CN102337210B - Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment - Google Patents
Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment Download PDFInfo
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- CN102337210B CN102337210B CN 201110304498 CN201110304498A CN102337210B CN 102337210 B CN102337210 B CN 102337210B CN 201110304498 CN201110304498 CN 201110304498 CN 201110304498 A CN201110304498 A CN 201110304498A CN 102337210 B CN102337210 B CN 102337210B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention provides positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment, which comprises a positive pressure dustless aseptic operation cabinet body provided with a high-efficiency filtering air supply system, and an anti-pollution aerosol generating negative pressure shielding cabinet body arranged on the other side, wherein an ultraviolet germicidal lamp and a gas steaming and ozone sterilizer are arranged in the positive pressure dustless aseptic operation cabinet body; the positive pressure dustless aseptic operation cabinet body is used for cell exposure aseptic toxicant exposure experiments after being sterilized; the anti-pollution aerosol generating negative pressure shielding cabinet body with a suction function is used for the aerosol generation and quantitative transmission of toxicant exposure substances and waste gas exhaust; the two cabinet bodies are closely connected and are communicated with each other through a pipeline and a valve, and the bottom of the cabinet is provided with an openable movable clapboard; and front surfaces of the two cabinet bodies are provided with glass shielding windows capable of vertically opened or closed in a sliding way. The positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment has the advantages that: in a combined cabinet body, the negative cabinet body sucks air inwards to protect the safety of operators and complete the operation of toxic substances, the positive pressure cabinet body realizes the dustless aseptic environment required by the cell exposure experiments through high-efficiency filtering outward air supply, and new equipment for safely completing scientific experiments of cell exposure in aerosol samples is provided.
Description
Technical field:
The invention provides a kind of positive-negative pressure compatible cell and expose the aseptic shielding cabinet equipment.
Background technology:
It is in recent years in bio-science that the aerosol of cell exposes exposure experiment, medical and health, emerging experimental technique in animals and plants and the toxicologic study, it is the use-testing animal not, the method that only substitutes with cell or local organization is carried out the scientific experiment that the cell aerosol exposes, the scientific experiment that this cell aerosol exposes can be applied to cell interior mechanism, deep experimentation not only convenient succinctly but also scientific and precise, experimental data is accurately careful, can also shorten that the test period saves time and resource, international and domestic extensive and far-reaching practicality and researching value be arranged; Exposing at the aerosol of cell needs one can guarantee that cleaning that cell survives normally, grow and test, dustless, meanwhile aerosol exposure of gnotobasis are again need to some poisonous and hazardous materials are aerosolized in the exposure experiment, be easy to diffusion and operating environment of pollution, even the operator is damaged; This is one had not only needed the contradiction that links together but also can not hold mutually.Also not have in the industry at present satisfactorily to finish specially the required professional equipment of this type of test, can't make things convenient for, safety, the aerosol that carries out cell accurately, reliably exposes exposure experiment, thus the going deep into and develop of this type of scientific research that has restricted; Therefore design and develop a kind of positive-negative pressure compatible cell exposure aseptic shielding cabinet equipment and just become current urgent needs.
Summary of the invention:
For addressing the above problem, the purpose of this invention is to provide a kind of positive-negative pressure compatible cell and expose the aseptic shielding cabinet equipment, but expose disinfection in the exposure experiment and through the positive pressure operation cabinet of the cleaning of high efficiency filter forced draft, dustless, gnotobasis, survive normally, grow and test to guarantee cell at the aerosol of cell.Same the other side is that an antipollution aerosol generation negative pressure with suction function shields cabinet, the aerosol of finishing hazardous and noxious substances occurs, transmits and the contamination exposure test, and whole shielding cabinet bottom can be with safety dumping after some poisonous and hazardous material adsorption treatment of aerosol exposure generation with the waste gas absorbing treatment device of negative pressure-pumping effect.This positive-negative pressure compatible cell exposes aseptic shielding cabinet energy safety, aerosol accurate, convenient, that satisfactorily finish cell required in bio-science, medical and health, animals and plants and the toxicologic study reliably exposes exposure experiment, can be applied in widely in the scientific research in a plurality of fields such as toxicity, biology, medical treatment, environmental protection, military affairs, chemical industry.
