CN211227169U - Multichannel fluorescence quantitative PCR detection system - Google Patents
Multichannel fluorescence quantitative PCR detection system Download PDFInfo
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- CN211227169U CN211227169U CN201922149134.0U CN201922149134U CN211227169U CN 211227169 U CN211227169 U CN 211227169U CN 201922149134 U CN201922149134 U CN 201922149134U CN 211227169 U CN211227169 U CN 211227169U
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Abstract
The utility model relates to a multichannel fluorescence quantitative PCR detection system, which comprises a PCR mechanism and a fluorescence detection mechanism, wherein the PCR mechanism is provided with a temperature-changing module, a sample hole for installing a tube to be tested is arranged on the temperature-changing module, the bottom of the temperature-changing module is provided with a first light transmission hole communicated with the sample hole, and a side surface of the temperature-changing module is provided with a second light transmission hole communicated with the sample hole; the fluorescence detection mechanism includes: the excitation assembly emits excitation light into the first light transmission hole, and the detection assembly detects a fluorescence signal in the second light transmission hole. The first light hole of the temperature changing module of the PCR mechanism receives exciting light from the exciting assembly, and fluorescence is emitted from the second light hole to be detected by the detecting assembly. The test tube top and the ambient light of bottom all can't get into the test section, have reduced the light source background in the signal promptly, promote the accuracy that detects, and every first light trap has the same excitation efficiency and detection sensitivity, has eliminated the influence of photoelectric device performance drift.
Description
Technical Field
The utility model relates to a molecular biology experiment, gene amplification technology, fluorescence quantitative determination and diagnosis technical field especially relate to a multichannel fluorescence quantitative PCR detecting system.
Background
With the development of molecular biology research and application, the PCR (Polymerase Chain Reaction) technology has gained wide acceptance due to its accuracy and reliability, and the DNA and RNA detection technology developed on this basis is a fundamental stone of molecular biology experiments, and also a tool for human to know the microscopic world and understand itself.
The optical system working modes of the current mainstream fluorescent quantitative PCR detection system are as follows:
1. top detection mode. The excitation light source and the optical detection device are positioned above the temperature changing module, and the semiconductor refrigerator is arranged at the bottom of the temperature changing module. The disadvantages are that: water vapor, dirt or marks on the top of the plastic test tube can affect the detection of the fluorescent signal; multiple independent excitation and detection devices, optical distortions are present.
2. Bottom detection mode. The bottom of the temperature changing module is provided with a semiconductor refrigerator, excitation light and fluorescence signals are conducted through an optical fiber light guide beam led out from the bottom of the module hole, and excitation and fluorescence are realized through a dichroic mirror. The advantages are that: moisture, dirt or marks on the top of the plastic test tube have no influence on the detection result; the uniform excitation and detection device ensures that the LED light performance of each hole is completely consistent, and the detectors realize the identity, so that the relative drift of the fluorescence of each hole in the temperature change module is minimized. The disadvantages are as follows: the semiconductor refrigerator needs to be punched, so that the temperature rising and falling speed of the module is influenced, and the failure rate is high due to complex process; the dichroic mirror is used, so that the background of an excitation light source cannot be avoided to be mixed in the fluorescent signal, and the signal quality and the purity are influenced; the optical system has the risk of dust accumulation and higher maintenance requirement.
3. Side detection mode. Fluorescence signal was detected from the side, with the following advantages: the bottom of the temperature changing module is closed, so that the design of the semiconductor refrigerator is not influenced; the detection optical path is short, and the fluorescence signal is strong; the test tube is suitable for various types. The disadvantages are as follows: there are requirements for the number and arrangement of the module holes.
As can be seen from the above, PCR detection from various positions has advantages and disadvantages, and thus some improvements are required.
SUMMERY OF THE UTILITY MODEL
In view of the above, it is necessary to provide a multi-channel fluorescence quantitative PCR detection system. The influence of the performance drift of the photoelectric device can be eliminated without periodic calibration, and the ambient light background in the fluorescent signal is greatly eliminated.
