CN110220890A - Vaginal fluid detector - Google Patents
Vaginal fluid detector Download PDFInfo
- Publication number
- CN110220890A CN110220890A CN201910429753.7A CN201910429753A CN110220890A CN 110220890 A CN110220890 A CN 110220890A CN 201910429753 A CN201910429753 A CN 201910429753A CN 110220890 A CN110220890 A CN 110220890A
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- sample
- reagent
- test paper
- conveyer belt
- paper strip
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- 239000012530 fluid Substances 0.000 title claims abstract description 25
- 238000012360 testing method Methods 0.000 claims abstract description 110
- 238000001514 detection method Methods 0.000 claims abstract description 47
- 238000007689 inspection Methods 0.000 claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims description 116
- 239000007788 liquid Substances 0.000 claims description 46
- 238000004140 cleaning Methods 0.000 claims description 35
- 108010055851 Acetylglucosaminidase Proteins 0.000 claims description 17
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 16
- 102000005348 Neuraminidase Human genes 0.000 claims description 15
- 108010006232 Neuraminidase Proteins 0.000 claims description 15
- 239000007921 spray Substances 0.000 claims description 15
- 239000002699 waste material Substances 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 108010000849 leukocyte esterase Proteins 0.000 claims description 8
- 230000002572 peristaltic effect Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 230000005611 electricity Effects 0.000 claims description 7
- 230000002255 enzymatic effect Effects 0.000 claims description 6
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 5
- 230000001360 synchronised effect Effects 0.000 claims description 4
- 229930182470 glycoside Natural products 0.000 claims description 3
- 230000032258 transport Effects 0.000 claims description 3
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 claims description 2
- 230000007423 decrease Effects 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 150000002338 glycosides Chemical class 0.000 claims 2
- 102000004400 Aminopeptidases Human genes 0.000 claims 1
- 108090000915 Aminopeptidases Proteins 0.000 claims 1
- DUKURNFHYQXCJG-UHFFFAOYSA-N Lewis A pentasaccharide Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(C(O)C(O)C(CO)O2)O)C(NC(C)=O)C(OC2C(C(OC3C(OC(O)C(O)C3O)CO)OC(CO)C2O)O)OC1CO DUKURNFHYQXCJG-UHFFFAOYSA-N 0.000 claims 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 claims 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
- 229950006780 n-acetylglucosamine Drugs 0.000 claims 1
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000005540 biological transmission Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 10
- 238000003794 Gram staining Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000004043 dyeing Methods 0.000 description 3
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 2
- 206010046914 Vaginal infection Diseases 0.000 description 2
- 201000008100 Vaginitis Diseases 0.000 description 2
- 210000003756 cervix mucus Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- IYAVKEQFCKMRHX-WCCKRBBISA-N azane;(2s)-pyrrolidine-2-carboxylic acid Chemical compound N.OC(=O)[C@@H]1CCCN1 IYAVKEQFCKMRHX-WCCKRBBISA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 235000015177 dried meat Nutrition 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000004996 female reproductive system Anatomy 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000003771 laboratory diagnosis Methods 0.000 description 1
- 201000005630 leukorrhea Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000011022 operating instruction Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012372 quality testing Methods 0.000 description 1
- -1 sialic acid glycosides Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 206010046901 vaginal discharge Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
Abstract
Vaginal fluid detector of the invention, it is characterised in that: including being mounted on sample adding device on body, transmission device, warm bath system, detection device, strip storage device and test paper strip.It is operated the invention has the advantages that being loaded with mechanical arm instead of traditional-handwork, greatly reduces the biohazard risk of reviewer, detect program standardization, accuracy is high, high degree of automation, the inspection suitable for high-throughput sample.
Description
Technical field
The present invention relates to technical field of medical detection, specially a kind of vaginal fluid detector.
Background technique
Vaginal fluid is the liquid of female reproductive system secretion, is commonly called as " leukorrhea ", intravaginal mixes vagina bacterial parasite
Group, it is general not pathogenic, it is in microecological balance state, if this balance is destroyed, generates corresponding inflammation or clinical condition
Hou Qun.It is inner by vaginal secretion quality testing in " national clinical examination operating instruction " (fourth edition) chapter 15 " vaginal fluid inspection "
It tests index and is divided into Neo-Confucianism inspection, chemical analysis, visible component analysis three classes index.