For achieving the above object, technical scheme of the present invention is that a kind of positive-negative pressure compatible cell exposes the aseptic shielding cabinet equipment, described equipment comprises that with the positive pressure operation cabinet of high efficiency filter forced draft system dust-free sterile and the other side be the associating two-phase cabinet that shields the cabinet composition with the anti-pollution aerosol generation negative pressure of suction function, wherein with the dust-free sterile positive pressure operation cabinet inside of high efficiency filter forced draft system ultraviolet germicidal is housed, gas fumigation and ozonateur, the rear placement cell exposure contamination frame that can be used to carry out disinfection carries out cell and exposes aseptic viral infection test, the blast inlet that is installed in the top in the whole experimentation filters by high efficiency filter forced draft system ceaselessly outwards carries the dust-free sterile fresh air from top to bottom in the positive pressure operation cabinet, form positive pressure environment and can prevent effectively that the ambient atmos intrusion from causing bacterial contamination in this operation cabinet; And the other side is the anti-pollution aerosol generation negative pressure shielding cabinet with suction function, inside is placed with contamination material aerosol dispenser and transmission valve and pipeline be used for the contaminating aerosol of material and occurs, quantitatively transmission and waste gas are discharged at the inner rear of negative pressure shielding cabinet has the negative pressure suction opening to be connected to whole shielding cabinet bottom with the waste gas absorbing treatment device of negative pressure-pumping effect by the negative pressure pipe connecting, ceaselessly aspirating air in the cabinet in whole experimentation makes negative pressure shielding cabinet inside keep at any time negative pressure state with respect to the external world, the toxic and harmfuls such as contamination aerosol in the negative pressure shielding cabinet can not leak to pollute operator and extraneous space but directly is inhaled into and carry out safety row after the harmless treatment in the waste gas absorbing treatment device; Positive pressure operation cabinet and two cabinets of negative pressure shielding cabinet closely link to each other, and are middle by pipeline and valve connection, but the active clapboard of folding is equipped with in the central dividing plate bottom, and two cabinet front upper have the tensimeter that shows the inside and outside pressure reduction of cabinet separately; Have separately in the middle of two cabinet fronts and can slide up and down the glass port of opening or closing and be used for effectively air-isolation and handled easily personnel and carry out test operation.
Described positive pressure operation cabinet and the other side with high efficiency filter forced draft system dust-free sterile is the associating two-phase cabinet that shields the cabinet composition with the anti-pollution aerosol generation negative pressure of suction function, be that framework is made with metal sheet and section bar in its outside of experimental implementation cabinet made from metallic substance, the inner liner that adopts stainless steel plate to make can effectively prevent from polluting and be conducive to sterilize and cleaning; Positive pressure operation cabinet inside is equipped with cell and is exposed the contamination frame and culture dish and the incubation pond that holds the cell lamina membranacea arranged and expose the chamber and be used for carrying out cell and expose exposure experiment, in the whole experimentation by the blast inlet at positive pressure operation cabinet top by gasket be equipped with forced draft system with high efficiency filter with the outside air filtration sterilization after continuously input form positive pressure environment, the ultraviolet germicidal, gas fumigation and the ozonateur that are equipped with in positive pressure operation cabinet inside with automatic timing switch can be to the sterilizations that carries out disinfection of cabinet internal timing; Anti-pollution aerosol generation negative pressure shielding cabinet with suction function, inside is placed with contamination material aerosol dispenser and comprises the quantitative input control device of contamination material, contamination material aerosol vaporizer and aerosol and collect transmission interface, passes central dividing plate by transmission valve and pipeline and is connected to positive pressure operation cabinet inside and the exposure chamber that cell exposes on the contamination frame is housed cell is carried out exposure experiment.
The invention has the beneficial effects as follows: provide a kind of positive-negative pressure compatible cell to expose the aseptic shielding cabinet equipment, exposed in the exposure experiment at the aerosol of cell and not only can guarantee that cell under cleaning, dustless, the gnotobasis is survived normally, grow simultaneously but also the cell contamination that can finish safely hazardous and noxious substances exposes and tests; Solved this compatible difficult problem that links together but also can not pollute mutually of not only having needed; Can be safely, accurately, aerosol convenient, that satisfactorily finish cell required in the toxicologic study reliably exposes exposure experiment, finish the scientific experiment that cell is exposed under the aerosol sample for safety in the wide spectrums such as toxicity, biology, medical treatment, environmental protection, military affairs, chemical industry new installation be provided.
Description of drawings:
Accompanying drawing 1 is looked schematic diagram for the master of structure of the present invention
Accompanying drawing 2 is the schematic top plan view of structure of the present invention
Accompanying drawing 3 is the schematic side view of structure of the present invention
Wherein among the figure:
1, negative pressure shielding cabinet
2, positive pressure operation cabinet
3, high efficiency filter forced draft system
4, ultraviolet germicidal
5, gas fumigation and ozonateur
6, cell exposes the contamination frame
7, expose the chamber
8, culture dish
9, warm bathing pool
10, waste gas absorbing treatment device
11, negative pressure suction opening
12, aerosol dispenser
13, quantitative input control device
14, vaporizer
15, collect transmission interface
16, glass port
17, tensimeter
18, central dividing plate
19, blast inlet
20, active clapboard
21, negative pressure pipe connecting
Embodiment:
By reference to the accompanying drawings 1, accompanying drawing 2 and accompanying drawing 3 and embodiment are illustrated the present invention.