A multi-channel fluorescence quantitative PCR detection system comprises a PCR mechanism and a fluorescence detection mechanism, and comprises:
the PCR mechanism is provided with a temperature changing module, a sample hole for installing a tube to be tested is formed in the temperature changing module, a first light transmission hole communicated with the sample hole is formed in the bottom of the temperature changing module, and a second light transmission hole communicated with the sample hole is formed in one side face of the temperature changing module;
the fluorescence detection mechanism includes: the excitation assembly is used for emitting exciting light into the first light transmission hole, and the detection assembly is used for detecting a fluorescence signal in the second light transmission hole.
According to the multichannel fluorescent quantitative PCR detection system, the temperature-changing module of the PCR mechanism receives exciting light from the exciting assembly through the first light hole at the bottom, and emits fluorescent light from the second light hole on the side surface to be detected by the detection assembly. Exciting light gets into the test tube that the sample hole shines and is equipped with the reagent from first light trap, need not to use the dichroic mirror, and the ambient light of test tube top and bottom all can't get into the test section through refraction, reflection, has reduced the light source background in the signal promptly, promotes the accuracy that detects, and when scanning first light trap, every first light trap has the same excitation efficiency and detectivity, detects the influence of having eliminated photoelectric device performance drift relatively. The side detection of the fluorescence signal has the characteristics of optical path end detection and strong fluorescence signal.
In one embodiment, the PCR mechanism further comprises: the temperature control module and the second light holes are respectively located on two opposite side surfaces of the temperature changing module, and the temperature control module is used for adjusting the temperature of the temperature changing module.
In one embodiment, the temperature control module comprises: the refrigerator and the second light transmission hole are respectively positioned on two opposite side surfaces of the temperature changing module, and the refrigerator is positioned between the radiator and the temperature changing module.
In one embodiment, the fluorescence detection mechanism further comprises: the two excitation assemblies are formed and are arranged on the bottom plate, and the light paths of the two excitation assemblies are parallel; the detection assemblies are also formed in two numbers, and respectively correspond to the two excitation assemblies.
In one embodiment, the optical paths of the detection components and the corresponding excitation components are located on the same plane. The excitation component and the light path middle shaft of the detection component are coplanar to form a fluorescence detection channel.
In one embodiment, the optical path of the detection assembly is at an angle of 90 ° with respect to the optical path of the corresponding excitation assembly. The included angle between the light path of the detection component and the light path of the excitation component is 90 degrees, and the included angle between the axis of the first light hole and the axis of the second light hole is 90 degrees. So that the excitation light and the environment light passing through the top of the test tube can not enter the detection part through refraction and reflection, namely, the light source background in the signal is reduced. Thereby improving the accuracy of fluorescence detection.
In one embodiment, the sample holes are provided with a plurality of sample holes which are arranged on the temperature change module, the number of the first light transmission holes and the number of the second light transmission holes are the same as the number of the sample holes, and any one sample hole is respectively communicated with one first light transmission hole and one second light transmission hole;
the fluorescence detection mechanism further includes:
the connecting frame is connected between the excitation assembly and the detection assembly;
and the sliding assembly is used for driving the excitation assembly and the detection assembly to slide along the arrangement direction of the sample holes.
In one embodiment, each of the sample wells is arranged in a straight line, or in an arc, or in a spiral.
In one embodiment, a gap is formed between the detection assembly and the temperature change module, and a gap is formed between the excitation assembly and the temperature change module.
In one embodiment, the distance between the optical paths of two of the excitation assemblies is equal to 1.5 times the distance between adjacent light-transmissive holes.
Drawings
FIG. 1 is a top view of the PCR mechanism being tested by the PCR fluorescence testing mechanism;
FIG. 2 is a side sectional view of the PCR mechanism being tested by the PCR fluorescence testing mechanism;
FIG. 3 is an exploded view of a PCR fluorescence detection mechanism;
FIG. 4 is a cross-sectional view of the PCR mechanism in a plan view when the PCR mechanism is detected by the PCR fluorescence detecting mechanism.
Wherein: 1. a temperature changing module; 11. a sample well; 101. a refrigerator; 102. a heat sink; 12. a first light-transmitting hole; 13. a second light-transmitting hole; 2. an excitation assembly; 21. an excitation light source; 22. exciting an optical prism set; 23. An excitation light filter; 3. a detection component; 31. detecting an optical prism group; 32. a fluorescent filter; 33. a photosensor; 34. a large area photosensor; 4. a base; 61. a motor; 62. a drive shaft; 63. a fixed shaft; 64. a first belt; 65. a second belt; 7. a connecting frame; 8. a first fluorescence detection channel; 9. a second fluorescence detection channel.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail with reference to the accompanying drawings and the following detailed description. It should be understood that the detailed description and specific examples, while indicating the scope of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The utility model discloses a first embodiment discloses a multichannel fluorescence quantitative PCR detecting system for carry out PCR's fluorescence detection.
