CN212159315U

CN212159315U - Device for automatically enriching red tide toxins in concentrated seawater

Info

Publication number: CN212159315U
Application number: CN202020540921.8U
Authority: CN
Inventors: 史倩; 曹煊; 张颖颖
Original assignee: Oceanographic Instrumentation Research Institute Shandong Academy of Sciences
Current assignee: Oceanographic Instrumentation Research Institute Shandong Academy of Sciences; Institute of Oceanographic Instrumentation Shandong Academy of Sciences
Priority date: 2020-04-14
Filing date: 2020-04-14
Publication date: 2020-12-15
Anticipated expiration: 2030-04-14

Abstract

The utility model relates to a marine environment monitoring technology field, concretely relates to marine organic pollutant's detection device. An apparatus for automatically enriching red tide toxins in concentrated seawater, comprising: a sample introduction device, an extraction device and a collection device; the sample introduction device comprises a plurality of sample bottles and a multi-channel selection valve; the sample bottle is filled with a sample to be detected and a reagent; the sample bottle is connected with the multi-channel selection valve and the multi-channel selection valve is connected with the extraction device through pipelines, and a sample to be detected and different reagents are added into the extraction device in sequence through the multi-channel selection valve; the liquid outlet of the extraction device enters a collection device through a pipeline. The utility model is an automatic enrichment device, which does not need manual operation, and saves manpower, material resources and time; the solid phase extraction and the nitrogen blowing device are combined together, so that the enrichment and concentration of the sample can be completed at one time, and the sample does not need to be transferred after the enrichment is finished, thereby causing secondary pollution; the device has reasonable structural design and is convenient to maintain and dismantle and repair.

Description

Device for automatically enriching red tide toxins in concentrated seawater

Technical Field

The utility model relates to a marine environment monitoring technology field relates to marine organic pollutant's detection device.

Background

In recent years, the increasingly common and frequent red tide phenomenon seriously damages the balance of a marine ecosystem, greatly damages fishery resources and marine aquaculture, and forms a direct threat to the health level and living environment of human beings.

Because the content of the red tide toxin in the seawater can be as low as ng/L order of magnitude, the seawater has complex substrate and higher salinity, the sample pretreatment is required before the detection, so as to reduce the existence of interferents as much as possible, realize the extraction and enrichment of the sample and improve the detection sensitivity. However, the conventional laboratory red tide toxin pretreatment technology has the following problems:

firstly, a large amount of seawater needs to be filtered, secondly, a solid phase extraction column (SPE) is used for extraction, different reagents are selected again to elute the red tide toxins adsorbed on the SPE, and finally, the red tide toxins are sent to a high performance liquid chromatography-mass spectrometer for detection through nitrogen blowing concentration. The pretreatment process of the whole sample needs manual work, the process is complex and time-consuming, cross contamination is easily caused, field and efficient operation cannot be realized, and the detection result is inaccurate.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a solve the problem that present sea water red tide toxin pretreatment method process is loaded down with trivial details, consuming time and wasting force, propose an automatic enrichment facility and method that can be applied to on-the-spot or laboratory's high efficiency, quick sea water red tide toxin, can save manual work and time cost greatly, improve the efficiency of pretreatment.

In order to solve the technical problem, the utility model discloses a following technical scheme realizes:

an apparatus for automatically enriching red tide toxins in concentrated seawater, comprising: a sample introduction device, an extraction device and a collection device; the sample introduction device comprises a plurality of sample bottles and a multi-channel selection valve; the sample bottle is filled with a sample to be detected and a reagent; the sample bottle is connected with the multi-channel selection valve and the multi-channel selection valve is connected with the extraction device through pipelines, and a sample to be detected and different reagents are added into the extraction device in sequence through the multi-channel selection valve; the liquid outlet of the extraction device enters a collection device through a pipeline.

Further preferably, the extraction device is connected with a peristaltic pump, and the sample or the reagent enters the extraction device through a pipeline under the action of the peristaltic pump.

Further preferably, the peristaltic pump is connected with an isolation valve, and the isolation valve is connected with a collection device; the collection device includes a sample collection bottle and a waste bottle.

Further preferably, the device also comprises a nitrogen blowing device for drying the collected red tide toxin, wherein the nitrogen blowing device comprises a nitrogen bottle, an 1/4-inch copper tube connected with the nitrogen bottle and a nitrogen blowing needle; the nitrogen blowing needle is introduced into the sample collecting bottle; and a pressure reducing valve is arranged at the mouth of the nitrogen cylinder.