The detection of vaginal fluid visible component depends on microscopical observation, according to main to the processing mode of sample
It is divided into the direct Microscopical Method For Detection of humidity strip and dyeing microscopic examination method.Although humidity strip Microscopical Method For Detection is easy quickly, positive rate is low, omission factor
It is high.So developing to dyeing microscopic examination method, predominantly Gram staining, present Gram staining master in turn to improve its disadvantage
It to be manual Gram staining.The advantages that manual Gram staining has positive rate high, and omission factor is low, quickly fast.But
This method has in large sample size expends a large amount of manpowers, and pollutes environment, needs sink, occupies the disadvantages of work area is big,
Moreover, to vaginal fluid carry out visible component observation only have microscope be it is far from being enough, it cannot complete independent detection
The generation of report, the digitlization that also can not be completed detection data are saved, are inquired, browsing, and the functions such as printing, display are faced at present
Testing index is hospital's designed, designed on bed, and each hospital of project indicator syllabus and content does not unify, and standardization level is low, respectively
Testing result is difficult to recognize each other between hospital.Therefore, truly have needs to entire vaginal fluid decoration method detection process on clinical objective
Whole process optimizes, and to retain dyeing microscopic examination method advantage, abandons disadvantages mentioned above, can substantially save manpower in this way, carries out automatic
Change, improve original detection working efficiency, shorten detection time, improves standardized testing degree.And then finally improve vaginal secretion
Analyte detection accuracy rate reaches accurate and improves the horizontal purpose of vaginitis laboratory diagnosis.
Summary of the invention
A kind of in order to compensate for the shortcomings of the prior art, the present invention provides structures vaginal fluid simple, easy to use
Detector.
The present invention is achieved through the following technical solutions:
Vaginal fluid detector of the invention, it is characterised in that: including be mounted on body control device, sample adding device,
Conveying device；
Sample adding device includes the sample and reagent disc, specimen needle being mounted on body；Sample and reagent disc are provided with a large amount of sample
One's own department or unit and several reagent positions, each sample bit place a sample tube, SNA developing solution are stored in reagent position, for making test paper
Strip " neuraminidase hole " colour developing；Specimen needle connects body through manipulator, and specimen needle is deposited on sample and reagent disc for drawing
It is added drop-wise on the test paper strip on conveyer belt after the liquid sample or developing solution put；
Test paper strip is placed in conveying device and transports test paper strip runs by detecting step；Conveying device includes being mutually butted
Two traveling beds and two transverse onveyers, conveyer belt use servo motor drive control, each conveyer belt synchronous operation.
In conveying device, test paper strip storehouse is arranged in the front end of longitudinal first conveyer belt, places test paper in first conveyer belt
It is moved forwards after strip, so as to the sample or developing solution for making test paper strip receive to draw from specimen needle；Lateral second is defeated
Band front end and first conveyer belt end on is sent, receives the test paper strip from first conveyer belt end, at the second conveyer belt
Warm bath apparatus is set, and test paper strip carries out warm bath heating reaction；Longitudinal third conveyer belt front end and the second conveying end of tape pair
It connects, setting addition test paper strip " acetyl glucosaminidase hole " NAG terminate liquid sample adding device, makes above third conveyer belt
" acetyl glucosaminidase hole " terminates reaction；The front end of the 4th lateral conveyer belt and third conveyer belt end on, the
Setting test paper strip 5-linked examines detection device above four conveyer belts, reads color after test paper strip is reacted, output detection knot
Fruit, four end of the 4th conveyer belt are arranged useless Ka Cang, collect using the test paper strip finished.
