CN201368879Y - Biological test sensor - Google Patents

Abstract

The utility model relates to a biological detection sensor, which is characterized in that it includes a mounting frame, a cantilever mechanism, a test tray, a peristaltic pump and a single-chip microcomputer; A guide rail is arranged horizontally on the beam; the cantilever mechanism includes a linear stepping motor and a photoelectric conversion component whose output end is connected to a needle; The test tray is a rectangular frame with four sides closed, which is set on the horizontal support plate under the cantilever mechanism; a liquid level detection window with a liquid level detection switch is opened on the horizontal support plate on one side of the test tray; the peristaltic The pump is connected with a pipeline, and the outlet end of the pipeline is connected with the needle; the single-chip microcomputer is connected with the input ends of each stepping motor and electromagnet. The utility model realizes the automation of biological inspection, and can be widely used in on-line monitoring in the fields of environmental protection, medical diagnosis, food and drug production, and the like.

Description

A biometric sensor

technical field

The utility model relates to a sensor, in particular to a fully automatic biological detection sensor used to detect whether a sample liquid contains toxic components.

Background technique

Immunochromatography (Immunochromatography) is a rapid diagnostic technique that has emerged in recent years. Its principle is to immobilize specific antibodies on a certain zone of the nitrocellulose membrane, and when one end of the dry nitrocellulose membrane is immersed in the sample, , due to capillary action, the sample moves forward along the membrane. When it moves to the area where the antibody is immobilized, the corresponding antigen in the sample is specifically combined with the antibody. If immunocolloidal gold or immunoenzyme staining can make the The area displays a certain color, thus realizing specific immunodiagnosis.

Immunogold labeling technique utilizes the characteristics of high electron density of gold particles. When the gold-labeled protein aggregates in a large amount at the corresponding ligand, it can form red or pink spots visible to the naked eye, so it is used for qualitative or semi-quantitative rapid immunoassay method. The basic principle is to coat the antigen on the detection line (T) of the nitrocellulose membrane, coat the synthetic antibody on the control line (C) of the nitrocellulose membrane, dry the antigen labeled with colloidal gold and place it in the Lower end of nitrocellulose membrane. Put the sample into the sample hole, due to the principle of chromatography, the sample to be tested begins to move upward along the paper strip, when it encounters the dry gold-labeled antigen, dissolves it, and continues to move upward with the gold-labeled antigen until it reaches the nitric acid Detection line for cellulose membrane. If there is an antibody in the sample, the antigen coated on the detection line, the gold-labeled antigen and the antibody in the sample will form a purple-red sandwich immune complex on the detection line. The detection line is located at the lower end of the control line. To reach the control line, the sample must first pass through the detection line. When both the detection line and the control line appear, it can be judged as positive; if the detection line does not appear, only the control line can be judged as positive. Negative.

In recent years, colloidal gold-labeled immunochromatographic test paper detection technology has been widely used in medical diagnosis, biological and chemical testing and other fields due to its simplicity, convenience, rapid response, and easy identification. This detection method is suitable for both naked eye visual judgment and instrument identification, but visual judgment has the disadvantages that it cannot be measured quantitatively, but can only be measured qualitatively; the objectivity of the measurement results is poor; and the dynamic process of biological reactions cannot be monitored. In order to overcome these shortcomings, some detection instruments have been developed successively in China, such as: the publication number published on October 29, 2003 is CN1451963A, and the name of the utility model patent is: gold standard test strip quantitative testing method and system; February 15, 2006 The authorized authorization announcement number is CN2758754Y, and the utility model patent name is: gold standard test strip optical fiber probe device; the publication number published on January 31, 2007 is CN1904592A, and the utility model patent name is: reflection type of gold standard immune test strip Photometer; the publication number disclosed on November 28, 2007 is CN101078722A, and the utility model patent name is: a gold standard reagent quantitative detection device and measurement method. The development of these instruments overcomes some shortcomings of artificial visual judgment, improves the detection level, and is conducive to the standardization of recognition judgment. However, in addition to the result identification and judgment, other operations of the above instruments are done manually, and only one sample can be tested at a time, and it is impossible to realize online automatic work and simultaneous detection of multiple samples.

Contents of the invention

In view of the above problems, the purpose of this utility model is to propose a fully automatic biological detection sensor for detecting whether the sample liquid contains toxic components.

