CN206540925U - A kind of linear propulsion parallel processing reaction unit of integrated reagent set - Google Patents

Abstract

The utility model discloses a kind of linear propulsion parallel processing reaction unit of integrated reagent set, including support frame, support frame both sides are provided with the linear slide being engaged, linear slide is provided with some stations along slideway direction, the integrated card holder that linear slide is combined with linear synchronous propeller is used to clamp integrated reagent card, linear synchronous propeller promotes integrated reagent card to move forward and reset along linear slide, sample filling apparatus is sequentially provided with along in linear slide, reaction solution filling apparatus, negative reaction device, negative pressure wash mill and luminous substrate liquid adding set, the integrated reagent of integrated reagent card preloaded completes sample-adding, reaction, washing and luminescent solution add operation.The utility model solves multi-index cluster and greatly shortened into the detection and report result interlude for the problem of reagent set parallel processing and reaction, making the detection project group of patient two-by-two, improves immune detection efficiency.

Description

A kind of linear propulsion parallel processing reaction unit of integrated reagent set

Technical field

The utility model discloses a kind of linear propulsion parallel processing reaction unit of integrated reagent set, particularly for examining in vitro The disconnected multi objective of detection immediately is combined the parallel processing reaction unit of project team.

Background technology

In instant detection process, parallel parsing and simultaneously report any combination multi objective result, with important reality Meaning.Multi objective can provide more, more accurately diagnostic message for doctor, and correct diagnosis is made for doctor；And any multi objective Parallel parsing is combined, the instant detection needs of different status of patient can be preferably met.During actually detected, each patient's Ill different, test event, quantity are differed；The project team that each patient is detected examines as a unit integrated project group Survey, it is necessary to have to this can synchronization process in groups device, realization synchronously completes reaction, and report result parallel for project team is When detect, these synchronization process processes are extremely important.

Denmark's thunder degree AQT90 FLEX immunity analysis instruments, France Mei Liai BioMerieux Vidas, Mitsubishi It is the more typical instant detection means of whole blood sample multi objective of a class to learn PATHFAST, particular area multi objective can be achieved parallel Analysis and report result.Other liquid components are distributed into the integrated morphology of single part by this kind of device in advance, add during without detecting Reagent adding；This kind equipment is the formal machine completion test to organize when detecting by the test index reagent of a patient；But it is this kind of Instrument need to be after this group of index test be finished, then starts project team's test of other patients.The project team of each patient surveys Examination is usually 16~20 minutes, and the 2nd patient's stand-by period to be checked is long after the 1st Patient Sample A enters detection, and efficiency is low, Full-page proof processing when being unfavorable for outpatient service etc. compared with multisample.

Luminex systems are a kind of Multiple detection reporting systems.The system uses fluidic cell cell resolution techniques, fluorescence Coding microball identification technology, immunofluorescence label reporting techniques so that primary sample can be while examining report many indexes, reason By can above reach the index of more than 100.But due to the finiteness of same patient's Testing index on Clinical practice, each index Compatibility between immunoreactant, and apparatus structure complexity, Luminex systems turn into the conventional hand of clinical examination Section.In addition, specimen in use can only be serum or blood plasma, it is impossible to use whole blood sample, be not suitable for detection immediately.

Therefore, in instant detection scheme, research be adapted to the automatic paralleling analytical technologies of limited multi objective of clinical needs according to It is so significant.

Utility model content

In view of the shortcomings of the prior art, the utility model provides a kind of linear propulsion parallel processing reaction of integrated reagent set Device, it is intended to solve multi-index cluster into the problem of reagent set parallel processing and reaction.Many fingers of the utility model to each patient Mark the integrated reagent reacting unit parallel processing in any project team and complete course of reaction, and make the detection project of patient two-by-two The detection of group and report result interlude are greatly shortened, and improve immune detection efficiency.

To reach above-mentioned purpose, the utility model is achieved through the following technical solutions：

A kind of linear propulsion parallel processing reaction unit of integrated reagent set, it includes support frame, and support frame both sides are provided with phase The linear slide of cooperation, linear slide is provided with some stations along slideway direction, and linear slide is combined with linear synchronous propeller Integrated card holder is used to clamp integrated reagent card, and linear synchronous propeller promotes integrated reagent card to be moved forward simultaneously along linear slide Reset, sample filling apparatus, reaction solution filling apparatus, negative reaction device, negative pressure wash mill are sequentially provided with along in linear slide With luminous substrate liquid adding set, the integrated reagent of integrated reagent card preloaded completes sample-adding, reaction, washing and luminescent solution addition Operation.

Further, at least one group integrated reagent card concurrent job in linear slide, each integrated reagent card group is by linear same Step propeller control synchronously moves forward along linear slide, the integrated reagent card of integrated card gripping means grips, linear synchronizing propeller Integrated card holder and integrated reagent card is promoted integrally to advance a station, integrated card holder releases clamping simultaneously will be integrated Reagent card is positioned over correspondence station in linear slide, and linear synchronizing propeller returns back to a upper work together with integrated card holder Position, then proceedes to next wheel clamping, propulsion, homing action.

