals and matched with the emission sheet to form an emission light path; the light source module comprises a second mounting unit and a light-emitting piece, the light-emitting piece is arranged on the second mounting unit, and the light-emitting piece and the exciting sheet are arranged at intervals; the detection module comprises a third installation unit and a detection element, the detection element is arranged on the third installation unit, and the detection element and the emission sheet are arranged at intervals; the driver is used for driving the first mounting unit to move. The optical detection device is more compact in layout. The in-vitro diagnosis and analysis system applies the optical detection device, and is beneficial to reducing the volume.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnosis, in particular to an in-vitro diagnosis analysis system and an optical detection device.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify a specific DNA fragment. The Real-time Quantitative polymerase chain reaction (qPCR) is a method of adding a corresponding fluorescent dye or a fluorescent labeled probe based on conventional PCR, Detecting the whole PCR process in Real time through fluorescent signal change during the PCR reaction process, monitoring the total amount of products after each PCR cycle with a fluorescent chemical substance, and quantitatively analyzing a specific DNA sequence in a sample to be detected. The fluorescent quantitative PCR instrument is a reaction instrument for real-time detection by applying qPCR technology, and the functions of the instrument are generally ensured by a thermal cycle system and a fluorescent real-time detection system.
At present, the qPCR technology for in vitro diagnosis usually requires the detection of multiple indicators (multiple target detection objects) in one test for one or more samples. The conventional optical detection device utilizes a plurality of independent optical units to detect a plurality of target detection objects in the same reaction chamber or to detect the same or different target detection objects in a plurality of reaction chambers. During the detection process, the relative positions of the optical unit and the PCR chamber are changed in a rotating or translating manner to complete the detection. A reset step is often added after each rotation or translation operation to solve the winding problem. This results in a complicated detection process, complicated steps, long detection time and an excessively large overall size of the optical detection device, which is not conducive to the miniaturization development of in vitro diagnostic and analysis systems.
Disclosure of Invention
In view of the above, there is a need for an in vitro diagnostic and analytical system and an optical detection device. The optical detection device does not have the independent optical unit mentioned above, but comprises the relatively independent lens module, the light source module and the detection module, and each module can independently move, so that the winding problem can be avoided, the layout is more compact, the detection time is shortened, and the miniaturization development requirement of the in vitro diagnosis and analysis system can be adapted to.
The technical scheme is as follows:
on one hand, the application provides an optical detection device, which comprises a lens module, a light source module, a detection module and a driver, wherein the lens module comprises a first mounting unit, a dichroic mirror, an excitation sheet and an emission sheet, the dichroic mirror and the excitation sheet are arranged in the first mounting unit at intervals and matched with the excitation sheet to form an excitation light path, and the dichroic mirror and the emission sheet are arranged in the first mounting unit at intervals and matched with the emission sheet to form an emission light path; the light source module comprises a second mounting unit and at least two light-emitting pieces, wherein the light-emitting pieces are arranged on the second mounting unit at intervals, and the light-emitting pieces and the exciting sheet are arranged at intervals; the detection module comprises a third installation unit and at least two detection elements, wherein the detection elements are arranged on the third installation unit at intervals, and the detection elements and the emission sheet are arranged at intervals; the driver is used for driving the first mounting unit, and is used for driving the first mounting unit and the second mounting unit or is used for driving at least one of the first mounting unit and the third mounting unit to move.
Utilize relatively independent lens module, light source module and detection module to mutually support and assemble into optical detection device, this in-process, each structure modularization design and production equipment, then carry out the modularization equipment for circuit layout is more reasonable between the optical detection device, and is compacter between the module. When the optical detection device is used, the excitation light path and the emission light path can be selectively butted with the luminous piece and the detection element only by utilizing the driver to rotate or move the first mounting unit, the first mounting unit and the second mounting unit or at least one of the first mounting unit and the third mounting unit, so that different target detection objects of a target sample can be detected, the light source module and the detection element do not need to rotate in the process, the winding problem does not exist, the winding avoiding space does not need to be arranged, and the volume of the optical detection device can be smaller. This optical detection device passes through the modularized design for the overall arrangement of inside module is compacter, can drive the lens module alone and move, can avoid the wire winding problem, and the design is more nimble, can adapt to external diagnostic analysis system's miniaturized development needs.
