CN209210815U - Integrated processing system before sequencing - Google Patents

Abstract

The utility model provides an integrated processing system before sequencing, which includes a first module for executing the first processing process of sequencing samples, a second module for executing the second processing process, and an air circulation system. The first module and the second module are adjacently arranged and The internal processing space is communicated with an opening. The air circulation system includes an air intake fan located in the first module and an air outlet fan located in the second module. The air intake fan introduces external air into the processing space of the first module, making the first module Positive pressure is formed in the processing space, and the air outlet fan discharges the air in the processing space of the second module to form a negative pressure in the processing space of the second module. The pressure difference between the processing space of the first module and the second module makes the air flow from the first One module enters the second module and is discharged by the air outlet fan. The utility model also provides another pre-sequencing integrated processing system. The system also includes an intermediate module arranged between the first module and the second module. The utility model has a high degree of integration and reduces pollution.

Description

Integrated processing system before sequencing

technical field

The utility model relates to the field of gene sequencing, in particular to an integrated processing system before gene sequencing.

Background technique

PCR (polymerase chain reaction) technology has high specificity, selectivity and sensitivity, and is fast, simple and easy to automate, so it has been widely valued and reasonably applied in clinical medicine. At present, it can be applied smoothly in many primary medical units in our country. Due to the extremely high sensitivity of PCR, certain specific sequences in the template can be amplified to millions of times within a few hours, and the amplified products may contaminate the pipette, operating table or The aerosol formed by evaporation will pollute the entire laboratory. Once pollution occurs, it is extremely difficult to eliminate the source of pollution, and it is often necessary to thoroughly clean the entire laboratory equipment.

This kind of PCR pollution includes template DNA pollution and primer pollution; macroscopically, it can be divided into two categories: one is room pollution, including air pollution, mutual flow of staff, pollution caused by equipment and reagent transfer and cross-use; The first category is indoor pollution, including pollution caused by unscrupulous operations, incomplete disinfection, and unqualified reagents. At present, the prevention of PCR pollution mainly includes the following aspects: 1. Install a laminar flow workbench with a UV lamp, place disposable gloves, a complete set of pipettes and other necessities in the workbench, and turn on the UV lamp after each operation Disinfection; 2. Various test tubes, droppers, tips, etc. must be autoclaved before use, and must not be reused; 3. Support special reagents to minimize repeated freezing and thawing of reagents; 4. Add samples quickly to avoid gas Sol.

Currently, sequencing sample processing and library preparation are generally carried out in different workshops: 1. Specimen processing area, including the preparation of amplification templates; 2. PCR amplification area, including the preparation of reaction solutions and PCR amplification; 3. Product analysis area, gel electrophoresis analysis, product photography and preparation of recombinant clones. Among them, the former treatment and the latter treatment need to be carried out in different isolation areas, and the airflow direction must have certain fluidity, from sample preparation→PCR (polymerase chain reaction) amplification→product analysis→product processing into and out of the workshop All have airflow buffers, and the direction of airflow needs to be controlled to prevent cross-contamination of aerosols in each workshop. This method needs to put each functional module in an independent work room, so that the equipment integration is not high, it needs to occupy more space and more operators, the work efficiency is not high, and the maintenance of the equipment is also inconvenient. The cost of room construction is also high.

Utility model content

In view of this, it is necessary to provide an integrated pre-sequencing processing system to solve the above problems.

In one aspect, an integrated pre-sequencing processing system is provided, including a first module for performing a first processing procedure of a sequencing sample, a second module for performing a second processing procedure of a sequencing sample, and an air circulation system, the first module It is adjacent to the second module and has a processing space inside. The processing spaces of the first module and the second module have openings to communicate with each other. The air circulation system includes an air inlet arranged on the first module. The fan and the outlet fan arranged on the second module, the inlet fan introduces external air into the processing space of the first module, so that a positive pressure is formed in the processing space of the first module, and the outlet fan The blower exhausts the air in the processing space of the second module from the integrated processing system before sequencing, so that a negative pressure is formed in the processing space of the second module, and the processing space of the first module and the processing space of the second module The pressure difference between them makes the air flow enter the processing space of the second module from the processing space of the first module, and the integrated system before sequencing is exhausted by the air outlet fan, thereby exhausting the first module and the second module. The second module treats pollutants in the space.