Further, linear synchronous propeller includes integrated card holder and propulsion motor, wherein integrated card holder Including integrated card clamping beam, bar linkage structure and clamping motor, integrated card clamping beam is a pair, and each integrated card clamping beam is arranged at On the outside of correspondence linear slide, integrated card clamping beam is provided with some clip tooths, and two integrated card clamping beams pass through bar linkage structure and clamping Motor, which is connected, realizes holding action, and integrated card holder is connected with propulsion motor realizes that reach is acted.

Further, sample filling apparatus includes sample injector support and sample injector, and sample injector branch is erected on support frame, both sides Line line slideway is located in sample injector support, and sample injector is arranged on the crossbeam of sample injector support and slid along the crossbeam, sample injector Including sample-adding control structure and the sample introduction head being connected, sample-adding control structure controls sample introduction head to the integrated reagent card of sample-adding station The quantitative sample of injection in upper each reagent cup, the crossbeam of sample injector support is provided with some optical sensors control sample injector displacement whereabouts Realization is in place to be detected and is loaded product, is not leaked and is added, does not also add by mistake.

Further, reaction solution filling apparatus includes reaction solution sample introduction support and reaction solution injector, reaction solution sample introduction support It is erected on support frame, both sides linear slide is located in reaction solution sample introduction support, and reaction solution injector is arranged on reaction solution sample introduction Slid on the crossbeam of support and along the crossbeam, reaction solution injector includes reaction solution sample introduction control structure and the sample introduction being connected Head, reaction solution sample introduction control structure control sample introduction head injects quantitative into each reagent cup on the integrated reagent card for add reaction solution station Reaction solution, the crossbeam of reaction solution sample introduction support is realized in place provided with some optical sensors control reaction solution injector displacement whereabouts Detect and be loaded product, do not leak and add, also do not add by mistake.

Further, negative reaction device includes negative reaction device support, top board, negative pressure control making sheet and negative pressure cavity, vavuum pump, Top board and negative pressure control making sheet are connected in reactor frame and can move towards and clamp on the integrated reagent card of reaction station Each integrated Reagent Tube, negative pressure control making sheet is provided with the film big envelope coordinated with each reagent cup, and film big envelope is top-opening type hatch frame, respectively Film big envelope is connected by negative-pressure guide pipe with negative pressure cavity and vavuum pump.

Further, the top board of negative reaction device pushes down the edge on the cup of integrated Reagent Tube, and negative pressure control making sheet is put down upwards Row movement, opens film big envelope on the mouth of pipe top of integrated Reagent Tube and inserts, make integrated Reagent Tube communicate to form negative pressure shape with negative pressure cavity State, the negative pressure of vavuum pump formation drives sample and reaction solution in integrated Reagent Tube to move downward to be formed and wear film reaction, vavuum pump shape Into flow-controllable and the negative pressure power of time.

Further, negative pressure cavity is connected by negative-pressure guide pipe with waste liquid bottle, and airway tube and surge flask are passed through at the top of waste liquid bottle It is connected, surge flask is connected by airway tube with vavuum pump.

Further, negative pressure wash mill includes washing liquid device support, washing lotion sample injector, film big envelope, negative pressure cavity and vavuum pump, washes Liquid device branch is erected on support frame, and washing lotion sample injector is several and is fixed on the crossbeam of washing liquid device support, and film big envelope is located at Below washing lotion sample injector, film big envelope is corresponding with the cooperation of integrated Reagent Tube, and film big envelope is top-opening type hatch frame, and each film big envelope passes through Negative-pressure guide pipe is connected with negative pressure cavity and vavuum pump, and sample introduction head and the film big envelope of washing lotion sample injector are moved towards, washing lotion sample injector Sample introduction head downwardly against washing station integrated reagent cup edge suitable for reading, and by pressure sensor open outlet discharge wash Liquid, the drain tube top of reagent cup opens film big envelope, makes integrated reagent cup communicate to form negative pressure state with negative pressure cavity, by integrated reagent cup Intracavitary residue, scrub raffinate are evacuated in the waste liquid bottle outside pipe.

Further, luminous substrate liquid adding set includes luminescent support, luminescent solution sample feeding controller and sample introduction needle, and light branch Frame is arranged on support frame, and luminescent solution sample feeding controller is arranged on the crossbeam of luminescent support and can slid along the crossbeam, luminescent solution Sample feeding controller connection sample introduction needle injects quantitative luminescence substrate on the integrated reagent card for the station that lights in each integrated reagent cup Liquid.

Further, integrated reagent card has several and integrated examination to be molded in the middle of one-time formed plastics card structure, the card There is the wedge structure concaved at the hole of agent matching, integrated reagent card two ends for the clip tooth clamping of integrated card clamping beam and promotes collection Into card movement, the reagent cup of integrated reagent is made up of cup and catheter, cup intracavitary have fixed dry type label film and Solid phase has antigen/antibody on solid phase membrane structure, immobilon-p, and immobilization antigen/antibody and sample on area, membrane fiber are formed for reaction In object to be measured combine, and with label formation immune response chain on label film, unreacted reactant worn downwards by intermembrane space Film, which is divided a word with a hyphen at the end of a line, to be drained into outside reagent cup.Integrated reagent is to be loaded into by integrator intelligent integrated on integrated card, constitutes one and patient is treated Survey the project implementation whole（The overwhelming majority is not in this way, complete another integrated reagent card integrated again once）Project team's parallel detection Integrated reagent card.