In order to achieve the above object, the utility model adopts the following technical solutions: a biological detection sensor, which is characterized in that: it includes a mounting frame, a cantilever mechanism, a test disk warehouse, a peristaltic pump and a single-chip microcomputer; the mounting frame consists of It consists of two side plates, a rear side plate, a horizontal support plate and a support beam, and at least one X-axis guide rail is horizontally arranged on the support beam; the cantilever mechanism includes a vertically arranged Z-axis stepping motor and a The light source and photoelectric conversion components fixed on one side of the Z-axis stepping motor, wherein the Z-axis stepping motor is a linear stepping motor, and its output end is connected to a needle; the cantilever mechanism is slidably arranged on the X axis guide rail, and the cantilever mechanism is connected to a transmission belt that drives it to move linearly on the X-axis guide rail, the transmission belt is connected to the output end of an X-axis stepping motor, and the X-axis stepping motor is fixed On the rear side plate; the rear side plate at one end of the transmission belt is provided with an X-axis limit switch connected with the X-axis stepping motor; the cantilever mechanism is also provided with a The Z-axis limit switch connected to the Z-axis stepping motor; the test disk warehouse is a rectangular frame with four sides closed, which is arranged on the horizontal support plate below the cantilever mechanism; the test disk warehouse on one side A circular liquid level detection window is provided on the horizontal support plate, and a liquid level detection switch is arranged in the liquid level detection window; the peristaltic pump is fixed on the rear side plate at the lower part of the horizontal support plate, and is connected to A pipeline, the inlet end of the pipeline is connected to the external liquid inlet, and the outlet end is connected to the needle; the single-chip microcomputer is fixed on the rear side plate, which is connected to the input of each stepper motor and electromagnet terminal, and the single-chip microcomputer is equipped with a communication serial port for connecting with the host computer.

The test tray includes a rectangular cavity that runs through the horizontal support plate and a tray that is movably arranged in the rectangular cavity; the front and rear sides of the test tray are respectively provided with a vertical plate, and the vertical plate Both ends have an extension arm in the vertical direction, a hook is provided on the top of the extension arm, and the four hooks are connected to the tray through four springs; an empty detection device is arranged on the vertical plate on one side. For the switch, four large electromagnets and four guide wheels are symmetrically arranged on the horizontal support plates outside the two vertical plates, and the telescopic end of each of the large electromagnets is connected to a wedge-shaped part, which is in line with the position of the wedge-shaped part. There are four square holes on the corresponding vertical plate; two small electromagnets are arranged symmetrically front and back on the right side of the test tray, and the telescopic ends of the small electromagnets are connected to a baffle.

A push disk mechanism is arranged on the left side of the test disk chamber, the push disk mechanism includes a push disk, and the outside of the push disk is connected with a push disk shaft horizontally arranged above the horizontal support plate The other end of the push plate connecting rod is connected to the upper end of the push plate connecting rod vertically passing through the horizontal support plate, and the lower end of the push plate connecting rod is connected to a horizontally arranged rack. The push plate shaft guide rail of the rack, the push plate shaft guide rail is fixed on the two side plates; a push plate stepper motor connected with the rack is fixed on the push plate shaft guide rail, and the push plate shaft guide rail is fixed on the push plate shaft guide rail. A push plate limit switch connected with the push plate stepping motor is arranged on the outer side of the plate connecting rod.

It also includes a test disc, the test disc is a rectangular disc body matching the shape of the test disc chamber, and several rectangular test paper slots for placing test strips are arranged on the test disc, each of which The bottom of the test paper tank is provided with a circular liquid injection hole connected thereto; the front and rear sides of the test disc are provided with four wedge-shaped slots that match the wedge-shaped parts of the large electromagnet; The upper surface is covered with a protective film.

A waste liquid box for collecting waste liquid is arranged below the liquid level detection window.

The X-axis limit switch, the Z-axis limit switch and the liquid level detection switch adopt a photoelectric transmitting and receiving pair tube, and the described empty detection switch and the push plate axis limit switch adopt a magnetic switch.