Further, the air inlet fan is arranged on the top of the first module, and/or the outlet fan is arranged on the top of the second module.

Further, the air inlet fan is provided with a filter to filter the air entering the first module, and/or the outlet fan is provided with a filter to filter the air discharged from the pre-sequencing integrated processing system.

Further, the first module and the second module transfer the sequencing samples from the first module to the second module through a transfer device.

On the other hand, there is also provided an integrated pre-sequencing processing system, including a first module for executing the first processing procedure of the sequencing sample, a second module for executing the second processing procedure of the sequencing sample, and performing the processing between the first processing procedure and the second The intermediate module and the air circulation system of the intermediate process between the processing processes, the first module, the intermediate module and the second module are all provided with a processing space inside, the first module and the intermediate module are adjacent to each other and the processing space The second module of the intermediate module is arranged adjacently and has an opening communication between the processing spaces. The air circulation system includes an air intake fan arranged on the first module and a fan arranged on the second module. An air outlet fan, the air inlet fan introduces external air into the processing space of the first module to form a positive pressure in the processing space of the first module, and the air outlet fan introduces external air into the processing space of the second module The air discharged from the pre-sequencing integrated processing system creates a negative pressure in the processing space of the second module, and the pressure difference between the processing space of the first module and the second module makes the air flow from the processing space of the first module enter the processing space of the middle module, then enter the processing space of the second module from the middle module, and finally discharge the pre-sequencing integrated system by the air outlet fan, so as to discharge the second The first module, the middle module and the second module treat pollutants in the space.

Further, the air circulation system also includes a middle air inlet fan arranged in the middle module, and the middle air inlet fan introduces external air into the processing space of the middle module, so that the processing space of the middle module forms A positive pressure that is lower than the positive pressure in the process space of the first module.

Further, the number of the intermediate modules is one or more.

Further, an air deflector is provided on the intermediate module, and the air deflector is arranged facing the air inlet of the intermediate module, and the air deflector directs the external air introduced by the intermediate air inlet fan to the position of the second module. .

Further, the first module and the intermediate module transfer the sequencing sample from the first module to the intermediate module through the transfer device; and the intermediate module and the second module transfer the sequencing sample from the intermediate module through the transfer device transfer of a module to said second module.

Further, the first module is a centrifugation module, the intermediate module is a sample processing module, and the second module is a library preparation module.

The pre-sequencing integrated processing system provided by the utility model integrates two or more modules that process samples before sequencing to form a pre-sequencing integrated processing system, which improves the integration of equipment and effectively reduces the floor area and personnel configuration. , greatly improving data output. In addition, the pre-sequencing integrated processing system is also equipped with an air circulation system for air circulation and airflow guidance, so that the generated aerosol and other pollution sources are discharged from the integrated system, reducing pollution.

Description of drawings

FIG. 1 is an overall schematic diagram of the pre-sequencing integrated processing system in the first embodiment of the present invention.

Fig. 2 is an exploded schematic diagram of the pre-sequencing integrated processing system shown in Fig. 1 .

FIG. 3 is a schematic view of the airflow direction of the air circulation system shown in FIG. 2 .

Fig. 4 is an overall schematic diagram of the pre-sequencing integrated processing system in the second embodiment.

Fig. 5 is a schematic layout diagram of the pre-sequencing integrated processing system described in Fig. 4 .

FIG. 6 is a schematic diagram of the airflow direction of the pre-sequencing integrated processing system described in FIG. 4 .

The following specific embodiments will further illustrate the utility model in conjunction with the above-mentioned accompanying drawings.

Description of main component symbols

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

It should be noted that when a component is said to be "mounted on" another component, it may be directly on the other component or there may be an intervening component. When a component is said to be "set on" another component, it may be set directly on the other component or there may be an intervening component at the same time.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of this invention. The terminology used in the description of the utility model herein is only for the purpose of describing specific embodiments, and is not intended to limit the utility model. As used herein, the term "or/and" includes any and all combinations of one or more of the associated listed items.