The utility model is after adopting the above technical scheme, integrated reagent set walks propulsion, one group of group according to the time interval of setting Integrated reagent card to be Reagent Project group integrated and gradually push luminous substrate addition position to by any project team, complete all detections Processing procedure, until luminous value determines and reported result, makes patient's detection project be promoted and completed parallel anti-in groups of mode Should, to realize that efficient electrochemiluminescent immunoassay instant in groups is detected and analyzed.

The beneficial effects of the utility model are as follows：

1. make the multiple determination project of each patient with burst mode parallel processing and reaction, improve external electrochemiluminescent immunoassay The instant detection efficiency of multi objective.

2. greatly shorten the reacting space time between each patient between detection project group, make the stand-by period of patient Greatly shorten, full-page proof processing during in favor of compared with multisample.

3. the utility model can realize the composite reaction of any project team of multi objective, greatly increase instrument and be applicable detection Mesh scope, it is ensured that multi objective, the detection of many diseases, diagnosis need.

Brief description of the drawings

Fig. 1 is the utility model embodiment schematic diagram.

Fig. 2 is the integrated reagent set parallel processing reactor schematic diagram of the utility model.

Fig. 3 is the integrated reagent set of the utility model washing and addition luminous substrate liquid device schematic diagram parallel.

Fig. 4 is the integrated reagent card of the utility model and integrated reagent schematic diagram.

Embodiment

The utility model is described in further detail below in conjunction with the accompanying drawings：

Embodiment：Fig. 1 is referred to Fig. 4, a kind of integrated linear propulsion parallel processing reaction unit of reagent set, it includes branch Support 11, the both sides of support frame 11 are provided with the linear slide 12 being engaged, and linear slide 12 is provided with some stations along slideway direction, such as A, B, C, D, E, F, G, H, I, J, K, M, N in Fig. 1, linear slide 12 are combined with the integrated card clamping dress of linear synchronous propeller 2 Put for clamping integrated reagent card 3, linear synchronous propeller 2 promotes integrated reagent card 3 to move forward and reset along linear slide 12, edge Sample filling apparatus 4, reaction solution filling apparatus 5, negative reaction device 6, negative pressure wash mill 7 and hair are sequentially provided with linear slide Light substrate solution adding set 8, the integrated reagent of the integrated preloaded of reagent card 3 completes sample-adding, reaction, washing and luminescent solution addition and made Industry.

As shown in figure 1, at least one group integrated concurrent job of reagent card 3 in linear slide 12, can be 2 groups, 3 groups or many The integrated reagent card concurrent job of group, each integrated reagent card group is controlled synchronously to move forward along linear slide by linear synchronous propeller 2, is collected Integrated reagent card 3 is clamped into card holder, linear synchronizing propeller 2 promotes integrated card holder and integrated reagent card whole Body one station of advance, integrated card holder releases clamping and integrated reagent card simultaneously is positioned over into correspondence station in linear slide, Linear synchronizing propeller returns back to a upper station together with integrated card holder, then proceedes to next wheel clamping, promotes, resets Action.The utility model linear synchronous propeller 2 includes integrated card holder and propulsion motor 21, wherein integrated card clamping dress Put including integrated card clamping beam 22, bar linkage structure and clamping motor 23, bar linkage structure includes longeron 24, cross bar 25, vertical rod 26 And motor link 27, integrated card clamping beam 22 is a pair, and each integrated card clamping beam is arranged at the outside of correspondence linear slide 12, collection Some clip tooths 28 are provided with into card clamping beam, two integrated card clamping beams are connected by bar linkage structure with clamping motor 23 realizes clamping Action, the longeron of integrated card holder is connected by propulsion motor connecting rod 29 with propulsion motor realizes that reach is acted.

As shown in figure 1, the utility model sample filling apparatus 4 includes sample injector support 40 and sample injector, sample injector support 40 are located on support frame 11, and two side line line slideways 12 are located in sample injector support, and sample injector 41 is arranged on the horizontal stroke of sample injector support Slid on beam 41 and along the crossbeam, sample injector includes sample-adding control structure 42 and the sample introduction head 43 being connected, be loaded control structure Sample introduction head is controlled to be set into each reagent cup on the integrated reagent card of sample-adding station on the quantitative sample of injection, the crossbeam of sample injector support There are some optical sensor control sample injectors to shift whereabouts and realize detection in place and sample-adding product, do not leak and add, also do not add by mistake.