The technical solution is further explained below:
in one embodiment, the light-emitting members are arranged at intervals along the same circumference, the detecting elements are arranged at intervals along the other circumference, and the driver is used for driving the first mounting unit to rotate along the center of the same circumference; or the light-emitting pieces are arranged at intervals along a straight line, the detection elements are arranged at intervals along another straight line, and the driver is used for driving the first mounting unit to reciprocate along the straight line.
In one embodiment, the optical detection device further comprises a first lens, wherein the first lens is arranged between the exciting sheet and the light-emitting piece; and/or the optical detection device further comprises a second lens, and the second lens is arranged between the emission sheet and the detection element.
In one embodiment, the first mounting unit is provided with an optical path channel, the optical path channel comprises a first channel, a second channel and a shared channel, the first channel and the second channel are staggered, and one end of the first channel and one end of the second channel are both communicated with one end of the shared channel to form a shared cavity; the lens module corresponds to the light path channels one by one, the dichroic mirror is arranged in the shared cavity, the excitation sheet is arranged in the first channel and forms an excitation light path with the dichroic mirror, and the emission sheet is arranged in the second channel and forms an emission light path with the dichroic mirror.
In one embodiment, the first mounting unit includes a light shield, a first plate and a second plate, the light shield is provided with a light path channel, at least two light shields are clamped between the first plate and the second plate at intervals, the first plate is arranged above the light shield, the first plate is provided with a first through hole communicated with the second channel, and the second plate is provided with a second through hole communicated with the common channel.
In one embodiment, the number of the optical path channels is at least two, and the light shields correspond to the optical path channels one to one.
In one embodiment, the at least two light shields are clamped between the first plate body and the second plate body at intervals along the same circumference to form a group of installation modules, and the first installation unit comprises one group or more than two groups of installation modules.
In one embodiment, the first mounting unit comprises at least two sets of mounting modules arranged in a vertical stack.
In one embodiment, the lens assembly further includes a first light-folding member disposed in the first channel and disposed between the excitation sheet and the dichroic mirror or between the excitation sheet and the first lens; or/and the lens assembly further comprises a second light folding piece, wherein the second light folding piece is arranged in the second channel and is arranged between the emission piece and the dichroic mirror or between the emission piece and the second lens.
In one embodiment, the optical detection device further comprises a third lens, the third lens being disposed between the dichroic mirror and the sample tray.
In one embodiment, the optical detection device further comprises an integrated piece, the third lens is fixedly arranged in the integrated piece, and the integrated piece is fixedly arranged between the first mounting unit and the sample disc.
In another aspect, the present application further provides an in vitro diagnostic and analytical system, comprising the optical detection device in any of the above embodiments.
The in-vitro diagnosis and analysis system applies the optical detection device, can independently drive the lens module, the light source module or the detection module to move, has a more compact structure, and is beneficial to reducing the volume.
Drawings
FIG. 1 is a schematic diagram of an optical inspection apparatus according to an embodiment;
FIG. 2 is a schematic diagram of an optical inspection apparatus according to an embodiment;
FIG. 3 is a schematic view of the optical path shown in FIG. 2;
FIG. 4 is an exploded view of a portion of the optical inspection device shown in FIG. 2;
FIG. 5 is a schematic structural view of the lens module shown in FIG. 4;
FIG. 6 is an exploded view of a portion of the lens module shown in FIG. 5;
FIG. 7 is a schematic diagram of a PCR chip to be detected.
Description of reference numerals:
10. a lens module; 100. a first mounting unit; 110. an optical path channel; 112. a first channel; 114. a second channel; 116. a common channel; 118. a common chamber; 120. a light shield; 130. a first plate body; 132. a first through hole; 140. a second plate body; 142. a second through hole; 200. a dichroic mirror; 300. an excitation sheet; 400. a transmitting sheet; 20. a light source module; 22. a second mounting unit; 24. a light emitting member; 26. a first lens; 30. a detection module; 32. a third mounting unit; 34. a detection element; 36. a second lens; 40. an integrated piece; 42. a third lens; 50. a sample tray; 52. a sample chamber.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and the detailed description. It should be understood that the detailed description and specific examples, while indicating the scope of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
It will be understood that when an element is referred to as being "secured to," "disposed on," "secured to," or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. Further, when one element is considered as "fixed transmission connection" with another element, the two elements may be fixed in a detachable connection manner or in an undetachable connection manner, and power transmission can be achieved, such as sleeving, clamping, integrally-formed fixing, welding and the like, which can be achieved in the prior art, and is not cumbersome. When an element is perpendicular or nearly perpendicular to another element, it is desirable that the two elements are perpendicular, but some vertical error may exist due to manufacturing and assembly effects. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
References to "first" and "second" in this disclosure do not denote any particular order or quantity, but rather are used to distinguish one element from another.