The multichannel fluorescence quantitative PCR detection system comprises: the device comprises a PCR mechanism and a fluorescence detection mechanism, wherein the PCR mechanism is used for fixing a test tube filled with a PCR reagent, and the fluorescence detection mechanism is used for carrying out fluorescence detection on the test tube on the PCR mechanism.
As shown in fig. 1, the PCR mechanism includes: the test tube temperature control device comprises a temperature changing module 1 and a temperature control module arranged on the temperature changing module 1, wherein the test tube is arranged on the temperature changing module, and the temperature control module can adjust the temperature of the temperature changing module 1 so as to complete the detection of the test tube at a proper temperature. Referring to fig. 2, a plurality of test tube sample holes 11 are formed in the temperature changing module 1 at equal intervals along the horizontal direction, each PCR reaction test tube is inserted into each sample hole 11, and two light holes, namely a first light hole 12 and a second light hole 13, communicated with the sample holes 11 are formed in the temperature changing module 1. The first light hole 12 is formed in the bottom surface of the temperature change module 1, the second light hole 13 is formed in the side surface of the temperature change module 1, and the first light hole 12 and the second light hole 13 are used for the PCR fluorescence detection mechanism to detect.
Temperature control module and second light trap 13 are located the relative two sides of alternating temperature module 1 respectively, and the dorsal part at alternating temperature module 1 is established to the temperature control module, does not influence fluorescence detection mechanism 3's scanning, and wherein the temperature control module includes: a heat sink 102, a refrigerator 101 fixed to the heat sink 102, the refrigerator 101 abutting against the back side of the temperature change module 1. Because the first light hole 12 and the second light hole 13 are respectively arranged at the bottom and the side of the temperature changing module 1, the temperature control module arranged at the back side of the temperature changing module 1 does not need to be provided with holes for light to pass through, so that the process difficulty is reduced, the temperature control module can keep a good temperature regulation rate, and the efficiency of the whole experiment is improved. In this embodiment, the refrigerator 101 is a semiconductor refrigerator 101, and when the semiconductor refrigerator 101 is powered on, the heat radiator 102 can increase the heat dissipation speed of the temperature change module 1, accelerate the lowering of the temperature change module 1, and quickly adjust the temperature of the temperature change module 1 to a temperature suitable for testing.
The fluorescence detection mechanism 3 includes: excite subassembly 2 and determine module 3, excite subassembly 2 and determine module 3 and be used for respectively corresponding with first light trap 12 and second light trap 13 on the alternating temperature module 1, excite subassembly 2 and send the exciting light, shine the test tube through first light trap 12, the reagent in the test tube is received after being shone by the exciting light and is aroused, send the fluorescence of specific wavelength, determine module 3 detects the fluorescence that the reagent in the test tube sent through second light trap 13.
As shown in fig. 3, the fluorescence detection mechanism 3 further includes: the base 4, the excitation subassembly 2 is established on the base 4, in this embodiment, be equipped with two excitation subassemblies 2 on the base 4, detection component 3 also is equipped with two, with excite subassembly 2 one-to-one, wherein the light path axis coplane of corresponding excitation subassembly 2 and detection component 3 forms a fluorescence detection passageway, two fluorescence detection passageways are formed altogether in this embodiment, certainly, the quantity of exciting subassembly 2 also can be more than two passageways, only use 2 as the example in this embodiment, do not specifically limit. The two detection units 3 may share one large-area photosensor 34, or may use separate photosensors 33. The excitation assembly 2 emits excitation light to irradiate the test tube from the first light hole 12 at the bottom of the temperature changing module 1, the test tube is excited by the excitation light to emit fluorescence, and the fluorescence enters the detection assembly through the second light hole 13 on the side surface of the temperature changing module 1 to realize the detection of one PCR test tube. Excitation light and fluorescence pass through first light trap 12 and second light trap 13 respectively, need not to use dichroic mirror, and the test tube top and the ambient light of bottom all can't get into the detection part through refraction, reflection, have reduced the light source background in the signal promptly, promote the accuracy that detects. The fluorescence received from the second light-transmitting hole 13 on the side surface has the advantages of short optical path, strong fluorescence signal and high detection sensitivity. And the side detection greatly reduces the ambient light factor background in the signal.