As shown in Figures 1 and 2, reaction solution filling apparatus 5 includes reaction solution sample introduction support 50 and reaction solution injector, reaction Liquid sample introduction support 50 is erected on support frame 11, and both sides linear slide 12 is located in reaction solution sample introduction support, reaction solution injector It is arranged on the crossbeam 51 of reaction solution sample introduction support and is slid along the crossbeam, reaction solution injector includes reaction solution sample introduction and controls knot Structure 52 and the sample introduction head 53 being connected, reaction solution sample introduction control structure 52 control sample introduction head 53 to the integrated examination for adding reaction solution station Injection quantitative reaction liquid in each reagent cup on agent card, the crossbeam 51 of reaction solution sample introduction support 50 is controlled provided with some optical sensors The displacement whereabouts realization of reaction solution injector is in place to be detected and sample-adding product, is not leaked and is added, does not also add by mistake.Negative reaction device 6 includes negative pressure Reactor frame 61, top board 62, negative pressure control making sheet 63 and negative pressure cavity 64, vavuum pump 65, top board 62 and negative pressure control making sheet 63 It is connected in reactor frame 61 and can moves towards and clamp each integrated Reagent Tube or cup on the integrated reagent card of reaction station, Negative pressure control making sheet 63 is provided with the film big envelope 68 coordinated with each reagent cup, and film big envelope 68 is top-opening type hatch frame, film big envelope 68 Opening at form multiple film mountings 681, such as cross open or rice word opening, each film big envelope by negative-pressure guide pipe 640 with it is negative Pressure chamber 64 and vavuum pump 65 are connected.The upper edge-pressing strip 621 of negative reaction device top board pushes down the edge on the cup of integrated Reagent Tube, Negative pressure control making sheet 63 is moved in parallel upwards, is opened film big envelope in the mouth of pipe or drain tube top of integrated Reagent Tube cup and is inserted, makes integrated Reagent Tube communicates to form negative pressure state with negative pressure cavity, the negative pressure of vavuum pump formation drive in integrated Reagent Tube sample and reaction solution to Film reaction, vavuum pump formation flow-controllable and the negative pressure power of time are worn in lower motion formation.Negative pressure cavity 64 passes through negative-pressure guide pipe 640 connect with waste liquid bottle 66, are connected at the top of waste liquid bottle by airway tube with surge flask, surge flask passes through airway tube and vavuum pump 65 Connection.

As shown in Figures 1 and 3, negative pressure wash mill 7 include washing liquid device support 70, it is washing lotion sample injector 71, film big envelope 72, negative Chamber 73 and vavuum pump 74 are pressed, washing liquid device support 70 is located on support frame 11, washing lotion sample injector 71 is several and is fixed on washing lotion On the crossbeam of device support, film big envelope 72 is located at the lower section of washing lotion sample injector 71, and film big envelope is corresponding with the cooperation of integrated Reagent Tube, film big envelope 72 be to form multiple film mountings, such as cross open or rice word opening, each film big envelope at top-opening type hatch frame, the opening of film big envelope It is connected by negative-pressure guide pipe 730 with negative pressure cavity 73 and vavuum pump 74, the sample introduction head and film big envelope 72 of washing lotion sample injector 71 are opposite Mobile, the sample introduction head of washing lotion sample injector 71 and passes through pressure sensing downwardly against the integrated reagent cup edge suitable for reading of washing station Device 701 opens outlet discharge washing lotion, and the drain tube top of reagent cup opens film big envelope, makes integrated reagent cup communicate to form negative with negative pressure cavity Pressure condition, integrated reagent cup intracavitary residue, scrub raffinate are evacuated in the waste liquid bottle 75 outside pipe, waste liquid bottle 75 and vavuum pump it Between be additionally provided with surge flask 76.Luminous substrate liquid adding set 8 includes luminescent support 80, luminescent solution sample feeding controller 81 and sample introduction needle 82, luminescent support 80 is arranged on support frame 11, and luminescent solution sample feeding controller is arranged on the crossbeam of luminescent support 80 and energy edge should Crossbeam slides, the connection sample introduction needle 82 of luminescent solution sample feeding controller 81 each integrated reagent cup on the integrated reagent card for the station that lights Interior injection quantitative luminescence substrate solution.

Such as Fig. 1 and Fig. 4, integrated reagent card 3 has several and collection to be molded in the middle of one-time formed plastics card structure, the card The hole 31 matched into reagent 32, the integrated two ends of reagent card 3 have the wedge structure concaved or groove 33 to supply integrated card clamping beam Clip tooth clamping and promote integrated card movement, the reagent cup of integrated reagent is made up of cup and catheter, and cup intracavitary has solid Solid phase has antigen/antibody on fixed dry type label film and solid phase membrane structure, immobilon-p, forms solid on area, membrane fiber for reaction Phaseization antigen/antibody is combined with object to be measured in sample, and forms immune response chain, unreacted with label on label film Thing, which is worn downwards film by intermembrane space and divided a word with a hyphen at the end of a line, to be drained into outside reagent cup.Integrated reagent is to be loaded into integrated card by integrator intelligent integrated On, one is constituted to patient's project implementation to be measured whole（The overwhelming majority is not in this way, complete another integrated reagent integrated again once Card）The integrated reagent card of project team's parallel detection.