Sample connect servo motor with reagent pan bottom and carries out driving rotation, and servo motor is mounted in body, to incite somebody to action
Sample is rotated to close to the side of conveying device；The manipulator that specimen needle is connected, including the sequentially connected electricity for lifting
The electric cylinders of cylinder, the servo motor of horizontal rotation, horizontal extension, wherein the electric cylinders for lifting connect body, the electricity of horizontal extension
Cylinder connects specimen needle, and specimen needle is using cylinder control sucking liquid and sprays liquid；Specimen needle declines after rotating to certain position
It is inserted into sample tube or reagent bottle, the SNA developing solution in the sample handled well or reagent bottle in sample tube is drawn, is then added drop-wise to
The test paper strip being placed in first conveyer belt.
It is also equipped with cleaning device on body, realizes that specimen needle draws the cleaning function after sample or reagent, cleaning device
Spray head use spring formula spray head or waterfall type spray head, cleaning device by the road with the cleaning solution in pump connection container, on body
It is also equipped with waste liquid pool, to catch the waste liquid after cleaning, waste liquid pool connects sewer through water pipe；Spray head can clean outside specimen needle
Side, specimen needle control sucking cleaning solution using cylinder and spray cleaning solution to clean inside.
Terminate liquid sample adding device includes hard sample-adding pipe and peristaltic pump interconnected, sample-adding pipe connection NAG terminate liquid examination
Agent container.
Warm bath apparatus uses constant-temperature air bath device, constant temperature water bath apparatus or constant temperature oil bath device, preferably uses constant temperature
Airshower；It is located at before warm bath apparatus at second conveyer belt and pH value detection device is set, test paper strip carries out pH value color
Detection is analyzed pH value and is exported.
It is provided with scanning bar code device on sample and reagent disc, scans the bar shaped in the sample tube being put into sample bit
Code.Sample bit on sample and reagent disc has 20-120, and reagent position has 2-12.
The vertical electric cylinders and lateral electric cylinders of cooperating, vertical electric cylinders installation are installed on test paper strip storehouse by connector
In orlop, lateral electric cylinders are mounted on silo roof, and gradually elongation is laterally electric to gradually push up test paper strip upwards for vertical electric cylinders
Cylinder one stretches a contracting to one by one shift uppermost test paper strip in first conveyer belt onto；Conveying device runs a step, test card
Storehouse just runs a step and pops up a test paper strip.
Test paper strip uses six joint inspection cards or five joint inspection cards, and six joint inspection cards are provided with hydrogen peroxide agent hole, leucocyte ester
Enzymatic reagent hole, neuraminidase reagent wells, Prolyl iminopeptidase reagent wells, acetyl glucosaminidase reagent wells, pH value
Six kinds of reagent wells of reagent wells；Five joint inspection cards do not have pH value reagent wells, remaining is identical as six joint inspection cards；The warm bath on the second conveyer belt
Heating purpose is to plant reagent, i.e. hydrogen peroxide agent hole, leukocyte esterase reagent wells, neuraminidase reagent wells, dried meat for first five
The reaction of sample provides suitable reaction temperature in histidine amino group peptase reagent wells, acetyl glucosaminidase reagent wells；Sample
This sample for being used for neuraminidase reagent wells with the SNA developing solution in reagent disc develops the color；NAG terminate liquid is for terminating acetyl ammonia
The reaction of sample in base glucuroide reagent wells；First five kind examination is read at the 4th conveyer belt at test paper strip 5-linked test examination instrument
Color after the reaction of agent, analysis detection result simultaneously export.
Control device uses computer, single-chip microcontroller or PLC controller, and control device controls each servo motor, cylinder, electricity
Cylinder, scanning bar code device, peristaltic pump, warm bath apparatus, pH value detection device, test paper strip 5-linked test examination instrument.These components
It is all existing product.
It is operated the invention has the advantages that being loaded with mechanical arm instead of traditional-handwork, greatly reduces identifier
The biohazard risk of member detects program standardization, and accuracy is high, high degree of automation, the inspection suitable for high-throughput sample.
Detailed description of the invention
Fig. 1 is top view illustration of the invention.Fig. 2 is the perspective view schematic diagram of Fig. 1.Fig. 3 is test paper strip schematic diagram.
Fig. 4 is specimen needle and its manipulator schematic diagram, and Fig. 5 is test paper strip storehouse schematic diagram, and Fig. 6 is the signal of NAG terminate liquid sample adding device
Figure.