The excitation assembly 2 comprises: excitation light source 21, exciting optical prism group 22 and exciting light filter 23 constitute, and detection component 3 includes: the detection optical prism group 31, the fluorescence filter 32 and the photoelectric sensor 33, wherein the excitation light filter 23 and the fluorescence filter 32 have corresponding excitation light wavelength and fluorescence wavelength for a specific reagent luminescent dye or probe, and the filter cross cut depth reaches above OD6, thereby obtaining a high-purity fluorescence signal. The excitation optical prism group 22 and the detection optical prism group 31 are used for transmitting excitation light or fluorescence on an optical path, and the photoelectric sensor 33 is used for detecting fluorescence. The light spot sensor 33 employs: the photomultiplier has extremely high sensitivity and ultra-fast response, can measure weak optical signals, and improves the detection accuracy.
It should be noted that, in this embodiment, an included angle between the light path of the detecting component 3 and the light path of the exciting component 2 is 90 °, and an included angle between the axis of the first light-transmitting hole 12 and the axis of the second light-transmitting hole 13 is also 90 °, so that both the exciting light and the ambient light passing through the top of the test tube cannot enter the detecting portion through refraction and reflection, that is, the light source background in the signal is reduced, thereby improving the accuracy of fluorescence detection.
As shown in fig. 1 and 2, in the temperature change module 1 of the PCR mechanism, each sample hole 11 is arranged in the horizontal direction, each first light-transmitting hole 12 is arranged in the horizontal direction, each second light-transmitting hole 13 is arranged in the horizontal direction, each sample hole 1 is connected with one first light-transmitting hole 12 and one second light-transmitting hole 13, and the connecting line of each sample hole 11, the connecting line of each first light-transmitting hole 12 and the connecting line of each second light-transmitting hole 13 are parallel. The fluorescence detection mechanism 3 further includes: link 7 and the subassembly that slides, link 7 are connected and are aroused between subassembly 2 and the determine module 3, will arouse each subassembly 2 and be connected with each determine module 3, make to arouse subassembly 2 and determine module 3 integrative, remain relative position and relative contained angle throughout. The excitation assembly 2 and the detection assembly 3 can be fixed on the connecting frame 7 at the same time, and can also be integrated on the connecting frame 7 in a solid state.
The sliding component is connected with the connecting frame 7, and can drive the connecting frame 7, the excitation component 2 and the detection component 3 to move together along the horizontal direction, so that the reagents in the test tubes on the PCR mechanism are subjected to fluorescence detection. Wherein arouse each first light trap 12 of subassembly 2 scanning in proper order, detect component 3 scans each second light trap 13 in proper order, realizes the detection of every test tube. It should be noted that the respective mounting members 11, the first light-transmitting holes 12 and the second light-transmitting holes 13 may be arranged along other paths, such as along an arc or a spiral, and the scanning path of the fluorescence detection device changes when the shape of the arrangement path of the sample holes 11 changes. When the excitation component 2 scans each first light hole 12, a certain gap is always formed between the excitation component and the first light hole 12, so that the temperature change module 1 is prevented from being touched, and when the detection component 3 scans each second light hole 13, a certain gap is also always formed between the detection component and the second light hole 13. All the first light holes 12 have the same excitation efficiency and detection sensitivity, the influence of photoelectric device performance drift is eliminated in relative detection, the service life is prolonged, frequent periodic calibration is not needed, data have reliability and reproducibility, and the effectiveness of comparison between a sample and a reference product in fluorescent quantitative PCR detection is guaranteed.