As shown in Figure 1 to Figure 4, the linear propulsion parallel processing reaction unit of a kind of integrated reagent set, i.e., refer to a kind of immune detection more The linear propulsion parallel processing of integrated reagent set and reaction unit are marked, linear synchronous propeller is described as follows, linear synchronizing propeller Propulsion motor and the linear synchronizing propeller and linear track composition of the clamping motor driving of integrated reagent card, linear track are located at line On the inside of shape synchronizing propeller, be provided with track for integrated reagent card carry out the station A of corresponding operating, B, C, D, E, F, G, H, I, J, K, M, N, coordinate with other structures, are respectively completed respective handling process herein.Linear synchronizing propeller is located under linear track Portion and outside.Linear synchronizing propeller propeller includes clamping, propulsion motor and the connecting rod being attached thereto and integrated card clamping knot Structure；Linear synchronizing propeller promotes multigroup integrated reagent card synchronously to divide a word with a hyphen at the end of a line a station under propulsion motor driving, then resets. Integrated card holding structure is pushed away including clamping motor, integrated card clamping motor, connecting rod, linear synchronizing propeller longeron, linear synchronization Enter device crossbeam, linear synchronizing propeller vertical rod, linear synchronizing propeller integrated card clamping beam, linear synchronizing propeller longeron collection Into gripper teeth.During work, clip tooth clamps integrated reagent card two ends groove under clamping motor driving, and online shape synchronizing propeller exists Together with linear synchronizing propeller propeller, integrated reagent card integrally one station of parallel advance, then will collection under propulsion motor driving Linear track corresponding A is positioned over into reagent card --- on N stations.Clamp motor and release clamping, linear synchronizing propeller is being promoted Returned back under motor driving together with linear synchronizing propeller propeller, integrated reagent card it is upper, it is static as defined in after the time, continue Clamping, propulsion, the action resetted of next setting.In this way, each integrated reagent card is completed from A stations to the whole of N stations Operation, completes reaction and corresponding processing.Sample filling apparatus is fixed on linear synchronizing propeller integrated card clamping beam including one Sample injector support outside on the side, the sample injector slideway along the longitudinal traveling of integrated reagent card（That is crossbeam）, one can be fixed along slideway Sample injector control structure and sample injector sample introduction head that point is slided.When the integrated reagent card of Reagent Project group enters sample-adding station B, instrument Device starts sampling action, and sample injector control structure is completed after sampling by the sample injector sample introduction head alignment integrated Reagent Tube to be loaded （Integrated reagent cup）, the sample of ormal weight is injected into integrated Reagent Tube.And rule are injected into each integrated reagent successively along slideway Quantitative sample, until reagent is fully integrated in same integrated card completes sample-adding, integrated each reagent of reagent card enters reactiveness. The integrated reagent card for having added sample is advanced into next station C again and reacted by linear synchronizing propeller.Described slideway（Sample-adding Device rack beam）Sample pipetting volume device control structure whereabouts are controlled provided with optical sensor, detection in place and sample-adding product is realized, does not leak Plus, also do not add by mistake.Reaction solution filling apparatus includes one and is fixed on outside linear synchronizing propeller integrated card clamping beam on the side anti- Answer liquid sample introduction support, horizontal reaction solution sample introduction slideway along the longitudinal traveling of integrated reagent card, one slide anti-can be pinpointed along slideway Answer liquid sample introduction control structure and reaction solution sample introduction head.When the integrated reagent card of Reagent Project group, which enters, adds reaction solution station E, instrument Start filling reaction solution action, reaction solution sample introduction control structure complete sampling after by reaction solution sample introduction head be aligned to be loaded it is integrated Reagent Tube（Integrated reagent cup）, the reaction solution of ormal weight is injected into integrated Reagent Tube, and along slideway successively to each integrated reagent It is interior injection ormal weight reaction solution, until be fully integrated reagent complete filling reaction solution, with wash integrated Reagent Tube sample cavity and Label film, integrated each reagent of reagent card continues to react.Linear synchronizing propeller will add the integrated reagent card of reaction solution again It is advanced into next station F reactions.Described slideway controls reaction solution sample introduction control structure whereabouts provided with optical sensor, realizes It is in place to detect and add reaction solution, do not leak and add, also do not add by mistake.Negative reaction device is driven by negative reaction device support, negative reaction device Motor, top board support, top board, edge-pressing strip, negative pressure board mount, negative pressure control making sheet, top-opening type film big envelope, top-opening type film envelope It is piece, negative-pressure guide pipe, negative pressure cavity, negative-pressure guide pipe, waste liquid bottle, waste liquid row mouth control valve, airway tube, surge flask, negative pressure cavity, true Empty pump group into.The integrated online shape synchronizing propeller of reagent card enters in station F, negative reaction device drive motor under promoting presses Plate and negative pressure control making sheet relative motion.Top board pushes down the edge on integrated reagent cup cup is reacted, and bottom negative pressure control making sheet is upward Move in parallel, open film mounting reacting integrated reagent drain tube top, integrated reagent reacting pipe bottom is entered negative pressure state, drive Sample and reaction solution are moved downward in integrated Reagent Tube, and film reaction is worn in formation.Described negative pressure device under vacuum pumping action, in Negative pressure is formed in each pipeline and bottle, and in flow-controllable and negative pressure time state.Each integrated reagent card undergoes several Negative pressure wears film reaction process, until whole samples, which are realized, wears film reaction, ensures the accuracy of testing result to greatest extent.Negative pressure is washed Wash device and control structure, washing lotion sample introduction head, pressure sensor, top are filled by washing liquid device support, washing lotion sample introduction head frame, washing lotion Open type film big envelope is arranged mouth control valve, airway tube with film mounting drive motor, negative-pressure guide pipe, negative pressure cavity, waste liquid bottle, waste liquid, delayed Rush bottle, negative pressure cavity, vacuum pump group into.When integrated reagent card enters M stations, washing lotion sample introduction head, top-opening type film big envelope and film mounting Move toward one another.Washing lotion sample introduction head pushes down integrated Reagent Tube edge suitable for reading, and opens washing lotion head discharge washing lotion by pressure sensor. Integrated reagent water conservancy diversion tube top opens film mounting, integrated reagent reacting pipe bottom is entered negative pressure state, and integrated Reagent Tube intracavitary is residual Thing, scrub raffinate is stayed to be evacuated to outside pipe.So rush, take out, arrange, fully washing, until washing to the defined time.Luminous substrate liquid adds Feeder apparatus by luminous substrate liquid add structure stand, luminous substrate liquid sample feeding controller, luminous substrate liquid sample introduction needle constitute. When integrated reagent card enters N stations, instrument starts luminous substrate liquid sample feeding controller, and luminous substrate liquid sample introduction needle is along luminous substrate Liquid sample introduction slideway adds the luminous substrate liquid of ormal weight into each integrated reagent reacting pipe successively, until it is luminous to complete all additions Substrate solution.Luminescence assays darkroom is transferred to by other mechanisms again added with the integrated reagent reacting pipe of luminous substrate liquid, is determined one by one Individual integrated Reagent Tube relative luminous intensity, calculates object content, and each integrated reagent correspondence target concentration is reported parallel and is sentenced Determine result.

