CN106680479B - Multi-index integrated reagent parallel detection arbitrary combination integrator - Google Patents
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 257
- 238000001514 detection method Methods 0.000 title claims abstract description 73
- 239000012528 membrane Substances 0.000 claims abstract description 94
- 230000010354 integration Effects 0.000 claims abstract description 34
- 238000007789 sealing Methods 0.000 claims abstract description 28
- 238000005520 cutting process Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 238000001467 acupuncture Methods 0.000 claims description 10
- 238000003825 pressing Methods 0.000 claims description 5
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052782 aluminium Inorganic materials 0.000 claims description 4
- 239000011888 foil Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 238000003491 array Methods 0.000 claims 1
- 238000004458 analytical method Methods 0.000 abstract description 8
- 230000036039 immunity Effects 0.000 abstract description 6
- 230000000149 penetrating effect Effects 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 13
- 238000003860 storage Methods 0.000 description 12
- 230000006835 compression Effects 0.000 description 6
- 238000007906 compression Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000001360 synchronised effect Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract
The invention discloses a multi-index integrated reagent parallel detection random combination integrator, which comprises an integration frame, wherein an integration card carrying platform and an integration reagent box placing layer are arranged on the integration frame, an integration card is positioned on the integration card carrying platform, the integration reagent box placing layer is positioned above the integration card carrying platform, an integration reagent picking head is arranged on the integration frame and connected with an upper membrane breaker and a lower membrane breaker, the integration reagent picking head is shifted to a target acupoint of the integration reagent box along the horizontal direction and the vertical direction of the integration frame, the upper membrane breaker and the lower membrane breaker of the integration reagent picking head are matched with a sealing membrane penetrating the upper surface and the lower surface of the integration reagent box so as to enable the target integrated reagent in the integration reagent box to be loaded to a corresponding hole site of the integration card, the integration reagent corresponding to detection indexes is respectively loaded on the integration card in sequence according to the detection requirement of a patient, then the integrated reagent is processed and reacted in groups in parallel, the detection analysis of any item group is realized, and the multi-index instant detection efficiency of external luminous immunity is improved.
Description
Technical Field
The invention discloses a multi-index integrated reagent parallel detection random combination integrator, belongs to the technical field of medical instruments according to the International Patent Classification (IPC) division, and particularly relates to an integrated device for in-vitro diagnosis instant detection of multi-index item groups.
Background
In the instant detection process, the method is used for analyzing and reporting any combination multi-index results simultaneously in parallel, and has important practical significance. The multiple indexes can provide more and more accurate diagnosis information for doctors, so that the doctors can make correct diagnosis; and any multi-index combination parallel analysis can better meet the instant detection requirements of different patient conditions. In the actual detection process, the illness of each patient is different, and the test items and the quantity are different; the project group detected by each patient is used as a unit to integrate project group detection, a device capable of synchronously processing the project groups is needed to realize synchronous completion reaction, and the synchronous processing process is very important for the instant detection of the parallel report result of the project group.
The current multi-index instant detection device for whole blood samples, such as a Denmark Rate AQT90 FLEX immunoassay analyzer, bioMerieux Vidas of French Mei Liai and Mitsubishi chemical PATHFAST, is a typical multi-index instant detection device, and can realize multi-index parallel analysis and report results in a single field. The device divides other liquid components into an integrated structure of a single part in advance, and reagents are not required to be added during detection; when the equipment detects, the test index reagent of a patient is put on the machine in a group form to finish the test; however, such instruments need to begin testing the set of items of other patients after the set of indicators is tested. One project group test for each patient is generally 16-20 minutes, and the waiting time for waiting for the detection of the 2 nd patient after the 1 st patient sample enters the detection is too long, so that the efficiency is low, and the large sample treatment when more samples are used in clinic and the like is not facilitated.
The Luminex system is a multiplex detection reporting system. The system adopts a flow cell resolution technology, a fluorescence coding microsphere identification technology and an immunofluorescence labeling reporting technology, so that multiple indexes can be simultaneously detected and reported by one sampling, and more than 100 indexes can be theoretically achieved. However, due to the limited detection index of the same patient in clinical use, the compatibility of immunoreactants of each index and the complexity of instrument structure, the Luminex system is not a routine means of clinical examination. Furthermore, the samples used can be serum or plasma only, and whole blood samples cannot be used, which is not suitable for on-line detection.
