CN212039114U

CN212039114U - Automatic solid phase extraction system

Info

Publication number: CN212039114U
Application number: CN201922439296.8U
Authority: CN
Inventors: 周郭丹玉; T·德米特; 田敬东
Original assignee: Jiangsu Dongxuan Gene Technology Co ltd
Current assignee: Jiangsu Dongxuan Gene Technology Co ltd
Priority date: 2019-12-30
Filing date: 2019-12-30
Publication date: 2020-12-01
Anticipated expiration: 2029-12-30

Abstract

The utility model discloses an automatic solid phase extraction system, which comprises a workbench (1), a sample transfer cleaning system (2) arranged on the workbench (1), a reagent distribution system (3) arranged at one side of the sample transfer cleaning system (2), a reaction device moving system (4) arranged below the reagent distribution system (3), a blocking device moving system (5) arranged below the reaction device conveying system (4) and a receiving device lifting system (6) arranged below the blocking device conveying system (5); the system transfers a sample from a sample container to a reaction column through a sample transfer and cleaning system, then the sample is conveyed to the lower part of a reagent distribution system through a reaction device moving system, and then the reagent is conveyed to the reaction column through the reagent distribution system for solid phase extraction reaction; meanwhile, the cleaning system can convey the cleaning solution to the sample container for cleaning, and then the cleaning solution is sucked through the suction pipe to clean the sample instrument and the sample conveying instrument.

Description

Automatic solid phase extraction system

Technical Field

The utility model belongs to the technical field of analytical instrument, concretely relates to automatic solid phase extraction system.

Background

Solid Phase Extraction (SPE) is a sample pretreatment technology developed from the mid-eighties; the method is developed by combining liquid-solid extraction and liquid chromatography; the method is mainly used for separating, purifying and enriching samples.

The SPE technology is based on a liquid-solid phase chromatography theory, enriches, separates and purifies a sample by adopting selective adsorption and selective elution, and is a physical extraction process comprising a liquid phase and a solid phase; it can also be viewed approximately as a simple chromatographic process.

SPE is a liquid chromatography separation principle using selective adsorption and selective elution. The more common method is to make the liquid sample solution pass through the adsorbent, retain the tested substance, then use the proper strength solvent to wash away the impurity, then use a small amount of solvent to elute the tested substance rapidly, thus achieve the purpose of rapid separation, purification and concentration; interference impurities can be selectively adsorbed, so that the measured substance flows out; or adsorbing impurities and the substance to be detected simultaneously, and then selectively eluting the substance to be detected by using a proper solvent.

The solid phase extraction in the prior art mainly comprises manual operation and robot operation, wherein the manual operation consumes time and labor, the robot operation is expensive, and after one-time solid phase extraction, instruments for conveying and storing samples in a system are cleaned.

The above problems are urgently needed to be improved and to be solved.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, an automatic solid phase extraction system is provided.

The utility model adopts the technical proposal that: the utility model provides an automatic solid phase extraction system, includes the workstation, installs the sample transfer cleaning system on the workstation, sets up the reagent distribution system in sample transfer cleaning system one side, sets up the reaction unit moving system in reagent distribution system below, sets up the blocking device moving system in reaction unit moving system below and sets up the storage device operating system in blocking device moving system below.

Wherein, be equipped with a waste liquid pond in the workstation, be special for depositing the waste liquid and use.

Preferably, the sample transfer and washing system comprises a first lifting device, a sample container connected with the first lifting device, a first manifold device arranged on the sample container, a transfer tube connected with the first manifold device, a nozzle plate connected with the transfer tube and a nozzle arranged on the nozzle plate, and the sample transfer and washing system is mounted on the workbench through the first manifold device.

Preferably, the first manifold device comprises a first manifold plate, a fixing rod mounted on the first manifold plate, a guide plate sleeved with the fixing rod, a suction pipe sleeved with the guide plate, and a cleaning system mounted on the suction pipe, and the first manifold device is connected with the transfer pipe through the suction pipe.

Wherein the first lifting device can lift and lower the sample container; during the lifting process, the depth of the suction pipe in the sample container is adjusted, so that the volume of the sample sucked by the suction pipe is determined, and the sucked sample is transferred to the nozzle through the transfer pipe.

Wherein the automatic solid phase extraction system further comprises a standard pressure regulator, and the sample suction process of the pipette is realized by regulating the pressure in the pipette through the standard pressure regulator so that the atmospheric pressure is greater than the pressure in the pipette, thereby transferring the sample from the sample container to the nozzle.

Preferably, the cleaning system includes a detergent bottle, a connection pipe connected to the detergent bottle, a transfer passage connected to the connection pipe, and a guide connected to the transfer passage, and the cleaning system is connected to the first manifold plate through the transfer passage.

