CN103636514A - Experimental animal feeding negative pressure isolator - Google Patents
Experimental animal feeding negative pressure isolator Download PDFInfo
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- CN103636514A CN103636514A CN201310648380.5A CN201310648380A CN103636514A CN 103636514 A CN103636514 A CN 103636514A CN 201310648380 A CN201310648380 A CN 201310648380A CN 103636514 A CN103636514 A CN 103636514A
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- isolator
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- air filter
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Abstract
The invention provides an experimental animal feeding negative pressure isolator which comprises an external box body and an inner cage. The inner cage is arranged inside the external box body, the external box body is provided with a fast conveying connector valve, a drain outlet, operation gloves and a ventilation system, the ventilation system comprises an exhaust air primary filter, a first level exhaust air high-efficiency filter, a second level exhaust air high-efficiency filter, a draught fan and a high-efficiency filter, the high-efficiency filter is connected to the fresh air port supplying air for the isolator and is connected to an air return port connected to the draught fan, and the draught fan is provided with an exhaust outlet. The experimental animal feeding negative pressure isolator improves integral leakproofness, simplifies the operation procedures of article forwarding, achieves the functions of on-line monitoring of the integrity of the filters and the on-line replacing of the filters, and avoids pollution to external environments when the filters are replaced.
Description
Technical field
The present invention relates to a kind of isolator, especially relate to a kind of experimental animal feeding negative pressure isolator.
Background technology
At present experimental animal feeding negative pressure isolator, for the experiment of high standard living animal, guarantees laboratory animal and extraneous reliable isolation, and can raise easily, the work such as research, excreta and corpse processing.
Existing experimental animal feeding negative pressure isolation structure is as shown in Figure 1: comprise delivery bin 31, operation gloves 32, outer case 33, powered air-purifying device 34, exhaust-air filter and blower fan 35, interior cage 36, sewage draining exit 37.
By delivery bin 31, carry out the transmission of living animal, spoil, animal feed, experimental apparatus, during each transmission, first a side door is opened, the article of need transmission are put into delivery bin, close door, then in delivery bin, carry out disinfecting action (the most frequently used VHP sterilizing flow process approximately needs 2 hours at present), after flow process subject to sterilization finishes, open opposite side door, complete article conveying flow;
Isolator built-in function is all undertaken by operation gloves 32;
Isolator outer case 33 is generally spliced by polylith corrosion resistant plate, seam crossing sealant sealing, and face glass place is sealed by conventional seals bar;
In isolator, ventilating system is comprised of new wind filter 34, exhaust-air filter and exhaust blower 35, keeps box house to form to external world mutually negative pressure during isolator operation;
Animal feeding, in the inner cage 36 of isolator, is equipped with the annexes such as tank, feed box;
Animal excrements are discharged by isolator bottom blow-down mouth 37 after simple sterilization, be transported to sterilizing cabinet again carry out thorough sterilizing with bucket or bag after collecting.
Domestic such Whole Equipment level is lower at present, has problems as described below: 1, isolator Integral sealing is poor; 2, loaded down with trivial details, the consuming time length of article transmission procedure; 3, exhaust-air filter running status is only monitored its two ends pressure reduction, can not effectively reflect its fine status, and finds that filter damages, changes in the process of filter environment to external world; When 4, animal excrements collection, transhipment, after sewage draining exit and collecting vessel disconnect, blowoff valve below is direct and extraneous exposure, to external world environment with collecting vessel interface; 5, give, exhaust outlet is all at isolator top, isolator inner air flow is not thorough, the pernicious gas of bottom discharge deposits yields is easily gathered in isolator inside, and blower fan and the installation of isolator one, and vibration, noise that blower fan produces can be to being had a negative impact by letting animals feed.The existing isolator product in domestic market can not the high infective pathogen body of full feeding infection animal requirement.