In figure: 1: hydrogen peroxide agent hole；2: leukocyte esterase reagent wells；3: neuraminidase reagent wells 4: proline ammonia
Base peptase reagent wells；5: acetyl glucosaminidase reagent wells；6:PH value reagent position；7: the second conveyer belts；8: third conveying
Band；9: the four conveyer belts；10: test paper strip storehouse；11:pH value detection device；12: warm bath apparatus；13:NAG terminate liquid sample-adding dress
It sets；14: test paper strip 5-linked examines detection device；15: useless Ka Cang；16: scanning bar code device；17: sample and reagent disc；18:
Specimen needle；19: sample bit；20: reagent position；21: test paper strip；22: first conveyer belt；23: cleaning device.The electricity of 24 liftings
Cylinder, 25 servo motors rotated horizontally, the electric cylinders of 26 horizontal extensions, 27 cylinders, 28 waste liquid pools, 29 vertical electric cylinders, 30 is laterally electric
Cylinder, 31 bodies, 32 hard sample-adding pipe, 33 peristaltic pumps, 34 NAG terminate liquid reagent containers.
Specific embodiment
Attached drawing 1, Fig. 2 are a kind of specific embodiment of the invention.
Vaginal fluid detector of the invention, including the control device, sample adding device, conveying dress being mounted on body
It sets；
Sample adding device includes the sample and reagent disc 17, specimen needle 18 being mounted on body 31；Sample and reagent disc are provided with greatly
The sample bit 19 of amount and several reagent positions 20, each sample bit place a sample tube, store SNA developing solution in reagent position,
For making test paper strip " neuraminidase hole " to develop the color；Specimen needle connects body through manipulator, specimen needle for draw sample with
It is added drop-wise on the test paper strip 21 on conveyer belt after the liquid sample or developing solution stored on reagent disc；
Test paper strip is placed in conveying device and transports test paper strip runs by detecting step；Conveying device includes being mutually butted
Two traveling beds and two transverse onveyers, conveyer belt use servo motor drive control, each conveyer belt synchronous operation.
In conveying device, test paper strip storehouse 10 is arranged in the front end of longitudinal first conveyer belt 22, and test paper strip 21 is placed on
It is moved forwards in first conveyer belt, the sample or SNA developing solution that specimen needle is drawn are added drop-wise to test paper strip；The second lateral conveying
7 front end of band and first conveyer belt end on receive the test paper strip from first conveyer belt end, set at the second conveyer belt
Warm bath apparatus 12 is set, test paper strip is heated into warm bath to react；Longitudinal 8 front end of third conveyer belt and the second conveying end of tape pair
It connects, setting addition test paper strip " acetyl glucosaminidase hole " NAG terminate liquid sample adding device 13, makes above third conveyer belt
" acetyl glucosaminidase hole " terminates reaction；The front end of the 4th lateral conveyer belt 9 and third conveyer belt end on, the
Setting test paper strip 5-linked examines detection device 14 above four conveyer belts, reads color after test paper strip is reacted, output detection
As a result, useless card storehouse 15 is arranged in four end of the 4th conveyer belt, collect using the test paper strip finished.
Sample connect servo motor with 17 bottom of reagent disc and carries out driving rotation, and servo motor is mounted in body, so as to
Sample is rotated to close to the side of conveying device；The manipulator that specimen needle 18 is connected, including it is sequentially connected for going up and down
Electric cylinders 24, rotate horizontally servo motor 25, horizontal extension electric cylinders 26, wherein for lifting electric cylinders connect body, water
The electric cylinders of flattened contracting connect specimen needle, and specimen needle is using the control of cylinder 27 sucking liquid and sprays liquid；Specimen needle rotates to one
Positioning postpones decline insertion sample tube or reagent bottle, draws the SNA colour developing in the sample handled well or reagent bottle in sample tube
Then liquid is added drop-wise to the test paper strip being placed in first conveyer belt.