Therefore, in the instant detection scheme, it is still significant to study the limited multi-index automatic parallel analysis technology suitable for clinical needs.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a multi-index integrated reagent parallel detection random combination integrator, solves the problem of random combination and integration of multi-index integrated reagents of each patient, automatically integrates and random combines the multi-index integrated reagents of each patient, enables an integrated reagent reaction unit on an integrated reagent card to process and complete reaction and detection processes in parallel, greatly shortens the report time of detection results and improves the immune detection efficiency.
In order to achieve the above purpose, the invention is realized by the following technical scheme:
The utility model provides an integrated reagent parallel detection arbitrary combination integrator, including the integration frame, be equipped with the integrated card microscope carrier on the integration frame, integrated reagent box places the layer, the integrated card of location on the integrated card microscope carrier, integrated reagent box places the layer and is located integrated card microscope carrier top, be equipped with integrated reagent pick-up head on the integration frame, integrated reagent pick-up head is connected and is gone up rupture of membranes ware and lower rupture of membranes ware, integrated reagent pick-up head shifts to the target acupuncture point of integrated reagent box along the horizontal direction and the vertical direction of integration frame, the upper rupture of membranes ware and the lower rupture of membranes ware cooperation of integrated reagent pick-up head are pierced the sealing membrane on the integrated reagent box, below so that the integrated reagent of integrated reagent box internal target is loaded to the integrated card and corresponds the hole site, the integrated reagent that the integrated reagent corresponds according to patient detection requirement is packed respectively in proper order on the integrated card realizes intelligent integration.
Further, integrated frame one side cooperation integrated kit stores the frame, and integrated kit stores and is equipped with a plurality of layers of integrated kit on the frame, and integrated kit stores and is equipped with the manipulator in frame one side and is used for taking integrated kit, and the manipulator includes flexible grab pole, and flexible grab pole is by taking motor control, flexible grab pole and take motor to install on the support, and the support bottom is unable adjustment base.
Further, a plurality of integrated reagents are sealed in the integrated reagent kit, each integrated reagent is distributed in corresponding acupuncture points of the integrated reagent kit according to a longitudinal and transverse array, sealing films are arranged on the upper portion and the lower portion of each acupuncture point to fix the corresponding integrated reagent, and the sealing films are aluminum foil vacuum sealing.
Further, a plurality of hole sites for fixing integrated reagents are arranged on the integrated card, clamping grooves are formed in two ends of the integrated card, and positioning holes are formed in the integrated card and are matched with the fixed points of the integrated card carrier for positioning.
Further, the integrated frame includes the vertical stand that the horizontal stand and the connection are connected, and the fixed integrated card carrier is connected to the vertical stand, and integrated reagent pick-up head is moved by the integrator motor drive from top to bottom in vertical direction to the integrated reagent pick-up head, and integrated reagent pick-up head is moved by the horizontal motor drive of integrator in the horizontal direction.
Further, the integrated reagent picking head slides along the horizontal guide rail and the vertical guide rail of the integrated frame, and a double-side guide rail structure is used for supporting the integrated reagent picking head to move up and down and horizontally.
Further, a picking head water Ping Mada and a picking head up-down motor are arranged on the integrated reagent picking head, the two motors adopt a stepping movement mode, the picking head horizontal motor is used for driving the integrated reagent picking head to move along the trend of the integrated reagent column of the integrated reagent kit and to be positioned above the integrated reagent in a preset sequence, and the picking head up-down motor is used for controlling an upper membrane breaker and a lower membrane breaker on the upper side and the lower side of the integrated reagent kit to move in opposite directions, so that membrane breaking and loading actions are completed, and the integrated reagent is released back to the original position.
Further, the upper membrane breaker of the integrated reagent picking head comprises a membrane breaking support, two half-structure tooth cutters are arranged at the lower end of the membrane breaking support, a tabletting support is arranged in the membrane breaking support, one end of the tabletting support is connected with a tabletting, the other end of the tabletting support is connected with a tabletting motor, the two half-structure tooth cutters are used for puncturing and cutting an upper sealing membrane of the integrated reagent kit in the descending process of the motor on the picking head, and then the tabletting is used for pushing and pressing the integrated reagent.