Summary of the invention
The invention provides a kind of novel experimental animal feeding negative pressure isolator, solved the poor problem of closure, and the operation inconvenience of existing isolator, have the problems such as noise, its technical scheme is as described below:
A kind of experimental animal feeding negative pressure isolator, comprise outer case, interior cage, described interior cage is arranged in outer case, described outer case is provided with quick passing interface valve, sewage draining exit, operation gloves and ventilating system, described ventilating system comprises successively connected air draft roughing efficiency air filter, one-level air draft high efficiency particulate air filter, secondary air draft high efficiency particulate air filter, blower fan and high efficiency particulate air filter, described high efficiency particulate air filter is connected with the fresh wind port to isolator air-supply, and be connected with the return air inlet being connected with blower fan, described blower fan is provided with exhaust outlet.
Described outer case adopts stainless steel plate integral to be welded, and it is provided with face glass, and the both sides of described face glass are provided with power-assisted air spring, and for facilitating face glass to open, the place, surrounding side of described face glass adopts gas-flow closure circle to seal.
Described air draft roughing efficiency air filter, one-level air draft high efficiency particulate air filter, secondary air draft high efficiency particulate air filter adopt bag to enter bag to go out structure, the risk of Pollutants Diffusion when reducing filter replacement.
Described high efficiency particulate air filter is arranged on the top of described isolator, and the exhaust outlet of described isolator is connected to air draft roughing efficiency air filter by exhaust duct, and the junction of described exhaust duct and isolator is provided with the section of being bent upwards.
The interior cage of described isolator is provided with automatic drinking bowl, feed box, the movable structure of the rear plate of described interior cage for being driven by cylinder.
Described sewage outfalls setting, in the bottom of isolator, is connected to the continuous bag system of isolator bottom by collection bucket.
Described quick passing interface valve is comprised of detachable valve plate and fixed valve base valve plate two parts, described detachable valve plate is for docking with quick passing interface transhipment bag or transhipment bung, described fixed valve base valve plate is arranged on described isolator, for the pass-through of isolator is airtight.
Described continuous bag system is made its compresses in length and is fixed on support to form through folding by tubular double-layer plastic bag, and the upper end of described plastic sack and isolator coupling part adopt fast-assembling chuck structure, and sack sealing is tightened with clamp in lower end.
Described one-level air draft high efficiency particulate air filter and secondary air draft high efficiency particulate air filter are provided with online dust particle detection system, for realizing the real-time monitoring of air draft high efficiency particulate air filter.
Described isolator is provided with vaporized hydrogen peroxide bactericidal unit.
This experimental animal feeding negative pressure isolator has improved the seal of isolator integral body greatly compared with existing product, simplified the operation sequence of article transhipments, realized the online replacing function of on-line monitoring and the filter of filter integrity, the pollution of environment to external world while having avoided filter replacement, optimized ventilation system design, reduced the inner harmful gas concentration of isolator, extended filter service life, the vibration having produced while having reduced isolator operation, noise is to by the adverse effect of letting animals feed, facilitated the seizure of letting animals feed, facilitated the processing of animal excrements, reduced the possibility of animal excrements to operator and external environmental.For raising high infective pathogen body infection animal, and carry out correlative study work guarantee is provided.
Accompanying drawing explanation
Fig. 1 is existing experimental animal feeding negative pressure isolator structural representation;
Fig. 2 is experimental animal feeding negative pressure isolator structural representation provided by the invention;
Fig. 3 is the structural representation of quick passing interface valve;
Fig. 4 A is the front state of gas-flow closure circle inflation in face glass gas-flow closure coil structures;
Fig. 4 B is the rear state of gas-flow closure circle inflation in face glass gas-flow closure coil structures;
Fig. 5 A is that air draft is just imitated, the bag of high efficiency particulate air filter enters bag and goes out structural representation;
Fig. 5 B while changing filter puts into old filter the schematic diagram of bag;
Fig. 5 C is the schematic diagram while changing filter, sack being cut off;
Fig. 5 D packs new filter into schematic diagram in bag while changing filter;
Fig. 5 E folds by new sack the schematic diagram of putting into inner chamber while changing filter;
Fig. 6 A is the schematic diagram of interior cage normal condition;
Fig. 6 B is the schematic diagram of interior cage trap state;
Fig. 7 A is the schematic diagram of continuous bag system excreta collection is entered continuous bag inner bottom part;
Fig. 7 B will collect excreta part sack and the top schematic diagram that bag separates continuously;
Fig. 7 C is the schematic diagram that the continuous bag of continuous bag system is cut off.
