CN101236153A - Absorbed light and fluorescence spectrum composite detector - Google Patents
Absorbed light and fluorescence spectrum composite detector Download PDFInfo
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- CN101236153A CN101236153A CNA2008100430381A CN200810043038A CN101236153A CN 101236153 A CN101236153 A CN 101236153A CN A2008100430381 A CNA2008100430381 A CN A2008100430381A CN 200810043038 A CN200810043038 A CN 200810043038A CN 101236153 A CN101236153 A CN 101236153A
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- 238000002189 fluorescence spectrum Methods 0.000 title claims abstract description 33
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Abstract
The invention relates to an absorbed light and fluorescence spectrum composite detector, which comprises a radiation light source, a color matching dish, a microprocessor, and an absorbed light sensor module and a fluorescence sensor module which are mutually 90 DEG to each other and are connected with the microprocessor. The microprocessor is connected with the radiation light source through a pulse drive circuit, and condensing lenses are respectively arranged between the color matching dish and the radiation light source, and between the absorbed light sensor module and the fluorescence sensor module. The radiation light source is capable of selectively giving off pulsed light with two or above two wave lengths. The light source with each selected wave length is corresponding to the absorbed light spectrum and the fluorescence spectrum of each measured object. The absorbed light and fluorescence signals of the measured objects are inputted into a personal computer with time and place selected through optoelectronic conversion, thereby solving the problem of being incapable of carrying out simultaneous and synchronous composite detection of the absorbed light and fluorescence of various objects in a sample.
Description
Technical field
The present invention relates to a kind of absorbing light and fluorescence spectrum composite detector.
Background technology
Absorption spectrum detection principle is that the light transmission of selected wavelength contains the sample of measured object, measures transmitted intensity and changes, and determine the concentration of analyte in sample according to Beer-Lambert Law.In the numerous analytical approach and instrument, typical example can be consulted PCT patented claim document WO 2004025233.
Similarly, the detection principle of fluorescence spectrum be in the sample fluorescent dye under the radiant light effect, inspire fluorescence and according to fluorescence intensity and wavelength migration and measured object corresponding fluorescence spectrum determine the concentration of measured object in sample.The method of setting up with this principle and the instrument of manufacturing, visible american documentation literature US4877965 of typical case and US5196709.
The detection of absorption spectrum is to be widely adopted one of conventional analysis means in chemistry, biochemistry, medical science and other commercial Application already.Fluorescence spectrum detects the typical case and uses then aspect the biological sample test.The amount that common biological sample uses in fluorescence analysis is fewer, for example nucleic acid and protein test.And the characteristics that fluorescence spectrum detects are to reduce to minimum to background interference.For example american documentation literature US4006360, US4341957 have relevant description with US4791310.
Summary of the invention
Technical matters to be solved by this invention is: at the problems referred to above, propose a kind ofly can realize synchronously and carry out absorbing light and fluoroscopic examination and the absorbing light and the fluorescence spectrum composite detector that can carry out correlation analysis to the result of analyte simultaneously.
Technical scheme of the present invention:
Absorbing light and fluorescence spectrum composite detector, comprise radiating light source, cuvette and microprocessor, the position is 90 absorbing light sensor assembly and the fluorescent optical sensor modules of spending and linking to each other with described microprocessor each other, described microprocessor links to each other with described radiating light source by pulse driving circuit, is respectively equipped with focus lamp between described cuvette and described radiating light source, absorbing light sensor assembly and the fluorescent optical sensor module;
Described microprocessor is used to set and export the pulse interval that produces absorbing light, fluorescence respectively and the steering order of pulse strength;
Described pulse driving circuit is used for the steering order according to described microprocessor, makes described radiating light source select different optical wavelength and light intensity output in the different burst lengths;
Described absorbing light sensor assembly comprises the amplifying circuit that links to each other with the absorbing light sensor, is used for the steering order according to described microprocessor, becomes electric signal to output to described microprocessor the absorbing light conversion of signals in the light absorbing burst length;
Described fluorescent optical sensor module comprises the amplifying circuit that links to each other with fluorescent optical sensor, is used for the steering order according to described microprocessor, converts fluorescence signal to electric signal and output to described microprocessor in the burst length of fluorescence.
Described pulse driving circuit comprises ON-OFF control circuit and current control circuit; The input end of field effect transistor links to each other with described microprocessor in the described ON-OFF control circuit, and output terminal links to each other with described radiating light source; Described current control circuit is made of the field effect transistor and the peripheral circuit thereof that link to each other with described microprocessor.
Between described focus lamp and described absorbing light sensor, can be provided with light separator.