At present, the qPCR technology for in vitro diagnosis usually requires the detection of multiple indicators (multiple target detection objects) in one test for one or more samples. The conventional optical detection device uses a plurality of independent optical units to detect a plurality of target detection objects in the same reaction chamber or to detect the same target object in a plurality of reaction chambers.
In the conventional optical detection device, a plurality of independent optical units are adopted, and a rotary or translational mode is required in the detection process, so that the relative position of the optical units or the PCR chamber is changed to realize multiple detections. In addition, a resetting step is often required after each rotation or translation operation, so that the problem of winding or repeated detection is solved, the control is complex, the steps are complicated, and the detection time is too long.
In addition, if the optical system and the reaction chambers are to be kept relatively stationary, the number of optical detection modules corresponding to the number of reaction chambers is required, and each optical module is required to be capable of detecting a plurality of different types of target detection objects simultaneously, which may result in an excessively large detection module. Furthermore, the design and layout of the detection chambers on the cartridge are subject to space constraints of the optical system as well, resulting in a complex optical design.
In view of the above, there is a need for an optical detection device with a more compact layout that can meet the miniaturization development requirements of in vitro diagnostic and analysis systems.
As shown in fig. 1 to 2, in one embodiment, an optical detection apparatus is provided, which includes a detection module 30 and a light source module 20, which are independently disposed, a lens module 10 capable of moving independently, and a driver (not shown) for driving the lens module 10 to move.
As shown in fig. 2 and 3, the lens module 10 includes a first mounting unit 100, a dichroic mirror 200, an excitation sheet 300, and an emission sheet 400, wherein the dichroic mirror 200 and the excitation sheet 300 are disposed at an interval on the first mounting unit 100 and are matched with the excitation sheet 300 to form an excitation light path, and the dichroic mirror 200 and the emission sheet 400 are disposed at an interval on the first mounting unit 100 and are matched with the emission sheet 400 to form an emission light path; the light source module 20 includes a second mounting unit 22 and at least two light emitting members 24, wherein the light emitting members 24 are spaced apart from each other on the second mounting unit 22, and the light emitting members 24 are spaced apart from the excitation sheet 300; the detection module 30 comprises a third mounting unit 32 and at least two detection elements 34, wherein the detection elements 34 are arranged on the third mounting unit 32 at intervals, and the detection elements 34 and the emission sheet 400 are arranged at intervals; the driver is used for driving at least one of the first mounting unit 100, the first mounting unit 100 and the second mounting unit 22, or the first mounting unit 100 and the third mounting unit 32 to move.
As shown in fig. 2 and 3, the optical detection device is assembled by mutually matching the relatively independent lens module 10, the light source module 20 and the detection module 30, and in the process, each structure is modularly designed, produced and assembled, and then the modularized assembly is performed, so that the circuit layout between the optical detection devices is more reasonable, and the modules are more compact. When the device is used, at least one of the first mounting unit 100, the first mounting unit 100 and the second mounting unit 22 or the first mounting unit 100 and the third mounting unit 32 is driven to rotate or move by using the driver, so that the excitation light path and the emission light path can be selectively butted with the luminous piece 24 and the detection element 34, and different types of target detection objects of the target sample can be detected. The optical detection device has the advantages that through the modular design, the layout of the internal modules is more compact, the lens module 10, the light source module 20 or the detection module 30 can be independently driven to move, and the miniaturization development requirement of the in-vitro diagnosis and analysis system can be met.
Specifically, with the present device, the second mounting unit 22 can be fixed, i.e. the light source module 20 is fixed, and the other components are integrated to rotate, i.e. the lens module 10 and the detection module 30 move together with the driver, so as to realize the related detection. In this process, the light source module 20 is not moved, so that the problem of winding can be reduced.