Embodiment
Experimental animal feeding negative pressure isolator provided by the invention as shown in Figure 2, comprise outer case 5, interior cage 12, described interior cage 12 is arranged in outer case 5, described outer case 5 is provided with quick passing interface valve 2, sewage draining exit 6, operation gloves 4, vaporized hydrogen peroxide disinfection system and ventilating system, described ventilating system comprises successively connected air draft roughing efficiency air filter 7, one-level air draft high efficiency particulate air filter 8, secondary air draft high efficiency particulate air filter 9, blower fan 10 and high efficiency particulate air filter 1, described high efficiency particulate air filter 1 is connected with the fresh wind port 13 to isolator air-supply, and be connected with the return air inlet 14 being connected with blower fan 10, described blower fan 10 is provided with exhaust outlet 15.Described vaporized hydrogen peroxide disinfection system comprises vaporized hydrogen peroxide sterilizing inlet device 16 and vaporized hydrogen peroxide sterilizing outlet device 17.
By quick passing interface valve 2 and quick passing interface transhipment bag 3 or quick passing interface transferring barrel 21, support the use, carry out the transmission of living animal, spoil, animal feed, experimental apparatus.Described quick passing interface is Rapid Transfer Ports, simplicity is RTP, be a kind of interface that isolator docks with mobile containers, use quick passing interface movement system to improve and transmit article efficiency, and the Integral sealing of quick passing interface valve 2 be far above common delivery bin.Contaminated article carry out sterilizing by professional sterilizer after by airtight transfer, neither affect subsequent operation, have improved again the effect of sterilizing.
Passing interface movement system structural principle as shown in Figure 3 fast: passing interface valve 2 is divided into detachable valve plate 22 and fixed valve base valve plate 23 two parts fast, detachable valve plate 22 can dock with the bung of quick passing interface transhipment bag 3 or transferring barrel 21, make quick passing interface transhipment bag 3 or transferring barrel 21 in air-tight state, passing interface valve fixed valve base valve plate 23 is installed on isolator fast, and isolator pass-through is airtight.Only have the detachable valve plate 22 of quick passing interface valve to dock with fixing valve plate and be combined into after a complete valve plate, passing interface valve just can be opened fast, make space, quick passing interface valve both sides (be isolator with transmit bucket, bag between) be connected.Close quick passing interface valve, separated passing interface valve is fast fixed and detachable valve plate 22 after, space, passing interface valve both sides is sealed respectively again fast.And during the valve plate that passing interface valve is fixed fast, 22 combinations of detachable valve plate are complete, between two valve plates, by sealing gasket, sealed, complete valve plate is opened rear middle divisional plane can not be subject to isolator internal contamination.
For transporting from the external world into the article of isolator inside, first article are put into quick passing interface transhipment bag 3 or transferring barrel 21, and with the detachable valve plate 22 of quick passing interface valve, quick passing interface transhipment bag 3 or transferring barrel 21 are sealed, then the detachable valve plate 22 of quick passing interface valve is docked to combination with fixing valve plate, after docking, can from isolator inside by quick passing interface valve, open by operation gloves 4, the article in quick passing interface transhipment bag 3 or transferring barrel 21 are transmitted in isolator.Otherwise, for needs, article are transmitted out in isolator, first with the detachable valve plate 22 of quick passing interface valve, quick passing interface transhipment bag 3 or the transferring barrel 21 of sky are sealed, with quick passing interface valve fixedly valve plate after docking, from isolator inside, open quick passing interface valve, then the article that needs passed are put into quick passing interface transhipment bag 3 or transferring barrel 21, close quick passing interface valve, the separated detachable valve plate 22 of passing interface valve fast, to be transferred in sterilizer by quick passing interface transhipment bag 3 or the transferring barrel 21 of detachable valve plate 22 sealings of passing interface valve fast, integral body is carried out sterilizing.