Because the utility model adopts the above technical scheme, it has the following advantages: 1. The pipeline connected with the needle in the utility model can be connected with the relevant sampling instrument or sample container, and the sample liquid is automatically extracted by the peristaltic pump, and the liquid pipeline is automatically discharged The air inside, the cantilever mechanism drives the needle to move horizontally and vertically, and the automatic quantitative injection of the needle is accurately realized. 2. The light source and the photoelectric conversion part of the present invention convert the grayscale signal of the test strip into a voltage signal, and input it to the AD input terminal of the single-chip microcomputer, and the AD converter in the single-chip microcomputer converts the analog voltage signal into a digital signal, and then It is transmitted to the upper computer through the communication serial port, and the detection result is displayed in the upper computer. The whole detection process does not require manual operation. 3. The utility model integrates a plurality of detection test strips on one test disc, and realizes simultaneous detection of multiple samples. 4. The utility model can automatically discharge and collect the remaining sample liquid in the waste liquid box through the peristaltic pump, and can automatically flush the liquid pipeline. 5. The utility model is equipped with a push plate mechanism on one side of the test plate chamber, and the push plate mechanism can automatically replace the test plate after the test is completed, so as to prepare for the next test. 6. The utility model can not only be controlled on site, but also can realize remote control and detection through the connection of RS485/422, etc., which reduces the labor intensity of the operator and improves the safety of the operator. In summary, the utility model realizes the automation of biological inspection, and can be widely used in on-line monitoring in the fields of environmental protection, medical diagnosis, and food and drug production.

Description of drawings

Fig. 1 is the three-dimensional structure schematic diagram of the present utility model

Fig. 2 is the front view of the utility model

Figure 3 is a top view of Figure 2

Fig. 4 is the structural representation of the test disc of the present utility model

Figure 5 is a schematic diagram of the work flow when the utility model pushes the plate

Detailed ways

Below in conjunction with accompanying drawing and embodiment the utility model is described in detail.

As shown in Fig. 1～Fig. 3, the utility model comprises a mounting frame 1, a cantilever mechanism 2, a test disc storehouse 3, a push plate mechanism 4, a peristaltic pump 5 and a single-chip microcomputer (not shown). The mounting frame 1 is made up of two side plates 11, a rear side plate 12, a transverse support plate 13 and a support beam 14, and two X-axis guide rails 6 are horizontally arranged on the support beam 14 (this is only an example, not limited to this).

The cantilever mechanism 2 includes a vertically arranged Z-axis stepping motor 21 and a light source and a photoelectric conversion component 22 fixed on the right side of the Z-axis stepping motor 21, wherein the Z-axis stepping motor 21 is a linear stepping motor, and its output The end is connected with a needle head 23, and the needle head 23 moves linearly in the vertical direction under the drive of the Z-axis stepping motor 21. The cantilever mechanism 2 is slidably arranged on the two X-axis guide rails 6, and the cantilever mechanism 2 is connected to a transmission belt 24 that drives it to move linearly on the X-axis guide rails 6, and the transmission belt 24 is connected to the output end of an X-axis stepping motor 25, The X-axis stepping motor 25 is fixed on the rear side plate 12 . The rear side plate 12 at one end of the drive belt 24 is provided with an X-axis limit switch 26 connected to the X-axis stepping motor 25 to determine the zero position of the cantilever mechanism 2 on the X-axis guide rail 6 . The cantilever mechanism 2 is also provided with a Z-axis limit switch 27 connected to the Z-axis stepping motor 21 to determine the zero position of the needle 23 on the cantilever mechanism 2 .

The test tray 3 is arranged on the horizontal support plate 13 below the cantilever mechanism 2 , and includes a rectangular cavity 301 passing through the horizontal support plate 13 and a tray 302 movably arranged in the rectangular cavity 301 . The front and rear sides of the test tray 3 are respectively provided with a vertical plate 303, and the two ends of the vertical plate 303 respectively have an extension arm 304 in the vertical direction, and a hook 305 is arranged on the top of the extension arm 304, and the four hooks 305 pass through four The spring 306 is connected to the tray 302 so that the tray 302 can move up and down in the rectangular cavity 301 . An empty detection switch 307 is arranged on one side vertical board 303, and four large electromagnets 308 and four guide wheels 309 are symmetrically arranged on the horizontal support plate 13 outside the two vertical boards 303, and the expansion and contraction of each large electromagnet 308 The end is connected with a wedge-shaped part 310, and four square holes are opened on the vertical plate 303 corresponding to the position of the wedge-shaped part 310. Two small electromagnets 311 are arranged symmetrically front and rear on the right side of the test tray 3 , and the telescopic ends of the small electromagnets 311 are connected to a baffle 312 . A circular liquid level detection window 7 is provided on the horizontal support plate 13 on the left side of the test tray chamber 3 , and a liquid level detection switch 71 is arranged in the liquid level detection window 7 . A waste liquid box 8 is arranged below the liquid level detection window 7 for collecting used waste liquid.