As shown in Figure 1 to Figure 4, the linear propulsion parallel processing reaction unit of a kind of integrated reagent set, i.e., refer to a kind of immune detection more Mark the linear propulsion parallel processing of integrated reagent set and reaction unit, a kind of linear propulsion parallel processing of the integrated reagent set of immune detection With reaction unit, including linear synchronizing propeller and track, sample filling apparatus, reaction solution filling apparatus and negative reaction device, The composition such as negative pressure wash mill, luminous substrate addition structure, integrated reagent card and integrated reagent, realize from sample-adding product to washing, Plus luminous substrate walks operation processing parallel, while reporting result.

As shown in Fig. 1 and Fig. 4, integrated card is that the integrated load reagents position of integrated card or hole 31 of integrated reagent card 3 are loaded Enter fixed support after integrated reagent 32（Support frame 11）On linear slideway 12 A stations.Integrated card clamping motor 21 passes through Integrated card clamping motor link 29, linear synchronizing propeller longeron 24, linear synchronizing propeller cross bar 25, linear synchronizing propeller Vertical rod 26, linear synchronizing propeller integrated card clamping beam 22, drive the linear integrated gripper teeth 28 of synchronizing propeller longeron to clamp The groove of integrated reagent card 3 of integrated reagent is mounted with, along the online shape synchronizing propeller propulsion motor 21 of linear track and linear same Walk under the effect of propeller propulsion motor connection-rod linkage, travelling to sample by A stations adds position B stations, implements sample introduction.

As shown in figure 1, the sample injector control structure 42 on sample injector support 40 is advanced into along sample injector slideway 41 and waits to be loaded The integrated reagent position of integrated reagent card, the sample of set amount is added to integrated Reagent Tube by sample injector sample introduction head 43.Complete a collection Into after Reagent Tube sample introduction, the sample of ormal weight is injected into each integrated reagent successively along slideway, until in same integrated reagent card It is fully integrated reagent and completes sample-adding, integrated each reagent of reagent card enters reactiveness.Described slideway is provided with optical sensor control Sample preparation product sample injector control structure whereabouts, realization is in place to be detected and is loaded product, is not leaked and is added, does not also add by mistake.Linear synchronizing propeller The integrated reagent card for having added sample is advanced into the reaction of C, D, E station successively again.

As shown in figure 1, when integrated reagent card is in E stations, the reaction solution sample introduction control on reaction solution sample introduction support 50 Structure 52 is advanced into the integrated reagent position of the integrated reagent card of reaction solution to be added along reaction solution sample introduction slideway 51, by reaction solution sample introduction head 53 The reaction solution of set amount is added to integrated Reagent Tube.Complete after an integrated Reagent Tube addition reaction solution, along slideway successively to each The reaction solution of injection ormal weight in integrated reagent, until reagent is fully integrated in same integrated reagent card completes addition reaction solution, Integrated each reagent of reagent card continues reactiveness.Described slideway controls reaction solution sample injector control structure provided with optical sensor Whereabouts, realization is in place to be detected and adds reaction solution, is not leaked and is added, does not also add by mistake.Linear synchronizing propeller will add reaction solution again Integrated reagent card is advanced into the reaction of F stations.