Further, the lower membrane breaker of the integrated reagent picking head comprises a cross cutting edge, and the cross cutting edge is used for piercing and cutting a sealing membrane at the lower part of the integrated reagent kit in the upper stroke of the lower membrane breaker and pushing the integrated reagent downwards to enter the integrated reagent clamping position for fixing under the cooperation of the upper membrane breaker.
According to the invention, the integrator loads the integrated reagents corresponding to the detection indexes on the integrated card in sequence according to the detection requirements of specific patients, so that the integrated reagents are used for loading samples at sample positions and further detection operation, and the combination and parallel detection of any item group are realized. The invention provides automatic detection flexibility, and belongs to an intelligent integration process.
The beneficial effects of the invention are as follows:
① According to the requirement of detection indexes of each patient, corresponding integrated reagents are integrated into integrated reagent cards in sequence intelligently and automatically to form any item group, and then the integrated reagent cards are processed and reacted in parallel in groups, so that detection analysis of any item group is realized, and the multi-index instant detection efficiency of in-vitro luminous immunity is improved.
② The reaction interval time between detection item groups among patients is greatly shortened, and the waiting time of the patients is greatly shortened, so that the large sample treatment in the case of more samples is facilitated.
③ The invention can realize the integration and combination reaction of any item group with multiple indexes, increases the applicable detection item range of the detection instrument, and ensures the detection and diagnosis requirements of multiple patients, multiple indexes and multiple diseases.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention.
Fig. 2 is a schematic diagram of an integrated card of the present invention.
FIG. 3 is a schematic diagram of an integrated kit of the present invention.
FIG. 4 is a schematic view of an upper membrane breaker of the integrated reagent stripper of the present invention.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
Examples: referring to fig. 1 to 4, an integrated reagent parallel detection arbitrary combination integrator comprises an integrated frame1, an integrated card carrying platform 4 and an integrated reagent box placing layer are arranged on the integrated frame1, an integrated card 12 is positioned on the integrated card carrying platform 4, the integrated reagent box placing layer is positioned above the integrated card carrying platform, an integrated reagent picking head is arranged on the integrated frame1 and is connected with an upper membrane breaker 8 and a lower membrane breaker 11, the integrated reagent picking head is shifted to a target acupoint of the integrated reagent box along the horizontal direction and the vertical direction of the integrated frame, and the upper membrane breaker and the lower membrane breaker of the integrated reagent picking head are matched with a sealing membrane 161 penetrating the upper surface and the lower surface of the integrated reagent box 16 so as to enable a target integrated reagent 17 in the integrated reagent box to be loaded to a corresponding hole site of the integrated card, and the integrated reagent 17 corresponding to detection indexes are respectively loaded on the integrated card in sequence according to the detection requirement of a patient to realize intelligent integration. The integrated kit storage rack 18 is matched with one side of the integrated rack 1, a plurality of layers of integrated kits 16 are arranged on the integrated kit storage rack 18, a manipulator is arranged on one side of the integrated kit storage rack and used for taking the integrated kit, the integrated kit storage rack and the manipulator form an integrated kit storage starting device, the manipulator comprises a telescopic grabbing rod 181, the telescopic grabbing rod is controlled by a taking motor 182, the telescopic grabbing rod and the taking motor are arranged on a bracket 183, and the bottom of the bracket is a fixed base 184. The integrated reagent kit 16 is internally provided with a plurality of integrated reagents 17 in a sealing way, each integrated reagent is distributed in corresponding acupuncture points of the integrated reagent kit according to a longitudinal and transverse array, each acupuncture point is provided with a sealing film 161 which is formed by vacuum sealing of aluminum foil for fixing the corresponding integrated reagent. The integrated card 12 is provided with a plurality of holes 122 for fixing the integrated reagent, the number of the holes can be 3-10 or more, the two ends of the integrated card are provided with clamping grooves 121, and the positioning holes are matched and positioned with the fixed points of the integrated card carrier platform, such as the integrated card fixing pins 5. The integrated card in fig. 2 shows 6 holes. The integrated frame1 comprises a horizontal frame and a vertical frame connected with the horizontal frame, the vertical frame is connected with a fixed integrated clamping carrier, the horizontal frame is connected with an integrated reagent picking head, the integrated reagent picking head is driven by an integrator up-down motor 3 in the vertical direction, and the integrated reagent picking head is driven by an integrator horizontal motor 2 in the horizontal direction. The integrated reagent picking head slides along a horizontal guide rail 6 and a vertical guide rail 7 of the integrated frame, and a double-side guide rail structure is used for supporting the integrated reagent picking head to move up and down and horizontally. The integrated reagent picking head is provided with a picking head horizontal motor 10 and a picking head up-down motor 9, the two motors adopt a stepping movement mode, the picking head horizontal motor is used for driving the integrated reagent picking head to move along the trend of the integrated reagent column of the integrated reagent kit and to be positioned above the integrated reagent in a preset sequence, and the picking head up-down motor is used for controlling the upper membrane breaker 8 and the lower membrane