It should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer" and so on are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the embodiment of the present utility model and simplified descriptions, rather than indicating or implying that the device or component referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the utility model. The terms "first" and "second" are only used to distinguish objects, and do not refer to a specific sequence relationship.

Please refer to FIG. 1-FIG. 3, which are the overall schematic diagrams of the pre-sequencing integrated processing system in the first embodiment. In this embodiment, the pre-sequencing integrated processing system 1000 is an integrated system for sequencing sample processing and library preparation, including a first module 100, a second module 200, and an intermediate module between the first module 100 and the second module 200. The module 300 , and the air circulation system 400 arranged on the first module 100 , the middle module 300 and the second module 200 . Wherein, the pre-sequencing integrated processing system 1000 involves processing the three processing procedures that the sequencing samples need to go through sequentially before sequencing, wherein the first module 100 , the intermediate module 300 and the second module 200 respectively execute one of the processing procedures in sequence. The first module 100 , the intermediate module 300 and the second module 200 are integrated into one piece. The integration means that the three are close in position, centralized in function, and/or share related components. The first module 100, the middle module 300, and the second module 200 all have a processing space inside, and the first module 100 executes the first processing process of the sequencing sample in its processing space, the middle module 300 is set adjacent to the first module 100, and the middle The module 300 executes the intermediate processing process of the sequencing sample in its processing space, and a transmission device (not shown in the figure), such as a conveyor belt, is arranged between the first module 100 and the intermediate module 300, and the sequencing sample processed by the first module 100 is transmitted through the conveyor belt. The intermediate processing is performed for the intermediate module 300 . The second module 200 is disposed adjacent to the middle module 300, and the second module 200 executes a second processing process in its processing space. A transmission device (not shown in the figure), such as a conveyor belt, is provided between the intermediate module 300 and the second module 200, through which the sequencing samples processed by the intermediate module 300 are transmitted to the second module 200 to perform the second processing process. The first module 100 is provided with a channel for sequencing samples to be placed in, such as setting an openable and closable door for sequencing samples to be placed in, and the second module 200 is provided with a channel for sequencing samples to be removed, such as setting an openable door for sequencing samples to be removed closed door etc. Of course, the channels on the first module 100 and the second module 200 can also be used for other purposes. In addition, for some other purposes, passages such as doors that can be opened and closed may also be provided on the intermediate module 300 . After the sequencing sample is placed in the first module 100, the first module 100 performs the first processing process, and then is transported to the intermediate module 300 by the conveyor belt, and the intermediate module 300 performs the intermediate processing process, and then is transmitted to the second module 200 by the conveyor belt Executing the second processing process, the sequencing samples that have completed the first, second, and third processing processes in this order are removed, and continue to be processed before sequencing or directly sequenced.

The air circulation system 400 is placed above the pre-sequencing integrated processing system 1000 , specifically, the air circulation system 400 is placed above the first module 100 , the middle module 300 and the second module 200 . Wherein, an inlet fan 410 is arranged above the first module 100 , an intermediate inlet fan 420 is arranged above the middle module 300 , and an outlet fan 430 is arranged above the second module 200 . The air inlet fan 410 is arranged at the air inlet 101 opened above the first module 100, and is used to introduce external air into the first module 100 through the air inlet 101, and the middle air inlet fan 420 is arranged at the air inlet 301 opened above the middle module 300 is used to introduce external air into the middle module 300 through the air inlet 301 . The power of the inlet fan 410 is higher than that of the middle inlet fan 420, and the inlet fan 410 introduces relatively more air into the first module 100, so that the first module 100 presents a relatively high positive pressure. The middle air intake fan 420 introduces relatively little air into the middle module 300 , so that the middle module 300 presents a relatively low positive pressure. There is an opening (not shown) between the first module 100 and the intermediate module 300 to communicate with, specifically, an opening is opened on the partition wall between the first module 100 and the intermediate module 300, further, the position of the opening can be set close to The lower part of the treatment space of the first module 100 and the middle module 300, due to the existence of the pressure difference between the first module 100 and the middle module 300, the air in the first module 100 will carry the polluted gas in the first module 100 into the middle Module 300. The air outlet fan 430 is arranged at the air outlet 201 provided above the second module 200 , and is used to guide air out of the second module 200 to form a negative pressure inside the second module 200 . There is an opening (not shown) between the intermediate module 300 and the second module 200 to communicate, specifically, an opening is provided on the partition wall between the intermediate module 300 and the second module 200, further, the position of the opening can be set near The lower part of the processing space of the intermediate module 300 and the second module 200, due to the existence of the pressure difference between the intermediate module 300 and the second module 200, the air in the intermediate module 300 will carry the polluted gas in the intermediate module 300 into the second module 200 in. Finally, the pre-sequencing integrated processing system 1000 is exhausted by the air outlet fan 430 above the second module 200 .