Wherein, the cleaning system sucks the washing liquid in the detergent bottle through the connecting pipe, then enters the conveying channel, passes through the guider, enters the sample container for cleaning, and then just like sucking the sample, the suction pipe sucks the washing liquid in the sample container to clean the sample container, the suction pipe, the transfer pipe and the nozzle.

Wherein the cleaning system further comprises a first battery valve provided on the connection pipe for closing and opening the cleaning passage.

Preferably, the reagent dispensing system comprises a second manifold device through which the reagent dispensing system is connected to the work table, and a two-way valve mounted on the second manifold device.

Preferably, the second manifold device includes a second manifold plate, a first flow hole opened in the second manifold plate, a second flow hole provided below the first flow hole, and a distribution nozzle connected to the second flow hole, and the second manifold device is connected to the two-way valve through the first flow hole.

Wherein the reagent dispensing system further comprises reagent storage and control means commonly used in the art, consisting essentially of standard pressurized reagent bottles, secondary solenoid valves and tubing, and the primary reagent dispensing system is capable of introducing various reagents from the secondary manifold device to the dispensing nozzles.

Wherein the reagent dispensing system allows reagent to enter the first flow bore, dispense reagent through the two-way valve into the second flow bore, and dispense reagent from the second flow bore into the dispensing nozzle.

Preferably, the reaction device moving system comprises a first mover, a support cart connected with the first mover, a support connected with the support cart, and a reaction column mounted on the support, and is connected with the workbench through the first mover.

Wherein, the reaction column is provided with an opening, so that waste liquid flows out from the opening and flows into a waste liquid pool after the solid-phase extraction reaction is finished.

Preferably, the barrier device moving system includes a second mover and a barrier connected to the second mover, and the barrier device moving system is connected to the work table through the second mover.

Wherein the first and second movers are devices having moving functions, the reaction column is a container for reacting a sample with a reagent, and the stopper is for stopping waste liquid.

Preferably, the storage device lifting system comprises a second lifting device and a collector arranged on the second lifting device, and the storage device lifting system is connected with the workbench through the second lifting device.

The top surface of the stopper is set to be an inclined surface, and the two sides of the stopper are provided with water passing grooves.

The first lifting device and the second lifting device adopt a lifter structure, and the lifter can be a spring device and an air cylinder device, and can also be other devices with lifting functions.

Preferably, the distribution nozzles and the reaction columns, the reaction columns and the nozzles, the nozzles and the transfer pipes, and the transfer pipes and the suction pipes are in one-to-one correspondence and are distributed in an array.

Wherein, the distribution nozzle, the reaction column, the nozzle, the transfer tube and the suction tube are respectively provided with 96.

The utility model has the advantages that: the automatic solid phase extraction system adopts a sample transfer cleaning system and a reagent distribution system, a sample is transferred from a sample container to a first manifold device along with a suction pipe and a transfer pipe through a standard pressure regulator in the sample transfer cleaning system, then enters a reaction column through a nozzle in the first manifold device, the reaction column is conveyed to the lower part of the reagent distribution system through a reaction device moving system, the reagent is transferred from a reagent bottle to a two-way valve along with a first flow hole through a standard pressurizing reagent bottle by the reagent distribution system, then is transferred to a distribution nozzle through a second flow hole by the two-way valve, and then is dripped into the reaction column from the distribution nozzle for solid phase extraction reaction, the process is convenient and simple, and the problems of time consumption and labor consumption of manual operation and high price of a robot are solved; meanwhile, the sample transfer cleaning system comprises a cleaning system, cleaning liquid can enter the conveying pipe through the connecting pipe and then enter the sample container to be cleaned through the guider, then the suction pipe sucks the cleaning liquid in the sample container to clean the sample instrument and the sample conveying instrument, and the problem that the sample instrument and the sample conveying instrument cannot be automatically cleaned in the prior art is solved through the cleaning system.

Drawings

FIG. 1 is a schematic diagram of an automatic solid phase extraction system according to the present invention;

FIG. 2 is a schematic view of the connection of the components of FIG. 1 with the table removed;

FIG. 3 is a schematic diagram of the construction of the first manifold assembly components of the sample transfer cleaning system of FIG. 1;

FIG. 4 is a schematic diagram of the cleaning system of FIG. 3;

FIG. 5 is a schematic diagram of the construction of a second manifold assembly component of the reagent dispensing system of FIG. 1;

FIG. 6 is a schematic view of the butt structure of the nozzle and the reaction column in FIG. 2.

Detailed Description

The present invention will be further described with reference to the following specific drawings.