breaker 11 on the upper side and the lower side of the integrated reagent kit to move in opposite directions, so that membrane breaking and loading actions are completed, and the integrated reagent is released back to the original position. The upper membrane breaker 8 of the integrated reagent picking head comprises a membrane breaking support 81, a two-half-structure toothed knife 82 is arranged at the lower end part of the membrane breaking support, a tabletting support 83 is arranged in the membrane breaking support, one end of the tabletting support is connected with a tabletting 84, the other end of the tabletting support is connected with a tabletting motor 85, the two-half-structure toothed knife is used for puncturing and cutting an upper sealing membrane of the integrated reagent box in the descending process of the motor on the picking head, and the tabletting 84 is used for pushing and pressing the integrated reagent. The lower membrane breaker 11 of the integrated reagent picking head comprises a cross cutting edge, and the cross cutting edge pierces and cuts a sealing membrane at the lower part of the integrated reagent box in the upper stroke of the lower membrane breaker and pushes the integrated reagent downwards to enter the integrated reagent clamping position for fixing under the cooperation of the upper membrane breaker.
The invention provides a multi-index integrated reagent parallel detection random combination integrator, which is also called an immune detection multi-index integrated reagent random combination integrator for short, and consists of an integrated reagent box storage starting device, an integrated reagent box support (integrated frame), an integrated reagent extraction head horizontal guide rail and a vertical guide rail, an integrated card carrier, an integrated reagent extraction head horizontal motor and an integrated reagent extraction head vertical motor, an integrated reagent extraction head upper membrane breaker and an integrated reagent box lower membrane breaker, an integrated card and an integrated reagent box and the like.
An integrated kit storage and starting device for immunodetection comprises an integrated kit, an integrated kit storage bracket, an integrated reagent taking motor and a telescopic grabbing rod structure. An immunity detection integrator bracket (integration frame) comprises a horizontal structure (horizontal frame), a vertical structure (vertical frame) and an inward extending structure, wherein an integrator horizontal motor and an upper motor and a lower motor are arranged in the integrator bracket, and the integration bracket is connected and fixed with an integrated clamping platform, an integrated reagent picking head horizontal guide rail and a vertical guide rail, and is an integrator basic supporting structure. An immunity detection integrator horizontal motor and an up-down motor drive the integrator to move up-down and left-right, and support to complete the loading of integrated reagent. The utility model provides an integrated card carrier of immunodetection integrator (integrated card carrier), inwards stretches out and integrated reagent card linear track vertical position, including base, fixed pin and motion structure, the integrated card that will mark is arranged in integrated card carrier by drive structure, and fixed pin and location structure lock the integrated card, after accomplishing integrated reagent integration, fixed pin and location structure unblock, the integrated reagent card displacement of linear synchronous propeller centre gripping to sample loading position. A horizontal guide rail and a vertical guide rail of an integrated reagent picking head of an immunodetection integrator adopt a double-side guide rail structure for supporting the integrated reagent picking head to move up and down and horizontally. An immunity detection integrator integrates a reagent picking head horizontal motor and an up-down motor, and adopts a stepping movement mode. The horizontal motor is used for driving the integrated reagent picking head to move along the trend of the integrated reagent column of the integrated reagent kit and is positioned above the integrated reagent in a preset sequence. The upper motor and the lower motor are used for driving the integrated reagent picking head to move in opposite directions on the upper membrane breaker and the lower membrane breaker above and below the integrated reagent kit, so as to complete actions such as membrane breaking, loading and the like, and release the integrated reagent picking head back to the original position. An immunodetection integrator integrates an upper membrane breaker and a lower membrane breaker of a reagent picking head. The upper membrane breaker comprises a half-edge serrated knife and a middle tabletting, and is used for puncturing and cutting an upper sealing membrane of the integrated reagent in the descending process of an upper motor and a lower motor of the integrated reagent picking head, pushing the integrated reagent downwards by the tabletting, and pressing the integrated reagent downwards into the integrated card reagent position for fixing after the lower sealing membrane of the integrated reagent is broken by the lower membrane breaker. The lower membrane breaker is a cross blade, and the cross blade is used for punching and cutting the sealing membrane at the lower part of the integrated reagent on the upper side of the lower membrane breaker. An integrated card of immunity detection integrator and integrated reagent box, the integrated card has a certain number of fixed integrated reagent hole sites, and has end moving clamping groove, and there is pin positioning between 1-2 and 5-6 positions. The integrated reagent box is internally provided with sealing integrated reagents which are arranged in certain quantity vertically and horizontally, and the upper part and the lower part are all vacuum sealed by aluminum foils. The integrated reagent kit is stored and taken by the integrated reagent tube storage and taking device, and when taken, the integrated reagent tube storage and taking device pushes the integrated reagent kit to an integrated card hole site of the integrator, and the integrated reagent stripper takes the integrated reagent in a preset sequence under the synergistic action of the upper membrane breaker and the lower membrane breaker, and loads and fixes the integrated reagent in the integrated card.