Or by using the device, the third mounting unit 32 can be fixed, i.e. the detection module 30 is fixed, and the other components are integrated to rotate, i.e. the lens module 10 and the light source module 20 move together with the driver, so as to realize the relevant detection. In the process, the detection module 30 is not moved, so that the problem of winding can be reduced;
or by using the device, the second mounting unit 22 and the third mounting unit 32 can be fixed, that is, the light source module 20 and the detection module 30 are both fixed, and the other components are integrated together to rotate, that is, the lens module 10 moves along with the driving, so as to realize the related detection. In this process, the light source module 20 and the detection module 30 are not moved, i.e. there is no winding problem.
It is noted that in the context of the present invention, any kind of device capable of emitting a monochromatic or broadband electromagnetic field will be understood as being encompassed by the term "light emitter 24". Further, an array of multiple light emitters 24 having equal or different characteristics with respect to frequency, polarization, flux, electrical input power, or technology for emitting photons is also encompassed within the term "light emitter 24". For example, Light Emitting Diodes (LEDs), Organic Light Emitting Diodes (OLEDs), Polymer Light Emitting Diodes (PLEDs), quantum dot based emitters 24, white emitters 24, halogen lamps, lasers, solid state lasers, laser diodes, micro wire lasers, diode solid state lasers, vertical cavity surface emitting lasers, phosphor coated LEDs, thin film electroluminescent devices, phosphorescent OLEDs, inorganic/organic LEDs, LEDs using quantum dot technology, LED arrays, flood systems using LEDs, white LEDs, incandescent lamps, arc lamps, gas lamps, and fluorescent tubes, are intended to be encompassed by the term "emitter 24".
"detection element 34" in the context of the present invention includes any device capable of detecting electromagnetic radiation within the term "detection element 34". Such as Charge Coupled Devices (CCDs), photodiodes, photodiode arrays. Furthermore, the detection element 34 may be adapted in such a way that the detected radiation and the correspondingly generated information may be conveyed to a memory, a computer or another control unit.
"driver" in the context of the present invention may be selected according to the desired motion trajectory of the first mounting unit 100, including robotic arms, telescopic devices, reciprocating devices, swing drive devices, etc., as well as devices that directly provide rotational power, including servo motors, rotary hydraulic cylinders, etc., as well as other devices that indirectly provide power. The above can be realized in the prior art, and the details are not repeated herein.
"sample" in the context of the present invention, the term "sample" as used will refer to any kind of substance, including one or several components detected by optical detection, e.g. by optical excitation and subsequent optical reading. For example, biochemical substances may be analyzed in the context of the present invention. In addition, the sample may be a substance used in the fields of molecular diagnostics, clinical diagnostics, gene and protein expression arrays. The components of the sample (the components to be detected) may in particular be any substance which can be copied by PCR.
In addition to any of the above embodiments, in an embodiment, the optical detection apparatus further includes a first lens 26, and the first lens 26 is disposed between the excitation sheet 300 and the light-emitting element 24. Thus, when the optical detection device is applied to in vitro diagnosis and analysis, light emitted by the light-emitting element 24 is collected by the first lens 26, then emitted to the excitation sheet 300, and can be collimated to incident light, reflected by the dichroic mirror 200, and focused on the sample cavity 52 by the third lens 42
Optionally, the first lens 26 is fixedly disposed on the second mounting unit 22, and the first lens 26 is disposed between the exciting sheet 300 and the light emitting member 24. In this way, the integration of the first lens 26 into the light emitting module is achieved with the second mounting unit 22.
Or in another embodiment, the optical detection device further comprises a second lens 36, and the second lens 36 is disposed between the emission sheet 400 and the detection element 34. Thus, the sample substance in the sample cavity 52 is excited by the light of the light-emitting member 24 to emit fluorescence, the fluorescence is collected by the third lens 42, emitted to the dichroic mirror 200, emitted to the emission sheet 400 through the dichroic mirror 200, emitted to the second lens 36 after passing through the emission sheet 400, and focused on the detection element 34 through the second lens 36, so that the detection precision and sensitivity are improved.