It is also equipped with cleaning device 23 on body, realizes that specimen needle draws the cleaning function after sample or reagent, cleaning dress
The spray head set uses spring formula spray head or waterfall type spray head, and cleaning device connects the cleaning solution in container with pump by the road；Body
On be also equipped with waste liquid pool 28, to catch the waste liquid after cleaning, waste liquid pool connects sewer through water pipe；Spray head can clean sample
On the outside of needle, specimen needle controls sucking cleaning solution using cylinder and sprays cleaning solution to clean inside.
Terminate liquid sample adding device 13 includes hard interconnected sample-adding pipe 32 and peristaltic pump 33, and sample-adding pipe connection NAG is whole
Only liquid reagent container 34.
Warm bath apparatus 12 is preferred using permanent using constant-temperature air bath device, constant temperature water bath apparatus or constant temperature oil bath device
Warm air bath apparatus；It is located at before warm bath apparatus at second conveyer belt and pH value detection device is set, test paper strip carries out pH value face
Color detection is analyzed pH value and is exported.
It is provided with scanning bar code device 16 on sample and reagent disc, scans the bar shaped in the sample tube being put into sample bit
Code.Sample bit on sample and reagent disc has 20-120, and reagent position has 2-12.
The vertical electric cylinders 29 and lateral electric cylinders 30 of cooperating, vertical electric cylinders are installed on test paper strip storehouse by connector
It is mounted on orlop, lateral electric cylinders are mounted on silo roof, and gradually elongation is horizontal to gradually push up test paper strip upwards for vertical electric cylinders
A contracting is stretched to electric cylinders one to one by one shift uppermost test paper strip in first conveyer belt onto；Conveying device runs a step, examination
Paper card storehouse just runs a step and pops up a test paper strip.
Test paper strip uses six joint inspection cards or five joint inspection cards, and six joint inspection cards are provided with hydrogen peroxide agent hole 1, leucocyte ester
Enzymatic reagent hole 2, neuraminidase reagent wells 3, Prolyl iminopeptidase reagent wells 4, acetyl glucosaminidase reagent wells 5,
6 six kinds of reagent wells of pH value reagent wells；Five joint inspection cards do not have pH value reagent wells, remaining is identical as six joint inspection cards；In the second conveyer belt
Upper warm bath heating purpose is to plant reagent, i.e. hydrogen peroxide agent hole, leukocyte esterase reagent wells, neuraminidase reagent for first five
Hole, Prolyl iminopeptidase reagent wells, the reaction of sample provides suitable reaction temperature in acetyl glucosaminidase reagent wells
Degree；Sample of the SNA developing solution for neuraminidase reagent wells in sample and reagent disc develops the color；NAG terminate liquid is for terminating
The reaction of sample in acetyl glucosaminidase reagent wells；Before being read at test paper strip 5-linked test examination instrument at the 4th conveyer belt
Color after the reaction of five kinds of reagents, analysis detection result simultaneously export.
Control device uses computer, single-chip microcontroller or PLC controller, and control device controls each servo motor, cylinder, electricity
Cylinder, scanning bar code device, peristaltic pump, warm bath apparatus, pH value detection device, test paper strip 5-linked test examination instrument.These components
It is all existing product.
PH value detection device, test paper strip 5-linked test examination instrument are generally combined referred to as vaginitis joint in the prior art
Detect work station because pH value must be detected at once plus sample, it is inaccurate to be otherwise easy result, behind five kinds of reagents need it is anti-
It could be detected after Ying Shiwu minutes, so detection device is separated, but the part of analysis data, output data is can to close
Together, it can also separate.
Vaginal fluid detector of the invention, including the sample adding device, transmission device, detection dress being mounted on body
It sets, warm bath system, strip storage device and test paper strip.
Sample adding device completes sample application, SNA developing solution sample-adding, NAG terminate liquid and is loaded function.Sample application device includes
Sample and reagent disc 17, specimen needle 18, sample bit 19, SNA reagent position, cleaning device 23, NAG terminate liquid sample adding device；SNA is aobvious
Color liquid sample adding device includes sample and reagent disc 17, reagent position 20, cleaning device 23；NAG terminate liquid sample-adding function is terminated by NAG
Liquid sample adding device 13 is realized.