After the technical scheme is adopted, under the cooperation of the storage and starting device of the immunodetection integrated kit, the integrator respectively loads the integrated reagents corresponding to the detection indexes on the integrated card in sequence according to the detection requirements of specific patients, so that the sample is loaded on the sample position and further detection operation is realized, and the combination and parallel detection of any project group are realized.
As shown in fig. 1, the integrator of the present invention mainly includes a stand (integration rack 1); an integrator horizontal motor 2; an integrator up-down motor 3; an integrated card stage 4; an integrated card fixing pin 5; an integrated reagent picking head horizontal guide rail 6; an integrated reagent picking head vertical guide rail 7; a membrane breaker 8 on the integrated reagent picking head; an integrated reagent picking head up and down motor 9; an integrated reagent picking head horizontal motor 10; an integrated reagent extraction sub-membrane breaker 11; an integrated card 12; an integrated reagent fixing hole 13; an integrated card drive motor 14; an integrated kit drive motor 15; an integrated kit 16; integrating reagent 17. These structures are combined into an integrator. The integrator loads the integrated reagents corresponding to the detection indexes on the integrated card in sequence according to the detection requirements of specific patients, so that the sample is loaded on the sample position and further detection operation is performed. Wherein, every patient detects the index differently, and the integrated reagent card of integrated combination is required according to every patient detects the index to the integrator, supplies to detect the analysis usefulness, belongs to an intelligent integrated process.
As shown in fig. 1, the ic card driving motor 14 of the present invention pushes the labeled ic card to the ic card holder 4 according to the command of the control center. The upper and lower motors 3 of the integrator push the upper and lower membrane breakers of the integrated reagent picking head to the same level of the integrated reagent box to be taken. The appointed integrated reagent is pushed to the middle position of the upper membrane breaker and the lower membrane breaker of the integrated reagent picking head under the action of the integrated reagent box driving motor, namely the integrated reagent box placement layer. The integrator horizontal motor 2 and the integrated reagent pick head horizontal motor 10 are then addressed on the integrated reagent cartridge to the integrated reagent site to be picked up and the corresponding integrated card empty acupoint or site. The integrated reagent picking head up-down motor 9 drives the integrated reagent picking head lower membrane breaker to move upwards to the position or the acupoint of the integrated reagent tube to be taken, and the integrated reagent lower sealing membrane is pierced; then the upper and lower motors 9 of the integrated reagent picking head drive the upper membrane breaker 8 of the integrated reagent picking head to continuously puncture the upper sealing membrane of the integrated reagent downwards, the compression rod (the compression support) in the upper membrane breaker 8 continuously descends, the compression plate is separated from the compression plate to compress the upper edge of the integrated reagent tube downwards under the drive of the compression driving motor of the integrated reagent picking head, and the lower membrane breaker 11 is matched with the compression plate to press the integrated reagent to the corresponding reagent hole site of the integrated card, so that the integrated reagent loading is completed. The integrated reagent membrane breaker and the integrated reagent kit are reset, and the next integrated reagent is ready to be loaded on the acupuncture point in the integrated card.