Isolator outer case 5 adopts stainless steel plate integral to be welded, and power-assisted air spring 11 is equipped with in face glass 24 both sides of its setting, facilitates face glass 24 to open.Face glass 24 places, surrounding side adopt gas-flow closure circle 23 to seal, and have air pressure on-line monitoring in gas-flow closure circle, hypotony automatic alarm, and when assurance isolator normally moves, panel can not be opened, and isolator Integral sealing and reliability significantly improve.
Face glass gas-flow closure coil structures is as shown in Fig. 4 A, 4B, gas-flow closure circle 25 seals from face glass 24 side surroundings, outstanding sealing effectiveness is not only provided, and gas-flow closure circle 25 states are by PLC and solenoid control, in feeding process, sealing ring remains inflated condition, face glass 24 cannot be opened, prevent maloperation.
Isolator fresh wind port 13 adopts high efficiency particulate air filter 1 to be isolated from the outside, and air draft drains into the external world by blower fan 10 after air draft roughing efficiency air filter 7, one-level air draft high efficiency particulate air filter 8, secondary air draft high efficiency particulate air filter 9 three-stage filtrations.Just effect, high efficiency particulate air filter all adopt bag to enter bag to go out structure, the risk of Pollutants Diffusion while greatly reducing filter replacement.
I and II air draft high efficiency particulate air filter all carries online dust particle detection system, realizing air draft high efficiency particulate air filter monitors in real time, once find that high efficiency particulate air filter leaks and can enter bag by bag and go out structure and change online filter, stop because air draft high efficiency particulate air filter damages the situation of environment to external world, improved isolator reliability of operation.
The design of return air channel has strengthened the self-purification capacity of system, has alleviated the efficient load of new wind, has extended the efficient life-span of new wind, and the raising of long period laboratory animal is achieved.
Isolator air outlet upper, air draft under design, can effectively prevent that bottom animal excrements from producing pernicious gas in the diffusion of isolator inside.The section of being bent upwards of described exhaust duct 18 and isolator junction, the water in the time of preventing isolator interior flushing enters in exhaust duct 18.
Air draft is just imitated, high efficiency particulate air filter bag enters bag and goes out structure as Fig. 5 A.As shown in Figure 5 B, while needing to change filter, first open fillter section access door, the sack that is sealingly fastened in access hole place is opened, unclamp filter depression bar, old filter is put into bag; As shown in Figure 5 C, after then sack being tightened with two adjacent clips, from sack being cut off between two clips, old filter is closed in bag, and fillter section inner chamber is also in air-tight state; As shown in Figure 5 D, afterwards new filter is packed in bag, sack and the sealing of fillter section access hole is fixing; As shown in Fig. 5 E, finally the sack of old filter remnants is pushed together with new filter to fillter section inside, compress filter depression bar, new sack is folded and put into inner chamber, close access door, complete filter replacement work.
The inner vacuum magnitude to external world mutually of isolator and fresh air proportion by PCL according to negative pressure value and new wind flow relatively in the isolator of setting, control the proportional-regulating air valve aperture at fresh wind port 13, exhaust outlet 15, return air inlet 14 places, realize the Automatic Control of ventilation system.
In isolator, cage 12 is equipped with automatic drinking bowl, feed box, so that letting animals feed, interior cage door is locking, prevents animal escape.The rear plate of interior cage 12 is movable structure, and as shown in Fig. 6 A, 6B, rear plate, by air cylinder driven, is convenient to drive animal to interior cage front portion, and handled easily person catches, studies animal.