Alternatively, the second lens 36 is fixedly mounted on the third mounting unit 32. In this way, the integration of the second lens 36 into the detection module 30 is achieved with the third mounting unit 32.
In still another embodiment, as shown in fig. 2 to 4, the optical detection apparatus further includes a first lens 26, the first lens 26 is disposed between the exciting sheet 300 and the light-emitting member 24; the optical detection device further comprises a second lens 36, the second lens 36 being arranged between the emission patch 400 and the detection element 34. Therefore, when the optical detection device is applied to in-vitro diagnosis and analysis, light rays emitted by the light-emitting component 24 are collected by the first lens 26 and then emitted to the excitation sheet 300, so that the light rays are favorably focused and emitted to the dichroic mirror 200, and are reflected by the dichroic mirror 200 and emitted to the sample cavity 52; the sample substance in the sample cavity 52 is excited by the light of the light-emitting component 24 to emit fluorescence, the fluorescence is emitted to the dichroic mirror 200, is emitted to the emission sheet 400 through the dichroic mirror 200, is emitted to the second lens 36 after passing through the emission sheet 400, and is focused on the detection element 34 through the second lens 36, so that the detection precision and sensitivity are improved.
As shown in fig. 3 to 6, on the basis of any of the above embodiments, in an embodiment, the first mounting unit 100 is provided with an optical path channel 110, the optical path channel 110 includes a first channel 112, a second channel 114 and a common channel 116, the first channel 112 and the second channel 114 are staggered and perpendicular, and one end of the first channel 112 and one end of the second channel 114 are both communicated with one end of the common channel 116 to form a common cavity 118; lens module 10 corresponds to optical path channels 110 one-to-one, dichroic mirror 200 is disposed in common cavity 118, excitation sheet 300 is disposed in first channel 112 and forms an excitation optical path with dichroic mirror 200, and emission sheet 400 is disposed in second channel 114 and forms an emission optical path with dichroic mirror 200. In this way, in use of the optical detection apparatus, the first mounting unit 100 is used to form the optical path channel 110 including the first channel 112, the second channel 114 and the common channel 116, and then the dichroic mirror 200, the excitation sheet 300 and the emission sheet 400 are integrated into the optical path channel 110, such that the excitation sheet 300 and the dichroic mirror 200 form an excitation optical path, the emission sheet 400 and the dichroic mirror 200 form an emission optical path, and the light-emitting members 24 and the detection elements 34 are arranged at intervals along the movement track of the first mounting unit 100. Therefore, the switching of the optical path 110 can be realized only by rotating or moving the first mounting unit 100 by the driver, so that the optical path 110 can be selectively butted with the illuminating member 24 and the detecting element 34 to realize the detection of different types of target objects of the target sample, and in the process, the light source module 20 and the detecting element 34 do not need to be rotated, the winding problem does not exist, and therefore, a winding avoiding space does not need to be arranged. The optical detection device adopts the lens module 10, can independently drive the lens module 10 to move, can avoid the winding problem, has more flexible design, and is favorable for the miniaturization development of an in vitro diagnosis and analysis system.
Specifically, when the optical detection device is used for in vitro diagnostic analysis in combination with the first lens 26 and the second lens 36, light emitted by the light-emitting element 24 passes through the first lens 26 and the excitation sheet 300 and then is emitted to the dichroic mirror 200, the light is reflected by the dichroic mirror 200 and then is emitted to the sample cavity 52 (in this process, the third lens 42 may be used for focusing), a sample in the sample cavity 52 is excited by light of the light-emitting element 24 to emit fluorescence, the fluorescence is collected by the third lens 42, is emitted to the dichroic mirror 200, is emitted to the emission sheet 400 by the dichroic mirror 200, passes through the emission sheet 400 and then is emitted to the second lens 36, and the fluorescence is focused on the detection element 34 by the second lens 36. After the detection of one type of object is completed, the driver drives the first mounting unit 100 to rotate, so that the optical path 110 corresponds to the other light-emitting member 24 and the other detecting element 34, and the above operation is continued to complete the detection of the other type of object.