As shown in figure 1, when integrated reagent card is in F stations, the negative reaction device driving on negative reaction device support 61 Motor 601 drives the edge-pressing strip 621 on top board 62 to push down on integrated reagent cup 603 is reacted via top board support 602 Opening's edge, while negative reaction device drive motor 601 drives the motion upwards of negative pressure control making sheet 63 via negative pressure board mount 604, anti- Integrated reagent catheter 6030 is answered to be contacted with top-opening type film big envelope 64, top is opened top-opening type film mounting 641 and connect by negative-pressure guide pipe 640 Enter negative pressure cavity 64, integrated reagent card has integrated reagent position to enter negative pressure leaching reactiveness.Work what is formed in vavuum pump 65 Under suction function, via negative pressure cavity 64, airway tube 660 and surge flask 67, waste gas, waste liquid are drained into by the water conservancy diversion of negative-pressure guide pipe 640 Waste liquid bottle 66, so completes negative pressure and wears film reaction.In linear track F-K, realize that negative pressure wears film reaction process.Linear synchronization The integrated reagent card for having completed negative pressure and wearing film reaction is advanced into the washing of M stations by propeller again.

As shown in figure 1, when integrated reagent card is in M stations, the washing lotion sample introduction head on washing liquid device support 70 is fixed Frame（Crossbeam）, the washing lotion sample introduction head of each washing lotion sample injector 71 is controlled in washing lotion filling control structure and pressure sensor 701 is acted on Under, it is descending to push down integrated reagent upper edge hole, liquid injecting function is opened, cleaning solution is filled to there is integrated reagent position.So simultaneously, top is opened Formula film big envelope 72 is moved upwards with film mounting drive motor, and integrated reagent catheter is contacted with top-opening type film big envelope 72, top Open top-opening type film mounting and negative pressure cavity 73 is accessed by negative-pressure guide pipe 730, integrated reagent card has integrated reagent position to enter negative pressure leaching Washing state.Work under formed vacuum action, via negative pressure cavity 73, airway tube 750 and surge flask 76, give up in vavuum pump 74 Gas, waste liquid drain into waste liquid bottle 75 by the water conservancy diversion of negative-pressure guide pipe 730, so complete negative pressure washing.Linear synchronizing propeller is again by The integrated reagent card that completion negative pressure wears film reaction is advanced into N stations addition luminous substrate liquid.

As shown in figure 1, when integrated reagent card is in N stations, the luminous bottom on luminous substrate liquid addition structure stand 80 Thing liquid sample feeding controller 81 is along luminous substrate liquid sample introduction slideway（Crossbeam）It is advanced into the integrated reagent card of luminous substrate liquid to be added integrated Reagent position, the luminous substrate liquid of set amount is added to integrated Reagent Tube by luminous substrate liquid sample introduction needle 82.Complete an integrated examination After agent pipe addition luminous substrate liquid, the luminous substrate liquid of ormal weight is injected into each integrated reagent successively along slideway, until same Reagent is fully integrated in integrated reagent card and completes addition luminous substrate liquid, integrated each reagent of reagent card enters luminescence-producing reaction state. Luminescence assays darkroom is transferred to by other mechanisms again added with the integrated reagent reacting pipe of luminous substrate liquid, an integrated examination is determined one by one Agent pipe relative luminous intensity, calculates object content, and each integrated reagent correspondence target concentration and result of determination are reported parallel, complete Into the detection operation of an integrated reagent card.

Described above, only using the embodiment of this origination techniques content, any those skilled in the art use this wound Make done modifications and changes, all belong to the scope of the claims of this creation opinion, and be not limited to those disclosed embodiments.

Claims (10)

1. a kind of linear propulsion parallel processing reaction unit of integrated reagent set, it is characterised in that：Including support frame, support frame both sides Provided with the linear slide being engaged, linear slide is provided with some stations along slideway direction, and linear slide is combined with linear synchronous and pushed away Entering the integrated card holder of device is used to clamp integrated reagent card, and linear synchronous propeller promotes integrated reagent card along linear slide Move forward and reset, sample filling apparatus, reaction solution filling apparatus, negative reaction device, negative pressure are sequentially provided with along in linear slide and is washed Device and luminous substrate liquid adding set are washed, the integrated reagent of integrated reagent card preloaded completes sample-adding, reaction, washing and lighted Liquid adds operation.

2. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to claim 1, it is characterised in that：Line Property slideway at least one group integrated reagent card concurrent job, each integrated reagent card group is by the synchronous edge of linear synchronous propeller control Linear slide moves forward, the integrated reagent card of integrated card gripping means grips, and linear synchronizing propeller promotes integrated card holder And integrated reagent card integrally advances a station, integrated card holder releases to clamp simultaneously is positioned over linear cunning by integrated reagent card Correspondence station on road, linear synchronizing propeller returns back to a upper station together with integrated card holder, then proceedes to next wheel Clamping, propulsion, homing action.

3. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to claim 1, it is characterised in that：Line Property synchronizing propeller include integrated card holder and propulsion motor, wherein integrated card holder include integrated card clamping beam, Bar linkage structure and clamping motor, integrated card clamping beam is a pair, and each integrated card clamping beam is arranged on the outside of correspondence linear slide, Integrated card clamping beam is provided with some clip tooths, and two integrated card clamping beams are connected by bar linkage structure with clamping motor realizes that clamping is dynamic Make, being connected with propulsion motor for integrated card holder realizes that reach is acted.

4. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to claim 1, it is characterised in that：Sample Product filling apparatus includes sample injector support and sample injector, and sample injector branch is erected on support frame, and two side line line slideways are located at sample-adding In device support, sample injector is arranged on the crossbeam of sample injector support and slid along the crossbeam, and sample injector includes sample-adding control structure With the sample introduction head being connected, sample-adding control structure controls sample introduction head to be injected into each reagent cup on the integrated reagent card of sample-adding station Quantitative sample, the crossbeam of sample injector support shifts whereabouts provided with some optical sensors control sample injector and realizes detection in place with adding Sample.

5. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to claim 1, it is characterised in that：Instead Liquid filling apparatus is answered to include reaction solution sample introduction support and reaction solution injector, reaction solution sample introduction support is erected on support frame, two Linear slideway be located at reaction solution sample introduction support in, reaction solution injector be arranged on the crossbeam of reaction solution sample introduction support and along this Crossbeam slides, and reaction solution injector includes reaction solution sample introduction control structure and the sample introduction head being connected, reaction solution sample introduction control knot Structure control sample introduction head injects quantitative reaction liquid, reaction solution sample introduction branch into each reagent cup on the integrated reagent card for add reaction solution station The crossbeam of frame shifts whereabouts provided with some optical sensors control reaction solution injector and realizes detection in place with adding reaction solution.

6. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to claim 1, it is characterised in that：It is negative Reactor is pressed to include negative reaction device support, top board, negative pressure control making sheet and negative pressure cavity, vavuum pump, top board and vacuum cavitations Plate is connected in reactor frame and can move towards and clamp each integrated Reagent Tube, negative pressure on the integrated reagent card of reaction station Control panel is provided with the film big envelope coordinated with each reagent cup, and film big envelope is top-opening type hatch frame, and each film big envelope is led by negative pressure Flow tube is connected with negative pressure cavity and vavuum pump.

7. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to claim 1, it is characterised in that：It is negative The top board of pressure reactor pushes down the edge on the cup of integrated Reagent Tube, and negative pressure control making sheet is moved in parallel upwards, in integrated reagent The mouth of pipe top of pipe is opened film big envelope and inserted, and makes integrated Reagent Tube communicate to form negative pressure state with negative pressure cavity, and it is negative that vavuum pump is formed Sample and reaction solution move downward to be formed and wear film reaction in the integrated Reagent Tube of pressure driving, vavuum pump formation flow-controllable and time Negative pressure power, negative pressure cavity is connected by negative-pressure guide pipe with waste liquid bottle, is connected at the top of waste liquid bottle by airway tube with surge flask, is delayed Bottle is rushed to be connected with vavuum pump by airway tube.

8. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to claim 1, it is characterised in that：It is negative Wash mill is pressed to include washing liquid device support, washing lotion sample injector, film big envelope, negative pressure cavity and vavuum pump, washing liquid device branch is erected at support On frame, washing lotion sample injector is several and is fixed on the crossbeam of washing liquid device support, and film big envelope is located at below washing lotion sample injector, film Big envelope and integrated Reagent Tube coordinate, and film big envelope is top-opening type hatch frame, each film big envelope by negative-pressure guide pipe and negative pressure cavity and Vavuum pump is connected, and sample introduction head and the film big envelope of washing lotion sample injector are moved towards, and the sample introduction head of washing lotion sample injector is downwardly against washing The integrated reagent cup edge suitable for reading of station, and discharge washing lotion in outlet is opened by pressure sensor, the drain tube top of reagent cup is opened Film big envelope, makes integrated reagent cup communicate to form negative pressure state with negative pressure cavity, and integrated reagent cup intracavitary residue, scrub raffinate are taken out In waste liquid bottle outside to pipe.

9. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to claim 1, it is characterised in that：Hair Light substrate solution adding set includes luminescent support, luminescent solution sample feeding controller and sample introduction needle, and luminescent support is arranged on support frame, Luminescent solution sample feeding controller is arranged on the crossbeam of luminescent support and can slid along the crossbeam, luminescent solution sample feeding controller connection sample introduction Pin injects quantitative luminescence substrate solution on the integrated reagent card for the station that lights in each integrated reagent cup.

10. a kind of integrated linear propulsion parallel processing reaction unit of reagent set according to one of claim 1 to 9, it is special Levy and be：Integrated reagent card has what several were matched with integrated reagent to be molded in the middle of one-time formed plastics card structure, the card There is the wedge structure concaved at hole, integrated reagent card two ends for the clip tooth clamping of integrated card clamping beam and promotes integrated card to move Dynamic, the reagent cup of integrated reagent is made up of cup and catheter, and cup intracavitary has fixed dry type label film and immobilon-p Solid phase has antigen/antibody on structure, immobilon-p, for reaction formed on area, membrane fiber immobilization antigen/antibody with it is to be measured in sample Object is combined, and forms immune response chain with label on label film, and unreacted reactant is worn downwards film by intermembrane space and divided a word with a hyphen at the end of a line Drain into outside reagent cup.