An air deflector 302 is also provided on the intermediate module 300 . The air deflector 302 is set facing the air inlet 301 of the intermediate module 300 , and has a first end 303 close to the first module 100 and a second end 304 far away from the first module 100 . The second end 304 is located at the obliquely downward position of the first end 303, so that an angle is formed between the air deflector 302 and the air inlet 301 as a whole, and an opening toward the second module 200 is formed between the air deflector 302 and the air inlet 301 , so that the air introduced by the middle air intake fan 420 is guided to the position of the second module 200 to form a fixed flow direction.

Further, the air inlet fan 410 , the middle air inlet fan 420 and the air outlet fan 430 are all equipped with high-efficiency filters (not shown in the figure) for filtering the air entering/exiting the integrated processing system 1000 before sequencing.

In a specific example, the first module 100 is a centrifugation module, the second module is a sample processing module, and the third module is a library preparation module. Pollutants in the external air will enter the centrifuge module during sample injection, and the centrifuged samples will be sent to the adjacent sample processing module through the conveyor belt, where the cap will be removed and the blood will be separated. This process will generate aerosols, which will affect the subsequent adverse effects on the amplification reaction. The target sample (such as buffy coat fluid) separated from the sample processing module is sent to the library preparation module through a conveyor belt, and after a series of biochemical reactions, the target product such as DNB (DNA nanoball, "DNB" for short) is finally produced for sequencing. During the whole process, the centrifuge module will introduce foreign pollutants, the sample processing module will generate aerosol, and the text preparation module will generate part of the aerosol. After the air circulation system 400 is activated, as shown in Figure 3, the airflow enters the sample processing module from the centrifuge module, then enters the library preparation module from the sample processing module, and finally is discharged from the library preparation module, thereby realizing the process from sequencing sample processing to library preparation. The prepared air circulation reduces the pollution of pollutants to test samples and equipment. Proved by internal use: the centrifugation module, sample processing module and library preparation module are integrated together to form a pre-sequencing integrated processing system 1000, and an air circulation system 400 is installed on the pre-sequencing integrated processing system 1000. After the air circulation system 400 is activated , the average number of 0.5μm dust particles in the centrifugal module is reduced by 98.683%, and the number of 5μm dust particles is reduced by 98.218%. The air cleanliness in the centrifugal module reaches the ISO (07) standard; the number of dust particles in the air in the sample processing module is reduced by 98.255% on average at 0.5 μm, and the number of dust particles in 5 μm is reduced by 97.607%. The air in the sample processing module is clean The air cleanliness in the library preparation module has reached the ISO (07) standard; the average number of 0.5 μm dust particles in the library preparation module has been reduced by 65.51%, and the number of 5 μm dust particles has been reduced by 55.54%. The air cleanliness in the library preparation module has reached ISO (08 )standard.

It can be understood that the inlet fan 410, the middle inlet fan 420 and the outlet fan 430 are not limited to be arranged on the top of the first module 100, the middle module 300, and the second module 200. In other embodiments, the inlet fan 410, The middle air inlet fan 420 and the air outlet fan 430 can also be arranged on other parts of the first module 100 , the middle module 300 , and the second module 200 , such as the bottom or the back.

It can be understood that, in other implementation manners, the intermediate air intake fan provided on the intermediate module 300 may also be omitted.