Described equipment comprises that with positive pressure operation cabinet 2 bodies of high efficiency filter forced draft system 3 dust-free sterile and the other side be the associating two-phase cabinet that anti-pollution aerosol generation negative pressure shielding cabinet 1 body with suction function forms, wherein with the dust-free sterile positive pressure operation cabinet 2 body inside of high efficiency filter forced draft system 3 ultraviolet germicidal 4 is housed, gas fumigation and ozonateur 5, the rear placement cell exposure contamination frame 6 that can be used to carry out disinfection carries out cell and exposes aseptic viral infection test, the blast inlet 19 that is installed in the top in the whole experimentation is ceaselessly outwards carried the dust-free sterile fresh air from top to bottom by the powered air-purifying effect with high efficiency filter forced draft system 3 in positive pressure operation cabinet 2 bodies, form positive pressure environment and can prevent effectively that the ambient atmos intrusion from causing bacterial contamination in this operation cabinet; And the other side is anti-pollution aerosol generation negative pressure shielding cabinet 1 body with suction function, inside is placed with contamination material aerosol dispenser 12 and transmission valve and pipeline be used for the contaminating aerosol of material and occurs, quantitatively transmission and waste gas discharge work, there is negative pressure suction opening 11 to be connected to whole shielding cabinet bottom with the waste gas absorbing treatment device 10 of negative pressure-pumping effect by negative pressure pipe connecting 21 at the inner rear of negative pressure shielding cabinet 1 body, ceaselessly aspirate air in the cabinet and make negative pressure shielding cabinet 1 body inside keep at any time with respect to the negative pressure state in the external world and the air flowing of ecto-entad is arranged in whole experimentation, the toxic and harmfuls such as contamination aerosol in negative pressure shielding cabinet 1 body just can not leak pollution operator and extraneous space but directly be inhaled into and carry out safety dumping after the harmless treatment in the waste gas absorbing treatment device 10; Two cabinets of positive pressure operation cabinet 2 bodies and negative pressure shielding cabinet 1 body closely link to each other, middle by pipeline and valve connection, but 20, two cabinet front upper of active clapboard of folding are equipped with in central dividing plate 18 bottoms the tensimeter 17 that shows the inside and outside pressure reduction of cabinet is arranged separately; Have separately in the middle of two cabinet fronts and can slide up and down the glass port 16 of opening or closing and be used for effectively air-isolation and handled easily personnel and carry out test operation.
Described positive pressure operation cabinet 2 bodies and the other side with high efficiency filter forced draft system 3 dust-free sterile is the associating two-phase cabinet that anti-pollution aerosol generation negative pressure shielding cabinet 1 body with suction function forms, be that framework is made with metal sheet and section bar in its outside of experimental implementation cabinet made from metallic substance, the inner liner that adopts stainless steel plate to make can effectively prevent from polluting and be conducive to sterilize and cleaning; Positive pressure operation cabinet 1 body inside is equipped with cell and is exposed contamination frame 6 and culture dish 8 and the incubation pond 9 that holds the cell lamina membranacea arranged and expose chamber 7 and be used for carrying out cell and expose exposure experiment, positive pressure operation cabinet 2 body tops have blast inlet 19 by gasket be equipped with forced draft system 3 with high efficiency filter with the outside air filtration sterilization after continuously input, the ultraviolet germicidal 4, gas fumigation and the ozonateur 5 that are equipped with in positive pressure operation cabinet 2 body inside with automatic timing switch can be to the sterilizations that carries out disinfection of cabinet internal timing; Anti-pollution aerosol generation negative pressure shielding cabinet 1 body with suction function, inside is placed with contamination material aerosol dispenser 12 and comprises the quantitative input control device 13 of contamination material, contamination material aerosol vaporizer 14 and aerosol and collect transmission interface 15, passes central dividing plate 18 by transmission valve and pipeline and is connected to positive pressure operation cabinet 2 body inside and 7 pairs of cells in exposure chamber that cell exposes on the contamination frame 6 are housed carry out exposure experiment.