On the basis of any of the above embodiments, as shown in fig. 5 and fig. 6, in an embodiment, the first mounting unit 100 includes a light shielding cover 120, a first plate 130 and a second plate 140, the light shielding cover 120 is provided with an optical path channel 110, at least two light shielding covers 120 are sandwiched between the first plate 130 and the second plate 140 at intervals, the first plate 130 is disposed above the light shielding cover 120, the first plate 130 is provided with a first through hole 132 communicated with the second channel 114, and the second plate 140 is provided with a second through hole 142 communicated with the common channel 116. Thus, the combination of the light shield 120 and the first and second plate bodies 130 and 140 is used to form at least two light paths 110, which facilitates the installation of the lens module 10 and the design and combination. So that the first plate 130 and the second plate 140 can move together with the lens module 10.
Further, as shown in fig. 1 to 5, in an embodiment, the number of the optical path channels 110 is at least two. In this way, the optical path channels 110 can correspond to the detection modules 30 and the light source modules 20 one to one, so that different types of target detection objects of different samples can be detected, and at least two types of target detection objects of at least two chambers can be detected at one time; meanwhile, the light path channel is arranged in the light shield, so that light pollution can be avoided, and the detection precision is influenced.
Further, as shown in fig. 5 and 6, in an embodiment, at least two light shields 120 are sandwiched between the first plate 130 and the second plate 140 at intervals along the same circumference to form a set of mounting modules, and the first mounting unit 100 includes one or more than two sets of mounting modules. Thus, the installation module can be formed, and the modular assembly is convenient.
Further, the first mounting unit 100 includes two or more sets of mounting modules arranged in a vertically stacked manner. So, can make full use of optical detection device's vertical space, can vertical stack set up more than two sets of installation module, and each other do not influence, be favorable to more light source module and the detection module of integration, be convenient for improve detection efficiency. Meanwhile, the modular assembly can be carried out, which is beneficial to reducing the manufacturing cost.
Of course, in other embodiments, other means may be utilized to form the desired structure on the first mounting unit 100.
In addition to any of the above embodiments, in one embodiment, the lens assembly 200 further includes a first light-folding member disposed in the first channel 112 and between the excitation plate 220 and the dichroic mirror 210 or between the excitation plate 220 and the first lens 26; or the lens assembly 200 further includes a second light-folding element disposed in the second channel 114 and disposed between the emission sheet 230 and the dichroic mirror 210 or between the emission sheet 230 and the second lens 36; the lens assembly 200 further includes a first light-folding member disposed in the first channel 112 and between the excitation sheet 220 and the dichroic mirror 210; the lens assembly 200 further includes a second light-folding member disposed in the second channel 114 and between the emission patch 230 and the dichroic mirror 210. Therefore, the change of the light path is realized by the first light folding part or/and the second light folding part, so that the detection unit 40 and the light emitting unit 30 are more flexibly arranged, and the internal space of the in vitro diagnosis and analysis system can be flexibly arranged.
It should be noted that the "first light-folding member" and the "second light-folding member" include, but are not limited to, any prior art implementation that can implement a light path change, such as a mirror, a prism, and an optical fiber.
In addition to any of the above embodiments, as shown in fig. 3, in an embodiment, the optical detection apparatus further includes a third lens 42, and the third lens 42 is disposed between the dichroic mirror 200 and the sample tray 50. The third lens 42 is used to better focus the light into the sample cavity 52 of the sample plate 50.
Alternatively, the third lens 42 may be directly integrated into the first mounting unit 100, moving synchronously with the first mounting unit 100.
Alternatively, as shown in fig. 1, in an embodiment, the optical detection apparatus further includes an integration 40, the third lens 42 is fixed in the integration 40, and the integration 40 is fixed between the first mounting unit 100 and the sample tray 50. In this way, the third lens 42 can be modularly assembled using the integration 40, facilitating the modular assembly.
Of course, the integrated member 40 may be further integrated to the first mounting unit 100 and may move synchronously with the first mounting unit 100.
Of course, in other embodiments, the third lens 42 may be integrated into the sample tray 50; or other mounting structure.
The "first mounting unit 100" may be any mounting structure capable of mounting the above-described components, such as a mounting bracket, a mounting seat, a mounting frame, and a mounting case.
The "second mounting unit 22" may be any mounting structure capable of mounting the above-described components, such as a mounting bracket, a mounting seat, and a mounting case.
The "second mounting unit 22" may be any mounting structure capable of mounting the above-described components, such as a mounting bracket, a mounting seat, a mounting plate, and a mounting case.