The push plate mechanism 4 includes a push plate 41 arranged on the left side of the test disc chamber 3, the outside of the push plate 41 is connected with a push plate shaft 42 horizontally arranged above the horizontal support plate 13, and the other end of the push plate shaft 42 is connected vertically through The upper end of the push plate connecting rod 43 of the horizontal support plate 13, the lower end of the push plate connecting rod 43 is connected to a horizontally arranged rack 44, and a push plate shaft guide rail for supporting and fixing the rack 44 is horizontally arranged below the rack 44 45, the push plate shaft guide rail 45 is fixed on the side plates 11. A push plate stepper motor 46 connected with the rack 44 is fixed on the push plate shaft guide rail 45 and the push plate stepper motor 46 drives the push plate 41 by the push plate mechanism 4 to do horizontal linear motion in the test disc storehouse 3 . The outer side of the push plate connecting rod 43 is provided with a push plate limit switch 47 connected with the push plate stepper motor 46 to limit the stroke of the push plate 41 .

The peristaltic pump 5 is fixed on the rear side plate 12 at the bottom of the cross support plate 13 and is connected to a pipeline 51 , the inlet of the pipeline 51 is connected to the external liquid inlet, and the outlet is connected with the needle 23 .

Single-chip microcomputer is fixed on the rear side plate 12, and it connects the input end of each stepper motor and electromagnet, controls the motion of each stepper motor and electromagnet. The single-chip microcomputer is also equipped with a communication serial port, which can be connected with an upper computer (such as a PC), and input the detection results and data into the upper computer.

As shown in Figure 4, the utility model also includes a test disc 9 that matches the shape of the test disc chamber 3, and the test disc 9 is provided with several rectangular test paper slots 91 for placing test strips, each test paper slot 91 The lower part of each is provided with a circular liquid injection hole 92 communicating therewith. There are four wedge-shaped slots 93 matched with the wedge-shaped part 310 of the large electromagnet 308 on the front and rear sides of the test disc 9 . The upper surface of the test disc 9 is covered with a protective film to prevent the test strips from getting wet and being polluted by dust. The test discs 9 are placed on the tray 302 in advance, and more than ten discs can be put in at a time.

In the above embodiment, the X-axis limit switch 26, the Z-axis limit switch 27 and the liquid level detection switch 71 adopt the photoelectric transmitting and receiving pair tube, and the disk empty detection switch 307 and the push plate axis limit switch 48 adopt the magnetic switch .

In the above embodiment, if only one test disk 9 needs to be tested each time, the test disk chamber 3 can be simplified into a rectangular slot, and the push disk mechanism 4 can also be omitted.

After the utility model is powered on, firstly, each motor will be returned to the zero position, and it will enter an idle state, waiting to receive instructions from the upper computer. After receiving the starting command, the workflow of the utility model is divided into the following six stages:

1. Filling pipeline: the X-axis stepping motor 25 drives the cantilever mechanism 2 to move horizontally, and stops when it is directly above the liquid level detection window 7; then, the Z-axis stepping motor 21 drives the needle 23 to move downwards to the liquid level Stop after detecting the vicinity of switch 71; Then, peristaltic pump 5 begins to draw sample liquid, and liquid will begin to fill pipeline 51 from pipeline 51 inlet end, and after liquid reaches needle 23, will drip from needle point; Via the liquid level detection switch 71, the liquid level detection switch 71 will be triggered, and then the Z-axis stepper motor 21 will move upward to the zero position.