Further preferably, a filter is arranged in a sample bottle for containing the seawater to be detected; the filter is a needle filter with a glass fiber filter membrane with the aperture of 0.45 mu m, one end of the filter is directly connected with seawater, and the other end of the filter is connected with a sample inlet pipe of the multi-channel selection valve.

Further preferably, the device also comprises a control circuit board, and the control circuit board is used for controlling the rotation of the multi-channel selection valve, the starting/closing of the peristaltic pump, the rotating speed, the switching of the isolating valve passage and the switch of the nitrogen blowing device.

Compared with the prior art, the utility model discloses following beneficial effect has:

1. the utility model is an automatic enrichment device, which does not need manual operation, and saves manpower, material resources and time;

2. the device of the utility model combines the solid phase extraction device and the nitrogen blowing device together, can complete the enrichment and concentration of the sample at one time, and does not need to transfer the sample after the enrichment and causes secondary pollution;

3. the device of the utility model has the advantages of reasonable design, convenient maintenance, overhaul.

Drawings

Fig. 1 is a schematic overall structure diagram of an apparatus for automatically enriching red tide toxins in concentrated seawater according to an embodiment of the present invention;

fig. 2 is a front view of fig. 1.

Detailed Description

In order to facilitate understanding of the invention, the invention is described in more detail below with reference to the accompanying drawings and specific embodiments. Preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

The utility model provides one of them embodiment is: an automatic enrichment and concentration device for red tide toxins in seawater mainly comprises a base 1, a filter 2, a multi-channel selection valve 3, a sample bottle 4, a nitrogen bottle 5, a pressure reducing valve 6, an 1/4-inch copper tube suite 7, a nitrogen blowing needle 8, a solid phase extraction column 9, a peristaltic pump 10, an isolation valve 11, a sample collecting bottle 12, a waste liquid bottle 13, a polytetrafluoroethylene conduit 14, a nitrogen blowing device and a control circuit board.

Base 1 is the stainless steel towards the orifice plate, and multichannel selection valve 3, solid-

phase extraction post

9, 1/4 inches copper pipe external member 7, peristaltic pump 10, isolating valve 11 all install in the front of base 1, and the bottom of base 1 is equipped with load-bearing platform, is equipped with a N bottle seat on this load-bearing platform, and sample bottle 4 installs in the bottle seat. Methanol, ultrapure water, a seawater sample, a 15% methanol aqueous solution, and a methanol solution containing 1% ammonia water are contained in 5 sample bottles 4, respectively. The sample bottles are respectively connected with 1, 2, 3, 4 and 6 valve ports of a multi-channel selection valve 3 (a six-channel selection valve in the embodiment) through polytetrafluoroethylene guide pipes 14, and the polytetrafluoroethylene guide pipes of the 5 valve ports are directly connected with air or are placed in the empty sample bottles. The multichannel selection valve 3 selects the reagent of the designated channel by rotating. Wherein, a filter 2 for filtering impurities in water is arranged in front of the conduit communicated with the seawater sample. The filter 2 is a needle filter with a glass fiber filter membrane with the aperture of 0.45 mu m, one end is directly connected with seawater, and the other end is connected with a polytetrafluoroethylene conduit by a rubber tube and is led into a 3-position valve port of the multi-channel selection valve 3.

The multi-channel selection valve 3 is connected with the solid phase extraction column 9 through a polytetrafluoroethylene conduit 14, and the bottom of the solid phase extraction column 9 is connected with the peristaltic pump 10 through the polytetrafluoroethylene conduit 14. The peristaltic pump 10 is provided with a peristaltic pump pipe, and the seawater sample to be detected, the required reagent and air are sequentially extracted from the solid-phase extraction column 9 through the peristaltic pump 10. The solid phase extraction column 9 is an Oasis HLB solid phase extraction column of Waters.

The peristaltic pump tube of the peristaltic pump 10 is connected with an isolation valve 11 through a polytetrafluoroethylene conduit 14, and the isolation valve 11 is connected with a sample collection bottle 12 and a waste liquid bottle 13 through the polytetrafluoroethylene conduit. The isolation valve 11 is a three-channel isolation valve, each channel is provided with a joint, a hoop and a polytetrafluoroethylene conduit, and the two channels can be switched at will to shunt the solution eluted from the solid-phase extraction column 9 and enter a sample collection bottle or be discharged into a waste liquid bottle.