As shown in fig. 2 and 3, in one embodiment, the light emitting members 24 are spaced apart from each other along the same circumference, the detecting elements 34 are spaced apart from each other along another circumference, and the driver is used for driving the first mounting unit 100 to rotate along the center of the same circumference. Therefore, detection of different target detection objects can be realized only by rotation, and the detection device is simple to control and easy to realize; in the process, the light source module 20 and the detection element 34 do not need to rotate, and the winding problem does not exist, so that a winding avoiding space does not need to be arranged, and the volume of the optical detection device can be smaller. Meanwhile, the at least two light shields 120 are clamped between the first plate 130 and the second plate 140 at intervals along the same circumference to form a set of mounting modules, and the first mounting unit 100 includes at least two sets of mounting modules vertically stacked. Thus, more elements can be integrated with rotation, enabling simultaneous detection of more sample chambers 52.
Of course, in another embodiment, the light emitting members 24 are spaced apart from each other along a straight line, the detecting members 34 are spaced apart from each other along another straight line, and the driver is used to drive the first mounting unit 100 to reciprocate along the straight line. Therefore, detection of different target detection objects can be realized only by reciprocating along the linear direction; in the process, the light source module 20 and the detection module 30 do not need to rotate, and the winding problem does not exist, so that a winding avoiding space does not need to be arranged, and the size of the optical detection device can be smaller.
In another aspect, the present application further provides an in vitro diagnostic and analytical system, comprising the optical detection device in any of the above embodiments.
The in vitro diagnosis and analysis system applies the optical detection device, can independently drive the lens module 10, the light source module 20 or the detection module 30 to move, and is more flexible in design and easier to control.
Further, the in-vitro diagnostic and analytical system further comprises a sample tray 50, the sample tray 50 is provided with detection cavities corresponding to the free ends of the common channels 116 one by one, and a third lens 42 is arranged between the detection cavities and the common channels 116. Thus, light emitted by the light emitting element 24 passes through the first lens 26 and the excitation sheet 300 and then is emitted to the dichroic mirror 200, reflected by the dichroic mirror 200 and then is collected by the third lens 42 and then emitted to the sample cavity 52, a sample in the sample cavity 52 is excited by light of the light emitting element 24 to emit fluorescence, the fluorescence is emitted to the dichroic mirror 200, emitted to the emission sheet 400 through the dichroic mirror 200, emitted to the second lens 36 through the emission sheet 400, and then focused on the detection element 34 through the second lens 36. After the detection of one type of object is completed, the driver drives the first mounting unit 100 to rotate, so that the optical path 110 corresponds to another light emitting element and another detecting element, and the above operation is continued to complete the detection of another type of object.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (13)
1. An optical inspection apparatus, comprising:
the lens module comprises a first mounting unit, a dichroic mirror, an excitation sheet and an emission sheet, wherein the dichroic mirror and the excitation sheet are arranged in the first mounting unit at intervals and matched with the excitation sheet to form an excitation light path;
the light source module comprises a second mounting unit and at least two light-emitting pieces, the light-emitting pieces are arranged on the second mounting unit at intervals, and the light-emitting pieces and the excitation sheet are arranged at intervals;
the detection module comprises a third installation unit and at least two detection elements, the detection elements are arranged on the third installation unit at intervals, and the detection elements and the emission sheet are arranged at intervals; and
the driver is used for driving the first mounting unit, driving the first mounting unit and the second mounting unit or driving at least one of the first mounting unit and the third mounting unit to move.
2. The optical inspection device of claim 1, wherein the light emitting members are spaced apart from each other along a same circumference, the inspection elements are spaced apart from each other along another circumference, and the driver is configured to drive the first mounting unit to rotate along a center of the same circumference.
3. The optical inspection device of claim 1, wherein the light emitting elements are spaced apart from each other along a straight line, the inspection elements are spaced apart from each other along another straight line, and the actuator is configured to drive the first mounting unit to reciprocate along the straight line.
4. The optical inspection device of claim 1, further comprising a first lens disposed between the excitation sheet and the light emitter; and/or the optical detection device further comprises a second lens, and the second lens is arranged between the emission sheet and the detection element.