2. Inject the sample: the X-axis stepping motor 25 drives the cantilever mechanism 2 to move to the top of the first liquid injection hole 92 on the test plate 9 and then stops, and then the Z-axis stepping motor 21 drives the needle 23 to move downwards, so that The needle tip pierces the protective film on the test disc 9 and is inserted into the liquid injection hole 92 . Next, the peristaltic pump 5 rotates at a slow speed, injects about a few drops of sample liquid into the liquid injection hole 92 and then stops; then the needle 23 returns upward to the zero position. In this way, the liquid injection of one liquid injection hole 92 is completed. The remaining twelve liquid injection holes 92 are all performed with the same steps. After injecting all thirteen injection holes 92, the X-axis stepper motor 25 returns to the zero position.

3. Infiltration: This period of time is the infiltration time of the sample liquid and the test strip, generally about five minutes.

4. Scanning: Scanning is to use the light source and the photoelectric conversion part 22 to read the stripes of the test strip on the test disc 9 and upload it to the host computer. The signal is converted into a voltage signal. After a series of operations such as amplification and filtering, the weak voltage signal is input to the AD input terminal of the single-chip microcomputer. The AD converter in the single-chip microcomputer converts the analog voltage signal into a digital signal, and then the data is simply processed. After that, it is transmitted to the host computer through the communication serial port. This process is a dynamic process and lasts for about ten minutes, so the data should be read continuously during this time. The light source and the photoelectric conversion unit 22 need to carry out ten reading cycles altogether, and each reading cycle will read the thirteen test strips once. The X-axis stepping motor 25 drives the light source and the photoelectric conversion component 22 to move to the position of the test strip, and starts the light source and the photoelectric conversion component 22 to start reading data. In order to eliminate the alignment error between the lens and the test strips, the X-axis stepping motor 25 will move slightly and read data multiple times near each test strip.

5. Change the test disk: each test disk 9 can only be used once, when the test disk 9 is used up, it will be pushed to the waste disk cylinder, and the new disk below will be automatically moved up for the next use. When pushing the disc, the disc stepper motor 46 cooperates with each electromagnet to complete the whole operation. The specific process is as follows (as shown in Figure 5): at first, four large electromagnets 308 act, and the wedge-shaped part 310 and the test disc 9 The wedge-shaped groove 93 cooperates to generate downward force, and the test disk 9 on the top is separated from the test disk 9 below; 311 action, the baffle 312 of the small electromagnet 311 and the other three sides form a four-sided closed frame to ensure that the upwardly moving test disc 9 will not be displaced; then, the large electromagnet 308 loses power, and the lower test disc 9 fills up ; Finally, the small electromagnet 311 is de-energized, and the entire pushing operation is completed.

6. Cleaning the pipeline: after the test, the sample liquid is discharged to the waste liquid box 8 for re-test or evacuation. The remaining sample liquid in the pipeline 51 will affect the next use. Therefore, after the sample liquid is emptied, fill the cleaning solution into the pipeline 51, and discharge it to the waste liquid box 8 through the peristaltic pump 5. After three times, close the liquid circuit valve. , the utility model returns to the standby state.

In summary, the utility model can realize the automation of biological inspection, and can also realize remote control and detection through the connection of RS485/422, etc., providing a safe and convenient way for pathogen detection.

The utility model is only described with the above-mentioned embodiment, and the structure, setting position, and connection of each component can be changed. Improvements and equivalent transformations should not be excluded from the protection scope of the present utility model.

Claims (9)

1, a kind of bioassay sensor is characterized in that: it comprises an erecting frame, a cantilever mechanism, a test plate bin, a peristaltic pump and a single-chip microcomputer; Described erecting frame is made up of biside plate, a back side panel, a horizontal support plate and a brace summer, has been horizontally disposed with at least one X-axis guide rail on the described brace summer;

Described cantilever mechanism comprises that a vertically disposed Z shaft step motor and is fixed on the light source and the photoelectric conversion part of described Z shaft step motor one side, and wherein said Z shaft step motor is a straight line stepper motor, and its output terminal connects a syringe needle; Described cantilever mechanism is slidingly arranged on the described X-axis guide rail, and described cantilever mechanism connects a driving, and it makees straight-line driving belt on described X-axis guide rail, described driving belt connects the output terminal of an X-axis stepper motor, and described X-axis stepper motor is fixed on the described back side panel; The described back side panel of described driving belt one end is provided with an X-axis limit switch that is connected with described X-axis stepper motor; Also be provided with a Z axle limit switch that is connected with described Z shaft step motor on the described cantilever mechanism;