Animal excrements are discharged through the sewage draining exit 6 of isolator bottom, and dirt is entered after the continuous bag system 61 in isolator bottom is collected, transported and carried out sterilizing by professional sterilizing installation by collection bucket.Adopt continuous bag system 61 to carry out collection, the transhipment of animal excrements, operator does not directly contact animal excrements, and whole collection, transport process are totally-enclosed operation.And bag length (20-50 rice) meets processing excreta demand in whole breeding cycle continuously, isolator sewage draining exit is airtight all the time, stopped situation about exposing after the each blowdown of traditional isolator sewage draining exit, will in excreta collection, transport process, to the risk of outside environmental pollution, be down to minimum.
Continuously as shown in Figure 7 A-7C, described continuous bag system 61 is through folding, its length to be greatly reduced by tubular double-layer plastic bag to bag system 61 structures, and is fixed on support and forms.Plastic sack upper end and isolator coupling part adopt fast-assembling chuck structure, and sack sealing is tightened with clamp in lower end.As shown in Figure 7 A, during use, first open isolator bottom blow-down valve, excreta collection is entered to continuous bag inner bottom part; As shown in Figure 7 B, then bag is down extracted one section out continuously, and separates collecting the continuous bag in excreta part sack and top with two adjacent clamps; As shown in Fig. 7 C, finally between two clamps, continuous bag being cut off, sack gap cross section spray disinfectant liquid disinfectant, and the excreta bag through sealing is transported to sterilizing cabinet sterilizing.
Described isolator has improved whole seal, has simplified the operation sequence of article transhipments, has realized the online replacing function of on-line monitoring and the filter of filter integrity, the pollution of environment to external world while having avoided filter replacement.
Claims (10)
1. an experimental animal feeding negative pressure isolator, it is characterized in that: comprise outer case, interior cage, described interior cage is arranged in outer case, described outer case is provided with quick passing interface valve, sewage draining exit, operation gloves and ventilating system, described ventilating system comprises successively connected air draft roughing efficiency air filter, one-level air draft high efficiency particulate air filter, secondary air draft high efficiency particulate air filter, blower fan and high efficiency particulate air filter, described high efficiency particulate air filter is connected with the fresh wind port to isolator air-supply, and be connected with the return air inlet being connected with blower fan, described blower fan is provided with exhaust outlet.
2. experimental animal feeding negative pressure isolator according to claim 1, it is characterized in that: described outer case adopts stainless steel plate integral to be welded, it is provided with face glass, the both sides of described face glass are provided with power-assisted air spring, be used for facilitating face glass to open, the place, surrounding side of described face glass adopts gas-flow closure circle to seal.
3. experimental animal feeding negative pressure isolator according to claim 1, it is characterized in that: described air draft roughing efficiency air filter, one-level air draft high efficiency particulate air filter, secondary air draft high efficiency particulate air filter adopt bag to enter bag to go out structure the risk of Pollutants Diffusion when reducing filter replacement.
4. experimental animal feeding negative pressure isolator according to claim 1, it is characterized in that: described high efficiency particulate air filter is arranged on the top of described isolator, the exhaust outlet of described isolator is connected to air draft roughing efficiency air filter by exhaust duct, and the junction of described exhaust duct and isolator is provided with the section of being bent upwards.
5. experimental animal feeding negative pressure isolator according to claim 1, is characterized in that: the interior cage of described isolator is provided with automatic drinking bowl, feed box, the movable structure of the rear plate of described interior cage for being driven by cylinder.
6. experimental animal feeding negative pressure isolator according to claim 1, is characterized in that: described sewage outfalls setting, in the bottom of isolator, is connected to the continuous bag system of isolator bottom by collection bucket.
7. experimental animal feeding negative pressure isolator according to claim 1, it is characterized in that: described quick passing interface valve is comprised of detachable valve plate and fixed valve base valve plate two parts, described detachable valve plate is for docking with quick passing interface transhipment bag or transhipment bung, described fixed valve base valve plate is arranged on described isolator, for the pass-through of isolator is airtight.
8. experimental animal feeding negative pressure isolator according to claim 6, it is characterized in that: described continuous bag system is made its compresses in length and be fixed on support to form through folding by tubular double-layer plastic bag, the upper end of described plastic sack and isolator coupling part adopt fast-assembling chuck structure, and sack sealing is tightened with clamp in lower end.