As shown in fig. 1-6, an automatic solid phase extraction system is provided, which comprises a worktable 1, a sample transfer and cleaning system 2 mounted on the worktable 1, a reagent distribution system 3 disposed at one side of the sample transfer and cleaning system 2, a reaction device moving system 4 disposed below the reagent distribution system 3, a blocking device moving system 5 disposed below the reaction device conveying system 4, and a receiving device lifting system 6 (see fig. 1) disposed below the blocking device conveying system 5; a waste liquid pool (not marked in the figure) is arranged in the workbench 1; the sample transfer cleaning system 2 comprises a first lifting device 21, a sample container 22 connected with the first lifting device 21, a first manifold device 23 arranged on the sample container 22, a transfer pipe 24 connected with the first manifold device 23, a nozzle plate 25 connected with the transfer pipe 24 and a nozzle 26 arranged on the nozzle plate 25, wherein the sample transfer cleaning system 2 is installed on the workbench 1 through the first manifold device 23 (see fig. 2); a standard pressure regulator (not shown) is also included in the sample transfer washing system 2; the first manifold device 23 includes a first manifold plate 231, a fixing rod 232 mounted on the first manifold plate 231, a guide plate 233 sleeved with the fixing rod 232, a suction pipe 234 sleeved with the guide plate 233, and a washing system 235 mounted on the suction pipe 234, the first manifold device 23 is connected to the transfer pipe 24 through the suction pipe 234, the washing system 235 includes a detergent bottle 2351, a connection pipe 2352 connected to the detergent bottle 2351, a transfer passage 2353 connected to the connection pipe 2352, and a guide 2354 connected to the transfer passage 2353, and the washing system 235 is connected to the first manifold plate 231 through the transfer passage 2353 (see fig. 2, 3, and 4); the cleaning system 235 further includes a first battery valve disposed on the connection tube 2352 (not identified in the figures); the reagent distribution system 3 comprises a second manifold device 31 and a two-way valve 32 mounted on the second manifold device 31, the distribution system 3 being connected to the work table 1 through the second manifold device 31 (see fig. 2); the reagent dispensing system 3 further comprises a standard pressurized reagent bottle, a pipeline connected with the standard pressurized reagent bottle and a second electromagnetic valve (not shown in the figure) arranged on the pipeline; the second manifold device 31 includes a second manifold plate 311, a first flow-through hole 312 opened in the second manifold plate 311, a second flow-through hole 313 provided below the first flow-through hole 312, and a distribution nozzle 314 connected to the second flow-through hole 313, the second manifold device 31 being connected to the two-way valve 32 through the first flow-through hole 312 (see fig. 5); the reaction device moving system 4 includes a first mover 41, a support cart 42 connected to the first mover 41, a support 43 connected to the support cart 42, and a reaction column 44 mounted on the support, and the reaction device moving system 4 is connected to the table 1 through the first mover 41 (see fig. 1, 2, and 6); the barrier device moving system 5 comprises a second mover 51 and a barrier 52 connected with the second mover 51, and the barrier device moving system 5 is connected with the workbench 1 through the second mover 51 (see fig. 1 and 2); the storage device lifting system 6 includes a second lifting device 61 and a collector 62 disposed on the second lifting device 61, and the storage device lifting system 6 is connected to the work table 1 through the second lifting device 61 (see fig. 1).

The utility model discloses a theory of operation: the automatic solid phase extraction system is very convenient to use, when solid phase extraction is needed, the system is started, the first lifting device 21 in the sample transfer cleaning system 2 on the worktable 1 is started in advance, the first elevating means 21 elevates, according to the volume of the sample to be extracted, the sample container 22 to a specific position, the pipette 234 is driven into the sample to a depth just equal to the volume of the sample to be reacted, and then the sample is aspirated from the sample container 22 by adjusting the pressure appropriately through the standard pressure regulator, the sample is transported through the pipette 234 to the transfer tube 24, from the transfer tube 24 to the first manifold device 23, and from the first manifold device 23 to the nozzle 26, whereupon, the reaction column 44 on the reaction device moving system 4 is just below the first manifold device 23, and the reaction columns 44 correspond one-to-one to the nozzles 26 so that the sample is just transferred to the reaction columns 44.

After the sample is transferred through the above process, the reaction column 44 moves on the first mover 41 in the reaction device transportation system 4 to move under the reagent distribution system 3, and at the same time, the stopper 52 moves on the second mover 51 in the stopper movement system 5 to move under the reaction column 44, at this time, the reagent distribution system 3 is activated to open the second electromagnetic valve, at this time, under the action of the standard pressurized reagent bottle, the reagent enters the first flow hole 312 of the second manifold plate 311 through the pipeline, enters the two-way valve 32 through the first flow hole 312, sends the reagent into the second flow hole 313 through the two-way valve 32, enters the distribution nozzle 314 through the second flow hole 313, and the reagent enters the reaction column 44 from the distribution nozzle 314 to perform the solid phase extraction reaction with the sample.