5. The optical detection device according to any one of claims 1 to 4, wherein the first mounting unit is provided with an optical path channel, the optical path channel comprises a first channel, a second channel and a common channel, the first channel and the second channel are staggered, and one end of the first channel and one end of the second channel are both communicated with one end of the common channel and form a common cavity; the lens modules correspond to the light path channels one to one, the dichroic mirror is arranged in the shared cavity, the excitation sheet is arranged in the first channel and forms an excitation light path with the dichroic mirror, and the emission sheet is arranged in the second channel and forms an emission light path with the dichroic mirror.
6. The optical detection device according to claim 5, wherein the first mounting unit includes a light shield, a first plate and a second plate, the light shield has one of the light path channels, the light shield is interposed between the first plate and the second plate at an interval, the first plate is disposed above the light shield, the first plate has a first through hole communicating with the second channel, and the second plate has a second through hole communicating with the common channel.
7. The optical inspection device of claim 6, wherein the number of the optical paths is at least two, and the light shielding covers correspond to the optical paths one by one.
8. The optical inspection device of claim 7, wherein at least two light shields are sandwiched between the first board and the second board at intervals along the same circumference to form a set of mounting modules, and the first mounting unit includes one or more sets of the mounting modules.
9. The optical inspection device of claim 8, wherein the first mounting unit includes more than two sets of the mounting modules arranged in a vertical stack.
10. The turntable module of claim 5, wherein the lens assembly further comprises a first light-folding member disposed within the first channel and disposed between the excitation sheet and the dichroic mirror or between the excitation sheet and the first lens; and/or the lens assembly further comprises a second light folding part, wherein the second light folding part is arranged in the second channel and is arranged between the emission sheet and the dichroic mirror or between the emission sheet and the second lens.
11. The optical detection device of claim 5, further comprising a third lens disposed between the dichroic mirror and the sample tray.
12. The optical detection device of claim 10, further comprising an integrated piece, wherein the third lens is fixedly arranged in the integrated piece, and the integrated piece is fixedly arranged between the first mounting unit and the sample tray.
13. An in vitro diagnostic assay system comprising an optical detection device according to any one of claims 1 to 12.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010951053.7A CN112161927A (en) | 2020-09-11 | 2020-09-11 | In-vitro diagnosis and analysis system and optical detection device |
| PCT/CN2020/138007 WO2022052363A1 (en) | 2020-09-11 | 2020-12-21 | In vitro diagnostic analysis system, and optical detection apparatus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010951053.7A CN112161927A (en) | 2020-09-11 | 2020-09-11 | In-vitro diagnosis and analysis system and optical detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022052362A1 (en) * | 2020-09-11 | 2022-03-17 | 广州万孚生物技术股份有限公司 | In vitro diagnostic analysis system, optical detection apparatus, and motion disc module |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US7295316B2 (en) * | 2004-01-14 | 2007-11-13 | Applera Corporation | Fluorescent detector with automatic changing filters |
| EP2406614A1 (en) * | 2009-03-10 | 2012-01-18 | Agency For Science, Technology And Research | Apparatus for processing a biological and/or chemical sample |
| JP6351765B2 (en) * | 2015-01-27 | 2018-07-04 | 株式会社日立ハイテクノロジーズ | Multicolor fluorescence analyzer |
| CN107478629A (en) * | 2017-09-04 | 2017-12-15 | 中国科学院苏州生物医学工程技术研究所 | A kind of large area digital pcr droplet fluorescence high pass amount detecting device and method |
| KR102133633B1 (en) * | 2018-04-17 | 2020-07-13 | (주)로고스바이오시스템스 | A device for real-time detecting nucleic acids amplification products |
| CN110161003B (en) * | 2019-05-17 | 2022-07-22 | 深圳市刚竹医疗科技有限公司 | Optical detection device and real-time fluorescence quantitative nucleic acid amplification detection system |
| US10520437B1 (en) * | 2019-05-24 | 2019-12-31 | Raytheon Company | High sensitivity sensor utilizing ultra-fast laser pulse excitation and time delayed detector |
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| WO2022052362A1 (en) * | 2020-09-11 | 2022-03-17 | 广州万孚生物技术股份有限公司 | In vitro diagnostic analysis system, optical detection apparatus, and motion disc module |
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