Embodiment:
Power-on at first when using a kind of positive-negative pressure compatible cell of the present invention to expose the aseptic shielding cabinet equipment, down sliding is closed glass port 16 and is used successively ultraviolet germicidal 4, cleaning-sterilizing is carried out in 5 pairs of positive pressure operation cabinets of gas fumigation and ozonateur, 2 body inside, open whole shielding cabinet bottom and make the inner negative pressure-pumping function that produces of negative pressure shielding cabinet 1 body with the waste gas absorbing treatment device 10 of negative pressure-pumping effect, make negative pressure shielding cabinet 1 body inside keep at any time with respect to the negative pressure state in the external world and the air flowing of ecto-entad is arranged, the toxic and harmfuls such as contamination aerosol in negative pressure shielding cabinet 1 body just can not leak and pollute operator and extraneous space; Make high efficiency filter forced draft system 3 ceaselessly in positive pressure operation cabinet 2 bodies, outwards carry the dust-free sterile fresh air from top to bottom 3 unlatchings of high efficiency filter forced draft system, in this operation cabinet, form positive pressure environment and can prevent effectively that the ambient atmos intrusion from causing bacterial contamination; Upward sliding is opened positive pressure operation cabinet 2 body glass ports 16, open cell and expose contamination frame 6 and will test used cell lamina membranacea and put into culture dish 8 and incubation pond 9 and expose chamber 7 interior rear airtight good, down sliding is closed the glass port 16 of positive pressure operation cabinet 2 bodies in order to carry out cell exposure exposure experiment; Upward sliding is opened the glass port 16 of negative pressure shielding cabinet 1 body, the required material that exposes of contaminating is put in quantitative input control device 13 containers of contamination material of contamination material aerosol dispenser 12, set quantitative input speed and open contamination material aerosol dispenser 12 switches, the glass port 16 that down sliding is closed negative pressure shielding cabinet 1 exposes exposure experiment; The aerosol that produces during 14 work of contamination material aerosol vaporizer enters into aerosol and collects transmission interface 15 and be connected to transmission valve and pipeline passes central dividing plate 18 and enters into positive pressure operation cabinet 2 body inside and 7 pairs of cells in exposure chamber that cell exposes on the contamination frame 6 are housed expose exposure experiment; First upward sliding is opened negative pressure shielding cabinet 1 body glass port 16 after the experiment knot speed, close contamination material aerosol dispenser 12 switches, stop 14 work of aerosol vaporizer, upward sliding is opened the glass port 16 of positive pressure operation cabinet 2 bodies, opens cell and exposes that contamination frame 6 will test used cell lamina membranacea from culture dish 8 and incubation pond 9 and exposure chamber 7 interior taking-ups are analyzed and post-processed work; Close respectively high efficiency filter forced draft system 3 and with the waste gas absorbing treatment device 10 of negative pressure-pumping effect, two cabinet cleaning insides are clean, and down sliding is closed the glass port 16 rear powered-down switches of two cabinets respectively.
Claims (3)
1. a positive-negative pressure compatible cell exposes the aseptic shielding cabinet, it is characterized in that: described equipment comprises that the positive pressure operation cabinet (2) of band high efficiency filter forced draft system (3) and the other side are the associating two-phase cabinets with the negative pressure shielding cabinet (1) of suction function composition, it is an experimental implementation cabinet made from metallic substance, its outside metal sheet and section bar with spraying is made framework, and the inner stainless steel plate that adopts is made liner; Wherein ultraviolet germicidal (4), gas fumigation and ozonateur (5) are equipped with in positive pressure operation cabinet (2) inside, the inner cell of placing of positive pressure operation cabinet (2) exposes contamination frame (6), by the culture dish that holds the cell lamina membranacea (8) and incubation pond (9) and expose chamber (7) and form, culture dish (8) is surrounded by incubation pond (9), expose chamber (7) be installed in culture dish (8) and cell lamina membranacea directly over, expose chamber (7) and be connected on the aerosol collection transmission interface (15) in the negative pressure shielding cabinet (1) by pipeline and valve; High efficiency filter forced draft system (3) outwards carries the dust-free sterile fresh air from top to bottom in positive pressure operation cabinet (2), form positive pressure environment in this operation cabinet; And the negative pressure shielding cabinet (1) of the other side, the inner contamination material aerosol dispenser (12) of placing, wherein comprise the contamination quantitative input control device of material (13), contamination material aerosol vaporizer (14) and aerosol are collected transmission interface (15), pass central dividing plate (18) by transmission valve and pipeline and be connected to positive pressure operation cabinet (2) inside and cell is housed exposes in the exposure chamber (7) on the contamination frame (6), the waste gas absorbing treatment device (10) that the inner rear of negative pressure shielding cabinet (1) has negative pressure suction opening (11) to connect with the negative pressure-pumping effect produces negative pressure; (1) two cabinet of positive pressure operation cabinet (2) and negative pressure shielding cabinet closely links to each other, be communicated with by pipeline and valve, the junction arranges central dividing plate (18), but the active clapboard (20) of folding is equipped with in the bottom, and two cabinet front upper have the tensimeter (17) that shows the inside and outside pressure reduction of cabinet separately; Have separately in the middle of two cabinet fronts and can slide up and down the glass port (16) of opening or closing.