Cleaning device 23 realizes that specimen needle draws the cleaning function after sample or reagent, can be used such as spring formula, waterfall type.
Cleaning device bottom end connecting line, piping connection cleaning solution.Specimen needle is made of teflon material.
Terminate liquid sample adding device uses stationary structure, connects accurate peristaltic pump and NAG terminate liquid reagent.
Scanning bar code device 16, the bar code on scanned samples pipe are provided on sample and reagent disc.It is directly connected to examine
Mechanism lis or His system is tested, it is automatic to realize detection.Lis or His system is mounted in control device or control device connection
Lis or His system.
Sample bit on sample and reagent disc has 20-120, preferably 62, and there are 2-12, preferably 6 in reagent position.
Detection device includes pH value detection device, five joint inspection detection devices (hydrogen peroxide, leukocyte esterase, sialic acid glycosides
Enzyme, Prolyl iminopeptidase, acetyl glucosaminidase) two kinds of detection devices, wherein according to the difference of test paper strip, pH
Value detection device can be matched.Device is automatically performed the color identification of test paper strip corresponding reagent position, as a result data processing exports
With computer control terminal.
Transmission device includes two traveling beds (first conveyer belt 22, third conveyer belt 8) and two transverse onveyers
(the second conveyer belt 7, the 4th conveyer belt 9), conveyer belt use band type structure.Conveyor belt type can be used such as tape conveyor belt, plate
The structures such as formula conveyer belt, screw conveyor.
Strip storage device includes test paper strip storehouse 10 and useless card storehouse 15.Strip storehouse is set to first conveyer belt front end, gives up
Card storehouse 15 is located at the end of the 4th conveyer belt.Test paper strip storehouse can store test paper strip quantity 1-200, preferred 20-200
It is a, preferably 100.Detachable or stationary structure can be used in useless Ka Cang.
Three joint inspection cards, four joint inspection cards, five joint inspection cards also can be used in test paper strip.Three joint inspection cards, four joint inspection cards, five joint inspection cards
One detection device (five joint inspection detection devices) is only set.Six joint inspection card apparatus include pH value hole detection device, five joint inspections detection
Two kinds of detection devices of device (leukocyte esterase, neuraminidase, Prolyl iminopeptidase, acetyl glucosaminidase).
Warm bath system can use constant-temperature air bath device, constant temperature water bath apparatus, constant temperature oil bath device isothermal bath mode, excellent
Choosing uses constant-temperature air bath device.Warm bath system is set to above the second conveyer belt, using air bath heating mode, temperature
35 ~ 40 DEG C of control range, 0.1 DEG C of temperature-controlled precision.
Vaginal fluid detector of the invention, first conveyer belt, the second conveyer belt, third conveyer belt, the 4th conveyer belt,
Four synchronous operations.When operation, from test paper strip storehouse 10, first test paper strip of pop-up, which is placed in first conveyer belt, is transported forward
One step of row, specimen needle 18 are drawn a certain amount of secretion sample out of a sample tube on sample and reagent disc 17, are placed on
6 reagent wells of the test paper strip in first conveyer belt, cleaning device clean specimen needle.It is aobvious that specimen needle draws a certain amount of SNA
Color liquid, is added drop-wise to the neuraminidase reagent wells of test paper strip, and cleaning device cleans specimen needle.First conveyer belt moves forwards one
Step puts second test paper strip, and sample, cleaning specimen needle is added dropwise, and SNA developing solution, cleaning specimen needle is added dropwise.First conveyer belt
A step is run further along, and the work of third test paper strip so successively carries out.Test paper strip is moved forwards by first conveyer belt
It after on conveyer belt two, is run to the right along the second conveyer belt, by reading pH value reagent on test paper strip when pH value detection device 11
The color of position 6, and carry out analysis and obtain pH value.Continue operation to the right to be heated by constant temperature bath, makes the reagent of test paper strip
It is reacted with sample.Test paper strip is run to after right end above to third conveyer belt, is then run, is passed through along third conveyer belt
Terminate liquid is added to the acetyl glucosaminidase reagent position of test paper strip when NAG terminate liquid sample adding device 13.Test paper strip
It continues to run and goes on the 4th conveyer belt, then run to the left, detect peroxide when examining detection device 14 by test paper strip 5-linked
Change hydrogen reagent position 1, leukocyte esterase reagent position 2, neuraminidase reagent position 3, general histidine amino group peptide enzymatic reagent position 4, acetyl ammonia
Color after the reaction of base glucoside enzymatic reagent position 5 analyzes output analysis result after color.Strip test paper is to the left after detection
Continue to run the useless card storehouse 15 of feeding.