The nitrogen blowing device consists of a nitrogen gas bottle 5, a pressure reducing valve 6, an 1/4-inch copper tube sleeve 7 and a nitrogen blowing needle 8. The nitrogen gas cylinder 5 is connected to an 1/4-inch copper tube set 7, and the 1/4-inch copper tube set 7 is fixed to the base 1, and its lower end is connected to a nitrogen blow pin 8, which extends into the sample collection bottle 12. The pressure reducing valve 6 is installed at the mouth of the nitrogen gas cylinder 5.

On the base 1, a control circuit board is also mounted. The control circuit board is used for controlling the rotation of the multi-channel selection valve, the starting/closing of the peristaltic pump, the rotating speed, the switching of the isolating valve access and the like.

The working principle and the process of the device for automatically enriching red tide toxins in concentrated seawater of the utility model are described as follows:

(1) starting a multi-channel selection valve, rotating a valve port to 1 position, rotating an isolation valve to a waste liquid bottle channel, starting a peristaltic pump, setting the pump speed to be 1 mL/min, extracting a methanol solution, and closing the peristaltic pump after 5 mL is extracted;

(2) turning the valve port to 2 positions, starting the peristaltic pump, and closing the peristaltic pump after 5 mL of ultrapure water is pumped;

(3) turning the valve port to 3 positions, starting the peristaltic pump, pumping 300 mL of the filtered seawater sample to be detected, and closing the peristaltic pump;

(4) turning the valve port to 4 positions, starting a peristaltic pump, pumping 5 mL of 15% methanol aqueous solution, and closing the peristaltic pump;

(5) turning the valve port to 5 positions, starting the peristaltic pump, pumping air for 5 min, and closing the peristaltic pump;

(6) turning the valve port to 6 positions, switching the isolating valve to a sample collecting bottle channel, starting the peristaltic pump, pumping 15 mL of methanol solution containing 1% ammonia water, and closing the peristaltic pump;

(7) and opening the nitrogen blowing device, and drying the sample in the sample collection bottle to obtain the enriched and concentrated red tide toxin sample.

Claims

1. The utility model provides an automatic device of red tide toxin in enrichment sea water which characterized in that: comprises a sample introduction device, an extraction device and a collection device; the sample introduction device comprises a plurality of sample bottles and a multi-channel selection valve; the sample bottle is filled with a sample to be detected and a reagent; the sample bottle is connected with the multi-channel selection valve and the multi-channel selection valve is connected with the extraction device through pipelines, and a sample to be detected and different reagents are added into the extraction device in sequence through the multi-channel selection valve; the liquid outlet of the extraction device enters a collection device through a pipeline.

2. The apparatus for automatically enriching red tide toxins in concentrated seawater according to claim 1, wherein: the extraction device is connected with a peristaltic pump, and a sample or a reagent enters the extraction device through a pipeline under the pumping action of the peristaltic pump.

3. The apparatus for automatically enriching red tide toxins in concentrated seawater according to claim 2, wherein: the peristaltic pump is connected with an isolation valve, and the isolation valve is connected with a collecting device; the collection device includes a sample collection bottle and a waste bottle.

4. The apparatus for automatically enriching red tide toxins in concentrated seawater according to claim 3, wherein: the device also comprises a nitrogen blowing device for drying the collected red tide toxin, and the nitrogen blowing device comprises a nitrogen bottle, an 1/4-inch copper tube connected with the nitrogen bottle and a nitrogen blowing needle; the nitrogen blowing needle is introduced into the sample collecting bottle; and a pressure reducing valve is arranged at the mouth of the nitrogen cylinder.

5. The apparatus for automatically enriching red tide toxins in concentrated seawater according to claim 1, wherein: a filter is arranged in the sample bottle for containing the seawater to be detected.

6. The apparatus for automatically enriching red tide toxins in concentrated seawater as claimed in claim 5, wherein: the filter is a needle filter, one end of the filter is directly connected with seawater, and the other end of the filter is connected with a sample inlet pipe of the multi-channel selection valve.

7. The apparatus for automatically enriching red tide toxins in concentrated seawater as claimed in claim 4, wherein: the control circuit board is used for controlling the rotation of the multi-channel selection valve, the starting/closing of the peristaltic pump, the rotating speed, the switching of the isolating valve passage and the switching of the nitrogen blowing device.