9. experimental animal feeding negative pressure isolator according to claim 1, is characterized in that: described one-level air draft high efficiency particulate air filter and secondary air draft high efficiency particulate air filter are provided with online dust particle detection system, for realizing the real-time monitoring of air draft high efficiency particulate air filter.
10. experimental animal feeding negative pressure isolator according to claim 1, is characterized in that: described isolator is provided with vaporized hydrogen peroxide bactericidal unit.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960145A (en) * | 2014-04-15 | 2014-08-06 | 葛如军 | Negative pressure experimental animal raising device |
| CN105684920A (en) * | 2016-03-23 | 2016-06-22 | 山东新华医疗器械股份有限公司 | Breed conservation isolator for genetic engineering mice |
| CN106239568A (en) * | 2016-09-13 | 2016-12-21 | 中国人民解放军军事医学科学院卫生装备研究所 | Biological safe type fowl negative pressure isolator |
| CN107182804A (en) * | 2017-07-26 | 2017-09-22 | 浙江易健生物制品有限公司 | A kind of GMP workshops animal feeding room drainage technological design |
| CN107347687A (en) * | 2017-08-14 | 2017-11-17 | 苏州市冯氏实验动物设备有限公司 | One kind operation door flip-up style bio-safety isolating device |
| CN108211571A (en) * | 2017-12-31 | 2018-06-29 | 苏州铁树医药设备有限公司 | A kind of air filter |
| CN109804935A (en) * | 2019-01-25 | 2019-05-28 | 武汉金康空调净化有限公司 | Biological safe type big animal raising device |
| CN112273325A (en) * | 2020-10-29 | 2021-01-29 | 饶洋 | Hedgehog breeding box |
| CN113519402A (en) * | 2021-06-08 | 2021-10-22 | 中国人民解放军南部战区总医院 | Multifunctional experimental animal isolator |
| CN115119759A (en) * | 2022-05-24 | 2022-09-30 | 温州医科大学 | Independent ventilation sealing cage |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103960145B (en) * | 2014-04-15 | 2016-03-30 | 葛如军 | Negative pressure laboratory animal breeding device |
| CN103960145A (en) * | 2014-04-15 | 2014-08-06 | 葛如军 | Negative pressure experimental animal raising device |
| CN105684920A (en) * | 2016-03-23 | 2016-06-22 | 山东新华医疗器械股份有限公司 | Breed conservation isolator for genetic engineering mice |
| CN106239568B (en) * | 2016-09-13 | 2018-11-20 | 中国人民解放军军事医学科学院卫生装备研究所 | Biological safe type fowl negative pressure isolator |
| CN106239568A (en) * | 2016-09-13 | 2016-12-21 | 中国人民解放军军事医学科学院卫生装备研究所 | Biological safe type fowl negative pressure isolator |
| CN107182804A (en) * | 2017-07-26 | 2017-09-22 | 浙江易健生物制品有限公司 | A kind of GMP workshops animal feeding room drainage technological design |
| CN107347687A (en) * | 2017-08-14 | 2017-11-17 | 苏州市冯氏实验动物设备有限公司 | One kind operation door flip-up style bio-safety isolating device |
| CN108211571A (en) * | 2017-12-31 | 2018-06-29 | 苏州铁树医药设备有限公司 | A kind of air filter |
| CN109804935A (en) * | 2019-01-25 | 2019-05-28 | 武汉金康空调净化有限公司 | Biological safe type big animal raising device |
| CN112273325A (en) * | 2020-10-29 | 2021-01-29 | 饶洋 | Hedgehog breeding box |
| CN113519402A (en) * | 2021-06-08 | 2021-10-22 | 中国人民解放军南部战区总医院 | Multifunctional experimental animal isolator |
| CN115119759A (en) * | 2022-05-24 | 2022-09-30 | 温州医科大学 | Independent ventilation sealing cage |
| CN115119759B (en) * | 2022-05-24 | 2023-05-30 | 温州医科大学 | Independent ventilation sealing cage |
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