Please refer to FIGS. 4-6 , which are the overall schematic diagrams of the pre-sequencing integrated processing system in the second embodiment. In this embodiment, the pre-sequencing integrated processing system 2000 includes a first module 500 , a second module 600 and an air circulation system 700 disposed on the first module 500 and the second module 600 . Wherein, the pre-sequencing integrated processing system 2000 involves processing the two processing procedures that the sequencing samples need to go through before sequencing, and the first module 500 and the second module 600 respectively execute one of the processing procedures in sequence. The first module 500 and the second module 600 are integrated into one body. Both the first module 500 and the second module 600 have a processing space inside. The first module 500 executes the first processing process of the sequencing sample in its processing space. The second module 600 is arranged adjacent to the first module 500, and the second module 600 executes the second processing process of the sequencing sample in its processing space, and a conveying device (not shown) is arranged between the first module 500 and the second module 600, such as a conveyor belt, The sequencing samples processed by the first module 500 are sent to the intermediate module 300 through the conveyor belt to execute the second processing process. The first module 500 is provided with a channel for sequencing samples to be placed in, such as setting an openable and closable door for sequencing samples to be placed in, and the second module 600 is provided with a channel for allowing sequencing samples to be removed, such as setting an openable door for sequencing samples to be removed. closed door etc. Of course, the channels on the first module 500 and the second module 600 can also be used for other purposes. After the sequencing sample is placed in the first module 500, the first processing procedure is executed by the first module 500, and then transferred to the second module 600 by the conveyor belt, and the second processing procedure is executed by the second module 600, and the first and second processing are completed sequentially. In-process sequencing samples are removed and continue with other processing prior to sequencing or proceed directly to sequencing.

The air circulation system 700 is placed above the pre-sequencing integrated processing system 2000 , specifically, the air circulation system 700 is placed above the first module 500 and the second module 600 . Wherein, an air inlet fan 710 is arranged above the first module 500 , and an air outlet fan 720 is arranged above the second module 600 . The air inlet fan 710 is arranged at the air inlet 501 opened above the first module 500 , and is used to introduce external air into the first module 500 through the air inlet 501 to form a positive pressure inside the first module 500 . The air outlet fan 720 is arranged at the air outlet 601 opened above the second module 600 , and is used to guide air out of the second module 600 to form a negative pressure inside the second module 600 . There is an opening (not shown) between the first module 500 and the second module 600 to communicate, specifically, an opening is provided on the partition wall between the first module 500 and the second module 600, further, the position of the opening can be set At the lower part of the processing space close to the first module 500 and the second module 600, due to the existence of the pressure difference between the first module 500 and the second module 600, the air in the first module 500 will carry the Contaminated gases enter the second module 600 . Finally, the pre-sequencing integrated processing system 2000 is exhausted by the air outlet fan 720 above the second module 600 .

Further, both the air inlet fan 710 and the outlet fan 720 are equipped with high-efficiency filters (not shown in the figure) for filtering the air entering/exiting the integrated processing system 2000 before sequencing.

It can be understood that the position of the air inlet fan 710 and the air outlet fan 720 is not limited to the top of the first module 500 and the second module 600, but can also be arranged on other parts of the first module 500 and the second module 600, such as the bottom or the back, etc. parts.

In a specific example, the first module 500 is a centrifuge module, and the second module 600 is a sample processing module. The sample centrifuged by the centrifugal module is sent to the adjacent sample processing module by the conveyor belt for corresponding processing. In another specific example, the first module 500 is a sample processing module, and the second module 600 is a library preparation module. The target sample (such as buffy coat fluid) separated by the sample processing module is sent to the library preparation module through a conveyor belt, and undergoes a series of biochemical reactions to produce target products such as DNB.

It can be understood that although the above only exemplifies the integration of two or three modules, the air circulation of the integrated system can be reduced by setting the air circulation system at the same time. However, in other implementations, more modules can be integrated, for example, four modules can be integrated. At this time, the setting of the air circulation system can refer to the pre-sequencing integrated processing system 1000, where the fans on the first and last modules The settings can refer to the first module 100 and the second module 200 respectively, and the settings of the fans and wind deflectors on the middle module can refer to the middle module 300. The power of the intake fan is gradually reduced from the first module, so that the integrated system The airflow inside forms a fixed flow direction and finally exits the integrated system.

In summary, the utility model integrates two or more modules that process samples before sequencing to form an integrated processing system before sequencing, which improves equipment integration, effectively reduces floor space and staffing, and greatly improves data output. out. In addition, the pre-sequencing integrated processing system is also equipped with an air circulation system for air circulation and airflow guidance, so that the generated aerosol and other pollution sources are discharged from the integrated system, reducing pollution.