Between described focus lamp and described fluorescent optical sensor, can be provided with light separator.
Described absorbing light sensor is PiN photodiode, photoelectricity coupling array sensor, photodiode arrangement or photoelectricity vacuum tube.
Described fluorescent optical sensor is PiN photodiode, photoelectricity coupling array sensor, photodiode arrangement or photoelectricity vacuum tube.
Described light separator is wave filter, grating or monochromator.
Described photoelectricity vacuum tube is a photomultiplier.
Also comprise the PC that links to each other with described microprocessor by the RS232 interface.
Described radiating light source is the radiating light source of single light or one group of mixed spectra.
Described radiating light source is two kinds or two kinds of semiconductor light-emitting-diode LED or semiconductor laser LD that spectrum is above.
Described semiconductor light-emitting-diode is resonant cavity light emitting diodes RCLED.
The wavelength coverage of described radiating light source is meant 400～1200nm.
Described cuvette is the liquid cuvette.
Beneficial effect of the present invention:
Absorbing light of the present invention and fluorescence spectrum composite detector are owing to adopt the automatic gather data of PC energy, demonstration, a plurality of reference mark of feedback, the modulating pulse time that has operating window, send work order by having the microprocessor that digital signal focuses on function, control has selects multiple burst length, strobe pulse to drive the wavelength and the energy of radiating light source, makes fluorescent optical sensor, absorbing light sensor be in holding state.When reaching preset condition, pulse driving circuit will optionally make a radiating light source having more than two among multi-wavelength and intensity adjustable semiconductor light-emitting-diode LED or the semiconductor laser LD export the light signal of specific wavelength and light radiation intensity in the time in certain pulses.Absorbing light sensor and fluorescent optical sensor will carry out opto-electronic conversion from absorbing light and the fluorescence that measured object in cuvette or the liquid cuvette produces, and in microprocessor, electric signal is amplified, analog signal conversion is become digital signal, give PC with digital data transmission again, by detecting requirement, focus on, show by PC again and preserve or send type information.
Therefore the present invention can be simultaneously and the multiple measured object in the synchronous detection sample; The detection of absorbing light and fluorescence spectrum is united two into one, and can obtain the absorbance and the fluorescence of multiple measured object simultaneously; By computer-controllable system and the whole testing process of analysis; The wavelength of radiating light source can be selected arbitrarily in the wavelength coverage of appointment.
The present invention not only can use separately, and can be provided on the high flux medical diagnostic equipment or in the large-scale automatic analysis system.
Description of drawings
Fig. 1, two or more radiating light source produce the time interval and the light intensity synoptic diagram of rect.p. light signal.
The fundamental diagram of Fig. 2, absorbing light and fluorescence compound detection.
The fundamental diagram that Fig. 3, absorbing light are measured.
Fig. 4, fluorimetric fundamental diagram.
Fig. 5, one embodiment of the invention.
The logic diagram of Fig. 6, absorbing light and fluorescence composite detector.
Embodiment
With reference to the accompanying drawings 6, absorbing light and fluorescence composite detector are made up of pulse driving circuit, radiating light source, cuvette, absorbing light sensor assembly, fluorescent optical sensor module, microprocessor and PC.
Pulse driving circuit is the drive source circuit of radiating light source, realizes the wavelength of multiple burst length, impulse radiation light source and the selection of energy.
Radiating light source is made up of semiconductor light-emitting-diode LED or laser instrument LD, under the pulsed drive instruction, can select and export the light signal of specific wavelength and light intensity.
The absorbing light sensor assembly is made up of absorbing light sensor PiN photodiode and amplifying circuit, can measure multiple absorbing light according to the difference in burst length.
The fluorescent optical sensor module is made up of fluorescent optical sensor PiN photodiode and amplifying circuit, can measure multiple fluorometric assay according to the difference in burst length.And the absorbing light sensor becomes 90 degree with fluorescent optical sensor, as shown in Figure 2.
Microprocessor is set the interval and the control light intensity in burst length according to detecting requirement to radiating light source; In absorbing light and fluorometric assay process, select by separating automatically of carrying out sensor time switch and absorbing light and fluorescence time of setting " window ", driving, compound and multi-wavelength control synchronously; Test data is handled, and be sent to analytical test result and demonstration test data on the PC.
PC can setting and action button and send work order; The interval that microprocessor sends the burst length as required, the wavelength and the light intensity of radiating light source are set; Control, analyze whole testing process, 3-D display and storage measured object and handle testing result; Realize interface with microprocessor.