Then the upper and lower motors 3 of the integrator push the upper and lower membrane breakers of the integrated reagent picking head to the same level as the other integrated reagent box to be taken. The appointed integrated reagent is pushed to the middle position of the upper membrane breaker and the lower membrane breaker of the integrated reagent picking head under the action of the integrated reagent box driving motor. The integrator horizontal motor 2 and the integrated reagent pick head horizontal motor 10 are then addressed on the integrated reagent cartridge to the integrated reagent site to be taken and the corresponding integrated card space. The integrated reagent picking head up-down motor 9 drives the integrated reagent picking head lower membrane breaker to move upwards to the position of the integrated reagent tube to be taken, and the integrated reagent lower sealing membrane is pierced; then the upper and lower motors 9 of the integrated reagent picking head drive the upper membrane breaker 8 of the integrated reagent picking head to continuously puncture the upper sealing membrane of the integrated reagent downwards, the inner pressure rod of the upper membrane breaker 8 continuously descends, the upper edge of the integrated reagent tube is separately pressed and pressed downwards under the driving of the pressing drive motor of the integrated reagent picking head, and the lower membrane breaker 11 is matched with the integrated reagent to press the integrated reagent to the corresponding reagent hole site of the integrated card, so that the integrated reagent loading is completed. The integrated reagent membrane breaker and the integrated reagent kit are reset, and the next integrated reagent is ready to be loaded on the acupuncture point in the integrated card.
And (3) circularly loading until the integrated reagent of the required project group is completely loaded, and feeding the formed integrated reagent card to a sample loading position to load samples for detection and analysis.
After the multi-index integrated reagent parallel detection of any combination integrator completes the loading of the integrated reagent of the detection item required by one sample, the formed integrated reagent card is sent to a sample loading position for loading the sample for detection and analysis, and then the multi-index integrated reagent of the next detection sample is integrated.
Under the cooperation of the storage starting device of the immune detection integrated kit, the integrator realizes intelligent integrated reagent integration on the integrated card, and realizes combination and parallel detection of any project group.
The above description is illustrative of the embodiments using the present teachings, and is not intended to limit the scope of the present teachings to any particular modification or variation of the present teachings by those skilled in the art.
Claims (7)
1. The utility model provides an integrated ware of arbitrary combination of many index integrated reagent parallel detection which characterized in that: the integrated reagent collecting device comprises an integrated frame, wherein an integrated card carrying table and an integrated reagent box placing layer are arranged on the integrated frame, an integrated card is positioned on the integrated card carrying table, the integrated reagent box placing layer is positioned above the integrated card carrying table, an integrated reagent collecting head is arranged on the integrated frame and is connected with an upper membrane breaker and a lower membrane breaker, the integrated reagent collecting head is shifted to target acupuncture points of the integrated reagent box along the horizontal direction and the vertical direction of the integrated frame, the upper membrane breaker and the lower membrane breaker of the integrated reagent collecting head are matched with each other to puncture sealing films on the upper surface and the lower surface of the integrated reagent box so that target integrated reagents in the integrated reagent box can be loaded to corresponding hole sites of the integrated card, and the integrated reagent corresponding to detection indexes is respectively loaded on the integrated card in sequence by the integrated reagent box according to the detection requirement of a patient to realize intelligent integration;
The integrated frame comprises a horizontal frame and a vertical frame which are connected, the vertical frame is connected with a fixed integrated clamping platform, the horizontal frame is connected with an integrated reagent picking head, the integrated reagent picking head is driven by an integrator up-down motor in the vertical direction, and the integrated reagent picking head is driven by the integrator horizontal motor in the horizontal direction;
The integrated reagent picking head is provided with picking head water Ping Mada and a picking head up-down motor, the two motors adopt a stepping movement mode, the picking head horizontal motor is used for driving the integrated reagent picking head to move along the trend of the integrated reagent column of the integrated reagent kit and is positioned above the integrated reagent in a preset sequence, and the picking head up-down motor is used for controlling the upper membrane breaker and the lower membrane breaker on the upper part and the lower part of the integrated reagent kit to move in opposite directions, so that membrane breaking and loading actions are completed, and the integrated reagent is released back to the original position.
2. The multi-index integrated reagent parallel detection arbitrary combination integrator according to claim 1, wherein: the integrated frame one side cooperation integrated kit stores the frame, is equipped with a plurality of layers of integrated kit on the integrated kit stores the frame, and integrated kit stores frame one side and is equipped with the manipulator and is used for taking integrated kit.