The sliding component may be any mechanism that drives the connecting frame 7 to move, such as a rack and pinion driving mechanism, a worm and gear driving mechanism, and the like, and the sliding component in this embodiment is a belt conveying mechanism, as shown in fig. 4, the sliding component includes: the motor 61, a transmission shaft 62 parallel to the output shaft of the motor 61, and a fixed shaft 63 opposite to the transmission shaft 62, wherein the output shaft of the motor 61 and the transmission shaft 62 are connected by a first belt 64, so that the transmission shaft 62 and the output shaft can rotate synchronously. Meanwhile, the transmission shaft 62 is connected with the fixed shaft 63 through a second belt 65, and the second belt 65 is arranged along the horizontal direction. The motor 61 during operation can drive the second belt 65 motion between transmission shaft 62 and the fixed axle 63, and the link 7 is connected with the second belt 65, drives detection component 3 and arouses the motion of subassembly 2 under the drive of second belt 65, loops through the test tube in each sample hole 11, tests the reagent in each test tube in proper order. Compared with the prior art in which the fluorescence detection channel is switched by the motor 61 and the filter wheel, the detection assembly 3 and the excitation assembly 2 in the embodiment respectively correspond to the second light hole 13 and the first light hole 12, and the fluorescence filter 32 has a corresponding fluorescence wavelength for a specific reagent luminescent dye or probe, so that the switching difficulty of the fluorescence detection channel is reduced, the fluorescence detection channel is more convenient to replace a detection object, and the experimental efficiency is improved.
As shown in fig. 3, the two detection assemblies 3 in the fluorescence detection device and the respective corresponding excitation assemblies 2 form a first fluorescence detection channel 8 and a second fluorescence detection channel 9, so as to perform two detections on the reagents in each test tube, thereby improving the accuracy of the experimental results. The fluorescence detection device can simultaneously open two fluorescence detection channels to detect the reagents in each test tube, so that the detection efficiency is improved; or the detection can be performed twice, and one fluorescence detection channel is opened for the reagent in each test tube. The PCR detection assembly further comprises: and a main control module, which is electrically connected with the excitation light source 21, the photoelectric sensor 33 and the motor 61 and controls the opening and closing of the excitation assembly 2, the detection of the detection assembly 3 and the movement of the sliding assembly, in combination with fig. 1.
As shown in fig. 2, each time a fluorescence detection channel is opened, the fluorescence detection apparatus turns on one excitation assembly 2 and turns off the other excitation assembly 2 during the first round of test, and at this time, the signal obtained by the light spot sensor of the detection assembly 3 corresponding to the opened excitation assembly 2 is the fluorescence signal of the fluorescence detection channel; and after all the reagents in the test tubes are detected, performing a second round of test, and sequentially detecting the reagents in the test tubes by using the excitation assembly 2 which is closed in the first round alone, wherein the signals obtained by the light spot sensors of the detection assemblies 3 corresponding to the excitation assembly 2 are fluorescence signals of the fluorescence detection channels. And completing the detection of the reagents in the test tubes by the two detection channels respectively.
When the fluorescence device simultaneously opens two fluorescence detection channels for detection, two excitation assemblies 2 are simultaneously opened, as shown in fig. 4, the distance between the light paths of the two excitation assemblies 2 is equal to 1.5 times the distance between the adjacent light holes. When the connecting frame 7 moves along the horizontal direction, only one excitation assembly 2 is always aligned with one first light-transmitting hole 12 of the PCR mechanism, and the other excitation assembly 2 is staggered with the first light-transmitting hole 12. The two fluorescence detection channels are used for sequentially detecting each test tube, the two fluorescence channels alternately generate fluorescence signals, signal sequences of the channels can be obtained through time-sharing processing, detection is completed, and low background fluorescence and low inter-channel crosstalk can be obtained during detection.
In the fluorescence detection system in this embodiment, the excitation component 2 and the detection component 3 respectively scan at the bottom and the side of the temperature changing module 1, so that the fluorescence detection system has the advantages of short optical path and rapidness, reduces the interference of background light, and improves the detection accuracy. All the first light holes 12 have the same excitation efficiency and detection sensitivity, the influence of photoelectric device performance drift is eliminated in relative detection, the service life is prolonged, frequent periodic calibration is not needed, data have reliability and reproducibility, and the effectiveness of comparison between a sample and a reference product in fluorescent quantitative PCR detection is guaranteed.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (10)
1. The utility model provides a multichannel fluorescence quantitative PCR detecting system, includes PCR mechanism and fluorescence detection mechanism, its characterized in that includes:
the PCR mechanism is provided with a temperature changing module, a sample hole for installing a tube to be tested is formed in the temperature changing module, a first light transmission hole communicated with the sample hole is formed in the bottom of the temperature changing module, and a second light transmission hole communicated with the sample hole is formed in one side face of the temperature changing module;
the fluorescence detection mechanism includes: the excitation assembly is used for emitting exciting light into the first light transmission hole, and the detection assembly is used for detecting a fluorescence signal in the second light transmission hole.