Described test plate bin is the closed rectangle framework in a four sides, and it is arranged on the horizontal support plate of cantilever mechanism below; Offer the Level Detection window of a circle on the described horizontal support plate of described test plate bin one side, be provided with a Level Detection switch in the described Level Detection window;

Described peristaltic pump is fixed on the described back side panel of described horizontal support plate bottom, and it connects a pipeline, and the inlet end of described pipeline connects outside place, liquid inlet, and endpiece is connected with described syringe needle;

Described single-chip microcomputer is fixed on the described back side panel, and it connects the input end of each described stepper motor and electromagnet, and is equipped with on the described single-chip microcomputer and is used for the communication serial port that is connected with host computer.

2, a kind of bioassay sensor as claimed in claim 1 is characterized in that: described test plate bin comprises that a rectangular cavities and that runs through described horizontal support plate is movably arranged on the pallet in the described rectangular cavities; The both sides, front and back of described test plate bin are respectively arranged with a riser, and the two ends of described riser respectively have an extension arm at vertical direction, and the top of described extension arm is provided with a hook, and four described hooks connect described pallet by four springs; The described riser of one side is provided with the detector switch that circles or whirl in the air, be symmetrically arranged with four big electromagnet and four guide wheels on the described horizontal support plate in the two described riser outsides, the flexible end of each described big electromagnet connects a wedge-shaped member, has four square openings on the described riser corresponding with described wedge-shaped member position; Be symmetrically arranged with two little electromagnet before and after the right side of described test plate bin, the flexible end of described little electromagnet connects a baffle plate.

3, a kind of bioassay sensor as claimed in claim 2, it is characterized in that: be provided with a push-plate mechanism in described test plate bin left side, described push-plate mechanism comprises a push-plate, described push-plate outside connection one is horizontally set on the push-plate axle of described horizontal support plate top, the other end of described push-plate axle connects the upper end of the push-plate connecting link that vertically runs through described horizontal support plate, the lower end of described push-plate connecting link connects a horizontally disposed tooth bar, the lower horizontal of described tooth bar is provided with a push-plate axis rail that is used for supporting and fixing described tooth bar, and described push-plate axis rail is fixed on the two described side plates; Be fixed with a push-plate stepper motor that is connected with described tooth bar on the described push-plate axis rail, the arranged outside of described push-plate connecting link has a push-plate limit switch that is connected with described push-plate stepper motor.

4, as claim 1 or 2 or 3 described a kind of bioassay sensors, it is characterized in that: it also comprises a test panel, described test panel is a rectangle disk body that matches with described test plate bin shape, described test panel is provided with several rectangle test paper grooves that is used for placing test-strips, and the bottom of each described test paper groove is provided with one and is attached thereto logical circular liquid injection hole; The both sides, front and back of described test panel are provided with four wedge-shaped slots that match with the wedge-shaped member of described big electromagnet; The upper surface of described test panel is coated with one deck protective film.

5, as claim 1 or 2 or 3 described a kind of bioassay sensors, it is characterized in that: the below of described Level Detection window is provided with one and is used for collecting the waste liquid box of liquid waste.

6, a kind of bioassay sensor as claimed in claim 4 is characterized in that: the below of described Level Detection window is provided with one and is used for collecting the waste liquid box of liquid waste.

7, as claim 1 or 2 or 3 described a kind of bioassay sensors, it is characterized in that: the photoemission that described X-axis limit switch, Z axle limit switch and Level Detection switch adopt receives pipe, and what described circle or whirl in the air detector switch and push-plate axle limit switch adopted is magnetic switch.

8, a kind of bioassay sensor as claimed in claim 4, it is characterized in that: the photoemission that described X-axis limit switch, Z axle limit switch and Level Detection switch adopt receives pipe, and what described circle or whirl in the air detector switch and push-plate axle limit switch adopted is magnetic switch.

9, a kind of bioassay sensor as claimed in claim 6, it is characterized in that: the photoemission that described X-axis limit switch, Z axle limit switch and Level Detection switch adopt receives pipe, and what described circle or whirl in the air detector switch and push-plate axle limit switch adopted is magnetic switch.

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