2. positive-negative pressure compatible cell according to claim 1 exposes the aseptic shielding cabinet, it is characterized in that: the high efficiency filter forced draft system (3) that install by gasket on positive pressure operation cabinet (2) top of band high efficiency filter forced draft system (3) dust-free sterile is a rectangular seal metal enclosed cabinet, its inner high efficiency filter adopts macromolecule filter material and built-in assembling to be integral, its inlet end has a blast inlet (19) to be connected to the opening part at positive pressure operation cabinet (2) top, and high efficiency filter forced draft system (3) outlet side is connected air-supply downwards with gasket all around with positive pressure operation cabinet (2).
3. positive-negative pressure compatible cell according to claim 1 exposes the aseptic shielding cabinet, it is characterized in that: have negative pressure suction opening (11) to be connected to by negative pressure pipe connecting (21) on the waste gas absorbing treatment device (10) of whole shielding cabinet bottom with the negative pressure-pumping effect at the inner rear of negative pressure shielding cabinet (1), this device is comprised of negative pressure vacuum fan and the solid-state adsorber of gac, the solid-state adsorber input terminus of gac is sealably coupled to by negative pressure pipe connecting (21) on the negative pressure suction opening (11) at the inner rear of negative pressure shielding cabinet (1), and the solid-state adsorber output terminal of gac is connected on the negative pressure vacuum fan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110304498 CN102337210B (en) | 2011-10-10 | 2011-10-10 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110304498 CN102337210B (en) | 2011-10-10 | 2011-10-10 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
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| Publication Number | Publication Date |
|---|---|
| CN102337210A CN102337210A (en) | 2012-02-01 |
| CN102337210B true CN102337210B (en) | 2013-01-23 |
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| CN 201110304498 Active CN102337210B (en) | 2011-10-10 | 2011-10-10 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6121190B2 (en) * | 2012-02-22 | 2017-04-26 | 住友重機械工業株式会社 | Hot cell |
| CN103436443B (en) * | 2013-09-05 | 2015-11-04 | 天津开发区合普工贸有限公司 | Integration zero leakage gas positive-negative pressure compatible microorganism isolated operation cabinet |
| CN107412818A (en) * | 2015-09-11 | 2017-12-01 | 洛阳佳嘉乐农产品开发股份有限公司 | A kind of efficient, the intensive ozone workplace of planting edible mushroom sterilizing |
| CN112245063B (en) * | 2020-11-18 | 2024-06-11 | 天津开发区合普工贸有限公司 | Swift quiet electric welding waste gas inhales toxicity experimental apparatus |
| CN114028595B (en) * | 2021-10-28 | 2023-09-19 | 武汉生物制品研究所有限责任公司 | Novel disinfection and evaluation method for coronavirus aerosol exposure system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1570075A (en) * | 2003-07-17 | 2005-01-26 | 友达光电股份有限公司 | Microbial cultivation sampling device |
| CN102010821A (en) * | 2010-09-02 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell gaseous exposure experiment control device |
| CN201971837U (en) * | 2011-03-28 | 2011-09-14 | 天津开发区合普工贸有限公司 | Cell radon exposure toxicant contamination experiment device |
-
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- 2011-10-10 CN CN 201110304498 patent/CN102337210B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1570075A (en) * | 2003-07-17 | 2005-01-26 | 友达光电股份有限公司 | Microbial cultivation sampling device |
| CN102010821A (en) * | 2010-09-02 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell gaseous exposure experiment control device |
| CN201971837U (en) * | 2011-03-28 | 2011-09-14 | 天津开发区合普工贸有限公司 | Cell radon exposure toxicant contamination experiment device |
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| CN102337210A (en) | 2012-02-01 |
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Address after: 300000 building 304, No. 39, Huanghai Third Street, Tianjin Economic and Technological Development Zone, Binhai New Area, Tianjin Patentee after: TIANJIN HOPE INDUSTRY & TRADE Co.,Ltd. Address before: 300020 No. 9, Xinjiang Road, Heping District, Tianjin Patentee before: TIANJIN HOPE INDUSTRY & TRADE Co.,Ltd. |