Test paper strip can sequentially place several in first conveyer belt, and specimen needle 18 is often drawn from sample disk primary
As soon as after sample and time SNA developing solution, sample disk, which rotates by a certain angle, brings lower a sample tube.12 basis of warm bath apparatus
Length can be with a large amount of test paper strip placed side by side.Specimen needle according to the coordinate setting of control device come work, come draw sample,
SNA developing solution is drawn, and sample is added dropwise, SNA developing solution is added dropwise.
Claims (10)
1. a kind of vaginal fluid detector, it is characterised in that: including be mounted on sample adding device on body,
Vaginal fluid detector of the invention, it is characterised in that: including be mounted on body control device, sample adding device,
Conveying device；
Sample adding device includes the sample and reagent disc, specimen needle being mounted on body；Sample and reagent disc are provided with a large amount of sample
One's own department or unit and several reagent positions, each sample bit place a sample tube, SNA developing solution are stored in reagent position, for making test paper
Strip " neuraminidase hole " colour developing；Specimen needle connects body through manipulator, and specimen needle is deposited on sample and reagent disc for drawing
It is added drop-wise on the test paper strip on conveyer belt after the liquid sample or developing solution put；
Test paper strip is placed in conveying device and transports test paper strip runs by detecting step；Conveying device includes being mutually butted
Two traveling beds and two transverse onveyers, conveyer belt use servo motor drive control, each conveyer belt synchronous operation.
2. vaginal fluid detector according to claim 1, it is characterised in that: in conveying device, longitudinal first is defeated
Send the front end of band that test paper strip storehouse is set, to preceding operation after placement test paper strip in first conveyer belt, to connect test paper strip
By the sample or developing solution drawn from specimen needle；Lateral the second conveyer belt front end and first conveyer belt end on, connects
By the test paper strip from first conveyer belt end, warm bath apparatus is set, test paper strip carries out warm bath and adds at the second conveyer belt
Thermal response；Longitudinal third conveyer belt front end and the second conveyer belt end on, third conveyer belt top setting addition test card
Item " acetyl glucosaminidase hole " NAG terminate liquid sample adding device makes " acetyl glucosaminidase hole " to terminate reaction；It is horizontal
To the 4th conveyer belt front end and third conveyer belt end on, setting test paper strip 5-linked examines detection above the 4th conveyer belt
Device, reads color after test paper strip is reacted, output test result, and four end of the 4th conveyer belt is arranged useless Ka Cang, collects
Use the test paper strip finished.
3. vaginal fluid detector according to claim 1, it is characterised in that: sample connect servo with reagent pan bottom
Motor carries out driving rotation, and servo motor is mounted in body, to rotate to sample close to the side of conveying device；Sample
The manipulator that needle is connected, including it is sequentially connected for the electric cylinders of lifting, the servo motor of horizontal rotation, horizontal extension electricity
Cylinder, wherein the electric cylinders for lifting connect body, the electric cylinders of horizontal extension connect specimen needle, and specimen needle is using cylinder control sucking
Liquid and ejection liquid；Specimen needle rotates to decline insertion sample tube or reagent bottle after certain position, draws the place in sample tube
The SNA developing solution in sample or reagent bottle managed, is then added drop-wise to the test paper strip being placed in first conveyer belt.