In the process of absorbing light and the synchronous compound detection of fluorescence, radiating light source is launched the absorption light source of the light of selected wavelength as measured object, and the light that should select wavelength simultaneously also is the light source that excites fluorescent dye in the measured object.Wherein the light source of the first selected wavelength is corresponding to the absorption spectrum of first kind of measured object; Second selectes the absorption spectrum of the light of wavelength corresponding to second kind of measured object, and by that analogy.The light source activation of the first selected wavelength goes out the fluorescence spectrum corresponding to first kind of measured object, and the light source activation of the second selected wavelength goes out the fluorescence spectrum corresponding to second kind of measured object, and by that analogy.Radiating light source is subjected to time interval of the rect.p. light that pulse driving circuit control produces and light intensity as shown in Figure 1.Wherein, λ
1Be the radiating light source of the first selected wavelength, λ
2It is the radiating light source of the second selected wavelength; t
1Be the time that the first selected wavelength radiation light pulse is measured absorbing light, t
2Be that the first selected wavelength radiation light pulse is to fluorimetric time span; t
3Be the time span that the second selected wavelength radiation light pulse is measured absorbing light, t
4Be that the second selected wavelength radiation light pulse is to fluorimetric time span.
The transmitted light that the absorbing light sensor receives measured object is an absorbing light, and synchronous with the pulse interval of corresponding radiating light source.The exciting light that the light sensor of fluorescence connects measured object is a fluorescence, and synchronous with the pulse interval of corresponding radiating light source.The principle of work that Fig. 1 absorbing light is measured: shown in Fig. 3,5, when the first selected long wavelength laser or the luminescent device that is equal to are launched the two kinds of different-energies and the pulsed light beam in the time interval, wherein, by focus lamp 1 (: 10mm, f:25mm) pulse 1 (energy is lower) light is focused into a branch of radiant light transmission and crosses measured object in the cuvette 4, (: 15mm f:20mm) is focused into absorbing light one light beam and passes on the absorbing light sensor PiN photodiode D4 by focus lamp 2 again.Wherein: λ
1Be the light intensity of the first selected wavelength radiation light transmission sample, λ
2It is the light intensity of the second selected wavelength radiation light transmission sample; t
1' be the time span that the first selected wavelength radiation light pulse is measured absorbing light, t2 ' is the time span that the second selected wavelength radiation light pulse is measured absorbing light.Fluorimetric principle of work: shown in Fig. 4,5, when the first selected long wavelength laser or the luminescent device that is equal to are launched the two kinds of different-energies and the pulsed light beam in the time interval, wherein, by focus lamp 1 ( 10mm, f:25mm) pulse 2 (energy is higher) light being focused into a branch of radiant light excites the fluorescence coloring agent in the measured object in the cuvette 4 to produce fluorescence, (: 15mm f:20mm) is focused into fluorescence one light beam and passes on the fluorescent optical sensor photomultiplier D5 by focus lamp 3 again.Wherein: λ
1' be the fluorescence intensity that the first selected wavelength radiation optical excitation goes out sample, λ
2' be the fluorescence intensity that the second selected wavelength radiation optical excitation goes out sample; t
2' be the fluorescent pulse time that the first selected wavelength radiation optical excitation goes out sample, t
4' be the fluorescent pulse time that the second selected wavelength radiation optical excitation goes out sample.
According to above-mentioned absorbing light, fluorimetric principle of work, the laser instrument (or the luminescent device that is equal to) of the second selected wavelength is launched two kinds of different-energies and time interval pulsed light beam equally, and the absorbing light and the fluorescence of another measured object is detected.Can control equally the 3rd, the 4th selected long wavelength laser and to reach other measured object in the test sample.
In testing process, output need be modulated and control to the energy of radiant light can by actual detected by PC, microprocessor.The energy that detects the required radiant light of absorbing light generally is 1/3rd of a fluoroscopic examination radiant light energy.Such as, very first time rect.p. radiant light is as the usefulness of absorbing light detection, and the second time rect.p. radiant light is as the usefulness of fluoroscopic examination.
If as spectral analysis, can pass through the light resolution element of wave filter, grating or monochromator or similar functions, absorbing light and fluorescence spectrum are accumulated on the photoelectricity coupling array sensor, for example, photoelectricity coupled matrix CCD, complementary matal-oxide semiconductor sensor CMOS can obtain more accurate spectral intensity distribution plan.