The reaction column 44 is used for performing solid phase extraction, simultaneously, the cleaning system 235 in the sample transfer cleaning system 2 is started, the first electromagnetic valve is opened, the cleaning solution is output from the detergent bottle 2351, enters the conveying channel 2353 through the connecting pipe 2352, is conveyed into the sample container 22 through the guider 2354 for cleaning, then flows through the process of transferring the sample by the sample transfer cleaning system 2, flows in the suction pipe 234, the transfer pipe 24 and the nozzle 26 and is cleaned, and finally the effluent waste liquid is conveyed into the waste liquid pool of the workbench 1, so that the problem that the sample instrument and the sample conveying instrument cannot be automatically cleaned in the prior art is solved.

After the reaction column 44 is subjected to the solid-phase extraction reaction, the opening of the reaction column 44 is opened, the waste liquid flows out from the opening, and flows onto the stopper 52, flows into the waste liquid pool along with the inclined surface of the stopper 52 and the water passing groove, the waste liquid is completely flowed, the stopper 52 moves to the lower part of the first manifold device 23 along with the second mover 51, at the moment, the storage device lifting system 6 is started, the second lifting device 61 starts to ascend, meanwhile, the collector 62 contacts the reaction column 44, and finished products in the reaction column 44 are collected.

Through the process, the automatic solid phase extraction system is very convenient to use and is manufactured through simple equipment, and the problems of time and labor consumption of manual operation and high price of a robot are solved.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above, it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. An automatic solid phase extraction system, which is characterized in that: the device comprises a workbench (1), a sample transfer and cleaning system (2) arranged on the workbench (1), a reagent distribution system (3) arranged on one side of the sample transfer and cleaning system (2), a reaction device moving system (4) arranged below the reagent distribution system (3), a blocking device moving system (5) arranged below the reaction device moving system (4) and a receiving device lifting system (6) arranged below the blocking device moving system (5).

2. An automated solid phase extraction system according to claim 1, wherein: the sample transfer and cleaning system (2) comprises a first lifting device (21), a sample container (22) connected with the first lifting device (21), a first manifold device (23) arranged on the sample container (22), a transfer pipe (24) connected with the first manifold device (23), a nozzle plate (25) connected with the transfer pipe (24) and a nozzle (26) arranged on the nozzle plate (25), wherein the sample transfer and cleaning system (2) is arranged on the workbench (1) through the first manifold device (23).

3. An automated solid phase extraction system according to claim 2, wherein: the first manifold device (23) comprises a first manifold plate (231), a fixing rod (232) mounted on the first manifold plate (231), a guide plate (233) sleeved with the fixing rod (232), a suction pipe (234) sleeved with the guide plate (233) and a cleaning system (235) mounted on the suction pipe (234), and the first manifold device (23) is connected with the transfer pipe (24) through the suction pipe (234).

4. An automated solid phase extraction system according to claim 3, wherein: the cleaning system (235) comprises a detergent bottle (2351), a connecting pipe (2352) connected with the detergent bottle (2351), a conveying channel (2353) connected with the connecting pipe (2352) and a guide (2354) connected with the conveying channel (2353), wherein the cleaning system (235) is connected with the first manifold plate (231) through the conveying channel (2353).

5. An automated solid phase extraction system according to claim 4, wherein: the reagent distribution system (3) comprises a second manifold device (31) and a two-way valve (32) mounted on the second manifold device (31), and the reagent distribution system (3) is connected with the workbench (1) through the second manifold device (31).

6. An automated solid phase extraction system according to claim 5, wherein: the second manifold device (31) comprises a second manifold plate (311), a first through hole (312) formed in the second manifold plate (311), a second through hole (313) formed below the first through hole (312), and a distribution nozzle (314) connected to the second through hole (313), and the second manifold device (31) is connected to the two-way valve (32) through the first through hole (312).

7. An automated solid phase extraction system according to claim 6, wherein: the reaction device moving system (4) comprises a first moving device (41), a support trolley (42) connected with the first moving device (41), a support (43) connected with the support trolley (42) and a reaction column (44) installed on the support (43), and the reaction device moving system (4) is connected with the workbench (1) through the first moving device (41).

8. An automated solid phase extraction system according to claim 7, wherein: the barrier device moving system (5) comprises a second mover (51) and a barrier (52) connected with the second mover (51), and the barrier device moving system (5) is connected with the workbench (1) through the second mover (51).

9. An automated solid phase extraction system according to claim 8, wherein: the storage device lifting system (6) comprises a second lifting device (61) and a collector (62) arranged on the second lifting device (61), and the storage device lifting system (6) is connected with the workbench (1) through the second lifting device (61).

10. An automated solid phase extraction system according to claim 9, wherein: the distribution nozzles (314) and the reaction columns (44), the reaction columns (44) and the nozzles (26), the nozzles (26) and the transfer pipes (24), and the transfer pipes (24) and the suction pipes (234) are in one-to-one correspondence and are distributed in an array.