3. The multi-index integrated reagent parallel detection arbitrary combination integrator according to claim 1, wherein: the integrated reagent kit is internally provided with a plurality of integrated reagents in a sealing mode, all the integrated reagents are distributed in corresponding acupuncture points of the integrated reagent kit according to longitudinal and transverse arrays, sealing films are arranged on the upper portion and the lower portion of each acupuncture point to fix the corresponding integrated reagents, and the sealing films are aluminum foil vacuum sealing.
4. The multi-index integrated reagent parallel detection arbitrary combination integrator according to claim 1, wherein: and a plurality of hole sites for fixing integrated reagents are arranged on the integrated card, clamping grooves are formed in two ends of the integrated card, and positioning holes are formed in the integrated card and are matched with the fixed points of the integrated card carrier for positioning.
5. The multi-index integrated reagent parallel detection arbitrary combination integrator according to claim 1, wherein: the integrated reagent picking head slides along a horizontal guide rail and a vertical guide rail of the integrated frame, and a double-side guide rail structure is used for supporting the integrated reagent picking head to move up and down and horizontally.
6. The multi-index integrated reagent parallel detection arbitrary combination integrator according to claim 1, wherein: the upper membrane breaker of the integrated reagent picking head comprises a membrane breaking support, two half-structure tooth cutters are arranged at the lower end of the membrane breaking support, a tabletting support is arranged in the membrane breaking support, one end of the tabletting support is connected with a tabletting, the other end of the tabletting support is connected with a tabletting motor, the two half-structure tooth cutters are used for puncturing and cutting an upper sealing membrane of the integrated reagent kit in the descending process of the motor on the picking head, and then the tabletting is used for pushing and pressing the integrated reagent.
7. A multi-index integrated reagent parallel detection arbitrary combination integrator according to any of claims 1 to 6, characterized in that: the lower membrane breaker of the integrated reagent picking head comprises a cross cutting edge, wherein the cross cutting edge pierces and cuts a sealing membrane at the lower part of the integrated reagent kit in the upper stroke of the lower membrane breaker and pushes the integrated reagent downwards to enter the integrated reagent clamping position for fixing under the cooperation of the upper membrane breaker.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710138126.9A CN106680479B (en) | 2017-03-09 | 2017-03-09 | Multi-index integrated reagent parallel detection arbitrary combination integrator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710138126.9A CN106680479B (en) | 2017-03-09 | 2017-03-09 | Multi-index integrated reagent parallel detection arbitrary combination integrator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106680479A CN106680479A (en) | 2017-05-17 |
| CN106680479B true CN106680479B (en) | 2024-04-26 |
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| CN2511972Y (en) * | 2001-11-28 | 2002-09-18 | 上海数康生物科技有限公司 | Capacitor biosensor for inspecting multiple biomolecules parallelly |
| CN103604939A (en) * | 2013-12-03 | 2014-02-26 | 南京医科大学第二附属医院 | Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique |
| CN105301227A (en) * | 2015-11-04 | 2016-02-03 | 徐恩良 | Parallel reaction treatment device for any project group for immunodetection |
| CN105628947A (en) * | 2016-02-02 | 2016-06-01 | 徐恩良 | Whole blood autoluminescence immunoassay point-of-care test instrument |
| CN206710437U (en) * | 2017-03-09 | 2017-12-05 | 厦门先明生物技术有限公司 | A kind of multi-index cluster is combined integrator into reagent parallel detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2511972Y (en) * | 2001-11-28 | 2002-09-18 | 上海数康生物科技有限公司 | Capacitor biosensor for inspecting multiple biomolecules parallelly |
| CN103604939A (en) * | 2013-12-03 | 2014-02-26 | 南京医科大学第二附属医院 | Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique |
| CN105301227A (en) * | 2015-11-04 | 2016-02-03 | 徐恩良 | Parallel reaction treatment device for any project group for immunodetection |
| CN105628947A (en) * | 2016-02-02 | 2016-06-01 | 徐恩良 | Whole blood autoluminescence immunoassay point-of-care test instrument |
| CN206710437U (en) * | 2017-03-09 | 2017-12-05 | 厦门先明生物技术有限公司 | A kind of multi-index cluster is combined integrator into reagent parallel detection |
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