Because the absorbing light and the fluorescence spectrum of each measured object are known, sample produces absorbing light under the radiant light effect and fluorescence spectrum can carry out the wave spectrum contrast and these data store and a pure reference sample at first is used as, so in the sample detection process, on known data channel, can judge the feature peak shape at measured object mixing peak to the shape of measured object absorbing light and fluorescence spectrum, and can carry out qualitative, Comparative and Quantitative Analysis to this peak shape with corresponding software.In this course, photoelectricity coupling array device can only be collected narrow wavelength coverage in one section corresponding measured object absorbing light and the fluorometric assay, as the wavelength coverage of 400～1200nm.To this application, can select high density, quick light sensor.As mixed electrical optical coupling square row CMOS-CCD, photodiode arrangement PDA.On the basis of same computer clock benchmark, optical sensor and radiating light source are finished optical signal transmitting and reception by microprocessor, and are connected with computing machine, reach whole process automation and control and finish.
Also can select single light sensor, replace photoelectricity coupled matrix CCD as photomultiplier or photodiode, cost is lower, and it is easier to operate.
The present invention both can adopt common cuvette also can adopt the liquid cuvette; Can need both can be used as according to actual detected and carry out light absorbing detection or fluoroscopic examination separately, also can the two simultaneously, carry out synchronously.
Recombination radiation light source control of the present invention and light signal read an embodiment, and as shown in Figure 5, field effect transistor U9, U10 and the U11 of microprocessor U1 command N passage open light emitting diode D1, D2 and D3.Microprocessor is controlled to the light emitting diode D1, the D2 that open and the maximum current of D3, and passes through the field effect transistor U5 of N passage, and U6, U7 and U8 realize " open-minded " and " disconnection ".The resistance value of resistance R 1, R2, R3, R4 and R5 depends on to the maximum current of opening light emitting diode D1, D2 and D3.Four data output ports connect resistance R 2, R3, R4 and R5 among the microprocessor U1.Therefore, total system can be set 16 layers of different radiant light strength control.
In the burst length of the light emitting diode D1, the D2 that open or D3, the modulus word of microprocessor U1 conversion A/D receives from the simulating signal of absorbing light amplifier U3 or fluorescence amplifier U4 and these are signal digitalized.Microprocessor U1 transmits all digital signals by communication port RS232 and gives PC, and receives the steering order from PC control light emitting diode D1, D2 and D3 size of current, pulse interval, amplifier U3 or U4 gain.
Various variations or the remodeling made by those skilled in the art can not depart from the scope of the present invention.
Claims (14)
1, absorbing light and fluorescence spectrum composite detector, comprise radiating light source, cuvette and microprocessor, it is characterized in that: also comprise position 90 absorbing light sensor assembly and the fluorescent optical sensor modules of spending and linking to each other each other with described microprocessor, described microprocessor links to each other with described radiating light source by pulse driving circuit, is respectively equipped with focus lamp between described cuvette and described radiating light source, absorbing light sensor assembly and the fluorescent optical sensor module;
Described microprocessor is used to set and export the pulse interval that produces absorbing light, fluorescence respectively and the steering order of pulse strength;
Described pulse driving circuit is used for the steering order according to described microprocessor, makes described radiating light source select different optical wavelength and light intensity output in the different burst lengths;
Described absorbing light sensor assembly comprises the amplifying circuit that links to each other with the absorbing light sensor, is used for the steering order according to described microprocessor, becomes electric signal to output to described microprocessor the absorbing light conversion of signals in the light absorbing burst length;
Described fluorescent optical sensor module comprises the amplifying circuit that links to each other with fluorescent optical sensor, is used for the steering order according to described microprocessor, converts fluorescence signal to electric signal and output to described microprocessor in the burst length of fluorescence.
2, absorbing light according to claim 1 and fluorescence spectrum composite detector is characterized in that: described pulse driving circuit comprises ON-OFF control circuit and current control circuit; The input end of field effect transistor links to each other with described microprocessor in the described ON-OFF control circuit, and output terminal links to each other with described radiating light source; Described current control circuit is made of the field effect transistor and the peripheral circuit thereof that link to each other with described microprocessor.
3, absorbing light according to claim 1 and fluorescence spectrum composite detector is characterized in that: be provided with light separator between described focus lamp and described absorbing light sensor.
4, absorbing light according to claim 1 and fluorescence spectrum composite detector is characterized in that: be provided with light separator between described focus lamp and described fluorescent optical sensor.
5, absorbing light according to claim 1 and fluorescence spectrum composite detector is characterized in that: described absorbing light sensor is PiN photodiode, photoelectricity coupling array sensor, photodiode arrangement or photoelectricity vacuum tube.
6, according to described absorbing light of claim 1 and fluorescence spectrum composite detector, it is characterized in that: described fluorescent optical sensor is PiN photodiode, photoelectricity coupling array sensor, photodiode arrangement or photoelectricity vacuum tube.