4. vaginal fluid detector according to claim 1, it is characterised in that: cleaning device is also equipped on body,
Realize that specimen needle draws the cleaning function after sample or reagent, the spray head of cleaning device is sprayed using spring formula spray head or waterfall type
Head, cleaning device connects the cleaning solution in container with pump by the road, is also equipped with waste liquid pool on body, useless after cleaning to catch
Liquid, waste liquid pool connect sewer through water pipe；Spray head can clean on the outside of specimen needle, and specimen needle is using cylinder control sucking cleaning solution
Inside is cleaned with cleaning solution is sprayed.
5. vaginal fluid detector according to claim 1, it is characterised in that: terminate liquid sample adding device includes mutually interconnecting
The hard sample-adding pipe and peristaltic pump connect, sample-adding pipe connect NAG terminate liquid reagent container.
6. vaginal fluid detector according to claim 1, it is characterised in that: warm bath apparatus is filled using constant temperature air bath
It sets, constant temperature water bath apparatus or constant temperature oil bath device, preferably uses constant-temperature air bath device；It is located at warm bath at second conveyer belt to fill
It sets front and pH value detection device is set, test paper strip carries out pH value color detection, analyzes pH value and exports.
7. vaginal fluid detector according to claim 1, it is characterised in that: be provided with scanning on sample and reagent disc
Bar code device scans the bar code in the sample tube being put into sample bit；Sample bit on sample and reagent disc has 20-120
A, reagent position has 2-12.
8. vaginal fluid detector according to claim 1, it is characterised in that: pacified on test paper strip storehouse by connector
Vertical electric cylinders and lateral electric cylinders, vertical electric cylinders equipped with cooperating are mounted on orlop, and lateral electric cylinders are mounted on silo roof, vertical electricity
Gradually to gradually push up test paper strip, lateral electric cylinders one stretch a contracting to one by one by uppermost test paper to cylinder for elongation upwards
Strip is shifted onto first conveyer belt；Conveying device runs a step, and test paper strip storehouse runs a step and pops up a test paper strip.
9. vaginal fluid detector according to claim 1, it is characterised in that: test paper strip uses six joint inspection cards or five
Joint inspection card, six joint inspection cards are provided with hydrogen peroxide agent hole, leukocyte esterase reagent wells, neuraminidase reagent wells, proline
Six kinds of aminopeptidase reagent wells, acetyl glucosaminidase reagent wells, pH value reagent wells reagent wells；Five joint inspection cards do not have pH value
Reagent wells, remaining is identical as six joint inspection cards；Warm bath heating purpose is to plant reagent, i.e. hydrogen peroxide for first five on the second conveyer belt
Reagent wells, leukocyte esterase reagent wells, neuraminidase reagent wells, Prolyl iminopeptidase reagent wells, acetylglucosamine
The reaction of sample provides suitable reaction temperature in glycosides enzymatic reagent hole；SNA developing solution in sample and reagent disc is used for sialic acid
The sample in glycosides enzymatic reagent hole develops the color；NAG terminate liquid is used to terminate the reaction of sample in acetyl glucosaminidase reagent wells；?
Color after the reaction of first five kind reagent is read at 4th conveyer belt at test paper strip 5-linked test examination instrument, analysis detection result is simultaneously defeated
Out.
10. vaginal fluid detector according to claim 1, it is characterised in that: control device uses computer, single-chip microcontroller
Or PLC controller, control device control each servo motor, cylinder, electric cylinders, scanning bar code device, peristaltic pump, warm bath dress
It sets, pH value detection device, test paper strip 5-linked test examination instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910429753.7A CN110220890A (en) | 2019-05-22 | 2019-05-22 | Vaginal fluid detector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910429753.7A CN110220890A (en) | 2019-05-22 | 2019-05-22 | Vaginal fluid detector |
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| CN110220890A true CN110220890A (en) | 2019-09-10 |
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|---|---|---|---|
| CN201910429753.7A Pending CN110220890A (en) | 2019-05-22 | 2019-05-22 | Vaginal fluid detector |
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| CN110954488A (en) * | 2019-12-30 | 2020-04-03 | 长沙协大生物科技有限公司 | Vaginal secretion sample detection device and method based on color recognition |
| CN113049582A (en) * | 2021-06-02 | 2021-06-29 | 天津海关动植物与食品检测中心 | Efficient detection device and method for heavy metal ions in food |
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