Those of ordinary skill in the art should recognize that the above embodiments are only used to illustrate the utility model, rather than as a limitation of the utility model, as long as within the spirit of the utility model, the above implementation methods Appropriate changes and changes all fall within the scope of protection of the utility model.

Claims (10)

1. An integrated processing system before sequencing, characterized in that it comprises a first module performing a first processing procedure of a sequencing sample, a second module performing a second processing procedure of a sequencing sample, and an air circulation system, the first module It is adjacent to the second module and has a processing space inside. The processing spaces of the first module and the second module have openings to communicate with each other. The air circulation system includes an air inlet arranged on the first module. The fan and the outlet fan arranged on the second module, the inlet fan introduces external air into the processing space of the first module, so that a positive pressure is formed in the processing space of the first module, and the outlet fan The blower exhausts the air in the processing space of the second module from the integrated processing system before sequencing, so that a negative pressure is formed in the processing space of the second module, and the processing space of the first module and the processing space of the second module The pressure difference between them makes the air flow enter the processing space of the second module from the processing space of the first module, and the integrated system before sequencing is exhausted by the air outlet fan, thereby exhausting the first module and the second module. The second module treats pollutants in the space. 2. The integrated processing system before sequencing according to claim 1, wherein the air inlet fan is arranged on the top of the first module, and/or the outlet fan is arranged on the top of the second module. top. 3. The integrated processing system before sequencing according to claim 1, wherein a filter is provided in the air inlet fan to filter the air entering the first module, and/or a filter is provided in the outlet fan A filter filters the air exiting the integrated pre-sequencing processing system. 4 . The pre-sequencing integrated processing system according to claim 1 , wherein the first module and the second module transfer the sequencing samples from the first module to the second module through a transfer device. 5. An integrated pre-sequencing processing system, characterized in that it includes a first module that executes the first processing procedure of the sequencing sample, a second module that executes the second processing procedure of the sequencing sample, and performs the processing between the first processing procedure and the second processing procedure. The intermediate module and the air circulation system of the intermediate process between the processing processes, the first module, the intermediate module and the second module are all provided with a processing space inside, the first module and the intermediate module are adjacent to each other and the processing space The second module of the intermediate module is arranged adjacently and has an opening communication between the processing spaces. The air circulation system includes an air intake fan arranged on the first module and a fan arranged on the second module. An air outlet fan, the air inlet fan introduces external air into the processing space of the first module to form a positive pressure in the processing space of the first module, and the air outlet fan introduces external air into the processing space of the second module The air discharged from the pre-sequencing integrated processing system creates a negative pressure in the processing space of the second module, and the pressure difference between the processing space of the first module and the second module makes the air flow from the processing space of the first module enter the processing space of the middle module, then enter the processing space of the second module from the middle module, and finally discharge the pre-sequencing integrated system by the air outlet fan, so as to discharge the second The pollutants in the space are treated by the first module, the middle module and the second module. 6. The integrated processing system before sequencing according to claim 5, wherein the air circulation system also includes an intermediate air intake fan arranged in the intermediate module, and the intermediate air intake fan introduces external air into the In the processing space of the intermediate module, a positive pressure is formed in the processing space of the intermediate module, and the positive pressure is lower than the positive pressure in the processing space of the first module. 7. The pre-sequencing integrated processing system according to claim 5 or 6, wherein the number of the intermediate modules is one or more. 8. The integrated processing system before sequencing according to claim 6, wherein an air deflector is arranged on the intermediate module, and the air deflector is arranged facing the air inlet of the intermediate module, and the air deflector will The external air introduced by the middle air intake fan is guided to the position where the second module is located. 9. The integrated processing system before sequencing according to claim 5, wherein the first module and the intermediate module transfer the sequencing sample from the first module to the intermediate module through a transmission device; and the The intermediate module and the second module transfer the sequencing sample from the intermediate module to the second module through the transfer device. 10. The pre-sequencing integrated processing system according to claim 5 or 6, wherein the first module is a centrifugation module, the intermediate module is a sample processing module, and the second module is a library preparation module.