7, according to claim 3 or 4 described absorbing light and fluorescence spectrum composite detectors, it is characterized in that: described light separator is wave filter, grating or monochromator.
8, according to claim 5 or 6 described absorbing light and fluorescence spectrum composite detectors, it is characterized in that: described photoelectricity vacuum tube is a photomultiplier.
9, according to described absorbing light of one of claim 1～4 and fluorescence spectrum composite detector, it is characterized in that: also comprise the PC that links to each other with described microprocessor by the RS232 interface.
10, according to described absorbing light of one of claim 1～4 and fluorescence spectrum composite detector, it is characterized in that: described radiating light source is the radiating light source of single light or one group of mixed spectra.
11, absorbing light according to claim 10 and fluorescence spectrum composite detector is characterized in that: described radiating light source is two kinds or two kinds of semiconductor light-emitting-diode or semiconductor lasers that spectrum is above.
12, absorbing light according to claim 11 and fluorescence spectrum composite detector is characterized in that: described semiconductor light-emitting-diode is a resonant cavity light emitting diodes.
13, absorbing light according to claim 10 and fluorescence spectrum composite detector is characterized in that: the wavelength coverage of described radiating light source is meant 400～1200nm.
14, according to described absorbing light of one of claim 1～4 and fluorescence spectrum composite detector, it is characterized in that: described cuvette is the liquid cuvette.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102128799A (en) * | 2010-12-21 | 2011-07-20 | 无锡荣兴科技有限公司 | Water quality detection sensor |
| CN101706434B (en) * | 2009-09-04 | 2011-08-10 | 中国环境科学研究院 | Fluorophototmeter capable of detecting light absorption or light transmission |
| CN102519881A (en) * | 2011-12-29 | 2012-06-27 | 北京国科华仪科技有限公司 | Optical detection system applicable to absorbed light detection and fluorescence detection |
| CN103649726A (en) * | 2011-03-08 | 2014-03-19 | 堀场仪器株式会社 | System and method for fluorescence and absorbance analysis |
| CN106996913A (en) * | 2016-01-25 | 2017-08-01 | 苑高强 | A kind of material identifier and Internet of Things |
| CN109642870A (en) * | 2016-09-01 | 2019-04-16 | 亚琛工业大学 | Method and apparatus for detecting the procedure parameter in liquid culture |
-
2008
- 2008-01-18 CN CN200810043038A patent/CN100588950C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101706434B (en) * | 2009-09-04 | 2011-08-10 | 中国环境科学研究院 | Fluorophototmeter capable of detecting light absorption or light transmission |
| CN102128799A (en) * | 2010-12-21 | 2011-07-20 | 无锡荣兴科技有限公司 | Water quality detection sensor |
| CN103649726A (en) * | 2011-03-08 | 2014-03-19 | 堀场仪器株式会社 | System and method for fluorescence and absorbance analysis |
| CN103649726B (en) * | 2011-03-08 | 2016-08-17 | 堀场仪器株式会社 | For fluorescence and the system and method for absorptance analysis |
| CN102519881A (en) * | 2011-12-29 | 2012-06-27 | 北京国科华仪科技有限公司 | Optical detection system applicable to absorbed light detection and fluorescence detection |
| CN106996913A (en) * | 2016-01-25 | 2017-08-01 | 苑高强 | A kind of material identifier and Internet of Things |
| CN106996913B (en) * | 2016-01-25 | 2020-11-10 | 苑高强 | Material identifier and Internet of things |
| CN109642870A (en) * | 2016-09-01 | 2019-04-16 | 亚琛工业大学 | Method and apparatus for detecting the procedure parameter in liquid culture |
| US11635381B2 (en) | 2016-09-01 | 2023-04-25 | Rheinisch-Westfälisch Technische Hochschule (RWTH) Aachen | Method and device for measuring process parameters in liquid cultures |
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| CN100588950C (en) | 2010-02-10 |
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Free format text: CORRECT: ADDRESS; FROM: 201203 PUDONG NEW AREA, SHANGHAI TO: 200063 PUTUO, SHANGHAI |
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Effective date of registration: 20120305 Address after: 200063, room 320, 505 West Langao Road, Shanghai, Putuo District Patentee after: Hu Sijun Address before: 201203 C411 room, No. 328 blue wave road, Zhangjiang hi tech Development Zone, Shanghai, China Patentee before: Shanghai Hengdao Photoelectric Instrument Co., Ltd. |
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Granted publication date: 20100210 Termination date: 20200118 |