2. The multi-channel fluorescent quantitative PCR detection system of claim 1, wherein the PCR mechanism further comprises: the temperature control module and the second light holes are respectively located on two opposite side surfaces of the temperature changing module, and the temperature control module is used for adjusting the temperature of the temperature changing module.
3. The multi-channel fluorescence quantitative PCR detection system of claim 2, wherein the temperature control module comprises: the refrigerator and the second light transmission hole are respectively positioned on two opposite side surfaces of the temperature changing module, and the refrigerator is positioned between the radiator and the temperature changing module.
4. The multi-channel fluorescence quantitative PCR detection system of claim 2, wherein the fluorescence detection mechanism further comprises: the two excitation assemblies are formed and are arranged on the bottom plate, and the light paths of the two excitation assemblies are parallel; the detection assemblies are also formed in two numbers, and respectively correspond to the two excitation assemblies.
5. The multi-channel fluorescence quantitative PCR detection system of claim 3, wherein the optical paths of the detection components and the corresponding excitation components are located on the same plane.
6. The multi-channel fluorescence quantitative PCR detection system of claim 5, wherein the included angle between the optical path of the detection component and the optical path of the corresponding excitation component is 90 °, and the included angle between the axis of the first light hole and the axis of the second light hole is 90 °.
7. The multi-channel fluorescent quantitative PCR detection system of claim 6, wherein the sample holes are provided in a plurality and arranged on the temperature varying module, and the number of the first light-transmitting holes and the number of the second light-transmitting holes are the same as the number of the sample holes, and any one sample hole is respectively communicated with one first light-transmitting hole and one second light-transmitting hole;
the fluorescence detection mechanism further includes:
the connecting frame is connected between the excitation assembly and the detection assembly;
and the sliding assembly is used for driving the excitation assembly and the detection assembly to slide along the arrangement direction of the sample holes.
8. The multi-channel quantitative fluorescence PCR detection system of claim 7, wherein each of the sample wells is arranged along a straight line, or along an arc, or along a spiral.
9. The multi-channel quantitative fluorescence PCR detection system of claim 4, wherein the distance between the optical paths of two excitation assemblies is equal to 1.5 times the distance between adjacent light-transmitting holes.
10. The multi-channel fluorescence quantitative PCR detection system of claim 4, wherein a gap is formed between the detection assembly and the temperature-changing module, and a gap is formed between the excitation assembly and the temperature-changing module.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300926A (en) * | 2020-11-20 | 2021-02-02 | 湖南开启时代生物科技有限责任公司 | (optical) detection device with temperature regulation function |
| CN112779157A (en) * | 2021-01-08 | 2021-05-11 | 鲲鹏基因（北京）科技有限责任公司 | PCR reaction device with sample preparation function |
| CN112858246A (en) * | 2021-03-14 | 2021-05-28 | 新羿制造科技（北京）有限公司 | Micro-droplet chip analyzer containing multi-light-path assembly |
| CN117286017A (en) * | 2023-11-21 | 2023-12-26 | 瓜藤生物科技(深圳)有限公司 | Multichannel nucleic acid amplification analysis equipment |
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2019
- 2019-12-04 CN CN201922149134.0U patent/CN211227169U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300926A (en) * | 2020-11-20 | 2021-02-02 | 湖南开启时代生物科技有限责任公司 | (optical) detection device with temperature regulation function |
| CN112300926B (en) * | 2020-11-20 | 2024-02-02 | 湖南开启时代生物科技有限责任公司 | Detection device with temperature regulation function |
| CN112779157A (en) * | 2021-01-08 | 2021-05-11 | 鲲鹏基因（北京）科技有限责任公司 | PCR reaction device with sample preparation function |
| CN112858246A (en) * | 2021-03-14 | 2021-05-28 | 新羿制造科技（北京）有限公司 | Micro-droplet chip analyzer containing multi-light-path assembly |
| CN117286017A (en) * | 2023-11-21 | 2023-12-26 | 瓜藤生物科技(深圳)有限公司 | Multichannel nucleic acid amplification analysis equipment |
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